CN102388881B - Seed coating agent containing chitosan oligosaccharide as well as use and use method thereof - Google Patents
Seed coating agent containing chitosan oligosaccharide as well as use and use method thereof Download PDFInfo
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- CN102388881B CN102388881B CN201110333573.2A CN201110333573A CN102388881B CN 102388881 B CN102388881 B CN 102388881B CN 201110333573 A CN201110333573 A CN 201110333573A CN 102388881 B CN102388881 B CN 102388881B
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- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 32
- 239000011248 coating agent Substances 0.000 title abstract 5
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 241000238631 Hexapoda Species 0.000 claims abstract description 4
- 238000002791 soaking Methods 0.000 claims description 7
- 230000007226 seed germination Effects 0.000 claims description 6
- 238000007598 dipping method Methods 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 241000607479 Yersinia pestis Species 0.000 claims description 2
- 230000035784 germination Effects 0.000 abstract description 9
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 description 34
- 238000006116 polymerization reaction Methods 0.000 description 8
- 230000015556 catabolic process Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 229920001661 Chitosan Polymers 0.000 description 5
- 150000002482 oligosaccharides Polymers 0.000 description 5
- 229920000642 polymer Polymers 0.000 description 5
- 229920002101 Chitin Polymers 0.000 description 4
- 240000000249 Morus alba Species 0.000 description 4
- 235000008708 Morus alba Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 229920001542 oligosaccharide Polymers 0.000 description 4
- 239000004925 Acrylic resin Substances 0.000 description 3
- 229920000178 Acrylic resin Polymers 0.000 description 3
- 240000008067 Cucumis sativus Species 0.000 description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 229960002442 glucosamine Drugs 0.000 description 3
- MSWZFWKMSRAUBD-IVMDWMLBSA-N glucosamine group Chemical group OC1[C@H](N)[C@@H](O)[C@H](O)[C@H](O1)CO MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 235000002566 Capsicum Nutrition 0.000 description 2
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 2
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 2
- 239000005018 casein Substances 0.000 description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 2
- 235000021240 caseins Nutrition 0.000 description 2
- 238000002144 chemical decomposition reaction Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 125000003147 glycosyl group Chemical group 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229920002857 polybutadiene Polymers 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 239000004814 polyurethane Substances 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000006196 deacetylation Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- YZHUMGUJCQRKBT-UHFFFAOYSA-M sodium chlorate Chemical compound [Na+].[O-]Cl(=O)=O YZHUMGUJCQRKBT-UHFFFAOYSA-M 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a seed coating agent containing chitosan oligosaccharide as well as the use and use method thereof, which is characterized in that the weight percentage of chitosan oligosaccharide in a composition is 0.008% to 0.024%. The invention further provides the seed coating agent containing chitosan oligosaccharide, which is characterized in that the weight percentage of chitosan oligosaccharide in the seed coating agent is 0.008% to 0.024%. After seeds are processed by the seed coating agent provided by the invention, not only can the germination ratio of seeds be improved, but also the insect and disease resistance and the stress tolerance of the crop can be improved after the seeds of the crop germinate.
Description
Technical field
The invention belongs to agrochemical field, especially relate to the application of a kind of chitosan oligosaccharide in preparation seed coat agent, be specifically related to a kind of seed coat agent comprising chitosan oligosaccharide and uses thereof and using method.
Background technology
In current research, have the research of chitosan oligosaccharide process seed, such as Guo Wei China etc., chitosan oligosaccharide is on the impact of germination of cucumber seeds and growth of seedling and photosynthesis characteristics, China's agronomy circular 2009,25 (03): 164-169, find that the degree of polymerization can promote the germination rate of crop at the chitosan oligosaccharide of 3-10 under study for action.Also useful chitosan oligomer solution-treated Mulberry Seeds and foliage-spray is carried out to mulberry tree, result shows: the oligopolymerization chitosan sugar solution treatment Mulberry Seeds of 0.5%, the germination rate of Mulberry Seeds can be improved, also have, with mean molecule quantity be 1500, concentration is that the facilitation effect of the chitosan oligomer of 0.2% is best, can improve the germination vigor of wheat seed, germination rate.
Also has the research for chitin oligo saccharide process seed, its research shows in 0.2-1.0mg/ml concentration range, the process of chitin oligo saccharide solution seed soaking can significantly improve Tobacco Seed Germination rate and germination vigor, optimum concentration is 0.4mg/ml, is significantly higher than contrast by tobacco seed daughter endoperoxide enzyme (POD) activity of 0.4mg/ml chitin oligo saccharide process.
Present invention finds the chitosan oligosaccharide composition that can be used in preparing seed coat agent, said composition, in using as seed coat agent, has specific effect.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of new purposes of chitosan oligosaccharide, particularly, the invention provides the purposes that a kind of composition comprising chitosan oligosaccharide is used as seed coat agent, it is characterized in that the weight percent content that chitosan oligosaccharide accounts for composition is 0.008-0.024%, preferably, be 0.01-0.02%
Particularly, present invention also offers a kind of seed coat agent comprising chitosan oligosaccharide, it is characterized in that the percentage that chitosan oligosaccharide in this seed coat agent accounts for seed coat agent weight is 0.008-0.024%, preferably, is 0.01-0.02%.
In above-mentioned seed coat agent, can comprise adhesive or film forming agent, wherein said adhesive is preferably sodium alginate, casein sodium, alphalise starch, sodium carboxymethylcellulose etc.Film forming agent is preferably albumen film forming agent, acrylic resin film forming agent, butadiene resin film forming agent, polyurethane finishing agent, cellulose nitrate film forming agent etc., or be selected from the kind of mutual modification, as the agent of acrylic resin modification casein filming, acrylic resin polyurethane copolymerization resin, polyethylene, acrylate modified butadiene resin, polyurethane-modified cellulose nitrate film forming agent etc.The adhesive no matter added in seed coat agent or the amount of film forming agent are how many, and the content of chitosan oligosaccharide in seed coat agent all will be kept to remain unchanged.
In above-mentioned seed coat agent, can be included as and make seed coat agent obtain other effect and other the chemical agent added, such as insecticide, bactericide, weed killer herbicide, plant growth regulator or fertilizer etc., the amount of other the chemical substance no matter added is how many, generally, ensure that the content of chitosan oligosaccharide in seed coat agent remains unchanged.
Present invention also offers comprise chitosan oligosaccharide seed coat agent for the treatment of the method for seed, it is characterized in that the weight content of chitosan oligosaccharide in seed coat agent is 0.008-0.024%, preferably, be 0.01-0.02%, the treating method comprises but be not limited to dressing, seed dressing, dipping, seed soaking or vexed kind.Wherein dressing refers to outer surface seed coat agent being coated in seed, becomes one or more layers the different thickness, the different flexible Multifunctional protective sheath that are adhered tightly to surface after making its drying, and seed dressing refers to and medicinal powder or liquid mixed with seed; Dipping is medicine liquid spray on seed, and constantly stirs seed is all soaked, and covers the coverings such as mat, piles vexed rear sowing; Seed soaking refers to that a seed or seedling are immersed in certain density liquid, makes seed or seedling absorb medicament through certain hour; Vexed kind refers to the method for liquid and seed being mixed the vexed certain hour of rear heap and sowing.
Present invention also offers a kind of purposes comprising the seed coat agent of chitosan oligosaccharide, it is characterized in that by seed coat agent seed treatment, cold-resistant, the drought-resistant ability that improve the seed germination rate of crop, strengthen the diseases and insect pests resistance of crop, strengthen crop, wherein chitosan oligosaccharide accounts for the percentage composition of seed coat agent weight is 0.008-0.024%, preferably, be 0.01-0.02%.
In the present invention, chitosan oligosaccharide is Glucosamine is the mixture of the high molecular polymer of 2-30 by the degree of polymerization that Isosorbide-5-Nitrae-glycosidic bond is formed by connecting, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be prepared by any means, such as following method: the shitosan of deacetylation more than 70% is the mixture of the chitosan oligomer of 2-30 by the degree of polymerization that the methods such as physical degradation methods, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Or, obtain chitin by extracting from fungal cell wall and be the mixture of the chitosan oligomer of 2-30 by the degree of polymerization that the methods such as physical degradation methods, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Or, by by Glucosamine by the degree of polymerization that Isosorbide-5-Nitrae-glycosidic bond chemosynthesis is polymerized being the mixture of the polymer of 2-30.Current chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer, be not that is the oligosaccharides form of the single degree of polymerization in chitosan oligosaccharide, but the chitosan oligosaccharide monose of often kind of degree of polymerization all may exist.So no matter by which kind of method prepared; or by the commercially available prod that above-mentioned preparation method prepares; as long as it is that Glucosamine is by 1; the mixture that the degree of polymerization that 4-glycosidic bond is formed by connecting is 2-30, mean molecule quantity is the daltonian high molecular polymer of 1000-3000 is exactly chitosan oligosaccharide of the present invention; it can contain acetyl group; but the sugar unit of bonding acetyl group is no more than the amount of 40%, preferably more than the amount of 30%, especially preferably more than 20% amount.Chitosan oligosaccharide commercially available is at present all chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth substantially.
Beneficial effect of the present invention:
Present invention finds the seed coat agent comprising chitosan oligosaccharide.After this seed coat agent process seed, can not only seed germination rate be improved, but also disease and insect resistance, the anti-adversity ability of seed germination ensuing crop can be improved.And the present invention contributes especially and is also to have found to apply the good mode and the method that comprise the seed coat agent process seed of chitosan oligosaccharide by research.
Embodiment
In order to understand the present invention, further illustrate the present invention with embodiment below, but do not limit the present invention.
Embodiment 1:
The cucumber seeds choosing full seed uses chitosan oligosaccharide (5%) and the distilled water seed soaking of different extension rate respectively, soaks 24h in 25 DEG C of insulating boxs.Each process 30, repeats for 3 times.Then use distilled water flushing 3 times, be placed in the culture dish being covered with 3 metafiltration paper, add 10ml distilled water, sprout 5 days under dark surrounds in 25 DEG C of insulating boxs, statistical survey root length and plant height.
The impact that table 1 variable concentrations oligosaccharide seed soaking grows corn root
As can be seen from Table 1, the germination rate of chitosan oligosaccharide solution seed soaking to cucumber has promotion crop, and can promote the growth of crop at the early growth period of crop.
Embodiment 2:
Soak seed 5 hours to capsicum with the chitosan oligosaccharide solution of variable concentrations, be put in by seed afterwards and be lined with in the culture dish of filter paper, each culture dish 50 seeds, add up its chitting piece number every day, totally 9 process, each process repetition 3 times, and concrete outcome is as follows:
Table 2 chitosan oligosaccharide variable concentrations is to the effect of pepper seed germination
As can be seen from the above table, application chitosan oligosaccharide process seed, can improve the germination rate of seed.
Claims (4)
1. the composition comprising chitosan oligosaccharide is used as a purposes for seed coat agent, it is characterized in that chitosan oligosaccharide accounts for the weight percent content 0.00083%, 0.00166%, 0.0025%, 0.005% or 0.0005% of composition.
2. comprise a seed coat agent for chitosan oligosaccharide, it is characterized in that the percentage that chitosan oligosaccharide in this seed coat agent accounts for seed coat agent weight is 0.00083%, 0.00166%, 0.0025%, 0.005% or 0.0005%.
3. comprise the method for seed coat agent for the treatment of seed of chitosan oligosaccharide, it is characterized in that the weight content of chitosan oligosaccharide in seed coat agent is 0.00083%, 0.00166%, 0.0025%, 0.005% or 0.0005%, the treating method comprises but be not limited to dressing, seed dressing, dipping, seed soaking or vexed kind.
4. one kind comprises the purposes of the seed coat agent of chitosan oligosaccharide, it is characterized in that by seed coat agent seed treatment, cold-resistant, the drought-resistant ability that improve the seed germination rate of crop, strengthen the diseases and insect pests resistance of crop, strengthen crop, wherein chitosan oligosaccharide accounts for the percentage composition of seed coat agent weight is 0.00083%, 0.00166%, 0.0025%, 0.005% or 0.0005%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110333573.2A CN102388881B (en) | 2011-10-28 | 2011-10-28 | Seed coating agent containing chitosan oligosaccharide as well as use and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110333573.2A CN102388881B (en) | 2011-10-28 | 2011-10-28 | Seed coating agent containing chitosan oligosaccharide as well as use and use method thereof |
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| CN102388881B true CN102388881B (en) | 2015-03-18 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104585169B (en) * | 2014-12-22 | 2016-08-24 | 党永富 | Wheat seed coating agent safe additive, preparation method and application |
| CN104926534A (en) * | 2015-06-29 | 2015-09-23 | 山东卫康生物医药科技有限公司 | High-purity chito-oligosaccharide controlled release fertilizer based on separation techniques and preparing method thereof |
| CN105052933A (en) * | 2015-07-29 | 2015-11-18 | 山东亿嘉农化有限公司 | Efficient seed coating agent, preparation method and applications thereof, and method using same to process seeds |
| CN105272598A (en) * | 2015-10-23 | 2016-01-27 | 金正大生态工程集团股份有限公司 | Wheat seed dressing agent and preparation method thereof |
| CN110214781A (en) * | 2019-07-02 | 2019-09-10 | 赵士明 | A kind of amino-oligosaccharide film forming agent pesticide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455208A (en) * | 2008-10-24 | 2009-06-17 | 北京易众恒科技有限公司 | Seed treatment agent and preparation technique thereof |
| CN102283201A (en) * | 2011-09-28 | 2011-12-21 | 扬州日兴生物科技股份有限公司 | Chitin oligosaccharide plant growth regulator and application thereof in agriculture |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455208A (en) * | 2008-10-24 | 2009-06-17 | 北京易众恒科技有限公司 | Seed treatment agent and preparation technique thereof |
| CN102283201A (en) * | 2011-09-28 | 2011-12-21 | 扬州日兴生物科技股份有限公司 | Chitin oligosaccharide plant growth regulator and application thereof in agriculture |
Non-Patent Citations (1)
| Title |
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| 壳寡糖油菜种衣剂剂型应用效果研究;陆引罡等;《种子》;20031231(第4期);38-39（92） * |
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Denomination of invention: Seed coating agent containing chitosan oligosaccharide as well as use and use method thereof Effective date of registration: 20160822 Granted publication date: 20150318 Pledgee: Bank of Hainan Limited by Share Ltd. Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2016460000002 |
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Address after: Fuchang Village, Laocheng Development Zone, Chengmai County, Hainan Province, 571923 Patentee after: Hainan Zhengye Biotechnology Co.,Ltd. Address before: 571924 Fuchang village, Laocheng Development Zone, Chengmai County, Hainan Province Patentee before: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. |