CN102439460B - 多方向微流体装置和方法 - Google Patents

多方向微流体装置和方法 Download PDF

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Publication number
CN102439460B
CN102439460B CN201080021792.0A CN201080021792A CN102439460B CN 102439460 B CN102439460 B CN 102439460B CN 201080021792 A CN201080021792 A CN 201080021792A CN 102439460 B CN102439460 B CN 102439460B
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CN
China
Prior art keywords
medium
binding
sample
microfluidic device
separation
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Expired - Fee Related
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CN201080021792.0A
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English (en)
Chinese (zh)
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CN102439460A (zh
Inventor
艾米·E·赫尔
何梅
侯晨露
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University of California San Diego UCSD
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University of California San Diego UCSD
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
CN201080021792.0A 2009-05-19 2010-05-18 多方向微流体装置和方法 Expired - Fee Related CN102439460B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US17964909P 2009-05-19 2009-05-19
US61/179,649 2009-05-19
US25736109P 2009-11-02 2009-11-02
US61/257,361 2009-11-02
PCT/US2010/035314 WO2010135364A2 (en) 2009-05-19 2010-05-18 Multi-directional microfluidic devices and methods

Publications (2)

Publication Number Publication Date
CN102439460A CN102439460A (zh) 2012-05-02
CN102439460B true CN102439460B (zh) 2015-03-25

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Family Applications (1)

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CN201080021792.0A Expired - Fee Related CN102439460B (zh) 2009-05-19 2010-05-18 多方向微流体装置和方法

Country Status (8)

Country Link
US (1) US9110057B2 (enExample)
EP (1) EP2433143B1 (enExample)
JP (1) JP5747024B2 (enExample)
CN (1) CN102439460B (enExample)
AU (1) AU2010249678B2 (enExample)
CA (1) CA2761698A1 (enExample)
SG (1) SG175739A1 (enExample)
WO (1) WO2010135364A2 (enExample)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9110057B2 (en) 2009-05-19 2015-08-18 The Regents Of The University Of California Multi-directional microfluidic devices and methods
DK2504105T3 (da) 2009-11-24 2021-03-15 Opko Diagnostics Llc Fluidblanding og -afgivelse i mikrofluidsystemer
JP6133780B2 (ja) 2010-11-23 2017-05-24 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 汎捕捉性の結合領域を備えている多方向マイクロ流体デバイス、及びその使用方法
US9029169B2 (en) 2010-12-03 2015-05-12 The Regents Of The University Of California Protein renaturation microfluidic devices and methods of making and using the same
WO2012177940A2 (en) 2011-06-24 2012-12-27 The Regents Of The University Of California Microfluidic devices and methods for separating and detecting constituents in a fluid sample
WO2013089887A2 (en) * 2011-09-30 2013-06-20 The Regents Of The University Of California Microfluidic devices and methods for assaying a fluid sample using the same
CN102886280B (zh) * 2012-08-28 2014-06-11 博奥生物有限公司 一种微流控芯片及其应用
EP3257952B1 (en) 2012-09-11 2020-02-12 Life Technologies Corporation Nucleic acid amplification
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
US10393701B2 (en) 2012-10-08 2019-08-27 The Regents Of The University Of California Microfluidic methods of assaying molecule switching and devices for practicing the same
EP3193169B1 (en) * 2012-10-22 2018-12-12 M-Flow Technologies Limited Fluid sensor comprising a resonant cavity made of an electrically conductive composite material
EP3460486A1 (en) * 2012-11-12 2019-03-27 The Regents of The University of California Electrophoretic bar code assay devices and methods for making and using the same
CA2903382A1 (en) * 2013-03-01 2014-09-12 Wave 80 Biosciences, Inc. Methods and systems for enhanced microfluidic processing
US9995412B2 (en) 2013-03-01 2018-06-12 Wave 80 Biosciences, Inc. Long-throw microfluidic actuator
GB2517864B (en) 2013-03-07 2015-12-09 Univ California Electrophoretic separation devices and methods for using the same
EP2972364B1 (en) * 2013-03-12 2018-05-09 The Regents Of The University Of Michigan Microfluidic device for immunoblotting
US9671368B2 (en) 2013-05-10 2017-06-06 The Regents Of The University Of California Two-dimensional microfluidic devices and methods of using the same
WO2015048168A1 (en) 2013-09-24 2015-04-02 The Regents Of The University Of California Producing a separation medium using grayscale mask
EP3060916B1 (en) * 2013-10-22 2019-07-10 The Regents of The University of California Microfluidic assay devices and methods for making and using the same
EP3149476B1 (en) 2014-05-30 2022-02-16 The Regents of The University of California Subcellular western blotting of single cells
US10852272B2 (en) 2014-06-05 2020-12-01 The Regents Of The University Of California Devices and methods using pore size modulation for detecting analytes in a fluid sample
WO2017044614A1 (en) 2015-09-11 2017-03-16 The Regents Of The University Of California Isoelectric focusing arrays and methods of use thereof
TWI570243B (zh) * 2015-11-03 2017-02-11 國立清華大學 噬菌體之篩選裝置及方法
KR20180081538A (ko) 2015-11-10 2018-07-16 우드햄 바이오테크놀로지 홀딩스, 엘엘씨 전도성 중합체들을 이용한 겔 전기영동 및 전달 조합 및 사용 방법
US9702851B1 (en) 2016-06-17 2017-07-11 Woodham Biotechnology Holdings, LLC Gel electrophoresis and transfer combination using conductive polymers and method of use
KR102088277B1 (ko) * 2018-04-27 2020-03-13 주식회사 유니언스진 면역 화학 진단법을 이용한 표적 항원 검출용 종이기반 3차원 구조의 미세칩과 이를 이용한 표적항원 검출 방법
CN111686826B (zh) * 2019-03-15 2023-05-23 国家纳米科学中心 分层结构的微流控芯片及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000073799A1 (en) * 1999-06-01 2000-12-07 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
WO2002086332A1 (en) * 2001-04-20 2002-10-31 Nanostream, Inc. Porous microfluidic valves
US20030127331A1 (en) * 2002-01-10 2003-07-10 Leka George T. Two-dimensional electrophoresis method and cassette
US20050106740A1 (en) * 2003-11-13 2005-05-19 Boyes Barry E. Methods, systems and devices for performing analytical protocols

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5314804A (en) * 1992-03-24 1994-05-24 Serim Research Corporation Test for Helicobacter pylori
US5637469A (en) * 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
DE4244082C2 (de) * 1992-12-24 1994-11-03 Etc Elektrophorese Technik Wes Verfahren zur hochauflösenden Zweidimensional-Elektrophorese und Vorrichtung zur Durchführung des Verfahrens
US6001229A (en) * 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
DE19856064C2 (de) * 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
US6818112B2 (en) * 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6613581B1 (en) * 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
US7390675B2 (en) * 1999-10-15 2008-06-24 Christopher Feistel Multi-functional and configurable assay
US6784982B1 (en) * 1999-11-04 2004-08-31 Regents Of The University Of Minnesota Direct mapping of DNA chips to detector arrays
US7329388B2 (en) * 1999-11-08 2008-02-12 Princeton Biochemicals, Inc. Electrophoresis apparatus having staggered passage configuration
US6974527B2 (en) * 2000-06-06 2005-12-13 Spectrumedix Llc Multidimensional separations employing an array of electrophoresis channels
US6737260B1 (en) * 2000-09-29 2004-05-18 The United States Of America, As Represented By The Secretary Of Agriculture Sequences encoding PhzO and methods
US7235389B2 (en) * 2001-04-23 2007-06-26 Samsung Electronics Co., Ltd. Molecular detection device and chip including MOSFET
US6974526B2 (en) * 2001-05-01 2005-12-13 Calibrant Biosystems, Inc. Plastic microfluidics enabling two-dimensional protein separations in proteome analysis
US7641780B2 (en) * 2001-05-01 2010-01-05 Calibrant Biosystems, Inc. Two-dimensional microfluidics for protein separations and gene analysis
EP1442131A4 (en) * 2001-10-19 2006-06-07 Univ West Virginia MICROFLUIDIC SYSTEM FOR PROTEOMIC ANALYSIS
DE10161577B4 (de) * 2001-12-14 2008-01-10 Scil Proteins Gmbh Verfahren zur Renaturierung von Proteinen
US7112444B2 (en) * 2002-04-24 2006-09-26 Wisconsin Alumni Research Foundation Method of performing gradient-based assays in a microfluidic device
JP2004061319A (ja) 2002-07-29 2004-02-26 Kawamura Inst Of Chem Res マイクロ流体デバイス及びその使用方法
US20040112751A1 (en) 2002-08-26 2004-06-17 Jongyoon Han Multidimensional electrophoresis and methods of making and using thereof
TW590982B (en) * 2002-09-27 2004-06-11 Agnitio Science & Technology I Micro-fluid driving device
US20060211055A1 (en) 2002-11-12 2006-09-21 Caliper Life Sciences, Inc. Capture and release assay system and method
WO2004061085A2 (en) * 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
US6969452B2 (en) * 2003-02-28 2005-11-29 Combisep, Inc. Two-dimensional protein separations using chromatofocusing and multiplexed capillary gel electrophoresis
US20060191792A1 (en) * 2003-08-25 2006-08-31 Herr Amy E Method and apparatus for gel electrophoretic immunoassay
KR100852348B1 (ko) * 2003-12-23 2008-08-14 와코 쥰야꾸 고교 가부시키가이샤 분석물 주입 시스템
EP1740284A4 (en) * 2004-03-19 2011-04-06 Perkinelmer Las Inc SEPARATION PLATFORM BASED ON ELECTRO-OSMOSIS SURFACE CHROMATOGRAPHY
JP2006010529A (ja) * 2004-06-25 2006-01-12 Canon Inc 磁性粒子分離装置および分離方法
WO2006102516A2 (en) * 2005-03-23 2006-09-28 California Institute Of Technology Devices exhibiting differential resistance to flow and methods of their use
US7828949B2 (en) 2005-12-08 2010-11-09 Electronics And Telecommunications Research Institute Biomolecule detection device, mobile phone for biomolecule detection, and biomolecule detection method
US9718676B2 (en) * 2006-09-14 2017-08-01 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Polymeric nanopillars and nanotubes, their manufacture and uses
US8329016B1 (en) * 2007-07-30 2012-12-11 Sandia Corporation Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis
US20090194483A1 (en) * 2008-01-31 2009-08-06 Robotti Karla M Microfluidic device having monolithic separation medium and method of use
US9110057B2 (en) 2009-05-19 2015-08-18 The Regents Of The University Of California Multi-directional microfluidic devices and methods
US20130078663A1 (en) 2009-12-02 2013-03-28 Amy E. Herr Pore-Limit Electrophoresis (PLE) Microchannel Assays
WO2011106693A2 (en) 2010-02-26 2011-09-01 The Regents Of The University Of Michigan Microscale western blot
JP6133780B2 (ja) 2010-11-23 2017-05-24 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 汎捕捉性の結合領域を備えている多方向マイクロ流体デバイス、及びその使用方法
US9029169B2 (en) 2010-12-03 2015-05-12 The Regents Of The University Of California Protein renaturation microfluidic devices and methods of making and using the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000073799A1 (en) * 1999-06-01 2000-12-07 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
WO2002086332A1 (en) * 2001-04-20 2002-10-31 Nanostream, Inc. Porous microfluidic valves
US20030127331A1 (en) * 2002-01-10 2003-07-10 Leka George T. Two-dimensional electrophoresis method and cassette
US20050106740A1 (en) * 2003-11-13 2005-05-19 Boyes Barry E. Methods, systems and devices for performing analytical protocols

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Electrokinetic Fluid Control in Two-Dimensional Planar Microfluidic Devices;Margaret A. Lerch et.al;《Anal.Chem》;20071231;第79卷;7485-7491 *
Free-Flow Electrophoresis on an Anodic Bonded Glass Microchip;Bryan R.Fonslow et.al;《Anal. Chem.》;20051231;第77卷;5706-5710 *
High-Speed Free-Flow Electrophoresis on Chip;Chao-Xuan Zhang et.al;《Anal. Chem.》;20031231;第75卷;5759-5766 *
Mircrofluidic self-Patterning of Large-Scale Crystalline...;Yong Zeng et.al;《Angew. Chem.》;20081231;第120卷;6488-6491 *

Also Published As

Publication number Publication date
CN102439460A (zh) 2012-05-02
EP2433143A2 (en) 2012-03-28
AU2010249678B2 (en) 2014-08-28
WO2010135364A3 (en) 2011-02-24
US9110057B2 (en) 2015-08-18
JP2012527622A (ja) 2012-11-08
EP2433143A4 (en) 2012-11-28
JP5747024B2 (ja) 2015-07-08
AU2010249678A1 (en) 2011-11-10
CA2761698A1 (en) 2010-11-25
US20110177618A1 (en) 2011-07-21
SG175739A1 (en) 2011-12-29
WO2010135364A2 (en) 2010-11-25
EP2433143B1 (en) 2016-03-02

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