CN115176794A - Freezing storage device for low-temperature storage of umbilical cord blood stem cells - Google Patents
Freezing storage device for low-temperature storage of umbilical cord blood stem cells Download PDFInfo
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- CN115176794A CN115176794A CN202210586694.6A CN202210586694A CN115176794A CN 115176794 A CN115176794 A CN 115176794A CN 202210586694 A CN202210586694 A CN 202210586694A CN 115176794 A CN115176794 A CN 115176794A
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 51
- 210000004700 fetal blood Anatomy 0.000 title claims abstract description 50
- 238000003860 storage Methods 0.000 title claims abstract description 27
- 238000007710 freezing Methods 0.000 title claims description 18
- 230000008014 freezing Effects 0.000 title claims description 18
- 238000007789 sealing Methods 0.000 claims abstract description 106
- 238000005138 cryopreservation Methods 0.000 claims abstract description 37
- 238000005192 partition Methods 0.000 claims abstract description 36
- 238000001125 extrusion Methods 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 4
- 238000000151 deposition Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mechanical Engineering (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Medical Preparation Storing Or Oral Administration Devices (AREA)
Abstract
The invention discloses a cryopreservation device for low-temperature storage of umbilical cord blood stem cells, which comprises a cryopreservation device main body, a bottom partition plate, a middle partition plate and a top partition plate, wherein a bottom sealing groove is formed in the center of the bottom partition plate, a bottom sealing block is inserted in the bottom sealing groove, a middle sealing groove is formed in the center of the middle partition plate, a middle sealing block is inserted in the middle sealing groove, a top sealing groove is formed in the center of the top partition plate, and a top sealing block is inserted in the top sealing groove. According to the invention, the bottom sealing block, the middle sealing block and the top sealing block are respectively blocked in the bottom sealing groove, the middle sealing groove and the top sealing groove, and the interior of the main body of the cryopreservation device is divided into four parts by virtue of the top partition plate, the middle partition plate and the bottom partition plate, so that all umbilical cord blood stem cells in the main body of the cryopreservation device can be prevented from being simultaneously polluted, and the storage quality of the umbilical cord blood stem cells is ensured.
Description
Technical Field
The invention relates to the technical field of a cryopreservation device for cord blood stem cells, in particular to a cryopreservation device for low-temperature storage of cord blood stem cells.
Background
The cord blood contains a large amount of hematopoietic stem cells and a plurality of other stem cells, the cord blood stem cells are obtained by utilizing various stem cells in the cord blood to carry out experiments in a clinical laboratory, which is favorable for promoting the repair work of body tissues and damaged organs, so that the storage and clinical application work of the cord blood stem cells are facilitated, the cord blood stem cells which are enriched in various stem cells are obtained by removing red blood cells and blood plasma as far as possible through separation and preparation, and the enriched cord blood stem cells are stored in a liquid nitrogen tank with a deep low temperature and are in a dormant state, so that the cord blood stem cells can be conveniently used when clinical treatment is needed.
Cord blood stem cells are usually stored in a liquid nitrogen tank at a deep low temperature, cord blood stem cells need to be taken from the liquid nitrogen tank at irregular intervals in the long-term storage process, in the process of taking the cord blood stem cells, external bacteria easily enter the inside of the liquid oxygen device, and although most of bacteria are not easy to survive in a low-temperature environment, the quality of the cord blood stem cells inside the device can still be influenced by a small part of bacteria.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a cryopreservation device for storing umbilical cord blood stem cells at low temperature, and the technical problems to be solved by the invention are as follows: in the process of taking umbilical cord blood stem cells, external bacteria easily enter the liquid oxygen device, so that the quality of the umbilical cord blood stem cells in the device is influenced.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a freeze and deposit device for cord blood stem cell low temperature is stored, deposits the device main part including freezing, the top of freezing the device main part is passed through bolt fixedly connected with device closing cap, freeze four outer takeovers of one side fixedly connected with of depositing the device main part, the inboard on-off valve of bolt fixedly connected with is passed through to one side of outer takeover, freeze the bottom inner wall fixedly connected with bottom baffle of depositing the device main part, the bottom seal groove has been seted up to the inside at bottom baffle center, the inside of bottom seal groove is pegged graft and is had the bottom seal piece, the outer wall of bottom seal piece is laminated with the inner wall of bottom seal groove mutually, freeze the middle part inner wall fixedly connected with middle part baffle of depositing the device main part, the middle part seal groove has been seted up to the inside at middle part baffle center, the inside of middle part seal groove is pegged graft and is had the middle part seal piece, the outer wall of middle part seal piece is laminated with the inner wall of middle part seal groove mutually, the top inner wall fixedly connected with top baffle of freezing the device main part of depositing the device main part baffle center, the inside of top seal piece is pegged graft and is connected with the top seal piece, the outer wall of top seal piece is laminated with the inner wall of top seal piece and the same pole setting of top seal piece, the bottom seal piece.
In a preferred embodiment, one side of the inner on-off valve is fixedly connected with an adapter tube through a bolt, one side of the adapter tube is fixedly connected with an outer on-off valve through a bolt, one side of the outer on-off valve is fixedly connected with a liquid discharging tube through a bolt, and by using the outer on-off valve and the inner on-off valve, the probability of pollution of umbilical cord blood stem cells in a freezing device body can be reduced, and the storage time of the umbilical cord blood stem cells is prolonged.
In a preferred embodiment, a guide disc is fixedly connected to the inner wall of the bottom of the cryopreservation device body, the section of the guide disc is triangular, and umbilical cord blood stem cells far away from one side of the extension tube can flow into the bottom extension tube by fixedly connecting the guide disc with the triangular section to the inner wall of the bottom of the cryopreservation device body.
In a preferred embodiment, the bottom seal block has a top surface circle diameter smaller than a bottom surface circle diameter of the middle seal block, the middle seal block has a top surface circle diameter smaller than a bottom surface circle diameter of the top seal block, and the bottom seal block is conveniently connected inside the bottom seal groove through the top seal groove and the middle seal groove by making the top surface circle diameter of the bottom seal block smaller than the bottom surface circle diameter of the middle seal block and making the top surface circle diameter of the middle seal block smaller than the bottom surface circle diameter of the top seal block.
In a preferred embodiment, the top end of the top sealing block is fixedly connected with a top guide circular truncated cone, the top end of the middle sealing block is fixedly connected with a middle guide circular truncated cone, the top end of the bottom sealing block is fixedly connected with a bottom guide circular truncated cone, and the connection strength of the top sealing block, the middle sealing block and the bottom sealing block with the main control vertical rod is increased conveniently by using the top guide circular truncated cone, the middle guide circular truncated cone and the bottom guide circular truncated cone.
In a preferred embodiment, the top of always controlling the pole setting is provided with the extrusion sleeve, the positioning disk has been cup jointed to the inside of extrusion sleeve, the bottom fixedly connected with extrusion spring of positioning disk, the bottom of extrusion spring carries out fixed connection with the bottom inner wall of extrusion sleeve, the top fixedly connected with sealed dish of positioning disk, sealed dish swing joint is in the inside of device closing cap, through making extrusion spring coupling at positioning disk and extrusion sleeve, is convenient for extrude always controlling the pole setting and prevent that the threaded rod is connected loosely with the device closing cap to the bottom.
In a preferred embodiment, the top fixedly connected with of sealed dish rotates the connecting seat, the inside swing joint that rotates the connecting seat has spacing dish, the top fixedly connected with threaded rod of spacing dish, threaded rod threaded connection is in the inside of device closing cap, through the use to threaded rod, spacing dish and rotation connecting seat, is convenient for through the threaded connection condition control sealed dish of adjusting threaded rod and control hand wheel to bottom motion.
In a preferred embodiment, a control hand wheel is fixedly connected to the top end of the threaded rod, the control hand wheel is located at the top of the device cover, and the control hand wheel is used for conveniently controlling the threaded rod to rotate at the top of the device cover.
In a preferred embodiment, the bottom end of the extrusion sleeve is provided with a vertical rod slot, the top of the master control vertical rod is inserted into the vertical rod slot, and the extrusion sleeve and the master control vertical rod can be conveniently connected by using the opposite rod slot.
In a preferred embodiment, the cross-section of outside on-off valve, the bottom seal groove, the middle part seal piece, top seal piece and top seal groove is trapezoidal, through setting the cross-section of outside on-off valve, the bottom seal groove, the middle part seal piece, top seal piece and top seal groove trapezoidal, utilize always to control the pole setting with top seal piece, the inside at the top seal groove is pegged graft respectively to middle part seal piece and bottom seal piece, can prevent to always control the pole setting and drop to the bottom, and at the in-process of always controlling the pole setting of pushing down, can guarantee the leakproofness of top seal piece, middle part seal piece and bottom seal piece.
The invention has the technical effects and advantages that:
1. according to the invention, the master control upright rod penetrates through the top sealing groove, the middle sealing groove and the inside of the bottom sealing groove, so that the bottom sealing block, the middle sealing block and the top sealing block are respectively blocked in the bottom sealing groove, the middle sealing groove and the inside of the top sealing groove, the inside of the cryopreservation device main body can be divided into four parts by means of the top partition plate, the middle partition plate and the bottom partition plate, and all umbilical cord blood stem cells in the cryopreservation device main body are prevented from being simultaneously polluted.
2. The umbilical cord blood stem cells of the four parts in the freezing storage device main body are discharged from the inside of the external connection pipe by utilizing the four inner on-off valves, so that the umbilical cord blood stem cells in the freezing storage device main body can be taken and used successively, and the umbilical cord blood stem cells in the freezing storage device main body are prevented from being polluted simultaneously.
3. According to the invention, the threaded connection condition of the hand wheel adjusting threaded rod and the device sealing cover is controlled by rotating the top of the device sealing cover, the guide disc connected with the sealing disc is pushed towards the bottom, so that the extrusion sleeve sleeved outside the guide disc pushes the master control vertical rod to move towards the bottom by utilizing the extrusion sleeve, the outer walls of the bottom sealing block, the middle sealing block and the top sealing block are respectively attached to the inner walls of the bottom sealing groove, the middle sealing groove and the top sealing groove, and the four inner cavities separated by the top partition plate, the middle partition plate and the bottom partition plate are prevented from being mutually communicated.
Drawings
FIG. 1 is a front view of the present invention;
FIG. 2 is an enlarged view of portion A of FIG. 1;
FIG. 3 is a cross-sectional view of the cryopreservation device body of the present invention;
FIG. 4 is an enlarged view of the portion B of FIG. 3 according to the present invention;
FIG. 5 is an enlarged view of the portion C of FIG. 3 according to the present invention;
FIG. 6 is an enlarged view of portion D of FIG. 3 according to the present invention;
FIG. 7 is a front view of a master control upright of the present invention;
fig. 8 is a schematic cross-sectional view of a closure of the device of the present invention.
The reference signs are: 1. a control hand wheel; 2. a device cover; 3. freezing and storing the device main body; 4. an external connection pipe; 5. a master control upright rod; 6. a top partition; 7. a middle partition plate; 8. a bottom partition; 9. a guide plate; 10. the top part guides the round platform; 11. a top sealing block; 12. the middle part of the guide circular table; 13. a middle seal block; 14. the bottom part is guided to the round table; 15. a bottom sealing block; 16. an inner side on-off valve; 17. a transfer tube; 18. an outer on-off valve; 19. a liquid discharging pipe; 20. a top seal groove; 21. a middle seal groove; 22. a bottom seal groove; 23. a threaded rod; 24. a limiting disc; 25. rotating the connecting seat; 26. sealing the disc; 27. a guide plate; 28. a vertical rod slot; 29. a compression spring; 30. the sleeve is squeezed.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to the attached drawings 3-7 in the specification, the cryopreservation device for storing umbilical cord blood stem cells at low temperature comprises a cryopreservation device main body 3, wherein a guide disc 9 is fixedly connected to the inner wall of the bottom of the cryopreservation device main body 3, the section of the guide disc 9 is triangular, umbilical cord blood stem cells far away from one side of an external connecting pipe 4 can flow into the internal part of the external connecting pipe 4 by fixedly connecting the guide disc 9 with the inner wall of the bottom of the cryopreservation device main body 3, a bottom partition plate 8 is fixedly connected to the inner wall of the bottom of the cryopreservation device main body 3, a bottom sealing groove 22 is formed in the center of the bottom partition plate 8, a bottom sealing block 15 is inserted in the bottom sealing groove 22, the outer wall of the bottom sealing block 15 is attached to the inner wall of the bottom sealing groove 22, a middle partition plate 7 is fixedly connected to the inner wall of the middle part of the cryopreservation device main body 3, and a middle sealing groove 21 is formed in the center of the middle partition plate 7, a middle sealing block 13 is inserted in the middle sealing groove 21, the outer wall of the middle sealing block 13 is attached to the inner wall of the middle sealing groove 21, a top partition plate 6 is fixedly connected to the inner wall of the top of the cryopreservation device main body 3, a top sealing groove 20 is formed in the center of the top partition plate 6, a top sealing block 11 is inserted in the top sealing groove 20, the outer wall of the top sealing block 11 is attached to the inner wall of the top sealing groove 20, the interiors of the top sealing block 11, the middle sealing block 13 and the bottom sealing block 15 are fixedly connected with a same general control upright rod 5, the top end of the top sealing block 11 is fixedly connected with a top guide circular truncated cone 10, the top end of the middle sealing block 13 is fixedly connected with a middle guide circular truncated cone 12, the top end of the bottom sealing block 15 is fixedly connected with a bottom guide circular truncated cone 14, and the top guide circular truncated cone 10, the middle guide circular truncated cone 12 and the bottom guide circular truncated cone 14 are used, it is convenient for increase top seal block 11, middle seal block 13 and bottom seal block 15 and the joint strength of total accuse pole setting 5, the top surface circle diameter of bottom seal block 15 is less than the bottom surface circle diameter of middle seal block 13, the top surface circle diameter of middle seal block 13 is less than the bottom surface circle diameter of top seal block 11, through making the top surface circle diameter of bottom seal block 15 less than the bottom surface circle diameter of middle seal block 13 and making the top surface circle diameter of middle seal block 13 less than the bottom surface circle diameter of top seal block 11, it is convenient for bottom seal block 15 to pass top seal groove 20 and middle seal groove 21 and connect the inside at bottom seal groove 22, outside on-off valve 18, bottom seal groove 22, middle seal block 21, middle seal block 13, the cross-section of top seal block 11 and top seal groove 20 sets up to be trapezoidal, through with outside on-off valve 18, bottom seal groove 22, middle part 21, middle seal block 13, the cross-section of top seal block 11 and top seal groove 20 sets up to be trapezoidal, utilize top seal block 11, middle seal block 13 and bottom seal block 15 to insert in top seal groove 20 respectively, the inside of top seal groove can prevent bottom seal block 5 and bottom seal groove from dropping, the bottom seal block 15 and the bottom seal groove can prevent the bottom seal block from pressing down in the process of top seal block.
The implementation scenario is specifically as follows:
in the using process of the invention, the master control upright rod 5 penetrates through the inside of the top sealing groove 20, the middle sealing groove 21 and the bottom sealing groove 22, so that the bottom sealing block 15 is blocked inside the bottom sealing groove 22, the middle sealing block 13 is blocked inside the middle sealing groove 21, and the top sealing block 11 is blocked inside the top sealing groove 20, the inside of the cryopreservation device main body 3 can be divided into four parts by the top partition 6, the middle partition 7 and the bottom partition 8, all umbilical cord blood stem cells in the cryopreservation device main body 3 can be prevented from being simultaneously polluted, and the storage quality of the umbilical cord blood stem cells is ensured.
Referring to the attached drawings 1 and 2 of the specification, the cryopreservation device for low-temperature storage of umbilical cord blood stem cells comprises a cryopreservation device body 3, wherein four external connecting pipes 4 are fixedly connected to one side of the cryopreservation device body 3, an inner side on-off valve 16 is fixedly connected to one side of each external connecting pipe 4 through a bolt, an adapter pipe 17 is fixedly connected to one side of each inner side on-off valve 16 through a bolt, an outer side on-off valve 18 is fixedly connected to one side of each adapter pipe 17 through a bolt, a liquid discharging pipe 19 is fixedly connected to one side of each outer side on-off valve 18 through a bolt, and by using the outer side on-off valve 18 and the inner side on-off valve 16, the probability that umbilical cord blood stem cells in the cryopreservation device body 3 are polluted can be reduced, and the storage time of umbilical cord blood stem cells is prolonged.
The implementation scene is specifically as follows:
in the use of the present invention, cord blood stem cells in four parts of the freezing device body 3 are discharged from the inside of the extension tube 4 by the four inner on-off valves 16, so that cord blood stem cells in the freezing device body 3 can be taken out one by one without contaminating the cord blood stem cells in all the regions of the freezing device body 3.
Referring to the attached drawing 1 of the specification, 8, the cryopreservation device for low-temperature storage of umbilical cord blood stem cells of this embodiment, including cryopreservation device body 3, there is device closing cap 2 at the top of cryopreservation device body 3 through bolt fixedly connected with, the top of total control pole 5 is provided with extrusion sleeve 30, pole setting slot 28 has been seted up to the inside of extrusion sleeve 30 bottom, the top of total control pole 5 is pegged graft in the inside of pole setting slot 28, through the use of opposition pole slot 28, be convenient for connect extrusion sleeve 30 and total control pole 5, deflector 27 has been cup jointed to the inside of extrusion sleeve 30, the bottom fixedly connected with extrusion spring 29 of deflector 27, the bottom of extrusion spring 29 carries out fixed connection with the bottom inner wall of extrusion sleeve 30, the top fixedly connected with of deflector 27 seals dish 26, seal dish 26 swing joint is in the inside of device closing cap 2, through making extrusion spring 29 connect at deflector 27 and extrusion sleeve 30, be convenient for to the bottom extrusion total control seal dish 5 and prevent to be connected with device closing cap 2 and become flexible connecting seat 25 to the top of device, the top fixedly connected with rotation connecting seat 25, the control of threaded rod 23 is connected with the control device closing cap 23 through the top of hand wheel 2, the control threaded rod 23, the control device is convenient for the top of hand wheel closing cap 23 and hand wheel 2, the top of the control threaded rod is connected with the control device 1 of hand wheel 2 through the top of the hand wheel 2, the control device 1 of the hand wheel 2, the hand wheel 2.
The implementation scenario is specifically as follows:
in the using process of the invention, the control hand wheel 1 is rotated at the top of the device sealing cover 2 to adjust the threaded connection condition of the threaded rod 23 and the device sealing cover 2, the guide disc 27 connected with the sealing disc 26 is pushed towards the bottom, so that the extrusion sleeve 30 is utilized to push the master control vertical rod 5 to move towards the bottom, the outer wall of the bottom sealing block 15 is attached to the inner wall of the bottom sealing groove 22, the outer wall of the middle sealing block 13 is attached to the inner wall of the middle sealing groove 21, and the outer wall of the top sealing block 11 is attached to the inner wall of the top sealing groove 20, so that four inner cavities separated by the top partition plate 6, the middle partition plate 7 and the bottom partition plate 8 are prevented from being communicated with each other, meanwhile, the threaded rod 23 can be prevented from rotating under the effect of no external force by the extrusion spring 29 supported between the guide disc 27 and the extrusion sleeve 30, and the installation stability of the top sealing block 11, the middle sealing block 13 and the bottom sealing block 15 is ensured.
And finally: the present invention is not limited to the above preferred embodiments, but rather, any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A cryopreserved device for low-temperature storage of umbilical cord blood stem cells, comprises a cryopreserved device main body (3), and is characterized in that: the top of the freezing and storing device main body (3) is fixedly connected with a device sealing cover (2) through bolts, one side of the freezing and storing device main body (3) is fixedly connected with four external connecting pipes (4), one side of the external connecting pipes (4) is fixedly connected with an inner side on-off valve (16) through bolts, the bottom inner wall of the freezing and storing device main body (3) is fixedly connected with a bottom partition plate (8), a bottom sealing groove (22) is formed in the center of the bottom partition plate (8), a bottom sealing block (15) is inserted in the bottom sealing groove (22), the outer wall of the bottom sealing block (15) is attached to the inner wall of the bottom sealing groove (22), the middle inner wall of the freezing and storing device main body (3) is fixedly connected with a middle partition plate (7), a middle sealing groove (21) is formed in the center of the middle partition plate (7), a middle sealing block (13) is inserted in the middle sealing groove (21), the outer wall of the middle sealing block (13) is attached to the inner wall of the middle sealing groove (21), the top partition plate (6) is fixedly connected with a top partition plate (6), and the top sealing block (20) is inserted in the top sealing groove (11), the inside fixedly connected with of top seal block (11), middle part seal block (13) and bottom seal block (15) is the same always controls pole setting (5).
2. The cryopreservation device for umbilical cord blood stem cell storage according to claim 1, wherein: one side of the inner side on-off valve (16) is fixedly connected with an adapter tube (17) through a bolt, one side of the adapter tube (17) is fixedly connected with an outer side on-off valve (18) through a bolt, and one side of the outer side on-off valve (18) is fixedly connected with a liquid discharging pipe (19) through a bolt.
3. The cryopreservation device for cord blood stem cells storage at low temperature according to claim 1, characterized in that: the bottom inner wall fixedly connected with deflector (9) of device main part (3) is deposited to freezing, the cross-section of deflector (9) is triangle-shaped.
4. The cryopreservation device for umbilical cord blood stem cell storage according to claim 1, wherein: the diameter of the top circle of the bottom sealing block (15) is smaller than that of the bottom circle of the middle sealing block (13), and the diameter of the top circle of the middle sealing block (13) is smaller than that of the bottom circle of the top sealing block (11).
5. The cryopreservation device for cord blood stem cells storage at low temperature according to claim 1, characterized in that: the top fixedly connected with top direction round platform (10) of top seal piece (11), the top fixedly connected with middle part direction round platform (12) of middle part seal piece (13), the top fixedly connected with bottom direction round platform (14) of bottom seal piece (15).
6. The cryopreservation device for cord blood stem cells storage at low temperature according to claim 1, characterized in that: the top of total accuse pole setting (5) is provided with extrusion sleeve (30), deflector (27) have been cup jointed to the inside of extrusion sleeve (30), the bottom fixedly connected with extrusion spring (29) of deflector (27), the bottom of extrusion spring (29) carries out fixed connection with the bottom inner wall of extrusion sleeve (30), the top fixedly connected with sealed dish (26) of deflector (27), sealed dish (26) swing joint is in the inside of device closing cap (2).
7. The cryopreservation device for cord blood stem cell storage according to claim 6, wherein: the top fixedly connected with of sealed dish (26) rotates connecting seat (25), the inside swing joint that rotates connecting seat (25) has spacing dish (24), the top fixedly connected with threaded rod (23) of spacing dish (24), threaded rod (23) threaded connection is in the inside of device closing cap (2).
8. The cryopreservation device for umbilical cord blood stem cell storage according to claim 7, wherein: the top fixedly connected with control hand wheel (1) of threaded rod (23), control hand wheel (1) is located the top of device closing cap (2).
9. The cryopreservation device for cord blood stem cell storage according to claim 6, wherein: the inside of extrusion sleeve (30) bottom has seted up pole setting slot (28), the top of always controlling pole setting (5) is pegged graft in the inside of pole setting slot (28).
10. The cryopreservation device for cord blood stem cells storage at low temperature according to claim 1, characterized in that: the cross sections of the outer side on-off valve (18), the bottom sealing groove (22), the middle sealing groove (21), the middle sealing block (13), the top sealing block (11) and the top sealing groove (20) are all trapezoidal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210586694.6A CN115176794A (en) | 2022-05-30 | 2022-05-30 | Freezing storage device for low-temperature storage of umbilical cord blood stem cells |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210586694.6A CN115176794A (en) | 2022-05-30 | 2022-05-30 | Freezing storage device for low-temperature storage of umbilical cord blood stem cells |
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| CN115176794A true CN115176794A (en) | 2022-10-14 |
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| CN202210586694.6A Pending CN115176794A (en) | 2022-05-30 | 2022-05-30 | Freezing storage device for low-temperature storage of umbilical cord blood stem cells |
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| CN215819755U (en) * | 2021-09-22 | 2022-02-15 | 上海五心医院管理有限公司 | Device is deposited in freezing of umbilical cord blood sample that contains cord blood stem cell |
| CN216292770U (en) * | 2021-09-08 | 2022-04-15 | 上海五心医院管理有限公司 | Cooling device used in stem cell cryopreservation process |
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Application publication date: 20221014 |