CN102604909B - 定向进化构建的催化活力提高的脂肪酶突变体 - Google Patents
定向进化构建的催化活力提高的脂肪酶突变体 Download PDFInfo
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- CN102604909B CN102604909B CN 201210083905 CN201210083905A CN102604909B CN 102604909 B CN102604909 B CN 102604909B CN 201210083905 CN201210083905 CN 201210083905 CN 201210083905 A CN201210083905 A CN 201210083905A CN 102604909 B CN102604909 B CN 102604909B
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脂肪酶突变体 | 氨基酸取代位置 | K cat(/min) | K cat提高的倍数 |
出发菌株脂肪酶 | - | 1138 | 1.0 |
突变体1-1 | Ala129Ser | 1252 | 1.1 |
突变体1-2 | Lys161Arg | 1479 | 1.3 |
突变体1-3 | Thr195Tyr | 1479 | 1.3 |
突变体1-4 | Ala230Thr | 1821 | 1.6 |
突变体1-5 | Val261Gly | 1935 | 1.7 |
突变体1-6 | Lys322Arg | 1366 | 1.2 |
突变体1-7 | Ser373Asn | 1707 | 1.5 |
突变体2-1 | Ala129Ser / Lys161Arg | 2278 | 2 |
突变体2-2 | Ala129Ser / Ala230Thr | 1365 | 1.2 |
突变体2-3 | Ala129Ser / Val261Gly | 1593 | 1.4 |
突变体2-4 | Lys161Arg/Thr195Tyr | 2845 | 2.5 |
突变体2-5 | Lys161Arg / Lys322Arg | 1707 | 1.5 |
突变体2-6 | Lys161Arg / Ser373Asn | 2048 | 1.8 |
突变体2-7 | Ala230Thr /Ser373Asn | 1707 | 1.5 |
突变体2-8 | Val261Gly/ Ser373Asn | 3726 | 3.3 |
突变体3-1 | Ala129Ser/Ala230Thr /Val261Gly | 3186 | 2.8 |
突变体3-2 | Ala129Ser/Ala230Thr/Lys322Arg | 2278 | 2 |
突变体3-3 | Lys161Arg/Thr195Tyr/Ser373Asn | 2617 | 2.3 |
突变体3-4 | Lys161Arg/Ala230Thr/Ser373Asn | 2278 | 2 |
突变体3-5 | Lys161Arg/Val261Gly/Lys322Arg | 2731 | 2.4 |
突变体3-6 | Ala230Thr/Lys322Arg/Ser373Asn | 2845 | 2.5 |
突变体4-1 | Ala129Ser/Lys161Arg/Ala230Thr/ Lys322Arg | 2073 | 1.8 |
脂肪酶突变体 | 氨基酸取代位置 | K cat(/min) | K cat提高的倍数 |
出发菌株脂肪酶 | - | 1138 | 1.0 |
突变体2-1 | Ala129Ser / Lys161Arg | 2278 | 2 |
突变体2-4 | Lys161Arg/Thr195Tyr | 2845 | 2.5 |
突变体2-7 | Ala230Thr /Ser373Asn | 1707 | 1.5 |
突变体2-8 | Val261Gly/ Ser373Asn | 3726 | 3.3 |
突变体3-2 | Ala129Ser/Ala230Thr/Lys322Arg | 2278 | 2 |
突变体3-4 | Lys161Arg/Ala230Thr/Ser373Asn | 2278 | 2 |
脂肪酶突变体 | 氨基酸取代位置 | K cat(/min) | K cat提高的倍数 |
出发菌株脂肪酶 | - | 1138 | 1.0 |
突变体1-1 | Ala129Ser | 1252 | 1.1 |
突变体1-2 | Lys161Arg | 1479 | 1.3 |
突变体1-3 | Thr195Tyr | 1479 | 1.3 |
突变体1-4 | Ala230Thr | 1821 | 1.6 |
突变体1-5 | Val261Gly | 1935 | 1.7 |
突变体1-6 | Lys322Arg | 1366 | 1.2 |
突变体1-7 | Ser373Asn | 1707 | 1.5 |
突变体2-2 | Ala129Ser / Ala230Thr | 1365 | 1.2 |
突变体2-3 | Ala129Ser / Val261Gly | 1593 | 1.4 |
突变体2-5 | Lys161Arg / Lys322Arg | 1707 | 1.5 |
突变体2-6 | Lys161Arg / Ser373Asn | 2048 | 1.8 |
突变体3-1 | Ala129Ser/Ala230Thr /Val261Gly | 3186 | 2.8 |
突变体3-3 | Lys161Arg/Thr195Tyr/Ser373Asn | 2617 | 2.3 |
突变体3-5 | Lys161Arg/Val261Gly/Lys322Arg | 2731 | 2.4 |
突变体3-6 | Ala230Thr/Lys322Arg/Ser373Asn | 2845 | 2.5 |
突变体4-1 | Ala129Ser/Lys161Arg/Ala230Thr/ Lys322Arg | 2073 | 1.8 |
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CN102604909A CN102604909A (zh) | 2012-07-25 |
CN102604909B true CN102604909B (zh) | 2013-07-17 |
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Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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EP3083924B1 (en) | 2013-12-20 | 2022-08-03 | Novozymes A/S | Compositions and processes for treatment with lipases |
CN109750013B (zh) * | 2019-03-27 | 2023-01-13 | 云南师范大学 | 一种脂肪酶突变体及其制备方法和应用 |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN101544966A (zh) * | 2009-04-28 | 2009-09-30 | 江南大学 | 一种重组酵母脂肪酶的发酵制备方法 |
CN102604911B (zh) * | 2009-11-11 | 2013-05-15 | 江南大学 | 高活力华根霉脂肪酶突变体 |
CN102604910B (zh) * | 2009-11-11 | 2013-06-12 | 江南大学 | 高催化活力脂肪酶突变体 |
CN101899427B (zh) * | 2009-11-11 | 2012-05-23 | 江南大学 | 通过定向进化构建的活力提高的脂肪酶突变体 |
CN102586203B (zh) * | 2009-11-11 | 2013-04-17 | 江南大学 | 定向进化构建的催化活力提高的脂肪酶突变体 |
CN102604908B (zh) * | 2009-11-11 | 2013-01-23 | 江南大学 | 高催化活力脂肪酶突变体 |
CN101974499B (zh) * | 2010-08-13 | 2012-07-11 | 江南大学 | 热稳定性提高的脂肪酶突变体 |
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