CN102519927A - Multichannel fluorescence test system and method of water concentration field - Google Patents
Multichannel fluorescence test system and method of water concentration field Download PDFInfo
- Publication number
- CN102519927A CN102519927A CN2011104084835A CN201110408483A CN102519927A CN 102519927 A CN102519927 A CN 102519927A CN 2011104084835 A CN2011104084835 A CN 2011104084835A CN 201110408483 A CN201110408483 A CN 201110408483A CN 102519927 A CN102519927 A CN 102519927A
- Authority
- CN
- China
- Prior art keywords
- probe
- concentration
- tracer
- light
- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to multichannel fluorescence test system and method of a water concentration field. Adopting the principle (LIF) (laser induced fluorescence) that a tracer can generate fluorescence under the irradiation of a specific light source, the system includes: a tracer, fluorescein sodium, put in a water area, and probe groups set at each detecting spot in the water area. The probes are connected to collection boxes through shield cables, and the collection boxes are then connected to a central measurement and control computer equipped with collection and calibration software through a computer bus or communication network for conducting sampling, analysis and display on the tracer concentration of discharged sewage in a large water area. The important components, probes, include LEDs (light-emitting diodes) irradiating water currents containing the tracer, and photocells for receiving excited fluorescence in the water. The system and method of the invention fill the gap in the detection field of pollutant concentration fields in large water physical model experiments, and are also effective means for field observation of water pollution.
Description
Technical field
The present invention relates to the hyperchannel fluorometric investigation system and method for a kind of waters concentration field, is a kind of test macro and method of utilizing bussing technique or communication network technology water field of big area tracer concentration field to be carried out light, detection of electrons.
Background technology
More and more important in the water environment protection research in modern times of the concentration of certain material of test in the large tracts of land concentration field.For example occur possible pollution source on certain position of certain river basins, how to judge of the pollution effect of these pollution source, need carry out the understanding of the concentration field of pollutant hydrodynamic force dilution the influence section to the basin, downstream.Research means can take in the model of the section of physical model, to be provided with the mode of test macro; The mode of also can be in natural water area carrying out prototype measurement with test macro.
For example, at present in nuclear power plant's environmental pollution prediction research, the low-activity liquid efflunent formed concentration field in the waters that requires its physical experiments to simulate to discharge with nuclear power plant's warm water discharge.Adopt certain tracer agent to simulate the hydrodynamic force dilution of low-activity effluent in the flow field, waters of being discharged in the test.For obtaining the simulated concentration field, need to gather the instrument and equipment (test macro) of this tracer concentration in the current.Because the area in modeling waters is very huge, the area of concentration field to be measured is no less than thousands of square metres.Obtain its concentration field, must use probe to mine massively and collect the concentration on the discrete point position, data synthesize large-area concentration field again.If test macro is set in natural water area, the area in waters is more vast, can increase probe quantity according to needs.
The principle of fluoroscopic examination all has application in the detecting instrument of chemical industry and pharmaceuticals industry, but is mostly the fluorescence with the generation of LIF material, and is single sonde-type detecting instrument.In the water environment scientific research, once occurred inducing fluorescent material in the water to send the concentration field pick-up unit of fluorescence, but the area of this device checkout area is difficult to surpass 1m with the long laser light sheet of a certain standing wave
2Water surface area to greater than hundreds of square metres does not have possibility extremely.LASER Light Source is very expensive, in the detection of large tracts of land concentration field, is difficult to use.
Based on above reason, detectable concentration field in the large tracts of land flow field, be faced with how to gather, the problem of storage and concentration of treatment field data.
Summary of the invention
In order to overcome prior art problems, the present invention proposes the hyperchannel fluorometric investigation system and method for a kind of waters concentration field.Described system and method is the probe that adopts based on monochromatic light induced fluorescence detectable concentration (LIF) principle, to obtain the concentration parameter of discrete point.Through computer bus or communication network technology, hundreds of measuring points are coupled together.Through computer bus or communication network with the data delivery of gathering in central observing and controlling computing machine, form complete concentration field after handling discrete data.
The objective of the invention is to realize like this: the hyperchannel fluorometric investigation system of a kind of waters concentration field; Comprise: the tracer agent of in the waters, throwing in; Be arranged on the probe crowd that each sensing point is made up of a plurality of probes in the waters; Described probe is connected with vasculum through shielded cable; Described vasculum is connected with the central observing and controlling computing machine of calibration software with being equipped with to gather through computer bus or communication network, and described probe comprises and excites the LED of said tracer agent generation fluorescence to manage, and receives the photoelectric cell of said fluorescence.
A kind of method of using said system to carry out the hyperchannel fluorometric investigation of waters concentration field, the step of said method is following:
Throw in the step of tracer agent: be used for the release position selected, throw in the tracer agent of preset concentration in tested waters;
The flow through step of probe of tracer agent: the current that are used to the to carry tracer agent measurement zone of each probe of flowing through;
Detect the step of tracer agent in the current: the current of the tracer-containing of the measurement zone that is used to flow through are by the irradiation of LED pipe; Excite generation fluorescence; Photoelectric cell in the probe converts said fluorescence into the analog quantity current signal and transfers to vasculum, and I/V and A/D conversion via vasculum become the digital voltage parameter;
The step of transmission: be used for vasculum the digital voltage parameter is transferred to central control computer through computer bus or communication network;
The step of circle collection data: be used for each probe of central observing and controlling computing machine scan round, gather the digital voltage parameter that stores each probe;
Calculate the step of tracer concentration: be used for the characterisitic parameter of central observing and controlling computing machine, go out the tracer concentration value of respectively popping one's head in measured by the measured digital voltage calculation of parameter of each probe according to " concentration-voltage " linear equation of each probe configuration file;
Below two steps run parallel:
The step that shows: be used at central observing and controlling computer screen; The interface that shows institute waters of surveying and measuring point thereof layout simulation drawing, the current tracer concentration value of respectively popping one's head in measured of demonstration and on each probe positions with the tracer concentration value symbol of gray scale visual representation;
The step that stores: behind the circle collection, will work as the tracer concentration value storage that time each of collection popped one's head in and surveyed, and fill in the data form.
A kind of method of demarcation probe of above-mentioned test macro, the step of said method is following, and the described method of this step is the demarcation to single probe:
The step of preparation volumetric solution: be used to prepare the tracer agent that is no less than 10 kinds of variable concentrations and demarcate sample;
Obtain the step of series analog voltage parameter: be used to obtain each probe the variable concentrations tracer agent is demarcated the series analog voltage parameter that sample is exported,, obtain a series of voltage parameter values promptly with respect to a series of known concentration values;
Draw the step of " concentration-voltage " curve map: be used to utilize a series of concentration known values and corresponding a series of voltage parameter values obtained, draw out " concentration-voltage " curve;
Obtain the step of " concentration-voltage " linear equation: be used for going out " concentration-voltage " linear equation, and then obtain the characterisitic parameter of " concentration-voltage " linear equation: slope K and pitch a according to " concentration-voltage " curve fitting.
The beneficial effect that the present invention produces is: the principle that the present invention is based on monochromatic light induced fluorescence detectable concentration (LIF); Be implemented in the detection of water field of big area utilization probe crowd, and the detection probe of utilizing communication network or computer bus technology will be arranged on water field of big area is connected with central observing and controlling computing machine to the formed concentration field of sewer of tracer-containing solution.Through central observing and controlling computing machine the tracer concentration of water field of big area is sampled, shows, analyzed, solved the concentration field test problem in large tracts of land flow field.Described probe uses cheap LED pipe and photoelectric cell, has taked the measure of a series of discharge surround lightings and environment air interference ripple noise, and the concentration parameter that is obtained is accurate.Described system architecture is simple, and is with low cost, uses limited research funding, effectively solved the detection problem of large tracts of land concentration field.This system and method has been filled up the blank of relevant pollutant hydrodynamic force dilution diffusion concentration field detection range in the physical experiments of large-scale waters, and the environmental impact research to the low level waste water discharging of current nuclear power plant has realistic meaning; In addition, this system and method also is the effective means of water pollution field field observation.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is described further.
Fig. 1 is the systematic schematic diagram of embodiments of the invention one said test macro;
Fig. 2 is the structural representation of embodiments of the invention two said vasculums;
Fig. 3 is embodiments of the invention three said sonde configuration synoptic diagram;
Fig. 4 is light, the circuit theory diagrams of embodiments of the invention three said probes;
Fig. 5 is the synoptic diagram of embodiments of the invention four said probe isolating caps, is the enlarged drawing of A position among Fig. 3.
Embodiment
Embodiment one:
Present embodiment is the hyperchannel fluorometric investigation system of a kind of waters concentration field, and is as shown in Figure 1.Present embodiment comprises: the tracer agent of in the waters, throwing in; Be arranged on the probe crowd that each sensing point is made up of a plurality of probes in the waters; Described probe is connected with vasculum through shielded cable; Described vasculum is connected with the central observing and controlling computing machine of calibration software with being equipped with to gather through computer bus or communication network, and described probe comprises and excites the LED of said tracer agent generation fluorescence to manage, and receives the photoelectric cell of said fluorescence.System shown in Figure 1 is five vasculums, and each vasculum connects the synoptic diagram of three probes.Present embodiment is actual can to connect nearly 10 or more vasculums, and each vasculum connects 24 or more probe.
The described waters of present embodiment comprises two different concept: the simulation waters that at first is large-scale waters physical experiments; Next is the natural water area in actual river, river, lake, sea.Described large-scale waters physical model can effective natural water area streamflow regime that is virtually reality like reality.
The described tracer agent of present embodiment be a kind of in current the chemical reagent of representative simulation emission.Utilize this chemical reagent to replace pollutant to throw in actual waters or the upper reaches in simulation waters; Pick-up unit is set in downstream detects the concentration of these tracer agents in the current of downstream, draw out the concentration profile in whole waters according to the coordinate of detectable concentration point position then.Present embodiment has selected uranin as tracer agent, and except that following condition, also because of can going out fluorescence with the optical excitation of blue led pipe, and the use photoelectric cell picks up the fluorescence that it sends.
The selection of tracer agent is based on following condition: water-soluble, harmless, temperature coefficient is little, to Gu Bi do not have adsorbability, consumption is minimum, so that the unlikely temperature difference (10 ℃) the density current hierarchical nature that influences of its WS.The selected uranin of present embodiment meets these requirements basically.
The described probe of present embodiment is a kind of tracer agent fluorescence and device that picks up fluorescence of exciting based on the LIF principle.Therefore, probe comprises two meat and potatoes: radiating portion and receiving unit.
Based on the detecting instrument of LIF principle, its radiating portion uses LASER Light Source or thermal light source usually, like tungsten bulb; Glow discharge spot lamp, mercury bulb etc.Because present embodiment has hundreds of probes, at first can not adopt expensive Laser Devices; Secondly, can not adopt the bigger thermal light source of volume etc., disturb water movement to cause probe.Basic demand to excitation source is the light that sends the specialized narrowband spectrum that conforms to the tracer agent exciting light, and laser instrument meets the requirements most, but too expensive; It is relatively poor that LED manages the coherence of relative LASER Light Source, but economic performance is fine.Particularly the LED of emerging super brightness manages in recent years, and monochromaticity is better, and is very cheap again, meets the requirement that the hundreds of probes of the said system of present embodiment are made.
The effect of receiving unit is to receive the fluorescence that tracer agent sent.Its basic demand is responsive to received fluorescence wave band, gets rid of the interference of other light, the correct fluorescence that picks up.The element that receives fluorescence can have multiple choices; The general expensive device of using photomultiplier, avalanche diode etc. to have very big enlarging function more; And phototriode, photodiode (photoelectric cell) etc. seldom adopt, and photoelectricity amplifies a little less than effect compares very, but cheap.It is suitable selection.
Because the veiling glare of the same wavelength of fluorescence that is no lack of in the environment and is excited exists,, must get rid of the interference of ambient light in conventional environment for the said system of present embodiment can be used.Present embodiment advances the light side at photoelectric cell the logical type optical filter of band is set, the light that only allows the fluorescence wave band through and filter out heterochromatic light.
Detect accurately for making, present embodiment is provided with isolating cap in probe, makes in the irradiation of light and the current scope that reception is confined to a fixed volume, only detects the tracer concentration in this piece water body.Concrete grammar is: apart from the about 1cm of probe rod end face place one conical spacer is being set, this sheet is connected with the set collar of probe rod termination with 3 poles, to constitute the about 1cm under the probe end face
3Measurement volumes.Because conical surface can reflex to exciting light beyond the probe end face, does not receive in the light fibre bundle and can not get into, and can lower the interference of exciting light to fluorescence signal, especially when receiving loop optical filtering imperfection.
Described vasculum provide LED pipe power supply, the analog quantity current signal of photoelectric cell output is carried out I/V conversion and A/D conversion, and send into communication network or computer bus transfers to central observing and controlling computing machine.
In order to get rid of the interference of surround lighting to fluoroscopic examination; So that the said system of present embodiment can be applicable in the ordinary ray environment; Must adopt modulation and demodulation system circuit, so that from numerous and diverse background signal, extract and amplify faint fluorescence signal, the antijamming capability of raising system.Concrete grammar is: the power supply to the LED pipe carries out frequency modulation (PFM), makes LED pipe exciting light with the tracer-containing current in the modulating frequency irradiation measurement zone.The signal of photoelectric cell output is delivered to demodulator circuit, after demodulation is amplified, become with fluorescence electric signal same frequency, corresponding to the electric signal of tracer concentration.So just, get rid of all outer background noises, kept useful fluorescence information.
The electric signal of the tracer concentration that probe obtains needs to transfer to central observing and controlling computing machine through computer bus or communication network.In the physical model of large-scale waters, the bussing technique that can directly use a computer connects vasculum and central observing and controlling computing machine.For example use transmission range RS485 industry data bus far away, send into central observing and controlling computing machine through RS485-RS232 converter again.To natural water areas such as river, river, lake, seas, then need be connected with central observing and controlling computing machine through cordless communication network or Inter net.The probe battle array of promptly arranging at a detection zone can be connected on one or more vasculums, and vasculum is connected with public communication network through router, also can be connected with the communication network of special use.No matter be public communication network or special-purpose communication network, can carry out communication and data transmission through wired or wireless mode.At central observing and controlling computing machine one end, also connect a router.Central authorities' observing and controlling computing machine can use special-purpose or general network software and vasculum carries out communication and exchanges data.The connected mode of communication network can have the place of network to realize any, does not receive the restriction of region fully.
The described central observing and controlling computing machine of present embodiment can be a common PC, or server.Acquisition software and calibration software wherein have been installed.Described acquisition software is worked out according to the embodiment of the invention seven, eight, nine described " hyperchannel fluorescence concentration field method of testing ".Described method of testing is used the described system of present embodiment, has realized the test of fluorescence concentration field.
Described calibration software is to work out according to the embodiment of the invention ten described " demarcating the method for probe ".To confirm the characterisitic parameter of each probe.
Embodiment two:
Present embodiment is the improvement of embodiment one, is the refinement of embodiment one about vasculum, and principle is as shown in Figure 2.Be provided with power module, communication interface and a plurality of acquisition module in the described vasculum of present embodiment, described acquisition module contains the I/V converter that is connected with probe, and said I/V converter selects a switch, A/D converter and communication interface to be connected with eight successively.
The described vasculum of present embodiment is the crucial intermediate link that probe is connected with central observing and controlling computing machine.A plurality of acquisition modules and a power module can be housed in each vasculum, are three acquisition modules among Fig. 2, in actual the enforcement more acquisition module can also be installed.Power module is that each probe and each acquisition module that are connected provide constant voltage source.Each acquisition module can connect a plurality of probes, and Fig. 2 is illustrated as three probes, can connect 24 or more probes in actual the use.The output electric signal of probe is delivered to communication interface after I/V conversion and A/D conversion, and through computer bus or communication network electric signal is transferred to central observing and controlling computing machine.Each vasculum has the independent numbering of oneself; Each acquisition module has unique address code; And each probe has unique port number at the interface of adopting on the cabinet (port), so that the identification of central observing and controlling computer addressing.
Embodiment three:
Present embodiment is the improvement of embodiment one, two, is the refinement of embodiment one, two about probe, and structure is as shown in Figure 3.The described probe of present embodiment comprises: LED pipe 2 and photoelectric cell 9 are installed in the box body 4, described box body, and are connected with signal circuit board 11, signal circuit board is connected with vasculum through joint 12.Said photoelectric cell advances the light side and is provided with filter set 8; Be provided with before the described filter set and receive light fibre bundle 7; Described LED pipe bright dipping side is provided with exciting light optical fiber bundle 3, and described reception light fibre bundle and exciting light optical fiber bundle place probe rod tube 5, and the probe rod pipe end is connected with isolating cap 6.Described LED pipe is electrically connected with modulator and constant current source, and said constant current source is electrically connected with the power module of vasculum; Described photoelectric cell is electrically connected with AC amplifier, detuner, direct current amplifier successively, and the output of probe is electrically connected with I/V converter in the vasculum.Said modulator, constant current source, AC amplifier, detuner, direct current amplifier all are included in the signal circuit board 11.Photoelectric cell is connected with signal circuit board through cable 10, and the LED pipe is connected with signal circuit board through cable 1.
The light of present embodiment, electrical schematic diagram, as shown in Figure 4, wherein the dotted arrow line is represented light path, solid arrow line indication circuit.
Be guiding exciting light and reception light, present embodiment has adopted the only fibre bundle of tens μ m of single diameter.Sending out, receive optical fiber, to be made into optical fiber right.The launching fiber Shu Yiduan of exciting light and the coupling of LED pipe; Fluorescence receives fibre bundle one end and then is coupled with filter set.Numerous optical fiber pierce in the probe rod tube of D10mm random alignment.With meticulous the polishing in rod tube termination of filling full fibre bundle, guarantee the light perpendicular end surface of coming in and going out.The about at interval 1cm of probe rod end face and spacer, this is the passages of current through measuring body.
Modern optical fiber technology makes the transmission loss of light in optical fiber very little, therefore, can think, it is minimum in the transmission loss (TL) of lacking in (about 30cm) optical fiber to receive light, can give basically the loss of sensed light signal and disregarding.
Exist Stokes shift between emission light and the exciting light.The wavelength of fluorescence is always greater than exciting light wavelength.The exciting light center light spectral frequency of present embodiment is 488 nanometers, and the center light spectral frequency that receives light is 520 nanometers.
With LED is exciting light sources, meets requirement cheaply.Select the large-power light-emitting diodes LED (3 watts) that has heat radiator for use,, must select blue light (wavelength 488 nanometers) type for use, model: GT-P03B3410340 to the uranin tracer agent.Adopt constant current source power supply, insert big current-limiting resistance, reduce the supply current of LED; And on the current-limiting resistance of LED and connect the thermistor of negative temperature coefficient, the LED supplying electric current is stablized in compensation caused supply current drift because temperature rises.
Adopting photoelectric cell to receive fluorescence signal, also is to follow principle cheaply.Photoelectric cell is selected model for use: BPW21R, purplish blue photoelectric cell (6 * 6mm) emitting fluorescences to Fluress (green-yellow light of 520nm) sensitivity.Use the logical type filter glass of two optical filter: 510nm (yellow) cut-off type filter glass and 520nm ± 5nm (green) band.After the stack of two filter glasss, only the luminous energy of 510nm-525nm wavelength is sent on the photoelectric cell.
Be provided with modulator on the constant current source of LED pipe, in the Signal Processing process that photoelectric cell obtains, be provided with detuner.Improve antijamming capability through modulation and demodulation system, make this device can be applicable to natural light or artificial light sources environment.
The electrical signal that photoelectric cell obtains will be passed through earlier to exchange and amplified, and carries out direct current after the demodulation again and amplifies.After the amplification, export with current system.
Embodiment four:
Present embodiment is the improvement of embodiment three, is the refinement of embodiment three about isolating cap, and is as shown in Figure 5.The described isolating cap of present embodiment is provided with and is fixed in the terminal hatband 601 of probe rod tube; Described hatband is divided equally fixing three isolating cap poles 602 by circumference; The other end of pole is promptly fixing with spacer 603; Described spacer is the circular conical surface of 100 °～120 ° of drift angles, is measurement zone 604 between described hatband and the spacer.
It is terminal that said hatband is fixed in the probe rod tube, and its lower limb flushes with probe rod pipe end face.The direction of B-B flows through measurement zone among tested current such as Fig. 5, the water body in the blue light light beam of the end face 605 outgoing irradiation measurement zone, and the plain sodium of fluorescence excitation sends fluorescence.Fluorescence signal is received the light fibre bundle and is transmitted to filter set and photoelectric cell.Water body to be detected only limits to the water body in the probe measurement district.Spacer is the circular conical surface that probe rod end face center is aimed on the pinnacle, 100 °～120 ° of drift angles, and the surface black plastic-blasting, reflective to reduce.Excitation light irradiation is to spacer surface, and the measurement zone that is reflected out can not disturb the reception of fluorescence signal, and this is particularly important for the not good situation that filters.
Embodiment five:
Present embodiment is the improvement of embodiment four, is the refinement of embodiment about tracer agent.The described tracer agent of present embodiment is a uranin.
Uranin (C
20H
10NA
2O
5), molecular weight 376.28, CAS number: 518-47-8.Exciting light centre wavelength: 488nm (blue light); Fluorescence centre wavelength: 520nm (green-yellow light).
Test consumption ≯ 0.03PPM can know through computational analysis, and the caused water-mass density of temperature change that the change of caused warm water discharge density only is equivalent to for ten thousand/several years changes, and is suitable for the spike in the temperature difference density current.
The water body of the different temperatures because the current of taking this tracer agent can be flowed through (10 ℃ temperature ranges in); Thereby require the temperature coefficient of the tracer agent WS very little; In the said temperature variation range, the concentration of Fluress is diluted by hydrodynamic force mainly, and extremely low with the degree of correlation of temperature variation.
Assert through experiment: the fluorescence intensity and the concentration of the uranin WS in certain low strength range are linear, and the selection of above-mentioned test consumption meets this principles and requirements.
Embodiment six:
Present embodiment is the improvement of embodiment five, is embodiment five refinements.The emission wavelength of the described LED pipe of present embodiment is the blue light of 488 nanometers; Described photoelectric cell is responsive to 520 nanometer green-yellow lights, and described filter set can be through the light of 510-525 nanometers.
The equal coincidence detection of characteristic of the selected LED pipe of present embodiment, photoelectric cell, filter set is as the requirement of the uranin of tracer agent.
Embodiment seven:
Present embodiment is the method that a kind of embodiment of use six said systems carry out the hyperchannel fluorometric investigation of waters concentration field.Of embodiment five, the employed tracer agent of present embodiment is a uranin.
The ultimate principle of present embodiment is: at the upper reaches, waters or the freeing port place decanting point of tracer agent is set, place probe in concentration point to be measured position.The fluorescence that sends after uranin is excited in each perception water of popping one's head in converts electric signal to and transfers to central observing and controlling computing machine.Computing machine calculates corresponding concentration according to the dependent equation of voltage signal～concentration, and gives demonstration and preservation.This tracer agent only can be simulated the hydrodynamic force dilution diffusion formed concentration field of dirt in water body, other characteristic of actual dirt, as self damping, with water in other substance reaction and the concentration that causes changes, can not simulate.
The concrete steps of the said method of present embodiment are following:
The flow through step of probe of tracer agent: the current that are used to the to carry tracer agent measurement zone of each probe of flowing through.No matter be in prototype waters or the model, all the uranin WS with suitable concn injects floss hole, flows into the waters with original discharging modes together in company with draining.Discharging modes generally have: continuous blow-down or intermittent discharge.
Detect the step of tracer agent in the current: the current of the tracer-containing of the measurement zone that is used to flow through are by the irradiation of LED pipe; Excite generation fluorescence; Photoelectric cell in the probe converts said fluorescence into the analog quantity current signal and transfers to vasculum, and I/V and A/D conversion via vasculum become digital voltage signal.The key link that detects is: the demodulation behind the frequency modulation (PFM) of LED power supply, the filtering of accepting light signal, the photoelectric cell detecting signal is amplified or the like; Detection to atomic hypofluorescence signal is the bigger technology of difficulty; For ensureing the anti-interference and stable of detecting signal, circuit design parameter must be confirmed through repeated calculation and test.
The step of transmission: be used for vasculum the digital voltage parameter is transferred to central control computer through computer bus or communication network.This step is that the electric signal that vasculum obtains is transferred to central observing and controlling computing machine.Its mode can be a computer bus, promptly uses the longer distance of industrial bus standard RS485 transmission earlier, converts RS232 into through converter again, docks with universal computer interface.Or the mode of employing communication network, promptly, make each vasculum become a node of network through connecting router.Its transmission manner is abundanter, both can use special-purpose network, also can use public communication network.The bussing technique that uses a computer transmits, and is applicable in model test.Because the transmission range of RS485 rice up to a hundred are even if thousands of square metres model uses the data bus of RS485 also can well work.The transmission mode of communication network is applicable to the detection in actual river, river, lake, sea.
The step of circle collection data: be used for each probe of central observing and controlling computing machine scan round, gather the digital voltage that stores each probe.Central authorities' observing and controlling computing machine scanned all probes (quantity can reach 200) in 5 seconds, obtained the voltage signal of each probe output, calculated concentration and storage, accomplished one and gathered circulation.
Calculate the step of tracer concentration: be used for the characterisitic parameter of central observing and controlling computing machine, go out the tracer concentration value of respectively popping one's head in measured by the measured digital voltage calculation of parameter of each probe according to " concentration-voltage " linear equation of each probe configuration file.The characterisitic parameter of " concentration-voltage " linear equation refers to K (slope), two coefficients of a (pitch) of linear equation.
Below two steps run parallel:
The step that shows: be used at central observing and controlling computer screen; Show one the simulation drawing arranged of the waters of surveying and measuring point thereof, on each probe positions, can show current tracer concentration value of respectively popping one's head in measured and with the tracer concentration value symbol of gray scale visual representation.The display mode that is present embodiment has two kinds: a kind of is to show with numerical value, a kind ofly shows with gray scale.
The step that stores: behind the circle collection, will work as the tracer concentration value storage that time each of collection popped one's head in and surveyed, and fill in the data form.Each time image data derives with the Excel data form.
Embodiment eight:
Present embodiment is the improvement of embodiment seven, is that embodiment seven is about detecting the refinement of the step of tracer agent in the current.
The principle of present embodiment is: the light of the 488nm wavelength that brightness blue light LED sends, penetrate from the probe rod end face through light transmitting fiber.When current flow through measuring body, the uranin in the blue-light excited current of 488nm produced the yellow-green fluorescence of wavelength 520nm.Fluorescence is sent to filter set (510nm and 520nm) through light transmitting fiber, and (6 * 6mm), photronic electric signal exports vasculum to behind narrow-band filtering, to reach silicon purplish blue photoelectric cell.
Under finite concentration and certain light intensity, Fluress concentration is directly proportional with the fluorescence light intensity of being excited afterwards to produce.So the photoelectric cell output electric signal that present embodiment records is corresponding one by one with concentration.For obtaining the concentration on certain some position in the flow field, during measurement certain spatial resolution should be arranged.In view of the fluorescence of being excited to produce in the current possibly be a light beam, for obtaining the measurement volumes of about 1cm height fluorescence post, the 1cm place adds the about 120 ° circular conical surface shape isolating cap of a drift angle under the probe rod end face, to realize the positioning function of probe measurement.Isolating cap is set, is intended to make its dip plane reflection exciting light (blue light), it is no longer got into receive light optical fiber, interferometry (preventing that the optical filter filter effect is not good).For preventing that blue light and green-yellow light are to the interference of signal to be detected in the surround lighting, LED adopts warbled square-wave pulse constant current source power supply, and it is unaffected that present embodiment is used in conventional environment.
Because measurement that present embodiment carried out is to survey atomic weak fluorescence, be processing to the processing of fluorescence signal to atomic weak signal, its difficulty is bigger.Key is how to eliminate ground unrest, otherwise atomic weak signal is flooded by noise, can't record.Present embodiment utilization emission light and the modulation, the rectification that receive light, and the interference of removing heterochromatic light of method such as optical filter.
The step of tracer concentration comprises following substep in the described detection current of present embodiment:
The substep that light source is modulated: be used for the constant current source of LED pipe power supply is carried out frequency modulation (PFM), make the LED pipe luminous according to the frequency of modulation.Therefore, at first to choose appropriate modulating frequency.
The substep of irradiation tested water body: be used for LED and manage the light that sends has tracer agent through fiber-optic illuminated measurement zone tested water body.
The be excited substep of fluorescence of tracer agent: be used for the illuminated optical excitation of tested water body tracer agent, send fluorescence.
Receive the substep of fluorescence: be used for fluorescence that tracer agent sends through optical fiber, and see through filter set filtering heterochromatic light and reach photoelectric cell, photoelectric cell produces analog current signal.
Amplify and the rectification substep: be used for converting the analog current signal that photoelectric cell produces into analog voltage signal through I/V, rectification after exchanging amplification, the interference of removing heterochromatic light is carried out DC voltage again and is amplified.
The substep of digitized processing: be used for the analog voltage signal after amplifying through DC voltage through the digital electric signal of A/D conversion process for suitable computer bus transmission or communication network transmission.
Embodiment nine:
Present embodiment is the improvement of embodiment eight, is the refinement of embodiment eight about modulating frequency.Modulating frequency described in the substep that the described light source that LED is managed of present embodiment is modulated is 20-28 hertz.
20-28 hertz of these frequency ranges confirm that frequency range can go up from hundred K Hz, down to several Hz after through tens of kinds of frequency tests.Go through 1K that the several months has a surplus test repeatedly to number KHz range of choice (this is the habitual frequency range of modulation), still must not good fruit.The custom thinking is broken through in the back, frequency is dropped to below the 1K, again through long period test, 24 hertz of modulating frequencies of seeking preferablely eventually.This frequency is effectively in numerous and diverse background signal, with the faint fluorescence electric signal proposition of high s/n ratio.
Embodiment ten:
Present embodiment is a kind of method of demarcation probe of embodiment six said test macros.The purpose of the said method of present embodiment is: K (slope), two coefficients of a (pitch) of confirming single probe " concentration-voltage " straight-line equation.In the laboratory, probe is demarcated.In the concentration range of setting, differential by basic uniform concentration, the demarcation samples at different levels of making.
As: use 10 to demarcate samples, concentration range be 0.001-0.04 each to demarcate the concentration of sample differential be 0.004, then demarcate samples and be for ten: 0.001,0.004,0.008 ... 0.036,0.04 totally nine differential.To pop one's head in and put into each demarcation sample respectively, the actual measurement probe is demarcated the electric signal output in the sample in difference, and drafting " concentration-voltage " curve simulates straight-line equation from its linear relationship, obtains K, two coefficients of a.
Under the less situation of tracer concentration, " concentration-voltage " is linear.Can obtain the linear equation of " concentration-voltage " according to DS.
The concrete steps of the said method of present embodiment are following, and the described method of this step is the demarcation to a probe:
The step of preparation volumetric solution: be used to prepare the tracer agent that is no less than 10 kinds of variable concentrations and demarcate sample;
Obtain the step of series analog voltage parameter: be used to obtain each probe the variable concentrations tracer agent is demarcated the series analog voltage parameter that sample is exported,, obtain a series of voltage parameter values promptly with respect to a series of known concentration values;
Draw the step of " concentration-voltage " curve map: be used to utilize a series of concentration known values and corresponding a series of voltage parameter values obtained, draw out " concentration-voltage " curve;
Obtain the step of " concentration-voltage " linear equation: be used for according to " concentration-voltage " curve fitting " concentration-voltage " linear equation, fitting precision R is not less than 0.99.And then the characterisitic parameter of acquisition " concentration-voltage " linear equation, i.e. slope K and pitch a.
What should explain at last is; Below only unrestricted in order to technical scheme of the present invention to be described; Although the present invention is specified with reference to the preferred arrangement scheme; Those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention (such as the connected mode of total system, the ordinal relation of each step etc.), and do not break away from the spirit and the scope of technical scheme of the present invention.
Claims (10)
1. the hyperchannel fluorometric investigation system of a waters concentration field; It is characterized in that; Comprise: the tracer agent of in the waters, throwing in; Be arranged on the probe crowd that each sensing point is made up of a plurality of probes in the waters, described probe is connected with vasculum through shielded cable, described vasculum through computer bus or communication network with collection be installed be connected with the central observing and controlling computing machine of calibration software; Described probe comprises the LED pipe that excites said tracer agent to produce fluorescence, receives the photoelectric cell of said fluorescence.
2. test macro according to claim 1; It is characterized in that; Be provided with a power module and a plurality of acquisition module in the described vasculum; Described acquisition module contains the I/V converter that is connected with probe, and described I/V converter selects a switch, A/D converter and communication interface to be connected with eight successively.
3. test macro according to claim 1 and 2 is characterized in that described probe comprises: box body; Said LED pipe and said photoelectric cell are installed in the described box body; Said photoelectric cell advances the light side and is provided with filter set, and described filter set is advanced the light side and is provided with reception light fibre bundle, and described LED pipe bright dipping side is provided with exciting light optical fiber bundle; Described reception light fibre bundle and exciting light optical fiber bundle place the probe rod tube, and the probe rod pipe end is connected with isolating cap; Described LED pipe is electrically connected with modulator and constant current source, and said constant current source is electrically connected with the power module of vasculum; Described photoelectric cell is electrically connected with AC amplifier, detuner, direct current amplifier successively, and the output of described direct current amplifier is electrically connected with I/V converter in the vasculum.
4. test macro according to claim 3; It is characterized in that; Described isolating cap is provided with the hatband that is fixed in probe rod tube one end, and described hatband is divided equally fixing three isolating cap poles by circumference, and the other end of each isolating cap pole is promptly fixed with spacer; Described spacer is the dark circles conical surface of 120 ° of drift angles, about 1cm between described hatband and the spacer
3The space is a measurement zone.
5. test macro according to claim 4 is characterized in that described tracer agent is a uranin.
6. test macro according to claim 5 is characterized in that, the luminescent center wavelength of described LED pipe is the blue light of 488 nanometers; Described photoelectric cell is responsive to 520 nanometer green-yellow lights, and described filter set can be through the light of 510-525 nanometers.
7. method of using the said system of claim 6 to carry out the hyperchannel fluorometric investigation of waters concentration field is characterized in that the step of said method is following:
Throw in the step of tracer agent: be used for the release position selected, throw in the tracer agent of preset concentration in tested waters;
The flow through step of probe of tracer agent: the current that are used to the to carry tracer agent measurement zone of each probe of flowing through;
Detect the step of tracer agent in the current: the current of the tracer-containing of the measurement zone that is used to flow through are by the irradiation of LED pipe; Excite generation fluorescence; Photoelectric cell in the probe converts said fluorescence into the analog quantity current signal and transfers to vasculum, and I/V and A/D conversion via vasculum become the digital voltage parameter;
The step of transmission: be used for vasculum the digital voltage parameter is transferred to central control computer through computer bus or communication network;
The step of circle collection data: be used for each probe of central observing and controlling computing machine scan round, gather the digital voltage parameter that stores each probe;
Calculate the step of tracer concentration: be used for the characterisitic parameter of central observing and controlling computing machine, go out the tracer concentration value of respectively popping one's head in measured by the measured digital voltage calculation of parameter of each probe according to " concentration-voltage " linear equation of each probe configuration file;
Below two steps run parallel:
The step that shows: be used at central observing and controlling computer screen; The interface that shows institute waters of surveying and measuring point thereof layout simulation drawing, the current tracer concentration value of respectively popping one's head in measured of demonstration and on each probe positions with the tracer concentration value symbol of gray scale visual representation;
The step that stores: behind the circle collection, will work as the tracer concentration value storage that time each of collection popped one's head in and surveyed, and fill in the data form.
8. method according to claim 7 is characterized in that, the substep in the described detection current in the step of tracer agent is following:
The substep that light source is modulated: be used for the constant current source of LED pipe power supply is carried out frequency modulation (PFM), make the LED pipe send the exciting light of modulating frequency;
The substep of irradiation tested water body: be used for LED and manage the light that sends has tracer agent through fiber-optic illuminated measurement zone tested water body;
The be excited substep of emitting fluorescence of tracer agent: be used for the illuminated optical excitation of tested water body tracer agent, send fluorescence;
Receive the substep of fluorescence: be used for fluorescence that tracer agent sends through optical fiber, and see through filter set filtering heterochromatic light and reach photoelectric cell, photoelectric cell produces analog current signal;
Amplify and the rectification substep: be used for converting the analog current signal that photoelectric cell produces into analog voltage signal through I/V, rectification after exchanging amplification, the interference of removing heterochromatic light is carried out DC voltage again and is amplified;
The substep of digitized processing: be used for the analog voltage signal after amplifying through DC voltage through the digital voltage signal of A/D conversion process for suitable computer bus transmission or communication network transmission.
9. method according to claim 8 is characterized in that, the modulating frequency described in the substep that the described light source that LED is managed is modulated is 20-28 hertz.
10. the method for the demarcation of the said test macro of claim 6 probe is characterized in that the step of said method is following, and the described method of this step is the demarcation to single probe:
The step of preparation volumetric solution: be used to prepare the tracer agent that is no less than 10 kinds of variable concentrations and demarcate sample;
Obtain the step of series analog voltage parameter: be used to obtain each probe the variable concentrations tracer agent is demarcated the series analog voltage parameter that sample is exported,, obtain a series of voltage parameter values promptly with respect to a series of known concentration values;
Draw the step of " concentration-voltage " curve map: be used to utilize a series of concentration known values and corresponding a series of voltage parameter values obtained, draw out " concentration-voltage " curve;
Obtain the step of " concentration-voltage " linear equation: be used for going out " concentration-voltage " linear equation, and then obtain the characteristic parameter of equation: slope K and pitch a according to " concentration-voltage " curve fitting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104084835A CN102519927B (en) | 2011-12-09 | 2011-12-09 | Multichannel fluorescence test system and method of water concentration field |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104084835A CN102519927B (en) | 2011-12-09 | 2011-12-09 | Multichannel fluorescence test system and method of water concentration field |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102519927A true CN102519927A (en) | 2012-06-27 |
| CN102519927B CN102519927B (en) | 2013-12-11 |
Family
ID=46290920
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104084835A Active CN102519927B (en) | 2011-12-09 | 2011-12-09 | Multichannel fluorescence test system and method of water concentration field |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102519927B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104006946A (en) * | 2014-06-10 | 2014-08-27 | 珠江水利委员会珠江水利科学研究院 | Layering tracing method for river mouth salt tide salt-fresh water density stratified flows |
| CN104614346A (en) * | 2013-11-05 | 2015-05-13 | 上海阔思电子有限公司 | Circulating water agent concentration detector |
| CN105021587A (en) * | 2015-08-28 | 2015-11-04 | 上海清淼光电科技有限公司 | Multi-chain hydrocarbon substance measuring device and method based on ultraviolet fluorescence method |
| CN106596495A (en) * | 2016-12-29 | 2017-04-26 | 国家纳米科学中心 | Multichannel fluorescence correlation spectroscopy-based multimer detection data processing method |
| CN107014775A (en) * | 2017-03-02 | 2017-08-04 | 珠江水利委员会珠江水利科学研究院 | Based on optical water body concentration field measurement method |
| CN110749642A (en) * | 2019-10-29 | 2020-02-04 | 中广核工程有限公司 | Tracer element concentration detection method for steam generator humidity measurement test |
| CN111220785A (en) * | 2020-01-21 | 2020-06-02 | 安徽省（水利部淮河水利委员会）水利科学研究院（安徽省水利工程质量检测中心站） | Method for detecting water age of water body |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06289008A (en) * | 1993-02-08 | 1994-10-18 | Nalco Chem Co | Method for monitoring water characteristic in treating process of raw water or waste water |
| CN1113569A (en) * | 1994-02-14 | 1995-12-20 | 诺尔科化学公司 | Regulating water treatment agent dosage based on operational system stresses |
| CN1308232A (en) * | 2001-01-19 | 2001-08-15 | 丁杰 | Tracer matter method of detecting water treating chemical content in industrial circulation water |
| CN2793729Y (en) * | 2005-02-02 | 2006-07-05 | 上海东方机电科技公司 | Long-range on-line analyzing inspector for electric conductivity, pH value and oxygen dissolving value |
| JP3920211B2 (en) * | 2002-12-24 | 2007-05-30 | 株式会社東芝 | Elemental analysis method and apparatus |
| KR100809280B1 (en) * | 2006-10-24 | 2008-03-03 | 주식회사 포스코 | Effluents tracing method |
| CN101184987A (en) * | 2005-05-02 | 2008-05-21 | 纳尔科公司 | Method for using an all solid-state fluorometer in monitoring and controlling chemicals in water |
| CN101464471A (en) * | 2009-01-15 | 2009-06-24 | 清华大学 | Real-time measurement system for solid model surface flow field and its reprocessing method |
| CN101943694A (en) * | 2009-07-08 | 2011-01-12 | 上海衡伟信息技术有限公司 | Water quality on-line monitoring system |
| JP2011145186A (en) * | 2010-01-15 | 2011-07-28 | Mitsubishi Heavy Ind Ltd | Radioactive element detecting compound, radioactive element detecting instrument using the same, and radioactive element detection method |
-
2011
- 2011-12-09 CN CN2011104084835A patent/CN102519927B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06289008A (en) * | 1993-02-08 | 1994-10-18 | Nalco Chem Co | Method for monitoring water characteristic in treating process of raw water or waste water |
| CN1113569A (en) * | 1994-02-14 | 1995-12-20 | 诺尔科化学公司 | Regulating water treatment agent dosage based on operational system stresses |
| CN1308232A (en) * | 2001-01-19 | 2001-08-15 | 丁杰 | Tracer matter method of detecting water treating chemical content in industrial circulation water |
| JP3920211B2 (en) * | 2002-12-24 | 2007-05-30 | 株式会社東芝 | Elemental analysis method and apparatus |
| CN2793729Y (en) * | 2005-02-02 | 2006-07-05 | 上海东方机电科技公司 | Long-range on-line analyzing inspector for electric conductivity, pH value and oxygen dissolving value |
| CN101184987A (en) * | 2005-05-02 | 2008-05-21 | 纳尔科公司 | Method for using an all solid-state fluorometer in monitoring and controlling chemicals in water |
| KR100809280B1 (en) * | 2006-10-24 | 2008-03-03 | 주식회사 포스코 | Effluents tracing method |
| CN101464471A (en) * | 2009-01-15 | 2009-06-24 | 清华大学 | Real-time measurement system for solid model surface flow field and its reprocessing method |
| CN101943694A (en) * | 2009-07-08 | 2011-01-12 | 上海衡伟信息技术有限公司 | Water quality on-line monitoring system |
| JP2011145186A (en) * | 2010-01-15 | 2011-07-28 | Mitsubishi Heavy Ind Ltd | Radioactive element detecting compound, radioactive element detecting instrument using the same, and radioactive element detection method |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614346A (en) * | 2013-11-05 | 2015-05-13 | 上海阔思电子有限公司 | Circulating water agent concentration detector |
| CN104614346B (en) * | 2013-11-05 | 2018-06-01 | 上海阔思电子有限公司 | Recirculated water drug concentration detector |
| CN104006946A (en) * | 2014-06-10 | 2014-08-27 | 珠江水利委员会珠江水利科学研究院 | Layering tracing method for river mouth salt tide salt-fresh water density stratified flows |
| CN105021587A (en) * | 2015-08-28 | 2015-11-04 | 上海清淼光电科技有限公司 | Multi-chain hydrocarbon substance measuring device and method based on ultraviolet fluorescence method |
| CN106596495A (en) * | 2016-12-29 | 2017-04-26 | 国家纳米科学中心 | Multichannel fluorescence correlation spectroscopy-based multimer detection data processing method |
| CN106596495B (en) * | 2016-12-29 | 2019-04-02 | 国家纳米科学中心 | A kind of polymer detection data processing method of multichannel fluorescence correlation spectroscopy |
| CN107014775A (en) * | 2017-03-02 | 2017-08-04 | 珠江水利委员会珠江水利科学研究院 | Based on optical water body concentration field measurement method |
| CN107014775B (en) * | 2017-03-02 | 2019-09-24 | 珠江水利委员会珠江水利科学研究院 | Based on optical water body concentration field measurement method |
| CN110749642A (en) * | 2019-10-29 | 2020-02-04 | 中广核工程有限公司 | Tracer element concentration detection method for steam generator humidity measurement test |
| CN111220785A (en) * | 2020-01-21 | 2020-06-02 | 安徽省（水利部淮河水利委员会）水利科学研究院（安徽省水利工程质量检测中心站） | Method for detecting water age of water body |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102519927B (en) | 2013-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102519927B (en) | Multichannel fluorescence test system and method of water concentration field | |
| CN100476389C (en) | Luminous flux measurement device using standard light source in narrow beam for LED, and testing method | |
| CN100378449C (en) | Instrument and method for testing fluid characteristics | |
| CN101387605B (en) | Optical fiber agricultural land soil organic matter content rapid detector | |
| CN104777108B (en) | The detection means and method of a kind of chlorophyll content | |
| CN102279174B (en) | Alga identification and measurement sensor and method | |
| CN101975764B (en) | Near infrared spectrum technology-based multiband soil nitrogen detection device and method | |
| CN103323400A (en) | Multi-parameter integrated water quality on-line monitoring sensing system | |
| CN111089852A (en) | Vertical section distribution in-situ detection system for petroleum hydrocarbon pollutants in polluted site | |
| CN104155247A (en) | In-situ measurement method and device for chlorophylls and turbidity of water body | |
| CN103528991B (en) | System and method for measuring organic matter content of soil | |
| CN113310665A (en) | Quality detection device for operation shadowless lamp | |
| CN106442428A (en) | Optical fiber type measurement method based on visibility of multiple times of reflection | |
| CN113984700A (en) | Non-contact near-infrared soil moisture content online detection device and method | |
| CN204287043U (en) | Based on soil heavy metal cadmium and the copper content detection device of induced with laser spectral technique | |
| CN205958442U (en) | Double -light -path water environment on - line measuring device based on spectral measurement technique | |
| Fan et al. | An Optical Soil Sensor for NPK Nutrient Detection in Smart Cities | |
| CN103149180B (en) | Detection method of soil spectral reflectivity and specific conductance | |
| CN102221391B (en) | Light controlling device for measuring penetrant level of soil sample | |
| CN103097877A (en) | Portable reflectometer and method for characterising the mirrors of solar thermal power plants | |
| CN103558177B (en) | Raw meat organizes moisture near-infrared detection apparatus and detection method | |
| CN108333085B (en) | Photoelectric vertical line sand content detection method with capacitance and color compensation | |
| CN203705343U (en) | Oceanic chlorophyll fluorescence in situ monitor | |
| CN107764771A (en) | Visual imaging detection device for water microorganisms | |
| CN201788146U (en) | Pulsed xenon lamp type soil nutrient tester |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |