CN1407340A - Reagent box for simultaneous assays of four diseases of teras and preparation thereof - Google Patents
Reagent box for simultaneous assays of four diseases of teras and preparation thereof Download PDFInfo
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- CN1407340A CN1407340A CN 01126586 CN01126586A CN1407340A CN 1407340 A CN1407340 A CN 1407340A CN 01126586 CN01126586 CN 01126586 CN 01126586 A CN01126586 A CN 01126586A CN 1407340 A CN1407340 A CN 1407340A
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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Abstract
This invention relates to a reagent box and a method to determine four diseases of ToRCH simultaneously. The reagent box is composed of immune osmotic gold marking combined determineation reactor, buffer solution and aurosol marking mixture. On the cellulose nitrate membrane in the said reactor, HCMV gene engineering antigen, HSV gene, engineering antigen, RV gene engineering antigen and TOX gene engineering antigen are spotted and also mass control substance on it. Meanwhile, preparation of the reagent box is disclosed. Four diseases are simultaneously determined, easily operated, quick and accurate.
Description
Technical field
The present invention relates to biological technical field, be specifically related to a kind of kit of detecting simultaneously four kinds of teratogenesis tire diseases and preparation method thereof that is used for.
Background technology
Economic development in recent years is swift and violent, and living standards of the people improve year by year, adds that domestic family planning work progressively moves towards in-depth, and only-child's ratio is higher, and people healthyly more and more pay attention to follow-on.How to improve neonate's quality, improve human inheritance's quality, i.e. it is extremely important that prenatal and postnatal care has seemed.Having avoided the individual birth of serious genetic disease and congenital disorders is the basic contents of prenatal and postnatal care.From the clinical eugenetics angle analysis, concrete work is included in mother's pregnancy duration mother and fetus is carried out the science of heredity inspection, gets rid of the birth of common genetic disease individuality as far as possible; And check at pregnancy duration whether mother suffers from the communicable disease that some easily causes fetal anomaly.
ToRCH is that the combination of four kinds of human disease microorganism English name prefixs comprises: cytomegalovirus (HCMV), herpes simplex virus (HSV), rubella virus (RV) and arc worm (TOX).They are pathogen that four kinds of most probables cause the infectious disease of monster, and the pregnant woman just can cause fetal anomaly as suffering from a kind of in these four kinds of infectious diseases, therefore, the inspection of these four kinds of pathogen has been become the essential items for inspection of pregnant woman's physical examination.
Cytomegalovirus belongs to the herpesviral group, and this virus is prevalent in the human body, and its infection sources is patient and asymptomatic subclinical infection person.In early days to the later stage, the pregnant woman can be by this virus infections from pregnancy.After infected, clinical symptoms is not obvious, or slight similar upper respiratory tract infection symptoms is arranged, as the discomfort of having a fever, fash, enlargement of lymph nodes etc.In general, early infection easily causes miscarriage, stillborn foetus, and the later stage infection mainly causes microcephalus, intelligence development obstacle, also has report can cause congenital heart disease, umbilical hernia, foot deformity and hepatosplenomegaly etc.
Herpes simplex is by the communicable disease due to the herpes simplex virus.Human HSV is the DNA viroid, is divided into amphitypy according to the difference of its former character: i.e. HSV-I and HSV-II.The people is the unique natural reservoir (of bird flu viruses) of simple virus, and virus is through in oral cavity, respiratory tract, genitals and the skin injury place intrusive body, and diving occupy in human body mucous membrane, blood, saliva, nerve fiber and the most organ.If the pregnant woman infects herpes simplex, gestation can cause nervous system malformations such as stillborn foetus, miscarriage, microcephalus, microphthalmia, the interior calcification of brain in early days, and its symptom is similar to cytomegalovirus.
Rubella virus is the strongest teratogenic factor of infectiousness, also is the most tangible a kind of virus of teratogenesis.The pregnant woman can have after by rubella virus infection rubella symptom or symptom smaller, therefore often easily is left in the basket.Infect more early, and it is high more, serious more that abnormal rate takes place fetus.Rubella virus is brought out congenital abnormality except that cataract, also has heart malformations (patent ductus arteriosus, atrium and interventricular septal defect), deafness, glaucoma, ommatidium, microcephaly, mental retardation and enamel damaged etc.The recent findings rubella virus can also cause growth retardation of fetus, cardiac damage.Suffering from rubella generation congenital abnormality can reach more than 40%.
Arc worm is narrow spectrum cytozoon, and it destroys human body all karyocytes except that red blood cell.Secondly arc worm main parasitic is eyes and heart at people's brain, so that morbid state and the alternately appearance of parasitic attitude, very big to the mankind's threat.Pregnant woman's toxoplasma gondii infection also can pass through the placental infection fetus, causes premature labor, stillbirth or fetal anomaly or other congenital disorders, especially causes feeblemindedness, has serious consequences.In China, annual more than 100 ten thousand deformed child and the congenital disabled youngsters relevant that are born with arch insect infection.
This shows, require the pregnant woman to do the detection of above four kinds of pathogen, be significant for prenatal and postnatal care.
At present, for the diagnosis of ToRCH, mostly adopt enzyme linked immunosorbent assay (ELISA) or immunofluorescence technique (IFA) to carry out.The reagent that this method relates to is more, complicated operation, and the reaction time is long.
The colloidal gold immunity percolation method is a technology that grows up on the basis of ELISA adsorption analysis method (ELISA), it is carrier with the miillpore filter, utilize the filterability and the capillarity of miillpore filter, make antigen and antibody reaction, the washing on a special percolating device, the mode of oozing filtration membrane with liquid is finished rapidly.
Because the method is simple, fast, except that reagent, do not need any instrument and equipment, a few minutes result that can detect by an unaided eye, in clinical detection, obtained at present widely and used.The colloidal gold immunity percolation method can be applicable to nearly all aspect of immunology detection, detect non-existent material (as the antibody of pathogen) in the normal body fluid but be mainly used in, and the extremely low and in particular cases unusual material that raises of normal contents is (as β-hCG).Because the improvement of the selected and technology of preparing of reagent raw material, range of application is more wide in recent years.But when having the people that the colloidal gold immunity percolation technology is used for multiple disease association antigen and antibody, there be limited evidence currently of detects.
Summary of the invention
Technical matters to be solved by this invention is to utilize the colloidal gold immunity percolation principle simultaneously the disease ToRCH of four kinds of teratogenesis tires to be detected, provide a kind of sensitivity, accurately, the kit of pregnant woman's blood examination rapidly.
The kit that is used for detecting simultaneously four kinds of teratogenesis tire diseases disclosed by the invention is made up of immunity percolation gold mark method joint inspection reaction unit, damping fluid, colloid gold label liquid, wherein on the nitrocellulose filter in immunity percolation gold mark method joint inspection reaction unit, put respectively at a distance of certain distance: the HCMV gene engineering antigen, the HSV gene engineering antigen, the RV gene engineering antigen, TOX gene engineering antigen and Quality Control thing sheep anti mouse IgM antibody, the point sample volume of antigen is 0.3-3 μ 1.
Colloid gold label liquid of the present invention is the golden label solution of mouse-anti people IgM monoclonal antibody, and mouse-anti people IgM monoclonal antibody is 80-120 μ g/ml by the protein concentration of colloid gold label, and wherein used colloid gold particle size is 20-60nm.
Damping fluid of the present invention is to contain the 0.01-0.05M that volumetric concentration is 0.01-0.07%Tween20, the PBS of PH7.2-8.8 (by potassium dihydrogen phosphate or sodium dihydrogen phosphate and the formulated damping fluid of sodium hydrogen phosphate) damping fluid.
The potpourri of the antigen that HSV gene engineering antigen of the present invention can be the HSV-I type, the antigen of HSV-II type or HSV-I type antigen and HSV-II type antigen.
Another technical matters to be solved by this invention provides the preparation method of above-mentioned detection kit.
The preparation method who is used for detecting simultaneously the kit of four kinds of teratogenesis tire diseases disclosed by the invention comprises the following steps: one, the preparation of application of sample thing on preparation immunity percolation gold mark method joint inspection reaction unit 1. films
The HCMV gene engineering antigen is placed 0.01M-0.05M, in the PBS solution of pH7.2-8.8,4 ℃ of dialysed overnight.HCMV gene engineering antigen after the dialysis uses the PBS solution dilution of pH7.2-8.8 to 0.2-2.0mg/ml, and 4 ℃ of preservations are standby.
The HSV gene engineering antigen is placed 0.01M-0.05M, in the PBS solution of pH7.2-8.8,4 ℃ of dialysed overnight.HSV gene engineering antigen after the dialysis uses the PBS solution dilution of pH7.2-8.8 to 0.2-2.0mg/ml, and 4 ℃ of preservations are standby.
The RV gene engineering antigen is placed 0.01M-0.05M, in the PBS solution of pH7.2-8.8,4 ℃ of dialysed overnight.RV gene engineering antigen after the dialysis uses the PBS solution dilution of pH7.2-8.8 to 0.2-2.0mg/ml, and 4 ℃ of preservations are standby.
The TOX gene engineering antigen is placed 0.01M-0.05M, in the PBS solution of pH7.2-8.8,4 ℃ of dialysed overnight.TOX gene engineering antigen after the dialysis uses the PBS solution dilution of pH7.2-8.8 to 0.2-2.0mg/ml, and 4 ℃ of preservations are standby.
Sheep anti mouse IgM antibody is placed 0.01M-0.05M, in the PBS solution of PH7.2-8.8,4 ℃ of dialysed overnight.Sheep anti mouse IgM antibody 0.01M-0.05M after the dialysis, the PBS solution dilution of pH7.2-8.8 are to 0.2-2.0mg/ml, and 4 ℃ of preservations are standby.2. application of sample on film
Draw above-mentioned HCMV gene engineering antigen, HSV gene engineering antigen, RV gene engineering antigen, TOX gene engineering antigen respectively, careful place is on the specific site of nitrocellulose filter, and the point sample volume is a 0.3-3 μ l/ point.
Other draws sheep anti mouse IgM antibody, makes a mark away from the place of premises sample position on same nitrocellulose filter, and this mark can be a bit, a line or other symbols.3. the aftertreatment of film
Above-mentioned nitrocellulose filter was put in 37 ℃ of baking ovens 30 minutes.Taking out the back room temperature places more than 20 minutes.Then it was immersed in 37 ℃ the confining liquid 20 minutes.After the taking-up, at room temperature place cleansing solution vibration washing 5-10 minute.Change cleansing solution, repeat above-mentioned vibration washing process.After the taking-up, dry under the room temperature.Preserve standby in 4 ℃ of dry environments.
Wherein said confining liquid is to contain the 0.01M-0.05M PBS damping fluid that volumetric concentration is the pH7.2-8.8 of 0.01-0.07%Tween20; Wherein said cleansing solution is to contain the 0.01M-0.05M PBS damping fluid that volumetric concentration is the pH7.2-8.8 of 0.01-0.07%Tween20.4. Zhuan Zhi assembling
Get two-layer absorbent filter pad in above-mentioned nitrocellulose filter below, again filter paper and film are packed into simultaneously and be fixed in the plastics reaction unit box.Two, preparation colloid gold label thing mixed liquor 1. preparation colloid gold particles
Get 0.01% aqueous solution of chloraurate, heated and boiled adds 1% citric acid, three sodium solutions fast, continues to boil 5 minutes, makes that the size of collaurum is 20-60nm.2. prepare colloid gold label liquid
Get the colloidal gold solution that grain size is 20-60nm, slowly add mouse-anti people IgM monoclonal antibody under magnetic agitation, making its ultimate density is 20-80 μ g/ml.Stirred 30 minutes under the room temperature.It is 0.2-1.0% that adding 10%BSA solution makes its final concentration, stirs 5 minutes under the room temperature.It is 0.1-0.5% that adding 10%PEG20000 solution makes its final concentration, stirs 5 minutes under the room temperature.Centrifugal 60 minutes of 12000-15000r/min carefully draws supernatant, discards.Precipitation is dissolved in preserves in the liquid, and with 0.45 μ m membrane filtration, it is standby to put 4 ℃ of preservations.Three, the preparation of damping fluid
Add Tween20 in the 0.01M-0.05M of pH7.2-8.8 PBS, the concentration that makes Tween20 is 0.01-0.07% (v/v).
Another technical matters to be solved by this invention is to disclose above-mentioned detection kit to detect application in cytomegalovirus, herpes simplex virus, the communicable disease that rubella virus and arc worm infected at the same time.
The detection method of kit of the present invention is as follows:
1. take out immunity percolation gold mark method joint inspection reaction unit, level places desktop;
2. in immunity percolation gold mark method joint inspection reaction unit hole, add two damping fluids, wetted surface;
3. add test serum 50ul, wait its diafiltration to go in the film;
4. add three damping fluids, treat that it infiltrates the back and adds two colloid gold label liquid;
5. add three damping fluids;
Have or not punctation 6.1 observe the relevant position after minute, have and think that then the disease virus of this position correspondence is positive in vivo, do not think then that the disease virus of this position correspondence is negative in vivo.
If HCMV antibody is arranged in the test serum, HSV antibody, a kind of in RV antibody and the TOX antibody or several, when nitrocellulose filter contacts with serum, antigen on the film can react with the corresponding antibody in the serum, and then reacts with the colloid gold label thing, thus colour developing.So, from situation which kind of virus of can having judged patient infection to be measured of colour developing.Because sheep anti mouse IgM antibody can not react with the antibody in the human serum, and the mouse-anti people IgM that contains in colloid gold label liquid can react with sheep anti mouse IgM antibody, will develop the color corresponding to the position of sheep anti mouse IgM antibody, is identified by naked eyes; When kit can't carry out test experience owing to the quality problems of certain component, just can not develop the color in the position corresponding to sheep anti mouse IgM antibody on the film, so, can carry out quality control to kit by sheep anti mouse IgM antibody.
Detection kit of the present invention has been utilized the principle of colloidal gold immunity percolation, make related antigen, the antibody of multiple virus disease on same percolating device (nitrocellulose filter), react, wash, by the colour developing situation of each spot of visual inspection, multiple teratogenesis tire virus is detected.
Kit of the present invention is compared with the ELISA kit, and its advantage is:
(1) detects many diseases
In same device, can carry out the detection of four kinds of communicable diseases.
(2) detect a plurality of antigens simultaneously
In same device, can detect a plurality of antigens, realize the integrated of multiple protein testing conditions.
(3) reaction time is short
Whole experiment can be finished in 3-5 minute, was fit to large-scale blood examination fast.This kit had only for 2 steps detected step, and promptly application of sample, reaction are all finished by diafiltration, detect overall process and only need several minutes, but susceptibility is similar with the ELISA experiment of finishing in need 1-2 hour.
(4) blood sampling volume is little
The serum amount that needs is 50ul only, only gathers to refer to that blood, ear blood get final product.And the ELISA method is surveyed an index and is just needed 100ul serum, and then testing four indices needs serum about 400ul.So this kit is very convenient for children, neonatal use, and the sampling of ELISA method can only be adopted vein, and is very painful to the neonate.
(5) testing result is not subjected to the influence of instrument; Be not subjected to the influence of reaction environment.
(6) adding the colloid gold label thing can develop the color, and operation steps is simple, and reagent can be in the room temperature long preservation.
Kit of the present invention utilizes the principle of colloidal gold immunity percolation method, on identical carrier, realize detecting in multiple antigen and the antibody, easy and simple to handle, rapid, accurate, detect applicable to how sick kind of various blood samples, for the detection of communicable disease provides new approaches.
Description of drawings
Point sample synoptic diagram on Fig. 1 nitrocellulose filter;
Wherein 1,2,3,4 represent the point sample position respectively.It between " C " 2 sheep anti-mouse igg antibody point sample position.
No. 1 serum of Fig. 2 detects and shows synoptic diagram, and testing result is all negative;
No. 2 serum of Fig. 3 detect and show synoptic diagram, and testing result shows is examined the people by arch insect infection;
No. 3 serum of Fig. 4 detect and show synoptic diagram, and testing result shows is examined the people by the CMV virus infections.
No. 4 serum of Fig. 5 detect and show that synoptic diagram, testing result show that being examined the people is infected simultaneously by CMV virus and HSV virus
It is positive that C-C represents the horizontal line of sheep anti mouse IgM antibody among Fig. 2-5.
EmbodimentOne, kit is selected antibody and material for use
Nitrocellulose filter is by S﹠amp; S company provides; Tested serum is provided by Shanghai City Center for Disease Control.Two, the preparation method of immunity percolation gold mark method joint inspection reaction unit is as follows:
(1) the HCMV gene engineering antigen is placed 0.02M, in the PBS solution of pH8.0,4 ℃ of dialysed overnight.HCMV gene engineering antigen after the dialysis uses the PBS solution dilution of pH8.0 to 1.0mg/ml, and 4 ℃ of preservations are standby.
(2) the HSV gene engineering antigen is placed 0.02M, in the PBS solution of pH8.0,4 ℃ of dialysed overnight.HSV gene engineering antigen after the dialysis uses the PBS solution dilution of pH7.2-8.8 to 1.0mg/ml, and 4 ℃ of preservations are standby.
(3) the RV gene engineering antigen is placed 0.02M, in the PBS solution of pH8.0,4 ℃ of dialysed overnight.RV gene engineering antigen after the dialysis uses the PBS solution dilution of pH7.2-8.8 to 1.0mg/ml, and 4 ℃ of preservations are standby.
(4) the TOX gene engineering antigen is placed 0.02M, in the PBS solution of pH8.0,4 ℃ of dialysed overnight.TOX gene engineering antigen after the dialysis uses the PBS solution dilution of pH7.2-8.8 to 1.0mg/ml, and 4 ℃ of preservations are standby.
(5) sheep anti mouse IgM antibody is placed 0.02M, in the PBS solution of pH8.0,4 ℃ of dialysed overnight.Sheep anti mouse IgM antibody 0.02M after the dialysis, the PBS solution dilution of pH8.0 are to 1.0mg/ml, and 4 ℃ of preservations are standby.
(6) as shown in Figure 1,1,2,3,4 positions on nitrocellulose filter, put last four kinds of albumen with micro sample adding appliance respectively: HCMV gene engineering antigen, HSV gene engineering antigen, RV gene engineering antigen, TOX gene engineering antigen, every point sample volume are 1.0 μ l; Be marked with at nitrocellulose filter between " C " 2, draw sheep anti mouse IgM antibody with the 0.2mm ruling pen and draw a horizontal line.
(7) this nitrocellulose filter is placed in 37 ℃ of baking ovens 30 minutes, take out the back room temperature and placed 25 minutes.Then it was immersed in 37 ℃ the confining liquid 20 minutes.After the taking-up, at room temperature place cleansing solution vibration washing 5 minutes, repeated washing for several times.Room temperature is dried.
(8) get two-layer absorbent filter pad in above-mentioned nitrocellulose filter below, again filter paper and film are packed into simultaneously and be fixed in the plastics reaction unit box.Three, damping fluid is to be 7.8 by pH, the solution that the PBS of 0.01M and 0.03% (v/v) Tween20 equal-volume mixes.Four, the preparation method of colloid gold label liquid
The grain size of the used collaurum of colloid gold label thing is 30nm.The preparation method of colloid gold label thing is as follows: get the colloidal gold solution that grain size is 30nm, slowly add mouse-anti people IgM monoclonal antibody under magnetic agitation, making its ultimate density is 30 μ g/ml.Stirred 30 minutes under the room temperature.It is 0.5% that adding 10%BSA solution makes its final concentration, stirs 5 minutes under the room temperature.It is 0.3% that adding 10%PEG20000 solution makes its final concentration, stirs 5 minutes under the room temperature.Centrifugal 60 minutes of 12000-15000r/min carefully draws supernatant, discards.Precipitation is dissolved in preserves in the liquid, and with 0.45 μ m membrane filtration, it is standby to put 4 ℃ of preservations.
Test different serum with this kit, data that hospital provides shows, the supplier of No. 1 serum does not suffer from and states in four kinds of viral diseases any; The supplier of No. 2 serum is bent capable insect infection; The supplier of No. 3 serum is by cytomegalovirus infection; The supplier of No. 4 serum is by cytomegalovirus and herpes simplex infection.Testing result is seen accompanying drawing 2, accompanying drawing 3, accompanying drawing 4, accompanying drawing 5 successively.
Claims (6)
1, a kind of kit that is used for detecting simultaneously four kinds of teratogenesis tire disease ToRCH, this kit is made up of immunity percolation gold mark method joint inspection reaction unit, damping fluid and colloid gold label liquid, it is characterized in that:
(1) on the nitrocellulose filter in the wherein said immunity percolation gold mark method joint inspection reaction unit, is shaped on HCMV gene engineering antigen, HSV gene engineering antigen, RV gene engineering antigen and four kinds of albumen of TOX gene engineering antigen and Quality Control thing sheep anti mouse IgM antibody at a distance of the certain distance point;
(2) wherein said colloid gold label liquid is the colloid gold label thing of mouse-anti people IgM monoclonal antibody.
2, a kind of kit as claimed in claim 1 is characterized in that mouse-anti people IgM monoclonal antibody is 80-120 μ g/ml by the protein concentration of colloid gold label in the wherein said colloid gold label liquid, and wherein used colloid gold particle size is 20-60nm.
3, a kind of kit as claimed in claim 1 or 2 is characterized in that wherein said HSV gene engineering antigen can be the antigen of the antigen of HSV-I type, HSV-II type or the potpourri of HSV-I type antigen and HSV-II type antigen
4, a kind of kit as claimed in claim 1 is characterized in that wherein said damping fluid is to contain the PBS damping fluid that volumetric concentration is the PH7.2-8.8 of 0.01-0.07%Tween20.
5, a kind of preparation method of kit as claimed in claim 1 is characterized in that the preparation method of kit comprises the following steps:
(1) preparation of immunity percolation gold mark method joint inspection reaction unit
(1) preparation of application of sample thing on the film
HCMV gene engineering antigen, HSV gene engineering antigen, RV gene engineering antigen and TOX gene engineering antigen are placed 0.01M-0.05M respectively, the PBS solution of pH7.2-8.8,4 ℃ of dialysed overnight.Each albumen after the dialysis uses the PBS solution dilution of pH7.2-8.8 to 0.2-2.0mg/ml respectively;
(2) application of sample on film
Draw above-mentioned HCMV gene engineering antigen, HSV gene engineering antigen, RV gene engineering antigen and TOX gene engineering antigen respectively, careful place on the specific site of nitrocellulose filter, every some 0.3-3 μ l; Other draws sheep anti mouse IgM antibody, makes a mark away from the place of premises sample position on same nitrocellulose filter, and this mark can be a bit, a line or other symbols;
(3) aftertreatment of film
This nitrocellulose filter was placed in 37 ℃ of baking ovens 30 minutes, take out the back room temperature and place more than 20 minutes, then it was immersed in 37 ℃ the confining liquid 20 minutes, after the taking-up, at room temperature place cleansing solution vibration washing 5-10 minute, the repeated washing several, room temperature is dried;
(4) assembling of reaction unit
Get two-layer absorbent filter pad in above-mentioned nitrocellulose filter below, again filter paper and film are packed into simultaneously and be fixed in the plastics reaction unit box;
(2) preparation colloid gold label thing mixed liquor
(1) preparation colloid gold particle
Get 0.01% aqueous solution of chloraurate, heated and boiled adds 1% citric acid, three sodium solutions fast, continues to boil 5 minutes, makes that the size of collaurum is 20-60nm;
(2) preparation of mouse-anti people IgM colloid gold label thing
Get the colloidal gold solution that grain size is 20-60nm, under magnetic agitation, slowly add mouse-anti people IgM monoclonal antibody, making its ultimate density is 20-80 μ g/ml, stirred 30 minutes under the room temperature, it is 0.2-1.0% that adding 10%BSA solution makes its final concentration, stirred 5 minutes under the room temperature, adding 10% PEG20000 solution, to make its final concentration be 0.1-0.5%, stirred 5 minutes under the room temperature, centrifugal 60 minutes of 12000-15000r/min carefully draws supernatant, discard, precipitation is dissolved in preserves in the liquid, and with 0.45 μ m membrane filtration, it is standby to put 4 ℃ of preservations;
(3) preparation of damping fluid
Add Tween20 in the PBS of pH7.2-8.8, the volumetric concentration that makes Tween20 is 0.01-0.07%.
6, a kind of kit as claimed in claim 1 detects the application in four kinds of teratogenesis tire diseases that cytomegalovirus, herpes simplex virus, rubella virus and arch insect infection cause at the same time.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01126586 CN1407340A (en) | 2001-08-29 | 2001-08-29 | Reagent box for simultaneous assays of four diseases of teras and preparation thereof |
| PCT/CN2001/001518 WO2003023403A1 (en) | 2001-08-29 | 2001-10-30 | A KIT FOR THE SIMULTANEOUS DIAGNOSIS OF FOUR KINDS OF DISEASES (ToRCH) RESULTING IN DEFORMITY FETUS AND A METHOD FOR PREPARATION OF IT |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01126586 CN1407340A (en) | 2001-08-29 | 2001-08-29 | Reagent box for simultaneous assays of four diseases of teras and preparation thereof |
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| Publication Number | Publication Date |
|---|---|
| CN1407340A true CN1407340A (en) | 2003-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 01126586 Pending CN1407340A (en) | 2001-08-29 | 2001-08-29 | Reagent box for simultaneous assays of four diseases of teras and preparation thereof |
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| CN (1) | CN1407340A (en) |
| WO (1) | WO2003023403A1 (en) |
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| CN101893629A (en) * | 2010-07-13 | 2010-11-24 | 南京谦和有汇生物科技有限公司 | TORCH screening test strip and preparation method thereof |
| CN108285932A (en) * | 2018-04-04 | 2018-07-17 | 重庆高圣生物医药有限责任公司 | A kind of probe, genetic chip, kit and detection method for TORCH detections |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103808928A (en) * | 2014-02-12 | 2014-05-21 | 严银芳 | Method for preparing ToRCH-ELISA (Enzyme Linked Immunosorbent Assay Kit) diagnosis reagent |
| CN109116031A (en) * | 2018-07-25 | 2019-01-01 | 滴准生物科技（常州）有限公司 | A kind of detection kit can detect a variety of prenatal and postnatal care pathogen IgG antibodies |
| CN116500279A (en) * | 2018-08-06 | 2023-07-28 | 宙斯生命科技(常州)有限公司 | Detection kit for cytomegalovirus antibody IgM and application |
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|---|---|---|---|---|
| US5637508A (en) * | 1993-03-26 | 1997-06-10 | Geo-Centers, Inc. | Biomolecules bound to polymer or copolymer coated catalytic inorganic particles, immunoassays using the same and kits containing the same |
| CN1140258A (en) * | 1995-07-05 | 1997-01-15 | 中国人民解放军第四军医大学 | Immune colloid gold reagent and method for testing hepatitis B virus antibody |
| JP2000097942A (en) * | 1998-09-18 | 2000-04-07 | Matsushita Electric Ind Co Ltd | Kit for immunochromatography |
| CN1114105C (en) * | 1999-11-01 | 2003-07-09 | 扬州大学 | Enzymoimmune reagent kit |
| CN1307238A (en) * | 2000-02-02 | 2001-08-08 | 昆明广博科技有限公司 | Fast test paper strip for various common venereal diseases and its preparation |
-
2001
- 2001-08-29 CN CN 01126586 patent/CN1407340A/en active Pending
- 2001-10-30 WO PCT/CN2001/001518 patent/WO2003023403A1/en active Application Filing
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101893629A (en) * | 2010-07-13 | 2010-11-24 | 南京谦和有汇生物科技有限公司 | TORCH screening test strip and preparation method thereof |
| CN108285932A (en) * | 2018-04-04 | 2018-07-17 | 重庆高圣生物医药有限责任公司 | A kind of probe, genetic chip, kit and detection method for TORCH detections |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003023403A1 (en) | 2003-03-20 |
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