CN102524391B - Fermented dairy product containing compound prebiotic and probiotic group - Google Patents
Fermented dairy product containing compound prebiotic and probiotic group Download PDFInfo
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- CN102524391B CN102524391B CN2010105974153A CN201010597415A CN102524391B CN 102524391 B CN102524391 B CN 102524391B CN 2010105974153 A CN2010105974153 A CN 2010105974153A CN 201010597415 A CN201010597415 A CN 201010597415A CN 102524391 B CN102524391 B CN 102524391B
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Abstract
The invention discloses a fermented dairy product containing a compound prebiotic and probiotic group. Lactobacillus bulgaricus, Streptococcus thermophilus, Bifidobacterium lactis BB-12 and Lactobacillus acidophilus are added into the fermented dairy product. Two kinds of prebiotics, namely fructooligosaccharide and galactooligosacchride are also added into the fermented dairy product; and the content of the fructooligosaccharide is 0.25 to 60g and the content of the galactooligosacchride is 0.25 to 60g in 1,000g of fermented dairy product raw material, and the content sum of the fructooligosaccharide and the galactooligosacchride is 0.5 to 120g. By the fermented dairy product containing the compound prebiotic and probiotic group, the phenomenon that the excessive eating of a fermented dairy product may cause flatulence, exhaust and diarrhea of a small number of users when single prebiotics are used is avoided, and Bifidobacterium and lactobacillus in intestinal tracts have a good proliferation effect; and meanwhile, the fermented dairy product slightly proliferates enterococcus, enterobacter and Clostridium perfringens, and produces low pH values in the intestinal tracts.
Description
Technical field
The present invention relates to a kind of fermented dairy product that contains prebiotics and beneficial flora, relate in particular to a kind of fermented dairy product that contains complex prebiotics and beneficial flora.
Background technology
The Yoghourt that exists in the market is mainly by lactobacillus bulgaricus and streptococcus thermophilus fermentation, also added in addition various probios such as DN-173010(Bifidobacterium), LGG etc., probio is for promoting intestinal health and then promoting people's health effect to be rooted in the hearts of the people.
Thereby the growth that prebiotics refers to optionally to stimulate the bacterium in a kind of or minority kind bacterium colony with active and to the host produce wholesome effect improve host's health can not be digested food composition.Thereby prebiotics passes through the optionally interior beneficial bacterium of paraphyte produces health efficacy to human body.Prebiotics usually (is called symphysis unit) thereby adds performance intestinal health effect in product to together with probio.Also there is difference in each individuality to the tolerance of prebiotics, is used alone prebiotics, though its effect is good, if excessive amount may cause that a few peoples' flatulence, exhaust are even suffered from diarrhoea etc. sometimes.
Summary of the invention
The purpose of this invention is to provide a kind of fermented dairy product that contains complex prebiotics and beneficial flora, while avoiding using single prebiotics, excessive edible fermented dairy product may cause that flatulence, exhaust that a few peoples occur even suffer from diarrhoea.
Another object of the present invention is to provide a kind of fermented dairy product that contains complex prebiotics and beneficial flora, make in enteron aisle the cultivation effect of Bifidobacterium and Lactobacillus good, simultaneously, its propagation to enterococcus, enterobacteria and C.perfringens is few, and produces lower pH value in enteron aisle.
The invention provides a kind of fermented dairy product that contains complex prebiotics and beneficial flora, it is added with lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium lactis BB-12 and lactobacillus acidophilus, has also added two kinds of prebioticses of FOS and galactooligosaccharide in fermented dairy product; The content of stating FOS in 1000g fermented dairy product raw material is 0.25 to 60g, and the content of galactooligosaccharide is 0.25 to 60g, and the scope of FOS and Determination of galactooligosacchariin sum is 0.5 to 120g.
In another schematic embodiment of a kind of fermented dairy product that contains complex prebiotics and beneficial flora, the bifidobacterium lactis BB-12 content that adds is 10
5To 10
7CFU/g, the content of lactobacillus acidophilus are 10
5To 10
7CFU/g.
Also there is difference in each individuality to the tolerance of prebiotics, be used alone prebiotics, its effect amount is often larger, may cause a few peoples' the phenomenon such as generation diarrhoea, flatulence, and use complex prebiotics not only can reduce effect amount cost, and can avoid as far as possible above-mentioned phenomenon.
a kind of fermented dairy product that contains complex prebiotics and beneficial flora, by external fecal bacteria culture experiment, filter out complex prebiotics combination galactooligosaccharide (GOS) and FOS (FOS) with cooperative synergism effect, this combination is to the cultivation effect of beneficial bacterium Bifidobacterium and Bacillus acidi lactici good (12 hours countings), simultaneously, it is to enterococcus, the propagation of enterobacteria and C.perfringens few (12 hours countings), galactooligosaccharide (GOS) and FOS (FOS) combination results less pH value (12 hours counting), the reduction of pH value is for the health of keeping whole enteron aisle, reduce the growth of harmful bacteria and for the absorption of mineral matter, all play vital effect.
The specific embodiment
Embodiment 1
A kind of fermented dairy product that contains complex prebiotics and beneficial flora, its formula following (in the components by weight percent of 1000 gram raw materials):
White granulated sugar: 75 grams;
PURE WHEY: 2 grams;
FOS: 5 grams;
Galactooligosaccharide: 5 grams;
Food additives: 5 grams;
Lactobacillus bulgaricus adds streptococcus thermophilus: 0.02U;
Bifidobacterium lactis BB-12:10
5CFU/g;
Lactobacillus acidophilus: 10
5CFU/g;
All the other are supplied with clean newborn raw milk.
Below a kind of preparation technology who contains the fermented dairy product of complex prebiotics and beneficial flora:
1) raw milk is only newborn, obtains clean newborn raw milk;
2) batching: add in the clean newborn raw milk of circulation after food additives, white granulated sugar, lactalbumin, FOS, galactooligosaccharide are mixed, open and stir when batching starts, after batching is completed, stop circulation, directly carry out the push pipe operation;
3) use clean newborn raw milk constant volume, obtain feed liquid;
4) to the feed liquid preheating: 65 ℃ of preheat temperatures;
5) degassed to feed liquid: degassed temperature is 65 ℃, and pressure is-90Kpa;
6) to the feed liquid homogeneous: homogenization pressure is 180bar;
7) to the feed liquid sterilization: sterilization temperature is 95 ℃, and the time is 300 seconds;
8) feed liquid is cooling: be cooled to 42 ℃;
9) inoculation, fermentation: when cooled feed liquid is entered fermentation tank 1/3, start and stir, add in proportion bacterial classification, continue to stir 10 to 15 minutes after charging finishes; Stop stirring, start fermentation, fermentation temperature is 42 ℃;
10) fermentation termination is measured: fermentation termination is controlled at 75
0T, obtain acidified milk;
11) cooling filling: the acidified milk after acidity is touched the mark is cooled to 25 ℃, squeezes into the Yoghourt storage tank, and this acidified milk stirred 15 seconds after all entering storage tank, and jam is added in acidified milk.
Embodiment 2
A kind of fermented dairy product that contains complex prebiotics and beneficial flora, its formula following (in the components by weight percent of 1000 gram raw materials):
White granulated sugar: 70 grams;
PURE WHEY: 2 grams;
Lactobacillus bulgaricus adds streptococcus thermophilus: 0.3U;
Bifidobacterium lactis BB-12:10
7CFU/g;
Lactobacillus acidophilus: 10
7CFU/g;
FOS: 0.25 gram;
Galactooligosaccharide: 0.25 gram;
Food additives: 3 grams;
Strawberry fruit grain: 75 grams;
All the other are supplied with clean newborn raw milk.
Embodiment 3
A kind of fermented dairy product that contains complex prebiotics and beneficial flora, its formula following (in the components by weight percent of 1000 gram raw materials):
White granulated sugar: 70 grams;
PURE WHEY: 2 grams;
Lactobacillus bulgaricus adds streptococcus thermophilus: 0.2U;
Lactobacillus acidophilus: 10
6CFU/g;
Bifidobacterium lactis BB-12:10
6CFU/g;
FOS: 60 grams;
Galactooligosaccharide: 60 grams;
Food additives: 3 grams;
Haw berry grain: 30 grams;
Red jujube fruit grain: 30 grams;
All the other are supplied with clean newborn raw milk.
External fecal bacteria culture experiment
1. given the test agent: the stool suspension of taking from normal adults.
2. prebiotic preparation
Galactooligosaccharide (GOS): Determination of galactooligosacchariin 〉=42%, liquid state, Dutch Friesland;
Xylo-oligosaccharide: Xylose Content 〉=70%, Shandong dragon power;
Inulin: inulin content 〉=99.5%, average degree of polymerization 〉=23, Belgian ORAFTI;
FOS (FOS): FOS content 〉=41%, Guangxi Ao Ligao.
3. culture medium
The EMB culture medium, Sodium azide-crystal violet-aesculin agar medium, the LBS agar medium, the BBL agar medium, the TSC agar medium, all be purchased from Hai Bo Bioisystech Co., Ltd.
Sugar-free GAM culture medium: soya peptone 3g, peptone 10g, digestion serum powder 13.5g, yeast extract 5g, beef extract 2.2g, beef liver 1.2g, glucose 3g, KH
2PO
42.5g, soluble starch 5g, Cys salt 0.3g, sodium thioglycollate 0.3g, OX-heart soup 1000mL, transfer pH7.2.
4. laboratory apparatus
The SW-CJ superclean bench, BBL anaerobic jar, anaerobic culture box, DNP-9028 constant incubator, pressure steam sterilization boiler, pH211 precision acidity meter, biochemical intelligent incubator, thermostat water bath.
5. test method, test and press table 1 grouping.
Table 1 test grouping situation.
Test group | Medium component |
Contrast 1 | Sugar-free GAM culture medium |
Contrast 2 | GAM culture medium+1% glucose |
Experimental group 1 | GAM culture medium+1%GOS |
Experimental group 2 | GAM culture medium+1%XOS |
Experimental group 3 | GAM culture medium+1% inulin |
Experimental group 4 | GAM culture medium+1%FOS |
Experimental group 5 | GAM culture medium+1%GOS+FOS (1:1) |
Experimental group 6 | GAM culture medium+1%FOS+XOS (1:1) |
Experimental group 7 | The GAM culture medium+1%GOS+XOS(1:1) |
Experimental group 8 | GAM culture medium+1%FOS+ inulin (1:1) |
Establish 3 Duplicate Samples for every group.250 ml nutrient solutions divide and are filled to infusion bottle, jump a queue and plug syringe needle, extract immediately syringe needle after 115 ℃ of sterilizing 20 min are sterilizing.Get fresh excreta 1g and add in 200ml physiological saline fully and mix, get supernatant 1ml and add respectively and respectively organize in nutrient solution, 37 ℃ of cultivations.Detect after respectively organizing nutrient solution and cultivating the quantity of Bifidobacterium, Bacillus acidi lactici, enterobacteria, enterococcus and C.perfringens after 6h, 12h, measure simultaneously the pH of zymotic fluid.
6. method of counting
Get zymotic fluid 1ml, add fully dilution in physiological saline 9 ml, then get this liquid 1ml, add fully dilution in physiological saline 9ml, gradient dilution, select the suitable different intestinal microfloras of gradient counting, the operation of according to the form below method and counting.
The cultivation method of counting of table 2 enteron aisle flora.
The enteron aisle bacterium | Culture medium | Condition of culture | Counting |
Bifidobacterium | The BBL culture medium, add neomycinsulphate 100 μ g/ml, sodium propionate 15mg/ml, lithium chloride 3mg/ml | Anaerobism, 37 ℃, 48h | Counting white or milky bacterium colony, G +Irregular bacillus bacterium colony |
Lactobacillus | The LBS culture medium | Anaerobism, 37 ℃, 48h | White or milky bacterium colony, G +Bacillus |
Enterobacteria | The EMB culture medium | Aerobic, 37 ℃, 24h | Red bacterium colony, G —Bacillus |
Enterococcus | Sodium azide-crystal violet-aesculin agar medium | Aerobic, 37 ℃, 24h | Obvious brown haloing, G +Coccus |
C.perfringens | TSC culture medium+additive | Anaerobism, 37 ℃, 48h | The bacterium colony of black fluorescence under ultraviolet light |
7. results and analysis.
The cultivation counting (Log (cfu/mL)) of table 3 pair Bifidobacterium.
Prebiotics | 0 hour | 6 hours | 12 hours |
Contrast 1 | 1.60 | 1.85 | 1.60 |
Contrast 2 | 1.60 | 5.60 | 5.70 |
The FOS+ inulin | 1.60 | 5.00 | 6.26 |
FOS+GOS | 1.60 | 6.11 | 6.48 |
FOS+XOS | 1.60 | 5.08 | 6.28 |
FOS | 1.60 | 5.78 | 6.00 |
GOS+XOS | 1.60 | 5.58 | 5.00 |
GOS | 1.60 | 5.00 | 5.60 |
Inulin | 1.60 | 5.32 | 5.30 |
XOS | 1.60 | 5.34 | 5.00 |
The cultivation counting (Log (cfu/mL)) of table 4 pair Bacillus acidi lactici.
Prebiotics | 0 hour | 6 hours | 12 hours |
Contrast 1 | 4.38 | 4.60 | 4.47 |
Contrast 2 | 4.38 | 9.00 | 8.79 |
The FOS+ inulin | 4.38 | 8.85 | 7.41 |
FOS+GOS | 4.38 | 8.89 | 9.04 |
FOS+XOS | 4.38 | 8.64 | 8.88 |
FOS | 4.38 | 8.89 | 8.14 |
GOS+XOS | 4.38 | 8.82 | 8.56 |
GOS | 4.38 | 8.73 | 8.83 |
Inulin | 4.38 | 8.51 | 8.00 |
XOS | 4.38 | 8.48 | 8.49 |
The cultivation counting (Log (cfu/mL)) of table 5 pair enterobacteria.
Prebiotics | 0 hour | 6 hours | 12 hours |
Contrast 1 | 3.48 | 7.83 | 8.49 |
Contrast 2 | 3.48 | 8.18 | 8.87 |
The FOS+ inulin | 3.48 | 7.30 | 8.40 |
FOS+GOS | 3.48 | 7.90 | 8.15 |
FOS+XOS | 3.48 | 8.28 | 8.20 |
FOS | 3.48 | 7.97 | 8.32 |
GOS+XOS | 3.48 | 8.48 | 8.48 |
GOS | 3.48 | 8.16 | 8.26 |
Inulin | 3.48 | 6.00 | 8.60 |
XOS | 3.48 | 8.30 | 8.24 |
Table 6 pair enterococcal cultivation counting (Log (cfu/mL)).
Prebiotics | 0 hour | 6 hours | 12 hours |
Contrast 1 | 2.34 | 4.86 | 6.82 |
Contrast 2 | 2.34 | 5.70 | 7.05 |
The FOS+ inulin | 2.34 | 5.69 | 7.30 |
FOS+GOS | 2.34 | 5.80 | 6.45 |
FOS+XOS | 2.34 | 5.58 | 6.56 |
FOS | 2.34 | 5.73 | 7.08 |
GOS+XOS | 2.34 | 5.67 | 6.58 |
GOS | 2.34 | 5.81 | 6.52 |
Inulin | 2.34 | 4.80 | 7.45 |
XOS | 2.34 | 5.71 | 6.57 |
The cultivation counting (Log (cfu/mL)) of table 7 pair C.perfringens.
Prebiotics | 0 hour | 6 hours | 12 hours |
Contrast 1 | 2.70 | 3.85 | 7.40 |
Contrast 2 | 2.70 | 3.90 | 6.00 |
The FOS+ inulin | 2.70 | 3.88 | 6.85 |
FOS+GOS | 2.70 | 4.52 | 5.90 |
FOS+XOS | 2.70 | 4.36 | 6.20 |
FOS | 2.70 | 4.31 | 6.04 |
GOS+XOS | 2.70 | 4.58 | 6.30 |
GOS | 2.70 | 4.83 | 6.22 |
Inulin | 2.70 | 4.60 | 7.00 |
XOS | 2.70 | 5.00 | 6.60 |
The rear impact on the pH value of table 8 combination of prebiotics fermentation.
Prebiotics | 6 hours | 12 hours |
Contrast 1 | 6.78 | 6.43 |
Contrast 2 | 5.57 | 4.42 |
The FOS+ inulin | 6.59 | 4.87 |
FOS+GOS | 6.04 | 4.67 |
FOS+XOS | 6.31 | 4.65 |
FOS | 6.73 | 5.57 |
GOS+XOS | 6.01 | 4.73 |
GOS | 6.14 | 4.77 |
Inulin | 6.14 | 4.71 |
XOS | 7.06 | 6.25 |
Result shows, GOS and FOS combination are good to the cultivation effect of beneficial bacterium Bifidobacterium and Lactobacillus, simultaneously, propagation to enterobacteria, enterococcus and C.perfringens is few, GOS and FOS combination results lower pH value, the reduction of pH value, for the health of keeping whole enteron aisle, reduces the growth of harmful bacteria and for the absorption of mineral matter, all plays vital effect.Therefore, determine that GOS and FOS are optimum combination of prebiotics.The combination of GOS and FOS surpasses uses separately FOS and GOS, illustrates that there is synergistic function in the combination of FOS and GOS.Therefore, application GOS and FOS, can effectively solve the FOS that uses single high dose and the phenomenons such as the exhaust that brings, diarrhoea, reached the effect of higher single prebiotics with lower complex prebiotics amount, and reduced the effect cost.
It will be understood by those skilled in the art that for table 3 to the count value in table 7 and the pH value in table 8, mainly take 12 hours some data as reference.
In this article, " schematically " expression " is served as example, example or explanation ", any diagram, the embodiment that is described in this article " schematically " should be interpreted as a kind of preferred or have more the technical scheme of advantage.
Be to be understood that, although this specification is described according to each embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification is only for clarity sake, those skilled in the art should make specification as a whole, technical scheme in each embodiment also can, through appropriate combination, form other embodiments that it will be appreciated by those skilled in the art that.
Above listed a series of detailed description is only illustrating for feasibility embodiment of the present invention; they are not in order to limit the scope of the invention, all disengaging within equivalent embodiment that skill spirit of the present invention does or change all should be included in protection scope of the present invention.
Claims (2)
1. fermented dairy product that contains complex prebiotics and beneficial flora, it is added with lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium lactis BB-12 and lactobacillus acidophilus, has also added two kinds of prebioticses of FOS and galactooligosaccharide in described fermented dairy product; The content of FOS described in the described fermented dairy product raw material of every 1000g is 0.25 to 60g, the content of described galactooligosaccharide is 0.25 to 60g, and the scope of described FOS and described Determination of galactooligosacchariin sum is 0.5 to 120g, it is characterized in that, the content of described FOS and described galactooligosaccharide is than being 1:1.
2. fermented dairy product as claimed in claim 1, the described bifidobacterium lactis BB-12 content that wherein adds is 10
5To 10
7CFU/g, the content of lactobacillus acidophilus are 10
5To 10
7CFU/g.
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CN102845518A (en) * | 2012-10-17 | 2013-01-02 | 石家庄君乐宝乳业有限公司 | Yoghourt with health-care function, as well as preparation method and application thereof |
TWI554214B (en) * | 2013-02-08 | 2016-10-21 | 愛之味股份有限公司 | Process for preparing a functional fermented milk product with low acidity and containing a low content of lactose and enhanced with galactooligosaccharides and bifidobacterium, and functional fermented milk product prepared therewith |
CN104186655B (en) * | 2014-08-27 | 2016-06-29 | 内蒙古伊利实业集团股份有限公司 | There is low-fat yogurt of blood fat reducing and anti-oxidation function and preparation method thereof |
CN104719475A (en) * | 2015-02-11 | 2015-06-24 | 江门市德昂生物科技有限公司 | Composition for producing dairy products and production method of dairy products |
CN106819107A (en) * | 2016-12-29 | 2017-06-13 | 石家庄君乐宝乳业有限公司 | Leben with the effect of whole intestines and preparation method thereof |
CN112741334B (en) * | 2019-10-29 | 2023-03-21 | 内蒙古伊利实业集团股份有限公司 | Prebiotic compositions, and uses thereof |
CN111487332A (en) * | 2019-11-08 | 2020-08-04 | 天津科技大学 | Evaluation method and application of prebiotics effect of fructo-oligosaccharide with single polymerization degree |
CN110710571A (en) * | 2019-11-25 | 2020-01-21 | 上海昊岳食品科技有限公司 | Functional yoghourt and preparation method thereof |
CN111011866B (en) * | 2019-12-12 | 2023-03-24 | 上海理工大学 | Application of prebiotics in promoting growth of lactobacillus plantarum in intestinal environment |
CN112998276B (en) * | 2021-02-01 | 2023-08-29 | 天津科技大学 | Synbiotic compound preparation for preventing diarrhea, intestinal leakage and sepsis caused by excessive intake of prebiotics, preparation method and application |
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