KR20100125738A - Bee feed for having improved antibiotic effect using lactobacillus and a method for preparing thereof - Google Patents

Abstract

PURPOSE: A bee feed with the improved antibiotic effect, and a manufacturing method thereof are provided to use active concentrated water for removing active oxygen inside the body of bees. CONSTITUTION: A sugar liquid as bee feed is formed with 3kg of brown sugar, 1.6~2 liters of water, 0.06~.01kg of lactobacillus, and 0.01~0.03kg of oligosaccharide. The lactobacillus is selected from the group consisting of Lactococcus including Streptococcus faecium, Lactobacillus acidophilus, Lactobacillus including Lactobacillus lactis, and Bacillus including Bacillus subtilis. The oligosaccharide is fructooligosaccharide powder.

Description

Bee feed for having improved antibiotic effect using lactobacillus and preparation method thereof

The present invention relates to a beekeeping feed having an improved antibiotic effect using lactic acid bacteria and a method for producing the same, which is treated with lactic acid bacteria to brown sugar and then decolorized to form a white sugar solution, wherein active concentrated water is used to enhance the activation of lactic acid bacteria. The present invention relates to a beekeeping feed and a method of preparing the same, which adds propolis and minerals thereto, removes free radicals in vivo, balances the nutrition of beekeepers, and provides an antibiotic effect without using a separate artificial antibiotic.

Raw sugar is brown when it is made in aqueous solution, and this causes the coloration of honey (dark brown), so beekeepers do not use it when collecting high-quality acacia honey.

However, feeding edible white sugar can lead to nutritional imbalance due to lack of vitamins, proteins, and minerals, which can lead to weakness of the bee group and make it easy to get sick. There is a problem that the economic burden is great.

In addition, the biggest hurdle for bee parasite damage is mite damage. To combat this, formic acid and oxalic acid are used, but it is basically best to keep bees healthy.

In addition, buzzer disease caused by Penibacillus bacteria, which forms endospore and is infected with spores, particularly easy to infect within 24 hours, after 48 hours, the infectivity is reduced to 10%.

Therefore, as a prior art, Korean Patent Application No. 10-2005-0131845 discloses a method of preparing a bee mite remedy feed, but the technology related to the feed manufacturing method is to provide a technology to which artificial antibiotics are added. Similarly, it is not only possible to grow pathogens resistant to artificial antibiotics in the bee's body, but also because of toxicity problems in natural ecosystems.

Commonly used bee artificial antibiotics include the following types.

-Mites Remedies:

1) Product Name: Apistan stripMavrik, Manpo, Pitu (Main Ingredient: Fluvalinate)

2) Product Name: Flovex (Main ingredient: Bromopropylate)

3) Product Name: Perizin (Main Ingredient: Coomaphos)

4) Product Name: Maicut (Main Ingredient: Amitraz)

5) Product Name: Apitol (Main Ingredient: Simiazol)

6) Product Name: Sindeungjeon (Main ingredient: Tetradipon)

7) Product Name: Bayvarol (Main Ingredient: Flumethrin)

8) Product Name: Nonos (Main Ingredient: Salicylic Acid)

9) Product Name: Binenball (Main ingredient: Timol)

Buzzer Remedies:

1) Product Name: Terramycin, Oxytetracycline (Main ingredient Oxytetracycline HCl)

2) Product Name: oximaycin

-Plaster Bottle Remedy Type: Formalin

Artificial antibiotics such as pesticides, preservatives, etc. have a half-life of about 7 days under 34 ° C, 17 days under 30 ° C, and about 13 weeks under 20 ° C.

Accordingly, Korean Patent Application No. 10-1999-0056929 discloses a technology related to bee mite remedies and containers used by gelling formic acid as a natural antibiotic instead of artificial antibiotics. However, the remedy and the container-related technology attempted to provide a simple and effective technique for exterminating bee mite while using formic acid, which is a natural ingredient, but this also causes a problem that the formic acid gradually evaporates in the beehive, so that its efficacy decreases over time. Done.

In this regard, the present inventors are able to balance the nutrition of beekeepers and provide an antibiotic effect without using a separate artificial / natural antibiotic to overcome the above-mentioned problems, so that the beekeepers can effectively replace other beekeepers and nutritional supplements on the market. While conducting research on beekeeping feed that can increase farmers' economic benefits, beekeepers have improved antimicrobial effects by applying lactic acid bacteria to increase their activation and using active concentrated water to remove bee bioactive oxygen. It was confirmed that the feed can be provided and came to complete the present invention.

That is, an object of the present invention is to provide a beekeeping sugar solution that can activate lactic acid bacteria by using lactic acid bacteria and active concentrated water.

Another object of the present invention is to provide a bee feed that can be removed to the active oxygen in vivo by using the above-mentioned beekeeping sugar solution as an active ingredient.

According to the first aspect of the present invention,

It provides a beekeeping sugar solution that enhances the activation of lactic acid bacteria consisting of 1.60-2.00 liters of water, lactic acid bacteria 0.06-0.10 kg, oligosaccharide 0.01-0.03 kg based on 3 kg of yellow sugar.

According to a second aspect of the present invention,

Propolis and minerals in the beekeeping sugar of the first aspect as an active ingredient, wherein the content of each component is formulated in a bee sugar 98.00 ~ 99.9% by weight, propolis 0.05 ~ 1.00% by weight and minerals 0.05 ~ 1.00% by weight It provides a beekeeping feed that removes free radicals in vivo.

According to the third aspect of the present invention,

The beekeeping sugar and pollen of the first aspect as an active ingredient, wherein the content of each component to remove the active oxygen in vivo, characterized in that it is formulated in a beekeeping sugar solution 90.00 ~ 95.00% by weight, and pollen 5.00 ~ 10.00% by weight. Provide bee feed.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

In the present invention, as a beekeeping sugar solution to enhance the activation of lactic acid bacteria, it is characterized by consisting of 1.60-2.00 liters of water, lactic acid bacteria 0.06-0.10 kg, oligosaccharide 0.01-0.03 kg based on 3 kg of yellow sugar.

At this time, the lactic acid bacteria, such as lactic acid bacteria such as Streptococcus pessium, lactobacillus asidophilus, lactobacillus lactis such as lactobacillus, Bacillus subcillus Bacillus subtilis, it is preferable to use, specifically, the lactic acid bacteria 1g standard Streptococcus pesium 2.2x10 ⁹ , 2x10 ⁹ Lactobacillus asidophilus in 1g, 1x10 ⁸ Lactobacillus lactis in 1g basis, 2x10 ⁸ Bacillus subsilus in 1g mixture, 0.08kg After preparing 3kg of white molasses and 0.02kg of powder fructooligosaccharide, a total of 4kg of beekeeping feed was mixed with 0.2 liters of active concentrated water, which consumes the active oxygen in the intestine and lowers the redox power. It is preferable from the viewpoint of producing vitamins and the like.

In addition, in order to promote the activation of lactic acid bacteria, it is preferable to use active concentrated water that maximizes the content of SiO ₃ through the transfer transfer method of discharging and transferring the natural mineral Si, and miniaturizing the crust with a pressurization and magnetization apparatus. .

In particular, the above-mentioned active concentrated water is more preferably used by mixing a small amount of natural pollen, brewer's yeast, meju flour and the like so as to impart bioactive oxygen removal. At the time of mixing, it is sufficient that the natural flower pot, beer yeast, and meju powder are formulated to less than 20 parts by weight based on 100 parts by weight of active concentrated water.

In addition, the oligosaccharide is not limited thereto, but powdered fructooligosaccharide is preferably used in consideration of ease of use.

The yellow molasses is preferably used as a white molasses through a decolorization treatment to overcome the poor marketability due to coloring such as high-grade acacia honey, more specifically injecting compressed air into the yellow molasses through a compressor and in a filter press The cake can be crushed and then dried and collected by stirrer, belt type continuous washer, and purified as white molasses.

Furthermore, when a bee sugar obtained in this way is blended with propolis and minerals, a bee feed for removing active oxygen in vivo can be prepared. In this case, the compounding amount is sufficient in consideration of the antimicrobial effect of beekeeping and economical 98.00 ~ 99.90% by weight, bee Propolis 0.05 ~ 1.00% by weight and minerals 0.05 ~ 1.00% by weight. Not only this, it is also possible to provide a beekeeping feed for removing active oxygen in vivo as a potted rice cake type by mixing pollen in a beekeeping sugar solution. At this time, it is preferable to use rapeseed pots, sundries pots, green tea pots, and the like, and the blending amount of the compound is sufficient to be added in a bee sac solution of 90.00 to 99.9% by weight, and 5.00 to 10.00% by weight.

The sugar solution and bee feed of the present invention thus obtained are used for the prevention and remedy of bee mite and buzzer, along with the feeding and nutrition of bees, early spring-February, late summer-August-August, It is possible to feed when ticks and buzzers are prevalent, as well as late October-November winter specifications.

Sugar and beekeeping feed according to the present invention, as identified through the following examples, bee mite and buzzer is eliminated without the use of separate artificial antibiotics or natural antibiotics, as well as cost-effectiveness and is preferable in terms of palatability It has no inferiority as a feed, and it has the advantage that even if no artificial or natural antibiotics are used, the antibiotic effect can be obtained by energizing the beacons healthy.

According to the present invention, the treatment of lactic acid bacteria in the yellow molasses at this time to promote the activation of lactic acid bacteria by using the active concentrated water and to add the propolis and minerals to this to remove the active oxygen in vivo to balance the nutrition of beekeeping and separate artificial By providing antibiotics without the use of natural antibiotics, it can effectively replace other bee foods and nutritional supplements on the market and increase the economic benefits of bee farms.

Example 1 Preparation of Beekeeping Sugar Solution

The present embodiment is to provide a production example for producing a beekeeping sugar solution.

First, active concentrated water was prepared by the following method: water as active concentrated water which maximized the content of SiO ₃ through the transfer-transfer method in which the natural mineral Si was displaced and transferred to a pressurized and magnetized device to refine the crust. Obtained.

In addition, the yellow molasses was injected into the compressed air through a compressor, crushed the cake in the filter press, and then dried and collected by a stirrer, a belt-type continuous washing machine in order to refine the white molasses through the white molasses step sequentially.

0.2 liters of the concentrated concentrate thus prepared was mixed with 3 kg of white molasses, lactobacillus, asidophilus, lactobacillus, lactobacillus, Bacillus subtilis, 0.08 kg of fructose oligosaccharide and 0.02 kg of fructooligosaccharide, and treated at 40 ° C. for 48 hours. A bee saccharide solution in which lactobacillus was activated was prepared as an experimental example.

Specifically, the lactic acid bacteria is Streptococcus pesium of 2.2x10 ⁹ on 1g, 2x10 ⁹ Lactobacillus asidophilus in 1g, 1x10 ⁸ Lactobacillus lactis on 1g basis, 2x10 ⁸ Bacillus subsil on 1g basis The russ was mixed to prepare 0.08 kg, and then, 3 kg of the white molasses and 0.02 kg of the powdered fructooligosaccharide were mixed to obtain a total of 4 kg of beekeeping feed mixed with 0.2 liters of the active concentrate.

In order to examine the degree of the activated lactic acid bacteria in the present embodiment, using a general water instead of the active concentrated water was prepared in a beekeeping sugar solution in the same manner as described in Comparative Example 1.

In addition, a beekeeping sugar solution prepared using only active concentrated water without using lactic acid bacteria was used as Comparative Example 2.

Experimental Examples and Comparative Examples 1 and 2 were fed to beehives a total of 6 bees per 1,000 bees per 1,000 bees, and then observed the degree of incidence of bee mites and buzzers, and the average number of bees at the beginning of the experiment. , 1 month after the experiment, and summarized in Table 1 below every six months after the experiment.

Experimental Example-Sugar Comparative Example 1-Sugar Comparative Example 2-Sugar Average number (early experiment) 1,000 1,000 1,000 Average number of months (after 1 month) 1,150 1,100 1,100 Average number (after 6 months) 1,400 1,050 1,300

As shown in Table 1, when the lactic acid bacteria of the experimental example according to the present invention was used with active concentrated water, the degree of activation of the lactic acid bacteria was found in Comparative Example 1 (when using lactic acid bacteria but normal water) and It can be clearly seen that it is much improved than in Comparative Example 2 (when the active concentrated water alone without lactic acid bacteria).

In addition, the onset of bee mite was recognized in both Comparative Examples 1 and 2, and in the case of Comparative Example 1, more than 100 outbreaks were recognized.

Example 2 Preparation of Beekeeping Feed 1

Experimental Example Sugar obtained in Example 1 4kg of 0.04kg of propolis and 0.04kg of minerals were respectively blended to prepare a bee feed for the removal of free radicals in vivo. In addition, for comparison, Comparative Examples 1 and 2 in Example 1, 4 kg of each white sugar solution were blended with 0.04 kg of propolis and 0.04 kg of mineral, respectively, to prepare a bee feed.

Experimental Examples and Comparative Examples 1 and 2 were fed to beehives a total of 6 bees per 1,000 bees per 1,000 bees, and then observed the degree of incidence of bee mites and buzzers, and the average number of bees at the beginning of the experiment. , 1 month after the experiment, and 6 months after the experiment are summarized in Table 2 below.

Experimental Example- Beekeeping Feed 1 Comparative Example 1 Beekeeping Feed 1 Comparative Example 2 Beekeeping Feed 1 Average number (early experiment) 1,000 1,000 1,000 Average number of months (after 1 month) 1,200 1,200 1,200 Average number (after 6 months) 1,500 1,150 1,400

As shown in Table 2, when the lactic acid bacteria of the experimental example according to the present invention was used in combination with active concentrated water, the degree of active oxygen removal in vivo was comparative example 1 (when using lactic acid bacteria but using general water) ) And Comparative Example 2 (when the active concentrate was used alone without lactic acid bacteria) was much improved. In addition, the incidence of bee mites was recognized in Comparative Examples 1 and 2, and in particular, in the case of Comparative Example 1, 300 or more inventions were recognized.

In addition, in order to prepare for the antimicrobial effect against bee mites, 4 kg of beekeeping feed with 1000 ml of Binenball as an artificial antibiotic was fed under the same conditions (control example 1) and 4 kg of beekeeping feed with 1000 ml of formic acid as a natural antibiotic. The antibiotic effect between the case of rapid feeding under the same conditions (Control 2) and the present experimental example was observed and summarized in Table 3 below.

Experimental Example- Beekeeping Feed 1 Control Example 1
When using artificial antibiotics Comparative Example 2-
When using formic acid Average number (early experiment) 1,000 1,000 1,000 Average number of months (after 1 month) 1,200 1,210 1,180 Average number (after 6 months) 1,500 1,490 1,480

As shown in Table 3, when the lactic acid bacteria of the experimental example according to the present invention was used with active concentrated water, the antibiotic effect observed from the number of bees was compared with Control Example 1 (when using Binenball as artificial antibiotic) and Control Example 2 ( When formic acid was used as a natural antibiotic, the antibiotic activity was similar to that of the same).

Example 3: Production of Beekeeping Feed 2

Experimental Example 1 in Example 1 White sugar solution 4kg was mixed with 0.44kg of rapeseed pollen as a pollen to prepare a bee feed for removing active oxygen in vivo. In addition, for the sake of comparison, Comparative Example 1 and 2 in Example 1, each of the white sugar solution was formulated with the same rapeseed pollen as a pollen to prepare a beekeeping feed.

After feeding to 1,000 bees under the same conditions as in Example 2, the results of bee mite and buzzer control were visually observed with the number of bees and summarized as Table 4 below.

Experimental Example- Beekeeping Feed 2 Comparative Example 1 Beekeeping Feed 2 Comparative Example 2 Beekeeping Feed 2 Average number (early experiment) 1,000 1,000 1,000 Average number of months (after 1 month) 1,250 1,250 1,250 Average number (after 6 months) 1,600 1,200 1,430

As shown in Table 4, when the lactic acid bacteria of the experimental example according to the present invention were used with active concentrated water, the degree of removal of active oxygen in vivo observed from the number of bees was comparative example 1 (when using lactic acid bacteria but normal water) It can be clearly seen that compared with Comparative Example 2 (when active concentrated water alone without lactic acid bacteria).

In addition, in order to prepare for an antibiotic effect against buzzer disease, a bee feed prepared by adding 200 mg of teramicin (TSP, Pfizer) as an artificial antibiotic to 30 g sugar solution was fed under the same conditions (control example 1) and formic acid as a natural antibiotic. When the bee feed with 200 mg was fed under the same conditions (control 2) and the antimicrobial effect on the buzzer disease between the experimental examples was observed as the average number of animals and the results are summarized in Table 5 below.

Experimental Example- Beekeeping Feed 2 Control Example 1
When using artificial antibiotics Comparative Example 2-
When using formic acid Average number (early experiment) 1,000 1,000 1,000 Average number of months (after 1 month) 1,250 1,2500 1,180 Average number (after 6 months) 1,560 1,490 1,450

As shown in Table 5, the antibiotic effect observed when the lactic acid bacteria of the experimental example according to the present invention were used together with active concentrated water were compared with Comparative Example 1 (when teramicin was used as artificial antibiotic) and Comparative Example 2 (formic acid as a natural antibiotic). When used, it was confirmed that the antibiotic activity was similar or slightly higher.

Claims (8)

Beekeeping sugar solution for enhancing the activation of lactic acid bacteria, characterized in that consisting of 1.60-2.00 liters of water, lactic acid bacteria 0.06-0.10kg, oligosaccharide 0.01-0.03kg based on 3kg per yellow sugar. The sugar solution for beekeeping according to claim 1, wherein the lactic acid bacteria is selected from lactic acid bacteria such as Streptococcus pessium, lactobacillus such as Lactobacillus asidophilus and Lactobacillus lactis, and Bacillus subcillus Bacillus subtilis. The sugar solution for beekeeping according to claim 2, wherein the oligosaccharide is powder fructooligosaccharide. The method of claim 1, wherein the yellow molasses is injected into the compressed air through a compressor, crushed the cake in the filter press, dried and collected by a stirrer, a belt type continuous washing machine to be used as a white molasses decolorizing treatment Beekeeping sugar. Beekeeping feed for removing the active oxygen in vivo formed by combining propolis and minerals in the beekeeping sugar of claim 4. 6. The beekeeping feed of claim 5, wherein the compounding amount is 98.00 to 99.9% by weight of bee sugar, 0.05 to 1.00% by weight of propolis, and 0.05 to 1.00% by weight of minerals. Beekeeping feed for removing the active oxygen in vivo by mixing the potted plant with the sugar solution for beekeeping of claim 4. [9] The beekeeping feed according to claim 7, wherein the compounding amount is 90.00 to 95.00% by weight of beekeeping sugar, and 5.00 to 10.00% by weight of pollen.