CN216292770U - Cooling device used in stem cell cryopreservation process - Google Patents
Cooling device used in stem cell cryopreservation process Download PDFInfo
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- CN216292770U CN216292770U CN202122166102.9U CN202122166102U CN216292770U CN 216292770 U CN216292770 U CN 216292770U CN 202122166102 U CN202122166102 U CN 202122166102U CN 216292770 U CN216292770 U CN 216292770U
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- stem cells
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 44
- 238000001816 cooling Methods 0.000 title claims abstract description 20
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 16
- 230000008569 process Effects 0.000 title claims abstract description 14
- 230000007246 mechanism Effects 0.000 claims abstract description 14
- 230000006835 compression Effects 0.000 claims abstract description 12
- 238000007906 compression Methods 0.000 claims abstract description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 60
- 239000007788 liquid Substances 0.000 claims description 34
- 229910052757 nitrogen Inorganic materials 0.000 claims description 30
- 238000007710 freezing Methods 0.000 claims description 29
- 230000008014 freezing Effects 0.000 claims description 29
- 238000004378 air conditioning Methods 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 6
- 230000007306 turnover Effects 0.000 claims description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 5
- 235000017491 Bambusa tulda Nutrition 0.000 claims 5
- 241001330002 Bambuseae Species 0.000 claims 5
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 5
- 239000011425 bamboo Substances 0.000 claims 5
- 238000000151 deposition Methods 0.000 claims 1
- 238000005192 partition Methods 0.000 abstract description 10
- 230000000712 assembly Effects 0.000 abstract description 4
- 238000000429 assembly Methods 0.000 abstract description 4
- 239000011550 stock solution Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 9
- 230000009471 action Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011490 mineral wool Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
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Abstract
The utility model discloses a cooling device used in a stem cell cryopreservation process, which comprises a box body and an oscillating mechanism, wherein a first partition plate is arranged in the box body, the oscillating mechanism comprises a motor and a rotating plate, a rotating rod is installed at the center of the bottom of the rotating plate, a supporting plate is arranged above the rotating plate, oscillating assemblies are arranged at four vertex angles of the top of the rotating plate one by one, each oscillating assembly comprises a limiting cylinder, electromagnets are symmetrically installed on an inner top plate of the limiting cylinder, permanent magnets are connected in the limiting cylinder in a sliding mode, a compression spring is arranged below the permanent magnets, a moving rod is installed at the center of the top of the permanent magnets, and a connecting plate is arranged on one side of the moving rod. According to the utility model, stem cells and the frozen stock solution can be uniformly mixed through the matching of the motor, the rotating rod, the rotating plate, the supporting plate, the electromagnet, the compression spring, the permanent magnet and the moving rod, and the device is simple to operate, time-saving and labor-saving.
Description
Technical Field
The utility model relates to the field of stem cell cryopreservation equipment, in particular to a cooling device used in a stem cell cryopreservation process.
Background
Conventionally, stem cells are collected and processed, and then the stem cells and the frozen solution are mixed together by adding the frozen solution, and then stored at an ultralow temperature, for example, minus 196 ℃ or even lower, and the stem cells are mixed while being cooled slowly from room temperature to the ultralow temperature, so that the stem cells and the frozen solution are uniformly mixed and cooled.
Although the existing cooling device can complete the precooling of stem cells, an operator needs to shake the freezing tube manually to mix the stem cells with the freezing liquid, so that the mixing is not uniform enough, and the operation is troublesome and the time is wasted.
Therefore, it is necessary to provide a cooling device for use in the process of freezing stem cells to solve the above problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a cooling device used in a stem cell freezing process, which solves the problems that although the conventional cooling device can complete the cooling and freezing of stem cells, an operator needs to shake a freezing tube manually to mix the stem cells with freezing liquid, so that the stem cells and the freezing liquid are not mixed uniformly, the operation is troublesome and the time is wasted.
In order to achieve the above purpose, the utility model provides the following technical scheme: a cooling device used in a stem cell cryopreservation process comprises a box body and an oscillating mechanism, wherein a first partition plate is arranged in the box body, the oscillating mechanism comprises a motor and a rotating plate, a rotating rod is installed at the center of the bottom of the rotating plate, the bottom end of the rotating rod penetrates through the first partition plate, a supporting plate is arranged above the rotating plate, four vertex angles at the top of the rotating plate are provided with oscillating assemblies one by one, each oscillating assembly comprises a limiting cylinder, the limiting cylinders are fixed at the top of the rotating plate, electromagnets are symmetrically installed on an inner top plate of each limiting cylinder, permanent magnets are slidably connected in the limiting cylinders, compression springs are arranged below the permanent magnets, the compression springs are fixed at the top of the rotating plate, a moving rod is installed at the center of the top of each permanent magnet, and the top ends of the moving rods penetrate through the limiting cylinders and extend to the supporting plate, one side of carriage release lever is equipped with the connecting plate, connecting plate and backup pad fixed connection, the connecting plate rotates with the carriage release lever to be connected, just the motor is fixed in the bottom half, the output shaft of motor runs through the box and extends to dwang department, the output shaft and the box of motor rotate to be connected, the output shaft and the dwang fixed connection of motor.
Preferably, the top of backup pad is equipped with freezes deposits the box, it freezes deposits the pipe to be equipped with in the box to freeze to deposit, it is equipped with a plurality of to freeze to deposit the pipe, just it all is equipped with clamping mechanism, every all to freeze to deposit the box all around clamping mechanism includes the riser, the riser is fixed in the backup pad top, it is provided with the screw sleeve to alternate on the riser, the screw sleeve rotates with the riser to be connected, screw sleeve inner gearing is connected with the threaded rod, clamp plate and limiting plate are installed respectively to the both ends of threaded rod.
Preferably, the first partition plate is fixedly connected with the inner wall of the box body, the top of the first partition plate is divided into a vacuum cavity and a freezing cavity through a second partition plate, and the second partition plate is fixedly connected with the first partition plate and the box body.
Preferably, the bottom of first baffle is installed the heated board, be equipped with the blast pipe in the heated board, be equipped with the shunt tubes on the blast pipe, the shunt tubes is equipped with a plurality of, blast pipe and first baffle are run through respectively at the both ends of shunt tubes, just the business turn over wind gap of blast pipe all runs through the box, install three way connection on the air intake of blast pipe.
Preferably, the place ahead of box is equipped with the refrigerator, one side of refrigerator is equipped with the liquid nitrogen container, just the liquid nitrogen outlet of liquid nitrogen container and the cold air export of refrigerator all link to each other through pipeline and three way connection, all install the control valve on the pipeline between three way connection and liquid nitrogen container liquid nitrogen export and the cold air export of refrigerator.
Preferably, the movable rod is connected with the limiting cylinder in a sliding mode, and a sealing ring is installed at the joint of the movable rod and the limiting cylinder.
Preferably, a sealing cover plate is installed on an opening at the top of the box body, and supporting legs are installed at four top corners of the bottom of the box body one by one.
In the technical scheme, the utility model provides the following technical effects and advantages:
1. the stem cells and the frozen solution can be uniformly mixed by matching the motor, the rotating rod, the rotating plate, the supporting plate, the electromagnet, the compression spring, the permanent magnet and the moving rod, and the method is simple to operate, time-saving and labor-saving;
2. the threaded rod drives the clamping plate to move by rotating the threaded sleeve, the freezing storage box can be clamped by the movable clamping plate, the freezing storage box is fixed, the freezing storage box is convenient to disassemble, and operators can conveniently take and place the freezing storage box;
3. the refrigerator through setting provides air conditioning, can carry out the precooling to the stem cell, provide the liquid nitrogen that the cryopreserved stem cell needs through the liquid nitrogen container, can carry air conditioning or liquid nitrogen to the blast pipe in through opening different control valves, can be with the comparatively even transport of air conditioning or liquid nitrogen in the blast pipe to the cryopreserved intracavity through the shunt tubes that set up, can improve the effect that the stem cell cryopreserved, and adopted two kinds of cooling methods, can not only satisfy different temperature conditions, can also reduce to get the number of times of putting the cryopreserved pipe, and it is more convenient to use.
Drawings
In order to more clearly illustrate the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to the drawings.
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a sectional view of the structure of the case of the present invention;
FIG. 3 is an enlarged view taken at A of FIG. 2 in accordance with the present invention;
FIG. 4 is a cross-sectional view of an oscillating assembly according to the present invention.
Description of reference numerals:
1. a box body; 2. a liquid nitrogen tank; 3. a freezer; 4. a three-way joint; 5. a first separator; 6. a second separator; 7. a vacuum chamber; 8. freezing and storing the box; 9. a thermal insulation board; 10. an air supply pipe; 11. a motor; 12. rotating the rod; 13. a rotating plate; 14. a support plate; 15. a limiting cylinder; 16. a permanent magnet; 17. a compression spring; 18. a travel bar; 19. an electromagnet; 20. a seal ring; 21. a connecting plate; 22. freezing and storing the tube; 23. a vertical plate; 24. a clamping plate; 25. a threaded sleeve; 26. a threaded rod; 27. a limiting plate; 28. and (4) controlling the valve.
Detailed Description
In order to make the technical solutions of the present invention better understood, those skilled in the art will now describe the present invention in further detail with reference to the accompanying drawings.
The utility model provides a cooling device used in a stem cell cryopreservation process, as shown in fig. 1-4, comprising a box body 1 and an oscillating mechanism, wherein a first partition plate 5 is arranged in the box body 1, the oscillating mechanism comprises a motor 11 and a rotating plate 13, a rotating rod 12 is arranged at the center of the bottom of the rotating plate 13, the bottom end of the rotating rod 12 penetrates through the first partition plate 5, a support plate 14 is arranged above the rotating plate 13, oscillating assemblies are arranged at four top corners of the top of the rotating plate 13 one by one, each oscillating assembly comprises a limiting cylinder 15, the limiting cylinders 15 can limit the positions of the support plates 14, the limiting cylinders 15 are fixed at the top of the rotating plate 13, electromagnets 19 are symmetrically arranged on the inner top plate of the limiting cylinders 15, how to control the on-off of the electromagnets 19 is the prior art, and can be controlled by a controller, such as a PLC controller, which is not specifically described herein, in the utility model, at most two permanent magnets 16 in the oscillating assemblies are electrified at one time, the permanent magnets 16 are connected in the limiting cylinder 15 in a sliding manner, a compression spring 17 is arranged below the permanent magnets 16, the compression spring 17 is fixed on the top of the rotating plate 13, a moving rod 18 is installed at the center of the top of each permanent magnet 16, the top end of each moving rod 18 penetrates through the limiting cylinder 15 and extends to the position of the supporting plate 14, a connecting plate 21 is arranged on one side of each moving rod 18, the connecting plate 21 is fixedly connected with the supporting plate 14, the connecting plate 21 is rotatably connected with the moving rod 18, the motor 11 is fixed at the bottom of the box body 1, the output shaft of the motor 11 penetrates through the box body 1 and extends to the position of the rotating rod 12, the output shaft of the motor 11 is rotatably connected with the box body 1, and the output shaft of the motor 11 is fixedly connected with the rotating rod 12.
Further, in the above technical solution, the top of the supporting plate 14 is provided with a freezing storage box 8, the freezing storage box 8 is internally provided with a freezing storage tube 22, the freezing storage tube 22 is provided with a plurality of freezing storage tubes 22, the number of the freezing storage tubes 22 is set according to actual conditions, at most, the hole grooves in the freezing storage box 8 are filled, the freezing storage tubes 22 are placed in the freezing storage box 8, and the frozen dry cells can be conveniently transferred subsequently, the periphery of the freezing storage box 8 is provided with clamping mechanisms, each clamping mechanism comprises a vertical plate 23, the vertical plate 23 is fixed at the top of the supporting plate 14, the vertical plate 23 is internally provided with a threaded sleeve 25 in a penetrating manner, the threaded sleeve 25 is rotatably connected with the vertical plate 23, the threaded sleeve 25 is connected with a threaded rod 26, two ends of the threaded rod 26 are respectively provided with a clamping plate 24 and a limiting plate 27, the limiting plate 27 is arranged to limit the position of the threaded rod 26, and the threaded rod 26 is driven by rotating the threaded sleeve 25 to move the clamping plate 24, utilize the clamp plate 24 that removes to press from both sides tight cryopreserving box 8, realize cryopreserving box 8's fixed, and dismantle the convenience, the operating personnel of being convenient for gets and puts cryopreserving box 8.
Further, in above-mentioned technical scheme, first baffle 5 and 1 inner wall fixed connection of box, 5 tops of first baffle are divided into vacuum chamber 7 and cryopreserved chamber through second baffle 6, second baffle 6 and first baffle 5 and 1 fixed connection of box, during the preparation, to vacuum chamber 7 evacuation, it is effectual to keep warm, can reduce ambient temperature and cause harmful effects to stem cell cryopreserving.
Further, in above-mentioned technical scheme, heated board 9 is installed to the bottom of first baffle 5, and heated board 9 can adopt insulation material preparation such as polyurethane, mineral wool, has the heat preservation effect, can further reduce external environment temperature and freeze and deposit and cause the influence to stem cell, be equipped with blast pipe 10 in the heated board 9, be equipped with the shunt tubes on the blast pipe 10, the shunt tubes is equipped with a plurality of, blast pipe 10 and first baffle 5 are run through respectively at the both ends of shunt tubes, just the business turn over wind gap of blast pipe 10 all runs through box 1, install three way connection 4 on the air intake of blast pipe 10, can be with comparatively even transport to the cryopreserving intracavity of air conditioning or liquid nitrogen in the blast pipe 10 through the shunt tubes that sets up, can improve the effect that the stem cell freezes and deposits.
Further, in above-mentioned technical scheme, the place ahead of box 1 is equipped with refrigerator 3, provides air conditioning through the refrigerator 3 that sets up, can carry out the precooling to the stem cell, one side of refrigerator 3 is equipped with liquid nitrogen container 2, provides the liquid nitrogen that the cryopreserved stem cell needs through liquid nitrogen container 2, and the activity of keeping the stem cell that the liquid nitrogen can be fine, liquid nitrogen outlet of liquid nitrogen container 2 and the air conditioning export of refrigerator 3 all link to each other with three way connection 4 through the pipeline, all install control valve 28 on the pipeline between three way connection 4 and the 2 liquid nitrogen outlets of liquid nitrogen container and the 3 air conditioning exports of refrigerator, can carry air conditioning or liquid nitrogen to blast pipe 10 in through opening different control valve 28, it is more convenient to use.
Further, in the above technical scheme, the moving rod 18 is connected with the limiting cylinder 15 in a sliding manner, the sealing ring 20 is installed at the joint of the moving rod 18 and the limiting cylinder 15, and the arranged sealing ring 20 can be made of rubber or silica gel materials, has a sealing effect, and can effectively prevent dust from entering the limiting cylinder 15.
Further, in the above technical scheme, install sealed apron on the open-top of box 1, sealed apron adopts the hinge to install on box 1, still can set up the handle on the sealed apron, makes things convenient for operating personnel to open and shut sealed apron, the supporting legs is installed one by one to four apex angle departments of box 1 bottom, and the supporting legs of setting have the effect of supporting box 1.
This practical theory of operation:
referring to the attached drawings 1, 2 and 4 of the specification, when pre-cooling stem cells, a rotating rod 12 is driven by positive and negative rotation of a motor 11 to drive a rotating plate 13 to rotate, a supporting plate 14 is driven by the rotating plate 13 to rotate, so that a freezing tube 22 rotates, stem cells and freezing liquid are mixed under the action of centrifugal force, meanwhile, an electromagnet 19 in one (or two) oscillating assembly is powered on and then powered off, the permanent magnet 16 drives a moving rod 18 to move upwards by the magnetic force generated by the powered electromagnet 19 and the elastic force of a compression spring 17, one end of the supporting plate 14 is lifted by the moving rod 18 moving upwards, after the electromagnet 19 is powered off, the magnetic force disappears, the supporting plate 14 drives the moving rod 18 to return to the original position under the action of the gravity, meanwhile, the permanent magnet 16 can press the compression spring 17, and after the operations are repeated for multiple times, the supporting plate 14 drives the freezing tube 22 to swing upwards and downwards, thereby enabling stem cells and the cryopreservation solution to be uniformly mixed, and having simple operation, time saving and labor saving;
referring to the attached drawings 1, 2 and 3 of the specification, the cryopreservation tube 22 is placed in the cryopreservation box 8, so that the cryopreserved stem cells can be conveniently transferred subsequently, the threaded rod 26 drives the clamping plate 24 to move by rotating the threaded sleeve 25, the cryopreservation box 8 can be clamped by the movable clamping plate 24, the fixation of the cryopreservation box 8 is realized, the cryopreservation box is convenient to disassemble, and an operator can conveniently take and place the cryopreservation box 8;
refer to description attached drawing 1 and 2, refrigerator 3 through the setting provides air conditioning, can carry out the precooling to the stem cell, provide the liquid nitrogen that the cryopreserved stem cell needs through liquid nitrogen container 2, the activity of keeping the stem cell that the liquid nitrogen can be fine, can carry air conditioning or liquid nitrogen to blast pipe 10 in through opening different control valve 28, can be with the comparatively even transport of air conditioning or liquid nitrogen in blast pipe 10 to the cryopreserved intracavity through the shunt tubes that set up, can improve the effect that the stem cell cryopreserved, two kinds of cooling methods have been adopted simultaneously, can not only satisfy different temperature conditions, can also reduce the number of times of getting and putting cryopreserved pipe 22, and it is more convenient to use.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the utility model. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the utility model.
Claims (7)
1. The utility model provides a heat sink that stem cell cryopreserved in-process used, includes box (1) and vibrates the mechanism, its characterized in that: be equipped with first baffle (5) in box (1), it includes motor (11) and rotor plate (13) to vibrate the mechanism, the bottom center department of rotor plate (13) installs dwang (12), the bottom of dwang (12) runs through first baffle (5), just the top of rotor plate (13) is equipped with backup pad (14), four apex angle departments at rotor plate (13) top are equipped with one by one and vibrate the subassembly, every it all includes spacing section of thick bamboo (15) to vibrate the subassembly, spacing section of thick bamboo (15) are fixed in rotor plate (13) top, electromagnet (19) are installed to the symmetry on the interior roof of spacing section of thick bamboo (15), sliding connection has permanent magnet (16) in spacing section of thick bamboo (15), the below of permanent magnet (16) is equipped with compression spring (17), compression spring (17) are fixed in rotor plate (13) top, just permanent magnet (16) top center department installs carriage release lever (18), the top of carriage release lever (18) runs through spacing section of thick bamboo (15) and extends to backup pad (14) department, one side of carriage release lever (18) is equipped with connecting plate (21), connecting plate (21) and backup pad (14) fixed connection, connecting plate (21) rotate with carriage release lever (18) and are connected, just motor (11) are fixed in box (1) bottom, the output shaft of motor (11) runs through box (1) and extends to dwang (12) department, the output shaft and the box (1) rotation of motor (11) are connected, the output shaft and dwang (12) fixed connection of motor (11).
2. The cooling device for use in the cryopreservation process of stem cells according to claim 1, wherein: the top of backup pad (14) is equipped with freezes deposits box (8), it freezes deposits pipe (22) to be equipped with in freezing deposits box (8), it is equipped with a plurality of to freeze deposits pipe (22), just freeze and all be equipped with clamping mechanism, every around depositing box (8) clamping mechanism all includes riser (23), riser (23) are fixed in backup pad (14) top, it is provided with threaded sleeve (25) to alternate on riser (23), threaded sleeve (25) rotate with riser (23) and are connected, threaded sleeve (25) inner gearing is connected with threaded rod (26), grip block (24) and limiting plate (27) are installed respectively to the both ends of threaded rod (26).
3. The cooling device for use in the cryopreservation process of stem cells according to claim 1, wherein: first baffle (5) and box (1) inner wall fixed connection, first baffle (5) top has vacuum chamber (7) and freezes deposits the chamber through second baffle (6), second baffle (6) and first baffle (5) and box (1) fixed connection.
4. The cooling device for use in the cryopreservation process of stem cells according to claim 3, wherein: heated board (9) are installed to the bottom of first baffle (5), be equipped with blast pipe (10) in heated board (9), be equipped with the shunt tubes on blast pipe (10), the shunt tubes is equipped with a plurality of, blast pipe (10) and first baffle (5) are run through respectively to the both ends of shunt tubes, just box (1) is all run through at the business turn over wind gap of blast pipe (10), install three way connection (4) on the air intake of blast pipe (10).
5. The cooling device for use in the cryopreservation process of stem cells according to claim 4, wherein: the place ahead of box (1) is equipped with refrigerator (3), one side of refrigerator (3) is equipped with liquid nitrogen container (2), just the liquid nitrogen export of liquid nitrogen container (2) and the air conditioning export of refrigerator (3) all link to each other with three way connection (4) through the pipeline, all install control valve (28) on the pipeline between three way connection (4) and liquid nitrogen container (2) liquid nitrogen export and refrigerator (3) air conditioning export.
6. The cooling device for use in the cryopreservation process of stem cells according to claim 1, wherein: the movable rod (18) is connected with the limiting cylinder (15) in a sliding mode, and a sealing ring (20) is installed at the connecting position of the movable rod (18) and the limiting cylinder (15).
7. The cooling device for use in the cryopreservation process of stem cells according to claim 1, wherein: the top opening of box (1) is last to install sealed apron, four apex angle departments of box (1) bottom install the supporting legs one by one.
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CN202122166102.9U CN216292770U (en) | 2021-09-08 | 2021-09-08 | Cooling device used in stem cell cryopreservation process |
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CN202122166102.9U CN216292770U (en) | 2021-09-08 | 2021-09-08 | Cooling device used in stem cell cryopreservation process |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115176794A (en) * | 2022-05-30 | 2022-10-14 | 五心生物科技(福建)有限公司 | Freezing storage device for low-temperature storage of umbilical cord blood stem cells |
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2021
- 2021-09-08 CN CN202122166102.9U patent/CN216292770U/en active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115176794A (en) * | 2022-05-30 | 2022-10-14 | 五心生物科技(福建)有限公司 | Freezing storage device for low-temperature storage of umbilical cord blood stem cells |
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Address after: Room 526, 5th Floor, Building 1, No. 6 Kangye Road, Zhujiajiao Town, Qingpu District, Shanghai, 201713 Patentee after: Shanghai Wuxin Medical Technology Group Co.,Ltd. Country or region after: China Address before: 201109 room 218, No. 2, No. 3399, Yuanjiang Road, Minhang District, Shanghai Patentee before: Shanghai Wuxin Hospital Management Co.,Ltd. Country or region before: China |
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