CN102365951A - Application of chitosan oligosaccharide for promoting growth of crop rhizomes - Google Patents
Application of chitosan oligosaccharide for promoting growth of crop rhizomes Download PDFInfo
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- CN102365951A CN102365951A CN 201110333658 CN201110333658A CN102365951A CN 102365951 A CN102365951 A CN 102365951A CN 201110333658 CN201110333658 CN 201110333658 CN 201110333658 A CN201110333658 A CN 201110333658A CN 102365951 A CN102365951 A CN 102365951A
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- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 title claims abstract description 89
- 230000012010 growth Effects 0.000 title claims abstract description 43
- 230000001737 promoting effect Effects 0.000 title claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 33
- 230000008635 plant growth Effects 0.000 claims description 23
- 241000196324 Embryophyta Species 0.000 claims description 13
- 238000002791 soaking Methods 0.000 claims description 9
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims description 7
- 240000008067 Cucumis sativus Species 0.000 claims description 5
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 240000007087 Apium graveolens Species 0.000 claims description 3
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 claims description 3
- 235000010591 Appio Nutrition 0.000 claims description 3
- 235000007319 Avena orientalis Nutrition 0.000 claims description 3
- 235000007558 Avena sp Nutrition 0.000 claims description 3
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 3
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims description 3
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims description 3
- 244000241257 Cucumis melo Species 0.000 claims description 3
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 244000017020 Ipomoea batatas Species 0.000 claims description 3
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 244000046109 Sorghum vulgare var. nervosum Species 0.000 claims description 3
- 244000300264 Spinacia oleracea Species 0.000 claims description 3
- 235000009337 Spinacia oleracea Nutrition 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 244000098338 Triticum aestivum Species 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 241000209763 Avena sativa Species 0.000 claims 1
- 244000241235 Citrullus lanatus Species 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 241000219109 Citrullus Species 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000003337 fertilizer Substances 0.000 description 5
- 229920000642 polymer Polymers 0.000 description 4
- 230000002786 root growth Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 3
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 229960002442 glucosamine Drugs 0.000 description 3
- 150000002482 oligosaccharides Polymers 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 244000075850 Avena orientalis Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 238000002144 chemical decomposition reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 125000003147 glycosyl group Chemical group 0.000 description 2
- 230000003054 hormonal effect Effects 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000006196 deacetylation Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to an application of chitosan oligosaccharide for promoting growth of crop rhizomes, in particular to an application of chitosan oligosaccharide for promoting growth of aboveground plants and growth of root at the growing initial stage of crops, wherein the chitosan oligosaccharide can be dry chitosan oligosaccharide powder, a chitosan oligosaccharide solution or other preparations of chitosan oligosaccharide; and the concentration of the chitosan oligosaccharide solution is 5-25 ppm. The novel application of the chitosan oligosaccharide provides a new direction and method for growth and regulation of the crops, and is beneficial to regulation of the growth of crops by applying a special chemical reagent.
Description
Technical field
The invention belongs to agrochemical field, especially relate to of the application of a kind of chitosan oligosaccharide, be specifically related to the purposes that chitosan oligosaccharide is used to promote the crop root growth in plant growth adjusting field.
Background technology
Found much can influence the material of plant growth at present, like hormonal substance, it can be in the growth that influences crop aspect certain; Some material also; Like nutriment, necessary material base is provided for the growth of crop, to replenish the limitation of nutritional deficiency in soil or the air.
For the research of chitosan oligosaccharide, find just that generally it can promote plant growth, but for the qualitative promotion Study on Growth of chitosan oligosaccharide seldom.
The present invention is surprised to find that chitosan oligosaccharide has produced a kind of unforeseeable influence mode to the growth of crop, and this influence mode can be realized the effective adjusting to plant growth.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of new purposes of chitosan oligosaccharide, concrete is to the invention provides the purposes that a kind of chitosan oligosaccharide is used for promoting at the plant growth initial stage ground plant strain growth and the growth of root length.
Chitosan oligosaccharide provided by the invention is used for promoting the purposes that plant strain growth and root length are on the ground grown at the plant growth initial stage, and wherein the plant growth initial stage is meant that crop seed sprouts to the time in preceding 1/15th to 1/5th vegetative period of crop.
Wherein chitosan oligosaccharide can be a chitosan oligosaccharide dry powder, chitosan oligosaccharide solution, and other preparation of the for example chitosan oligosaccharide aqueous solution, or chitosan oligosaccharide is like chitosan oligosaccharide aqueous suspension agent, aqueous emulsion etc.If adopt chitosan oligosaccharide solution, then the concentration of chitosan oligosaccharide solution is less than 25ppm.Preferably, the concentration of chitosan oligosaccharide solution is 5-25ppm.
Concretely, the general plant growth initial stage is meant from the crop seed sprouting and begins to calculate, and by plant growth 5-20 days, for example, in the average out to 80-85 days vegetative period of watermelon, the early growth period of watermelon was meant that the watermelon seed sprouting grows into 6 days to 17 days to it so.So calculate the plant growth initial stage.
In the such use, crop is the conventional crop of agriculture field, for example, cereal crops, like corn, paddy rice, wheat, oat, Chinese sorghum, soybean, potato, Ipomoea batatas etc., vegetable crop, like spinach, celery, Chinese cabbage, rape, cucumber etc., other crop such as watermelon, muskmelon.Be preferably corn and watermelon.
Above-mentioned chitosan oligosaccharide is used to promote ground plant strain growth and the growth of root length to be not limited to concrete method of application and administration form at the plant growth initial stage.Can be to soak seed, perhaps chitosan oligosaccharide is applied to the crop seedling of early growth period.
The present invention also provides a kind of chitosan oligosaccharide solution promotes ground plant strain growth and the growth of root length at the plant growth initial stage method of using; It is characterized in that using chitosan oligosaccharide solution soaking seed; Perhaps chitosan oligosaccharide solution is applied to the crop seedling of early growth period, wherein the concentration of chitosan oligosaccharide solution is 5-25ppm.
Preferably; Chitosan oligosaccharide promotes plant strain growth on the ground at the plant growth initial stage, and the growth of root length can realize with the chitosan oligosaccharide solution soaking through the seed to crop, just before crop breeding; Seed is soaked in the chitosan oligosaccharide solution; The concentration of chitosan oligosaccharide solution is not restriction especially generally, and according to crop sequences, the concentration of chitosan oligosaccharide solution can be adjusted.With the also not restriction especially of time of chitosan oligosaccharide solution soaking seed, also be according to crop sequences, the time that adjustment is soaked.
The present invention finds; For example, perhaps chitosan oligosaccharide solution is used crop seedling, realized influence the growth of crop initial stage through chitosan oligosaccharide solution soaking seed; And this influence is not the influence of single direction; Neither randomly influence, its ground plant strain growth and root growth that influences crop is obvious unusually, just can promote the ground plant strain growth and the growth of root length.The energy that this influence is actually the adjustment seed distributes to the different directions of growth, at the plant growth initial stage, hopes that plant grows fast on the seed-breeding field, and then the photosynthesis of enhancing crop, improves the immunity of crop.
As far as general crop, the early growth period ambient temperature of crop is lower, and the quick growth of plant on the ground can be strengthened photosynthesis; This work is realized in order to promote root length to grow; So chitosan oligosaccharide is regulated the material of this growth just, this discovery has all of a sudden obtained to realize the active principle of this influence, is exactly chitosan oligosaccharide; And for chitosan oligosaccharide, also be to find that it can influence the new purposes of plant growth mode.
Among the present invention, chitosan oligosaccharide is that Glucosamine passes through 1, and the degree of polymerization that the 4-glycosidic bond is formed by connecting is the mixture of the high molecular polymer of 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be through any means preparation, and for example following method: deacetylation is the mixture of the chitosan oligomer of 2-30 at the shitosan more than 70% through the degree of polymerization that methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Perhaps, be the mixture of the chitosan oligomer of 2-30 through from fungal cell wall or insect body wall, extracting the natural chitin obtain and the degree of polymerization for preparing through methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods.Perhaps, through passing through 1 by Glucosamine, the degree of polymerization that the chemosynthesis of 4-glycosidic bond is polymerized is the mixture of polymers of 2-30.Chitosan oligosaccharide is the mixture of the high molecular polymer of various polymerization thing at present, that is to say in the chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization possibly exist all.So no matter prepare through which kind of method; The commercially available prod that perhaps prepares through above-mentioned preparation method, as long as it is that Glucosamine passes through 1, the degree of polymerization that the 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly a chitosan oligosaccharide of the present invention; It can contain acetyl group; But the sugar unit of bonding acetyl group is no more than 40% amount, preferably is no more than 30% amount, especially preferably is no more than 20% amount.Commercially available chitosan oligosaccharide all is the chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth basically at present.
The preparation of chitosan oligosaccharide is a lot of after deliberation, and achievement in research of the present invention is found, no matter is that the chitosan oligosaccharide which kind of method is produced is applied among the present invention, and it can both produce described effect.
Among the present invention; Chitosan oligosaccharide solution generally is meant the aqueous solution of chitosan oligosaccharide; Or other solution of chitosan oligosaccharide, the solvent in this solution can dissolve chitosan oligosaccharide, and guarantees that chitosan oligosaccharide is when being applied to crop; Solvent can be absorbed, utilizes, infiltrate soil as nutriment by crop, perhaps evaporate in the air.
For realizing that the plant growth initial stage is promoted the crop aerial growth and promotes the growth of root length; Can use chitosan oligosaccharide solution separately; The nutriment that also can in chitosan oligosaccharide solution, add other; Like fertilizer, inorganic fertilizer or inorganic composite fertilizer, also can use fertilizer and inorganic fertilizer simultaneously.The hormonal substance that also can in chitosan oligosaccharide solution, add other desire control crop particular growth is like growth hormone, gibberellin, ethene etc.Be needs, as prevent adverse effects such as the sick or insect infestation of crop, can in chitosan oligosaccharide solution, add bactericide or insecticide.Because the physical chemistry of chitosan oligosaccharide is specific; Other material that adds does not receive the influence of chitosan oligosaccharide aspect physico chemistry; Just chitosan oligosaccharide can not reduce the activity of other material; On the contrary maybe be because chitosan oligosaccharide when influencing plant growth, promote the influence of other material to crop, this influence generally is positive influence.
The new purposes of chitosan oligosaccharide of the present invention is regulated a new direction and the method for providing for plant growth, and the specific chemical reagent that helps using is regulated plant growth.
Embodiment
In order to understand the present invention, further specify the present invention with embodiment below, but do not limit the present invention.
Embodiment 1:
5% chitosan oligosaccharide solution with water is diluted to application corresponding concentration respectively; Concentration is calculated according to weight percent concentration; In outcome record, calculate with ppm (1,000,000/) concentration, what 50ppm represented in the outcome record below is that weight percent concentration is 0.005%.
The cucumber seeds of choosing full seed soaks 24h respectively with the chitosan oligosaccharide and the distilled water seed soaking of different extension rates in 25 ℃ of insulating boxs.Each handles 30,3 repetitions.Use distilled water flushing then 3 times, place the culture dish that is covered with 3 metafiltration paper, add 10ml distilled water, in 25 ℃ of insulating boxs, sprouted 10 days under the dark surrounds, the long and plant height of statistical survey root.
Table 1 variable concentrations oligosaccharide seed soaking is to the influence of corn root growth
Can find out that from table 1 chitosan oligosaccharide all has facilitation in the variable concentrations seed soaking that is lower than 25ppm to the aerial growth of the early growth period of cucumber and the length of following of under ground portion, promotes the growth of the cauline leaf of plant on the ground and the growth of underground root.
Embodiment 2: other crop uses chitosan oligosaccharide to promote the experiment of aerial growth and the elongation of underground root
According to the experimental technique of embodiment 1, to paddy rice, wheat, oat, Chinese sorghum; Soybean, potato, Ipomoea batatas, spinach, celery; Chinese cabbage, rape, cucumber, watermelon and muskmelon are done experiment respectively, these crop applying chitosan oligosaccharides; Comprise the seed of crop soaked seed with chitosan oligosaccharide solution, and that at seedling spraying chitosan oligosaccharide solution the concentration of all finding chitosan oligosaccharide solution is lower than 25ppm when handling, and the acrial part plant strain growth of crop and the elongation of under ground portion root are all had facilitation.
Claims (8)
1. a chitosan oligosaccharide is used for promoting the purposes that ground plant strain growth and root length are grown at the plant growth initial stage.
2. purposes according to claim 1, wherein the plant growth initial stage is meant that crop seed sprouts to the time in preceding 1/15th to 1/5th vegetative period of crop.
3. purposes according to claim 1, wherein chitosan oligosaccharide is chitosan oligosaccharide dry powder or chitosan oligosaccharide solution.
4. purposes according to claim 3, wherein chitosan oligosaccharide solution is the chitosan oligosaccharide aqueous solution.
5. according to claim 3 or 4 described purposes, wherein the concentration of chitosan oligosaccharide solution is 5-25ppm.
6. according to each described purposes of claim 1-5, wherein crop is a corn, paddy rice, wheat, oat, Chinese sorghum, soybean, potato, Ipomoea batatas, spinach, celery, Chinese cabbage, rape, cucumber, watermelon, muskmelon.
7. according to each described purposes of claim 1-5, wherein the method for application of chitosan oligosaccharide and administration form perhaps are applied to chitosan oligosaccharide the crop seedling of early growth period for soaking seed.
8. use chitosan oligosaccharide solution promotes ground plant strain growth and the growth of root length at the plant growth initial stage method for one kind; It is characterized in that using chitosan oligosaccharide solution soaking seed; Perhaps chitosan oligosaccharide solution is applied to the crop seedling of early growth period, wherein the concentration of chitosan oligosaccharide solution is 5-25ppm.
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| CN 201110333658 CN102365951B (en) | 2011-10-28 | 2011-10-28 | Application of chitosan oligosaccharide for promoting growth of crop rhizomes |
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| CN 201110333658 CN102365951B (en) | 2011-10-28 | 2011-10-28 | Application of chitosan oligosaccharide for promoting growth of crop rhizomes |
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| CN102365951B CN102365951B (en) | 2013-03-06 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430944A (en) * | 2013-09-15 | 2013-12-11 | 海南正业中农高科股份有限公司 | Application of chitosan oligosaccharide-containing composition to promotion of swelling of subterraneous stems and tuberous roots of plants |
| CN105076135A (en) * | 2015-09-22 | 2015-11-25 | 中国科学院海洋研究所 | Chitosan oligosaccharide regulator for promoting photosynthesis and growth of wheat |
| CN114766479A (en) * | 2022-06-20 | 2022-07-22 | 山东多芬农业有限公司 | Preparation method of biological stimulator for promoting plant growth and development |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1470168A (en) * | 2003-06-23 | 2004-01-28 | 华东理工大学 | Chioligose plant grow-promotor and its preparation method |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1470168A (en) * | 2003-06-23 | 2004-01-28 | 华东理工大学 | Chioligose plant grow-promotor and its preparation method |
Non-Patent Citations (3)
| Title |
|---|
| 《中国农学通报》 20090205 郭卫华等 壳寡糖对黄瓜种子萌发和幼苗生长及光合特性的影响 165-166 1-4,6-8 , 第03期 * |
| 《安徽农业科学》 20110110 袁建平等 壳寡糖对玉米种子萌发及幼苗生长的影响 88 1-8 , 第01期 * |
| 《安徽农业科学》 20110120 袁建平等 壳寡糖对小麦种子萌发及幼苗生长的影响 709-710 1-8 , 第02期 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430944A (en) * | 2013-09-15 | 2013-12-11 | 海南正业中农高科股份有限公司 | Application of chitosan oligosaccharide-containing composition to promotion of swelling of subterraneous stems and tuberous roots of plants |
| CN105076135A (en) * | 2015-09-22 | 2015-11-25 | 中国科学院海洋研究所 | Chitosan oligosaccharide regulator for promoting photosynthesis and growth of wheat |
| CN114766479A (en) * | 2022-06-20 | 2022-07-22 | 山东多芬农业有限公司 | Preparation method of biological stimulator for promoting plant growth and development |
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| CN102365951B (en) | 2013-03-06 |
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Address after: 571924 the development of Hainan County of Chengmai province old town village distribution Patentee after: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Address before: Four, building 25, glorious cinema tower, 570206 Nansha Road, Longhua District, Hainan, Haikou Patentee before: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. |
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Denomination of invention: Application of chitosan oligosaccharide for promoting growth of crop rhizomes Effective date of registration: 20151214 Granted publication date: 20130306 Pledgee: Qiongshan branch of Agricultural Development Bank of Haikou city China Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2015460000005 |
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