CN102524602B - Expanded feeds for experimental dog and preparation method - Google Patents
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Abstract
The provides expanded feeds for an experimental dog, which comprise expanded corn flours, corn flours, flours, wheat flours, soybean meal, corn protein powders, bran powders, fish meal, chicken meal, egg yolk powders, trace elements, calcium hydrophosphate, yeast, table salt, fish oil, multivitamin and soybean oil which are mixed together; the expanded feeds for the experimental dog adopts the formula manner of combining vegetable protein with animal protein and carbohydrate, the trace elements necessary for growing are also added in the expanded feeds according to the physiological characteristics of the experimental dog, and the adequate demand of the experimental dog on the nutrition can be satisfied; in addition, the growth rate of the experimental dog is higher compared with conventional feeds and various physiological indices can completely satisfy the experimental animal national standards by adopting the expanded feeds for the experimental dog.
Description
Technical field
The present invention relates to a kind of animal feed, relate in particular expanded pellet diet of kind of experimental dog and preparation method thereof.
Technical background
Animal used as test refers to through artificial feeding, and its microorganism of carrying, parasite are carried out control, and the clear and definite or source of genetic background clearly is used for the animal of scientific research, teaching, production, calibrating and other scientific experiments.Animal used as test is a kind of lived experimental subjects, is described as " reagent of living ", " instrument of living ", has embodied the significance of animal used as test in quality management.The factor that affects Quality of Experimental Animals is many-sided, such as heredity, environment, drinking-water, feed etc.The animal used as test feeding quality is and the closely-related essential condition of Quality of Experimental Animals, also is that the assurance zoopery is carried out with experimental result basic accurately and reliably smoothly.Developed country reaches safely harm to the animal used as test feed hygiene and attaches great importance to, and China is along with the enforcement of GLP, also it is more and more paid close attention to and payes attention to.The standardization of animal used as test feed is the important component part of Laboratory Animal Standardization, is the prerequisite that improves Quality of Experimental Animals.
The Growth and reproduction of animal needs constantly to obtain nutrient and energy from feed.At the experimental animal feeding management aspect, not only to consider normal growth and the procreation of animal, more will consider between some animal individuals of experiment usefulness and reduce otherness as far as possible, must consider that also feed ingredient can not produce disturb experimental result, the phase obtains accurately reliably and experimental result repeatably.At present, to animal used as test, and animal impact that experimental result is produced not yet causes enough attention with disturbing by experiment about feed.In the allotment process, mixed feed tends to potential various unfavorable factor, might produce unexpected impact on the growth of animal used as test and on the reaction of experiment condition.
Summary of the invention
Research finds, the feed of experimental dog requires to contain relatively abundanter and the high protein of quality take the feed that comes from animal body as main, and concerning experimental dog, protein requires that not only amount is many, and amino acid requires more comprehensive, and is easy to digestion.The animal fodder that some must be arranged in the feed of experimental dog could satisfy beasle dog to the needs of protein.Animal fodder also contains abundant iron and b family vitamin.Simultaneously, added a large amount of carbohydrate in the expanded pellet diet of experimental dog provided by the invention.
In addition, the content of trace element in feed plays conclusive effect for the quality of the expanded pellet diet of experimental dog, these micro-content are crossed and low will be affected directly or indirectly that animal used as test grows normally, grows, the physiology course such as hereditary in the expanded pellet diet of experimental dog, and then affect result and the reliability of scientific experiment; Too high levels may cause again animal used as test to poison, and is light then affect growing of animal used as test, heavy then cause animal used as test death, or remains in the experimental animals, finally affects the scientific experiment result.So in the time of the conventional nutritional need of the expanded pellet diet of experimental dog of the present invention, also added the dog necessary trace element of growing.
For addressing the above problem, the invention provides a kind of expanded pellet diet of experimental dog, comprise the mixture that expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil, compound dimension he, soybean oil form in the test portion.
Described expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil, compound dimension he, the weight ratio of soybean oil are 5～15:5～15:10～20:5～10:10～20:5～15:5～15:2～10:1～5:3～12:1～5:1～8:1～8:1～3:1～3:0.1～2:2～7; Wherein, also be added with in every 100g mixture and add essence 1～3mL.
Described expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil, compound dimension he, the weight ratio of soybean oil are 10:10:15:6:12:10:9:5:1:5:1:2.8:3:1:1:0.2:4; Wherein, add essence 1mL in every 100g mixture.
Described trace element is the composition of iron, zinc, copper, molybdenum, selenium, iodine, manganese, cobalt element, wherein, the weight ratio of iron, zinc, copper, molybdenum, selenium, iodine, manganese, cobalt is 20～80:10～50:1～5:1～4:1～2:0.3～0.6:0.2～0.5:0.02～0.1.
He is the composition of vitamin A vitamin D, vitamin C, methionine, tryptophan, arginine, histidine, phenylalanine, tyrosine, threonine, leucine, isoleucine, valine for described compound dimension, wherein, the weight ratio of methionine, tryptophan, arginine, histidine, phenylalanine, tyrosine, threonine, leucine, isoleucine, valine is 1. 08～1.54:0.64～0.89:1.50～1.70:0.50～0.60:1.20～1.30:0.60～0.90:0.90～1.00:1.90～2.3:0.20～1.40:1.00～1.30; Addition in the expanded pellet diet of vitamin A, vitamin D, the described experimental dog of vitamin C is respectively 0.8～1.0 IU/kg, 9000～13500 IU/kg, 2000～4500 IU/kg.
A kind of preparation method of expanded pellet diet of experimental dog may further comprise the steps:
1) by the described prescription of one of above-mentioned prescription expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, milk powder, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt and liver oil pulverizing, uniform stirring are become mixed powder;
2) the mixed powder elevator rolling feeding of packing into, steam regulation of texture, conditioning of texture, the successively extruding of twin-screw swelling granular machine, oven dry, cooling obtains particulate material;
3) by go the described prescription of one of requirement 2-4 with the mode of oil spout evenly add compound dimension he, soybean oil and essence in particulate material, obtain the expanded pellet diet of experimental dog.
Material moisture is 17%～32% behind the described steam regulation of texture, conditioning of texture, and material temperature is 92 ℃ behind the steam regulation of texture, conditioning of texture; The screw speed 150r/min of extruding; 125 ℃ of kneading district machine barrel stage casing temperature; 40 ℃ of slaking district discharging section barrel zone temperatures.
Useful technique effect of the present invention is: the expanded pellet diet of experimental dog provided by the invention, the prescription mode that adopts vegetable protein, animal protein and carbohydrate to combine, and expanded pellet diet also added the necessary trace element of growing according to the physilogical characteristics of experimental dog, can satisfy experimental dog to the full requirements of nutrition; And adopting the expanded pellet diet of experimental dog provided by the invention, the experimental dog speed of growth is faster than conventional feed, and every physical signs all satisfies the animal used as test national standard fully.
The specific embodiment
The preparation 1 of the expanded pellet diet of embodiment 1 experimental dog
Be that the prescription of 10:10:15:6:12:10:9:5:1:5:1:2.8:3:1:1:0.2:4 takes by weighing by expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil, compound dimension he, the weight ratio of soybean oil, add essence 1mL in every 100g mixture.
Wherein, described trace element is the composition of iron, zinc, copper, molybdenum, selenium, iodine, manganese, cobalt element, wherein, the weight ratio of iron, zinc, copper, molybdenum, selenium, iodine, manganese, cobalt is 20～80:10～50:1～5:1～4:1～2:0.3～0.6:0.2～0.5:0.02～0.1.He is the composition of vitamin A vitamin D, vitamin C, methionine, tryptophan, arginine, histidine, phenylalanine, tyrosine, threonine, leucine, isoleucine, valine for described compound dimension, wherein, the weight ratio of methionine, tryptophan, arginine, histidine, phenylalanine, tyrosine, threonine, leucine, isoleucine, valine is 1. 08～1.54:0.64～0.89:1.50～1.70:0.50～0.60:1.20～1.30:0.60～0.90:0.90～1.00:1.90～2.3:0.20～1.40:1.00～1.30; Addition in the expanded pellet diet of vitamin A, vitamin D, the described experimental dog of vitamin C is respectively 0.8～1.0 IU/kg, 9000～13500 IU/kg, 2000～4500 IU/kg.
With corn flour, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, the cod-liver oil mixed powder elevator rolling feeding of packing into, steam regulation of texture, conditioning of texture, the successively extruding of twin-screw swelling granular machine, oven dry, cooling obtains particulate material;
With the mode of oil spout evenly add compound dimension he, soybean oil and essence in particulate material, wherein, material moisture is 17%～32% behind the described steam regulation of texture, conditioning of texture, material temperature is 92 ℃ behind the steam regulation of texture, conditioning of texture; The screw speed 150r/min of extruding; 125 ℃ of kneading district machine barrel stage casing temperature; 40 ℃ of slaking district discharging section barrel zone temperatures.Obtain the expanded pellet diet as the experimental dog of group I.
The preparation 2 of the expanded pellet diet of embodiment 2 experimental dogs
Take by weighing expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil, compound dimension he, soybean oil, its weight ratio is 5:5:10:5:10:5:5:2:1:3:1:1:1:1:1:0.1:2; Wherein, also be added with in every 100g mixture and add essence 1～3mL.
Obtain expanded pellet diet as the experimental dog of experimental group II by upper embodiment 1 described method.
The preparation 3 of the expanded pellet diet of embodiment 3 experimental dogs
Take by weighing expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil, compound dimension he, soybean oil, its weight ratio is 15:15:20:10:20:15:15:10:5:12:5:8:8:3:3:2:7; Wherein, also be added with in every 100g mixture and add essence 1～3mL.
Obtain expanded pellet diet as the experimental dog of experimental group III by upper embodiment 1 described method.
The feed biochemical indicator of group I experimental dog of embodiment 4 is investigated
16 of regular grade experiment primiparity beasle dogs are divided into female 8, and male 8, totally two groups.Stainless steel list cage is raised, and every day, three complete feeds of feeding were freely drunk water.Feed lot is away from the urban district, and raising condition and animal quality meet national animal used as test regular grade standard.The animal used as test dog expanded pellet diet of the group I of feeding detects than the every biochemical indicator of individual dog, and biochemical indicator as shown in Table 1, 2 and 3.
Table 1 group I animal used as test dog expanded pellet diet is on the impact of beasle dog Growth and development index
Table 2 group I animal used as test dog expanded pellet diet is on the impact of beasle dog physical signs
Remarks: WBC-leucocyte, LYM-lymphocyte, MON-monocyte, the NEU-neutrophil cell, EOS-eosinophil, BAS-basophilic granulocyte, the ALY-atypical lymphocyte, the huge immature cell of LIC-, RBC-red blood cell, the HGB-hemoglobin, HCT-packed cell volume, MCV-MCVU, the average hemoglobin content of MCH-, MCHC-NCHC, RDW-RDW, the PLT-blood platelet, MPV-mean platelet volume, PDW-thrombocytocrit.
Table 3 group I animal used as test dog expanded pellet diet is on the impact of beasle dog biochemical indicator
Remarks: ALP-alkaline phosphatase, CHOL-T-CHOL, TBIL-total bilirubin, TP-total protein, ALB-albumin, UREA-urea nitrogen, CREA-creatinine, AST-aspartic transaminase, ALT-alanine aminotransferase, GLU-blood sugar.
The feed biochemical indicator of experimental group II experimental dog of embodiment 5 is investigated
According to embodiment 4 described methods, the animal used as test dog expanded pellet diet of the experimental group of feeding II detects than the every biochemical indicator of individual dog, and biochemical indicator is shown in table 4,5 and 6.
Table 4 experimental group II animal used as test dog expanded pellet diet is on the impact of beasle dog Growth and development index
Table 5 experimental group II animal used as test dog expanded pellet diet is on the impact of beasle dog physical signs
Remarks: WBC-leucocyte, LYM-lymphocyte, MON-monocyte, the NEU-neutrophil cell, EOS-eosinophil, BAS-basophilic granulocyte, the ALY-atypical lymphocyte, the huge immature cell of LIC-, RBC-red blood cell, the HGB-hemoglobin, HCT-packed cell volume, MCV-MCVU, the average hemoglobin content of MCH-, MCHC-NCHC, RDW-RDW, the PLT-blood platelet, MPV-mean platelet volume, PDW-thrombocytocrit.
Table 6 experimental group II animal used as test dog expanded pellet diet is on the impact of beasle dog biochemical indicator
Remarks: ALP-alkaline phosphatase, CHOL-T-CHOL, TBIL-total bilirubin, TP-total protein, ALB-albumin, UREA-urea nitrogen, CREA-creatinine, AST-aspartic transaminase, ALT-alanine aminotransferase, GLU-blood sugar.
The feed biochemical indicator of experimental group III experimental dog of embodiment 6 is investigated
According to embodiment 4 described methods, the animal used as test dog expanded pellet diet of the experimental group of feeding III detects than the every biochemical indicator of individual dog, and biochemical indicator is shown in table 7,8 and 9.
Table 7 experimental group III animal used as test dog expanded pellet diet is on the impact of beasle dog Growth and development index
Table 8 experimental group III animal used as test dog expanded pellet diet is on the impact of beasle dog physical signs
Remarks: WBC-leucocyte, LYM-lymphocyte, MON-monocyte, the NEU-neutrophil cell, EOS-eosinophil, BAS-basophilic granulocyte, the ALY-atypical lymphocyte, the huge immature cell of LIC-, RBC-red blood cell, the HGB-hemoglobin, HCT-packed cell volume, MCV-MCVU, the average hemoglobin content of MCH-, MCHC-NCHC, RDW-RDW, the PLT-blood platelet, MPV-mean platelet volume, PDW-thrombocytocrit.
Table 9 experimental group III animal used as test dog expanded pellet diet is on the impact of beasle dog biochemical indicator
Remarks: ALP-alkaline phosphatase, CHOL-T-CHOL, TBIL-total bilirubin, TP-total protein, ALB-albumin, UREA-urea nitrogen, CREA-creatinine, AST-aspartic transaminase, ALT-alanine aminotransferase, GLU-blood sugar.
Claims (6)
1. the expanded pellet diet of an experimental dog is characterized in that: feed is the mixture that expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil, compound dimension he, soybean oil form;
Described expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil, compound dimension he, the weight ratio of soybean oil are 5～15:5～15:10～20:5～10:10～20:5～15:5～15:2～10:1～5:3～12:1～5:1～8:1～8:1～3:1～3:0.1～2:2～7; Wherein, also be added with in every 100g mixture and add essence 1～3mL.
2. the expanded pellet diet of experimental dog according to claim 1 is characterized in that: described expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil, compound dimension he, the weight ratio of soybean oil are 10:10:15:6:12:10:9:5:1:5:1:2.8:3:1:1:0.2:4; Wherein, add essence 1mL in every 100g mixture.
3. the expanded pellet diet of experimental dog claimed in claim 2, it is characterized in that: described trace element is the composition of iron, zinc, copper, molybdenum, selenium, iodine, manganese, cobalt element, wherein, the weight ratio of iron, zinc, copper, molybdenum, selenium, iodine, manganese, cobalt is 20～80:10～50:1～5:1～4:1～2:0.3～0.6:0.2～0.5:0.02～0.1.
4. the expanded pellet diet of experimental dog claimed in claim 2, it is characterized in that: he is vitamin A described compound dimension, vitamin D, vitamin C, methionine, tryptophan, arginine, histidine, phenylalanine, tyrosine, threonine, leucine, isoleucine, the composition of valine, wherein, methionine, tryptophan, arginine, histidine, phenylalanine, tyrosine, threonine, leucine, isoleucine, the weight ratio of valine is 1.08～1.54:0.64～0.89:1.50～1.70:0.50～0.60:1.20～1.30:0.60～0.90:0.90～1.00:1.90～2.3:0.20～1.40:1.00～1.30; Addition in the expanded pellet diet of vitamin A, vitamin D, the described experimental dog of vitamin C is respectively 0.8～1.0 IU/kg, 9000～13500 IU/kg, 2000～4500 IU/kg.
5. the preparation method of the expanded pellet diet of an experimental dog is characterized in that: may further comprise the steps:
1) by the described prescription of one of claim 1-4 expanded corn powder, corn flour, flour, wheat flour, bean cake powder, corn protein powder, wheatfeed, fish meal, chicken meal, yolk powder, trace element, calcium monohydrogen phosphate, yeast, salt, cod-liver oil pulverizing, uniform stirring are become mixed powder;
2) the mixed powder elevator rolling feeding of packing into, steam regulation of texture, conditioning of texture, the successively extruding of twin-screw swelling granular machine, oven dry, cooling obtains particulate material;
3) by the described prescription of one of claim 1-4 with the mode of oil spout evenly add compound dimension he, soybean oil and essence in particulate material, obtain the expanded pellet diet of experimental dog.
6. the preparation method of the expanded pellet diet of experimental dog according to claim 5, it is characterized in that: material moisture is 17%～32% behind the described steam regulation of texture, conditioning of texture, material temperature is 92 ℃ behind the steam regulation of texture, conditioning of texture; The screw speed 150r/min of extruding; 125 ℃ of kneading district machine barrel stage casing temperature; 40 ℃ of slaking district discharging section barrel zone temperatures.
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| CN105454697A (en) * | 2015-12-21 | 2016-04-06 | 朱秀琴 | Pet dog food formula |
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| CN109287854A (en) * | 2018-09-27 | 2019-02-01 | 佛山市雷米高动物营养保健科技有限公司 | Improve composition, the product and preparation method thereof of female pet reproductive performance |
| CN109924364A (en) * | 2019-03-28 | 2019-06-25 | 江苏财经职业技术学院 | The dry type puff pet dog diet produced using crayfish head shrimp shell meal |
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| JP2636409B2 (en) * | 1989-03-24 | 1997-07-30 | 日清製粉株式会社 | Granular feed for young animals |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1605266A (en) * | 2004-11-12 | 2005-04-13 | 上海生物制品研究所 | Cleaning grade mixing fodder for golden hamster |
| KR20100009214A (en) * | 2008-07-18 | 2010-01-27 | 이선채 | The composition of musk rat feed, and the breeding method of musk rat by the above composition |
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