CN102600390A - Sorghum root extract and preparation method and application thereof - Google Patents
Sorghum root extract and preparation method and application thereof Download PDFInfo
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- CN102600390A CN102600390A CN2012101028429A CN201210102842A CN102600390A CN 102600390 A CN102600390 A CN 102600390A CN 2012101028429 A CN2012101028429 A CN 2012101028429A CN 201210102842 A CN201210102842 A CN 201210102842A CN 102600390 A CN102600390 A CN 102600390A
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Abstract
The invention discloses a sorghum root extract, which contains cyanogenic glycosides with the mass percentage of 2.2-3.5 percent. The invention also discloses a preparation method and application of the sorghum root extract. The sorghum root extract has good effectiveness and safety, the price thereof is low, the preparation method is simple, and the production cost is low. Results of pharmacodynamic studies show that the sorghum root extract has effects of relieving cough, eliminating phlegm and preventing asthma and can be applied in preparation of drugs for the treatment of the respiratory diseases, and the antitussive effects of the sorghum root extract are better than the effects of codeine phosphate.
Description
Technical field
The present invention relates to a kind of Radix Sorghum vulgare Pers. extract, the invention still further relates to a kind of Radix Sorghum vulgare Pers. preparation method of extract and purposes.
Background technology
Respiratory system disease is healthy commonly encountered diseases and a frequently-occurring disease of harm humans, and the sickness rate of respiratory system disease and case fatality rate are all high in recent years.Show that according to the relevant statistics of health ministry China has more than 8,000 ten thousand people to suffer from respiratory system disease every year, and cough, expectorant, to breathe heavily be the common sympton of respiratory system disease; Especially bronchial asthma, sickness rate is higher and the course of disease is long, and is short then several years; Long then decades; And how repeatedly outbreak, particularly old people more so, so the exploitation of respiratory system disease medicine has become the focus of drug research.
Not only effect is relatively poor for the medicine of treatment respiratory system disease such as codeine, dextromethorphan etc. at present, and side effect is bigger, can produce addiction property, and cost an arm and a leg, and can not satisfy the needs of clinical treatment.It is active that Sorghum vulgare Pers. has good relieving cough and expelling phlegm, the Compendium of Material Medica record: " Zhichuan is full for liquor clothes, diuresis." " Guizhou medical herbs " record " clearing away heat-damp and promoting diuresis, reducing swelling and alleviating pain, tranquilizing the mind." Sorghum vulgare Pers. is as one of China's five big staple foods, planting range very extensively, constant is abundant, people with its seed as food consumption, and the normal abandoned of root.At present both at home and abroad not to Radix Sorghum vulgare Pers. extract and preparation method thereof with antitussive, eliminate the phlegm, the pertinent literature report of antiasthmatic effect.
Summary of the invention
First purpose of the present invention provides a kind of Radix Sorghum vulgare Pers. extract.
Second purpose of the present invention provides a kind of Radix Sorghum vulgare Pers. preparation method of extract.
The 3rd purpose of the present invention provides the purposes of Radix Sorghum vulgare Pers. extract in preparation treatment respiratory system disease medicine, especially in preparation antitussive, eliminate the phlegm, the purposes in the suppressing panting calming medicine.
Above-mentioned purpose realizes through following technical scheme:
A kind of Radix Sorghum vulgare Pers. extract contains the cyanogenic glycoside that mass percent is 2.2%-3.5% in the said extract.
A kind of Radix Sorghum vulgare Pers. preparation method of extract is used alcohol reflux with Radix Sorghum vulgare Pers., filters, and filtrating is concentrated, dry, makes and contains the extract that mass percent is the cyanogenic glycoside of 2.2%-3.5%.
Said concentration of ethanol is 80%, and the number of times of said reflux, extract, is 2-3 time, and the time is 1-2 hour.
The purposes of Radix Sorghum vulgare Pers. extract in preparation treatment respiratory system disease medicine.
The purposes of Radix Sorghum vulgare Pers. extract in the preparation antitussive medicine.
The purposes of Radix Sorghum vulgare Pers. extract in the preparation expelling phlegm drugs.
The purposes of Radix Sorghum vulgare Pers. extract in the preparation suppressing panting calming medicine.
The invention has the beneficial effects as follows: the Radix Sorghum vulgare Pers. extract effectiveness, the safety that are provided are good, cheap, and method for preparing is simple, and production cost is low.Results of pharmacodynamic test shows: the effect that Radix Sorghum vulgare Pers. extract medicine has antitussive, eliminates the phlegm, relievings asthma, can be used for preparing the medicine of treating respiratory system disease, and be that its antitussive effect has surpassed codeine phosphate.
Description of drawings
Fig. 1 detects the experiment photo of cyanogenic glycoside in the Radix Sorghum vulgare Pers. extract for Prussia's blue laws.
Fig. 2 detects the reagent paper photo of cyanogenic glycoside in the Radix Sorghum vulgare Pers. extract for Prussia's blue laws.
Fig. 3 detects the photo of cyanogenic glycoside in the Radix Sorghum vulgare Pers. extract for the picric acid test paper method.
Fig. 4 is the UV scanning figure of hydroxy benzaldehyde standard substance.
Fig. 5 is the UV scanning figure of Radix Sorghum vulgare Pers. extract alcoholic solution.
Fig. 6 is the canonical plotting of hydroxy benzaldehyde.
The specific embodiment
Below in conjunction with accompanying drawing and specific embodiment the present invention is further specified.
The preparation and the detection of embodiment 1 Radix Sorghum vulgare Pers. extract
1, preparation: get the drying in the sun Radix Sorghum vulgare Pers., shred, the alcoholic solution reflux, extract, with 80% 2 times, each 1.5h merges extracted twice liquid, filters, concentrating under reduced pressure, drying gets the Radix Sorghum vulgare Pers. extract.
2, differentiate: select for use Prussian blue experiment and picric acid test paper method that cyanogenic glycoside is differentiated.
(1) Prussian blue experiment
This experimental applications strong acid is replaced faintly acid method, with dilute sulfuric acid with CN
-Group comes out from the Radix Sorghum vulgare Pers. or the Radix Sorghum vulgare Pers. alcohol-extracted extract displace that possibly contain the cyanogen glycosides, and reuse Prussia blue laws is identified CN
-Group (Li Qiongfang and Zhang Suijuan, 2003).
The material that contains cyanide generates HCN, HCN and Fe under the strong acid effect
2+In conjunction with generating Fe (CN)
6]
4-, Fe (CN)
6]
4-Again with Fe
3+(from FeSO
4Middle Fe
2+The product of oxidation by air) generates blue Fe
4[Fe (CN)
6]
3, promptly Prussian blue.Reaction equation is following:
Fe
3++3OH
-＝Fe(OH)
3；
H
++CN
-＝HCN；
6HCN+Fe
2+＝[Fe(CN)
6]
4-+6H
+；
Fe(OH)
3+3H
+＝Fe
3++3H
2O；
3[Fe(CN)
6]
4-+4Fe
3+＝Fe
4[Fe(CN)
6]
3；
Get the seized about 2g of Radix Sorghum vulgare Pers. extract, add 10ml hot water and make its dissolving, transfer to then in the pyriform bottle, on a filter paper, drip the 20%FeSO of new preparation
41 of 2 of solution and 10%NaOH solution are then toward pyriform bottle Dropwise 5 0ml 50%H
2SO
4, in bottle, throw one piece of stirrer, will drip immediately has 20%FeSO
4The filter paper of solution and 10%NaOH solution covers on the pyriform bottle bottleneck, and makes on the filter paper wet trace align bottleneck, is placed on slowly heating on the heat collecting type magnetic agitation water-bath to the pyriform bottle then, and the trace that wets on bottle interior steam and the filter paper is fully contacted.Take off filter paper after half an hour, in the middle of filter paper, drip 2～3 of 1mol/L hydrochloric acid solutions, the change color and the experimental phenomena of record filter paper.As depicted in figs. 1 and 2, blue decorative pattern has appearred at the filter paper edge, and this phenomenon shows in the Radix Sorghum vulgare Pers. extract and has cyanogenic glycoside.
(2) picric acid test paper method
Utilization picric acid test paper method carries out composition to the Radix Sorghum vulgare Pers. extract and identifies that its principle is: cyanide is met acid and is produced hydrocyanic acid, and hydrocyanic acid and sodium picrate effect generate red special-shaped purple sour sodium.
Get the seized about 2g of Radix Sorghum vulgare Pers. extract, add 10ml hot water and make its dissolving, transfer to then in the pyriform bottle; The sulfuric acid solution of Dropwise 5 0ml 30% in conical flask; The picric acid reagent paper with the moistening slightly of 10% sodium carbonate liquor, is hanged and be hung on the pyriform bottle bottleneck of plug, the lower end of reagent paper is suspended in the middle of the pyriform bottle; Plug fixes reagent paper beyond the Great Wall immediately, observes and the record experimental phenomena.As shown in Figure 3, clearly find out among the figure to occur redness on the filter paper bar, explain in the Radix Sorghum vulgare Pers. extract and contain cyanogenic glycoside.
3, assay
(1) selection of detection wavelength
Contain in the Radix Sorghum vulgare Pers. hydroxymandelonitrile-glucoside and dhurrin (Dhurrin), dhurrin is a kind of cyanogenic glycoside, and its structure is with very similar to hydroxymandelonitrile-glucoside, their structure such as figure below:
To hydroxymandelonitrile-glucoside dhurrin
Contrast both structures, can find out that they all contain the hydroxy benzaldehyde group, can produce hydroxy benzaldehyde, glucose and hydrocyanic acid after the hydrolysis.So select for use hydroxy benzaldehyde as standard control, measure the content of cyanogenic glycoside in the Radix Sorghum vulgare Pers. extract.
Utilize hydroxy benzaldehyde in the ultra-violet (UV) band absworption peak to be arranged, its absorption curve is as shown in Figure 4, has two absworption peaks among the figure, at 221nm and 285nm maximum absorption band is arranged respectively.
Be mixed with solution to the Radix Sorghum vulgare Pers. extract with 80% ethanol; Choose suitable concentration; On ultraviolet spectrophotometer, survey its absworption peak; Absorption curve is as shown in Figure 5: among the figure, the Radix Sorghum vulgare Pers. alcoholic solution has absworption peak at the 280nm place, and this hydroxy benzaldehyde structure of just in time having coincide has absworption peak at the 285nm place.
(2) standard curve
Precision takes by weighing hydroxy benzaldehyde 2.5mg, places the 50ml small beaker, is settled in the volumetric flask of 50ml with a small amount of anhydrous alcohol solution and with dehydrated alcohol, is mixed with the hydroxy benzaldehyde solution of 50ug/ml.
Therefrom distinguish the accurate 100ul of absorption, 200ul, 400ul, 800ul, 1200ul, 1600ul hydroxy benzaldehyde alcoholic solution to the volumetric flask of 10ml with the liquid-transfering gun of 200ul; Be settled to graduation mark with dehydrated alcohol, obtain 0.5,1,2,4,6, the hydroxy benzaldehyde solution of 8ug/ml.They are measured absorbance respectively under the 285nm wavelength, the hydroxy benzaldehyde alcoholic solution of variable concentrations is seen table 1 at the light absorption value at 285nm place.
Table 1, the absorbance that each concentration hydroxy benzaldehyde solution is corresponding
Obtain the standard curve of hydroxy benzaldehyde according to above data as scatterplot, as shown in Figure 6.
The regression equation that obtains hydroxy benzaldehyde is: A=0.1325C+0.0091, R
2=0.9991.
(3) active constituent content measuring in the Radix Sorghum vulgare Pers. extract
Accurately take by weighing 0.5g Radix Sorghum vulgare Pers. extract, good with a small amount of 80% dissolve with ethanol solution, standardize solution obtains the extractum liquid of 10mg/ml in the volumetric flask of 50ml.Get the extractum liquid of 1ml, in the volumetric flask of 10ml, obtain the solution of 1mg/ml after shaking up, same method preparation 0.1mg/ml and the Radix Sorghum vulgare Pers. alcoholic solution of 0.01mg/ml with 80% ethanol standardize solution.The Radix Sorghum vulgare Pers. extract alcoholic solution of 1mg/ml, 100ug/ml, 10ug/ml is measured light absorption value at the 285nm place, the result sees table 2.
The light absorption value of the Radix Sorghum vulgare Pers. extract of table 2, variable concentrations
It is higher relatively that absorbance drops in the 0.2-0.8 scope accuracy, thus choose 0.1mg/ml the Radix Sorghum vulgare Pers. alcoholic solution measured value as a result of.The Radix Sorghum vulgare Pers. alcoholic solution light absorption value that is 100ug/ml is A=0.419, and in the substitution regression equation, the content that obtains effective ingredient cyanogenic glycoside in the Radix Sorghum vulgare Pers. extract is: 3.0936/100=3.0936%.
Radix Sorghum vulgare Pers. extract drug prepared adopts oral or injection route of administration such as (comprising intravenous injection, intravenous drip, intramuscular injection, subcutaneous injection) is carried out the treatment or the prevention of respiratory system disease.
The pharmacodynamics test of embodiment 2 Radix Sorghum vulgare Pers. extracts
One, test method
1, antitussive activity development test method
(1) the Radix Sorghum vulgare Pers. extract is to the inhibitory action of the inductive mouse cough of strong aqua ammonia
Get 70 of kunming mices, male and female half and half on desktop, place beaker with mice with a 500ml beaker back-off successively, draw ammonia 0.2ml with the 1ml syringe and inject a cotton balls of putting in the beaker, observe the cough latent period and the number of times of coughing in 3 minutes.Choose the mice of number of times between 8-20 that cough in 3 minutes and carry out next step test.
After 24 hours rehabilitation, the qualified mice of screening is divided into 5 groups, 11 every group at random.Respectively according to blank group (giving 0.5%CMC-Na), positive controls (giving the 20mg/kg codeine phosphate) is tested basic, normal, high dose groups (give respectively 50,100,200mg/kg Radix Sorghum vulgare Pers. extract), and administering mode is for irritating stomach.After the administration 1 hour, carry out strong aqua ammonia inducing mouse cough test according to method recited above.Estimate the antitussive activity of Radix Sorghum vulgare Pers. extract through cough number of times in the incubation period and 3 minutes of mouse cough before and after the contrast administration.The cough suppression ratio calculates according to following formula:
Suppression ratio (%)=(number of times of coughing after cough number of times-administration before the administration)/(cough number of times before the administration) * 100
(2) the Radix Sorghum vulgare Pers. extract is to the inhibitory action of the inductive mouse cough of capsaicin
Get 70 of kunming mices; Male and female half and half; Place one to be buckled in the bell jar of desktop mice successively; In bell jar, spray into the capsaicin solution (capsaicin concentration is 100 μ mol/L) of atomizing with ultrasound atomizer, each nebulisation time was fixed as for 10 seconds, observed the cough latent period and the number of times of coughing in 3 minutes of mice.Choose the mice of number of times between 8-20 that cough in 3 minutes and carry out next step test.
After 24 hours rehabilitation, the qualified mice of screening is divided into 5 groups, 11 every group at random.Respectively according to blank group (giving 0.5%CMC-Na), positive controls (giving the 20mg/kg codeine phosphate) is tested basic, normal, high dose groups (give respectively 50,100,200mg/kg Radix Sorghum vulgare Pers. extract) administration, and administering mode is for irritating stomach.After the administration 1 hour, carry out capsaicin inducing mouse cough test according to method recited above.The empty antitussive activity of crossing cough number of times evaluation Radix Sorghum vulgare Pers. extract in the incubation period and 3 minutes that contrasts administration front and back mouse cough.The cough suppression ratio calculates according to following formula:
Suppression ratio (%)=(number of times of coughing after cough number of times-administration before the administration)/(cough number of times before the administration) * 100
(3) the Radix Sorghum vulgare Pers. extract is to the inductive guinea pig cough's of citric acid inhibitory action
Get body weight and be 50 of the Cavia porcelluss of 200-300g, male and female half and half place one to be buckled in the bell jar of desktop Cavia porcellus successively, in bell jar, spray into 17.5% citric acid soln of atomizing with ultrasound atomizer.Each nebulisation time was fixed as 1 minute, picked up counting from spraying, observed the cough latent period and the number of times of coughing in 5 minutes of Cavia porcellus.Choose the Cavia porcellus of number of times between 8-30 that cough in 5 minutes and carry out next step test.
After 24 hours rehabilitation, the qualified Cavia porcellus of screening is divided into 5 groups, 8 every group at random.Respectively according to blank group (giving 0.5%CMC-Na), positive controls (giving the 15mg/kg codeine phosphate) is tested basic, normal, high dose groups (give respectively 25,50,100mg/kg Radix Sorghum vulgare Pers. extract) administration, and administering mode is for irritating stomach.After the administration 1 hour, carry out the inductive guinea pig cough's test of citric acid according to method recited above.Estimate the antitussive activity of Radix Sorghum vulgare Pers. extract through cough number of times in guinea pig cough's before and after the contrast administration incubation period and 5 minutes.The cough suppression ratio calculates according to following formula:
Suppression ratio (%)=(number of times of coughing after cough number of times-administration before the administration)/(cough number of times before the administration) * 100
2, expectorant activity development test method
Adopt the phenol red row's cabala of mice, with phenol red can be partly from the characteristics of trachea excretion, under the influence of expectorant; Along with the excretory increase of bronchus; By phenol red also the increasing of respiratory mucosa eliminating, measure phenol red secretory volume with spectrophotometer, thereby learn the power of medicine resolve phlegm effect.
Get 55 of kunming mices, be divided into 5 groups at random, 11 every group.Respectively according to blank group (giving 0.5%CMC-Na), positive controls (giving the 50mg/kg guaifenesin) is tested basic, normal, high dose groups (give respectively 50,100,200mg/kg Radix Sorghum vulgare Pers. extract) administration, and administering mode is for irritating stomach.After the administration 30 minutes, lumbar injection 0.5% phenol red solution 0.5ml puts to death mice (as far as possible not damaging trachea) after 30 minutes; Face upward the position and be fixed on the operation plate, cut off neck center skin, separate trachea; Place 3ml 5%NaHCO3 solution to wash, after the concussion 30min, measure absorbance at 557nm.Phenol red amount is calculated according to the phenol red-absorbance standard curve of prior formulation.
3, antiasthmatic activity development test method
Adopt histamine to draw the method for breathing heavily, get the Cavia porcellus of 200-300g, male and female all can, measure to draw one by one and breathe heavily incubation period, select eligible.Cavia porcellus is put into 402 type ultrasound atomizer, spray into the equivalent mixed liquor 12 seconds of 2% acecoline and 0.4% histamine, keep a close eye on the reaction of Cavia porcellus with the constant voltage of 53Kpa (400mmHg); Observe promptly draw incubation period that the tic of panting property appears in Cavia porcellus breathe heavily incubation period (stopping to the time that the tic of panting property occurs) from spraying should be as seeing that Cavia porcellus falls immediately with taking out; In order to avoid dead, and record draws and breathes heavily incubation period, gets to draw to breathe heavily between 30 seconds to 90 seconds, to be qualified Cavia porcellus incubation period; Be divided into 5 groups with qualified Cavia porcellus (21) at random by body weight next day; Every group 8, respectively according to blank group (giving 0.5%CMC-Na), positive controls (giving the 50mg/kg aminophylline); Test basic, normal, high dose groups (give respectively 25,50,100mg/kg Radix Sorghum vulgare Pers. extract) administration, administering mode is for irritating stomach.After the administration 1 hour, carry out histamine once more according to above-described method and draw and breathe heavily test.Breathe heavily preclinical variation through drawing before and after the contrast administration, estimate the antiasthmatic activity of Radix Sorghum vulgare Pers..
Two, result of the test
1, antitussive activity result of study
(1) the Radix Sorghum vulgare Pers. extract is to the inhibitory action of the inductive mouse cough of strong aqua ammonia.
The Radix Sorghum vulgare Pers. extract is as shown in table 3 to the inhibitory action of the inductive mouse cough of strong aqua ammonia; The same with the positive drug codeine phosphate, the Radix Sorghum vulgare Pers. extract can obviously prolong mice and draw and cough incubation period, reduces mouse cough number of times in the unit interval; And demonstrate good dose-effect relationship; 50,100, the Radix Sorghum vulgare Pers. extract of 200mg/kg has reached 22.8%, 36.9%, 50.8% respectively for the suppression ratio of cough number of times.The antitussive effect of 200mg/kg Radix Sorghum vulgare Pers. extract even surpassed the effect of positive drug codeine phosphate when 20mg/kg.
Table 3, Radix Sorghum vulgare Pers. extract are to the inhibitory action of the inductive mouse cough of strong aqua ammonia
* P＜0.05 (with the 0.5%CMC-Na ratio), * * P＜0.01 (with the 0.5%CMC-Na ratio).
#P＜0.05 (with before the administration than), ##P＜0.01 (with administration before than).
(2) the Radix Sorghum vulgare Pers. extract is to the inhibitory action of the inductive mouse cough of capsaicin.
The Radix Sorghum vulgare Pers. extract is as shown in table 4 to the inhibitory action of the inductive mouse cough of capsaicin; The same with the positive drug codeine phosphate, the Radix Sorghum vulgare Pers. extract can obviously prolong mice and draw and cough incubation period, reduces mouse cough number of times in the unit interval; And demonstrate good dose-effect relationship; 50,100, the Radix Sorghum vulgare Pers. extract of 200mg/kg has reached 24.8%, 39.6%, 52.6% respectively for the suppression ratio of cough number of times.The antitussive effect of 200mg/kg Radix Sorghum vulgare Pers. extract even surpassed the effect of positive drug codeine phosphate when 20mg/kg.
Table 4 Radix Sorghum vulgare Pers. extract is to the inhibitory action of the inductive mouse cough of capsaicin
* P＜0.05 (with the 0.5%CMC-Na ratio), * * P＜0.01 (with the 0.5%CMC-Na ratio).
#P＜0.05 (with before the administration than), ##P＜0.01 (with administration before than).
(3) the Radix Sorghum vulgare Pers. extract is to the inductive guinea pig cough's of citric acid inhibitory action.
The Radix Sorghum vulgare Pers. extract is as shown in table 5 to the inductive guinea pig cough's of citric acid inhibitory action; The same with the positive drug codeine phosphate, the Radix Sorghum vulgare Pers. extract can obviously prolong mice and draw and cough incubation period, reduces mouse cough number of times in the unit interval; And demonstrate good dose-effect relationship; 25,50, the Radix Sorghum vulgare Pers. extract of 100mg/kg has reached 24.8%, 39.6%, 52.6% respectively for the suppression ratio of cough number of times.The antitussive effect of 100mg/kg Radix Sorghum vulgare Pers. extract or even surpassed the effect of positive drug codeine phosphate when 15mg/kg.
Table 5, Radix Sorghum vulgare Pers. extract are to the inductive guinea pig cough's of citric acid inhibitory action
* P＜0.05 (with the 0.5%CMC-Na ratio), * * P＜0.01 (with the 0.5%CMC-Na ratio).
#P＜0.05 (with ratio before the administration), ##P＜0.01 (with ratio before the administration).
2, the influence of the expectorant activity of Radix Sorghum vulgare Pers. extract
Adopt the secret experiment of the phenol red row of mice to estimate the expectorant activity of Radix Sorghum vulgare Pers. extract; The Radix Sorghum vulgare Pers. extract is as shown in table 6 to the influence of the phenol red excretion amount of mice; The same with the positive drug guaifenesin, the Radix Sorghum vulgare Pers. extract can obviously increase content phenol red in the mice tracheal mucus, compares with blank; Have significant significant difference, and demonstrate good dose-effect relationship.The experiment structure shows that the Radix Sorghum vulgare Pers. extract has good expectorant activity.
Table 6, Radix Sorghum vulgare Pers. extract are to the influence of the secret amount of the phenol red row of mice trachea
* P＜0.05 (with the 0.5%CMC-Na ratio), * * P＜0.01 (with the 0.5%CMC-Na ratio)
3, the antiasthmatic activity of Radix Sorghum vulgare Pers. extract
Adopt histamine to draw the expectorant activity that the method for breathing heavily is estimated the Radix Sorghum vulgare Pers. extract, Radix Sorghum vulgare Pers. extract antiasthmatic activity as shown in table 7.Can find out that from table active than good relieving cough and expelling phlegm, the antiasthmatic activity of Radix Sorghum vulgare Pers. extract is relatively more weak.But still have certain antiasthmatic activity, when high dose, demonstrate significant difference with the blank group.
Table 7 Radix Sorghum vulgare Pers. extract draws the inhibitory action of breathing heavily to histamine
* P＜0.05 (with the 0.5%CMC-Na ratio), * * P＜0.01 (with the 0.5%CMC-Na ratio).
Claims (7)
1. a Radix Sorghum vulgare Pers. extract is characterized in that: contain the cyanogenic glycoside that mass percent is 2.2%-3.5% in the said extract.
2. Radix Sorghum vulgare Pers. preparation method of extract is characterized in that: Radix Sorghum vulgare Pers. is used alcohol reflux, filter, filtrating concentrates, dry, makes and contains the Radix Sorghum vulgare Pers. extract that mass percent is the cyanogenic glycoside of 2.2%-3.5%.
3. Radix Sorghum vulgare Pers. preparation method of extract as claimed in claim 2 is characterized in that: said concentration of ethanol is 80%, and the number of times of said reflux, extract, is 2-3 time, and the time is 1-2 hour.
4. the Radix Sorghum vulgare Pers. extract is treated the purposes in the respiratory system disease medicine in preparation.
5. the Radix Sorghum vulgare Pers. extract is in the purposes of preparation in the antitussive medicine.
6. the Radix Sorghum vulgare Pers. extract is in the purposes of preparation in the expelling phlegm drugs.
7. the Radix Sorghum vulgare Pers. extract is in the purposes of preparation in the suppressing panting calming medicine.
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| CN106729095A (en) * | 2016-12-30 | 2017-05-31 | 厦门医学院 | A kind of antifatigue medicament of Radix Sorghum vulgare Pers and preparation method thereof |
| CN107213364A (en) * | 2017-05-16 | 2017-09-29 | 贵州省中国科学院天然产物化学重点实验室 | A kind of sorghum root extract, preparation method and the usage |
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| CN106729095A (en) * | 2016-12-30 | 2017-05-31 | 厦门医学院 | A kind of antifatigue medicament of Radix Sorghum vulgare Pers and preparation method thereof |
| CN107213364A (en) * | 2017-05-16 | 2017-09-29 | 贵州省中国科学院天然产物化学重点实验室 | A kind of sorghum root extract, preparation method and the usage |
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