CN102389030B

CN102389030B - Purpose of bacillus subtilis glycopeptide 168

Info

Publication number: CN102389030B
Application number: CN2011102748155A
Authority: CN
Inventors: 李叶青
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-09-16
Filing date: 2011-09-16
Publication date: 2013-12-25
Anticipated expiration: 2031-09-16
Also published as: CN102389030A

Abstract

The invention discloses a purpose of bacillus subtilis glycopeptide 168. The bacillus subtilis glycopeptide 168 is used in the livestock raising process, added antibiotics for livestock raising in the prior art are replaced or partially replaced, and the harm to the livestock body caused by the antibiotics is reduced. The bacillus subtilis glycopeptide 168 can realize the health care effects of enhancing the immunity of the livestock and eliminating various diseases generated in the livestock raising process, the livestock growth is promoted, the feed utilization rate is improved, in addition, no residue can be left in the livestock body, and no side effect can be caused on human bodies. The bacillus subtilis glycopeptide 168 solves the problem of side effect on the human bodies caused by residue of the antibiotics used in the livestock raising process in the livestock body, so the food safety is perfectly ensured.

Description

The purposes of Sublancin 168

Technical field

The present invention relates to the penkeeping additive, particularly the purposes of Sublancin 168 in penkeeping.

Background technology

(patent No. US 7 for american documentation literature, 358,076 B2) a kind of antibacterial peptide of being given birth to by producing bacillus subtilis is disclosed, called after Sublancin 168 is (in present patent application, the Chinese unification is referred to as " Sublancin 168 "), its aminoacid sequence following [Xaa is a dehydrogenated Thr (Dhb)]:

Gly Leu Gly Lys Ala Gln Cys Ala Ala Leu Trp Leu Gln Cys Ala Xaa

1 5 10 15

Gly Gly Xaa Ile Gly Cys Gly Gly Gly Ala Val Ala Cys Gln Asn Tyr

20 25 30

Arg Gln Phe Cys Arg

35

Have thioether and disulfide bond in Sublancin 168, its structural formula as shown in Figure 1.

Sublancin 168 can sterilize or bacteria growing inhibiting, there is good inhibitory action for gram positive bacteria, can also be used from sterilization or bacteria growing inhibiting with lantibiotics and other known antibiotic one, it can be for food preservation; Do not point out or provide except other any purposes for food preservation.

And in the prior art, often need in feedstuff and/or diet, add some additives in the penkeeping process, as antibiotic.Its purpose can strengthen domestic animal enhancing immunity, anti-stress and purifying aquaculture environment on the one hand, plays the health-care effect to domestic animal; Can treat the disease that domestic animal occurs in breeding process on the other hand, such as the domestic animal in the plant occurs that the mental status is poor, the phenomenon of having loose bowels such as loose and watery stool, soft while discharging material sample feces etc., can be thrown in antibiotic to address this problem in plant; The 3rd, the domestic animal growing environment needs frequent spraying medicine to carry out disinfection, so that domestic animal grows in a clean environment.

The additive for health care as domestic animal, exist that health-care effect is not obvious, the enhancing degree of domestic animal immunity is limited in actual applications, can not effectively shorten the shortcomings such as growth cycle of domestic animal.As the additive (now as veterinary drug) used for the treatment of livestock disease, such as antibiotic, tend to have in the domestic animal body certain residual.Antibiotic and residue thereof not only present harm to the domestic animal body, the more important thing is the mankind's these domestic animal meat and then harmful to human that contain antibiotic residues of eating food.Drug resistance problem appearance due to long-term and irregular interpolation antibiotic and then generation, cause antibiotic of the prior art can not effectively solve the disease occurred in the penkeeping process, often recurrence after medication.

Summary of the invention

For the deficiencies in the prior art, the object of the present invention is to provide the purposes of a kind of Sublancin 168 in penkeeping, replace or partly replace the domestic animal antibiotic used of feeding at present, reduce the harm of antibiotic to the domestic animal body, make and can play to domestic animal the health-care effect of enhancing immunity, can eliminate the numerous disease that domestic animal occurs in feeding process again, promote the domestic animal growth, improve efficiency of feed utilization, and in the domestic animal body, do not have residual, and then solve the antibiotic remains that uses in the penkeeping process in the domestic animal body and the negative effect that human body is caused, thereby guarantee well food safety.

Technical scheme of the present invention is achieved in that the purposes of Sublancin 168, in the penkeeping process, uses Sublancin 168.

The purposes of above-mentioned Sublancin 168, Sublancin 168 is added in animal feeding-stuff as feed additive.

The purposes of above-mentioned Sublancin 168, Sublancin 168 is added in the diet of domestic animal as the diet additive.

The purposes of above-mentioned Sublancin 168, the weight ratio of Sublancin 168 in diet is greater than zero, is less than or equal to one thousandth.

The purposes of above-mentioned Sublancin 168, Sublancin 168 is used as the domestic animal medication.

The purposes of above-mentioned Sublancin 168, directly give the domestic animal feeding, spray or be sprayed on the growing environment of domestic animal to domestic animal.

The purposes of above-mentioned Sublancin 168, Sublancin 168, for suppressing to kill and wound staphylococcus aureus, is treated mammitis of cow.

The purposes of above-mentioned Sublancin 168, Sublancin 168 is used for the treatment of sweet Borrow's disease.

The purposes of above-mentioned Sublancin 168, Sublancin 168 is used for the treatment of domestic animal and has loose bowels.

In the present invention, " feedstuff " refers to and directly do not supply with the livestock edible material that domestic animal is used, also needs to add other material before livestock edible; " diet " refers to directly edible material of domestic animal, adds the material mixture afterwards such as water as feedstuff.

The domestic animal of indication in the present invention, comprise poultry, domestic animal, as cattle, sheep, pig, chicken, dog etc.; Also comprise artificial breeding's aquatic animal, as fish, shrimp etc.

The invention has the beneficial effects as follows: Sublancin 168 is used for to penkeeping, it is as feed additive or be added in the diet of domestic animal, can play to domestic animal the health-care effect of enhancing immunity, eliminate the numerous disease that domestic animal occurs in feeding process, promote the domestic animal growth, improve efficiency of feed utilization; It uses as the domestic animal medication, can eliminate the numerous disease that domestic animal occurs in feeding process, especially can treat the domestic animal phenomenon of having loose bowels, and suppress and kill staphylococcus aureus, treatment mammitis of cow, and do not have residually in the domestic animal body, more can not cause any negative effect to human body; Thoroughly solve the antibiotic remains that uses in the penkeeping process in the domestic animal body and the negative effect that human body is caused, thereby guaranteed well food safety.

The accompanying drawing explanation

The structural formula that Fig. 1 is Sublancin 168.

The specific embodiment

First is used Sublancin 168 in the penkeeping process, Sublancin 168, as feedstuff and/or diet additive, plays the health-care effect that improves immunity or treats the disease (as having loose bowels) occurred in the penkeeping process domestic animal:

Embodiment 1

The A chicken farm, chicken farm cultivation occurs that when feedstuff is changed food-intake reduces, and chicken spirit is in a trance, and active not when feed and activity, the feces compared with normal is soft, and the tendency of having loose bowels is arranged.If now with antibiotic, treat the appearance that is easy to cause its intestinal tract disease, and the medication later stage also can concurrent coccidiosis.In addition, due to the appearance of gastroenteropathy and then cause its function of intestinal canal not normal, can not catapepsis after feed, cause some chickens to occur drawing and expect just phenomenon.

As domestic animal health care, somatotrophic additive, in the chicken diet, add Sublancin 168.The weight ratio of Sublancin 168 in diet is 5/10000000ths to 6/10000000ths, and additive capacity successively decreases in time, continues to feed to use about 10 days.

With adding the antibiotic group, compare, the chicken after the Sublancin 168 has been added in feed, and its food-intake obviously increases, and more active when walking about and taking food.Intestinal tract disease does not also occur during this time, and feces granularity and the hardness of chicken are normal.Chicken group's gastrointestinal function normal level, can absorb after feed in catapepsis, expects that just phenomenon significantly reduces.With adding the antibiotic group, compare, coccidiosis does not occur in the chicken group later stage that feeding has added Sublancin 168 yet, and feedstuff-meat ratio reduces, and then has saved aquaculture cost.

Embodiment 2

The B pig farm, sow one week puerperal the left and right food-intake do not return to yet normal level in the past, suckling piglet had the diarrhoea phenomenon 3 days puerperal.Utilize antibiosis usually as sow health care and the nursing medicine in puerperal, DeGrain.

Health product based on sow prevention and treatment are used, and use Sublancin 168 in diet.Directly add Sublancin 168 in the diet of sow, the weight ratio of Sublancin 168 in diet is 8/10000000ths to 10/10000000ths.Feed and use about 10 days continuously.

The food-intake of sow returns to normal level at medicine feed about 3 days, and every physical signs also returns to normal range, has made sow reduce and has infected the probability of other diseases.The symptom of having loose bowels of piglets also progressively takes a turn for the better and disappears, and body weight also progressively increases.

Second portion is used Sublancin 168 in the penkeeping process, and Sublancin 168 is used for the treatment of mammitis of cow:

Embodiment 3

Mastitis is the common and high morbidity of milch cow.For the milch cow that suffers from mastitis, Therapeutic Method commonly used is the conventional antibiotic of application.Remove antibiotic drug resistance produce make therapeutic effect not ideal outside, the more important thing is in therapeutic process and the following period of time after treatment need to be abandoned milk, and then the economic loss produced is apparent.

C milk cattle cultivating family, for several the milch cows that suffer from mastitis, used Sublancin 168 to smear affected part, recovery from illness after a week.Show that Sublancin 168 can effectively kill its pathogenic bacterium staphylococcus aureus.

Third part is used Sublancin 168 in the penkeeping process, and Sublancin 168 carries out disinfection to the domestic animal growing environment as surface disinfectant:

Embodiment 4

The D chicken farm, used Sublancin 168 to spray the chicken growing environment, and flushing liquor concentration is 1ppm, within every three days, sprays once.With respect to the chicken growing environment that does not spray Sublancin 168, the chicken prevalence reduces by 50%.

Embodiment 5

The E pig farm, used Sublancin 168 to spray the pig growing environment, and flushing liquor concentration is 1ppm, and spray once every day.With respect to the pig growing environment that does not spray Sublancin 168, the pig prevalence reduces by 60%.

It needs to be noted: the weight ratio of Sublancin 168 in diet can, for being greater than zero, being less than or equal to any number between one thousandth, all can realize goal of the invention of the present invention, but the weight ratio of Sublancin 168 in diet is less, the effect relative mistake of bacteriostasis and sterilization some, the weight ratio of Sublancin 168 in diet is larger, although the effect of bacteriostasis and sterilization is better, the production and processing difficulty increases, and production and processing cost and user cost also can correspondingly increase, preferably the weight ratio of Sublancin 168 in diet is for being greater than 1/10000000th, be less than or equal to 100/10000000ths, further the preferred weight ratio of Sublancin 168 in diet is for being greater than 1/10000000th, be less than or equal to 20/10000000ths, can either play to domestic animal the health-care effect of enhancing immunity like this, it uses as the domestic animal medication, can eliminate the numerous disease that domestic animal occurs in feeding process again, especially can treat the domestic animal phenomenon of having loose bowels, and suppress and kill staphylococcus aureus, the treatment mammitis of cow, and be easy in practice production and processing, save production cost, alleviate raiser's financial burden.

Claims

1. the application of Sublancin 168 in preparing feed additive.

2. application as claimed in claim 1, the weight ratio of described Sublancin 168 in feedstuff is greater than zero, is less than or equal to one thousandth.