CN102405793B - Movable multi-purpose root chamber - Google Patents
Movable multi-purpose root chamber Download PDFInfo
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- CN102405793B CN102405793B CN 201110351901 CN201110351901A CN102405793B CN 102405793 B CN102405793 B CN 102405793B CN 201110351901 CN201110351901 CN 201110351901 CN 201110351901 A CN201110351901 A CN 201110351901A CN 102405793 B CN102405793 B CN 102405793B
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Abstract
The invention discloses a movable multi-purpose root chamber, wherein the upper part and the lower part of an outer wall are respectively provided with a top cover and a bottom support. A central fixing shaft and at least three space division hinges are arranged in the outer wall. The space division hinges are vertically arranged on the bottom support, the outer lateral is connected with the outer wall, the inner side is connected with the central fixing shaft through a sleeve, the top part is provided with a bayonet, the bayonet is provided with a planting cup of which the bottom part is a net-shaped structure, the centre of the bottom part of the cup is connected with the central fixing shaft, and a rubber sealing ring is arranged between the outer wall of the planting cup and the bayonet. In the invention, roots can freely extend, which is good for the uniform distribution of the root system in the root chamber; the specific growth space can be flexibly adjusted according to the experiment purpose; because the movable multi-purpose root chamber comprises multiple room chambers, the different partial root systems of a same plant can be sampled randomly to maximally overcome the influences of accidental factors; and the structure of the root chamber is simple, the arrangement is flexible, the root chamber can be reused, the price is low, and the effect is good, so the growth of the root system can be maximally simulated.
Description
Technical field
The present invention relates to the root system of plant research aspect of field of biology, a kind of specifically movable multipurpose root chamber.
Background technology
It is the important channel and the part of terrestrial ecosystem carbon cycle that root system of plant is breathed, and the data of root system respiration are estimation carbonaceous subterranean storehouses and judge it and the key of atmospheric carbon dioxide source/remittance relation.Because the particularity of root growth environment,, especially estimating aspect the root system respiration root system research short of reliable technological means always.
At present; The device of research root system respiration; Main branch original position and two types of simulations: the original position research of root breath mainly is provided with atmospheric sampling device or carbon isotope spike through the field; Acquisition comprises the soil respiration or the carbon signal data of root system, does not have the breathing of plant root growth soil and the carbon signal data before and after the root growth, indirect Estimation root system of plant breath data through contrast; Comparatively speaking, the simulation approach mainly is indoor, utilizes various nutrient solutions, root chamber plant culturing, behind the acquisition root system, measures the breath data of exsomatize root segment or complete stool root system, directly obtains the breath data of root system.Obviously, in simulation process, the collecting method of root system and growing environment are very big to the result of study influence.Wherein, how to estimate more accurately that root system activity intensity, root system and rhizospheric microorganism interaction under the plant strain growth state are difficult points of these researchs to the data aspect the influence of carbonic acid gas breathing.
2007; Kou Taiji etc. once reported a kind of " root division gas collection growth "; They are divided into two parts symmetrical to traditional root case; And the middle upper portion in two Room adds a little chamber of planting seedlings, and plant is planted in the chamber of planting seedlings, and lets two apertures of root system through the chamber sidewall that plants seedlings be stretched over respectively in the root case of two symmetries to go.When sampling, the earth of a root chamber of flush away as collection chamber, is used to measure the breathing of root system; The earth of another root chamber keeps, and is required as keeping the plant normal physiological on the one hand, on the other hand as contrast, in conjunction with no plant contrast, calculates the contribution that root system and rhizospheric microorganism are opposed mutually and breathe.This device has solved the respiration monitoring difficult problem of root system, root system microorganism, soil body three parts preferably.But this device still has some problems to overcome in design:
1, this growth structure is fixed, and is not easy to operation, and the later stage need rinse out the earth in the collection chamber in measuring the root system respiration process; And the moisture film that blots on the root system to be adhered to, and do not damage root, simultaneously since the control experimental error need; Whole process comprises digs out growth from the field, to washing root, to reinstalling to the field; And accomplish and measure, need in 10 min, accomplish, the design that was provided in 2007 cannot say for sure to demonstrate,prove the high-quality on time completion of this process;
2, growth is a square structure, and root system is done radial distribution on the spot putting around plant, and growth can not well adapted root growth habit, and the growth space is underutilized;
3, the root growth chamber only relies on an aperture to be connected with planting seedlings between the chamber, influences the growth of root inevitably, and root is big in the indoor distribution contingency of two roots simultaneously, might be difficult to obtain abundant live body root and supply to measure and use.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of movable multipurpose root chamber.
The present invention solves the problems of the technologies described above the technical scheme of employing to be: a kind of movable multipurpose root chamber; Comprise outer wall, planting cup, top cover, collet, central fixed shaft and rubber seal; The top of outer wall cylindraceous is provided with top cover, and the bottom is provided with collet, and the center of outer pars intramuralis is provided with the lower end and is fixed on the central fixed shaft on the collet; Central fixed shaft is provided with screw thread, and is furnished with nut; Also be provided with at least three space segmentation loose-leaves in the outer wall, the height of space segmentation loose-leaf equates that with outer wall the space segmentation loose-leaf is vertically set on the collet; The outside and the outer wall of space segmentation loose-leaf join, and the inboard sleeve pipe that passes through is connected with central fixed shaft, the bayonet socket that the inboard top of space segmentation loose-leaf is provided with; Planting cup is arranged on the bayonet socket; The cup end of planting cup is a network structure, and the Center-to-Center fixed axis at the cup end connects, and is provided with rubber seal between the outer wall of planting cup and the bayonet socket; Be equipped with sealing rubber pad between space segmentation loose-leaf and outer wall, top cover and the collet;
Top cover and collet are disc structure, and its surface is provided with through hole, and the center of top cover also is provided with the hole of placing planting cup; Collet is provided with back-up ring, and the outer rim of back-up ring overlaps with the outer rim of collet, and the bottom in the collet outside also is provided with base;
Among the present invention, the quantity of space segmentation loose-leaf is 4 ~ 6, and the sleeve position on all space segmentation loose-leaves does not all overlap; Upper and lower at the space segmentation loose-leaf is respectively equipped with circular hole, is provided with the location sieve of cutting apart loose-leaf in order to fixed space in the circular hole, and the outer surface of location sieve is provided with screw thread, and is furnished with nut.
In the present invention, collet has base, makes collet and horizontal plane keep certain space, satisfies the needs of experimental implementation.
Beneficial effect: among the present invention, root can freely stretch, and is beneficial to root system in the indoor even distribution of root; Can be according to concrete growing space, experiment purpose flexible root chamber; Owing to form by a plurality of chambers, can realize different piece root system stochastical sampling to same strain plant, farthest overcome the accidentalia influence; The root cell structure is simple, is provided with flexibly, can repeat to be beneficial to, and is cheap, respond well, can simulate root growth to greatest extent.
Description of drawings
Fig. 1 is a longitudinal sectional drawing of the present invention;
Fig. 2 is the structure chart of space segmentation loose-leaf;
Fig. 3 is the structure chart of top cover;
Fig. 4 is the structure chart of collet;
Fig. 5 is the planting cup structure chart;
Reference numeral: 1, outer wall, 2, planting cup, 3, the space segmentation loose-leaf, 4, the location sieve, 5, top cover, 6, collet, 7, central fixed shaft, 8, rubber seal, 9, through hole, 10, back-up ring, 11, base, 12, sleeve pipe.
Embodiment
Below in conjunction with accompanying drawing and embodiment the present invention is further described, but protection scope of the present invention is not limited in this, can changes size of the present invention according to actual needs.
Embodiment 1
A kind of movable multipurpose root chamber; Comprise outer wall 1, planting cup 2, top cover 5, collet 6, central fixed shaft 7 and rubber seal 8; The top of outer wall 1 is provided with top cover 5, and the bottom is provided with collet 6, and inner center is provided with the lower end and is fixed on the central fixed shaft 7 on the collet 6;
Wherein: outer wall 1 is cylindric cylinder, and internal diameter 20cm, high 25cm, wall thickness 0.5cm are for the PVC material is processed;
Planting cup 2 internal diameter 5cm, dark 6cm in the cup, wall thickness 0.3cm also processes with the PVC material, and the bottom is the network structure of aperture 0.2cm, and the center is provided with the fixing hole that diameter is 0.5cm; The sealing rubber ring 8 of the high 6cm of planting cup 2 outer ring sets, thick 0.1cm;
Also be provided with 4 space segmentation loose-leaves 3 in the outer wall 1; Space segmentation loose-leaf 3 is the rectangular slab of high 25cm, wide 9.9cm, thick 0.3cm; Space segmentation loose-leaf 3 is vertically set on the collet 6, and a side is connected with outer wall 1, and the opposite side top cuts the bayonet socket of high 5.7cm, wide 2.5cm; The below of bayonet socket is provided with the sleeve pipe 12 that internal diameter is 0.5cm, thick 0.1cm, long 3cm, and sleeve pipe 12 positions on each space segmentation loose-leaf 3 all do not overlap; Be provided with central fixed shaft 7 in the sleeve pipe 12, central fixed shaft 7 is processed for the stainless steel rebar of external diameter 0.5cm, long 20.5cm, and the outside is provided with screw thread, and through nut space segmentation loose-leaf 3 and planting cup 2 is fixed on the collet 6;
Be provided with the circular hole of aperture 0.5cm in the upper and lower of space segmentation loose-leaf 3 respectively apart from limit portion 2cm place, be provided with the location sieve 4 of cutting apart loose-leaf 3 in order to fixed space in the circular hole, the external diameter of location sieve 4 is 0.2cm, and outer surface is provided with screw thread, and is furnished with nut; The wide 0.35cm of the equal ring set of side outside space segmentation loose-leaf 3 decannulations, the sealing rubber pad of thick 0.1cm.
In use, at first be installed to 4 space segmentation loose-leaves 3 on the central fixed shaft 7; On bayonet socket, place planting cup 2; Be through location sieve 4 on the space segmentation loose-leaf 3 with nut, adjust angle after, the nut on the stationary positioned sieve 4; Be installed in the bottom of centralized positioning axle 7 to collet 6, and be connected to the water pipe of external adjustment soil moisture in the through hole 9 on the collet 6; Seal seam everywhere again with commercially available Seal Oil; It is indoor to insert each minute of root chamber to ready planting soil according to requirement of experiment successively, and reaches 20 cm and be standard deeply; Sow seed or seedling in plantation cup 2 bottoms, then insert the thick soil of 1 cm again; After accomplishing above step, set up top cover 5 at last;
After accomplishing above-mentioned processing, can carry out the research of simulated determination and the soil nutrient heterogeneity and the root distribution relation of root breath mensuration, hygrophyte root breath.
One, root breath is measured:
After assembling as requested and adjusting each locellus, plant in the plantation carries out preliminary treatment; When measuring, at first take off top cover 5, take off root chamber outer wall 1 again; Rinse out the earth of the root chamber of selecting randomly according to the experimental design scheme; Stay complete root system, blot the moisture of root surface attachment with blotting paper after, install root chamber outer wall 1 with top cover 5 again and go back; Sealing and fixing is good, reconstitutes a complete root chamber.When sampling, through the through hole on the top cover 59, measure the indoor temperature of root, pressure respectively, and the intake-gas sample, utilize the gas Chromatographic Determination concentration of carbon dioxide, calculate the root breath amount.
Two, the simulated determination of hygrophyte root breath:
After assembling as requested and adjusting each locellus, plant in the plantation carries out preliminary treatment; When measuring,, connect the mist supply pipe of nitrogen, oxygen at first through the through hole 9 on the root chamber collet 6; Utilize the different anaerobic environment of different proportion simulation of these two kinds of gases; Then, mounting temperature meter, piezometer and sampler in the through hole 9 of top cover 5, intake-gas sample and relevant parameter; Utilize the gas Chromatographic Determination concentration of carbon dioxide, calculate the root breath amount.
Three, the application in soil nutrient heterogeneity and root distribution relation are studied:
After the requirement of experiment assembling and adjusting the space segmentation loose-leaf 3 of root chamber, divide the indoor different soil of nutrient density of inserting in difference respectively, in planting cup 2, plant seed or plant and cultivate; After pretreatment time reaches requirement of experiment, take off top cover 5 and outer wall 1, flushing earth obtains complete root system and divides indoor root in difference, supplies the parameter of aspects such as research soil nutrient and root length, root surface area, root branch, root biomass.
A kind of movable multipurpose root chamber; Comprise outer wall 1, planting cup 2, top cover 5, collet 6, central fixed shaft 7 and rubber seal 8; The top of outer wall 1 is provided with top cover 5, and the bottom is provided with collet 6, and inner center is provided with the lower end and is fixed on the central fixed shaft 7 on the collet 6;
Wherein: outer wall 1 is cylindric cylinder, and internal diameter 20cm, high 25cm, wall thickness 0.5cm are for the PVC material is processed;
Planting cup 2 internal diameter 5cm, dark 6cm in the cup, wall thickness 0.3cm also processes with the PVC material, and the bottom is the network structure of aperture 0.2cm, and the center is provided with the fixing hole that diameter is 0.5cm; The sealing rubber ring 8 of the high 6cm of planting cup 2 outer ring sets, thick 0.1cm;
Also be provided with 5 space segmentation loose-leaves 3 in the outer wall 1; Space segmentation loose-leaf 3 is the rectangular slab of high 25cm, wide 9.9cm, thick 0.3cm; Space segmentation loose-leaf 3 is vertically set on the collet 6, and a side is connected with outer wall 1, and the opposite side top cuts the bayonet socket of high 5.7cm, wide 2.5cm; The below of bayonet socket is provided with the sleeve pipe 12 that internal diameter is 0.5cm, thick 0.1cm, long 2.5cm, and sleeve pipe 12 positions on each space segmentation loose-leaf 3 all do not overlap; Be provided with central fixed shaft 7 in the sleeve pipe 12, central fixed shaft 7 is processed for the stainless steel rebar of external diameter 0.5cm, long 20.5cm, and the outside is provided with screw thread, and through nut space segmentation loose-leaf 3 and planting cup 2 is fixed on the collet 6;
Be provided with the circular hole of aperture 0.5cm in the upper and lower of space segmentation loose-leaf 3 respectively apart from limit portion 2cm place, be provided with the location sieve 4 of cutting apart loose-leaf 3 in order to fixed space in the circular hole, the external diameter of location sieve 4 is 0.2cm, and outer surface is provided with screw thread, and is furnished with nut; The wide 0.35cm of the equal ring set of side outside space segmentation loose-leaf 3 decannulations, the sealing rubber pad of thick 0.1cm.
In use, at first be installed to 5 space segmentation loose-leaves 3 on the central fixed shaft 7; On bayonet socket, place planting cup 2; Be through location sieve 4 on the space segmentation loose-leaf 3 with nut, adjust angle after, the nut on the stationary positioned sieve 4; Be installed in the bottom of centralized positioning axle 7 to collet 6, and be connected to the water pipe of external adjustment soil moisture in the through hole 9 on the collet 6; Seal seam everywhere again with commercially available Seal Oil; It is indoor to insert each minute of root chamber to ready planting soil according to requirement of experiment successively, and reaches 20 cm and be standard deeply; Sow seed or seedling in plantation cup 2 bottoms, then insert the thick soil of 1 cm again; After accomplishing above step, set up top cover 5 at last;
After accomplishing above-mentioned processing, can carry out the research of simulated determination and the soil nutrient heterogeneity and the root distribution relation of root breath mensuration, hygrophyte root breath, concrete steps are seen embodiment 1.
A kind of movable multipurpose root chamber; Comprise outer wall 1, planting cup 2, top cover 5, collet 6, central fixed shaft 7 and rubber seal 8; The top of outer wall 1 is provided with top cover 5, and the bottom is provided with collet 6, and inner center is provided with the lower end and is fixed on the central fixed shaft 7 on the collet 6;
Wherein: outer wall 1 is cylindric cylinder, and internal diameter 20cm, high 25cm, wall thickness 0.5cm are for the PVC material is processed;
Planting cup 2 internal diameter 5cm, dark 6cm in the cup, wall thickness 0.3cm also processes with the PVC material, and the bottom is the network structure of aperture 0.2cm, and the center is provided with the fixing hole that diameter is 0.5cm; The sealing rubber ring 8 of the high 6cm of planting cup 2 outer ring sets, thick 0.1cm;
Also be provided with 6 space segmentation loose-leaves 3 in the outer wall 1; Space segmentation loose-leaf 3 is the rectangular slab of high 25cm, wide 9.9cm, thick 0.3cm; Space segmentation loose-leaf 3 is vertically set on the collet 6, and a side is connected with outer wall 1, and the opposite side top cuts the bayonet socket of high 5.7cm, wide 2.5cm; The below of bayonet socket is provided with the sleeve pipe 12 that internal diameter is 0.5cm, thick 0.1cm, long 2cm, and sleeve pipe 12 positions on each space segmentation loose-leaf 3 all do not overlap; Be provided with central fixed shaft 7 in the sleeve pipe 12, central fixed shaft 7 is processed for the stainless steel rebar of external diameter 0.5cm, long 20.5cm, and the outside is provided with screw thread, and through nut space segmentation loose-leaf 3 and planting cup 2 is fixed on the collet 6;
Be provided with the circular hole of aperture 0.5cm in the upper and lower of space segmentation loose-leaf 3 respectively apart from limit portion 2cm place, be provided with the location sieve 4 of cutting apart loose-leaf 3 in order to fixed space in the circular hole, the external diameter of location sieve 4 is 0.2cm, and outer surface is provided with screw thread, and is furnished with nut; The wide 0.35cm of the equal ring set of side outside space segmentation loose-leaf 3 decannulations, the sealing rubber pad of thick 0.1cm.
In use, at first be installed to 6 space segmentation loose-leaves 3 on the central fixed shaft 7; On bayonet socket, place planting cup 2; Be through location sieve 4 on the space segmentation loose-leaf 3 with nut, adjust angle after, the nut on the stationary positioned sieve 4; Be installed in the bottom of centralized positioning axle 7 to collet 6, and be connected to the water pipe of external adjustment soil moisture in the through hole 9 on the collet 6; Seal seam everywhere again with commercially available Seal Oil; It is indoor to insert each minute of root chamber to ready planting soil according to requirement of experiment successively, and reaches 20 cm and be standard deeply; Sow seed or seedling in plantation cup 2 bottoms, then insert the thick soil of 1 cm again; After accomplishing above step, set up top cover 5 at last;
After accomplishing above-mentioned processing, can carry out the research of simulated determination and the soil nutrient heterogeneity and the root distribution relation of root breath mensuration, hygrophyte root breath, concrete steps are seen embodiment 1.
Claims (6)
1. movable multipurpose root chamber; Comprise outer wall (1), planting cup (2), top cover (5), collet (6), central fixed shaft (7) and rubber seal (8); The top of outer wall cylindraceous (1) is provided with top cover (5), and the bottom is provided with collet (6), and the inner center of outer wall (1) is provided with the lower end and is fixed on the central fixed shaft (7) on the collet (6); It is characterized in that: also be provided with at least three space segmentation loose-leaves (3) in the outer wall (1); The height of space segmentation loose-leaf (3) equates that with outer wall (1) space segmentation loose-leaf (3) is vertically set on the collet (6), and the outside of space segmentation loose-leaf (3) and outer wall (1) join; The inboard sleeve pipe (12) that passes through is connected with central fixed shaft (7); The inboard top of space segmentation loose-leaf (3) is provided with L shaped bayonet socket, and planting cup (2) is arranged on the bayonet socket, and the cup end of planting cup (2) is a network structure; The Center-to-Center fixed axis (7) at the cup end connects, and is provided with rubber seal (8) between the outer wall of planting cup (2) and the bayonet socket;
Described top cover (5) and collet (6) are disc structure, are equipped with through hole (9) on its surface, and the center of top cover (5) also is provided with the hole of placing planting cup (2).
2. a kind of movable multipurpose root according to claim 1 chamber, it is characterized in that: the quantity of described space segmentation loose-leaf (3) is 4 ~ 6, and the sleeve position on all space segmentation loose-leaves (3) does not all overlap.
3. a kind of movable multipurpose root according to claim 1 and 2 chamber; It is characterized in that: the upper and lower at space segmentation loose-leaf (3) is respectively equipped with circular hole; Be provided with the location sieve (4) that is used for fixing space segmentation loose-leaf (3) in the circular hole; The outer surface of location sieve (4) is provided with screw thread, and is furnished with nut.
4. a kind of movable multipurpose root according to claim 1 and 2 chamber is characterized in that: be equipped with sealing rubber pad between space segmentation loose-leaf (3) and outer wall (1), top cover (5) and the collet (6).
5. a kind of movable multipurpose root according to claim 1 chamber, it is characterized in that: described central fixed shaft (7) is provided with screw thread, and is furnished with nut.
6. a kind of movable multipurpose root according to claim 1 chamber, it is characterized in that: described collet (6) is provided with back-up ring (10), and the outer rim of back-up ring (10) overlaps with the outer rim of collet (6); The bottom in collet (6) outside also is provided with base (11).
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| CN106489574A (en) * | 2016-10-20 | 2017-03-15 | 中国科学院东北地理与农业生态研究所 | A kind of apparatus and using method for Fen Gen areas potted plant experiment |
| CN110487585B (en) * | 2019-09-20 | 2024-03-22 | 宁波市鄞州区农业技术推广站 | Rhizosphere sampling device for continuous cropping soil disease mechanism research and application method |
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| CN101502220A (en) * | 2009-03-06 | 2009-08-12 | 中国科学院东北地理与农业生态研究所 | Crop solid-liquid split-root cultivation device |
| CN102124933A (en) * | 2010-12-02 | 2011-07-20 | 北京市农林科学院 | Method for researching partial root drying irrigation technology of woody fruit trees |
| CN202285684U (en) * | 2011-11-09 | 2012-07-04 | 河南科技大学 | Movable type multipurpose root chambers |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502220A (en) * | 2009-03-06 | 2009-08-12 | 中国科学院东北地理与农业生态研究所 | Crop solid-liquid split-root cultivation device |
| CN102124933A (en) * | 2010-12-02 | 2011-07-20 | 北京市农林科学院 | Method for researching partial root drying irrigation technology of woody fruit trees |
| CN202285684U (en) * | 2011-11-09 | 2012-07-04 | 河南科技大学 | Movable type multipurpose root chambers |
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| CO2浓度增加和不同氮肥水平对冬小麦根系呼吸及生物量的影响;寇太记等;《植物生态学报》;20080430;第32卷(第4期);第922-931页 * |
| Transport and Distribution of 14CO2 Assimilate in lolium perenne in Response to Varying Nitrogen Supply to Halves of a Divided Root System;A.L.Barta;《Physiologia Plantarum》;19760930;第38卷(第1期);第48-52页 * |
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