CN102524391A - Fermented dairy product containing compound prebiotic and probiotic group - Google Patents

Fermented dairy product containing compound prebiotic and probiotic group Download PDF

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Publication number
CN102524391A
CN102524391A CN2010105974153A CN201010597415A CN102524391A CN 102524391 A CN102524391 A CN 102524391A CN 2010105974153 A CN2010105974153 A CN 2010105974153A CN 201010597415 A CN201010597415 A CN 201010597415A CN 102524391 A CN102524391 A CN 102524391A
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dairy product
fermented dairy
fos
prebiotics
content
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CN102524391B (en
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刘爱萍
石欢
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Inner Mongolia Mengniu Dairy Group Co Ltd
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Inner Mongolia Mengniu Dairy Group Co Ltd
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Abstract

The invention discloses a fermented dairy product containing a compound prebiotic and probiotic group. Lactobacillus bulgaricus, Streptococcus thermophilus, Bifidobacterium lactis BB-12 and Lactobacillus acidophilus are added into the fermented dairy product. Two kinds of prebiotics, namely fructooligosaccharide and galactooligosacchride are also added into the fermented dairy product; and the content of the fructooligosaccharide is 0.25 to 60g and the content of the galactooligosacchride is 0.25 to 60g in 1,000g of fermented dairy product raw material, and the content sum of the fructooligosaccharide and the galactooligosacchride is 0.5 to 120g. By the fermented dairy product containing the compound prebiotic and probiotic group, the phenomenon that the excessive eating of a fermented dairy product may cause flatulence, exhaust and diarrhea of a small number of users when single prebiotics are used is avoided, and Bifidobacterium and lactobacillus in intestinal tracts have a good proliferation effect; and meanwhile, the fermented dairy product slightly proliferates enterococcus, enterobacter and Clostridium perfringens, and produces low pH values in the intestinal tracts.

Description

A kind of fermented dairy product that contains complex prebiotics and beneficial flora
Technical field
The present invention relates to a kind of fermented dairy product that contains prebiotics and beneficial flora, relate in particular to a kind of fermented dairy product that contains complex prebiotics and beneficial flora.
Background technology
The sour milk that exists in the market mainly is by lactobacillus bulgaricus and streptococcus thermophilus fermentation; Also added various probios such as DN-173010 (Bifidobacterium), LGG etc. in addition, probio is for promoting intestinal health and then promoting people's health effect to be rooted in the hearts of the people.
Prebiotics is meant that thereby the growth that optionally stimulates the bacterium in a kind of or the minority kind bacterium colony produces wholesome effect with activity to the host and improves the healthy food composition that can not be digested of host.The interior beneficial bacterium of paraphyte produces health efficacy to human body thereby prebiotics passes through optionally.Prebiotics usually with probio (being called symphysis unit) thus add in the product performance intestinal health effect to.Also there is difference in each individuality to the tolerance of prebiotics, uses a kind of prebiotics separately, though its effect is good, if excessive amount possibly cause a few peoples' flatulence, exhaust even diarrhoea etc. sometimes.
Summary of the invention
The purpose of this invention is to provide a kind of fermented dairy product that contains complex prebiotics and beneficial flora, when avoiding the use of single prebiotics, excessive edible fermented dairy product possibly cause flatulence, exhaust even the diarrhoea that a few peoples occur.
Another object of the present invention provides a kind of fermented dairy product that contains complex prebiotics and beneficial flora; Make that the cultivation effect of Bifidobacterium and Lactobacillus is good in the enteron aisle; Simultaneously; Its propagation to enterococcus, enterobacteria and C.perfringens is few, and in enteron aisle, produces lower pH value.
The invention provides a kind of fermented dairy product that contains complex prebiotics and beneficial flora; It is added with lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium lactis BB-12 and lactobacillus acidophilus, has also added two kinds of prebioticses of FOS and galactooligosaccharide in the fermented dairy product; The content of stating FOS in the 1000g fermented dairy product raw material is 0.25 to 60g, and the content of galactooligosaccharide is 0.25 to 60g, and the scope of FOS and galactooligosaccharide content sum is 0.5 to 120g.
In another schematic embodiment of a kind of fermented dairy product that contains complex prebiotics and beneficial flora, the bifidobacterium lactis BB-12 content that is added is 10 5To 10 7CFU/g, the content of lactobacillus acidophilus are 10 5To 10 7CFU/g.
Also there is difference in each individuality to the tolerance of prebiotics; Use separately a kind of prebiotics, its effect amount is often bigger, possibly cause a few peoples' phenomenons such as generation diarrhoea, flatulence; And use complex prebiotics not only can reduce effect amount cost, and can avoid above-mentioned phenomenon as far as possible.
A kind of fermented dairy product that contains complex prebiotics and beneficial flora; Through external fecal bacteria culture experiment; Filter out complex prebiotics combination galactooligosaccharide (GOS) and FOS (FOS) with cooperative synergism effect; This combination is to the cultivation effect of beneficial bacterium Bifidobacterium and Bacillus acidi lactici good (12 hours countings); Simultaneously, its propagation few (12 hours counting) to enterococcus, enterobacteria and C.perfringens, galactooligosaccharide (GOS) and FOS (FOS) combination results less pH value (counting in 12 hours); The reduction of pH value reduces the growth of harmful bacterium and all plays crucial effects for the absorption of mineral matter for the health of keeping whole enteron aisle.
The specific embodiment
Embodiment 1
A kind of fermented dairy product that contains complex prebiotics and beneficial flora, its prescription be (in the components by weight percent of 1000 gram raw materials) as follows:
White granulated sugar: 75 grams;
PURE WHEY: 2 grams;
FOS: 5 grams;
Galactooligosaccharide: 5 grams;
Food additives: 5 grams;
Lactobacillus bulgaricus adds streptococcus thermophilus: 0.02U;
Bifidobacterium lactis BB-12:10 5CFU/g;
Lactobacillus acidophilus: 10 5CFU/g;
All the other are supplied with clean newborn raw milk.
Below be a kind of preparation technology who contains the fermented dairy product of complex prebiotics and beneficial flora:
1) raw milk is newborn only, obtains clean newborn raw milk;
2) batching: food additives, white granulated sugar, lactalbumin, FOS, galactooligosaccharide are mixed the back add in the clean newborn raw milk of circulation, open during the batching beginning and stir, after batching is accomplished, stop circulation, directly carry out the push pipe operation;
3) use clean newborn raw milk constant volume, obtain feed liquid;
4) to the feed liquid preheating: 65 ℃ of preheat temperatures;
5) feed liquid is outgased: degassing temperature is 65 ℃, and pressure is-90Kpa;
6) to the feed liquid homogeneous: homogenization pressure is 180bar;
7) to the feed liquid sterilization: sterilization temperature is 95 ℃, and the time is 300 seconds;
8) feed liquid cooling: be cooled to 42 ℃;
9) inoculation, fermentation: when cooled feed liquid is got into fermentation tank 1/3, start and stir, add bacterial classification in proportion, treat that charging finishes continued and stirred 10 to 15 minutes; Stop to stir, begin fermentation, fermentation temperature is 42 ℃;
10) fermentation termination is measured: fermentation termination is controlled at 75 0T obtains acidified milk;
11) cool off can: the acidified milk after acidity is touched the mark is cooled to 25 ℃, squeezes into the sour milk storage tank, and this acidified milk stirred 15 seconds after all getting into storage tank, and jam is added in the acidified milk.
Embodiment 2
A kind of fermented dairy product that contains complex prebiotics and beneficial flora, its prescription be (in the components by weight percent of 1000 gram raw materials) as follows:
White granulated sugar: 70 grams;
PURE WHEY: 2 grams;
Lactobacillus bulgaricus adds streptococcus thermophilus: 0.3U;
Bifidobacterium lactis BB-12:10 7CFU/g;
Lactobacillus acidophilus: 10 7CFU/g;
FOS: 0.25 gram;
Galactooligosaccharide: 0.25 gram;
Food additives: 3 grams;
Strawberry fruit grain: 75 grams;
All the other are supplied with clean newborn raw milk.
Embodiment 3
A kind of fermented dairy product that contains complex prebiotics and beneficial flora, its prescription be (in the components by weight percent of 1000 gram raw materials) as follows:
White granulated sugar: 70 grams;
PURE WHEY: 2 grams;
Lactobacillus bulgaricus adds streptococcus thermophilus: 0.2U;
Lactobacillus acidophilus: 10 6CFU/g;
Bifidobacterium lactis BB-12:10 6CFU/g;
FOS: 60 grams;
Galactooligosaccharide: 60 grams;
Food additives: 3 grams;
Haw berry grain: 30 grams;
Red date fruit grain: 30 grams;
All the other are supplied with clean newborn raw milk.
External fecal bacteria culture experiment
1. given the test agent: the stool suspension of taking from normal adults.
2. prebiotics preparation
Galactooligosaccharide (GOS): galactooligosaccharide content >=42%, liquid state, Dutch Friesland;
Xylo-oligosaccharide: wood sugar content >=70%, Shandong dragon power;
Inulin: inulin content >=99.5%, average degree of polymerization >=23, Belgian ORAFTI;
FOS (FOS): FOS content >=41%, Guangxi Ao Ligao.
3. culture medium
The EMB culture medium, Sodium azide-crystal violet-aesculin agar medium, the LBS agar medium, the BBL agar medium, the TSC agar medium is all purchased the Bioisystech Co., Ltd in Hai Bo.
Sugar-free GAM culture medium: soya peptone 3g, peptone 10g, digestion serum powder 13.5g, yeast extract 5g, beef extract 2.2g, beef liver 1.2g, glucose 3g, KH 2PO 42.5g, soluble starch 5g, L-cysteine salt 0.3g, sodium thioglycollate 0.3g, OX-heart soup 1000mL transfers pH7.2.
4. laboratory apparatus
The SW-CJ superclean bench, BBL anaerobic jar, anaerobism incubator, DNP-9028 constant incubator, pressuresteam sterilization pot, pH211 precision acidity meter, biochemical intelligent incubator, thermostat water bath.
5. test method is tested and is pressed table 1 grouping.
Table 1 test grouping situation.
Test group Medium component
Contrast 1 Sugar-free GAM culture medium
Contrast 2 GAM culture medium+1% glucose
Experimental group 1 GAM culture medium+1%GOS
Experimental group 2 GAM culture medium+1%XOS
Experimental group 3 GAM culture medium+1% inulin
Experimental group 4 GAM culture medium+1%FOS
Experimental group 5 GAM culture medium+1%GOS+FOS (1:1)
Experimental group 6 GAM culture medium+1%FOS+XOS (1:1)
Experimental group 7 GAM culture medium+1%GOS+XOS (1:1)
Experimental group 8 GAM culture medium+1%FOS+ inulin (1:1)
Establish 3 parallel appearance for every group.250 ml nutrient solution branches are filled to infusion bottle, jump a queue and plug syringe needle, extract syringe needle immediately after 115 ℃ of sterilization 20 min sterilization.Get fresh excreta 1g and add in the 200ml physiological saline and fully mix, get supernatant 1ml and add respectively and respectively organize in the nutrient solution 37 ℃ of cultivations.Detect and respectively to organize the quantity that nutrient solution is cultivated Bifidobacterium behind back 6h, the 12h, Bacillus acidi lactici, enterobacteria, enterococcus and C.perfringens, measure the pH of zymotic fluid simultaneously.
6. method of counting
Get zymotic fluid 1ml, add fully dilution among physiological saline 9 ml, get this liquid 1ml again, add fully dilution among the physiological saline 9ml, gradient dilution selects suitable gradient to count different gut flora quantity, operation of according to the form below method and counting.
The cultivation method of counting of the main flora of table 2 enteron aisle.
Intestinal Culture medium Condition of culture Counting
Bifidobacterium The BBL culture medium adds neomycinsulphate 100 μ g/ml, sodium propionate 15mg/ml, lithium chloride 3mg/ml Anaerobism, 37 ℃, 48h Counting white or milky bacterium colony, G +Irregular bacillus bacterium colony
Lactobacillus The LBS culture medium Anaerobism, 37 ℃, 48h White or milky bacterium colony, G +Bacillus
Enterobacteria The EMB culture medium Aerobic, 37 ℃, 24h Red bacterium colony, G —Bacillus
Enterococcus Sodium azide-crystal violet-aesculin agar medium Aerobic, 37 ℃, 24h Obvious brown haloing, G +Coccus
C.perfringens TSC culture medium+additive Anaerobism, 37 ℃, 48h The bacterium colony of black fluorescence under the ultraviolet light
7. result and analysis.
The cultivation counting (Log (cfu/mL)) of table 3 pair Bifidobacterium.
Prebiotics 0 hour 6 hours 12 hours
Contrast 1 1.60 1.85 1.60
Contrast 2 1.60 5.60 5.70
The FOS+ inulin 1.60 5.00 6.26
FOS+GOS 1.60 6.11 6.48
FOS+XOS 1.60 5.08 6.28
FOS 1.60 5.78 6.00
GOS+XOS 1.60 5.58 5.00
GOS 1.60 5.00 5.60
Inulin 1.60 5.32 5.30
XOS 1.60 5.34 5.00
The cultivation counting (Log (cfu/mL)) of table 4 pair Bacillus acidi lactici.
Prebiotics 0 hour 6 hours 12 hours
Contrast 1 4.38 4.60 4.47
Contrast 2 4.38 9.00 8.79
The FOS+ inulin 4.38 8.85 7.41
FOS+GOS 4.38 8.89 9.04
FOS+XOS 4.38 8.64 8.88
FOS 4.38 8.89 8.14
GOS+XOS 4.38 8.82 8.56
GOS 4.38 8.73 8.83
Inulin 4.38 8.51 8.00
XOS 4.38 8.48 8.49
The cultivation counting (Log (cfu/mL)) of table 5 pair enterobacteria.
Prebiotics 0 hour 6 hours 12 hours
Contrast 1 3.48 7.83 8.49
Contrast 2 3.48 8.18 8.87
The FOS+ inulin 3.48 7.30 8.40
FOS+GOS 3.48 7.90 8.15
FOS+XOS 3.48 8.28 8.20
FOS 3.48 7.97 8.32
GOS+XOS 3.48 8.48 8.48
GOS 3.48 8.16 8.26
Inulin 3.48 6.00 8.60
XOS 3.48 8.30 8.24
Table 6 pair enterococcal cultivation counting (Log (cfu/mL)).
Prebiotics 0 hour 6 hours 12 hours
Contrast 1 2.34 4.86 6.82
Contrast 2 2.34 5.70 7.05
The FOS+ inulin 2.34 5.69 7.30
FOS+GOS 2.34 5.80 6.45
FOS+XOS 2.34 5.58 6.56
FOS 2.34 5.73 7.08
GOS+XOS 2.34 5.67 6.58
GOS 2.34 5.81 6.52
Inulin 2.34 4.80 7.45
XOS 2.34 5.71 6.57
The cultivation counting (Log (cfu/mL)) of table 7 pair C.perfringens.
Prebiotics 0 hour 6 hours 12 hours
Contrast 1 2.70 3.85 7.40
Contrast 2 2.70 3.90 6.00
The FOS+ inulin 2.70 3.88 6.85
FOS+GOS 2.70 4.52 5.90
FOS+XOS 2.70 4.36 6.20
FOS 2.70 4.31 6.04
GOS+XOS 2.70 4.58 6.30
GOS 2.70 4.83 6.22
Inulin 2.70 4.60 7.00
XOS 2.70 5.00 6.60
Table 8 combination of prebiotics fermentation back is to the influence of pH value.
Prebiotics 6 hours 12 hours
Contrast 1 6.78 6.43
Contrast 2 5.57 4.42
The FOS+ inulin 6.59 4.87
FOS+GOS 6.04 4.67
FOS+XOS 6.31 4.65
FOS 6.73 5.57
GOS+XOS 6.01 4.73
GOS 6.14 4.77
Inulin 6.14 4.71
XOS 7.06 6.25
The result shows; GOS and FOS combination are good to the cultivation effect of beneficial bacterium Bifidobacterium and Lactobacillus; Simultaneously, few to the propagation of enterobacteria, enterococcus and C.perfringens, GOS and FOS combination results lower pH value; The reduction of pH value reduces the growth of harmful bacterium and all plays crucial effects for the absorption of mineral matter for the health of keeping whole enteron aisle.Therefore, confirm that GOS and FOS are optimum combination of prebiotics.The combination of GOS and FOS surpasses uses FOS and GOS separately, explains that there is synergistic function in the combination of FOS and GOS.Therefore, use GOS and FOS, can effectively solve the FOS that uses single high dose and phenomenons such as the exhaust that brings, diarrhoea, reached the effect of higher single prebiotics with lower complex prebiotics amount, and reduced the effect cost.
It will be understood by those skilled in the art that for count value in table 3 to the table 7 and the pH value in the table 8, is reference with 12 hours point data mainly.
In this article, " schematically " expression " is served as instance, example or explanation ", should any diagram, the embodiment that be described to " schematically " in this article be interpreted as a kind of preferred or have more the technical scheme of advantage.
Be to be understood that; Though this specification is described according to each embodiment; But be not that each embodiment only comprises an independently technical scheme, this narrating mode of specification only is for clarity sake, and those skilled in the art should make specification as a whole; Technical scheme among each embodiment also can form other embodiments that it will be appreciated by those skilled in the art that through appropriate combination.
The listed a series of detailed description of preceding text only is specifying to feasibility embodiment of the present invention; They are not in order to restriction protection scope of the present invention, allly do not break away from equivalent embodiment or the change that skill of the present invention spirit done and all should be included within protection scope of the present invention.

Claims (2)

1. fermented dairy product that contains complex prebiotics and beneficial flora; It is added with lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium lactis BB-12 and lactobacillus acidophilus, it is characterized in that: also added two kinds of prebioticses of FOS and galactooligosaccharide in the said fermented dairy product; The content of FOS described in the said fermented dairy product raw material of every 1000g is 0.25 to 60g, and the content of said galactooligosaccharide is 0.25 to 60g, and the scope of said FOS and said galactooligosaccharide content sum is 0.5 to 120g.
2. fermented dairy product as claimed in claim 1, the said bifidobacterium lactis BB-12 content that is wherein added is 10 5To 10 7CFU/g, the content of lactobacillus acidophilus are 10 5To 10 7CFU/g.
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CN102845518A (en) * 2012-10-17 2013-01-02 石家庄君乐宝乳业有限公司 Yoghourt with health-care function, as well as preparation method and application thereof
CN104186655A (en) * 2014-08-27 2014-12-10 内蒙古伊利实业集团股份有限公司 Low-fat fermented milk having blood fat reduction and anti-oxidization functions and preparation method thereof
CN104719475A (en) * 2015-02-11 2015-06-24 江门市德昂生物科技有限公司 Composition for producing dairy products and production method of dairy products
TWI554214B (en) * 2013-02-08 2016-10-21 愛之味股份有限公司 Process for preparing a functional fermented milk product with low acidity and containing a low content of lactose and enhanced with galactooligosaccharides and bifidobacterium, and functional fermented milk product prepared therewith
CN106819107A (en) * 2016-12-29 2017-06-13 石家庄君乐宝乳业有限公司 Leben with the effect of whole intestines and preparation method thereof
CN110710571A (en) * 2019-11-25 2020-01-21 上海昊岳食品科技有限公司 Functional yoghourt and preparation method thereof
CN111011866A (en) * 2019-12-12 2020-04-17 上海理工大学 Application of prebiotics in promoting growth of lactobacillus plantarum in intestinal environment
CN111487332A (en) * 2019-11-08 2020-08-04 天津科技大学 Evaluation method and application of prebiotics effect of fructo-oligosaccharide with single polymerization degree
CN112741334A (en) * 2019-10-29 2021-05-04 内蒙古伊利实业集团股份有限公司 Prebiotic compositions, and uses thereof
CN112998276A (en) * 2021-02-01 2021-06-22 天津科技大学 Synbiotic compound preparation for preventing diarrhea, intestinal leakage and sepsis caused by excessive ingestion of prebiotics, preparation method and application

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Publication number Priority date Publication date Assignee Title
CN102845518A (en) * 2012-10-17 2013-01-02 石家庄君乐宝乳业有限公司 Yoghourt with health-care function, as well as preparation method and application thereof
TWI554214B (en) * 2013-02-08 2016-10-21 愛之味股份有限公司 Process for preparing a functional fermented milk product with low acidity and containing a low content of lactose and enhanced with galactooligosaccharides and bifidobacterium, and functional fermented milk product prepared therewith
CN104186655A (en) * 2014-08-27 2014-12-10 内蒙古伊利实业集团股份有限公司 Low-fat fermented milk having blood fat reduction and anti-oxidization functions and preparation method thereof
CN104186655B (en) * 2014-08-27 2016-06-29 内蒙古伊利实业集团股份有限公司 There is low-fat yogurt of blood fat reducing and anti-oxidation function and preparation method thereof
CN104719475A (en) * 2015-02-11 2015-06-24 江门市德昂生物科技有限公司 Composition for producing dairy products and production method of dairy products
CN106819107A (en) * 2016-12-29 2017-06-13 石家庄君乐宝乳业有限公司 Leben with the effect of whole intestines and preparation method thereof
CN112741334A (en) * 2019-10-29 2021-05-04 内蒙古伊利实业集团股份有限公司 Prebiotic compositions, and uses thereof
CN111487332A (en) * 2019-11-08 2020-08-04 天津科技大学 Evaluation method and application of prebiotics effect of fructo-oligosaccharide with single polymerization degree
CN110710571A (en) * 2019-11-25 2020-01-21 上海昊岳食品科技有限公司 Functional yoghourt and preparation method thereof
CN111011866A (en) * 2019-12-12 2020-04-17 上海理工大学 Application of prebiotics in promoting growth of lactobacillus plantarum in intestinal environment
CN111011866B (en) * 2019-12-12 2023-03-24 上海理工大学 Application of prebiotics in promoting growth of lactobacillus plantarum in intestinal environment
CN112998276A (en) * 2021-02-01 2021-06-22 天津科技大学 Synbiotic compound preparation for preventing diarrhea, intestinal leakage and sepsis caused by excessive ingestion of prebiotics, preparation method and application
CN112998276B (en) * 2021-02-01 2023-08-29 天津科技大学 Synbiotic compound preparation for preventing diarrhea, intestinal leakage and sepsis caused by excessive intake of prebiotics, preparation method and application

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