CN102488082A - Paulownia flower extractive feed additives - Google Patents
Paulownia flower extractive feed additives Download PDFInfo
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- CN102488082A CN102488082A CN2011103917625A CN201110391762A CN102488082A CN 102488082 A CN102488082 A CN 102488082A CN 2011103917625 A CN2011103917625 A CN 2011103917625A CN 201110391762 A CN201110391762 A CN 201110391762A CN 102488082 A CN102488082 A CN 102488082A
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Abstract
The invention aims at providing a method for preparing paulownia flower extractive feed additives, which particularly comprises the steps of preparing extractive extract, spray-drying the extractive extract and adding auxiliary materials to achieve compound to obtain the feed additives, namely smashing paulownia flower into crude powder with 5-20 meshes, adding water 4-6 times as weight as the paulownia flower, soaking for 0.5-1 hour, carrying out the first extraction, extracting for 1-2 hours at the temperature under 100 DEG C, filtering, adding water 4-6 times as weight as the paulownia flower, carrying out the second extraction, extracting for 1-2 hours at the temperature under 100 DEG C, combining liquid medicines, and concentrating the liquid medicines into the extract with relative density of 1.10-1.20; spray-drying the extract with technology conditions of the air inlet temperature of 170-190 DEG C, the air outlet temperature of 80-90 DEG C and the air inlet pressure of 40 Mpa, wherein the moisture content of dry powder of prepared extract is smaller than or equal to 5%; and mixing the extract with at least one of carries, such as silica, pine needle meal and attapulgite powder to obtain the paulownia flower extractive feed additives.
Description
Technical field:
The present invention relates to a kind of fortune paulownia flower extract feed additive, belong to field of feed additive technology.
Background technology:
Fortune paulownia flower is the flower of goatweed paulownia, and bitter is cold in nature, goes into lung channel.Contain ursolic acid (ursolic acid), Matteuccia strthiopteris phenol (matteucinol), cupreol (V), daucosterol compositions such as (VI); Wherein ursolic acid has stronger bacteriostasis external to gram-positive bacteria, gram-negative bacteria and saccharomycete, and is wherein stronger to staphylococcus aureus, hay bacillus, micrococcus scarlatinae and type streptococcus effect.Fortune paulownia flower has wind-dispelling heat-dissipating, clearing heat and detoxicating, the merit of clearing liver and improving vision, and is usually used in the treatment of the infection of the upper respiratory tract, Bronchopneumonia, acute tonsillitis, bacillary dysentery, chordapsus, acute conjunctivitis, mumps, furuncle, sore tinea clinically.Add that fortune paulownia flower source is wider, all there is plantation China East China, Central-South, southwest, and be with low cost; Thereby fortune paulownia flower as feed addictive as far back as just being applied in the livestock-raising many years ago; Have the growth of animals or poultry of raising speed, the prevention intestines problem improves effects such as culture benefit.
The trend that presents intensification, scale along with the development of current aquaculture; Directly with the coarse crushing thing of fortune paulownia flower or fortune paulownia flower make an addition to exist in the feed use amount big, stir inhomogeneous; Increase problems such as human input, this makes fortune paulownia flower be restricted in the application of scale raising.
Summary of the invention:
The objective of the invention is to the defective to above-mentioned existence, propose a kind of preparation method of fortune paulownia flower extract feed additive, this product has the active constituent content height, use amount is little, is easy to advantages such as storage, transportation, has adapted to the needs of large-scale cultivation.
Technical solution of the present invention comprises preparation, spray-drying and and composite three aspects of feed addictive that make of auxiliary material of fortune paulownia flower extract medicinal extract.
Following processing step:
One, the preparation of fortune paulownia flower extract medicinal extract: the raw material fortune paulownia flower is ground into 5～20 purpose meal; The water that adds 6～10 times of fortune paulownia flower weight soaks after 0.5～1 hour earlier and extracts first; Extracting temperature is 100 ℃; Extraction time is 1～2 hour, and filtering liquid medicine obtains first filtrating and first filter residue; The water that adds 4～6 times of fortune paulownia flower weight again mixes with first filter residue and carries out the second time and extract; Extracting temperature is 100 ℃; Extraction time is 1～2 hour, and filtering liquid medicine obtains second filtrating and second filter residue; Merge first filtrating and second filtrating, through the concentration tank that reduces pressure soup being condensed into relative density is 1.10～1.20 medicinal extract.
Two, the spray-drying of fortune paulownia flower extract: fortune paulownia flower extract dry powder, moisture in the dry powder≤5% are processed in the spray-dried tower spraying of fortune paulownia flower extract medicinal extract.Spray-dired process conditions are: 170～190 ℃ of tower EATs, 80～90 ℃ of leaving air temps, air intake pressure are 40Mpa.
Three, the preparation of fortune paulownia flower extract feed additive: with making feed addictive after at least a the mixing in fortune paulownia flower extract dry powder and appropriate carriers such as silica, pine needle meal, the attapulgite.
The present invention has following remarkable efficacy: make an addition to the production performance that can significantly improve animal in the animal daily ration for a long time; Strengthen the animal body immunity function and reach adaptive capacity stressors; And can effectively prevent the generation of animal alimentary canal diseases; Because the product of this invention is that the fortune paulownia flower with integration of drinking and medicinal herbs is a raw material, does not contain any antibiotic and chemical synthetic drug, thus its have green, antibiotic-free residual, do not induce advantages such as body develops immunity to drugs.
The practical implementation method:
Embodiment 1:
Get the 100kg fortune paulownia flower, it is ground into 5～20 purpose meal, added 1000kg water logging bubble again 0.5 hour, heating and extracting, temperature are 100 ℃, and extraction time is 1 hour, filtering liquid medicine, the win filtrating and first filter residue; The back adds 600kg water and mixes with first filter residue and carry out the extraction second time; Temperature is 100 ℃; Extraction time is 1 hour, gets second filtrating and second filter residue, merges first filtrating and second filtrating; Application of reduced pressure concentration tank concentrated medicament obtains relative density and is 1.20 fortune paulownia flower extract feed additive medicinal extract.Again the spray-dried tower of medicinal extract is carried out spray drying treatment, 170 ℃ of tower EATs, 80 ℃ of leaving air temps, air intake pressure is 40Mpa, obtains fortune paulownia flower extract dry powder, moisture is 2.38%.Again with fortune paulownia flower extract dry powder with after silica evenly mixes according to weight ratio at 1: 4, make the fortune paulownia flower extract feed additive.
For investigating the safety indexes of fortune paulownia flower extract feed additive; Company has entrusted Yangzhou University's veterinary college pharmacology and toxicity teaching and research room to carry out acute toxicity test, Salmonella reversion test, micronucleus test, traditional teratogenicity test and the short-term feeding trial research in 30 days of product in July, 2010, and concrete test situation is following:
Draw through the oral acute toxicity testing proof of mouse; Death does not appear in fortune paulownia flower extract feed additive test group mouse, does not also find the abnormal response relevant with administration to show this additive oral administration short term security; There is not obvious acute toxic and side effect, to female, male its mouse oral LD
50All, belong to nontoxic rank greater than 30.0g/kg b.w..
Through ames test (Salmonella reversion test); The change clump count that returns that is tried each dose groups of thing fortune paulownia flower extract feed additive does not have dose-response relationship; Shown that fortune paulownia flower extract feed additive Salmonella reversion test result is negative, salmonella typhimurium has not been had mutagenicity.
Through mouse marrow cell micro nuclear test, there is not dose-response relationship between each test group micronuclear rates.According to micronucleus test criterion, the fortune paulownia flower extract feed additive is negative to the mouse marrow cell micro nuclear test result.
Through the rat traditional teratogenicity test, the young number of each dosetest group nest of fortune paulownia flower extract feed additive, the young average weight of tire, body length, placenta weigh, sex when absorbs the tire rate and the normal control group does not have significant difference (P＞0.05).Shown that the fortune paulownia flower extract feed additive does not have teratogenesis to the Wistar rat.
Through 30 days short-term feeding trials of rat, investigated body weight and the food consumption situation of test rat, blood cell and blood parameters, and weighing the heart, liver, spleen, lung, kidney, ovary uterus, testicular weight, calculated organ coefficient; And main organs such as liver,kidney,spleen, stomach, testis, ovary, duodenum, colon are carried out histopathological examination, through the determination data of above each side, shown that the fortune paulownia flower extract feed additive do not observe tangible illeffects to rat.
Through above 5 tests, the fortune paulownia flower extract feed additive that has shown this invention is a kind of safe feed addictive.
Large-scale egg kind chicken breeding enterprise carries out feeding experiment in the Yicheng, Shanxi with this feed addictive that makes; The test chicken crowd is 345 age in days laying hens; The laying rate of laying hen of this age in days is in the laying rate decline stage behind the egg-laying peak; Control group is identical with fortune paulownia flower extract feed additive group chicken crowd's basal diet, and concrete result of the test is as shown in the table:
Visible by last table; The whole 28 days in-test; The control group laying rate has descended 3.51 percentage points, and fortune paulownia flower extract feed additive group has only descended 1.16 percentage points, and is visible; The fortune paulownia flower extract feed additive can significantly slow down the decrease speed of laying rate behind the laying hen egg-laying peak, i.e. the feed addictive of this invention can effectively improve chicken crowd's egg laying performance.Aspect average egg weight, fortune paulownia flower extract feed additive group has also exceeded 1.92g than control group.Because fortune paulownia flower extract feed additive group chicken crowd's health status is better, stronger to the resistance of alimentary canal common disease, the whole test phase death rate of fortune paulownia flower extract feed additive group is merely 1.21%, and control group is up to 2.05%.The feedstuff-egg ratio data show; Fortune paulownia flower extract feed additive group is merely 2.01; And the feedstuff-egg ratio of control group is 2.12; Be significantly higher than fortune paulownia flower extract feed additive group, shown that the fortune paulownia flower extract feed additive can significantly improve body to the absorbing of nutriment, thereby improved food conversion ratio.
Embodiment 2:
Get the 100kg fortune paulownia flower, it is ground into 5～20 purpose meal, added 600kg water logging bubble again 1 hour, heating and extracting, temperature are 100 ℃, and extraction time is 2 hours, filtering liquid medicine, the win filtrating and first filter residue; The back adds 400kg water and mixes with first filter residue and carry out the extraction second time, and temperature is 100 ℃, and extraction time is 1 hour; Get second filtrating and second filter residue; Merge first filtrating and filtrate with second, application of reduced pressure concentration tank concentrated medicament obtains relative density and is 1.15 fortune paulownia flower extract medicinal extract.Again the spray-dried tower of medicinal extract is carried out spray drying treatment, 180 ℃ of EATs, 85 ℃ of leaving air temps, air intake pressure is 40Mpa, obtains fortune paulownia flower extract dry powder, moisture is 2.52%.Again with fortune paulownia flower extract dry powder with after attapulgite evenly mixes according to weight ratio at 1: 5, make the fortune paulownia flower extract feed additive.
The fortune paulownia flower extract feed additive that this method is made is applied in the production of child care pig; Test and Selection 300 of close 35 age in days weanling pigs of same kind, parity basically identical, body weight; Be divided into 5 groups at random, every group of 6 repetitions, each repeats 10 piglets.The control group piglet basal diet (not containing the fortune paulownia flower extract feed additive) of feeding; Handle I and organize the basal diet+350g/t antibiotic of feeding (being that daily ration per ton adds antibiotic 350g); Handle II and organize the basal diet+300g/t fortune paulownia flower extract feed additive of feeding (being that daily ration per ton adds fortune paulownia flower extract feed additive 300g); Handle III and organize the basal diet+400g/t fortune paulownia flower extract feed additive of feeding (being that daily ration per ton adds fortune paulownia flower extract feed additive 400g), handle IV and organize the basal diet+500g/t fortune paulownia flower extract feed additive of feeding (being that daily ration per ton adds fortune paulownia flower extract feed additive 500g).Test 7 days preliminary trial periods, begin to carry out the formal test phase from piglet 42 ages in days, testing total fate is 49 days.Duration of test has been measured production performance, diarrhoea, morbidity and the death state of piglet, anti-oxidant index of blood and haematogenic immunity index.
The each several part result of the test is following:
(1) improves the production performance aspect: compare with control group; Each fortune paulownia flower extract feed additive group (300g/t group, 400g/t group and 500g/t group) all can improve piglet at daily ingestion amount in earlier stage, and wherein the daily ingestion amount of 500g/t group has improved 10.6% (P＜0.05); 400g/t compares control group with the daily gain of 500g/t group also has raising extremely significantly, has improved 16.7% (P＜0.01) and 17.6% (P＜0.01) respectively, and the 300g/t group also has certain effect for the raising of daily gain.The fortune paulownia flower that each level is added all utmost point has reduced piglet significantly in the test material anharmonic ratio in early stage, and wherein best with the effect of 300g/t, its material anharmonic ratio has reduced by 9.8% (P＜0.01).
(2) diarrhoea situation: result of the test shows, adds the fortune paulownia flower extract feed additive in the feed and can make the grice diarrhoea rate reduce by 28.1～35.9% (P＞0.05), and the diarrhoea index reduces by 9.5～38.1% (P＞0.05), and its effect is better than antibiotic.
(3) to the influence of weanling pig serum anti oxidability: fortune paulownia flower extract feed additive 400g/t and 500g/t interpolation group make that the T-SOD vigor has improved 2.1% and 8.9% respectively in the serum, make the Cu-ZnSOD vigor utmost point significantly improve 41.8% (P＜0.01) and 43.4% (P＜0.01).The serum GSH-Px vigor of each fortune paulownia flower extract feed additive group and antibiotic group is compared control group and is improved, and wherein the raising degree of 300g/t and 400g/t fortune paulownia flower extract feed additive group is maximum.Show that the fortune paulownia flower extract feed additive can improve the level of piglet serum anti oxidation relevant enzyme, have the effect of the oxidation resistance that improves body.
(4) to the weanling pig Immune Effects: compare with control group; Fortune paulownia flower extract feed additive 300g/t group and 500g/t group have significantly improved the content (P＜0.05) of IgG in the piglet serum; The 400g/t group also is improved to some extent, and the IgG content of each interpolation group and antibiotic group difference is remarkable (P＞0.05) not.Antibiotic group and 300g/t fortune paulownia flower extract feed additive group make the IgA content of piglet serum all improve 5.4% (P＞0.05).The piglet serum lysozyme activity of fortune paulownia flower extract feed additive group 300g/t group, 400g/t group and 500g/t group has improved 96.5%, 122.5% and 134.7% than control group respectively, has reached the utmost point level of signifiance (P＜0.01).
Embodiment 3:
Get the 100kg fortune paulownia flower, it is ground into 5～20 purpose meal, added 800kg water logging bubble again 0.5 hour, heating and extracting, temperature are 100 ℃, and extraction time is 2 hours, filtering liquid medicine, the win filtrating and first filter residue; The back adds 500kg water and mixes with first filter residue and carry out the extraction second time; Temperature is 100 ℃; Extraction time is 1 hour, gets second filtrating and second filter residue, merges first filtrating and second filtrating; Application of reduced pressure concentration tank concentrated medicament obtains relative density and is 1.10 fortune paulownia flower extract feed additive medicinal extract.Again the spray-dried tower of medicinal extract is carried out spray drying treatment, 190 ℃ of EATs, 90 ℃ of leaving air temps, air intake pressure is 40Mpa, obtains fortune paulownia flower extract dry powder, moisture is 2.45%.Again with fortune paulownia flower extract dry powder with after pine needle meal evenly mixes according to weight ratio at 2: 3, make the fortune paulownia flower extract feed additive.
The fortune paulownia flower extract feed additive that adopting said method is made adds in the 1 Japanese instar chickling daily ration according to the consumption that feed per ton adds 500g; Test divides control group and fortune paulownia flower extract feed additive group; Test period was 8 weeks, and result of the test is as shown in the table:
The result shows, adds 500mg/kg fortune paulownia flower extract feed additive in the feed, compares with control group; Average daily gain has improved 16.7%, and average daily ingestion amount, material anharmonic ratio have reduced by 9.95%, 22.68% respectively; Survival rate has improved 6.18 percentage points, and the raising rate has reached 6.68%.
Claims (4)
1. the preparation method of a fortune paulownia flower extract feed additive, its characteristic comprise fortune paulownia flower extract medicinal extract preparation, fortune paulownia flower extract spray-drying and with the composite content that makes three aspects of feed addictive of auxiliary material.
2. according to claim 1, the preparation technology of fortune paulownia flower extract medicinal extract is: the raw material fortune paulownia flower is ground into 5～20 purpose meal, and the water logging bubble that adds 6～10 times of fortune paulownia flower weight carries out after 0.5～1 hour extracting the first time; Temperature is 100 ℃, extracts filtering liquid medicine 1～2 hour; The water that adds 4～6 times of fortune paulownia flower weight carries out the second time and extracts, and temperature is 100 ℃, extracts 1～2 hour; Merge soup, it is 1.10～1.20 medicinal extract that decompression is condensed into relative density.
3. according to claim 1, the spray-dired process conditions of fortune paulownia flower extract are: EAT 170-190 ℃, leaving air temp 80-90 ℃, air intake pressure is 40Mpa, fortune paulownia flower extract dry powder moisture≤5% that makes.
4. according to claim 1 and the composite feed addictive that makes of auxiliary material are specially and at least aly in fortune paulownia flower extract dry powder that spray-drying is made and carrier such as silica, pine needle meal, the attapulgite make the fortune paulownia flower extract feed additive after mixing by a certain percentage.
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| CN104839479A (en) * | 2015-04-30 | 2015-08-19 | 中粮饲料有限公司 | Sow daily ration and application thereof |
| CN105687648A (en) * | 2014-11-27 | 2016-06-22 | 北京大北农动物保健科技有限责任公司 | Poultry traditional Chinese medicine composition, feed additive and premix |
| CN108570114A (en) * | 2017-11-29 | 2018-09-25 | 江苏农牧科技职业学院 | A kind of preparation and application of fortune paulownia flower polysaccharide Chinese traditional immunopoteniators |
| CN108634098A (en) * | 2018-04-27 | 2018-10-12 | 郑州工程技术学院 | A kind of preparation method of pure green broad-spectrum antiseptic feed addictive |
| CN111011628A (en) * | 2019-12-19 | 2020-04-17 | 何先清 | Plant extract and preparation method and application thereof |
| CN111228359A (en) * | 2020-01-10 | 2020-06-05 | 江苏农牧科技职业学院 | Paulownia flower extract with antioxidant activity in vivo and in vitro |
| CN115137780A (en) * | 2022-07-26 | 2022-10-04 | 河南中医药大学 | Application of paulownia flower extract in preparation of estrogen medicines |
| CN115886127A (en) * | 2022-10-18 | 2023-04-04 | 无锡正大生物股份有限公司 | Preparation method of paulownia flower extract for feed anti-additive and extractor |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105687648A (en) * | 2014-11-27 | 2016-06-22 | 北京大北农动物保健科技有限责任公司 | Poultry traditional Chinese medicine composition, feed additive and premix |
| CN105687648B (en) * | 2014-11-27 | 2020-10-20 | 北京大北农动物保健科技有限责任公司 | Traditional Chinese medicine composition for poultry, feed additive and premix |
| CN104839479A (en) * | 2015-04-30 | 2015-08-19 | 中粮饲料有限公司 | Sow daily ration and application thereof |
| CN108570114A (en) * | 2017-11-29 | 2018-09-25 | 江苏农牧科技职业学院 | A kind of preparation and application of fortune paulownia flower polysaccharide Chinese traditional immunopoteniators |
| CN108634098A (en) * | 2018-04-27 | 2018-10-12 | 郑州工程技术学院 | A kind of preparation method of pure green broad-spectrum antiseptic feed addictive |
| CN111011628A (en) * | 2019-12-19 | 2020-04-17 | 何先清 | Plant extract and preparation method and application thereof |
| CN111228359A (en) * | 2020-01-10 | 2020-06-05 | 江苏农牧科技职业学院 | Paulownia flower extract with antioxidant activity in vivo and in vitro |
| CN115137780A (en) * | 2022-07-26 | 2022-10-04 | 河南中医药大学 | Application of paulownia flower extract in preparation of estrogen medicines |
| CN115886127A (en) * | 2022-10-18 | 2023-04-04 | 无锡正大生物股份有限公司 | Preparation method of paulownia flower extract for feed anti-additive and extractor |
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Application publication date: 20120613 |