CN102472747B - 单克隆抗体的纯化 - Google Patents

单克隆抗体的纯化 Download PDF

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Publication number
CN102472747B
CN102472747B CN201080030495.2A CN201080030495A CN102472747B CN 102472747 B CN102472747 B CN 102472747B CN 201080030495 A CN201080030495 A CN 201080030495A CN 102472747 B CN102472747 B CN 102472747B
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China
Prior art keywords
antibody
solid support
series
antibodies
beads
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Chinese (zh)
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CN102472747A (zh
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高谦
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201080030495.2A 2009-07-08 2010-07-07 单克隆抗体的纯化 Active CN102472747B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22398409P 2009-07-08 2009-07-08
US61/223,984 2009-07-08
PCT/US2010/041146 WO2011005818A1 (en) 2009-07-08 2010-07-07 Purification of monoclonal antibodies

Publications (2)

Publication Number Publication Date
CN102472747A CN102472747A (zh) 2012-05-23
CN102472747B true CN102472747B (zh) 2015-04-29

Family

ID=43427937

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080030495.2A Active CN102472747B (zh) 2009-07-08 2010-07-07 单克隆抗体的纯化

Country Status (6)

Country Link
US (1) US9395370B2 (enExample)
EP (1) EP2452196B1 (enExample)
JP (1) JP5739419B2 (enExample)
CN (1) CN102472747B (enExample)
CA (1) CA2767167A1 (enExample)
WO (1) WO2011005818A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2542576T (pt) 2010-03-02 2016-07-14 Seattle Genetics Inc Métodos para rastreio de anticorpos
CN104034899B (zh) * 2014-07-01 2015-12-30 上海理工大学 一种食源性致病菌杂交瘤细胞快速筛选抗原宏阵列

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891647A (en) * 1994-04-14 1999-04-06 Pasteur Sanofi Diagnostics Method for the immunoassay of antithrombin III activated by a glycosaminoglycan, corresponding monoclonal antibodies and method for obtaining them
CN1446050A (zh) * 2000-06-20 2003-10-01 分子分级公司 蛋白质表达的图析
US6770445B1 (en) * 1999-02-26 2004-08-03 Pacific Northwest Research Institute Methods and compositions for diagnosing carcinomas
US20070237713A1 (en) * 2006-04-04 2007-10-11 Fan Rong A PCan065 Antibody Compositions and Methods of Use

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1072500A (ja) * 1996-06-18 1998-03-17 Mitsui Petrochem Ind Ltd 分子量約２００００のヒト成長ホルモンに特異的に反応するモノクローナル抗体、該抗体を産生する細胞株及び該抗体を用いる分子量約２００００のヒト成長ホルモンの免疫測定方法
US5892687A (en) 1997-03-12 1999-04-06 Ambit Design Systems, Inc. Don't-- care logic
US7271009B1 (en) 1997-11-18 2007-09-18 Bio-Rad Laboratories, Inc. Multi-analyte diagnostic test for thyroid disorders
IT1308253B1 (it) 1999-03-16 2001-12-10 Molteni L E C Dei Flii Alitti Dosatore di precisione per liquidi.
JP2004537712A (ja) * 2000-10-18 2004-12-16 バーチャル・アレイズ・インコーポレーテッド 多重細胞分析システム
US20030143612A1 (en) * 2001-07-18 2003-07-31 Pointilliste, Inc. Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
US20030044849A1 (en) * 2001-08-22 2003-03-06 Steven Kessler Methods for screening monoclonal antibodies on heterogeneous antigen substrates
WO2005116076A2 (en) 2004-01-26 2005-12-08 Debiovision Inc. Neoplasm-specific polypeptides and their uses
JP4680607B2 (ja) * 2005-01-14 2011-05-11 公立大学法人大阪府立大学 シガトキシン類を認識するモノクローナル抗体、およびそれを用いるシガトキシン類検出キット
PE20130588A1 (es) 2007-02-02 2013-05-21 Amgen Inc Hepcidina, antagonistas de la hepcidina y metodos de uso

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891647A (en) * 1994-04-14 1999-04-06 Pasteur Sanofi Diagnostics Method for the immunoassay of antithrombin III activated by a glycosaminoglycan, corresponding monoclonal antibodies and method for obtaining them
US6770445B1 (en) * 1999-02-26 2004-08-03 Pacific Northwest Research Institute Methods and compositions for diagnosing carcinomas
CN1446050A (zh) * 2000-06-20 2003-10-01 分子分级公司 蛋白质表达的图析
US20070237713A1 (en) * 2006-04-04 2007-10-11 Fan Rong A PCan065 Antibody Compositions and Methods of Use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Production and characterization of monoclonal and polyclonal antibodies to human α2-HS：development of a two-site ELISA test;C.Akhoundi et al;《Journal of Immunological Methods》;19941231;第172卷;191页右栏第6-倒数第8行，192页左栏第8行至193页左栏第2行 *

Also Published As

Publication number Publication date
EP2452196A1 (en) 2012-05-16
EP2452196A4 (en) 2013-03-06
US20110009287A1 (en) 2011-01-13
CA2767167A1 (en) 2011-01-13
JP2012532876A (ja) 2012-12-20
EP2452196B1 (en) 2017-09-06
WO2011005818A1 (en) 2011-01-13
US9395370B2 (en) 2016-07-19
CN102472747A (zh) 2012-05-23
JP5739419B2 (ja) 2015-06-24

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