JP5739419B2 - モノクローナル抗体の精製方法 - Google Patents

モノクローナル抗体の精製方法 Download PDF

Info

Publication number
JP5739419B2
JP5739419B2 JP2012519690A JP2012519690A JP5739419B2 JP 5739419 B2 JP5739419 B2 JP 5739419B2 JP 2012519690 A JP2012519690 A JP 2012519690A JP 2012519690 A JP2012519690 A JP 2012519690A JP 5739419 B2 JP5739419 B2 JP 5739419B2
Authority
JP
Japan
Prior art keywords
antibody
solid support
array
antibodies
beads
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012519690A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012532876A5 (enExample
JP2012532876A (ja
Inventor
チエン ガオ
チエン ガオ
Original Assignee
バイオ−ラッド ラボラトリーズ インコーポレーティッド
バイオ−ラッド ラボラトリーズ インコーポレーティッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by バイオ−ラッド ラボラトリーズ インコーポレーティッド, バイオ−ラッド ラボラトリーズ インコーポレーティッド filed Critical バイオ−ラッド ラボラトリーズ インコーポレーティッド
Publication of JP2012532876A publication Critical patent/JP2012532876A/ja
Publication of JP2012532876A5 publication Critical patent/JP2012532876A5/ja
Application granted granted Critical
Publication of JP5739419B2 publication Critical patent/JP5739419B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065Purification, fragmentation
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/04Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2012519690A 2009-07-08 2010-07-07 モノクローナル抗体の精製方法 Active JP5739419B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22398409P 2009-07-08 2009-07-08
US61/223,984 2009-07-08
PCT/US2010/041146 WO2011005818A1 (en) 2009-07-08 2010-07-07 Purification of monoclonal antibodies

Publications (3)

Publication Number Publication Date
JP2012532876A JP2012532876A (ja) 2012-12-20
JP2012532876A5 JP2012532876A5 (enExample) 2014-12-04
JP5739419B2 true JP5739419B2 (ja) 2015-06-24

Family

ID=43427937

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012519690A Active JP5739419B2 (ja) 2009-07-08 2010-07-07 モノクローナル抗体の精製方法

Country Status (6)

Country Link
US (1) US9395370B2 (enExample)
EP (1) EP2452196B1 (enExample)
JP (1) JP5739419B2 (enExample)
CN (1) CN102472747B (enExample)
CA (1) CA2767167A1 (enExample)
WO (1) WO2011005818A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2542576T (pt) 2010-03-02 2016-07-14 Seattle Genetics Inc Métodos para rastreio de anticorpos
CN104034899B (zh) * 2014-07-01 2015-12-30 上海理工大学 一种食源性致病菌杂交瘤细胞快速筛选抗原宏阵列

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2718849B1 (fr) * 1994-04-14 1996-06-14 Pasteur Sanofi Diagnostics Procédé d'immunodosage de l'antithrombine III activée par un glycosaminoglycane, anticorps monoclonaux correspondants et leur procédé d'obtention.
JPH1072500A (ja) * 1996-06-18 1998-03-17 Mitsui Petrochem Ind Ltd 分子量約20000のヒト成長ホルモンに特異的に反応するモノクローナル抗体、該抗体を産生する細胞株及び該抗体を用いる分子量約20000のヒト成長ホルモンの免疫測定方法
US5892687A (en) 1997-03-12 1999-04-06 Ambit Design Systems, Inc. Don't-- care logic
US7271009B1 (en) 1997-11-18 2007-09-18 Bio-Rad Laboratories, Inc. Multi-analyte diagnostic test for thyroid disorders
US6770445B1 (en) * 1999-02-26 2004-08-03 Pacific Northwest Research Institute Methods and compositions for diagnosing carcinomas
IT1308253B1 (it) 1999-03-16 2001-12-10 Molteni L E C Dei Flii Alitti Dosatore di precisione per liquidi.
US6531283B1 (en) * 2000-06-20 2003-03-11 Molecular Staging, Inc. Protein expression profiling
JP2004537712A (ja) * 2000-10-18 2004-12-16 バーチャル・アレイズ・インコーポレーテッド 多重細胞分析システム
US20030143612A1 (en) * 2001-07-18 2003-07-31 Pointilliste, Inc. Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
US20030044849A1 (en) * 2001-08-22 2003-03-06 Steven Kessler Methods for screening monoclonal antibodies on heterogeneous antigen substrates
WO2005116076A2 (en) 2004-01-26 2005-12-08 Debiovision Inc. Neoplasm-specific polypeptides and their uses
JP4680607B2 (ja) * 2005-01-14 2011-05-11 公立大学法人大阪府立大学 シガトキシン類を認識するモノクローナル抗体、およびそれを用いるシガトキシン類検出キット
US20070237713A1 (en) * 2006-04-04 2007-10-11 Fan Rong A PCan065 Antibody Compositions and Methods of Use
PE20130588A1 (es) 2007-02-02 2013-05-21 Amgen Inc Hepcidina, antagonistas de la hepcidina y metodos de uso

Also Published As

Publication number Publication date
EP2452196A1 (en) 2012-05-16
EP2452196A4 (en) 2013-03-06
US20110009287A1 (en) 2011-01-13
CA2767167A1 (en) 2011-01-13
JP2012532876A (ja) 2012-12-20
EP2452196B1 (en) 2017-09-06
WO2011005818A1 (en) 2011-01-13
US9395370B2 (en) 2016-07-19
CN102472747B (zh) 2015-04-29
CN102472747A (zh) 2012-05-23

Similar Documents

Publication Publication Date Title
US4420558A (en) Bright field light microscopic method of enumerating and characterizing subtypes of white blood cells and their precursors
EP1759208A1 (en) Identification of antibody-producing cells
EP1554576B8 (en) Identification of high affinity molecules by limited dilution screening
JPWO2018203572A1 (ja) 非特異反応抑制剤
JP5739419B2 (ja) モノクローナル抗体の精製方法
CA2939789C (en) Red blood cell detection
CA2526109C (en) Immune complex-specific antibody for reducing the blank value in array test formats when detecting antibodies of a particular immunoglobulin class that have been bound in an antigen-specific manner
CN110887967A (zh) 一种检测抗体结合能力的方法及其应用
EP0494210A1 (en) Agglutination assay
KR102397684B1 (ko) 항체의 항원 결합 부위에 결합하는 펩티드 및 이를 이용한 분석 방법
TW202342979A (zh) 檢測方法及檢測試劑
JP7372196B2 (ja) マイコプラズマ・ニューモニエの免疫測定方法及び免疫測定器具
US20030044849A1 (en) Methods for screening monoclonal antibodies on heterogeneous antigen substrates
JPH08503770A (ja) 抗hpa抗体の検出
JP2019207248A (ja) 被検対象の検出方法並びにそのための免疫測定器具及びモノクローナル抗体
WO2019039557A1 (ja) ジカウイルスを検出する方法及びキット
CN118818040A (zh) 一种筛选杂交瘤细胞株的方法
JP2002306164A (ja) エボラウイルスを認識するモノクローナル抗体
WO2003028625A2 (en) Methods for screening monoclonal antibodies on heterogeneous antigen substrates
JP2025107986A (ja) イムノアッセイにおける干渉を低減するための方法およびキット
JP2009508087A (ja) モノクローナル抗体試薬
AU633633B2 (en) Agglutination assay
CN114487436A (zh) 一种快速检测杂交瘤细胞是否分泌抗体的试剂盒
KR20030094547A (ko) 모노클로날 항체를 생산하는 하이브리도마 세포주의선별을 위한 모노클로날항체 스크리닝 방법 및하이브리도마 선별용 키트
CA2115438A1 (en) Hla typing

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130221

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130221

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140716

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141014

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20141014

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150325

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150423

R150 Certificate of patent or registration of utility model

Ref document number: 5739419

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250