CN102550835B - Microbe feed additive and preparation method thereof - Google Patents
Microbe feed additive and preparation method thereof Download PDFInfo
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- CN102550835B CN102550835B CN2012100306041A CN201210030604A CN102550835B CN 102550835 B CN102550835 B CN 102550835B CN 2012100306041 A CN2012100306041 A CN 2012100306041A CN 201210030604 A CN201210030604 A CN 201210030604A CN 102550835 B CN102550835 B CN 102550835B
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Abstract
The invention discloses a microbe feed additive which contains the following microorganisms in percentage by weight: 10% to 30% of Bacillus subtilis, 20% to 40% of Aspergillus niger, 10% to 15% of yeast, 26% to 32% of lactobacilli, and 10% to 16% of bifidobacterium. The invention also relates to a preparation method of the additive. The preparation method comprises the following steps: (1), preparing a culture medium; (2), performing enlargement culture of the bacterial strains respectively, performing solid-state fermentation, air-drying, grinding, and proportionally mixing to obtain a finished microbial product; and (3), mixing the finished microbial product with auxiliary ingredients, air-drying and sieving to obtain the microbe feed additive/premix. The microbe feed additive can improve nonspecific immunity of animals and enhance disease resistance of animals.
Description
Technical field
The present invention relates to a kind of additive for microbe feedstuff and preparation method, belong to the cultivation field.
Background technology
Feed addictive refers to a small amount of or micro substance that adds in Feed Manufacturing processing, use procedure, in feed consumption seldom but effect significantly.Feed addictive is the raw material that modern feed industry must use, and to strengthening the basal feed nutritive value, improves breeding performonce fo animals, guarantees animal health, saves feed cost, and improving the aspect such as livestock products quality has obvious effect.
Additive for microbe feedstuff is the microbial product of one or more fungus strains in a kind of replacement or the balance animal ecosystem.Say narrowly, it is a kind ofly can excite the breeding of self probiotics to increase, and resists simultaneously the microbial product of harmful fungus strain growth.Wherein contain a large amount of beneficial bacteriums, complex enzyme, chelating peptide takes off mould dose etc., after entering in the livestock and poultry body as feed, can breed rapidly, drop on the one hand in the metabolin of bacterial classification and enterogenous endotoxin, suppressed the growth of other harmful flora, formed on the other hand normal microorganism species in host, be the synthetic main vitamin of host, nutrition be provided and stop the invasion of pathogenic bacteria.Therefore, synthetic a kind of additive for microbe feedstuff with above-mentioned effect increases the synergy between each microorganism, will improve greatly culture benefit.
Summary of the invention
The purpose of this invention is to provide and improve animal nonspecific immunity power, strengthen the feed addictive of resisting stress capability.
For achieving the above object, various microorganism fungus kind percentage by weights of the present invention are:
Bacillus subtilis 10-30%, black-koji mould 20-40%, saccharomycete 10-15%, lactic acid bacteria 26-32%, Bifidobacterium 10-16%.
Preferably, the percentage by weight of microorganism is:
Bacillus subtilis 15-25%, black-koji mould 25-35%, saccharomycete 10-13%, lactic acid bacteria 26-30%, Bifidobacterium 11-15%.
Further preferred, bacillus subtilis 20%, black-koji mould 28%, saccharomycete 12%, lactic acid bacteria 28%, Bifidobacterium 12%.
A kind of production method of microbial feed additive/premixed material is characterized in that, the method comprises the steps: (1) preparation culture medium; (2) bacillus subtilis, black-koji mould, saccharomycete, lactic acid bacteria, Bifidobacterium original seed are enlarged respectively cultivation, then air-dry after solid fermentation, pulverizing, be mixed with the microorganism finished product in proportion; (3) the microorganism finished product is mixed with batching, sieve after air-dry, namely obtain microbial feed additive/premixed material.
NM in Bacteria Culture and detection method specification in the present invention, be the method for this area routine.
Bacillus subtilis
One-level is cultivated
Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.02%, yeast extract 0.05%, agar 3%, temperature is 28 ℃ ~ 35 ℃, incubation time 3 ~ 5;
Secondary is cultivated:
The one-level culture medium removes agar, and temperature is 28 ℃ ~ 35 ℃, incubation time 5 ~ 7 days;
Three grades of cultivations
Described medium component is by percentage to the quality: corn flour 10-15%, dregs of beans 25-30%, wheat bran 20-35%, pomace 10-20%, peptone 1-10%, dipotassium hydrogen phosphate 0.05-1%, magnesium sulfate 0.05-1%, calcium carbonate 0.05-1%, Tween-80 0.05-1%; Temperature is 28 ℃ ~ 35 ℃, incubation time 5 ~ 7 days.
After testing, total number of bacteria reaches 2 * 10
8Individual/more than gram.
Lactic acid bacteria
Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.02%, yeast extract 0.05%, agar 3%, temperature 28-32 ℃, time 2-5 days.
Secondary is cultivated:
The one-level culture medium removes agar, and temperature 28-32 ℃, time 2-5 days.
Three grades of cultivations
Described medium component is by percentage to the quality: corn flour 10-15%, dregs of beans 25-30%, wheat bran 20-35%, pomace 10-20%, peptone 1-10%, dipotassium hydrogen phosphate 0.05-1%, magnesium sulfate 0.05-1%, calcium carbonate 0.05-1%, Tween-80 0.05-1%; Temperature 28-32 ℃, time 2-5 days.
After testing, total number of bacteria reaches 2 * 10
8Individual/more than gram.
Bifidobacterium
One-level is cultivated
Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.02%, yeast extract 0.05%, agar 3%, temperature is 28 ℃ ~ 35 ℃, incubation time 3 ~ 5;
Secondary is cultivated:
The one-level culture medium removes agar, and temperature is 28 ℃ ~ 35 ℃, incubation time 5 ~ 7 days;
Three grades of cultivations
Described medium component is by percentage to the quality: corn flour 10-15%, dregs of beans 25-30%, wheat bran 20-35%, pomace 10-20%, peptone 1-10%, dipotassium hydrogen phosphate 0.05-1%, magnesium sulfate 0.05-1%, calcium carbonate 0.05-1%, Tween-80 0.05-1%; Temperature is 28 ℃ ~ 35 ℃, incubation time 5 ~ 7 days.
After testing, total number of bacteria reaches 2 * 10
8Individual/more than gram.
Black-koji mould
One-level is cultivated
Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.02%, yeast extract 0.05%, agar 3%, temperature is 33-37 ℃, incubation time 3 ~ 5;
Secondary is cultivated:
The one-level culture medium removes agar, and temperature is 33-37 ℃, incubation time 5 ~ 7 days;
Three grades of cultivations
Described medium component is by percentage to the quality: corn flour 10-15%, dregs of beans 25-30%, wheat bran 20-35%, pomace 10-20%, peptone 1-10%, dipotassium hydrogen phosphate 0.05-1%, magnesium sulfate 0.05-1%, calcium carbonate 0.05-1%, Tween-80 0.05-1%; PH 7-7.2, temperature is 33-37 ℃, incubation time 5 ~ 7 days.
After testing, total number of bacteria reaches 2 * 10
8Individual/more than gram.
Saccharomycete
One-level is cultivated
Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.02%, yeast extract 0.05%, agar 3%, temperature is 28 ℃ ~ 35 ℃, incubation time 3 ~ 5;
Secondary is cultivated:
The one-level culture medium removes agar, and temperature is 28 ℃ ~ 35 ℃, incubation time 5 ~ 7 days;
Three grades of cultivations
Described medium component is by percentage to the quality: corn flour 10-15%, dregs of beans 25-30%, wheat bran 20-35%, pomace 10-20%, peptone 1-10%, dipotassium hydrogen phosphate 0.05-1%, magnesium sulfate 0.05-1%, calcium carbonate 0.05-1%, Tween-80 0.05-1%; Temperature is 28 ℃ ~ 35 ℃, incubation time 5 ~ 7 days.
After testing, total number of bacteria reaches 2 * 10
8Individual/more than gram.
Described batching comprises zeolite powder 20-40%, steamed bone meal 20-40%, ferrous sulfate 4-8%, sodium chloride 10-20%, zinc sulfate 5-8%, copper sulphate 3-8%, microorganism finished product 1-2%.
In described batching, every kilogram of feed can add vitamin A 40-60MIU, vitamin D
230-50 MIU, vitamin E 40-60MIU.
The present invention can improve animal nonspecific immunity power, strengthen resisting stress capability, activate growth of animal potential, improve production performance comprehensively, package solution animal products quality is low, the local flavor disappearance, grice diarrhoea, prevention of sow constipation, chicken and duck loose and watery stool are half congealed, and the problem that the feedstuff ANFs is many, digestibility and utilization is low provides the cheap simple and practical scheme of substitute antibiotics, enzyme preparation, arsenic preparation, probiotic.
Table 1 is after feed addictive of the present invention is fed, the contrast of each livestock and poultry illness rate.
Selection suffers from the pig of diarrhoea, each 40 of experimental group and control groups, and experimental group was fed 5 days with additive of the present invention, and the per kilogram feed adds additive 2g; Control group is fed with orfloxacin, and the per kilogram feed adds 3g.
Criterion: return to one's perfect health, produce effects; Diarrhoea obviously alleviates, and the number of times of suffering from diarrhoea every day is effectively less than 5 times; The diarrhoea number of times has no and alleviates, and is invalid.
| ? | n | Produce effects | Effectively | Invalid | Efficient |
| Control group | 40 | 26 | 9 | 5 | 87.25% |
| Experimental group | 40 | 31 | 7 | 2 | 95% |
As can be seen from Table 1, additive of the present invention with the orfloxacin group relatively has better effect.Illustrate that additive of the present invention has splendid effect to conditioning animal diarrhoea.
The specific embodiment
Embodiment 1
(1) preparation culture medium;
Percentage by weight is:
Sucrose 1.5%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.02%, yeast extract 0.05%, agar 3%;
Percentage by weight is:
Corn flour 10-15%, dregs of beans 25-30%, wheat bran 20-35%, pomace 10-20%, peptone 1-10%, dipotassium hydrogen phosphate 0.05-1%, magnesium sulfate 0.05-1%, calcium carbonate 0.05-1%, Tween-80 0.05-1%;
(2) bacillus subtilis, black-koji mould, saccharomycete, lactic acid bacteria, Bifidobacterium original seed are enlarged respectively cultivation, then air-dry after solid fermentation, pulverizing, be mixed with the microorganism finished product in proportion.
Embodiment 2
Following column weight amount percentage preparing microorganism composition:
Bacillus subtilis 25%, black-koji mould 25%, saccharomycete 10%, lactic acid bacteria 26%, Bifidobacterium 14%.
Add batching, after mixing, the weight ratio of feed is: zeolite powder 40%, steamed bone meal 37%, ferrous sulfate 4%, sodium chloride 10%, zinc sulfate 5%, copper sulphate 3%, microorganism finished product 1%.In batching, every kilogram of feed adds vitamin A 40MIU, vitamin D
230 MIU, vitamin E 40MIU.
Embodiment 3
Following column weight amount percentage preparing microorganism composition:
Bacillus subtilis 18%, black-koji mould 30%, saccharomycete 12%, lactic acid bacteria 27%, Bifidobacterium 13%.
Add batching, after mixing, the weight ratio of feed is: zeolite powder 24%, steamed bone meal 30%, ferrous sulfate 8%, sodium chloride 20%, zinc sulfate 8%, copper sulphate 8%, microorganism finished product 2%.In batching, every kilogram of feed adds vitamin A 60MIU, vitamin D
250 MIU, vitamin E 60MIU.
Embodiment 4
Following column weight amount percentage preparing microorganism composition:
Bacillus subtilis 17%, black-koji mould 33%, saccharomycete 10%, lactic acid bacteria 29%, Bifidobacterium 11%.
Add batching, after mixing, the weight ratio of feed is: zeolite powder 40%, steamed bone meal 25%, ferrous sulfate 6%, sodium chloride 15%, zinc sulfate 6%, copper sulphate 7%, microorganism finished product 1%.In batching, every kilogram of feed contains vitamin A 50MIU, vitamin D
240 MIU, vitamin E 50MIU.
Embodiment 5
Following column weight amount percentage preparing microorganism composition:
Bacillus subtilis 18%, black-koji mould 25%, saccharomycete 12%, lactic acid bacteria 30%, Bifidobacterium 15%.
Add batching, after mixing, the weight ratio of feed is: zeolite powder 32%, steamed bone meal 31%, ferrous sulfate 5%, sodium chloride 18%, zinc sulfate 7%, copper sulphate 5%, microorganism finished product 2%.In batching, every kilogram of feed adds vitamin A 55MIU, vitamin D
245 MIU, vitamin E 54MIU.
Embodiment 6
Following column weight amount percentage preparing microorganism composition:
Bacillus subtilis 20%, black-koji mould 28%, saccharomycete 12%, lactic acid bacteria 28%, Bifidobacterium 12%.
Add batching, after mixing, the weight ratio of feed is: zeolite powder 34%, steamed bone meal 31%, ferrous sulfate 7%, sodium chloride 17%, zinc sulfate 6%, copper sulphate 4%, microorganism finished product 1%.In batching, every kilogram of feed contains vitamin A 58MIU, vitamin D
235 MIU, vitamin E 45MIU.
Embodiment 7
Following column weight amount percentage preparing microorganism composition:
Bacillus subtilis 20%, black-koji mould 20%, saccharomycete 15%, lactic acid bacteria 30%, Bifidobacterium 15%.
Add batching, after mixing, the weight ratio of feed is: zeolite powder 28%, steamed bone meal 40%, ferrous sulfate 4%, sodium chloride 10%, zinc sulfate 8%, copper sulphate 8%, microorganism finished product 2%.In batching, every kilogram of feed adds vitamin A 40MIU, vitamin D
230 MIU, vitamin E 40MIU.
Claims (1)
1. an additive for microbe feedstuff, is characterized in that, the preparation method comprises the steps:
(1) preparation culture medium;
(2) bacillus subtilis, black-koji mould, saccharomycete, lactic acid bacteria, Bifidobacterium original seed are enlarged respectively cultivation, then air-dry after solid fermentation, pulverizing, be mixed with the microorganism finished product in proportion;
Described ratio is: bacillus subtilis 10-30%, black-koji mould 20-40%, saccharomycete 10-15%, lactic acid bacteria 26-32%, Bifidobacterium 10-16%;
(3) the microorganism finished product is mixed with batching, sieve after air-dry, and get final product;
Described microorganism finished product mixes with batching, and mixed feed percentage by weight is: zeolite powder 20-40%, steamed bone meal 20-40%, ferrous sulfate 4-8%, sodium chloride 10-20%, zinc sulfate 5-8%, copper sulphate 3-8%, microorganism finished product 1-2%.
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| CN102550835B true CN102550835B (en) | 2013-06-12 |
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| CN103421712B (en) * | 2013-07-29 | 2016-03-30 | 内蒙古和美科盛生物技术有限公司 | For animal bifidobacteria and the subtilis of fodder additives probiotic solid fermentation |
| CN103749942A (en) * | 2013-12-31 | 2014-04-30 | 陕西万源生物农业科技有限公司 | Compound probiotics for animal breeding |
| CN105166416A (en) * | 2015-10-13 | 2015-12-23 | 武汉新兴地生物技术发展有限公司 | Compound microorganism feed additive and preparation method thereof |
| CN106666178A (en) * | 2015-11-06 | 2017-05-17 | 丹阳市尚德生物科技有限公司 | Microbial additive for poultry feed and preparation method of microbial additive |
| CN106135664A (en) * | 2016-04-01 | 2016-11-23 | 李建秋 | A kind of for microbial ecological agent treating and preventing disease of alimentary tract of livestock husbandry and birds and its production and use |
| CN105994940B (en) * | 2016-05-23 | 2020-07-07 | 西北农林科技大学 | Bioactive protein feed and preparation method thereof |
| CN106983032A (en) * | 2017-03-13 | 2017-07-28 | 苏州顶裕生物科技有限公司 | A kind of microorganism compound feed additive and preparation method thereof |
| CN109007246A (en) * | 2018-07-25 | 2018-12-18 | 佛山科学技术学院 | A kind of composite microbial feed additive and preparation method thereof |
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| CN101779731B (en) * | 2010-03-12 | 2013-07-03 | 韶关市颐林泉生物科技有限公司 | Feed additive |
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