CN102599146B - Stem cell cryopreserving box and cryopreservation method - Google Patents
Stem cell cryopreserving box and cryopreservation method Download PDFInfo
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- CN102599146B CN102599146B CN201210035051.9A CN201210035051A CN102599146B CN 102599146 B CN102599146 B CN 102599146B CN 201210035051 A CN201210035051 A CN 201210035051A CN 102599146 B CN102599146 B CN 102599146B
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Abstract
The invention discloses a stem cell cryopreserving box and a cryopreservation method. The stem cell cryopreserving box comprises a box body, a box cover and baffle plates, wherein an opening is arranged at the top part of the box body, and pressure adjusting and fixing devices are arranged outside two opposite side walls on the box body; a plurality of collection bags and a plurality of baffle plates are arranged in the box body alternately, and the baffle plates are located at the topmost part of the alternate arrangement of the collection bags and the baffle plates; and the box cover is located at the top part of the box body and is connected with the pressure adjusting and fixing devices on the side walls of the box body by fasteners. Compared with the prior art, when the stem cell cryopreserving box and the cryopreservation method are used, stem cell samples of the same patient can be placed in one box body, and the box cover can press the collection bags into a flat shape, so that stem cells can be uniformly cooled; each box body, as an independent storage unit, is convenient to take and use; and when the box body is taken and used, the operation is simple, and the consumed time is shorter, so that the refrigerator door opening time is shortened, and the temperatures of other box bodies are slightly influenced.
Description
Technical field
The application relates to Medical Devices technical field, particularly relates to the freezing storage box of a kind of stem cell and freezing and storing method.
Background technology
Stem cell is according to the difference of the required preservation time limit, is generally held in the low temperature refrigerator/liquid nitrogen container of-85 ℃ or-196 ℃.For after gathering to stem cell shorter between the operating period (< 6 months), conventionally adopt profound hypothermia refrigerator-85 ℃ frozen.Current frozen for such stem cell, there is no unified working specification, the selected storage box of constituent parts is also not quite similar, due to the easily bursting by freezing under profound hypothermia state of general rigid plastics (referring to storage box casing), so the provisional splendid attire of some box type paper materials of use mostly simply and economically gathers to the stem cell in transfusion bags.Above-mentioned store method has following shortcoming and clinical hidden danger:
(1), often will deposit a plurality of patients' stem cell in a profound hypothermia refrigerator, overlapping the discharging that different patients' stem cell can only be unordered, the signable property in position is poor, makes whole refrigerator inside seem unordered in a jumble;
(2), between different conserving cases, under low temperature environment, be usually freeze thawing tacky state, when taking-up is positioned at the conserving case of lower floor, need separately two bonding conserving cases of external force, complex operation, and this operation also makes to open the overlong time of refrigerator doors, cause ice the temperature inside the box can not be constant, affect the frozen effect of stem cell in whole refrigerator;
(3), stem cell is collected in plastics collection bag conventionally, when depositing into refrigerator, in order to keep lowering temperature uniform, often needing weight to be pressed on collection bag, to keep gathering band, be flat form, is also very strange to the selection of this weight conventionally, for example: thick books, ice cubes etc., the serious potential safety hazard of existence lack of standardization of this operation, also can kick the beam or the overweight stem cell cryopreserving quality that affects because of weight.
Summary of the invention
In view of this, the embodiment of the present application provides the freezing storage box of a kind of stem cell and freezing and storing method, to realize freezing preservation stem cell better.
To achieve these goals, the technical scheme that the embodiment of the present application provides is as follows:
The freezing storage box of stem cell, is placed on the stem cell in collection bag for freezing preservation, comprising: casing, case lid and dividing plate, wherein:
Described casing top arranges opening, and on described casing, the outside of relative two sidewalls in position is provided with pressure and regulates fixture;
A plurality of described collection bags and a plurality of described dividing plate are alternately placed in described casing, are highly greater than the degree of depth of described casing after the stack of a plurality of described collection bags and a plurality of described dividing plates, and to be positioned at top be dividing plate;
Described case lid is positioned at described casing top, and described case lid two ends are respectively arranged with buckle, described buckle one end and described case lid fix, the other end regulates fixture to be connected with the pressure on described wall box, described case lid is exerted pressure to the dividing plate of top by described buckle control, and a plurality of described collection bags are driven plain under pressure.
Preferably, described pressure regulates fixture to comprise: row's draw-in groove or row's card column, and a described draw-in groove of row or card column distribution along the vertical direction on the sidewall of described casing.
Preferably, on described wall box, be provided with the ventilation hole that a plurality of and described case intracorporeal space is connected.
Preferably, the material of described casing and dividing plate is low temperature material.
Preferably, the material of described casing and dividing plate is low temperature resistant high molecular polymer.
Preferably, on described casing lateral wall, be provided with label draw-in groove.
Preferably, between at least one sidewall of described casing and described casing, be flexibly connected.
Preferably, at least one sidewall of described casing is connected by draw-in groove connected mode or the mode that is rotationally connected with described casing.
A stem cell freezing and storing method, comprising:
A plurality of collection bags and a plurality of dividing plate are alternately placed in the casing with bottom and sidewall successively, and what be positioned at top is dividing plate;
Utilize pressure to regulate fixture and buckle to apply to dividing plate described in top the pressure that direction is pointed to described casing, a plurality of described collection bags are flattened.
Preferably, the stack of a plurality of described collection bags and a plurality of described dividing plates is highly greater than the degree of depth of described casing.
From above technical scheme, the freezing storage box of this stem cell that the embodiment of the present application provides, comprise: casing, case lid and dividing plate, wherein: on casing, the outside of relative two sidewalls in position is provided with pressure and regulates fixture, and collection bag and dividing plate are placed in casing; Case lid is positioned at casing top, be flexibly connected, and distance is between the two adjustable, and case lid can be exerted pressure to dividing plate and collection bag between case lid and casing.
Compared with prior art, the freezing storage box of this stem cell that the application provides in use, same patient's stem cell sample can be placed in a casing, and case lid can be pressed into flat by collection bag, make the stem cell can uniform decrease in temperature, and each casing be as a memory cell independently, conveniently take, and simple to operate when taking, consuming time shorter, shortened the refrigerator open time, less on the temperature impact of other casings.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present application or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, the accompanying drawing the following describes is only some embodiment that record in the application, for those of ordinary skills, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
The sectional structure schematic diagram of the freezing storage box of a kind of stem cell that Fig. 1 provides for the embodiment of the present application;
The surface structure schematic diagram of the freezing storage box of a kind of stem cell that Fig. 2 provides for the embodiment of the present application;
The sectional structure schematic diagram of the freezing storage box of another kind of stem cell that Fig. 3 provides for the embodiment of the present application;
The schematic flow sheet of a kind of stem cell freezing and storing method that Fig. 4 provides for the embodiment of the present application.
Embodiment
In order to make those skilled in the art person understand better the technical scheme in the application, below in conjunction with the accompanying drawing in the embodiment of the present application, technical scheme in the embodiment of the present application is clearly and completely described, obviously, described embodiment is only the application's part embodiment, rather than whole embodiment.Embodiment based in the application, those of ordinary skills are not making the every other embodiment obtaining under creative work prerequisite, all should belong to the scope of the application's protection.
Embodiment mono-:
The sectional structure schematic diagram of the freezing storage box of a kind of stem cell that Fig. 1 provides for the embodiment of the present application.
As shown in Figure 1, in figure, 1 is collection bag, the freezing storage box of this stem cell comprises: casing 2, case lid 3 and a plurality of dividing plate 4, wherein: a plurality of collection bags 1 and a plurality of dividing plate 4 are placed in casing 2, case lid 3 is positioned at the top of casing 2, and be connected with casing 2, case lid 3 is pressed in a plurality of dividing plates 4 and a plurality of collection bag 1 in casing 2.
At the bottom of casing 2 has case and four sidewalls, its top is open end, and top is provided with opening.In order to adapt to the low temperature environment of stem cell when freezing, the material of casing 2 adopts low temperature material, and in the embodiment of the present application, the material of casing 2 is low temperature resistant high molecular polymer, such as low-temperature resistant plastic etc.
As shown in Figure 1, a plurality of collection bags 1 and a plurality of dividing plate 4 are placed in casing 2, and dividing plate 4 is level and smooth plate, and its effect is that a plurality of collection bags 1 are flattened, and avoids occurring between a plurality of collection bags 1 situation of adhesion.In order to guarantee that case lid 3 can continue to exert pressure on dividing plate 4 and collection bag 1, in the embodiment of the present application, the height after a plurality of collection bags 1 and a plurality of dividing plate 4 stack is greater than the degree of depth of casing 2.For each collection bag 1 being pressed into flat, a plurality of collection bags 1 and a plurality of dividing plate 4 are when placing, adopt the mode replacing successively to place, and after placing, top is a dividing plate 4, this is to be collection bag 1 because if top places, when case lid 3 is exerted pressure downwards, may, not in casing 2 inside, so just may there is the problem that collection bag 1 is crushed in the collection bag 1 of top so.In addition, in order to make dividing plate also can adapt to the low temperature environment of stem cell when freezing, dividing plate 4 can adopt and casing 2 same materials, for example low-temperature resistant plastic.
The surface structure schematic diagram of the freezing storage box of a kind of stem cell that Fig. 2 provides for the embodiment of the present application.
In conjunction with Fig. 1, as shown in Figure 2, on casing 2, the outside of relative two sidewalls in position is provided with pressure and regulates fixture, and at the two ends of case lid 3, be provided with a buckle 6, one end of buckle 6 and case lid 3 fix, and can be along rotating up and down with the stiff end of case lid 3, the other end of buckle 6 can regulate fixture to be connected with pressure.It is to coordinate with buckle 6 that pressure regulates the effect of fixture, to case lid 3, provides different directions to point to the pressure of casing 2.It can be row's draw-in groove or row's card column that pressure regulates fixture, and draw-in groove or card column distribution along the vertical direction on the sidewall of casing 2.
In the embodiment of the present application, as depicted in figs. 1 and 2, it is draw-in groove 5 that pressure regulates fixture, and the other end of buckle 6 can be inserted in the draw-in groove 5 on casing 2 sidewalls like this.When buckle 6 is inserted in the draw-in groove 5 of differing heights, can change the pressure size of 3 pairs of collection bags 1 of case lid.In buckle 6 is all inserted into same draw-in groove 5 at every turn, now can also also can regulate the pressure size to collection bag 1 by changing the thickness of dividing plate 4.In addition, if when patient's stem cell sample is less, for example, only have a collection bag 1, now, can in casing, place a plurality of dividing plates 4, can exert pressure to collection bag 1 equally.
Owing to being full of liquid stem cell liquid in collection bag, so when the collection bag between dividing plate is under pressure, can be under the effect of pressure, highly reduce, and and the contact area between dividing plate expands, be that collection bag can be pressed more smoothly, and be forced through in journey at collection bag, can make the stem cell liquid uniform decrease in temperature in collection bag.
For convenient, malaria in casing 2 is discharged, in the embodiment of the present application, as shown in Figure 2, draw-in groove 5 can also be connected with the space in casing 2, and the diameter of draw-in groove 5 is larger, so in use, when collection bag 1 is exerted pressure, can be so that the interior humid air discharge of casing 2.
In addition, as shown in Figure 2, in the embodiment of the present application, two casings 2 can also be linked together, or plural casing 2 is linked together, like this when patient's stem cell sample is more, and a casing 2 is when cannot put down, and patient's stem cell sample can be put in interconnective casing.
For convenient, the interior stem cell model of casing 2 is carried out to mark, as described in Figure 2, on the lateral wall of casing 2, can also be provided with label draw-in groove 7, in Fig. 2,8 is label, so just the label 8 that is filled with patient information can be placed in label draw-in groove 7, conveniently the interior stem cell sample of casing 2 be carried out to mark.
Embodiment bis-:
In Fig. 1 and Fig. 2, case lid 3 is matched the collection bag 1 being placed in casing 2 is exerted pressure with draw-in groove on casing 2 by buckle 6.
In addition,, in other embodiment of the application, as shown in Figure 3, it can also be card column that pressure regulates fixture.
As shown in Figure 3, in figure, 10 is card column, and buckle 6 can fix with card column 10, is connected like this by changing buckle 6 with the card column 10 of differing heights, can adjust equally the pressure size of 3 pairs of collection bags 1 of case lid.
Embodiment tri-:
A kind of stem cell freezing and storing method that Fig. 4 provides for the embodiment of the present application.
As shown in Figure 4, the method comprises the following steps:
S100: a plurality of collection bags and a plurality of dividing plate are alternately placed in the casing with bottom and sidewall successively, and what be positioned at top is dividing plate.
When placing collection bag and dividing plate, in order to make conveniently the dividing plate of top to be exerted pressure, the stack of a plurality of collection bags and a plurality of dividing plates is highly greater than the degree of depth of casing, like this after collection bag and dividing plate placement, the dividing plate of top will be emerged in casing outside, conveniently the dividing plate of top be exerted pressure.
S200: utilize pressure to regulate fixture and buckle to apply to top dividing plate the pressure that direction is pointed to casing, a plurality of collection bags are flattened.
Owing to being full of liquid stem cell liquid in collection bag, thus when the collection bag between dividing plate is under pressure, can be under the effect of pressure, highly reduce, and and dividing plate between contact area expansion, collection bag can be pressed more smoothly.
The above is only the application's preferred embodiment, makes those skilled in the art can understand or realize the application.To the multiple modification of these embodiment, will be apparent to one skilled in the art, General Principle as defined herein can be in the situation that do not depart from the application's spirit or scope, realization in other embodiments.Therefore, the application will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (4)
1. the freezing storage box of stem cell, is placed on the stem cell in collection bag for freezing preservation, it is characterized in that, comprising: casing, case lid and dividing plate, wherein:
Described casing top arranges opening, and on described casing, the outside of relative two sidewalls in position is provided with pressure and regulates fixture;
A plurality of described collection bags and a plurality of described dividing plate are alternately placed in described casing, are highly greater than the degree of depth of described casing after the stack of a plurality of described collection bags and a plurality of described dividing plates, and to be positioned at top be dividing plate;
Described case lid is positioned at described casing top, and described case lid two ends are respectively arranged with buckle, described buckle one end and described case lid fix, the other end regulates fixture to be connected with the pressure on described wall box, described case lid is exerted pressure to the dividing plate of top by described buckle control, and a plurality of described collection bags are driven plain under pressure;
Wherein, described pressure regulates fixture to comprise: row's draw-in groove or row's card column, and a described draw-in groove of row or card column distribution along the vertical direction on the sidewall of described casing;
The material of described casing and dividing plate is low temperature resistant high molecular polymer;
Between at least one sidewall of described casing and described casing, be flexibly connected.
2. storage box according to claim 1, is characterized in that, is provided with the ventilation hole that a plurality of and described case intracorporeal space is connected on described wall box.
3. storage box according to claim 1, is characterized in that, on described casing lateral wall, is provided with label draw-in groove.
4. storage box according to claim 1, is characterized in that, at least one sidewall of described casing is connected by draw-in groove connected mode or the mode that is rotationally connected with described casing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210035051.9A CN102599146B (en) | 2012-02-16 | 2012-02-16 | Stem cell cryopreserving box and cryopreservation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210035051.9A CN102599146B (en) | 2012-02-16 | 2012-02-16 | Stem cell cryopreserving box and cryopreservation method |
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| Publication Number | Publication Date |
|---|---|
| CN102599146A CN102599146A (en) | 2012-07-25 |
| CN102599146B true CN102599146B (en) | 2014-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210035051.9A Expired - Fee Related CN102599146B (en) | 2012-02-16 | 2012-02-16 | Stem cell cryopreserving box and cryopreservation method |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201705652D0 (en) | 2017-04-07 | 2017-05-24 | Asymptote Ltd | Cryopreservation apparatus and methods |
| CN108190215B (en) * | 2018-01-06 | 2019-06-07 | 王云霞 | Cord blood stem cell freezing storage device |
| CN108216931B (en) * | 2018-01-06 | 2020-05-15 | 四川大学华西第二医院 | Low-temperature preservation box for umbilical cord blood stem cells |
| CN107963306B (en) * | 2018-01-06 | 2019-05-31 | 广州赛琅生物技术有限公司 | Stem cell freezing storage device |
| CN108142414B (en) * | 2018-01-06 | 2022-03-15 | 鄂东医疗集团市中心医院 | Stem cell low-temperature storage box |
| CN107934230B (en) * | 2018-01-06 | 2020-04-07 | 东营凤起生物科技发展有限公司 | Cord blood stem cell storage box |
| CN108207933B (en) * | 2018-01-06 | 2019-06-25 | 广州市流式生物科技有限公司 | Cord blood stem cell freezen protective case |
| CN108177878A (en) * | 2018-01-06 | 2018-06-19 | 谷昌德 | Cryopreservation of Human Cord Blood Stem Cells device |
| CN110589190B (en) * | 2019-09-02 | 2020-08-28 | 青岛市中医医院(青岛市海慈医院、青岛市康复医学研究所) | Medical low-temperature cryopreservation equipment |
| CN115176794A (en) * | 2022-05-30 | 2022-10-14 | 五心生物科技(福建)有限公司 | Freezing storage device for low-temperature storage of umbilical cord blood stem cells |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1311989A (en) * | 2001-03-08 | 2001-09-12 | 高大勇 | Cooling preservation method and device for human navel blood stem cell solution |
| CN2466923Y (en) * | 2001-03-08 | 2001-12-26 | 高大勇 | Human body umbilical blood stem cells cryogenic storage apparatus |
| JP2010216667A (en) * | 2009-03-13 | 2010-09-30 | Panasonic Corp | Refrigerator |
| CN202535989U (en) * | 2012-02-16 | 2012-11-21 | 中国人民解放军第三军医大学第二附属医院 | Stem cell freezing preservation box |
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- 2012-02-16 CN CN201210035051.9A patent/CN102599146B/en not_active Expired - Fee Related
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| CN102599146A (en) | 2012-07-25 |
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