CN102471761A - 获得肌成纤维细胞的方法 - Google Patents

获得肌成纤维细胞的方法 Download PDF

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Publication number
CN102471761A
CN102471761A CN2010800324369A CN201080032436A CN102471761A CN 102471761 A CN102471761 A CN 102471761A CN 2010800324369 A CN2010800324369 A CN 2010800324369A CN 201080032436 A CN201080032436 A CN 201080032436A CN 102471761 A CN102471761 A CN 102471761A
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CN
China
Prior art keywords
cell
serum
myofibroblast
medium
myofibroblasts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2010800324369A
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English (en)
Chinese (zh)
Inventor
尼古拉斯·雅克·鲁耶
罗伯特·巴塞尔
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ONCOBIOTEK
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ONCOBIOTEK
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Publication of CN102471761A publication Critical patent/CN102471761A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2010800324369A 2009-07-24 2010-07-02 获得肌成纤维细胞的方法 Pending CN102471761A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0955216 2009-07-24
FR0955216A FR2948381B1 (fr) 2009-07-24 2009-07-24 Procede d'obtention de myofibroblastes.
PCT/EP2010/059478 WO2011009706A1 (fr) 2009-07-24 2010-07-02 Procede d'obtention de myofibroblastes

Publications (1)

Publication Number Publication Date
CN102471761A true CN102471761A (zh) 2012-05-23

Family

ID=41460947

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2010800324369A Pending CN102471761A (zh) 2009-07-24 2010-07-02 获得肌成纤维细胞的方法

Country Status (12)

Country Link
US (1) US9243228B2 (https=)
EP (1) EP2456854B1 (https=)
JP (1) JP5758891B2 (https=)
CN (1) CN102471761A (https=)
AU (1) AU2010275678B2 (https=)
CA (1) CA2767929A1 (https=)
ES (1) ES2650664T3 (https=)
FR (1) FR2948381B1 (https=)
IL (1) IL217352A (https=)
MX (1) MX2012001004A (https=)
NZ (1) NZ597728A (https=)
WO (1) WO2011009706A1 (https=)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104096265A (zh) * 2014-07-01 2014-10-15 温州医科大学 一种用于构建人工血管模型的三维收缩模型的制备方法
CN104630134A (zh) * 2015-02-15 2015-05-20 叶中华 阴道上皮细胞培养基及阴道上皮细胞培养方法
CN111500527A (zh) * 2020-06-30 2020-08-07 北京昱龙盛世生物科技有限公司 一种表皮干细胞的分离培养方法
CN115197892A (zh) * 2022-06-13 2022-10-18 浙江省人民医院 一种人甲状腺乳头状癌组织中成纤维细胞的分离培养方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4398948A4 (en) * 2021-09-07 2025-07-09 Wisconsin Alumni Res Found CARDIAC FIBROBLAST-DERIVED EXTRACELLULAR MATRIX

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573937A (en) * 1989-12-07 1996-11-12 Snow Brand Milk Products Co., Ltd. Serum free culture medium
CN101120085A (zh) * 2005-02-11 2008-02-06 诺和诺德医疗保健公司 在包含植物蛋白水解产物的无血清细胞培养液中生产多肽

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060114381A (ko) * 2004-02-13 2006-11-06 레프로셀 인코퍼레이티드 배아줄기세포용 피더세포 작성 배지 및 피더세포

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573937A (en) * 1989-12-07 1996-11-12 Snow Brand Milk Products Co., Ltd. Serum free culture medium
CN101120085A (zh) * 2005-02-11 2008-02-06 诺和诺德医疗保健公司 在包含植物蛋白水解产物的无血清细胞培养液中生产多肽

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BERNSTEIN AM ET AL.: "Urokinase receptor cleavage: a crucial step in fibroblast-to-myofibroblast differentiation", 《MOL BIOL CELL.》 *
GRUPP CLEMENS ET AL: "A novel model to study renal myofibroblast formation in vitro", 《KIDNEY INTERNATIONAL》 *
JESTER J V ET AL: "Induction of alpha-smooth muscle actin expression and myofibroblast transformation in cultured corneal keratocytes", 《CORNEA》 *
KHOUW ILSE M S L ET AL: "TGF-beta and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro", 《BIOMATERIALS》 *
MICERA A ET AL: "Nerve Growth Factor (NGF) Activates Conjunctival Myofibroblasts: a Role for NGF in Healing Processes and Tissue Remodeling", 《ARVO ANNUAL MEETING ABSTRACT SEARCH AND PROGRAM PLANNER》 *
RONNOV-JESSEN ET AL.: "Identification, paracrine generation, and possible function of human breast carcinoma myofibroblasts in culture", 《RONNOV-JESSEN ET AL.》 *
WEBBER MUKTA M ET AL: "A human prostatic stromal myofibroblast cell line WPMY-1: A model for stromal-epithelial interactions in prostatic neoplasia", 《CARCINOGENESIS》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104096265A (zh) * 2014-07-01 2014-10-15 温州医科大学 一种用于构建人工血管模型的三维收缩模型的制备方法
CN104096265B (zh) * 2014-07-01 2016-03-23 温州医科大学 一种用于构建人工血管模型的三维收缩模型的制备方法
CN104630134A (zh) * 2015-02-15 2015-05-20 叶中华 阴道上皮细胞培养基及阴道上皮细胞培养方法
CN111500527A (zh) * 2020-06-30 2020-08-07 北京昱龙盛世生物科技有限公司 一种表皮干细胞的分离培养方法
CN115197892A (zh) * 2022-06-13 2022-10-18 浙江省人民医院 一种人甲状腺乳头状癌组织中成纤维细胞的分离培养方法

Also Published As

Publication number Publication date
US20120190109A1 (en) 2012-07-26
FR2948381B1 (fr) 2013-07-12
FR2948381A1 (fr) 2011-01-28
CA2767929A1 (fr) 2011-01-27
JP5758891B2 (ja) 2015-08-05
AU2010275678B2 (en) 2016-06-16
JP2013500003A (ja) 2013-01-07
EP2456854A1 (fr) 2012-05-30
NZ597728A (en) 2013-11-29
MX2012001004A (es) 2012-05-29
ES2650664T3 (es) 2018-01-19
WO2011009706A1 (fr) 2011-01-27
US9243228B2 (en) 2016-01-26
EP2456854B1 (fr) 2017-08-30
IL217352A0 (en) 2012-02-29
IL217352A (en) 2016-03-31
AU2010275678A1 (en) 2012-02-09

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Application publication date: 20120523