CN102358881A - Disposable anaerobe culturing flat plate - Google Patents
Disposable anaerobe culturing flat plate Download PDFInfo
- Publication number
- CN102358881A CN102358881A CN2011103309738A CN201110330973A CN102358881A CN 102358881 A CN102358881 A CN 102358881A CN 2011103309738 A CN2011103309738 A CN 2011103309738A CN 201110330973 A CN201110330973 A CN 201110330973A CN 102358881 A CN102358881 A CN 102358881A
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- ware
- bag
- disposable
- dull
- pallet
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
- C12M29/22—Oxygen discharge
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Abstract
The invention discloses a disposable anaerobe culturing flat plate, comprising a vessel body (1), a vessel cover (2) and a tray (3), wherein an opening is formed on an upper end of the vessel body (1), the vessel cover (2) is connected with the vessel body (1) and is used for sealing the opening of the vessel body (1), and the vessel body (1) is internally provided with an anaerobe culturing medium layer (4). The tray (3) is provided with an open hole penetrating the upper end face and the lower end face of the tray (3), a sealing bag (5) is arranged on the upper end of the tray (3) and is internally provided with a deoxidizing venting bag (6) internally containing oxygen uptaking substances as well as a carbon dioxide generating bag (7) internally containing chemical substances which react with water to generate carbon dioxide. By adopting such structure in the invention, the disposable anaerobe culturing flat plate has a simple integral structure and low cost, is convenient to operate, and can be used for generating an anaerobic environment rapidly and effectively reducing the time for exposing anaerobes inside an aerobic environment, and thus, the disposable anaerobe culturing flat plate can be configured, sold and used on a scale and is low in configuration and storage cost.
Description
Technical field
The present invention relates to the culture apparatus of anerobes, specifically is that disposable anaerobic bacteria culture is dull and stereotyped.
Background technology
Anaerobism incubator and anaerobism culture bag are mainly adopted in the present stage cultivation of anerobes.The anaerobism incubator is that the mode through substitution gas makes its inside reach anaerobic environment, needs to be equipped with displacement in addition with rare gas element and dioxide gas, and equipment drops into height, and complicated operation is too high for a spot of anaerobic bacteria culture cost.The anaerobism bag is through deoxygenation reagent bag air in the sealing bag to be carried out deoxygenation; Thereby make and reach anaerobic environment in the sealing bag; But because the anaerobism bag directly is not positioned in the petridish; Therefore slow to the oxygen removal process in the plate, need the long time, the time has been grown then can be extremely unfavorable to the growth of anerobes.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, provide a kind of simple in structure, cost is low, be convenient to operation, can produce anaerobic environment fast, thereby effectively reduce the disposable anaerobism culture plate that anerobes is exposed to the time in the aerobic environment.
The object of the invention is mainly realized through following technical scheme: disposable anaerobic bacteria culture is dull and stereotyped; Comprise ware body, ware lid and the pallet that is connected with the ware lid; The upper end of said ware body is provided with opening; Said ware lid and ware body are connected and seal the opening of ware body, are provided with the anaerobic culture medium layer in the said ware body, and it is interior and between anaerobic culture medium layer and ware cover that said pallet is arranged on the ware body; Said pallet is provided with the perforate that runs through its upper and lower end face, and the upper end of pallet is provided with sealing bag, establishes the deoxygenation venting bags of oxygen-absorbing substance in being provided with in the said sealing bag, and in establish the carbonic acid gas that generates the chemical substance of carbonic acid gas with water reaction and generate bag.The oxygen-absorbing substance that is provided with in the deoxygenation venting bags is preferably active metal particle and carbonaceous molecular sieve; Wherein, Oxidizing reaction can take place when the active metal particle contacts with oxygen in the preferred iron powder of active metal particle, zinc powder or the mixture of the two, consumes oxygen in the ware body; Simultaneously carbonaceous molecular sieve also can within a short period of time, the intravital oxygen of absorption ware.Carbonic acid gas generates the chemical substance that is provided with in the bag and is preferably sour hydrogen sodium and Hydrocerol A powder, when the planar water steam, and water vapour in sodium hydrogencarbonate and the Hydrocerol A powder absorption ware body, and reaction generates carbonic acid gas.
Said ware covers and is connected with buckle, is equipped with projection on said ware body and the buckle, and the projection upper surface of said buckle contacts with the projection lower surface of ware body.
The inner top of said ware lid constitutes supporting station, and the sidewall upper of said pallet constitutes the platform that articulates of evagination, and said pallet is through being connected with the ware lid on the supporting station that articulates platform and be arranged on the ware lid.
Be provided with two ventilative paper bags in the said sealing bag, said deoxygenation venting bags and carbonic acid gas generate bag and are separately positioned in two ventilative paper bags.
The quantity that said deoxygenation venting bags and carbonic acid gas generate bag is more than one.
Said ventilative paper bag, deoxygenation venting bags and carbonic acid gas generate bag and process by non-woven fabrics filter paper.
Said sealing bag is an aluminium plastic packaging bag.
Be provided with sealring between the connecting portion of said ware lid and ware body.
The present invention compared with prior art has the following advantages: (1) the present invention includes ware body, ware lid, pallet and sealing bag, and the ware lid is tightly connected with the ware body, is provided with the anaerobic culture medium layer in the ware body; Be provided with deoxygenation venting bags and carbonic acid gas in the sealing bag and generate bag, the present invention adopts said structure, and one-piece construction is simple; Cost is low, is convenient to deposit anaerobic culture medium, when need are cultivated anerobes; Can cover the ware lid behind the opening encapsulation lid again through opening the ware lid, oxygen-absorbing substance absorbs oxygen in the deoxygenation venting bags; Carbonic acid gas generates chemical substance and water reaction generation dioxide gas in the bag, can generate anaerobic environment fast thereby make in the ware body, has effectively reduced anerobes because of being exposed to for a long time in the aerobic environment; Thereby the anerobes that avoids anaerobic culture medium to generate is dead, has improved the recall rate of anerobes; The present invention can carry out commercialization, mass-producing configuration, and retain costs is low.
(2) pallet of the present invention is through being connected with the ware lid on the supporting station that articulates platform and be arranged on ware lid, and pallet is connected with the ware lid or covers disengaging conveniently from ware, is convenient to picking and placeing of pallet.
Description of drawings
Fig. 1 is the sectional structure synoptic diagram of the embodiment of the invention.
The pairing name of Reference numeral is called in the accompanying drawing: 1, ware body, 2, the ware lid, 3, pallet, 4, the anaerobic culture medium layer; 5, sealing bag, 6, the deoxygenation venting bags, 7, carbonic acid gas generates bag, 8, buckle; 9, sealring, 10, supporting station, 11, articulate platform, 12, ventilative paper bag.
Embodiment
Below in conjunction with embodiment and accompanying drawing the present invention is done further detailed description, but embodiment of the present invention is not limited thereto.
Embodiment:
As shown in Figure 1; Disposable anaerobic bacteria culture is dull and stereotyped; Comprise ware body 1 and the ware lid 2 that is tightly connected with ware body 1, the upper end of ware body 1 is provided with opening, and ware lid 2 and ware body 1 are connected and seal the opening of ware body 1; In order to guarantee the sealing property of ware body 1 and ware lid between 2, be provided with sealring 9 between both connecting portions of ware lid 2 and ware body 1.For fastening ware body 1 covers being connected between 2 with ware; Be connected with buckle 8 on the ware lid 2; Be equipped with projection on ware body 1 and the buckle 8; The projection upper surface of buckle 8 contacts with the projection lower surface of ware body 1, and the projection of ware body 1 is around the outer side wall setting of ware body 1, and buckle 8 also is provided with the projection corresponding with the bump position of ware body 1.Ware lid 2 is connected with pallet 3; The inner top of ware lid 2 constitutes supporting station 10; What the sidewall upper of pallet 3 constituted evagination articulates platform 11; Pallet 3 is through being connected with ware lid 2 on the supporting station 10 that articulates platform 11 and be arranged on ware lid 2, and the central part of pallet 3 is provided with the perforate that runs through its upper and lower end face, is convenient to gas communication.Be provided with anaerobic culture medium layer 4 in the ware body 1; The anaerobic culture medium of anaerobic culture medium layer 4 selects to be fit to the nutrient environment that anerobes grows fast, and select the nutrient media components of cultivation to be as being fit to produce gas pod membrane acid bacterium: proteolysate, yeast soak powder, S-WAT, polymyxin, Sulphadiazine Sodium, ironic citrate, pancreas Suning and agar powder.When pallet 3 was arranged in the ware body 1, it was between anaerobic culture medium layer 4 and ware lid 2.
The upper end of pallet 3 is provided with sealing bag 5; The sealing bag 5 preferred aluminium plastic packaging bags that adopt; Sealing bag 5 is between pallet 3 and ware lid 2; Be provided with in the sealing bag 5 in 12, one ventilative paper bags 12 of two ventilative paper bags and be provided with more than one deoxygenation venting bags 6, be provided with more than one carbonic acid gas in another ventilative paper bag 12 and generate bag 7.Ventilative paper bag 12, deoxygenation venting bags 6 and carbonic acid gas generate bag 7 and process by non-woven fabrics filter paper.Be provided with oxygen-absorbing substance in the deoxygenation venting bags 6; The oxygen-absorbing substance that is provided with in the deoxygenation venting bags 6 is preferably active metal particle and carbonaceous molecular sieve, wherein, and the preferred iron powder of active metal particle, zinc powder or the mixture of the two; Oxidizing reaction can take place when the active metal particle contacted with oxygen; Consume oxygen in the ware body, carbonaceous molecular sieve also can within a short period of time simultaneously, the intravital oxygen of absorption ware.Carbonic acid gas generates and to be provided with the chemical substance that generates carbonic acid gas with the water reaction in the bag 7; Carbonic acid gas generates the material that is provided with in the bag 7 and is preferably sour hydrogen sodium and Hydrocerol A powder; When the planar water steam; Water vapour in sodium hydrogencarbonate and the Hydrocerol A powder absorption ware body, and reaction generates carbonic acid gas.Wherein, iron powder and oxygen reaction generate red oxide of iron, and zinc powder and oxygen reaction generate zinc oxide, and sodium hydrogencarbonate and Hydrocerol A powder and water reaction generate Trisodium Citrate, water and carbonic acid gas.
The principle of work that disposable anaerobic bacteria culture is dull and stereotyped: the anaerobic culture medium that configures is placed in the ware body 1, when to be used, the projection of buckle 8 and the projection of ware body 1 is broken away from; After taking off ware lid 2 pallet 3 is taken off from ware lid 2; Open behind the sealing bag 5 and again pallet 3 is articulated on the ware lid 2, again ware lid 2 is connected with ware body 1, being connected between fastening buckle 8 and the ware body 1; Oxygen-absorbing substance in the deoxygenation venting bags 6 absorbs oxygen; Carbonic acid gas generates chemical substance and water reaction generation carbonic acid gas in the bag 7, and oxygen-free environment generates rapidly in the ware body 1, and the anaerobic culture medium of anaerobic culture medium layer 4 generates anerobes.
As stated, then can well realize the present invention.
Claims (8)
1. disposable anaerobic bacteria culture is dull and stereotyped; It is characterized in that: comprise ware body (1), ware lid (2) and the pallet (3) that is connected with ware lid (2); The upper end of said ware body (1) is provided with opening; Said ware lid (2) and ware body (1) are connected and seal the opening of ware body (1), are provided with anaerobic culture medium layer (4) in the said ware body (1), and it is interior and be positioned at anaerobic culture medium layer (4) and ware covers between (2) that said pallet (3) is arranged on ware body (1); Said pallet (3) is provided with the perforate that runs through its upper and lower end face; The upper end of pallet (3) is provided with sealing bag (5); Establish the deoxygenation venting bags (6) of oxygen-absorbing substance in being provided with in the said sealing bag (5), and in establish the carbonic acid gas that generates the chemical substance of carbonic acid gas with water reaction and generate bag (7).
2. disposable anaerobic bacteria culture according to claim 1 is dull and stereotyped; It is characterized in that: be connected with buckle (8) on the said ware lid (2); Be equipped with projection on said ware body (1) and the buckle (8), the projection upper surface of said buckle (8) contacts with the projection lower surface of ware body (1).
3. disposable anaerobic bacteria culture according to claim 1 is dull and stereotyped; It is characterized in that: the inner top of said ware lid (2) constitutes supporting station (10); What the sidewall upper of said pallet (3) constituted evagination articulates platform (11), and said pallet (3) is gone up through the supporting station (10) that articulates platform (11) and be arranged on ware lid (2) and covered (2) with ware and be connected.
4. disposable anaerobic bacteria culture according to claim 1 is dull and stereotyped; It is characterized in that: be provided with two ventilative paper bags (12) in the said sealing bag (5), said deoxygenation venting bags (6) and carbonic acid gas generate bag (7) and are separately positioned in two ventilative paper bags (12).
5. disposable anaerobic bacteria culture according to claim 4 is dull and stereotyped, it is characterized in that: the quantity that said deoxygenation venting bags (6) and carbonic acid gas generate bag (7) is more than one.
6. disposable anaerobic bacteria culture according to claim 4 is dull and stereotyped, it is characterized in that: said ventilative paper bag (12), deoxygenation venting bags (6) and carbonic acid gas generate bag (7) and process by non-woven fabrics filter paper.
7. dull and stereotyped according to described any the disposable anaerobic bacteria culture of claim 1～6, it is characterized in that: said sealing bag (5) is an aluminium plastic packaging bag.
8. dull and stereotyped according to described any the disposable anaerobic bacteria culture of claim 1～6, it is characterized in that: be provided with sealring (9) between said ware lid (2) and both connecting portions of ware body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110330973 CN102358881B (en) | 2011-10-27 | 2011-10-27 | Disposable anaerobe culturing flat plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110330973 CN102358881B (en) | 2011-10-27 | 2011-10-27 | Disposable anaerobe culturing flat plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102358881A true CN102358881A (en) | 2012-02-22 |
| CN102358881B CN102358881B (en) | 2013-01-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110330973 Expired - Fee Related CN102358881B (en) | 2011-10-27 | 2011-10-27 | Disposable anaerobe culturing flat plate |
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| Country | Link |
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| CN (1) | CN102358881B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694445A (en) * | 2015-04-01 | 2015-06-10 | 黑龙江大学 | Composite deoxidation layer of anaerobic micro-organism culture medium |
| CN106754248A (en) * | 2016-12-27 | 2017-05-31 | 重庆迪赛生物工程有限公司 | A kind of anaerobic culture device |
| CN107794223A (en) * | 2017-11-07 | 2018-03-13 | 广东药科大学附属第医院 | Anaerobic bacteria and the in vitro study model and method of aerobic cell interaction in cell co-culture device and analogue body |
| CN109825425A (en) * | 2019-02-19 | 2019-05-31 | 江苏中新医药有限公司 | A kind of air-tightness culture dish of generation and maintenance anaerobic or micro- oxygen microenvironment |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1026253A (en) * | 1964-11-27 | 1966-04-14 | St Luke S Hospital Res Foundat | Anaerobic culturing device |
| FR2563232A1 (en) * | 1984-04-18 | 1985-10-25 | Claeys Christian | Dish cover for microbiological culture under a controlled atmosphere. |
| US6204051B1 (en) * | 1994-05-04 | 2001-03-20 | Oxyrase, Inc. | Apparatus and method for growing anaerobic microorganisms |
| CN2652938Y (en) * | 2003-09-10 | 2004-11-03 | 芦森 | Anaerobe cultivation medium |
| CN2732750Y (en) * | 2004-10-27 | 2005-10-12 | 贝瑞特生物技术（郑州）有限责任公司 | Culture utensil for anaerobic culture |
| CN201530826U (en) * | 2009-10-28 | 2010-07-21 | 陈文永 | Culture dish |
| CN202246684U (en) * | 2011-10-27 | 2012-05-30 | 成都瑞琦科技实业有限责任公司 | Disposable anaerobic bacteria culture plate |
-
2011
- 2011-10-27 CN CN 201110330973 patent/CN102358881B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1026253A (en) * | 1964-11-27 | 1966-04-14 | St Luke S Hospital Res Foundat | Anaerobic culturing device |
| FR2563232A1 (en) * | 1984-04-18 | 1985-10-25 | Claeys Christian | Dish cover for microbiological culture under a controlled atmosphere. |
| US6204051B1 (en) * | 1994-05-04 | 2001-03-20 | Oxyrase, Inc. | Apparatus and method for growing anaerobic microorganisms |
| CN2652938Y (en) * | 2003-09-10 | 2004-11-03 | 芦森 | Anaerobe cultivation medium |
| CN2732750Y (en) * | 2004-10-27 | 2005-10-12 | 贝瑞特生物技术（郑州）有限责任公司 | Culture utensil for anaerobic culture |
| CN201530826U (en) * | 2009-10-28 | 2010-07-21 | 陈文永 | Culture dish |
| CN202246684U (en) * | 2011-10-27 | 2012-05-30 | 成都瑞琦科技实业有限责任公司 | Disposable anaerobic bacteria culture plate |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694445A (en) * | 2015-04-01 | 2015-06-10 | 黑龙江大学 | Composite deoxidation layer of anaerobic micro-organism culture medium |
| CN106754248A (en) * | 2016-12-27 | 2017-05-31 | 重庆迪赛生物工程有限公司 | A kind of anaerobic culture device |
| CN107794223A (en) * | 2017-11-07 | 2018-03-13 | 广东药科大学附属第医院 | Anaerobic bacteria and the in vitro study model and method of aerobic cell interaction in cell co-culture device and analogue body |
| CN109825425A (en) * | 2019-02-19 | 2019-05-31 | 江苏中新医药有限公司 | A kind of air-tightness culture dish of generation and maintenance anaerobic or micro- oxygen microenvironment |
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| Publication number | Publication date |
|---|---|
| CN102358881B (en) | 2013-01-16 |
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Owner name: CHENGDU RICH SCIENCE INDUSTRY CO., LTD. Free format text: FORMER NAME: CHENGDU RUIQI SCIENCE AND TECHNOLOGY INDUSTRIAL CO., LTD. |
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Address after: 610000 Sichuan city of Chengdu province high tech Zone (West) TIANQIN Street No. 66 Patentee after: CHENGDU RICH SCIENCE INDUSTRY CO., LTD. Address before: 610000, No. 21, West core road, hi tech West District, Sichuan, Chengdu Patentee before: Chengdu Ruiqi Science and Technology Industrial Co., Ltd. |
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