CN102439460B - 多方向微流体装置和方法 - Google Patents

多方向微流体装置和方法 Download PDF

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Publication number
CN102439460B
CN102439460B CN201080021792.0A CN201080021792A CN102439460B CN 102439460 B CN102439460 B CN 102439460B CN 201080021792 A CN201080021792 A CN 201080021792A CN 102439460 B CN102439460 B CN 102439460B
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CN
China
Prior art keywords
medium
binding
sample
microfluidic device
separation
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Expired - Fee Related
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CN201080021792.0A
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English (en)
Chinese (zh)
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CN102439460A (zh
Inventor
艾米·E·赫尔
何梅
侯晨露
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University of California, San Diego
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University of California, San Diego
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
CN201080021792.0A 2009-05-19 2010-05-18 多方向微流体装置和方法 Expired - Fee Related CN102439460B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US17964909P 2009-05-19 2009-05-19
US61/179,649 2009-05-19
US25736109P 2009-11-02 2009-11-02
US61/257,361 2009-11-02
PCT/US2010/035314 WO2010135364A2 (en) 2009-05-19 2010-05-18 Multi-directional microfluidic devices and methods

Publications (2)

Publication Number Publication Date
CN102439460A CN102439460A (zh) 2012-05-02
CN102439460B true CN102439460B (zh) 2015-03-25

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CN201080021792.0A Expired - Fee Related CN102439460B (zh) 2009-05-19 2010-05-18 多方向微流体装置和方法

Country Status (8)

Country Link
US (1) US9110057B2 (enExample)
EP (1) EP2433143B1 (enExample)
JP (1) JP5747024B2 (enExample)
CN (1) CN102439460B (enExample)
AU (1) AU2010249678B2 (enExample)
CA (1) CA2761698A1 (enExample)
SG (1) SG175739A1 (enExample)
WO (1) WO2010135364A2 (enExample)

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US10393701B2 (en) 2012-10-08 2019-08-27 The Regents Of The University Of California Microfluidic methods of assaying molecule switching and devices for practicing the same
EP3193168B1 (en) 2012-10-22 2018-09-26 M-Flow Technologies Limited Fluid sensor comprising a resonant cavity made of an electrically conductive composite material
EP3460486A1 (en) * 2012-11-12 2019-03-27 The Regents of The University of California Electrophoretic bar code assay devices and methods for making and using the same
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WO2014165185A1 (en) 2013-03-12 2014-10-09 The Regents Of The University Of Michigan Microfluidic device for immunoblotting
US9671368B2 (en) 2013-05-10 2017-06-06 The Regents Of The University Of California Two-dimensional microfluidic devices and methods of using the same
JP6605454B2 (ja) 2013-09-24 2019-11-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア グレースケールマスクを用いた分離媒体の生成
US10088475B2 (en) * 2013-10-22 2018-10-02 The Regents Of The University Of California Microfluidic assay devices and methods for making and using the same
EP3149476B1 (en) 2014-05-30 2022-02-16 The Regents of The University of California Subcellular western blotting of single cells
EP3151939B1 (en) 2014-06-05 2023-08-09 The Regents of The University of California Devices and methods using pore size modulation for detecting analytes in a fluid sample
WO2017044614A1 (en) 2015-09-11 2017-03-16 The Regents Of The University Of California Isoelectric focusing arrays and methods of use thereof
TWI570243B (zh) * 2015-11-03 2017-02-11 國立清華大學 噬菌體之篩選裝置及方法
RU2723936C2 (ru) 2015-11-10 2020-06-18 ВУДХЭМ БАЙОТЕКНОЛОДЖИ ХОДИНГЗ, ЭлЭлСи Комбинация электрофореза в геле и переноса с использованием проводящих полимеров и способ использования
US9702851B1 (en) 2016-06-17 2017-07-11 Woodham Biotechnology Holdings, LLC Gel electrophoresis and transfer combination using conductive polymers and method of use
KR102088277B1 (ko) * 2018-04-27 2020-03-13 주식회사 유니언스진 면역 화학 진단법을 이용한 표적 항원 검출용 종이기반 3차원 구조의 미세칩과 이를 이용한 표적항원 검출 방법
CN111686826B (zh) * 2019-03-15 2023-05-23 国家纳米科学中心 分层结构的微流控芯片及其应用

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Also Published As

Publication number Publication date
JP2012527622A (ja) 2012-11-08
US20110177618A1 (en) 2011-07-21
AU2010249678A1 (en) 2011-11-10
EP2433143A2 (en) 2012-03-28
AU2010249678B2 (en) 2014-08-28
EP2433143A4 (en) 2012-11-28
EP2433143B1 (en) 2016-03-02
WO2010135364A3 (en) 2011-02-24
SG175739A1 (en) 2011-12-29
WO2010135364A2 (en) 2010-11-25
CN102439460A (zh) 2012-05-02
US9110057B2 (en) 2015-08-18
CA2761698A1 (en) 2010-11-25
JP5747024B2 (ja) 2015-07-08

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