Medical low-temperature cryopreservation equipment
Technical Field
The invention relates to the technical field of medical low-temperature preservation, in particular to medical low-temperature cryopreservation equipment.
Background
Cryopreservation refers to a process of freezing biological tissues in a solution added with a cryoprotectant to a certain low temperature and preserving the biological tissues for a long time, and has wide application and requirements in biological research, medical application and other aspects. The freezing process is generally that the biological sample containing the cryoprotectant is frozen to-80 ℃ in a low-temperature refrigerator, then is frozen to-196 ℃ in liquid nitrogen and is stored in the liquid nitrogen for a long time. At present, most containers for low-temperature freezing preservation are box bodies or tank bodies with one ends opened, sample bags are stacked in the box bodies or the tank bodies, so that the sample bags are stacked disorderly, and when the sample bags need to be taken out, the sample bags can only be sequentially picked up and turned over; due to the disordered stacking of the samples, the thickness of each part of the bag body is uneven when the bag body is kept still, the cooling speed of the part with thin thickness is high, the cooling speed of the part with thick thickness is low, and the cooling efficiency is inconsistent; for larger-sized biological tissues, such as cell masses, tissues and the like, the sample is easy to directly contact the wall of the bag body in a standing state and is not protected by the cryoprotectant any more, so that the sample is damaged.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a medical low-temperature cryopreservation device, so that the time for loading and taking a sample bag is saved, and the effect of cryopreservation of other samples in a box is prevented from being influenced by long-time operation.
The purpose of the invention is realized by the following technical scheme:
a medical low-temperature cryopreservation device comprises a base, a supporting spring, a vibration motor and a box body with a front opening, wherein at least one partition plate arranged along the horizontal direction is arranged in the box body, the partition plate divides the inner space of the box body into a plurality of containing cavities which are sequentially arranged along the vertical direction, each containing cavity is internally provided with a lifting plate horizontally arranged, the left side and the right side of the top of the box body are respectively provided with a row of vertical adjusting bolts sequentially arranged along the front-back direction, the number of each row of vertical adjusting bolts is the same as that of the lifting plates, each row of vertical adjusting bolts corresponds to the lifting plates one by one, a top plate of the box body is provided with threaded holes matched with each vertical adjusting bolt, each vertical adjusting bolt is arranged in the corresponding threaded hole in a threaded fit manner through threads, and the lower end of each vertical adjusting bolt is rotatably connected with the corresponding, except the vertical adjusting bolt connected with the lifting plate positioned at the top, other vertical adjusting bolts penetrate through the lifting plate and the partition plate positioned above the lifting plate corresponding to the vertical adjusting bolts, abdicating holes for the vertical adjusting bolts to penetrate are formed in the lifting plate and the partition plate, the lifting plate connected with the vertical adjusting bolts can be driven to lift in the vertical direction in the containing cavity by screwing the vertical adjusting bolts, a box door with a sealed front opening is installed at the front end of the box body, the box body is installed on the base through a plurality of supporting springs, and the vibrating motor is fixedly installed on the box body.
The invention can obviously save the time for loading and taking the samples and avoid the influence on the freezing and storing effect of other samples in the box due to long-time operation. When a sample is stored, the lifting plate is lifted to the highest point in advance, then the sample is placed above the partition plate or the tray, the box door is closed randomly, and then the lifting plates are pressed on the upper part of the sample by screwing the vertical adjusting bolt to evenly flatten the sample; when a sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed, so that the box door can be quickly closed when the sample is taken and put.
Preferably, two vertical adjusting bolts connected to the same lifting plate are arranged approximately symmetrically about the center of the lifting plate, the central symmetry does not need to be very precise, but only needs to be approximately central symmetry, so as to avoid the lifting plate from being stressed unevenly and generating blockage, and the design is also preferred and not necessary. In fact, the lifting driving force of the connecting plate is not large, the connecting plate can work smoothly even if the connecting plate is clamped, and the structure is only an optimized design.
A sample is placed in each containing cavity and comprises a bag body, a cryoprotectant filled in the bag body and biological tissues immersed in the cryoprotectant.
Preferably, the left side and the right side of the partition board are provided with a convex edge, and the convex edges are positioned on the inner sides of the vertical adjusting bolts and used for preventing a sample positioned on the partition board from contacting the vertical adjusting bolts.
Preferably, the bottom of each accommodating cavity is provided with a roller with an axis arranged along the horizontal direction and perpendicular to the front-back direction. The roller is used for reducing the contact area of the sample and the clapboard, and the sample is taken out through the rotation of the roller. The sample damage caused by excessive contact and friction between the sample and the partition plate is avoided, and the sample is protected.
Preferably, the bottom of each accommodating cavity is provided with a sliding rail extending along the front-back direction, and a tray for accommodating samples is slidably mounted on the sliding rail.
When the sample pressing device is used, the vertical adjusting bolts are screwed, the lifting plates in the containing cavities are lifted to the highest point, then the sample is placed above the partition plate or the tray, the box door is closed, and the lifting plates are pressed on the upper part of the sample to evenly flatten the sample by screwing the vertical adjusting bolts;
the vibration motor is kept working in the freezing process, the vibration motor drives the box body to vibrate, and the vibration motor stops working after the freezing link is finished.
When the sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed immediately.
Preferably, the door is a transparent door made of transparent materials. Therefore, when the box door is not opened, the containing cavity in which each sample is located can be seen, the lifting plate corresponding to the upper part of the sample is lifted by operating the corresponding vertical adjusting bolt, the sample can be quickly taken out after the box door is opened, and the box door is quickly closed before the box door is opened.
The invention has the following advantages:
the invention can obviously save the time for loading and taking the samples and avoid the influence on the freezing and storing effect of other samples in the box due to long-time operation. When a sample is stored, the lifting plate is lifted to the highest point in advance, then the sample is placed above the partition plate or the tray, the box door is closed randomly, and then the lifting plates are pressed on the upper part of the sample by screwing the vertical adjusting bolt to evenly flatten the sample; when a sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed, so that the box door can be quickly closed when the sample is taken and put.
Keep vibrating motor work at freezing in-process, vibrating motor drives the box vibration, and after freezing link, vibrating motor stop work makes the sample be in the suspended state all the time, can effectively avoid sample direct contact bag body wall.
The height of the lifting plate of each accommodating cavity is independently adjusted, so that the lifting plate can be suitable for samples of different specifications.
Drawings
Fig. 1 is a main sectional structural view of the lifter plate of the present invention at the highest point.
FIG. 2 is a schematic view of the main cross-sectional structure of the lifting plate of the present invention when the lifting plate presses a sample.
FIG. 3 is a schematic side sectional view of the lifter plate of the present invention in a position for pressing a sample.
Fig. 4 is a main sectional structural view of the present invention when a tray structure is provided.
1-base, 2-supporting spring, 3-vibrating motor, 41-box, 42-partition board, 43-lifting board, 44-vertical adjusting bolt, 46-convex edge, 47-roller, 48-slide rail and 49-tray.
Detailed Description
The invention is further described with reference to the accompanying drawings in which:
as shown in fig. 1, 2 and 3, a medical low-temperature cryopreservation device comprises a base 1, a support spring 2, a vibration motor 3 and a box 41 with a front opening, wherein at least one partition plate 42 arranged along the horizontal direction is arranged in the box 41, the partition plate 42 divides the inner space of the box 41 into a plurality of accommodating cavities sequentially arranged along the vertical direction, a lifting plate 43 arranged horizontally is arranged in each accommodating cavity, a row of vertical adjusting bolts 44 sequentially arranged along the front-back direction is arranged on the left side and the right side of the top of the box 41, the number of each row of vertical adjusting bolts 44 is the same as that of the lifting plate 43, the vertical adjusting bolts 44 of each row correspond to the lifting plate 43 one by one, threaded holes matched with the vertical adjusting bolts 44 are arranged on the top plate of the box 41, the vertical adjusting bolts 44 are arranged in the corresponding threaded holes in a threaded fit manner, the lower end of each vertical adjusting bolt 44 is rotatably connected with the corresponding lifting plate 43, except the vertical adjusting bolt 44 connected with the lifting plate 43 positioned at the uppermost part, the other vertical adjusting bolts 44 pass through the lifting plate 43 and the partition plate 42 positioned above the corresponding lifting plate 43, the lifting plate 43 and the partition plate 42 are provided with abdicating holes for the vertical adjusting bolts 44 to pass through, the lifting plate 43 connected with the vertical adjusting bolts 44 can be driven to lift in the vertical direction in the accommodating cavity by screwing the vertical adjusting bolts 44, the front end of the box body 41 is provided with a box door with a sealed front opening, the box body 41 is arranged on the base 1 through a plurality of supporting springs 2, and the vibration motor 3 is fixedly arranged on the box body 41.
As shown in fig. 1-4, in the operation method of the low-temperature cryopreservation device of the present invention, the vertical adjusting bolt 44 corresponding to the lifting plate 43 in the containing cavity where no sample is stored is screwed, so as to lift the lifting plate 43 in the containing cavity where no sample is stored to the highest point, then the door of the chamber is opened to place the sample above the partition plate 42 or the tray 49, and the door of the chamber is closed; screwing up vertical adjusting bolts 44 corresponding to the lifting plates 43 placed in the accommodating cavities of the samples in the step, and pressing the lifting plates 43 on the upper parts of the samples to uniformly flatten the samples; the vibration motor 3 is kept working in the freezing process, the vibration motor 3 drives the box body 41 to vibrate, and after the freezing link is finished, the vibration motor 3 stops working; when the sample needs to be taken out, the vertical adjusting bolt 44 corresponding to the lifting plate 43 covering the sample is firstly screwed, so that the lifting plate 43 is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed. The method of operation of the cryopreservation apparatus further comprises the step of deriving the sample by rotation of the roller 47. The method of operation of the cryopreservation apparatus further comprises the step of taking out the sample by sliding the tray 49 along the slide rails 48.
Preferably, the two vertical adjusting bolts 44 connected to the same lifting plate 43 are arranged substantially symmetrically about the center of the lifting plate 43, and the central symmetry need not be precise, but only substantially symmetrical, so as to avoid the lifting plate 43 from being stressed unevenly and causing jamming, and this is also a preferred design and is not necessary. In fact, the lifting driving force of the connecting plate is not large, the connecting plate can work smoothly even if the connecting plate is clamped, and the structure is only an optimized design.
A sample is placed in each containing cavity and comprises a bag body, a cryoprotectant filled in the bag body and biological tissues immersed in the cryoprotectant.
Preferably, the partition plate 42 is provided with a ridge 46 on both left and right sides, and the ridge 46 is located inside the vertical adjusting bolt 44 for preventing the sample on the partition plate 42 from contacting the vertical adjusting bolt 44.
Preferably, as shown in fig. 1, the bottom of each accommodating chamber is provided with a roller 47 having an axis arranged in a horizontal direction and perpendicular to the front-rear direction. The roller 47 serves to reduce the contact area of the sample with the partition 42 and take out the sample by the rotation of the roller 47. The sample is prevented from being damaged due to excessive contact and friction between the sample and the partition plate 42, and the sample is protected.
Preferably, as shown in fig. 4, the bottom of each accommodating cavity is provided with a slide rail 48 extending in the front-back direction, and a tray 49 for accommodating samples is slidably mounted on the slide rail 48.
When the sample pressing device is used, the vertical adjusting bolts 44 are screwed, as shown in fig. 1, the lifting plates 43 in the containing cavities are lifted to the highest point, then the sample is placed above the partition plate 42 or the tray 49, the box door is closed, as shown in fig. 2 and 3, the vertical adjusting bolts 44 are screwed to press the lifting plates 43 on the upper portion of the sample, and the sample is uniformly pressed and flattened;
the vibration motor 3 is kept working in the freezing process, the vibration motor 3 drives the box body 41 to vibrate, and after the freezing link is finished, the vibration motor 3 stops working.
When the sample needs to be taken out, the vertical adjusting bolt 44 corresponding to the lifting plate 43 covering the sample is firstly screwed, so that the lifting plate 43 is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed.
Preferably, the door is a transparent door made of transparent materials. Therefore, the accommodating cavity in which each sample is positioned can be seen when the box door is not opened. Before the door of the box is opened, the lifting plate 43 corresponding to the upper part of the sample can be lifted by operating the corresponding vertical adjusting bolt 44, the sample can be taken out quickly after the door of the box is opened, and the door of the box can be closed quickly.
The invention can obviously save the time for loading and taking the samples and avoid the influence on the freezing and storing effect of other samples in the box due to long-time operation. When a sample is stored, the lifting plate is lifted to the highest point in advance, then the sample is placed above the partition plate or the tray, the box door is closed randomly, and then the lifting plates are pressed on the upper part of the sample by screwing the vertical adjusting bolt to evenly flatten the sample; when a sample needs to be taken out, the vertical adjusting bolt corresponding to the lifting plate covering the sample is firstly screwed, so that the lifting plate is lifted to the highest point to be separated from the sample, then the box door is opened to take out the sample, and then the box door is closed, so that the box door can be quickly closed when the sample is taken and put.
Keep vibrating motor work at freezing in-process, vibrating motor drives the box vibration, and after freezing link, vibrating motor stop work makes the sample be in the suspended state all the time, can effectively avoid sample direct contact bag body wall. The height of the lifting plate of each accommodating cavity is independently adjusted, so that the lifting plate can be suitable for samples of different specifications.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.