CN1562996A - Extract products of exopleura of sorghum, extracting method and application - Google Patents
Extract products of exopleura of sorghum, extracting method and application Download PDFInfo
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- CN1562996A CN1562996A CNA200410026844XA CN200410026844A CN1562996A CN 1562996 A CN1562996 A CN 1562996A CN A200410026844X A CNA200410026844X A CN A200410026844XA CN 200410026844 A CN200410026844 A CN 200410026844A CN 1562996 A CN1562996 A CN 1562996A
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- chinese sorghum
- exosper
- extract
- extracting method
- concentrated solution
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Abstract
This invention process consists of procedures of: raw material kaoliang external seed husk is crushed, sieve extracted, vacuum distillation concetrated, filtered, impurity removed; then, extracted, concentrated, added with crystallization agent to separate precipitate. Then it is proceeded with another purification step, to obtain extract of external seed husk of kaoliang with high anti-oxidation function. advantages are: low cost of raw material, simple prodn. process, low pollution, comerciazation prodn., its waste can be used as fodder, the product is used as health-care food benifit to liver.
Description
Technical field
The present invention relates to a kind of Chinese sorghum exosper extract, the invention still further relates to a kind of extracting method of described Chinese sorghum exosper extract and have application in the protective foods of liver protection effect in preparation.
Background technology
Pycnogenols is a class polyphenol polymer that extensively is present in the plant.After nineteen fifty-one France professor Jacques Masquelier obtained pycnogenols from the extraction of Mediterranean Sea Cortex Pini first, people constantly found the new resource that is rich in pycnogenols in nature, and its The Nomenclature Composition and Structure of Complexes is carried out Analysis and Identification.Modern biology and pharmacology prove, characteristics such as pycnogenols has nontoxic, no carcinogenic, no aberration inducing, no allergy, its resistance of oxidation far is better than vitamin-E and vitamins C, can effectively remove free radical, the protection cell exempts from the damage of free radical, thereby reduce the generation of the multiple disease that the destruction owing to free radical causes, if can reduce the incidence probability of the diseases such as retinopathy that cancer, coronary heart disease, diabetes cause; Alleviate allergic symptom, improve body immunity, slow down the process of diseases such as presenile dementia and senile cataract, reduce the sickness rate of sclerosis of artery sample and cardiovascular disorder, can also prevent the radiation injury of solar ray simultaneously skin.
At present, the extraction raw material of pycnogenols mainly contains Semen Vitis viniferae, Pericarpium Vitis viniferae, Cortex Pini etc., the main organic solvent method of its extracting method, and supercritical carbon dioxide extraction method and adsorption chromatography etc., and very few to the research of the pycnogenols of Chinese sorghum exosper.China is vast in territory, cultivated area and the output of Chinese sorghum are at the forefront in the world, make full use of the Chinese sorghum resources advantage of China, extract the pycnogenols of biologically active from its exosper, help improving the added value of farm crop Chinese sorghum, have economic benefit and social benefit widely.
Summary of the invention
The object of the present invention is to provide a kind of Chinese sorghum exosper extract that is rich in oligomeric procyanidolics.
Another object of the present invention is to provide the extracting method of this Chinese sorghum exosper extract.
The present invention also aims to provide this Chinese sorghum exosper extract to have application in the protective foods of liver protection effect in preparation.
Chinese sorghum exosper extract provided by the invention contains weight content and is no less than 95% pycnogenols, contains wherein that weight content is no less than 90%, the oligomeric procyanidolics of molecular weight between 289～1441.
The extracting method of Chinese sorghum exosper extract provided by the invention may further comprise the steps:
(1) gets Chinese sorghum and exosper thereof and pulverize, cross 10～60 mesh sieves;
(2) the adding digestion agent stirs and extracts 1～3 time, extracts 10～100 ℃ of temperature, and 1～6 hour extraction time, solid-liquid ratio 1: 1～20 filters, and concentrating under reduced pressure gets concentrated solution;
(3) the gained concentrated solution adds inorganic salt, filters or centrifugal removal solid residue, obtains clear liquid;
(4) extraction agent of getting 1～5 times of clear liquid volume extracts the gained clear liquid, repeat 1～3 time, and combining extraction liquid, concentrating under reduced pressure filters and obtains concentrated solution;
(5) add the crystallizing agent of 1～5 times of step (4) concentrated solution volume, 0 ℃～30 ℃ left standstill 3～5 hours, filtered or centrifugal collection solid, and drying obtains Chinese sorghum exosper extract.
Digestion agent of the present invention is one or more mixed solutions in water, ethanol, methyl alcohol, the acetone; Described inorganic salt are one or more mixtures in ammonium sulfate, ammonium chloride, sodium sulfate, vitriolate of tartar, sodium-chlor, calcium chloride, calcium sulfate, Repone K, SODIUMNITRATE, saltpetre, the ammonium nitrate; Described extraction agent is ethyl acetate, propyl carbinol or both mixtures; Described crystallizing agent is one or more mixtures in ether, methylene dichloride, chloroform, the sherwood oil.
Chinese sorghum exosper extract provided by the invention can be mixed with formulations such as capsule, lozenge, electuary, beverage with one or more pharmaceutically acceptable carriers (auxiliary material).
The extracting method of Chinese sorghum exosper extract provided by the invention, have the following advantages: raw material obtains easily, and is cheap; Agents useful for same is common agents, and can re-use after recovery to reduce production costs, and reduces and pollutes; Operational condition is fairly simple, is easy to realize suitability for industrialized production; Residue after the extraction also can be used as high-quality feed, has significant commercial advantage.
Show through pharmacodynamics test; Chinese sorghum exosper extract provided by the invention; can be developed into series has provide protection to alcoholic liver injury protective foods; have low price, drug effect significantly, characteristics such as instant, significant aspect assisting therapy and prevention alcoholic liver injury.
The invention will be further described below in conjunction with embodiment and pharmacodynamics test.
Embodiment
The extraction and the application of first part's Chinese sorghum exosper extract
Embodiment 1
Exsiccant Chinese sorghum exosper raw material is crushed to 40 orders for 1 kilogram; The acetone that adds 10 liter 50% stirred 3 hours in 80 ℃ of backflows of temperature, filtered or the centrifugal residue of removing concentrating under reduced pressure; Add ammonium sulfate in concentrated solution, making its final concentration is 20%, filters or the centrifugal residue of removing, and gets supernatant liquor; Add 3 liters of ethyl acetate, extract 2 times, the combined ethyl acetate layer, concentrating under reduced pressure adds anhydrous sodium sulphate again, filters and collects filtrate; Add 3 liters of sherwood oils again, 30 ℃ left standstill 4 hours, filtered or centrifugal collecting precipitation, recrystallization once again, final drying obtains about 30 gram Chinese sorghum exosper extracts, wherein contains the pycnogenols of 28.5 grams and the oligomeric procyanidolics of 27.0 grams, accounts for 95% and 90% of extract total amount respectively.
Embodiment 2
100 kilograms of Chinese sorghum exospers were pulverized 40 sieves, add 1000 liter 70% methanol solution, 50 ℃ were extracted 2 hours, filtered and removed residue, concentrating under reduced pressure; In surplus solution, add 50 kilograms of sodium-chlor, filter and collect supernatant liquor; Add 600 liters of ethyl acetate extractions, separate obtaining ethyl acetate, concentrating under reduced pressure; Add 300 liters of sherwood oils again, 4 ℃ left standstill 4 hours, filtered collecting precipitation; At recrystallization once, final drying obtains 3.2 kilograms of Chinese sorghum exosper extracts, wherein contains 3.04 kilograms pycnogenols and 2.88 kilograms oligomeric procyanidolics, accounts for 95% and 90% of extract total amount respectively.
Embodiment 3
With Chinese sorghum exosper extract of the present invention and N.F,USP MANNITOL or lactose, in 1: 4 ratio, after mixing, be pressed into particle, make Chinese sorghum exosper extract electuary through whole particle packing with liver protection effect.Adult's every day, edible 1～10 gram had good protective action to alcoholic liver injury.
Embodiment 4
With Chinese sorghum exosper extract of the present invention and glucose or lactose, in 1: 3 ratio, after mixing, packing was made the Chinese sorghum exosper extract capsule with liver protection effect.Adult's every day, edible 1～3 capsules had good protective action to alcoholic liver injury.
Embodiment 5
With Chinese sorghum exosper extract 0.5 gram of the present invention, lactose 1.0 grams, white sand Icing Sugar 0.5 gram, 10% starch syrup, 1.0 grams mix various materials by this prescription, and compressing tablet is made the Chinese sorghum exosper extract lozenge with liver protection effect.Adult's every day, edible 1～3 tablet of lozenge had good protective action to alcoholic liver injury.
Embodiment 6
With Chinese sorghum exosper extract 100 grams of the present invention, 50 kilograms of fruit juice, vitamins C 100 grams, 130 kilograms of white sugar, stablizer 100 grams, Sodium Benzoate 450 grams, 1 kilogram in water quality essence, add water to 1000 kilograms, mix sterilising packaging, make Chinese sorghum exosper extract beverage with liver protection effect.
The second section pharmacodynamics test
Chinese sorghum exosper extract provided by the invention reaches capsule, lozenge, beverage, the electuary that is prepared from by Chinese sorghum exosper extract, and alcoholic liver injury is had good protective action.Prove that through experimentation on animals effect experiment is reported as follows:
1, sample: the Chinese sorghum exosper extract capsule of liver protection effect, lozenge, beverage, electuary
2, laboratory animal: WISTER rat, body weight 150～250 grams, male and female half and half
3, test method:
(1) animal grouping: 50 rats are divided into 5 groups at random, i.e. model group, control group, large, medium and small dose of group of administration, 10 every group, male and female half and half.
(2) modeling and administration:
Control group: give boiled water, the feed of ingesting every day.
Model group: every day, ingesting was added with the feed of lard and cholesterol to 42 degree liquor.
Heavy dose of group: every day, ingesting was added with the feed of lard and cholesterol to 42 degree liquor, fed Chinese sorghum exosper extract amount: 0.20g/kg every day.
Middle dosage group: every day, ingesting was added with the feed of lard and cholesterol to 42 degree liquor, fed Chinese sorghum exosper extract amount: 0.10g/kg every day.
Small dose group: every day, ingesting was added with the feed of lard and cholesterol to 42 degree liquor, fed Chinese sorghum exosper extract amount: 0.05g/kg every day.
Each is organized aforesaid method and raised 30 days, gets hematometry liver function and lipid peroxide on the 30th day.
(3) test result
Table 1: Chinese sorghum exosper extract is to the influence of alcoholic liver injury rats'liver function and lipid peroxide (n=10, X ± S)
Group dosage (g/kg) gpt glutamic-oxal(o)acetic transaminase acid phosphatase lipid peroxide
Control group-33.42 ± 5.83 129.53 ± 19.81 13.25 ± 4.21 0.72 ± 0.55
Model group-81.58 ± 9.29
a301.19 ± 32.39
a33.47 ± 7.24
a10.12 ± 0.38
a
Heavy dose of group 0.20 69.78 ± 8.21
c239.46 ± 29.38
b16.53 ± 5.12
b1.96 ± 0.62
b
Middle dosage group 0.10 73.34 ± 7.67
c251.26 ± 36.22
c19.36 ± 6.69
b4.14 ± 0.73
b
Small dose group 0.05 77.98 ± 5.37 268.87 ± 25.71
d22.93 ± 3.72
c6.19 ± 0.63
b
Remarks: a and control group be P＜0.001 relatively; B, c, d and model group be P＜0.001 relatively, P＜0.01, P＜0.05 test-results shows:
(1) model group and control group compare, and the every biochemical indicator of rats'liver function all has considerable change, and this liver injury model success is described.
(2) heavy dose of group, middle dosage group, small dose group compare with model group, and every biochemical indicator has reduction in various degree, and the liver injury significant protective effect of Chinese sorghum exosper extract to forming is described.
(3) heavy dose of group, middle dosage group, small dose group compare with control group, and every biochemical indicator is still higher, illustrates when continuing to carry out liver injury, and the Chinese sorghum exosper extract of above dosage still can't recover the normal function of liver fully.
Claims (7)
1, a kind of Chinese sorghum exosper extract is characterized in that: contain weight content in this extract and be no less than 95% pycnogenols, contain wherein that weight content is no less than 90%, the oligomeric procyanidolics of molecular weight between 289～1441.
2, the extracting method of the described Chinese sorghum exosper of claim 1 extract may further comprise the steps:
(1) gets Chinese sorghum and exosper thereof and pulverize, cross 10～60 mesh sieves;
(2) the adding digestion agent stirs and extracts 1～3 time, extracts 10～100 ℃ of temperature, and 1～6 hour extraction time, solid-liquid ratio 1: 1～20 filters, and concentrating under reduced pressure gets concentrated solution;
(3) the gained concentrated solution adds inorganic salt, filters or centrifugal removal solid residue, obtains clear liquid;
(4) extraction agent of getting 1～5 times of clear liquid volume extracts the gained clear liquid, repeat 1～3 time, and combining extraction liquid, concentrating under reduced pressure filters and obtains concentrated solution;
(5) add the crystallizing agent of 1～5 times of step (4) concentrated solution volume, 0 ℃～30 ℃ left standstill 3～5 hours, filtered or centrifugal collection solid, and drying obtains Chinese sorghum exosper extract.
3, the extracting method of Chinese sorghum exosper extract according to claim 2 is characterized in that: the described digestion agent of step (2) is one or more mixed solutions in water, ethanol, methyl alcohol, the acetone.
4, the extracting method of Chinese sorghum exosper extract according to claim 2 is characterized in that: the described inorganic salt of step (3) are one or more mixtures in ammonium sulfate, ammonium chloride, sodium sulfate, vitriolate of tartar, sodium-chlor, calcium chloride, calcium sulfate, Repone K, SODIUMNITRATE, saltpetre, the ammonium nitrate.
5, the extracting method of Chinese sorghum exosper extract according to claim 2 is characterized in that: the described extraction agent of step (4) is ethyl acetate, propyl carbinol or both mixtures.
6, the extracting method of Chinese sorghum exosper extract according to claim 2 is characterized in that: the described crystallizing agent of step (5) is one or more mixtures in ether, methylene dichloride, chloroform, the sherwood oil.
7, the described Chinese sorghum exosper of claim 1 extract has application in the protective foods of liver protection effect in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200410026844XA CN1562996A (en) | 2004-04-15 | 2004-04-15 | Extract products of exopleura of sorghum, extracting method and application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200410026844XA CN1562996A (en) | 2004-04-15 | 2004-04-15 | Extract products of exopleura of sorghum, extracting method and application |
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| Publication Number | Publication Date |
|---|---|
| CN1562996A true CN1562996A (en) | 2005-01-12 |
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| CNA200410026844XA Pending CN1562996A (en) | 2004-04-15 | 2004-04-15 | Extract products of exopleura of sorghum, extracting method and application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389417A (en) * | 2011-07-28 | 2012-03-28 | 华中农业大学 | Preparation method and anticarious application of broomcorn proanthocyanidin mixture o |
| CN102600390A (en) * | 2012-04-10 | 2012-07-25 | 华中农业大学 | Sorghum root extract and preparation method and application thereof |
| CN104856173A (en) * | 2015-04-24 | 2015-08-26 | 南京林业大学 | A proanthocyanidin natural health-care beverage and preparation method thereof. |
-
2004
- 2004-04-15 CN CNA200410026844XA patent/CN1562996A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389417A (en) * | 2011-07-28 | 2012-03-28 | 华中农业大学 | Preparation method and anticarious application of broomcorn proanthocyanidin mixture o |
| CN102389417B (en) * | 2011-07-28 | 2013-05-01 | 华中农业大学 | Preparation method and anticarious application of broomcorn proanthocyanidin mixture o |
| CN102600390A (en) * | 2012-04-10 | 2012-07-25 | 华中农业大学 | Sorghum root extract and preparation method and application thereof |
| CN102600390B (en) * | 2012-04-10 | 2013-12-18 | 华中农业大学 | Sorghum root extract and preparation method and application thereof |
| CN104856173A (en) * | 2015-04-24 | 2015-08-26 | 南京林业大学 | A proanthocyanidin natural health-care beverage and preparation method thereof. |
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