CN102499243A - Biological disinfectant - Google Patents
Biological disinfectant Download PDFInfo
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- CN102499243A CN102499243A CN2011103309668A CN201110330966A CN102499243A CN 102499243 A CN102499243 A CN 102499243A CN 2011103309668 A CN2011103309668 A CN 2011103309668A CN 201110330966 A CN201110330966 A CN 201110330966A CN 102499243 A CN102499243 A CN 102499243A
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- Prior art keywords
- disinfectant
- biological disinfectant
- biological
- polylysine
- epsilon
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a biological disinfectant with safety, high efficiency and low irritation. One milliliter of the biological disinfectant comprises the following components: 0.5 to 10 milligrams of epsilon-polylysine, 1 to 20 milligrams of glycerol, 0.8 to 16 milligrams of dodecyl dimethyl betaine and the balance of buffer solution with the pH value of 6.0 t 7.5. The biological disinfectant can be used for disinfecting hand skin, the surfaces of objects and the like. The sterilizing log value of the biological disinfectant on Escherichia coli and candida albicans within 3 minutes is more than 3. The sterilizing log value of the biological disinfectant on pseudomonas aeruginosa and staphylococcus aureus is more than 6. As the contact lasts, a disinfecting effect becomes better.
Description
Technical field
The present invention relates to disinfectant preparation and applied technical field thereof, a kind of biological antiseptic preparation that contains epsilon-polylysine especially is provided.
Background technology
The disinfectant kind is main with chemosterilant in the market; The effective ingredient of nearly all disinfectant concentrates on following several kinds: the compound disinfectant of chlorhexidine acetate, iodine, povidone-iodine, hibitane, BZK, polyhexamethylene guanide, dialkyl quaternary ammonium salt and chlorhexidine acetate mixture, single double-chain quaternary ammonium salt, multiple alcohol preparation (ethanol, isopropyl alcohol, ethylene glycol, butanediol and have the high molecular alcohol etc. of utmost point group, total pure content 55%), compound disinfectant (650 mg/L low-concentration hcl poly hexamethylene biguanides carry out compatibility with two decyl alkyl dimethyl ammonium chlorides, benzalkonium bromide, ethanol, chlorhexidine acetate respectively), Chinese medical extract (Osthole, menthol, matrine, rheum officinale, turmeric, Huang Cen, Radix Glycyrrhizae and menthol etc.) etc.Chemosterilant is wanted strict control dosage in use, and majority had excitant when concentration was high, and can cause corrosion to skin or equipment etc., and this brings very big inconvenience for actual the use.
Desirable disinfectant should have wide spectrum, antibacterial activity efficiently, to human body and environment have no side effect, nonirritant; Will be with sterilization surperficial compatible, and easy to use, stable performance, cheap, no any offending smell.The research direction that has at present obtained remarkable break-throughs and had practical potentiality has biological enzyme, peroxide disinfectant, nano-metal-oxide and the coating or the like of sterilizing automatically.Particularly biology enzyme and automatic sterilization coating are listed in decontamination modernization strategic plan at the beginning of its 21 century by U.S. Department of Defense.Domestic researcher is also in the development of constantly being devoted to biological disinfectant, three patent reports have been arranged the at present development of biological disinfectant: the development of the disinfectant of a kind of biological disinfectant, compound lysostaphin enzyme disinfectant and antibacterial peptide GK that contains Wood frog antibiotic peptides.Relevant therewith product is a compound lysostaphin enzyme disinfectant and Ci Hua board marine organisms plain thimerosal alive.Because antimicrobial component is safe, so biological disinfectant has higher safety.
Summary of the invention
The object of the present invention is to provide a kind of safe, efficient, biological disinfectant that excitant is low.
Biological disinfectant of the present invention comprises following component: epsilon-polylysine 0.5～10 mg/mL, glycerine: 1～20 mg/mL, dodecyldimethylammonium hydroxide inner salt: 0.8～16 mg/mL, surplus is the buffer solution of pH value 6.0-7.5.
Biological disinfectant of the present invention also comprises component glycine: 1mg/mL～10 mg/mL or cocamidopropyl betaine: 1～20 mg/mL.
(ε-polylysine) is by the synthetic a kind of polypeptide that is formed through ε-amido link by the lysine monomer of microorganism streptomyces albus fermentation to epsilon-polylysine; Can directly be adsorbed onto on the cell membrane; Finally cause cell death, perhaps suppress the respiration of bacterium, act on cell membrane and protein-synthesizing system simultaneously; Combine the synthetic of CKIs and enzyme with ribosome, thereby reach the sterilization purpose.Epsilon-polylysine can be decomposed into lysine in human body, not only have no toxic and side effect, and can be used as a kind of source of lysine.Therefore, epsilon-polylysine belongs to the natural bacteriostatic agent, is that main component researches and produces disinfectants and can give the characteristics that disinfectant is safe and efficient, excitant is low with it.The present invention can be used for the sterilization of hand skin, body surface etc.; Its in 3min to the sterilization logarithm value of Escherichia coli and Candida albicans greater than 3; Greater than 6, and along with the prolongation of time of contact, its Disinfection Effect is better to the sterilization logarithm value of Pseudomonas aeruginosa and staphylococcus aureus.Carry out long-lasting experiment according to disinfection technology standard; Experimental result is illustrated in 37 ℃ and placed 3 months; The Disinfection Effect of this biological disinfectant does not almost change, and this this biological disinfectant of explanation is relatively stable, at room temperature places can keep original Disinfection Effect basically in 2 years.
Embodiment
Below in conjunction with specific embodiment the present invention is further specified.
Embodiment 1:
Biological disinfectant contains following component: epsilon-polylysine 5 mg/mL, and glycine 5 mg/mL, dodecyldimethylammonium hydroxide inner salt (BS-12) 8 mg/mL, glycerine 2 mg/mL, surplus is the buffer solution of pH value 7.0.
The preparation method is following:
Take by weighing epsilon-polylysine (purity>90%) 556 mg; Glycine 500 mg; 30% dodecyldimethylammonium hydroxide inner salt (BS-12), 800 mg; Glycerine 200 mg join it in (pH value=7.0) buffer solution that contains 0.2 M sodium hydrogen phosphate and 0.1 M citric acid, and making its cumulative volume is 100 mL.
Embodiment 2:
Biological disinfectant contains following component: epsilon-polylysine 0.5 mg/mL; Dodecyldimethylammonium hydroxide inner salt (BS-12) 2 mg/mL; Cocamidopropyl betaine (CA-35) 10 mg/mL, glycerine 1 mg/mL, surplus is the buffer solution of pH value 7.0.
The preparation method is following:
Take by weighing epsilon-polylysine (purity>90%) 56 mg; 30% dodecyldimethylammonium hydroxide inner salt (BS-12), 200 mg; 30% cocamidopropyl betaine (CA-35), 1000 mg; Glycerine 100 mg join it in (pH value=6.8) buffer solution that contains 0.2 M sodium hydrogen phosphate and 0.1 M citric acid, and making its cumulative volume is 100 mL.
Embodiment 3:
Biological disinfectant contains following component: epsilon-polylysine 2 mg/mL, and dodecyldimethylammonium hydroxide inner salt (BS-12) 16 mg/mL, glycerine 4 mg/mL, surplus is the buffer solution of pH value 7.2.
The preparation method is following:
Take by weighing epsilon-polylysine (purity>90%) 222 mg; 30% dodecyldimethylammonium hydroxide inner salt (BS-12), 1600 mg; Glycerine 400 mg; Join it in (pH value=7.2) buffer solution that contains 0.2 M sodium hydrogen phosphate and 0.1 M citric acid, making its cumulative volume is 100 mL.
Embodiment 4:
Biological disinfectant contains following component: epsilon-polylysine 8 mg/mL, and dodecyldimethylammonium hydroxide inner salt (BS-12) 12 mg/mL, glycerine 15 mg/mL, surplus is the buffer solution of pH value 7.2.
The application of biological disinfectant:
The biological disinfectant that adopts present technique to produce can be applied to the sterilization of daily hand skin and article surface etc.It has good killing action to skin and common gram-positive bacteria and the negative bacterium of article surface.
Disinfection experiment:
Disinfectant microbicidel experiment according to regulation in " disinfection technology standard " is carried out, and used bacterial strain is Escherichia coli (8099), staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 9072), Candida albicans (ATCC 10231).
Stability experiment:
According to the carry out disinfection long-lasting experiment of agent of the stability experiment of " disinfection technology standard ", be about to disinfectant and placed 3 months in 37 ℃.Experiment then carries out disinfection.
Experimental result: the Disinfection Effect of table 1 biological disinfectant
Annotate: a is freshly prepd disinfectant, and b is the disinfectant of placement after 3 months.
Claims (3)
1. a biological disinfectant is characterized in that, comprises following component: epsilon-polylysine 0.5～10 mg/mL, glycerine: 1～20 mg/mL, dodecyldimethylammonium hydroxide inner salt: 0.8～16 mg/mL, surplus is the buffer solution of pH value 6.0-7.5.
2. biological disinfectant according to claim 1 is characterized in that, comprises glycine: 1mg/mL～10 mg/mL.
3. biological disinfectant according to claim 1 is characterized in that, comprises cocamidopropyl betaine: 1～20 mg/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103309668A CN102499243A (en) | 2011-10-27 | 2011-10-27 | Biological disinfectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103309668A CN102499243A (en) | 2011-10-27 | 2011-10-27 | Biological disinfectant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102499243A true CN102499243A (en) | 2012-06-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103309668A Pending CN102499243A (en) | 2011-10-27 | 2011-10-27 | Biological disinfectant |
Country Status (1)
| Country | Link |
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| CN (1) | CN102499243A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104644667A (en) * | 2015-02-12 | 2015-05-27 | 天津大学 | Compound disinfectant and preparation method thereof |
| CN105507014A (en) * | 2015-12-07 | 2016-04-20 | 宋宏婷 | Composite fiber fabric |
| WO2016070046A1 (en) * | 2014-10-30 | 2016-05-06 | Karen Troxel | Methods of preventing surgical site infections |
| CN106943425A (en) * | 2017-03-01 | 2017-07-14 | 北京德得创业科技有限公司 | A kind of gynaecology sterilization gel of wound healing and preparation method and application |
| CN110903702A (en) * | 2019-12-06 | 2020-03-24 | 云南凤凰纸制品有限公司 | Antibacterial agent suitable for tipping paper |
| CN111202069A (en) * | 2020-02-13 | 2020-05-29 | 军事科学院系统工程研究院卫勤保障技术研究所 | Disinfectant, and preparation method and application thereof |
| CN112352796A (en) * | 2020-11-27 | 2021-02-12 | 四川奥洁科技有限公司 | Special disinfection coupling gel for medical ultrasound and preparation method thereof |
| CN115778849A (en) * | 2022-12-19 | 2023-03-14 | 江苏百赛飞生物科技有限公司 | Antibacterial wet tissue capable of being used for cleaning hands and mouths of infants |
Citations (2)
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| US20040228826A1 (en) * | 2003-05-15 | 2004-11-18 | Yuichi Yamamoto | Silicone-modified antimicrobial polymer, antimicrobial agent and antimicrobial resin composition |
| CN101658705A (en) * | 2009-04-23 | 2010-03-03 | 陈旭良 | Medical humidification fluid with bacteria resistance function |
-
2011
- 2011-10-27 CN CN2011103309668A patent/CN102499243A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040228826A1 (en) * | 2003-05-15 | 2004-11-18 | Yuichi Yamamoto | Silicone-modified antimicrobial polymer, antimicrobial agent and antimicrobial resin composition |
| CN101658705A (en) * | 2009-04-23 | 2010-03-03 | 陈旭良 | Medical humidification fluid with bacteria resistance function |
Non-Patent Citations (4)
| Title |
|---|
| 《氨基酸和生物资源》 20050630 张东荣等 "聚赖氨酸的研究进展" 第48-51 1-3 第27卷, 第2期 * |
| 《食品与发酵工业》 20050630 施庆珊等 "epsilon-聚赖氨酸的抑菌特性及其应用前景" 第76-79页 1-3 第31卷, 第6期 * |
| 张东荣等: ""聚赖氨酸的研究进展"", 《氨基酸和生物资源》 * |
| 施庆珊等: ""ε-聚赖氨酸的抑菌特性及其应用前景"", 《食品与发酵工业》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016070046A1 (en) * | 2014-10-30 | 2016-05-06 | Karen Troxel | Methods of preventing surgical site infections |
| AU2015338969B2 (en) * | 2014-10-30 | 2020-08-27 | Biomet Manufacturing, Llc | Methods of preventing surgical site infections |
| CN104644667A (en) * | 2015-02-12 | 2015-05-27 | 天津大学 | Compound disinfectant and preparation method thereof |
| CN105507014A (en) * | 2015-12-07 | 2016-04-20 | 宋宏婷 | Composite fiber fabric |
| CN106943425A (en) * | 2017-03-01 | 2017-07-14 | 北京德得创业科技有限公司 | A kind of gynaecology sterilization gel of wound healing and preparation method and application |
| CN110903702A (en) * | 2019-12-06 | 2020-03-24 | 云南凤凰纸制品有限公司 | Antibacterial agent suitable for tipping paper |
| CN110903702B (en) * | 2019-12-06 | 2022-03-15 | 云南凤凰纸制品有限公司 | Antibacterial agent suitable for tipping paper |
| CN111202069A (en) * | 2020-02-13 | 2020-05-29 | 军事科学院系统工程研究院卫勤保障技术研究所 | Disinfectant, and preparation method and application thereof |
| CN112352796A (en) * | 2020-11-27 | 2021-02-12 | 四川奥洁科技有限公司 | Special disinfection coupling gel for medical ultrasound and preparation method thereof |
| CN115778849A (en) * | 2022-12-19 | 2023-03-14 | 江苏百赛飞生物科技有限公司 | Antibacterial wet tissue capable of being used for cleaning hands and mouths of infants |
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Application publication date: 20120620 |