CN102492608A - Illumination system for continuously culturing microalgae and illumination method thereof - Google Patents
Illumination system for continuously culturing microalgae and illumination method thereof Download PDFInfo
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- CN102492608A CN102492608A CN201110406198XA CN201110406198A CN102492608A CN 102492608 A CN102492608 A CN 102492608A CN 201110406198X A CN201110406198X A CN 201110406198XA CN 201110406198 A CN201110406198 A CN 201110406198A CN 102492608 A CN102492608 A CN 102492608A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
Abstract
The invention provides an illumination system for continuously culturing microalgae. The illumination system comprises a box-shaped lamp holder connected with an electric control cabinet, illumination lamp tubes horizontally arranged in the lamp holder frame and a switch used for controlling the powered-on or powered-off of the illumination lamp in the electric control cabinet, wherein wires connected with the electric control cabinet are distributed inside the lamp holder, and the two ends of the illumination lamp tubes are connected with the lamp holder by virtue of plugs at the tail ends of the wires. The illumination system and bagging type optic bioreactors which are suspended on a bracket are arranged in parallel; through controlling the switch in the electric control cabinet, the quantity of the illumination lamp tubes in the lamp holder is controlled, or the illumination lamp tubes with different power are selected, so as to achieve the illumination strengths meeting different demands from the inoculation of the microalgae to the harvesting of the microalgae. The establishment of the three-dimensional vertically arranged illumination system which is parallel to the reactors provides sufficient illumination for the microalgae in the reactor. The illumination system is vertically arranged on the ground, so that illumination is provided for the vertical reactors arranged at the two sides of the illumination system, thereby greatly and sufficiently utilizing space and illumination and saving electric power.
Description
Technical field
The present invention relates to a kind of illumination system and illumination method thereof of unit cell algae cultured continuously, when being applicable to unit cell algae continuous culture system cultivation unit cell algae, is that reaction system provides illumination as artificial light.
Background technology
In the process of cultivating the unit cell algae, illumination is a crucial factor, and the growth and breeding of unit cell algae is all had very significant effects.In culturing process, if can not obtain competent illumination, the growth of unit cell algae possibly stagnated even be dead.Therefore, no matter be laboratory culture or expansion kind of cultivation or productivity cultivation to the unit cell algae, all effective illumination need be provided, when lamp was difficult to satisfy the growth of unit cell algae, artificial light just became new selection.At present, in the culturing process of unit cell algae, the factor of required illumination when all artificial light having been grown as the unit cell algae.During like laboratory culture, except obtaining through lamp the illumination, need be that the growth and breeding of unit cell algae provides illumination as artificial light also with fluorescent lamp etc.Expand when kind cultivating with the productivity cultivation, also all need come as artificial light with fluorescent lamp etc. is that the growth and breeding of unit cell algae provides illumination.This kind illumination generally all is culture vessel or the water body top that is suspended on top ceiling or needs illumination.
The weak point that prior art exists is; When no matter being laboratory culture or expansion kind of cultivation or productivity cultivation, when cultivating on a small quantity, lamp just can be satisfied the demand; But when culture density is big; Particularly to the cultivation of unit cell algae during requiring on free, when the growth cycle that promptly will shorten the unit cell algae need provide the illumination at night, will adopt artificial light such as fluorescent lamp to throw light on for a long time.But this illumination is simple, coarse, only need place culture vessel or cultivates the water body top and carry out illumination and get final product.The time length of illumination when needs unit cell algae faster growing, when the density of the unit cell algae of perhaps cultivating is big, is carried out more irradiation just through carrying out after the artificial guestimate, otherwise, shine on a small quantity or do not shine.Illumination is difficult to water body that see through to cultivate, particularly along with the amplification of the scale of cultivation, kind produces or productivity when cultivating expanding, and the increase of water body volume, the increasing of the depth of water, the illumination of general fluorescent tube is difficult to penetrate water body.Will cause the situation that the unit cell algae growth of distance light end is bad even stagnate.When serious, can cause the realization that is difficult to of unit cell algae cultivation.
Insufficient reason is more than the existence, and the employing of at first this illumination mode is to adapt with relevant unit cell algae training method, is one.Unit cell algae best cultivation has determined the simple still complicated, coarse or meticulous of supporting with it illumination mode.And existing unit cell algae training method, during like laboratory culture, the quantity of the unit cell algae of cultivation is few and environment that cultivate is better, and general illumination promptly can be satisfied the requirement of unit cell algae growth and breeding.Simple fluorescent lamp is radiated at when illumination is provided, and just can satisfy the requirement of unit cell algae to illumination.Therefore, do not have improved necessity yet.Expand and plant when cultivating, employing barrel cage etc. is cultivated, and general fluorescent lamp provides illumination, just can satisfy the requirement of unit cell algae to illumination.Do not carry out complicated design yet and perhaps elaborate necessity of an illumination system.Requirement to illumination system does not have improved power.And when productivity is cultivated; The existing cultivation of carrying out the unit cell algae with glass reinforced plastic tank, cement pit or Tu Chi etc.; The water body of cultivating is big, and the depth of water is darker, but the density of the unit cell algae of cultivating is little; Training method is comparatively extensive, though arrange above water body that therefore some fluorescent tubes provide illumination and illumination also can make the unit cell algae cultivate out.But there has been a lot of deficiencies in this mode; Though as place the fluorescent tube of water body top can be according to the requirement of illumination; Through what selection obtains the illumination of suitable size to fluorescent tube, but the light that fluorescent tube exhales can't shine whole cultivation water body, can be unfavorable to the growth of the unit cell algae of water bottom; The unit cell algae of bottom can't obtain effective illumination even not have illumination, can influence its growth.Also make existing productivity cultivate the production that can't realize a large amount of intensifications.On the one hand, existing productivity training method has restricted the progress of illumination system, and on the other hand, too simple extensive scale and the technology that also makes productivity cultivate of existing illumination system can't improve.
Yet in the existing lighting system, the simple illumination of fluorescent lamp can only satisfy existing training method substantially.The existing mode of production has restricted the performance of the effect of lighting systems such as fluorescent lamp, and the illumination of fluorescent lamp can't see through whole water body.In case after emerging unit cell algae continuous culture system and working method were set up, simple fluorescent lamp lighting just can not satisfy.
After emerging unit cell algae continuous culture system and method are set up, design the reactor drum made as a kind of vertical cultivation unit cell algae container, need good illumination could realize the production that intensifies.The illumination system that adapts with it is badly in need of setting up, and needs characteristics and the growth phase according to the unit cell algae of being cultivated, and obtains corresponding intensity of illumination.
Summary of the invention
The illumination system and the illumination method thereof that the purpose of this invention is to provide a kind of unit cell algae cultured continuously provide illumination in the time of can being cultured continuously unit cell algae as artificial light, to overcome the deficiency of no special illumination system and method in the prior art.
Technical conceive of the present invention is following:
The foundation of unit cell algae continuous culture system perhaps frees the culture vessel of cultivation on conventional floor of unit cell algae the underground cultivation water body, form the culture systems that a kind of spatial efficiently utilizes the useful space.The foundation of this system also needs a cover and the closely-related illumination system of this system as the illumination support except the corresponding support system support of needs.
Be difficult to penetrate whole water layer in order to solve the last illumination of production; The not high problem of production efficiency that makes the cultivation of unit cell algae; The particularly unit cell algae cessation of growth cessation of distance light end; And the whole production efficient that causes is not high, is difficult to realize the problem of effectively producing, should make illumination object that institute designs making particularly fluorescent lamp to shine with fluorescent lamp over against water body; Therefore the degree of depth of the relative fluorescent lamp of water body should not crossed dark or not want long widely, must make that fluorescent lamp can be according to thoroughly and the straight water layer of ray.
This method is body isochronous surfac degree of facing danger or disaster in the pond, but after unit cell algae continuous culture system is set up, particularly after the hanging-bag type bioreactor is set up; The fluorescent lamp that only need design and produce out a parallel end with reactor drum gets final product; For the characteristics with vertical higher reactor drum adapt, also need a plurality of fluorescent tubes of vertical array, simultaneously; In order to make full use of the light that vertically disposed fluorescent tube emits, can design again at the opposite side of fluorescent tube and erect row's reactor drum.
Come for fluorescent tube is holded up, and arrange, need design and produce a lamp bracket and make it to erect and come and parallel with it with the reactor drum parallel longitudinal.
Be fixed on the situation such as electric leakage that ground possibly caused for a long time for fear of lamp bracket, need a lamp bracket of design and higher support is arranged.
In order to prevent the corrosion of seawater, lamp bracket adopts corrosion resistant metallic substance to make, and on metal, is coated with the last layer corrosion protection coating.
Therefore; Based on current emerging unit cell algae continuous culture system and hanging-bag type bioreactor thereof; The technical scheme of the illumination system of unit cell algae cultured continuously of the present invention is following: this illumination system comprises the lamp bracket of a square frame shape that is connected with power control box; It is characterized in that also comprising the illuminating lamp tube that is horizontally set in the lamp bracket frame, the lamp bracket internal layout has and the power control box wire connecting, and the two ends of illuminating lamp tube are connected with lamp bracket via the plug of wire terminations; The junction of described illuminating lamp tube and plug is provided with the waterproof crown top of burner, the switch that power control box inner control illuminating lamp tube is opened or closed.
Because the unit cell algae is at different growth phases; Needed intensity of illumination is differentiated; In order to satisfy this difference; Suit the needs of the various growth phases of unit cell algae to light better, the number of the fluorescent tube that passes through to be adopted and the power of every fluorescent tube are realized different illumination intensity in each in period.How much bright the lamp of number that fluorescent tube need be bright and which concrete power is bright,, how long bright, how bright, all realizes through power control box.
Because whole culture systems all will contact with seawater and other water body, prevent electric leakage, anti-sealing gets in electric wire and the fluorescent tube lamp holder; The present invention has designed and produced a waterproof crown top of burner; Can make seawater not get in the lamp holder electric wire, the situation that can not occur leaking electricity more can not corroded fluorescent tube; The described waterproof crown top of burner is the columniform cap-like structure body that one one end opens wide, and its other end is provided with flat perforate; The shape and the perforate of described plug xsect are consistent, and are provided with boss with the contacted end of illuminating lamp tube.
Set up 6～10 tubes can satisfy the primary demand of unit cell algae cultured continuously in the above-mentioned lamp bracket from top to bottom.
The spacing of above-mentioned fluorescent tube is in 400～600mm scope, and the length of fluorescent tube can maximally utilise light source and save electric energy in 600～800mm scope.
Because the light source of existing cultivation unit cell algae is a natural light; Do not have the research of what scope intensity of illumination of concrete needs; Yet utilize the present invention, the luminous intensity of above-mentioned illuminating lamp tube promptly can satisfy the demand of cultured continuously unit cell algae in 200～30000lx scope.
The method of utilizing above-mentioned illumination system in unit cell algae cultured continuously, the unit cell algae to be carried out illumination; Comprise the hanging-bag type bioreactor is hung on the support; Again the hanging-bag type bioreactor is connected with inflation system with feed liquid adding system, algae liquid gathering system respectively; And unit cell algae algae kind is inoculated in the hanging-bag type bioreactor, it is characterized in that further comprising the steps of:
Described illumination system and the hanging-bag type bioreactor that hangs on the support are arranged side by side; Through the quantity of illuminating lamp tube in the on-off control lamp bracket in the power control box, or the illuminating lamp tube of selection different capacity, the intensity of illumination of different demands when being implemented in unit cell algae inoculation back to results.Specific as follows: when inoculation, the intensity of illumination in daytime, evening is controlled at 2000～30000lx scope; At lag phase, the intensity of illumination in daytime, evening is controlled at 1000～30000lx scope; In exponential phase of growth, the intensity of illumination in daytime, evening is controlled at 2000～30000lx scope; At the allometry decrement phase, the intensity of illumination on daytime is controlled at 1000～20000lx scope, and the intensity of illumination in evening is controlled at 600～10000lx scope; In stationary phase, the luminous intensity on daytime is controlled at 600～10000lx scope, and the luminous intensity at night is controlled at 200～6000lx scope; When results, need not illumination.
Illumination system of the present invention not only will provide effective illumination source for whole culture systems; The more important thing is structure through the spatial illumination system; Laterally arrange with bioreactor,, add that like this inflatable in the reactor drum stirs for reactor drum provides parallel in-plant illumination at arbitrary height; Unit cell algae is wherein constantly stirred, obtain effective illumination more.Competent illumination source is provided and the illumination that parallel illuminating lamp tube provides is also can low coverage liftoff for the unit cell algae in the reactor drum.The foundation of this illumination system has solved from cultivating the illumination that provides illumination to bring above the main body and has been difficult to penetrate whole culture system, thereby causes the unit cell algae can't obtain effective illumination, is difficult to the problem that realizes that the high-density intensification is cultivated.Be a great technological breakthrough, and the foundation of the illumination system that parallels with reactor drum that spatial is vertically arranged is for the unit cell algae in the reactor drum provide sufficient illumination.Illumination system is through erectting discharging on the ground, and the vertical reactor drum that can be both sides provides illumination, has made full use of illumination widely, has saved electric power, has made full use of existing space.
Description of drawings
Fig. 1 is an illumination system structural representation of the present invention.
Fig. 2 is the structural representation of plug of the present invention.
Fig. 3 is the structure perspective diagram of plug of the present invention.
The structural representation of Fig. 4 waterproof crown top of burner of the present invention.
Wherein, 1, power control box, 2, lamp bracket, 3, illuminating lamp tube, 4, the waterproof crown top of burner, 5, plug, 6, switch, 7, perforate, 8, boss.
Embodiment
Like Fig. 1～shown in Figure 4, can parallel vertical array in order to make illuminating lamp tube 3 with reactor drum, and separate with ground; Designing a lamp bracket 2 sets up illuminating lamp tube 3; To realize control in order making for circuit and illuminating lamp tube 3, also need to design a power control box 1, and both are connected.
The illumination system of unit cell algae cultured continuously of the present invention; The lamp bracket 2 that comprises a square frame shape that is connected with power control box 1; It is characterized in that also comprising the illuminating lamp tube 3 that is horizontally set in lamp bracket 2 frames; Thereby make illuminating lamp tube 3 vertical array, divide many in the space of different heights vertical array, to parallel with reactor drum; For the fluorescent tube making from extraneous backguy to lamp bracket, and avoid circuit more random, stay potential safety hazard; Lamp bracket 2 internal layout have and power control box 1 wire connecting, are fixed on the lamp bracket 2 in order to make illuminating lamp tube 3, and avoid seawater to get into illuminating lamp tube 3 lamp holder places; The danger of electric leakage takes place; Also avoid being corroded, and the two ends of illuminating lamp tube 3 are connected with lamp bracket 2 via the plug 5 of wire terminations, described illuminating lamp tube 3 is provided with the waterproof crown top of burner 4 with the junction of plug 5; In order to realize real-time control, the switch 6 that power control box 1 inner control illuminating lamp tube 3 is opened or closed to illuminating lamp tube 3 on the lamp bracket 2.
In order to make illuminating lamp tube 3 be connected better with electric wire; Also for waterproof better; Avoid taking place security incidents such as electric leakage, also for illuminating lamp tube 3 is contacted with electric wire better, waterproof better; The above-mentioned waterproof crown top of burner 4 is columniform cap-like structure bodies that one one end opens wide, and its other end is provided with flat perforate 7; The shape and the perforate 7 of described plug 5 xsects are consistent, and are provided with boss 8 with illuminating lamp tube 3 contacted ends.
Height according to reactor drum; With what of the amount of the needed illumination of each different growing stage of unit cell algae, make illuminance enough, also can shine the reactor drum various piece; Make it all to receive enough illumination, set up 6～10 illuminating lamp tubes 3 in the above-mentioned lamp bracket (2) from top to bottom.
Situation according to illuminating lamp tube 3 illuminations; The intensity of the illumination that possibly need with unit cell algae in the reactor drum; The spacing of above-mentioned illuminating lamp tube 3 according to the situation of existing illuminating lamp tube 3, and makes and lamp bracket 2 width tablings in 400～600mm scope; In a carrier unit, effectively distribute, the length of illuminating lamp tube 3 is in 600～800mm scope.
For the light that makes 3 of illuminating lamp tubes through the unlatching of constantly combination and different illuminating lamp tubes 3 or close, realization is satisfied the growth of unit cell algae to the illumination of the unit cell algae in the reactor drum, the luminous intensity of illuminating lamp tube 3 is in 200～30000lx scope.
Embodiment
The speed of growth according to cultivation unit cell algae requires different; Can adopt different illumination at the different growing stage of unit cell algae; The growth of unit cell algae successively can be experienced lag phase, exponential phase of growth, allometry decrement phase, stationary phase, five periods of decline phase; Because the unit cell algae of being cultivated promptly gets into gathering system and collects and can not stay for a long time in the bioreactor after reaching bigger density; So generally only need during unit cell algae cultured continuously to experience preceding four periods, also only need regulate to making corresponding illumination these four periods to the illumination of the unit cell algae in the reactor drum.
In order to realize the different demands of intensity of illumination; Can be through the different numbers of illuminating lamp tube in the lamp bracket; With the selection of different capacity illuminating lamp tube, the control through switch in the power control box can realize the unlatching of which fluorescent tube or close, also can select which power fluorescent tube unlatching or close.
Illuminating lamp tube with 8W and 28W is an example, in actual selection, can be chosen at and all carry out illumination by the 8W illuminating lamp tube daytime, can select for use the 28W illuminating lamp tube to carry out illumination at night.
Two of 8W are one group of low light level, and one of 28W is one group of high light, and switch all is set in power control box.
In when inoculation, daytimes 3 high light, evenings 3 high light.Lag phase, daytime 2 high lights, 1 low light level, evening 2 high lights, 1 low light level.Exponential phase of growth, daytimes 3 high light, evenings 3 high light.The allometry decrement phase, daytime 2 high lights, 1 low light level, evenings 2 low light level.Stationary phase, daytimes 2 low light level, evening 1 low light level.When results, need not illumination.
Claims (6)
1. the illumination system of a unit cell algae cultured continuously; The lamp bracket (2) that comprises a square frame shape that is connected with power control box (1); It is characterized in that also comprising the illuminating lamp tube (3) that is horizontally set in lamp bracket (2) frame; Lamp bracket (2) internal layout has and power control box (1) wire connecting; And the two ends of illuminating lamp tube (3) are connected with lamp bracket (2) via the plug (5) of wire terminations, and described illuminating lamp tube (3) is provided with the waterproof crown top of burner (4), the switch (6) that power control box (1) inner control illuminating lamp tube (3) is opened or closed with the junction of plug (5).
2. illumination system as claimed in claim 1 is characterized in that the above-mentioned waterproof crown top of burner (4) is the columniform cap-like structure body that one one end opens wide, and its other end is provided with flat perforate (7); The shape and the perforate (7) of described plug (5) xsect are consistent, and are provided with boss (8) with the contacted end of illuminating lamp tube (3).
3. illumination system as claimed in claim 1 is characterized in that setting up from top to bottom in the above-mentioned lamp bracket (2) 6～10 illuminating lamp tubes (3).
4. like claim 1 or 3 described illumination systems, the spacing that it is characterized in that above-mentioned illuminating lamp tube (3) is in 400～600mm scope, and the length of illuminating lamp tube (3) is in 600～800mm scope.
5. like claim 1 or 3 described illumination systems, the luminous intensity that it is characterized in that above-mentioned illuminating lamp tube (3) is in 200～30000lx scope.
6. the method for utilizing the described illumination system of claim 1 in unit cell algae cultured continuously, the unit cell algae to be carried out illumination; Comprise the hanging-bag type bioreactor is hung on the support; Again the hanging-bag type bioreactor is connected with inflation system with feed liquid adding system, algae liquid gathering system respectively; And unit cell algae algae kind is inoculated in the hanging-bag type bioreactor, it is characterized in that further comprising the steps of:
Described illumination system and the hanging-bag type bioreactor that hangs on the support are arranged side by side; Through the quantity of illuminating lamp tube in the on-off control lamp bracket in the power control box, or the illuminating lamp tube of selection different capacity, the intensity of illumination of different demands when being implemented in unit cell algae inoculation back to results, specific as follows:
When inoculation, the intensity of illumination in daytime, evening is controlled at 2000～30000lx scope; At lag phase, the intensity of illumination in daytime, evening is controlled at 1000～30000lx scope; In exponential phase of growth, the intensity of illumination in daytime, evening is controlled at 2000～30000lx scope; At the allometry decrement phase, the intensity of illumination on daytime is controlled at 1000～20000lx scope, and the intensity of illumination in evening is controlled at 600～10000lx scope; In stationary phase, the luminous intensity on daytime is controlled at 600～10000lx scope, and the luminous intensity at night is controlled at 200～6000lx scope; When results, need not illumination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110406198XA CN102492608B (en) | 2011-12-08 | 2011-12-08 | Illumination system for continuously culturing microalgae and illumination method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110406198XA CN102492608B (en) | 2011-12-08 | 2011-12-08 | Illumination system for continuously culturing microalgae and illumination method thereof |
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| CN102492608A true CN102492608A (en) | 2012-06-13 |
| CN102492608B CN102492608B (en) | 2013-11-20 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071133A (en) * | 2010-12-02 | 2011-05-25 | 中国海洋大学 | Hanging bag-type chlamydomonas culturing bracket |
| CN202040788U (en) * | 2011-04-26 | 2011-11-16 | 成都智拓水族用品有限公司 | Water-proof lamp cap of fluorescent lamp tube for aquarium |
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2011
- 2011-12-08 CN CN201110406198XA patent/CN102492608B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071133A (en) * | 2010-12-02 | 2011-05-25 | 中国海洋大学 | Hanging bag-type chlamydomonas culturing bracket |
| CN202040788U (en) * | 2011-04-26 | 2011-11-16 | 成都智拓水族用品有限公司 | Water-proof lamp cap of fluorescent lamp tube for aquarium |
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| CN102492608B (en) | 2013-11-20 |
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