CN1159343C - Mulberry leaf polyose and its usage - Google Patents
Mulberry leaf polyose and its usage Download PDFInfo
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- CN1159343C CN1159343C CNB011299762A CN01129976A CN1159343C CN 1159343 C CN1159343 C CN 1159343C CN B011299762 A CNB011299762 A CN B011299762A CN 01129976 A CN01129976 A CN 01129976A CN 1159343 C CN1159343 C CN 1159343C
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- mulberry leaf
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- leaf polyose
- mulberry
- amylose
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Abstract
The present invention discloses a mulberry leaf amylose product extracted from leaves of mulberries which are moraceous plants and the usage thereof. The mulberry leaf amylose product are effective section amylose with functional effects, wherein the amylose is obtained by extracting and separating the leaves of the mulberries which are the moraceous plants after the leaves are dried in the sun, the molecular weight is between 15000 to 50000 daltons, and the solid state of the mulberry leaf amylose product presents light brown or light yellow. The mulberry leaf amylose product of the present invention can be developed into series health products or foods with the blood glucose decreasing function and has the characteristics of low price and obvious drug effects; furthermore, the mulberry leaf amylose product can be eaten conveniently, etc. and has the important meaning for being supplied to diabetics and for health care and disease prevention of persons in middle and old age.
Description
Affiliated technical field
The present invention relates to a kind of mulberry leaf polyose, more particularly, the present invention relates to a kind of mulberry leaf polyose that utilizes the leaf extraction of moraceae plants mulberry tree, the invention still further relates to the purposes of this mulberry leaf polyose.
Background technology
Mulberry leaf are main raw materials of producing silk cocoon, all can plant collection throughout the year in China south, in each work-in-process of silkworm and mulberry, also be the product the easiest, that resource is the abundantest, price is the most cheap of originating, document record: the mulberry leaf tool clears liver and improve vision, sharp the five internal organs, reducing blood-fat, hypotensive, hypoglycemic, suppress effect such as cancer.But for a long time, mulberry leaf only are mainly used in sericulture, and as the Chinese medicine, many valuable ingredients also do not develop in the mulberry leaf except that on a small quantity, and resource is not fully utilized.
Summary of the invention
The object of the invention is to provide a kind of mulberry leaf polyose that utilizes the leaf extraction of moraceae plants mulberry tree.
Another object of the present invention is to provide the purposes of this mulberry leaf polyose.
Mulberry leaf polyose provided by the invention is a raw material with the leaf of moraceae plants mulberry tree, prepares by the step of following order:
(1) the flush away impurity that is soaked in water dries the back and pulverizes weighing;
(2) soak with organic solvent, remove liposoluble partly, cross the leaching slag then and remove liquid;
(3) organic solvent is removed in the slag decompression, the mulberry granulated slag bubble of gained repeats three to five times, filters, gets liquid;
(4) filtrate of step (3) gained is added organic solvent, leave standstill after the stirring, layering, remove the upper strata and contain the chlorophyll part;
(5) filtrate of step (4) gained is sloughed non-sugared organism and inorganic salt;
(6) filtrate with step (5) gained exchanges with acid ion, exchanges Deproteinization with alkali ion again;
(7) step (6) gained ion exchange liquid is added ammoniacal liquor and be neutralized to neutrality, add ethanol and form the red-brown precipitation thing;
(8) use absolute ethanol washing red-brown precipitation thing 2～3 times again, the back is the polysaccharide between 15000～50000 dalton with anionite-exchange resin and Zeo-karb intercepting molecular weight;
(9) step (8) gained liquid is concentrated back oven dry, pulverizing, promptly get the elementary polysaccharide product of these mulberry leaf.
The present invention can further purify the gains of above-mentioned steps (8), with high pressure liquid chromatography separate, purifying gets the polysaccharide of molecular weight between 15000～25000 dalton, after continue again that (9) described method is refining set by step, obtain this mulberry leaf polyose.
Used organic solvent is ethanol, methyl alcohol, Virahol, acetone, butanols, dimethyl formamide or methyl-sulphoxide in the step of the present invention (2): used organic solvent is that ethyl acetate, butylacetate, benzene, toluene or boiling range are 60～90 ℃ sherwood oil in the step (4); The method of sloughing non-sugared organism and inorganic salt in the step (5) is ion exchange resin, macroporous resin or ionodialysis method; Used Zeo-karb is 724 type Zeo-karbs or 732 type Zeo-karbs in the step (8), and anionite-exchange resin is 701 type anionite-exchange resin (used resin sees " extraction of Chinese herbal medicine effective ingredients with separate " (Shanghai People's Press) for details).
Mulberry leaf polyose molecular weight of the present invention between 15000-25000 dalton, the solid-state light brown or light yellow that is.Can be developed into the health care crystalline substance or the food of serial tool hypoglycemic activity, have low price, drug effect significantly, characteristics such as instant, offer diabetic subject and middle-aged and old health's diseases prevention to use, significant.
Embodiment
First part's polysaccharides of Folium Mori prepares example
Embodiment 1
Get 2 kilograms on the leaf of moraceae plants mulberry, be soaked in water and filtered in 1 hour, after getting the air-dry pulverizing of leaf (slag), soaked 3 hours for 60-80 ℃ with 2 times of volume water, repeat 5 times and cross leaching liquid, 80 ℃ of following evaporated under reduced pressure, dry adds acetone and stirs immersion one hour, frame leaches filtrate, adding benzene stirred 1 hour, left standstill 1 hour, and removed the upper strata after the layering and contain chlorophyll part layer liquid, slough non-sugared organism with the ionodialysis method again, protein, inorganic salt, then with acid and deacidite exchange, the gained ion exchange liquid adds ammoniacal liquor and is neutralized to neutrality, adds ethanol sedimentation and goes out polysaccharide, taking precipitate, with absolute ethanol washing throw out three times, carry with 701 anionite-exchange resin and 724 Zeo-karbs again and get the elementary polysaccharide that molecular weight is the 15000-50000 Dalton molecular weight, use macroporous resin again, high pressure liquid chromatography is separated, purify the daltonian polysaccharide of molecular weight 15000-25000.80 ℃ of oven dry are down pulverized the oven dry thing and are promptly got the little light brown polysaccharides of Folium Mori of the present invention.
Embodiment 2
2 kilograms on mulberry leaf air-dry after the impurity elimination are soaked in water intaking, add 3 times in water, 60-80 ℃ of immersion, repeat 4 times, filter, filtrate decompression is concentrated into syrupy shape, added acetone and ethyl acetate static one hour, layering goes to the upper strata to contain chlorophyll and non-polysaccharide organic matter, remove non-sugar and protein and inorganic salt with the ionodialysis method then, pass through negatively charged ion then, Zeo-karb, the gained ion exchange liquid adds the ammoniacal liquor neutralization, again by cloudy type 701 ion exchange resin, it is elementary polysaccharide between the 15000-50000 dalton that sun type 724 ion exchange resin get molecular weight, is solid-state, light brown or brown polysaccharide, at last with high pressure liquid chromatography purify the polysaccharide between the 15000-25000 dalton.80 ℃ of oven dry are down pulverized the oven dry thing and are promptly got polysaccharides of Folium Mori of the present invention.
Embodiment 3
Claim 2 kilograms with the mulberry leaf of the air-dry pulverizing of washing, repeat 3 times with 5 times of water loggings bubble, the each filtration, filtrate is concentrated concentrating under reduced pressure, adds that ethyl acetate, benzene stir respectively, extraction, static, concentrated extraction liquid.Leave standstill the organic impurity (protein, sterol thing) that contains chlorophyll and non-carbohydrate, the desalination of ion exchange resin method, the gained ion exchange liquid adds ammoniacal liquor and is neutralized to neutrality, add ethanol, there is throw out to produce, with absolute ethanol washing throw out three times, carry with 701 anionite-exchange resin and 732 Zeo-karbs again that to get molecular weight be elementary polysaccharide between the 15000-50000 dalton, separate with the high pressure liquid chromatography post then and purify to such an extent that molecular weight is a polysaccharide between the 15000-25000 dalton.Get light brown of the present invention or brown polysaccharides of Folium Mori with spray method or decompression oven drying method.
Embodiment 4
With elementary polysaccharides of Folium Mori of the present invention and nuisanceless bird dragon tea,, be prepared into mulberry leaf blood sugar reduction tea in 1: 1 ratio.Its product appearance is similar to former oolong tea, and taste has the peculiar delicate fragrance of mulberry leaf, and keeps the tealeaves original flavor.Per day for adults eats 4-6g, may command or lowering blood glucose value.
Embodiment 5
With the elementary polysaccharides of Folium Mori of the present invention and N.F,USP MANNITOL or lactose, in 1: 2 ratio, after mixing, be pressed into particle, behind whole grain, be sub-packed in water vapor proof barrier packaging material, former plastics, aluminium foil etc., make the mulberry leaf blood sugar reduction electuary.Eat 6-10g every day, may command or lowering blood glucose value.
The second section effect experiment
Polysaccharides of Folium Mori provided by the invention reaches mulberry leaf blood sugar reduction tea, the mulberry leaf blood sugar reduction electuary that is prepared from by polysaccharides of Folium Mori, has good hypoglycemic activity.Prove that through experimentation on animals effect experiment is reported as follows:
1. sample: the polysaccharides of Folium Mori of hypoglycemic activity, mulberry leaf blood sugar reduction tea, mulberry leaf blood sugar reduction electuary.
2. laboratory animal: NIH pure lines male mice is provided by Guangdong Province's medical animal feedlot.
3. test method;
(1) formation of diabetic mice: healthy male mice, body weight 20-22g went on a hunger strike 24 hours, and intravenous injection streptozocin (100mg/kg) was normally raised 20 days, and diabetes model forms.
(2) forming diabetic mice, is 1 group by 10, and under no fasting situation, every abdominal cavity injects polysaccharides of Folium Mori stoste, mulberry leaf blood sugar reduction tea, the mulberry leaf blood sugar reduction electuary of the same isoconcentration of 2ml (2%) polysaccharides of Folium Mori content respectively.Blood glucose value is surveyed in blood sampling after 6 hours.
(3) blood glucose value test; The method of glucose oxidase one peroxidase is routinely carried out.
4. test result:
(1) blood sugar decreasing effect of different molecular weight polysaccharides of Folium Mori test
Adopt different process, obtain the polysaccharides of Folium Mori of three kinds of molecular weight fragments.Through the test of streptozocin diabetic mice hypoglycemic, the results are shown in Table 1.
Table 1 different molecular weight polysaccharides of Folium Mori blood sugar decreasing effect n=10
| Positive group | 2%5000-50000 dalton polysaccharides of Folium Mori | 2%15000-25000 dalton polysaccharides of Folium Mori | 2%15000-50000 dalton polysaccharides of Folium Mori | |
| Average blood sugar value (mmol/l) | 14.15 | 12.76 | 7.96 | 9.17 |
| Average blood sugar value rate of descent (%) | / | 19.70 | 87.80 | 70.60 |
Test-results shows: 15000-25000 Dalton molecular weight polysaccharides of Folium Mori, to the diabetic mice that streptozocin forms, the blood sugar rate of descent is the highest.
(2) mulberry leaf blood sugar reduction tea blood sugar decreasing effect test
Mulberry leaf blood sugar reduction tea and former polysaccharides of Folium Mori blood sugar decreasing effect are relatively; The hypoglycemic test of the suitable working concentration of mulberry leaf blood sugar reduction tea.Result such as table 2.
Table 2 mulberry leaf blood sugar reduction tea is to the n=10 that influences of streptozocin and blood glucose in diabetic mice
| Positive group | 2% polysaccharides of Folium Mori stoste | The mulberry leaf blood sugar reduction tea of 2% polysaccharides of Folium Mori | The mulberry leaf blood sugar reduction tea of 4% polysaccharides of Folium Mori | The mulberry leaf blood sugar reduction tea of 8% polysaccharides of Folium Mori | |
| Average blood sugar value (mmol/l) | 16.76 | 9.73 | 8.83 | 7.09 | 5.57 |
| Average blood sugar rate of descent (%) | / | 72.77 | 82.1 | 100 * | 100 * |
Annotate:
*For reaching normal level
Test-results shows: the mulberry leaf blood sugar reduction tea that contains polysaccharides of Folium Mori has good blood sugar decreasing effect, and under similarity condition, hypoglycemic effect keeps original level.After diabetic mice was treated with the mulberry leaf blood sugar reduction tea that contains 2% polysaccharides of Folium Mori, the blood sugar rate of descent reached more than 80%, and result of treatment is remarkable.
(3) mulberry leaf blood sugar reduction electuary blood sugar decreasing effect test
The electuary that several excipient and polysaccharides of Folium Mori are mixed with carries out the test of hypoglycemic effect, result such as table 3.
Table 3 mulberry leaf blood sugar reduction electuary is to streptozocin diabetic mice hypoglycemic effect n=10
| Positive group | Polysaccharides of Folium Mori+N.F,USP MANNITOL | Polysaccharides of Folium Mori+lactose | Polysaccharides of Folium Mori+B-cyclodextrin | |
| Average blood sugar value (mmol/l) | 35.92 | 24.50 | 26.80 | 29.50 |
| Average blood sugar rate of descent (%) | / | 62 | 31.65 | 22.28 |
Test-results shows: the mulberry leaf blood sugar reduction electuary that several excipient form all has the obvious functions of blood sugar effect.Wherein best with the mulberry leaf blood sugar reduction electuary blood sugar decreasing effect that adopts N.F,USP MANNITOL to form.
The blood sugar rate of descent=(a-b)/(a-7.1) * 100%
A: positive group glucose level
B: test group glucose level
7.1: no fasting normal mouse glucose level (7.1mmol/L)
Claims (8)
1, a kind of mulberry leaf polyose is characterized in that this product is a raw material with the leaf of moraceae plants mulberry tree, prepares by the step of following order:
(1) the flush away impurity that is soaked in water dries the back and pulverizes weighing;
(2) soak with organic solvent, remove liposoluble partly, cross the leaching slag then and remove liquid;
(3) organic solvent is removed in the slag decompression, the mulberry granulated slag bubble of gained repeats three to five times, filters, gets liquid;
(4) filtrate of step (3) gained is added organic solvent, leave standstill after the stirring, layering, remove the upper strata and contain the chlorophyll part;
(5) filtrate of step (4) gained is sloughed non-sugared organism and inorganic salt;
(6) filtrate with step (5) gained exchanges with acid ion, exchanges Deproteinization with alkali ion again;
(7) ion exchange liquid of step (6) gained is added ammoniacal liquor and be neutralized to neutrality, add ethanol and form the red-brown precipitation thing;
(8) use absolute ethanol washing red-brown precipitation thing 2～3 times again, the back is the polysaccharide between 15000～50000 dalton with anionite-exchange resin and Zeo-karb intercepting molecular weight;
(9) step (8) gained liquid is concentrated back oven dry, pulverizing, promptly get the elementary polysaccharide product of these mulberry leaf.
2, mulberry leaf polyose as claimed in claim 1, it is characterized in that: the gains of step (8) can further be purified, get molecular weight behind the polysaccharide between 15000～25000 dalton with high pressure liquid chromatography separation, purifying, (9) described method is refining set by step in continuation again, obtains this mulberry leaf polyose.
3, mulberry leaf polyose as claimed in claim 1 is characterized in that: used organic solvent is ethanol, methyl alcohol, Virahol, acetone, butanols, dimethyl formamide or methyl-sulphoxide in the step (2).
4, mulberry leaf polyose as claimed in claim 1 is characterized in that: the method for sloughing non-sugared organism and inorganic salt in the step (5) is ion exchange resin, macroporous resin or ionodialysis method.
5, mulberry leaf polyose as claimed in claim 1 is characterized in that: used organic solvent is that ethyl acetate, butylacetate, benzene, toluene or boiling range are 60-90 ℃ sherwood oil in the step (4).
6, mulberry leaf polyose as claimed in claim 1, it is characterized in that: used Zeo-karb is 724 type Zeo-karbs or 732 type Zeo-karbs in the step (8), and used anionite-exchange resin is 701 type anionite-exchange resin.
7, the described mulberry leaf polyose of claim 1 has the Application in Food of hypoglycemic activity in processing.
8, the described mulberry leaf polyose of claim 1 has application in the health care crystalline substance of hypoglycemic activity in processing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011299762A CN1159343C (en) | 2001-11-27 | 2001-11-27 | Mulberry leaf polyose and its usage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011299762A CN1159343C (en) | 2001-11-27 | 2001-11-27 | Mulberry leaf polyose and its usage |
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| Publication Number | Publication Date |
|---|---|
| CN1351085A CN1351085A (en) | 2002-05-29 |
| CN1159343C true CN1159343C (en) | 2004-07-28 |
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| CNB011299762A Expired - Lifetime CN1159343C (en) | 2001-11-27 | 2001-11-27 | Mulberry leaf polyose and its usage |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100464764C (en) * | 2006-03-08 | 2009-03-04 | 江苏大学 | Composition of white mulberry leaf blood-sugar-reducing effective components and preparing method |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869605B (en) * | 2010-07-02 | 2012-05-02 | 安徽济人药业有限公司 | Method for extracting and separating mulberry leaf flavone and alkaloid composite |
| CN105661547A (en) * | 2016-01-22 | 2016-06-15 | 湖南营养树科技有限公司 | Probiotics and Bombyx mori compounded diabetic patient nutritional supplement |
| CN106360741A (en) * | 2016-09-13 | 2017-02-01 | 哈尔滨瑞康源生物科技有限公司 | Sugar-reducing plant composite protein powder and preparation method thereof |
| CN107137420A (en) * | 2017-07-05 | 2017-09-08 | 无限极（中国）有限公司 | A kind of mulberry leaf-chrysanthemum Chinese yam complex polysaccharide with clearing heat and nourishing yin function of blood sugar reduction and preparation method thereof |
-
2001
- 2001-11-27 CN CNB011299762A patent/CN1159343C/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100464764C (en) * | 2006-03-08 | 2009-03-04 | 江苏大学 | Composition of white mulberry leaf blood-sugar-reducing effective components and preparing method |
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| Publication number | Publication date |
|---|---|
| CN1351085A (en) | 2002-05-29 |
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Granted publication date: 20040728 |