CN108216931B

CN108216931B - Low-temperature preservation box for umbilical cord blood stem cells

Info

Publication number: CN108216931B
Application number: CN201810013259.8A
Authority: CN
Inventors: 曾珣; 谷昌德
Original assignee: West China Second University Hospital of Sichuan University
Current assignee: West China Second University Hospital of Sichuan University
Priority date: 2018-01-06
Filing date: 2018-01-06
Publication date: 2020-05-15
Anticipated expiration: 2038-01-06
Also published as: CN108216931A

Abstract

The invention relates to a low-temperature umbilical cord blood stem cell storage box, relates to the field of medical instruments, and mainly aims to solve the technical problem that the freezing effect of stem cells is influenced by long taking-out time of a lower-layer collection bag in the conventional freezing storage box. The main technical scheme adopted is as follows: the cord blood stem cell low-temperature preservation box comprises a box body, a collection bag containing mechanism and a position adjusting mechanism. The box body is provided with a box cover and a taking and placing opening positioned on the side surface, and the box cover can be opened and closed relative to the taking and placing opening; the collecting bag accommodating mechanism comprises a supporting plate and a pressing plate which are arranged in parallel, and the pressing plate is positioned above the supporting plate; the pressing plate can move relative to the supporting plate so as to form an accommodating gap for placing the collecting bag when the pressing plate moves to the first position, and a gap opening of the accommodating gap is opposite to the taking and placing opening; and applying pressure to the collection bag when moving to the second position; the position adjusting mechanism is used for being matched with the box cover so as to enable the pressing plate to be located at the first position when the box cover is opened; and the pressure plate is located in the second position when the cover is closed.

Description

Low-temperature preservation box for umbilical cord blood stem cells

Technical Field

The invention relates to the technical field of medical appliances, in particular to a cord blood stem cell low-temperature storage box.

Background

Human cord blood stem cells are usually stored in a cryogenic refrigerator/liquid nitrogen tank at-85 ℃ or-196 ℃ depending on the desired age of storage. For cord blood stem cells with a short period of time after collection (< 6 months), cryopreservation at-85 ℃ is usually performed by using a deep low temperature refrigerator. In a specific operation, the cord blood stem cells are usually collected in a plastic collection bag, then are contained by using a container with a specific structure, such as a freezing storage box, and the freezing storage box is put into a low-temperature refrigerator to cool the cord blood stem cells of a human body.

At present, a freezing storage box is generally provided with an opening at the top, the collection bags filled with cord blood stem cells are sequentially placed in the storage box from top to bottom, a partition plate is arranged between two adjacent collection bags, and when a box cover is closed, the box cover applies pressure to the collection bags inside to flatten the collection bags. The above preservation method has the following disadvantages and clinical risks: if need take out the collection bag of lower floor, need take out the collection bag and the baffle on upper strata earlier, so operation is more loaded down with trivial details, and this operation also makes the time overlength of opening the freezing preservation case moreover, causes to preserve the incasement temperature and can not be invariable, influences the freezing effect of whole preservation incasement stem cell.

Disclosure of Invention

In view of the above, the invention provides a cord blood stem cell low-temperature storage box, and mainly aims to solve the technical problems that the storage box is too long in opening time and the freezing effect of stem cells is influenced due to the fact that the taking-out operation of a lower-layer collection bag in the existing freezing storage box is complicated.

In order to achieve the purpose, the invention mainly provides the following technical scheme:

an embodiment of the present invention provides a cryopreservation box for umbilical cord blood stem cells, which is used for cryopreservation of umbilical cord blood stem cells placed in a collection bag, and comprises:

the box body is provided with a box cover and a taking and placing opening positioned on the side face, and the box cover can be opened and closed relative to the taking and placing opening;

the collecting bag accommodating mechanism comprises a supporting plate and a pressing plate which are arranged in parallel, the supporting plate and the pressing plate are both positioned in the box body, and the pressing plate is positioned above the supporting plate; the pressing plate can move relative to the supporting plate so as to form an accommodating gap for placing a collection bag when moving to a first position, and a gap opening of the accommodating gap is opposite to the taking and placing opening; and applying pressure to the collection bag when moving to the second position; and

the position adjusting mechanism is used for being matched with the box cover so as to enable the pressing plate to be located at the first position when the box cover is opened; and positioning the pressure plate in the second position when the lid is closed;

the position adjusting mechanism comprises a pressing block and a movable block;

a limiting groove is formed in the upper side of the pressing plate, and the pressing block is inserted into the limiting groove from the upper side; one end of the pressing block, which is far away from the pressing plate, is provided with a first inclined surface;

the movable block is arranged in the box body and is positioned above the pressing plate; the movable block is provided with a first end and a second end which are opposite to each other, and the first end is close to the pressing block relative to the second end; the first end is provided with a second inclined surface matched with the first inclined surface, and the movable block is used for applying force to the first inclined surface through the second inclined surface when moving to a first movable position so as to enable the pressing block to push the pressing plate to move to a second position; the box cover is used for applying force to the second end of the movable block when being closed so as to push the movable block to move to the first movable position;

the number of the collecting bag accommodating mechanisms is more than two, and the collecting bag accommodating mechanisms are sequentially arranged from top to bottom; the number of the position adjusting mechanisms is equal to that of the collecting bag containing mechanisms, and the position adjusting mechanisms are arranged in a one-to-one correspondence manner.

By means of the technical scheme, the low-temperature preservation box for the cord blood stem cells has at least the following beneficial effects:

in the technical scheme provided by the invention, the taking and placing opening of the box body is positioned on the side surface, and the gap opening of the accommodating gap between the pressing plate and the supporting plate for placing the collecting bag is opposite to the taking and placing opening, so that the corresponding collecting bag can be taken and placed from the taking and placing opening on the side surface in a loose manner without operating other collecting bags in the box body, such as taking and placing, and the like, and further, compared with the prior art, the time for taking out the lower-layer collecting bag can be saved, and the freezing effect of other collecting bags in the storage box is prevented from being influenced by long-time operation.

The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.

Drawings

Fig. 1 is a schematic structural view of a cord blood stem cell cryopreservation box according to an embodiment of the invention, with the box cover opened;

fig. 2 is a schematic structural diagram of a cover of a cord blood stem cell cryopreservation box according to an embodiment of the invention when the cover is closed;

fig. 3 is a schematic structural diagram illustrating an assembly of a fixed plate and a movable block of a cryopreservation box for cord blood stem cells according to an embodiment of the invention;

FIG. 4 is a schematic structural diagram of a movable block of a cryopreservation box for cord blood stem cells according to an embodiment of the invention;

FIG. 5 is a schematic diagram of a block and plate assembly of a cryopreservation box for cord blood stem cells according to an embodiment of the invention;

fig. 6 is a schematic structural diagram of a pressing plate of an umbilical cord blood stem cell cryopreservation box according to an embodiment of the invention.

Reference numerals: 1. a box body; 10. collecting bags; 11. a box cover; 100. a low-temperature preservation box for umbilical cord blood stem cells; 101. a taking and placing port; 111. a first bump; 2. a collection bag housing mechanism; 21. A first collection bag containment mechanism; 22. a second collection bag holding mechanism; 3. a support plate; 4. pressing a plate; 41. a limiting groove; 401. a first limit bump; 402. a second limit bump; 5. a receiving gap; 6. a position adjustment mechanism; 61. a first position adjustment mechanism; 62. a second position adjustment mechanism; 7. briquetting; 71. a first inclined plane; 8. a movable block; 81. a second inclined plane; 82. A second bump; 821. a second support column; 9. a slide rail; 91. a support block; 911. a first support column; 12. a second elastic member; 13. a limiting block; 14. a guide post; 15. and (7) fixing the plate.

Detailed Description

To further explain the technical means and effects of the present invention adopted to achieve the predetermined object, the following detailed description of the embodiments, structures, features and effects according to the present invention will be made with reference to the accompanying drawings and preferred embodiments. In the following description, different "one embodiment" or "an embodiment" refers to not necessarily the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

As shown in fig. 1 and 2, an embodiment of the present invention provides a cryopreservation chamber 100 for umbilical cord blood stem cells, which is used for cryopreservation of umbilical cord blood stem cells placed in a collection bag 10. The cord blood stem cell cryopreservation box 100 includes a box body 1, a collection bag housing mechanism 2, and a position adjusting mechanism 6. The case 1 has a case cover 11 and a pick-and-place port 101 on a side surface. The box cover 11 can be opened and closed relative to the taking and placing opening 101.

As shown in fig. 1 and 2, the collection bag housing mechanism 2 includes a support plate 3 and a pressing plate 4 arranged in parallel. The supporting plate 3 and the pressing plate 4 are both positioned in the box body 1. The pressing plate 4 is located above the supporting plate 3. The pressure plate 4 is movable to a first position and a second position relative to the support plate 3. As shown in fig. 1, when the pressing plate 4 moves to the first position relative to the supporting plate 3, a receiving gap 5 for placing the collection bag 10 is formed between the pressing plate 4 and the supporting plate 3, and the gap opening of the receiving gap 5 is opposite to the access opening 101, so that the user can conveniently access the collection bag 10 filled with stem cells from the receiving gap 5 through the access opening 101. As shown in fig. 2, when the pressure plate 4 is moved to the second position, the pressure plate 4 applies pressure to the collection bag 10, and the collection bag 10 is flattened and held flat under the application of pressure, thereby helping to maintain a uniform temperature drop throughout the interior of the collection bag 10 when the collection bag 10 is placed in a refrigerator.

The position adjusting mechanism 6 is used for cooperating with the box cover 11 to enable the pressing plate 4 to be located at a first position (as shown in fig. 1) when the box cover 11 is opened, so that a user can conveniently take and place the collecting bag 10; and with the cover 11 closed, the pressure plate 4 in the second position (as shown in fig. 2), the bag 10 is flattened under pressure.

In the technical scheme provided above, because the access opening 101 of box 1 is located the side, the clearance mouth that is used for placing the accommodation gap 5 of gathering the bag 10 between clamp plate 4 and the backup pad 3 also is relative with access opening 101, thereby can easily get corresponding gathering bag 10 from access opening 101 of side, and need not to operate other gathering bags 10 in box 1 such as get and put, and then can save the time of taking out lower floor's gathering bag 10 for prior art, avoid long-time operation to influence the freezing effect of other gathering bags 10 in the storage box.

In one specific example of use, as shown in fig. 1 and 2, both the aforementioned support plate 3 and the pressure plate 4 may be horizontally disposed, such that the collection bag 10 is also horizontally disposed within the receiving space 5 between the support plate 3 and the pressure plate 4. When the collection bag 10 is pressurized, it helps to provide uniform pressure throughout the interior of the collection bag 10, thereby providing more uniform cooling throughout the interior of the collection bag 10.

Further, as shown in fig. 1 and 2, the number of the collecting bag accommodating mechanisms 2 may be two or more, and the collecting bag accommodating mechanisms are arranged in sequence from top to bottom. The number of the position adjusting mechanisms 6 is equal to that of the collecting bag accommodating mechanisms 2, and the position adjusting mechanisms are arranged in a one-to-one correspondence manner. During operation, only one collection bag 10 filled with stem cells is placed in each collection bag containing mechanism 2, so that the collection bags 10 can be separated through the collection bag containing mechanisms 2, and adhesion between adjacent collection bags 10 in a low-temperature freezing state is avoided.

Further, as shown in fig. 1 and 2, the aforementioned position adjustment mechanism 6 may include a pressing block 7 and a movable block 8. The pressing block 7 is disposed on the upper side of the pressing plate 4. The end of the pressure piece 7 facing away from the pressure plate 4 has a first bevel 71 (shown in fig. 5). The movable block 8 is arranged in the box body 1 and is positioned above the pressing plate 4. The movable block 8 has a first end and a second end opposite to each other. The first end is close to the pressing block 7 relative to the second end. The first end has a second ramp 81 (shown in fig. 3 and 4) that mates with the first ramp 71. The movable block 8 is used for applying force to the first inclined surface 71 through the second inclined surface 81 when moving to the first movable position, so that the pressing block 7 pushes the pressing plate 4 to move to the second position to apply force to the collecting bag 10 (as shown in fig. 2). The box cover 11 is configured to apply a force to the second end of the movable block 8 when the access opening 101 is closed, so as to push the movable block 8 to move to the first movable position.

In operation, as shown in fig. 2, the cover 11 is closed, and the cover 11 applies a force to the second end of the movable block 8 to move the movable block 8 to the first movable position. When the movable block 8 moves to the first movable position, force is applied to the pressing block 7 through the second inclined surface 81, so that the pressing block 7 pushes the pressing plate 4 to move to the second position, and pressure is applied to the collection bag 10, so that the collection bag 10 is flattened.

Further, the aforementioned position adjusting mechanism 6 may further include a first elastic member, such as a spring. The first elastic member is used to apply a force to the movable block 8 to move the movable block 8 to a second movable position in which the second end protrudes out of the access opening 101 when the lid 11 is opened. Because the second end of the movable block 8 extends out of the pick-and-place opening 101, the box cover 11 can apply force to the second end of the movable block 8 when the pick-and-place opening 101 is closed, so that the movable block 8 is retracted into the first movable position in the box body 1, and the operation is more convenient.

Preferably, as shown in fig. 1, one end of the cover 11 may be connected to the case 1 through a hinge to be opened and closed with respect to the case 1 in a rotatable manner. To facilitate the lid 11 exerting a force on the second end of the movable block 8 during the pivoting closure, the second end of the movable block 8 may have an arcuate chamfer.

Further, as shown in fig. 1, a first protrusion 111 may be disposed on the cover 11 at a position corresponding to the second end of the movable block 8. When the access opening 101 is closed, the cover 11 applies a force to the second end of the movable block 8 through the first protrusion 111, so that the movable block 8 moves to the first movable position. In this example, the first protrusion 111 is provided to improve the strength of the cover 11 and reduce the wear on the cover 11 during the pressing process.

Preferably, the first protrusion 111 is detachable with respect to the cover 11, such as by screwing, so that the first protrusion 111 can be replaced again after being worn after long-term use, thereby reducing the maintenance cost.

Further, as shown in fig. 1 to 3, the position adjustment mechanism 6 of the present invention may further include a fixing plate 15. The fixing plate 15 is provided above the pressure plate 4 with a space from the pressure plate 4. The lower side of the fixing plate 15 is provided with a slide rail 9. Wherein, the movable block 8 is slidably disposed in the slide rail 9 to move along the slide rail 9 to the aforementioned first movable position and second movable position. In this example, the accuracy and stability of the movement of the movable block 8 can be improved by the provision of the slide rail 9.

Further, as shown in fig. 3, in the example where the aforementioned position adjustment mechanism 6 includes the first elastic member, a supporting block 91 may be provided on the lower side of the fixed plate 15. The side of the movable block 8 may be provided with a second protrusion 82. The second projection 82 is opposite to the supporting block 91. The aforementioned first elastic member is disposed between the second protrusion 82 and the supporting block 91.

Further, as shown in fig. 3, the number of the second protrusions 82 may be two. One second tab 82 is located on a first side of the movable block 8 and the other second tab 82 is located on a second side of the movable block 8. The first side part and the second side part are back. The number of the supporting blocks 91 and the number of the first elastic elements are two, and the supporting blocks correspond to the second protrusions 82 one by one, so that the movable block 8 is kept stressed in a balanced manner during the movement process.

Further, as shown in fig. 3, a first support post 911 may be disposed on a side of the support block 91 opposite to the second protrusion 82. A second support column 821 may be provided on a side of the second protrusion 82 opposite to the support block 91. One end of the first elastic member is sleeved on the first support column 911, and the other end is sleeved on the second support column 821. In this example, the first elastic member is restrained by the first support post 911 and the second support post 821, and the first elastic member is prevented from being released from between the support block 91 and the second protrusion 82.

Further, as shown in fig. 1, in the example where the number of the collection bag accommodation mechanisms 2 is two or more, the collection bag accommodation mechanisms 2 are arranged in order from the top to the bottom. Wherein, two adjacent collection bag containing mechanisms 2 are respectively a first collection bag containing mechanism 21 and a second collection bag containing mechanism 22. The first collection bag containing mechanism 21 is positioned above the second collection bag containing mechanism 22. The aforementioned position adjusting mechanism 6 includes a first position adjusting mechanism 61 corresponding to the first collecting bag containing mechanism 21 and a second position adjusting mechanism 62 corresponding to the second collecting bag containing mechanism 22. The second position adjusting mechanism 62 is located between the first collecting bag accommodating mechanism 21 and the second collecting bag accommodating mechanism 22, and the supporting plate 3 of the first collecting bag accommodating mechanism 21 and the fixing plate 15 of the second position adjusting mechanism 62 are the same plate, so that at least one plate can be saved, the cost can be reduced, and the space inside the box body 1 can be saved.

Further, as shown in fig. 5 and 6, the upper side of the pressing plate 4 may be provided with a limiting groove 41, and the pressing block 7 is inserted into the limiting groove 41 from above, so that the pressing block 7 and the pressing plate 4 can be assembled and disassembled more conveniently, and the pressing block 7 can be replaced in time when worn due to long-term use, so as to reduce the maintenance cost.

Of course, in an alternative example, the aforementioned pressing block 7 may also be fixed on the pressing plate 4, for example, the two pressing blocks are integrally formed.

Further, as shown in fig. 5 and 6, the upper side of the pressing plate 4 may be provided with a first limit projection 401 and a second limit projection 402. The first limiting protrusion 401 is opposite to the second limiting protrusion 402, and the limiting groove 41 is formed therebetween. In this example, the first limiting projection 401 and the second limiting projection 402 are provided to form the limiting groove 41 and enhance the strength of the pressing plate 4.

Further, as shown in fig. 5 and 6, the pressing block 7 is located at the middle position of the upper side of the pressing plate 4, so that when the pressing block 7 presses the collecting bag 10 below the pressing plate 4, the pressure at all positions of the collecting bag 10 can be more uniformly pressed, and the collecting bag 10 can be uniformly cooled in the low-temperature refrigerator.

Further, as shown in fig. 1, the position adjusting mechanism 6 may further include a second elastic member 12, such as a spring. The second elastic member 12 is used for providing a force for returning the pressing plate 4 to the first position, so as to achieve the technical effect of saving manpower. Specifically, when the box cover 11 is opened, the movable block 8 moves to a second movable position where the second end extends out of the taking and placing opening 101, and at the moment, the movable block 8 makes a movement space for the pressing block 7; then the second elastic member 12 pushes the pressing plate 4 and the pressing block 7 to move upwards, so as to increase the height of the accommodating gap 5 between the pressing plate 4 and the supporting plate 3, and facilitate the user to take and place the collecting bag 10 in the accommodating gap 5.

Further, as shown in fig. 1 and fig. 2, the position adjusting mechanism 6 may further include a limiting block 13. Stopper 13 sets up between clamp plate 4 and backup pad 3 for the degree of depth of bag 10 is gathered in the 4 pressfitting of restriction clamp plate, prevents that the degree of depth of bag 10 is gathered in the 4 pressfitting of clamp plate is too deep and lead to gathering bag 10 and break, so can play the function to the safety protection of gathering bag 10.

Further, as shown in FIGS. 1 and 2, the cord blood stem cell cryopreservation tank 100 of the present invention may further comprise a guide column 14. The guide posts 14 are fixedly connected with the support plate 3 and penetrate through the pressure plate 4, and the pressure plate 4 can move along the guide posts 14. In this example, since the guide posts 14 guide the movement of the platen 4, the movement accuracy and the movement stability of the platen 4 can be improved.

Both the guide posts 14 and the support plate 3 can be fixedly connected by fixing pins or the like.

In a specific application example, as shown in fig. 1 and 2, the number of the guide posts 14 may be four, and the guide posts are arranged at four corners of the support plate 3 in a one-to-one correspondence manner, so as to further improve the movement accuracy and the movement stability of the pressure plate 4.

As shown in fig. 1 and fig. 2, in the example that the position adjusting mechanism 6 includes the limiting block 13, the limiting block 13 may be sleeved on the guiding post 14, so that the limiting block 13 itself may be limited, and the limiting block 13 is prevented from being released from between the pressing plate 4 and the supporting plate 3.

Further, as shown in fig. 1 and 2, in the above-mentioned example where the number of the collection bag housing mechanisms 2 is two or more, each of the collection bag housing mechanisms 2 shares each of the guide posts 14, so that the number of the guide posts 14 can be reduced and the cost can be reduced, and each of the collection bag housing mechanisms 2 can be integrated into one body, so that the structure of the entire storage box can be more stable.

Here, it should be noted that: in the case of no conflict, a person skilled in the art may combine the related technical features in the above examples according to actual situations to achieve corresponding technical effects, and details of various combining situations are not described herein.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A cord blood stem cell cryopreservation box for cryopreservation of cord blood stem cells placed in a collection bag (10), comprising:

the box body (1) is provided with a box cover (11) and a taking and placing opening (101) positioned on the side face, and the box cover (11) can be opened and closed relative to the taking and placing opening (101);

the collecting bag accommodating mechanism (2) comprises a supporting plate (3) and a pressing plate (4) which are arranged in parallel, the supporting plate (3) and the pressing plate (4) are both positioned in the box body (1), and the pressing plate (4) is positioned above the supporting plate (3); the pressing plate (4) can move relative to the supporting plate (3) so as to form an accommodating gap (5) for placing a collection bag (10) between the pressing plate and the supporting plate when the pressing plate moves to the first position, and a gap opening of the accommodating gap (5) is opposite to the taking and placing opening (101); and pressing the collection bag (10) when moved to the second position; and

a position adjustment mechanism (6) for cooperating with the lid (11) to position the pressure plate (4) in the first position when the lid (11) is open; and to bring the pressure plate (4) into the second position when the lid (11) is closed;

wherein the position adjusting mechanism (6) comprises a pressing block (7) and a movable block (8);

a limiting groove (41) is formed in the upper side of the pressing plate (4), and the pressing block (7) is inserted into the limiting groove (41) from the upper side; one end of the pressing block (7) departing from the pressing plate (4) is provided with a first inclined surface (71);

the movable block (8) is arranged in the box body (1) and is positioned above the pressing plate (4); the movable block (8) is provided with a first end and a second end which are opposite, and the first end is close to the pressing block (7) relative to the second end; the first end is provided with a second inclined surface (81) matched with the first inclined surface (71), and the movable block (8) is used for applying force to the first inclined surface (71) through the second inclined surface (81) when moving to a first movable position so that the pressing block (7) pushes the pressing plate (4) to move to a second position; the box cover (11) is used for applying force to the second end of the movable block (8) when being closed so as to push the movable block (8) to move to the first movable position;

the number of the collecting bag accommodating mechanisms (2) is more than two, and the collecting bag accommodating mechanisms are sequentially arranged from top to bottom; the number of the position adjusting mechanisms (6) is equal to that of the collecting bag containing mechanisms (2), and the position adjusting mechanisms are arranged in a one-to-one correspondence manner.