CN102379238A - Multi-ecological-area recurrent mixed selection - mixed planting cotton breeding method - Google Patents
Multi-ecological-area recurrent mixed selection - mixed planting cotton breeding method Download PDFInfo
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Abstract
The invention relates to the field of breeding and specifically relates to a multi-ecological-area recurrent mixed selection - mixed planting cotton breeding method, which comprises the following steps of: 1) preparing cotton hybridized combinations; 2) reproducing generation F1 hybrid seeds in the south for increasing the generation; 3) respectively sowing generation F2 seeds in cotton regions of the Yellow River Valley, the Yangtze River Valley and the northwest inland; 4) selecting generation F3 seeds of excellent individual plants and reproducing in the south for increasing the generation; 5) respectively sowing generation F4 seeds in the cotton regions of the Yellow River Valley, the Yangtze River Valley and the northwest inland; and 6) selecting generation F6 seeds of excellent individual plants, sowing the seeds in hybrid seed production fields and selecting excellent individual plants according to breeding purposes. The method provided by the invention complements a modified backcrossing and recurrent selection method and improves the yield, the quality and the insect resistance. The method has the advantages of high breeding efficiency, diversified varieties, high adaptability, high purity and good consistency, and lays a foundation for breeding better varieties in future.
Description
Technical field
The present invention relates to the breeding field, particularly, the present invention relates to many ecotope wheel back-mixing choosing-miscegenation cotton breeding methods.
Background technology
China is that the world mainly produces cotton big country, and gross yield accounts for about 25% of world's output of cotton, ranks first place.Plant cotton with a long historyly, plantation was just arranged in south China before more than 2000 years.Vast in territory, from the east of Liaohe River Basin and Yangtze River Delta, to west, Tarim Basin edge; South is from the Yaxian, and Manas' river valley is supported in north, between 76 °～124 ° of 18 °～46 ° of north latitude, east longitude; In this broad territory, differ to the requirement of cotton variety in various places.For this reason; This is specialized in the national mechanism of cotton scientific research except The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute in China; All be provided with cotton breeding mechanism in relevant provincial and local level universities and colleges, scientific research institutions of planting cotton province (city, autonomous region, formation), this is in the regular period, particularly since the reform and opening-up; Cultivated a large amount of new varieties, played the impetus of long-pending level promoting Cotton in China production.In the last few years, the annual new cotton variety through country and provincial authorization of China surpassed 100, and cotton variety " many, random, assorted " phenomenon also is on the rise; In the Yangtze river basin and two big cotton growing areas, the Huanghe valley, most of cotton grower plants 2 above cotton varieties in oneself several mu of cotton fields, a village kind of some places up to a hundred kinds; Mix to receive to mix and sell; Cotton fiber length is inconsistent, and quality descends, and textile industry is caused adverse effect.Its main cause is, it is relatively poor to environment, climate adaptability mostly to count cotton varieties at present, and the cotton grower plants single variety and has a big risk, and its basic reason is the limitation of breeding technique method.
The pedigree back-and-forth method once played great role at the early stage breeding phase of cotton.The kind that China's twentieth century sixties and the beginning of the seventies are bred is wherein most ofly educated with this method, like Xuzhou 209, Xuzhou 1818, No. 2, middle cotton institute, No. 3, middle cotton institute, Shanghai cotton 204 etc.The pedigree back-and-forth method is utilize to produce to go up the natural variation that takes place in the varietal population of large-area applications or residual genetic variation, selects elite seedling wherein, cultivates into new varieties.Its probability is extremely low, but in case select after, just improved variety faster.After the eighties, the cotton pedigree selects breeding not obtain big progress, and its reason possibly be because the cotton field uses pesticides indiscriminately, and has eliminated a large amount of pollination medium insects, thereby has reduced the result of outcrossing rate.
Mutation breeding successful example on cotton is few.Biotechnology such as the genetic transformation of exogenous Bt Insect Resistant Cotton make substantial progress in the initiative of cotton new material; Also have molecular marking technique also to become focus in recent years; But maximum and the most general method or the crossbreeding of cotton breeding utilization now, biotechnology must combine with crossbreeding just can breed improved seeds.Backcross method derives from from crossbreeding, and is obvious to improving the single traits effect.Distant hybridization is also derived from from crossbreeding.Because affiliation is distant between the parent, on breeding practice, there are two hang-ups, the one, parent's incompatibility; The 2nd, offspring's sterility.
Cotton breeding is no matter adopt any syntax, and it roughly is three steps that the procedure of breeding is concluded: find or create variation, select variation, stable variation.New variation is the breeding prerequisite.So, in the past some scholars to the research of cotton breeding method also all wherein at this.As 1977, Meredith, Jr. once with the composite of three species hybrid genetic backgrounds as donor parents, upland cotton high yield range of goods Mount Tai word cotton 16 is as recurrent parent, carried out 3 and taken turns and backcross.Therefrom select lint yield combine with fibre strength offspring preferably, but backcross can not these two proterties of radical change between the basic genetic relation of negative correlation.And for example the Ma Jiazhang researcher of The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute is since nineteen eighty-two; The research cotton mixes and selects-mixes and hand over breeding system, takes the multi-parent strain multiple cross, and the continent is mixed choosing-Hainan and mixed friendship breeding method or the like; Impetus is all being created in the new variation, until there is not big variation now.China last hundred tame cotton breeding mechanisms (other crop is also similar) create new variation back and are just selecting variation a certainly at present, stable variation, and the kind limitation of cultivation is very strong, and is seldom suitable for large area applications.Be the reason of traffic and funds in the past, present powerful along with national power, the each side condition is all ripe, when should pay attention to creating new variation, also will strengthen selecting variation, stablizes Study on Variation.
Earlier 2000s, the cotton variety 33B that the cotton company of U.S.'s Mount Tai word cultivates gets into China, and large tracts of land is promoted rapidly, is not only because resistance to insects is good, and its purity height, high conformity, wide adaptability are crucial especially.
Therefore, at present in the cotton breeding field, the eurytopicity of newly breeding the cottonizing kind has much room for improvement.
Summary of the invention
Therefore, in order to address the above problem, inventor of the present invention proposes and accomplishes the present invention.
The purpose of this invention is to provide many ecotope wheel back-mixing choosing-miscegenation cotton breeding methods.
According to many ecotope wheel back-mixing choosing-miscegenation cotton breeding methods of the present invention, said method comprising the steps of:
1) hybrid seeding is carried out in preparation cotton male parent, hybridization of female parent combination;
2) F
1Add generation for the crossbreed Seed Propagation in Southern Winter;
3) F
2For seed divide kind in the Huanghe valley, the landlocked cotton region in the Yangtze river basin and northwest;
4) choose F
2The fine individual plant in generation mixes receives, i.e. F
3For seed, and south numerous added-generation;
5) F
4For seed divide kind in the Huanghe valley again, the landlocked cotton region in the Yangtze river basin and northwest;
6) choose F
4The fine individual plant in generation mixes receives, i.e. F
5For seed, and south numerous added-generation;
7) choose F
5The fine individual plant in generation mixes receives, i.e. F
6For seed; The plantation on hybrid seeding ground, and according to breeding objective selection fine individual plant, selected individual plant further carries out plant, strain system and variety comparative test; The offspring of selfing in continuous 2 years; Detect purity through comparative test and classification marking, breed through isolation simultaneously and guarantee purity, thereby seed selection obtains new cotton variety.
According to the method for the invention, in step 1), preparing hybrid combination technique key is that the parent selects, and consults parent's source earlier, identifies and screen parent's economical character and economic characters again.Generally annual preparing hybrid combination in June, according to breeding objective, two kinds of breeding material that general branch creation is new and seed selection new varieties.Selecting the principle of parent in line with complementation, mainly is source, output, quality and resistance aspect.Preparing hybrid makes up this hybridization of available parents, and (((A * B) * (whether C * D) etc. show decision according to the offspring and will backcross for A * B) * C or multi-parent strain for A * B), three parent's hybridization.
Particularly, many ecotope wheel back-mixing choosing-miscegenation cotton breeding methods according to the present invention can may further comprise the steps:
1, beginning hybrid seeding before and after annual July 15 finished before August 10, results before and after September 25, F
1Deliver to Sanya, Hainan at the beginning of 10 months generations and carry out the Seed Propagation in Southern Winter plantation,, suitably enlarge cultivated area according to the quantity in breeding base; Its objective is the temperature that utilizes Hainan in winter, quicken the variation of crossbreed, shorten breeding time; Results will suitably shift to an earlier date, generally before and after the March 20, rolled spend after; Seed should be mailed each ecological test site rapidly to, guarantees to sow on time.
2, F
2For seed divide kind in the Huanghe valley, the landlocked three big cotton regions in the Yangtze river basin and northwest; 2-3 point established in each cotton region; Each point will have the specificity of weather, soil and cropping pattern, like accumulated temperature: basic, normal, high, precipitation: many, few; Saline land, nonirrigated farmland, sick ground, the spring is cotton, wheat (rape) cover is cotton, wheat melon cotton-padded covering is cotton, wheat stubble is cotton or the like, because of F
2On behalf of segregation population, same hybrid combination can be selected numerous types of materials under different ecological condition.Each is combined in about 0.2 mu of every dibbling; Began screening on September 20; The final election combination of first round elder generation, general mortality are about 50%, and second takes turns that final election economical character and economic characters show individual plant preferably from selected combination; It is just passable that each individual plant chooses portion's bell to receive 1-2, its objective is acquisition genetic variation diversity as much as possible.The same combination seed of each pilot mixes, and delivers to Sanya, Hainan at the beginning of 10 months and carries out Seed Propagation in Southern Winter and add generation.
3, F
3For seed winter in Hainan cultivated area want corresponding expansion, except adding, also to make variation fully for the function, every combination mixes before and after March 20 and receives fine individual plant more than 0.2 mu, sends each ecological test site before April 10 to.
4, F
4For seed divide kind in the Huanghe valley again, the Yangtze river basin and northwest each ecological test site, landlocked three big cotton region, each is combined in every dibbling more than at least 0.3 mu, in order to enlarge colony.Begin screening same September 20; The first round also is first final election combination; General mortality is about 60%, and second takes turns that the final election economical character shows individual plant preferably with economic characters from selected combination, and each individual plant is chosen the bell receipts 1-2 of portion; The same combination seed of each pilot mixes, and delivers to Sanya, Hainan at the beginning of 10 months and carries out Seed Propagation in Southern Winter and add generation.
5, F
5For seed winter in Hainan cultivated area mix before and after March 20 and receive fine individual plant more than at least 0.3 mu, sent hybrid seeding ground (like Earthquake of Anyang station in Henan) before April 10 to.
6, F
6Be unified in hybrid seeding ground (like our department of Earthquake of Anyang station in Henan middle cotton institute) plantation before and after April 25 more than 1 mu for seed, its objective is that being beneficial to the breeder observes, identifies and screen the time of infertility.According to breeding objective, select individual plant, the selected individual plant in general every combination land for growing field crops is more than 200, and through indoor species test in winter, attribute test keeps economical character and all good individual plant of economic characters, F
6Basicly stable for individual plant, there is no need Seed Propagation in Southern Winter and add generation, moreover Nan Fanzhong also possibly produce unfavorable variation because of cross pollination, machinery and people for factor such as mixing.Selected individual plant further carries out plant, strain system and variety comparative test; All be located at hybrid seeding ground (like our department of Earthquake of Anyang station in Henan middle cotton institute); The kind of promoting with current large tracts of land is contrast; By kind or material requirements, identify its purity, uniformity and specificity on the one hand, identify its prematurity, output, quality, disease and insect resistance according to production requirement on the other hand.Special to a certain proterties, the comprehensive strain that can't use production is provided with the back as backbone parent and utilizes; Various proterties are all showed excellent strain, and plantation in 1 year is therefrom selected again individual plant more than 2 mu, through indoor species test in winter, and attribute test, the selfing in continuous 2 years of the individual plant of reservation is in order that kind stable and consistent more.
The offspring of selfing in continuous 2 years participates in institute's level variety comparative test, the evaluation of disease-resistant worm, SSR molecular labeling purity detecting on the one hand, further participation country or provincial regional test → production test → become kind through authorization.Isolate on the other hand and breed; Do not isolate, only plant the wide corn isolation strip of 2m, isolation 100m and isolation 500m through continuous 3 years and test, the purity of not isolating, only planting the wide corn isolation strip of 2m, isolation 100m and isolation 500m is respectively 87.6%, 93.1%, 96.5% and 99.9%.Show and to isolate the purity that just can guarantee kind more than the 500m.
According to many ecotope wheel back-mixing choosing-miscegenation cotton breeding methods of the present invention; Those of ordinary skills can choose excellent strain based on common practise, combination breeding objective; For example; Earlier from big Tanaka choose moderate growth duration, looser, growth potential is strong, medium blade is big or small, knot bell property strong (more than individual plant 20 bells), bigger, the disease-resistant worm of bell are good, the individual plant that is difficult for early ageing; Indoor species test in winter is measured bell weight, ginning outturn and seed and is referred to, gined cotton is delivered to cotton quality supervision and inspection center of the Ministry of Agriculture and carried out the fiber quality test.Last final election individual plant index is: Dan Lingchong >=5.5g, ginning outturn >=40%, seed refer to more than the 10g, length >=29mm, specific strength >=28.0cN/tex, mic value 3.7～5.2.
The invention provides a kind of method that in the new variation of creation, also adopts biotechnology to combine, also replenished modification property and backcrossed and the recurrent selection method, improve output, quality and resistance to insects with crossbreeding.Seed selection efficient is high, variety type is various, wide adaptability, purity height and uniformity, and the better kind of more later cultivation lays the foundation.
Description of drawings
Fig. 1 method flow diagram of the present invention.
Embodiment
Embodiment 1 middle cotton 44 seed selection processes
Middle cotton 44 parental combinations are (in 2369 * Tamcot CD3H) F
2* middle cotton institute 17.Wherein, in 2369 be the strain of this institute seed selection, main feature is that knot bell property is strong, concentrates; Tamcot CD3H is from the U.S., and disease and insect resistance is good; Middle cotton 17 are high yield, fine quality that the applicant breeds.
Nineteen ninety is 2369 * Tamcot CD3H in the preparing hybrid combination of our department of Earthquake of Anyang station in Henan middle cotton institute, and Seed Propagation in Southern Winter adds generation, and 1991 at the F of our department of Earthquake of Anyang station in Henan middle cotton institute
2Generation choosing high yield, individual plant and middle cotton institute 17 disease-resistant, that salt-resistance is good do hybridization again; After Seed Propagation in Southern Winter added generation, it was combined in ground such as Anyang, Shangqiu, Dezhou, Cangzhou and mixes choosing-miscegenation in 1992, selected 91 of individual plants in 1993; After pacing product and fiber quality test just, selected 16 of good plants in 1994; The nineteen ninety-five strain is comparative test, carries out the strain preliminary experiment in 1996, continues yielding ability, disease resistance, drought-enduring alkalescence and fiber quality are carried out the multianalysis test; 1997-1999 successively in Henan, the ecological tests in (autonomous region) saline land of provinceing or nonirrigated farmland such as Xinjiang, Shandong and Zhejiang, after the integration test analysis, select in the anti-salt new lines 8036.The advantage of this kind anti-saline and alkaline reaches under 0.4% condition at soil salt content, and the seedling rate 81.3% that becomes to live relatively reaches anti-salt level, has filled up the blank of the not anti-salt cotton variety of Henan Province and even China.
Embodiment 2 middle cotton 49 seed selection processes
Middle cotton 49 parental combinations be middle cotton institute 35 * in 51504.
Nineteen ninety-five be a female parent with middle cotton institute 35, in 51504 be that male parent makes up nineteen ninety-five F at this institute preparing hybrid
1For Sanya, seed Hainan in winter south numerous added-generation plantation 10 row, gather in the crops 4 kilograms of Westerners in March, 1996 altogether.
Its F in 1996
2In generation, respectively planted 0.2 mu on Earthquake of Anyang station in Henan, Shangqiu, Dezhou, Shandong, Cangzhou, Hebei, Kashi, Aksu and other places; And in Anyang sick garden has been planted 0.1 mu, each test site was mixed and was received 20-30 excellent strain late September, 1-2 bell only selected in every excellent strain; After all being recovered to Anyang; Mixing is carried out Seed Propagation in Southern Winter and is added generation plantation 20 row, and fiber send the test of cotton quality supervision and inspection center of the Ministry of Agriculture, selects altogether in March, 1996 and has received 6 kilograms of Westerners.
F in 1997
4The generation repetition is planted 0.3 mu in above-mentioned various places, continues to mix choosing, and combines the evaluation of disease garden, morning for technology such as fiber quality tests.Add generation plantation 30 row through Seed Propagation in Southern Winter, select altogether and received F in March, 1997
5Generation 8 kilograms of Westerners.
F in 1998
6In generation, is 2 mu of ground final elections of plantation in Anyang.Select 89 fine individual plants altogether, fiber send the test of cotton quality supervision and inspection center of the Ministry of Agriculture.
Carry out in Anyang plant-to-row test in 1999 and select 6 high yields, high-quality, disease-resistant strain system altogether.
Carried out that strain is comparative test in Anyang in 2000, in finally breeding 287.
Calendar year 2001 compares test in Earthquake of Anyang station in Henan and South Sinkiang alura.
2002-2003 participate in landlocked cotton region, national northwest early in ripe group of regional test, the gined cotton comparison is according to 35 raising the output 17.22% of middle cotton institute before the frost.Participated in the landlocked cotton variety production test in northwest in 2003, the gined cotton comparison is according to 35 raising the output 26.33% of kind middle cotton institute before the frost, and output all is positioned at the 1st of tested variety.The fibre length comparison has increased 1mm according to kind middle cotton institute 35, and specific strength has improved 2.2cN/tex, and the spinning evenness index has improved 8.3.The comprehensive proterties of this kind is excellent, has increased 2,700,000,000 yuan of wound economic results in society.
Embodiment 3 middle cotton 75 seed selection processes
Middle cotton institute 75 is a transgenic pest-resistant hybrid cotton kind.Parental combination be middle cotton institute 41 * in 9425.Middle cotton institute 41; Be that Cotton Inst., Chinese Agricultural Academy utilizes the Chinese Academy of Agricultural Sciences's biotechnology research to introduce the Bt+CpTI divalent insect-resistant gene that makes up; Import in the middle cotton institute 23 through pollen tube passage method; Form through seed selection for many years, in 2002 through the authorization of the national variety certification committee and obtain in Shandong, Henan, Anhui, Hebei etc. economize the agriculture genetically modified organism safety certificate of production application.In 9425 derive from middle cotton institute 35 * (in 324 * Soviet Union 8908), be high-quality, disease-resistant strain.
Nineteen ninety-five is in the preparing hybrid combination of this institute, and Seed Propagation in Southern Winter adds generation.
1996-1997 F
2Generation, F
4In generation, mixes on Earthquake of Anyang station in Henan, Shangqiu, Dezhou, Shandong, Cangzhou, Hebei, Kashi, Aksu and other places and selects miscegenation.F in 1998
6In generation,, 219 of the selected individual plants in field were through indoor species test and fiber quality test on 2 mu of ground of Earthquake of Anyang station in Henan plantation; Final final election goes out 83 individual plants, carries out plant-to-row test in 1999, and final election goes out 18 strain systems; 2000 carry out strain is comparative test, final election go out 1 major clique name in 9425.This strain shows as that bell is big, ginning outturn is higher, fabric is of fine quality, fibre length 32.6mm, specific strength 32.2cN/tex, mic value 4.2, high Hangzhoupro fusarium wilt, anti-verticillium wilt.
2001-2002 continuously to middle cotton institute 41 with in 9425 carry out the selfing purifying; Utilized in 2003 middle cotton institute 41 do maternal, in 9425 make male parent, the preparation combination also obtains among the first-filial generation kind sGKz 927; 2004-2005 respectively in Henan, Hebei, Shandong, 6 pilots in Anhui Province carries out crossbreed F
1For comparative test, carry out fiber quality test and disease-resistant, worm property evaluation the same period.2006-2007 participates in ripe Hybrid regional test (C group) in the cotton region, the Huanghe valley, ripe Hybrid production test (B group) in the participation cotton regions, the Huanghe valley in 2008.Obtained the agriculture genetically modified organism safety certificate of the production application in Henan Province in 2006, acquisition in 2008 is at the agriculture genetically modified organism safety certificate of cotton region, Huanghe valley production application.2009 through national crop varietal approval committee (the authorization numbering: state examines cotton 2009009).
Embodiment 4 middle cotton 79 seed selection processes
Middle cotton institute 79 (among the former code name sGK 177) parental combination be middle cotton institute 41 * in 4648.Wherein, middle cotton institute 41 is two valency transgenic pest-resistant cotton varieties, has the knot characteristics that bell property is strong, yielding ability is good; In 4648 be my institute's self-fertile the conventional cotton strain (51504 * Ji 668 in deriving from) of non-transgenic, have good, high anti-withered, the characteristics such as anti-Huang withers of fiber quality.In 51504 derive from (middle extra large 24 * 279) F
2* (Tamcot sp37 * brocade 444) F
2, wherein, middle extra large 24 is Shangqiu 24, and from the cotton 15 * sea-island cotton choosing of Mount Tai word system, 279 from the cotton No. 5 choosing systems of shen, and Tamcotsp37 is from texas,U.S high-quality, how anti-kind, and brocade 444 is from China northeast.
2000 respectively the cotton institute 41 of centering with in 4648 carried out the selfing purifying and improved.
Calendar year 2001 be female parent with middle cotton institute 41, in 4648 be male parent Anyang preparing hybrid make up middle cotton 41 * in 4648, and Seed Propagation in Southern Winter adds generation.2002-2003 F
2Generation, F
4In generation, mixes on Earthquake of Anyang station in Henan, Shangqiu, Kaifeng, Shandong Dongying, Shijiazhuang, Hebei, Hengshui, Kashi, Aksu and other places and selects miscegenation; And adopt the pest-resistant screening of 2000g/L kanamycin sulfate, morning for conventional breeding methods such as fiber quality test, disease resistance evaluation, F in 2004
6In generation,, 312 of the selected individual plants in field were through indoor species test and fiber quality test on 2 mu of ground of Earthquake of Anyang station in Henan plantation; Final final election goes out 112 individual plants; Carried out the plant comparative test in 2005,7 strains of final election were carried out variety comparative test in 2006; Bred among the tentative by name sGK of two valency transgenic cotton against pests new lines and to have obtained genetically modified organism safety certificate in December, 177,2006 in the production application in Henan Province.Carry out 4 times three in Earthquake of Anyang station in Henan, Zhoukou City, Zhengzhou, Kaifeng in 2007 and repeat to carry out the adaptability comparative test, carry out fiber test, disease resistance evaluation the same period.Participated in Henan Province's spring cotton variety (II group) regional test in 2008, continued to participate in the provinces and regions examination, and participate in production test simultaneously in 2009.Obtain genetically modified organism safety certificate in May, 2009 in the production application of cotton region, the Huanghe valley.Passed through Henan Province's crop varietal approval committee in 2010, naming is middle cotton institute 79.
This kind is the conventional spring cotton varieties of two valency transgenic pest-resistants.The 131 days time of infertility, the heavy 6.3g of single bell, seed refers to 10.9, ginning outturn 42.2%, flower rate 94.4% before the frost, the gined cotton comparison is extremely remarkable according to raising the output, is difficult for early ageing, anti-withered, anti-verticillium wilt, fibre length 30.6mm, fracture specific strength 29.0cN/tex, mic value 4.9.Suitable Henan Province and cotton region, the Huanghe valley spring is live or the wheat-cotton intercropping plantation.
Claims (1)
1. many ecotope wheel back-mixing choosing-miscegenation cotton breeding methods is characterized in that, said method comprising the steps of:
1) hybrid seeding is carried out in preparation cotton male parent, hybridization of female parent combination;
2) F
1Add generation for the crossbreed Seed Propagation in Southern Winter;
3) F
2For seed divide kind in the Huanghe valley, the landlocked cotton region in the Yangtze river basin and northwest;
4) choose F
2The fine individual plant in generation mixes receives, i.e. F
3For seed, and south numerous added-generation;
5) F
4For seed divide kind in the Huanghe valley again, the landlocked cotton region in the Yangtze river basin and northwest;
6) choose F
4The fine individual plant in generation mixes receives, i.e. F
5For seed, and south numerous added-generation;
7) choose F
5The fine individual plant in generation mixes receives, i.e. F
6For seed; The plantation on hybrid seeding ground, and according to breeding objective selection fine individual plant, selected individual plant further carries out plant, strain system and variety comparative test; The offspring of selfing in continuous 2 years; Detect purity through comparative test and classification marking, breed through isolation simultaneously and guarantee purity, thereby seed selection obtains new cotton variety.
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| CN104620979A (en) * | 2015-02-12 | 2015-05-20 | 重庆市中药研究院 | Breeding method of combining sweet wormwood group breeding with multi-ecological hybrid breeding |
| CN105230179A (en) * | 2015-09-28 | 2016-01-13 | 河北省农林科学院旱作农业研究所 | Multi-stress synchronous stress breeding method for cotton breeding |
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| CN116058280A (en) * | 2023-02-03 | 2023-05-05 | 中国农业科学院棉花研究所 | Synergistic breeding method of excellent parent and new variety of widely-adapted cotton |
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| CN104082125B (en) * | 2014-07-01 | 2015-12-30 | 中国农业科学院棉花研究所 | A kind of breeding method improving output of cotton and quality |
| CN104620979A (en) * | 2015-02-12 | 2015-05-20 | 重庆市中药研究院 | Breeding method of combining sweet wormwood group breeding with multi-ecological hybrid breeding |
| CN105230179A (en) * | 2015-09-28 | 2016-01-13 | 河北省农林科学院旱作农业研究所 | Multi-stress synchronous stress breeding method for cotton breeding |
| CN105230179B (en) * | 2015-09-28 | 2019-07-02 | 河北省农林科学院旱作农业研究所 | A kind of more adverse circumstances of breeding cotton are synchronous to coerce method of breeding |
| CN105284591A (en) * | 2015-09-30 | 2016-02-03 | 武汉隆福康农业发展有限公司 | Breeding method for limited fruit branch type short-season cotton suitable for being planted in Yangtze River valley cotton areas |
| CN105409762A (en) * | 2015-12-31 | 2016-03-23 | 中国农业科学院棉花研究所 | Cyclic breeding method of cotton varieties |
| CN105409762B (en) * | 2015-12-31 | 2018-01-30 | 中国农业科学院棉花研究所 | A kind of samsara selection of cotton variety |
| WO2022083511A1 (en) * | 2020-10-21 | 2022-04-28 | 湖南省棉花科学研究所 | Breeding method for new transgenic high-quality species of upland cotton |
| US11910768B2 (en) | 2020-10-21 | 2024-02-27 | Cotton Science Research Institute Of Hunan | Method for breeding a new variety of high-quality Gossypium hirsutum |
| CN113498734A (en) * | 2021-06-29 | 2021-10-15 | 辽宁省经济作物研究所 | Non-transgenic cotton breeding method |
| CN116058280A (en) * | 2023-02-03 | 2023-05-05 | 中国农业科学院棉花研究所 | Synergistic breeding method of excellent parent and new variety of widely-adapted cotton |
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