CN1311989A - Cooling preservation method and device for human navel blood stem cell solution - Google Patents
Cooling preservation method and device for human navel blood stem cell solution Download PDFInfo
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- CN1311989A CN1311989A CN 01108121 CN01108121A CN1311989A CN 1311989 A CN1311989 A CN 1311989A CN 01108121 CN01108121 CN 01108121 CN 01108121 A CN01108121 A CN 01108121A CN 1311989 A CN1311989 A CN 1311989A
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Abstract
The present invention relates to a hypothermal storage method of human cord blood dry cell and its equipment, including the process of existent technology, such as separating dry cell from fresh human cord blood, adding low-temp. protection liquor to make treatment and placing the treated cord blood dry cell into special-purpose sealed cell bag, the invented next operation process includes following steps: (1) placing the cell bag into internal box of special-purpose storage box; (2) placing the internal box on the support being in external box of the storage box, and making the distance of every external surface of the internal box and internal wall surface of box cavity identical each other; (3). placing the storage box in constant temp. (-50 deg.c to -80 deg.C) low-temp. environment for 2-3 hr.; and (4). taking out storage box and placing the whole storage box in deep low-temp. liquid nitrogen tank. It possesses the advantages of small investment and convenient operation, etc.
Description
The present invention relates to the cooling preservation method and the device of human navel blood stem cell.
Human navel blood stem cell is medically having many special purposes.How to preserve in a long time is a very complicated problems.General process is, from bleeding of the umbilicus, isolate stem cell after, progressively add hypothermic protective solution again, cool to about-80 ℃ with certain speed (mostly being controlled in 1-3 ℃/minute) at last, place the profound hypothermia liquid nitrogen container to preserve.Temperature-fall period of the prior art is realized by program frozen cooling system.Concrete is to utilize solenoid control liquid nitrogen spray flow, is aided with electrical heating again, reaches the purpose of control cooling rate.But usually can run into unmanageable problem in actual the use, make the navel blood stem cell can be dead because of improper property rewarming.For example (1) magnetic valve damages suddenly in temperature-fall period, when (2) running into the unexpected loss of power accident, liquid nitrogen also can stop to spray immediately, (3) when cooling to storage temperature, need to take out the stem cell bag, put it in the liquid nitrogen container that (in the middle of this process, navel blood stem cell (1-2 minute) for some time is the situation of exposing in normal temperature environment.Because stem cell seldom, thermal inertia is little, and these three incidents can make navel blood stem cell dead because of the non-controlling rewarming.Comprehensive above 3 as can be seen, fundamentally there are hidden danger of quality in present program frozen cooling system and operating process thereof in temperature-fall period.Navel blood stem cell is preserved failure, can't estimate in loss medically, and it tends to directly have influence on the treatment chance of a patient and even one family.In addition, the capacity of described program frozen cooling system is very little, the two parts of bleedings of the umbilicus of generally can only lowering the temperature simultaneously, so this system need consume a large amount of liquid nitrogen every day.And need be through personnel's substantial length of operation of special training, day-to-day operation expense height.The objective of the invention is to:, provide a kind of store method and device thereof that can make the human navel blood stem cell that is contained in the cell bags with the proper speed cooling at deficiency of the prior art.
Purpose of the present invention is realized by following mode.
The cooling preservation method of human navel blood stem cell of the present invention comprises the special-purpose encapsulated cell bag of packing into after fresh human navel blood is isolated stem cell and added hypothermic protective solution in the prior art.It is characterized in that operating process after this is: (1) is placed on cell bags in the inner box of special-purpose conserving case, and each surface of bag is all contacted with the internal face in box chamber; (2) inner box is put on the support in the outer box of conserving case, makes the distance of internal face in each outer surface of inner box and box chamber impartial; (3) again conserving case is put into constant temperature in-50 ℃ to-80 ℃ low temperature environment 2-3 hour, for example-80 in ℃ low temperature refrigerator; (4) take out conserving case, integral body places the profound hypothermia liquid nitrogen container.Described special-purpose conserving case comprises inner box and the outer box that is made of metal material, and it is characterized in that: the shape in its inner box chamber and the shape of cell bags match; The inner bottom surface of outer box is provided with support, inner box is placed on this support, distance between each outside wall surface of inner box and the internal face of outer box is impartial, be preferably 0.2-0.5cm, can place two or more inner boxs in the inner chamber of an outer box, the spacing of inner box and inner box is equal to the distance of its box wall and outer box internal face; Described outer box is the metal material configuration example of 20-40w/mK such as stainless steel etc. by thermal conductivity factor, and the metal material of described inner box is that thermal conductivity factor is the metal of 100-200w/mK, for example aluminium etc.
The cooling preservation method of human navel blood stem cell of the present invention uses the combined type inner and outer box of ad hoc structure, and puts it in the low temperature environment.The control song of its cooling rate is realized the selection of the material of box body and planform thereof.Because have certain the air gap between the inside and outside box, thermal resistance is very big.Add the accumulation of heat effect of outer box material, through suitably design, the cooling rate of cell can be controlled in the scope of requirement in the inner box.According to the calculating of mathematical Model of Heat Transfer, when the thermal conductivity factor of cell material, spacing between the inner and outer box wall are the described setting value of wood invention, the cooling rate of described cell solution will be controlled at about 1-3 ℃/minute, satisfy instructions for use.
Use cooling preservation method of the present invention to the human navel blood stem cell solution preservation of lowering the temperature, can overcome the defective that causes cell death in the prior art because of improper property rewarming.Have small investment, advantage such as easy to operate.The combined type inner and outer box of ad hoc structure of the present invention, simple in structure, material therefor is the universal metal material, cost of manufacture is low.Program frozen cooling system can only preserve two parts of bleedings of the umbilicus at every turn in the prior art, and this kind method can be handled many parts simultaneously.When using low temperature refrigerator as low temperature environment, in temperature-fall period, can open the low temperature refrigerator door at any time, very convenient in the operation.In the use, need not special operating personnel, also without substantial length of operation.
Below by embodiment and accompanying drawing the present invention is done to describe further.
Fig. 1 is a kind of example structure schematic diagram of combination conserving case of the present invention.
Fig. 2 is the A-A cutaway view of Fig. 1.
Referring to Fig. 1, (1) is outer box, and (2) are inner box.From integral body, outer box is a rectangle, evenly places 3 inner boxs in each outer box.Open for convenient, the connection of inner and outer box is a drawer type.
Referring to Fig. 2, (3) are the supports that places outer box, are used to place inner box.(4) be the stem cell solution bag, it is placed in the inner box, and each surface of this bag all contacts with the internal face in box chamber.Distance between each outside wall surface of inner box and the internal face of outer box is impartial, is guaranteed by the support between them.(5) be that mouth is put in the extraction of support and inner box.
The embodiment of the cooling preservation method of human navel blood below is provided.
1) isolates stem cell and add the special-purpose encapsulated cell bag of packing into after hypothermic protective solution is handled from fresh human navel blood; 2) cell bags is placed in the inner box of special-purpose conserving case, each surface of bag is all contacted with the internal face in box chamber; 3) inner box is put on the support in the outer box of conserving case, makes the distance of internal face in each outer surface of inner box and box chamber impartial; 4) again conserving case is put into constant temperature in-80 ℃ of low temperature refrigerators 2-3 hour; 5) take out conserving case, integral body places the profound hypothermia liquid nitrogen container.Described outer box is a flat case, and its cross dimensions is: width 19.5 ± 2cm, height 1.5 ± 0.5cm, wall thickness 0.76mm ± 0.2; Its material is a stainless steel, and thermal conductivity factor is that 20-40w/mK, specific heat are 0.4 ± 0.2kJ/kgK.Box inside is put 4 inner boxs side by side outside, each box length * wide * height=(15.2 ± 2) * (18.7 ± 2) * (1.0 ± 0.5) cm, its inner box material is an aluminium, its thermal conductivity factor is 100-200w/mK, specific heat is 0.92 ± 0.2kJ/kgK, and the space of 0.25 ± 0.5cm is arranged between the inner and outer box.
Claims (6)
1, the cooling preservation method of human navel blood stem cell solution, comprise the special-purpose encapsulated cell bag of after fresh human navel blood is isolated stem cell and added the hypothermic protective solution processing, packing in the prior art, it is characterized in that: operating process after this is: (1) is placed on cell bags in the inner box of special-purpose conserving case, and each surface of bag is all contacted with the internal face in box chamber; (2) inner box is put on the support in the outer box of conserving case, makes the distance of internal face in each outer surface of inner box and box chamber impartial; (3) again conserving case is put into constant temperature in-50 ℃ to-80 ℃ low temperature environment 2-3 hour; (4) take out conserving case, integral body places the profound hypothermia liquid nitrogen container.
2, the special-purpose conserving case that uses in the cooling preservation method of human navel blood stem cell solution according to claim 1, it comprises inner box and the outer box that is made of metal material, it is characterized in that: the shape in its inner box chamber and the shape of cell bags match; The inner bottom surface of outer box is provided with support, and inner box is placed on this support, and the distance between each outside wall surface of inner box and the internal face of outer box is impartial.
3, special-purpose conserving case according to claim 2 is characterized in that: the distance between each outside wall surface of its inner box and the internal face of outer box is preferably 0.2-0.5cm.
4, special-purpose conserving case according to claim 2 is characterized in that: can place two or more inner boxs in the inner chamber of an outer box, the spacing of inner box and inner box is equal to the distance of its box wall and outer box internal face.
5, special-purpose conserving case according to claim 2 is characterized in that: described outer box is that the metal material of 20-40w/mK constitutes by thermal conductivity factor.
6, special-purpose conserving case according to claim 2 is characterized in that: the metal material of described inner box is that thermal conductivity factor is the metal of 100-200w/mK.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN 01108121 CN1311989A (en) | 2001-03-08 | 2001-03-08 | Cooling preservation method and device for human navel blood stem cell solution |
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CN 01108121 CN1311989A (en) | 2001-03-08 | 2001-03-08 | Cooling preservation method and device for human navel blood stem cell solution |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101462632B (en) * | 2007-12-20 | 2011-05-18 | 生宝生物科技股份有限公司 | Storing apparatus for storing and storage system thereof |
CN102160546A (en) * | 2010-02-24 | 2011-08-24 | 瓦埃尔科有限责任公司 | Systems and methods for cryopreservation of cells |
CN102599146A (en) * | 2012-02-16 | 2012-07-25 | 中国人民解放军第三军医大学第二附属医院 | Stem cell cryopreserving box and cryopreservation method |
-
2001
- 2001-03-08 CN CN 01108121 patent/CN1311989A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101462632B (en) * | 2007-12-20 | 2011-05-18 | 生宝生物科技股份有限公司 | Storing apparatus for storing and storage system thereof |
CN102160546A (en) * | 2010-02-24 | 2011-08-24 | 瓦埃尔科有限责任公司 | Systems and methods for cryopreservation of cells |
CN102160546B (en) * | 2010-02-24 | 2016-01-20 | 库克通用生物科技有限责任公司 | The system and method for Cell Cryopreservation |
CN102599146A (en) * | 2012-02-16 | 2012-07-25 | 中国人民解放军第三军医大学第二附属医院 | Stem cell cryopreserving box and cryopreservation method |
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