CN102539752B - Oral fluid diagnosing instrument - Google Patents
Oral fluid diagnosing instrument Download PDFInfo
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- CN102539752B CN102539752B CN201010593654.1A CN201010593654A CN102539752B CN 102539752 B CN102539752 B CN 102539752B CN 201010593654 A CN201010593654 A CN 201010593654A CN 102539752 B CN102539752 B CN 102539752B
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- oral liquid
- diagnostic instrument
- nanoporous matrix
- test paper
- connection strap
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Abstract
The invention relates to an oral fluid diagnosing instrument comprising a detecting test paper and a nano-porous substrate which are wrapped in an outer shell, wherein the outer shell consists of an upper shell, a middle shell and a lower shell; wherein a through hole and an embedding groove for placing the detecting test paper are arranged on the middle shell; a spring is arranged on the lower shell; an ejection block is fixed on the spring; a locating pin is extruded on the ejection block; connecting strips are arranged at the two ends of the detecting test paper; a locating hole is arranged on the nano-porous matrix; the nano-porous matrix is placed on the ejection block; a connecting strip is arranged on the nano-porous substrate; a blocking strip is arranged on the connecting strip; and a push hand is fixed on the side face of the nano-porous substrate. The oral fluid diagnosing instrument is simple in structure and convenient for operation, has the advantages that different detecting test paper can be replaced to ensure that any type of antigen and antibody can be detected, and the sample collecting part is effectively protected from external pollution and can be widely used for early-stage self-test of various infectious diseases and hospitals.
Description
Technical field
The present invention relates to a kind of diagnostic instrments, relate in particular to a kind of diagnostic instrument for oral liquid.
Background technology
Communicable disease is one of main killer of the mankind.Acquired immune deficiency syndrome (AIDS), various types of hepatitis, bird flu etc., do not threatening the mankind's health and lives all the time.For this serious infectious diseases, early stage diagnosis is particularly important.Early stage making a definite diagnosis is conducive to patient and given treatment to as early as possible, is also conducive to control transmission of disease.At present, to the still detection of " positive " of infectious disease " feminine gender ", main by the means of blood examination.But blood examination generally need to puncture person under inspection's skin, belong to a kind of wound inspection.It has not only brought misery to patient, and has increased the infected danger of medical worker.In contrast, collecting mouth cavity liquid (comprising saliva and mucous membrane of mouth transudate) is that a kind of " without wound " detects, and relatively simple, safety, and fast.Scientific circles and medical circle are just used mouth cavity liquid to carry out a large amount of development tests as detecting sample, and have successfully proved and can use mouth cavity liquid to detect effectively rapidly disease.
Detection for mouth cavity liquid (comprising saliva and mucous membrane of mouth transudate) is that a kind of " painless " detects, and it is simple to operate, quick.Scientific circles and medical circle are just used mouth cavity liquid to carry out a large amount of development tests as detecting sample, and have successfully proved and can use mouth cavity liquid to detect effectively rapidly disease.From physiological angle, the material in blood (comprising antiviral antibody, antigen etc.) can be diffused in oral cavity and be gone by mucous membrane of mouth and salivary gland.So in theory, any material in blood can be detected in oral cavity, and its content exists certain association.Medical circle is filled with unbounded confidence to this, thinks and how long does not need, and the inspection of oral fluid will replace blood test completely.Patient goes to a hospital to see a doctor, and again without bearing affliction, has drawn blood.In April, 2008, American Studies personnel have drawn human saliva's protein picture group.In the paper of publication, they sketch out the prospect of following patient " spitting ptysis is seen a doctor ".Researchist believes, saliva detection is expected to become the blood test of " modified version ", and finally replaces.FDA (Food and Drug Adminstration) (FDA) approval of the ELISA that the saliva that take is sample determination HIV antibody, Western blot (WB) reagent.For the clinical practice of sialoprotein matter research, researchist is generally good.Saliva is seen a doctor not only convenient, and price is also inexpensive.Except the detection of some common transmittable diseases, doctor even can pass through saliva in the future, tells whether patient suffers from heart disease or cancer.Because saliva test is very convenient, it can help doctor in the ill early detection disease of patient.
U.S. Kerry Pood biomedical (Calypte Biomecical Co.) is a company that is engaged in oral fluid disease detection.The said firm releases a kind of saliva of buccal cavity acquired immune deficiency syndrome (AIDS) fast detecting equipment in China.Registration and the selling license of this method and equipment Yi Huo state food and drug administration.The above-mentioned saliva of buccal cavity detection method of Kerry Pood company can detect HIV-1 or HIV-2 virus in 30 minutes, and levels of precision is suitable with accredited laboratory of FDA (Food and Drug Adminstration) standard blood testing standard.According to the data of introducing of Kerry Pood company, above-mentioned fast detecting product can be applied by medical testing agency, as the get the Green Light part of the HIV antibody test integrated application used of multiple fast detecting and other.But the contained parts of this kit are more, running program is complicated.
Acquired immune deficiency syndrome (AIDS) and hepatitis B oral fluid fast detecting product that U.S. Ao Luoxiuer (Orasure) company produces are widely used at U.S.'s various big hospital and Center for Disease Control (CDC).The saliva acquired immune deficiency syndrome (AIDS) checkout equipment that he provides is an integrated disposable use instrument.Testing result was read in 20 minutes.Rate of accuracy reached to 99.8%.Be different from kit, this instrument is a kind of collection that collects mouth cavity liquid sample, analyzes and the results are shown in a brand-new instrument all over the body.
The patent of Ao Luoxiuer (Orasure) company and domestic patent similarly, application number is respectively 200710169737,200720086186,200710169735 disclosed technology paths and has following problem: (1) sample collection part all adopts micron order capillary matrix, and that micron order capillary matrix obtains voidage is generally larger.Therefore, can be detained more mouth cavity liquid in sample collection part, reduce the efficiency of sample collection.In addition, micron-sized aperture is still larger, and some minute impurities in sample can be passed through, and the Test paper after flowing to gets on, and enters the Children's Film Studio of China and rings testing result; (2) sample collection is partly directly exposed to outside plastic casing, has so both been unfavorable for clean operation, and has increased again the length of whole detecting device.
Summary of the invention
In order to overcome above defect, the technical problem to be solved in the present invention is: a kind of diagnostic instrument for oral liquid quick, easy and simple to handle is provided.
In order to overcome the defect existing in background technology, the technical solution adopted for the present invention to solve the technical problems is: a kind of diagnostic instrument for oral liquid, comprise Test paper, also be provided with nanoporous matrix, described Test paper, nanoporous matrix is wrapped in a shell, described shell is by upper casing, mesochite, lower casing forms, on described mesochite, have through hole, place the embedded groove of Test paper, on described lower casing, be provided with spring, on described spring, be fixed with angled slide block, on described angled slide block, be extruded with register pin, the two ends of described Test paper are provided with connection strap, in described nanoporous matrix, have pilot hole, described nanoporous matrix is placed on angled slide block, in described nanoporous matrix, be placed with connection strap, on described connection strap, be provided with blocking-up bar, the side of described nanoporous matrix is fixed with pushing hands.
According to another preferred embodiment, diagnostic instrument for oral liquid further comprises the display window that is provided with venthole and inspections and examinations result on described upper casing.
According to another preferred embodiment, on the connection strap away from nanoporous matrix described in diagnostic instrument for oral liquid further comprises, be provided with absorption piece, one end of another connection strap is passed the through hole on mesochite and is attached on the bottom surface of mesochite.
According to another preferred embodiment, diagnostic instrument for oral liquid further comprises that described blocking-up bar includes one or more detersives and buffering agent.
According to another preferred embodiment, diagnostic instrument for oral liquid further comprises that described detersive contains bovine serum albumin(BSA), dissociative type deoxycholic acid and N-sodium lauroyl sarcosine.
According to another preferred embodiment, diagnostic instrument for oral liquid further comprises that described buffering agent is for regulating the pH value of mouth cavity liquid sample.
According to another preferred embodiment, diagnostic instrument for oral liquid further comprises that the aperture of described nanoporous matrix is 50-900 nanometer.
According to another preferred embodiment, diagnostic instrument for oral liquid further comprises that described nanoporous matrix made by one or more plastic materials.
According to another preferred embodiment, diagnostic instrument for oral liquid further comprises that described plastic material is high density polyethylene, polypropylene, teflon, nylon, polyester or polyethersulfone resin.
According to another preferred embodiment, diagnostic instrument for oral liquid further comprises and can be used in conjunction with dilution, accelerates the time of diagnosis.
The invention has the beneficial effects as follows: simple in structure; easy to operate; can quick collection and detect mouth cavity liquid; can detect various biomolecule contained in antigen, antibody and the human body of any type; and effectively protected sample collection part not to be subject to the damage and the distortion that in external contamination and transportation, likely produce; be applicable to large-scale production, can be widely used in early stage self check and the hospital of various infectious diseases.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is the structural representation of the preferred embodiments of the present invention;
Fig. 2 is the plan structure schematic diagram that nanoporous matrix of the present invention is not stretched out shell;
Fig. 3 is the plan structure schematic diagram that nanoporous matrix of the present invention is stretched out shell;
Fig. 4 is the plan structure schematic diagram of upper casing;
Fig. 5 is the plan structure schematic diagram of mesochite;
Fig. 6 is the plan structure schematic diagram of lower casing;
In figure: 1, Test paper, 2, nanoporous matrix, 3, shell, 3a, upper casing, 3b mesochite, 3c, lower casing, 4, blocking-up bar, 5, absorb piece, 6, display window, 8, pushing hands, 9, spring, 10, angled slide block, 11, embedded groove, 12, register pin, 13, connection strap, 14, through hole, 15, pilot hole, 16, connection strap, 17, venthole.
Embodiment
The present invention is further detailed explanation with preferred embodiment by reference to the accompanying drawings now.These accompanying drawings are the schematic diagram of simplification, basic structure of the present invention is only described in a schematic way, so it only show the formation relevant with the present invention.
As Fig. 1, Fig. 2, Fig. 3, Fig. 4, Fig. 5, shown in Fig. 6, a kind of diagnostic instrument for oral liquid, comprise Test paper 1, also be provided with nanoporous matrix 2, the sampling end of nanoporous matrix 2 can be designed to circle, oval, the shape of any applicable collection mouth cavity liquid such as rectangle, described Test paper 1, nanoporous matrix 2 is wrapped in a shell 3, shell 3 can be any shape and color, effectively protected nanoporous matrix 2 not to be subject to issuable distortion or damage in external contamination and transportation, 3 whiles of shell are as the handle of whole instrument, person under inspection can hold shell 3 the nanoporous matrix 2 of instrument is stretched in face, described shell 3 is by upper casing 3a, mesochite 3b, lower casing 3c forms, on described mesochite 3b, have through hole 14, place the embedded groove 11 of Test paper 1, on described lower casing 3c, be provided with spring 9, on described spring 9, be fixed with angled slide block 10, on described angled slide block 10, be extruded with register pin 12, in described nanoporous matrix 2, have pilot hole 15, described nanoporous matrix 2 is placed on angled slide block 10, in described nanoporous matrix 2, be placed with connection strap 16, on described connection strap 16, be provided with blocking-up bar 4, thereby the blocking-up reagent on blocking-up bar 4 can reduce the incidence that the generation of non-specific adsorption reduces " false positive ".The composition material of blocking-up bar 4 is including, but not limited to paper, cellulose, glass fibre or polyester.Blocking-up bar 4 can be designed to the form of semi-permeable diaphragm, thereby liquid can not get on from the Test paper 1 nanoporous matrix 2 that flows backwards back, and the side of described nanoporous matrix 2 is fixed with pushing hands 8.
As shown in Figure 4, on described upper casing 3a, set out the display window 6 of pore 17 and inspections and examinations result.
In addition, be provided with and absorb piece 5 on the described connection strap 13 away from nanoporous matrix 2, one end of another connection strap 13 is passed the through hole 14 on mesochite 3b and is attached on the bottom surface of mesochite 3b.
Further, blocking-up bar 4 includes one or more detersives and buffering agent, and wherein detersive contains but is not limited to bovine serum albumin(BSA), dissociative type deoxycholic acid and N-sodium lauroyl sarcosine, and buffering agent is for regulating the pH value of mouth cavity liquid sample.
In the present embodiment, the aperture of nanoporous matrix 2 is 50-900 nanometer, and therefore the voidage of whole sample significantly reduces, and has reduced the loss of mouth cavity liquid sample, and nano aperture contributes to filter out the minute impurities in mouth cavity liquid sample, play and purify sample, reduce the effect that detects error.This nanoporous matrix 2 is made by one or more plastic materials.Plastic material is including, but not limited to high density polyethylene, polypropylene, teflon, nylon, polyester or polyethersulfone resin.
Because some person under inspection's mouth cavity liquid viscosity is too large, cause the mouth cavity liquid of collecting cannot be sent to by capillarity the surveyed area of Test paper 1, or transfer rate causes whole diagnostic procedure consuming time long too slowly, now can be used in conjunction with dilution, accelerate the time of diagnosis.
When using this instrument, person under inspection moves pushing hands 8, nanoporous matrix 2 is and then mobile, after moving a certain distance, spring 9 on lower casing 3c makes the register pin 12 on angled slide block 10 snap in the pilot hole 15 in nanoporous matrix 2, the connection strap 16 of nanoporous matrix 2 one end is connected with the connection strap 13 being attached on mesochite 3b bottom surface simultaneously, nanoporous matrix 2 is inserted in mouth, then by two rows tooth, be contained in the mouth the regular hour or person under inspection contacts the sampling end of nanoporous matrix 2 with mucous membrane of mouth, and scraping back and forth, collect mucous membrane transudate.When person under inspection's mouth cavity liquid viscosity is too large, now person under inspection can insert a period of time in dilution by nanoporous matrix 2, the sampling end of nanoporous matrix 2 has absorbed after mouth cavity liquid or the mixing material after diluted, liquid flows towards the other end by capillarity, the impurity particle existing in sample etc. is filtered out by nano-porous structure, follow through connection strap 16, blocking-up bar 4, thereby the blocking-up reagent on blocking-up bar 4 can reduce the incidence that the generation of non-specific adsorption reduces " false positive ".After blocking-up bar 4, then liquid sample starts to flow to connection strap 13 and then to Test paper 1, flows, and has an effect, and produce a signal that can be detected when liquid flows through Test paper 1 with the reagent being fixed on Test paper 1.In general, this signal can be coloured lines.Whether person under inspection and medical worker can observe lines by display window 6 and exist, thereby determine " feminine gender " or " positive ".Final liquid flows to the end of connection strap 13, is absorbed piece 5 and absorbs and locking.Because register pin 12 snaps in the pilot hole 15 in nanoporous matrix 2, nanoporous matrix 2 can not be return in shell 3, represent to use, give person under inspection with prompting, prevent cross-infection.
This instrument can disposable detection many kinds of substance, and this material is including, but not limited to antigen and the antibody of the various diseases such as aids antibody, various types of hepatitis virus antibody, various types of hepatitis antigen, smallpox antibody, syphilis antibody.This material can be also variously to exist molecule in human body including, but not limited to glucose, transaminase, and cholesterol, lecithin, cocaine, nicotine, various hormone, can plant growth factor etc.The precision of this instrument diagnoses is greater than 99.7%, can be slightly different according to different Test papers used.As complete commodity, this instrument can with together with its aid, as a diagnosis box, sell.These aids, including, but not limited to damping fluid, are placed the shelf of buffering medicine bottle, instructions, teaching audio and video files etc.
The above-mentioned foundation desirable embodiment of the present invention of take is enlightenment, and by above-mentioned description, relevant staff can, within not departing from the scope of this invention technological thought, carry out various change and modification completely.The technical scope of this invention is not limited to the content on instructions, must determine its technical scope according to claim scope.
Claims (9)
1. a diagnostic instrument for oral liquid, comprise Test paper, it is characterized in that: be also provided with nanoporous matrix, described Test paper, nanoporous matrix is wrapped in a shell, described shell is by upper casing, mesochite, lower casing forms, on described mesochite, have through hole, place the embedded groove of Test paper, on described lower casing, be provided with spring, on described spring, be fixed with angled slide block, on described angled slide block, be extruded with register pin, the two ends of described Test paper are provided with the first connection strap, in described nanoporous matrix, have pilot hole, described nanoporous matrix is placed on angled slide block, in described nanoporous matrix, be placed with the second connection strap, on described the second connection strap, be provided with blocking-up bar, the side of described nanoporous matrix is fixed with pushing hands.
2. the diagnostic instrument for oral liquid as described in claim 1, is characterized in that: the display window that is provided with venthole and inspections and examinations result on described upper casing.
3. the diagnostic instrument for oral liquid as described in claim 1, is characterized in that: on described the first connection strap away from nanoporous matrix, be provided with absorption piece, one end of the first connection strap is passed the through hole on mesochite and is attached on the bottom surface of mesochite.
4. the diagnostic instrument for oral liquid as described in claim 1, is characterized in that: described blocking-up bar includes one or more detersives and buffering agent.
5. the diagnostic instrument for oral liquid as described in claim 4, is characterized in that: described detersive contains bovine serum albumin(BSA), dissociative type deoxycholic acid and N-sodium lauroyl sarcosine.
6. the diagnostic instrument for oral liquid as described in claim 4, is characterized in that: described buffering agent is for regulating the pH value of mouth cavity liquid sample.
7. the diagnostic instrument for oral liquid as described in claim 1, is characterized in that: the aperture of described nanoporous matrix is 50-900 nanometer.
8. the diagnostic instrument for oral liquid as described in claim 1, is characterized in that: described nanoporous matrix is made by one or more plastic materials.
9. the diagnostic instrument for oral liquid as described in claim 8, is characterized in that: described plastic material is high density polyethylene, polypropylene, teflon, nylon, polyester or polyethersulfone resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010593654.1A CN102539752B (en) | 2010-12-17 | 2010-12-17 | Oral fluid diagnosing instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010593654.1A CN102539752B (en) | 2010-12-17 | 2010-12-17 | Oral fluid diagnosing instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102539752A CN102539752A (en) | 2012-07-04 |
| CN102539752B true CN102539752B (en) | 2014-09-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010593654.1A Expired - Fee Related CN102539752B (en) | 2010-12-17 | 2010-12-17 | Oral fluid diagnosing instrument |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105223040A (en) * | 2015-09-30 | 2016-01-06 | 中山大学中山眼科中心 | A kind of method of getting tear |
Citations (5)
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| US6365417B1 (en) * | 2000-02-09 | 2002-04-02 | A-Fem Medical Corporation | Collection device for lateral flow chromatography |
| CN1760672A (en) * | 2004-10-12 | 2006-04-19 | 艾康生物技术(杭州)有限公司 | Device for detecting sample, method for detecting sample, and modules for collecting and detecting sample |
| EP1301782B1 (en) * | 2000-07-06 | 2007-08-22 | Varian, Inc. | Saliva testing and confirmation device |
| CN101238995A (en) * | 2007-02-05 | 2008-08-13 | 深圳市赛茵斯爱速医疗科技有限公司 | Sample collecting device |
| CN101266243A (en) * | 2008-01-31 | 2008-09-17 | 程柯 | Instrument for collecting and rapidly detecting oral liquid |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1955341B (en) * | 2005-10-25 | 2011-09-07 | 李碧洁 | Copper antioxidation agent suitable for multiple high temp leadless welding |
| CN201955341U (en) * | 2010-12-17 | 2011-08-31 | 苏州万木春生物技术有限公司 | Diagnostic instrument for oral liquid |
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2010
- 2010-12-17 CN CN201010593654.1A patent/CN102539752B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6365417B1 (en) * | 2000-02-09 | 2002-04-02 | A-Fem Medical Corporation | Collection device for lateral flow chromatography |
| EP1301782B1 (en) * | 2000-07-06 | 2007-08-22 | Varian, Inc. | Saliva testing and confirmation device |
| CN1760672A (en) * | 2004-10-12 | 2006-04-19 | 艾康生物技术(杭州)有限公司 | Device for detecting sample, method for detecting sample, and modules for collecting and detecting sample |
| CN101238995A (en) * | 2007-02-05 | 2008-08-13 | 深圳市赛茵斯爱速医疗科技有限公司 | Sample collecting device |
| CN101266243A (en) * | 2008-01-31 | 2008-09-17 | 程柯 | Instrument for collecting and rapidly detecting oral liquid |
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| CN102539752A (en) | 2012-07-04 |
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