CN102351321B - Composite biological enzyme preparation for deweighting treatment of domestic sludge, and production and application method thereof - Google Patents
Composite biological enzyme preparation for deweighting treatment of domestic sludge, and production and application method thereof Download PDFInfo
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- CN102351321B CN102351321B CN 201110262277 CN201110262277A CN102351321B CN 102351321 B CN102351321 B CN 102351321B CN 201110262277 CN201110262277 CN 201110262277 CN 201110262277 A CN201110262277 A CN 201110262277A CN 102351321 B CN102351321 B CN 102351321B
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Abstract
The invention relates to a composite biological enzyme preparation for the deweighting treatment of domestic sludge, and a production and application method thereof. The invention is characterized in that 100-500 kilograms of composite biological enzyme for the deweighting treatment of domestic sludge are used for 100 thousand tons of municipal domestic sewage, wherein the composite biological enzyme for the deweighting treatment of domestic sludge comprises 25-30 parts of protease, 20-25 parts of one or more of laccase, polyphenol oxidase and manganese peroxidase, 17-21 parts of one or more of xylanase, pentosanase and mannase, 20-25 parts of one or more of cellulase, pectinase and hemicellulase, 10-15 parts of lipase and 12-16 parts of amylase. Compared with the prior art, the invention removes hydrogen sulfide gas and undesirable odor thereof, simultaneously eliminates the inhibiting effect of H2S on the nitrification process, effectively reduces nitrate and ammonia, decomposes proteins, starch, fat, food oil, xylan, cellulose and other organic substances, converts suspended substances, accumulated waste and soluble organic substances in the sludge into carbon dioxide, nitrogengas, oxygen gas, water and sulphate, reduces the TSS (Total Suspended Solids), COD (Chemical Oxygen Demand) and BOD (Biochemical Oxygen Demand), greatly decreases the yield of the sludge, and has the advantages of no poison, no corrosiveness and no secondary pollution production.
Description
Technical field
The present invention relates to a kind of processing domestic sludge preparation and using method thereof.
Background technology
Also do not find at present relevant patent and be and utilize specially that compound biological enzyme is processed the domestic sludge decrement and study, but there is the following several method of document demonstration can realize the decrement of mud, promote organic decomposition in the mud, help the appearance that subtracts of mud, objectively can realize the decrement of mud.
Reference: " the mud decrement new technology in the sewage disposal ", " Multifunctional Compound Microorganism Product is used for the research of mud decrement ", " additional enzyme is strengthened the research of excess sludge hydrolysis ", " Residual Sludge Reduction Technology Progress ", " additional enzyme is strengthened the research of excess sludge hydrolysis under the anaerobic condition ", " sewage plant sludge enzymolysis decrement and resource technology research ".
(1) chemical agent Decrement Technique Tongji University pollutes a kind of new technology of developing with Resource analysis National Key Laboratory, has finished at present the lab scale experimental study.This technology adds several milligrams of chemical agents by every premium on currency in water treatment process, has reduced sludge yield, thereby makes surplus sludge volume reduce 70%～80%.
This technology is in the lab scale experimental study, the decrement of mud is respond well, but when pilot scale and mud handling capacity are larger, the amount of chemical agent will increase gradually, if the words that the residue chemical agent is not handled well, the problem of secondary pollution will be inevitable, so the processing costs of residual pharmacy also will be increased along with the consumption of medicament and increase.In addition, from the angle of history, the life-time service of chemical agent can cause acceptor unavoidably to the immunity of this kind chemical agent, even can cause transgenation of microorganism etc., and all terrible consequences can't help making us halting.Therefore chemical agent use along with the raising of the progress of society, living standards of the people and through the reference to history, the attitude that the various places people are all kept gradually to the use of chemical agent avoidablely all wishes to avoid as far as possible.(2) principle of ozone sludge Decrement Technique ozone sludge Decrement Technique is to set up the ozone oxidation groove in the conventional activated sludge process treatment process, utilizes the effect of ozone that the returned sluge in the ozone oxidation groove is liquefied, with the reduce mud amount.Liquefied substance returns the matrix that the aeration tank becomes microorganism, and the part mineralising, makes the surplus sludge volume in the whole treatment system obtain cutting down.The sewage quantity that the Shima sewage work of Japan processes is 450m3/d, be 0.034kg/kgSS for eliminating the required ozone dosage of residual active sludge fully, and need sludge back flow quantity to be processed is 4 times of conventional sewage treatment plant residual sludge quantity, using ozone technology operation 9 months produces without excess sludge.Facts have proved, mud reduction and ozone add dosage and processed sludge quantity proportional.The Shanghai Municipal Engineering Design and Research Institute adopts Austrian technology, carried out the pilot scale research of ozone oxidation reduce mud amount in Shanghai White dragon hilllock sewage work, ozone is added in the anaerobic digester, ozone can play the effect with the organism cell wall breaking, thereby improves organic matter decomposition rate, increase factor of created gase.
But, this technology lab scale Treating Municipal Sewage effect is fine, chemical engineering sewage is processed in pilot scale, processing cost increases to some extent, major cause is that the ozone of 1 kilogram of at present generation need consume the electricity of degree more than 20, along with the use of ozonizer increases, when increasing cost of investment, the generation expense of ozonizer is also higher.So if the generation technique of ozone has breakthrough, the industrialization of this technology just has practical significance.(3) the reducing biological sludge technology is utilized microfauna that mud is carried out decrement treatment can to set about from following three aspects: the one, utilize the predation of microfauna food chain; The 2nd, directly utilize microfauna to the ingesting and digest of mud, in the reduce mud capacity, increase the solubility of mud; The 3rd, utilize microfauna to strengthen the active of bacterium or increase the quantity of activated bacterium, thereby strengthen autoxidation and the metabolic capacity of bacterium.
Wherein to add biological enzyme as example, make that the anaerobism population becomes aerobic population in the sewage, produce carbonic acid gas, rather than hydrogen sulfide, the simultaneously effect of enzyme is to promote the macromolecular cpd decomposition in the sewage to become micromolecular compound, discharge in conjunction with oxygen, these simple compounds are utilized by multiple-microorganism easily, this also helps the diversity of bacterium, and can improve activity and the fecundity of bacterium, and be conducive to form a large amount of higher organisms, and can promote a large amount of propagation of high photosynthesis organism, provide a large amount of dissolved oxygens for sewage again thus.In the process of these biochemical actions, because having sufficient external source small organic molecule to utilize for microorganism absorbs, therefore this establishment the effect of microorganism endogenous respiration, add increasing of dissolved oxygen, reduce the generation of the foul smell such as the ammonia of microorganism in the anaerobic respiration process, hydrogen sulfide, the substitute is the harmless chemicals of small molecules such as nitrogen, carbonic acid gas and water.Existing many biological treatment systems have been used the method that adds enzyme in the U.S., control efficient and the mud decrement of stink, process with this, such as 6000 tons of domestic sewage factories of Ohio, USA North Royalton.China is utilizing biological method few to the research and comparison of mud decrement, especially utilize the research of compound bio enzyme catalysis mud decrement, because the production process of biological enzyme was complicated in the past, cost is higher, and require the comparison of ingredients of sewage single, be difficult to satisfy the requirement of extensive sewage work, so the work that this respect is carried out just still less.But a large feature of reducing biological sludge is exactly that its safety performance is higher, can not produce secondary pollution, so its researching value is subject to the favorable comment of brainstrust always.At present carrying out the laboratory study of this aspect with regard to Tsing-Hua University, Ningbo Greenwich Environmental Protection Technology Co., Ltd.
Summary of the invention
The purpose of invention of the present invention is to provide a kind of and larger molecular organics in the sewage mainly can be comprised: protein, carbohydrate, the material catalyzed degradations such as fat and PAH high pollution thing become micromolecular monose, amino acid, the compound biological enzyme of the small organic molecule that lipid acid and acetic acid etc. are easily decomposed by microorganism, through a series of catalyzed degradation and biological respinse effect, with the outstanding organic floating thing in the sewage, dissolved organic matter in biomass cells relic and the mud is converted into carbonic acid gas, nitrogen, oxygen, water and vitriol, and can effectively reduce the ammonia of microorganism in the anaerobic respiration process, the generation of the foul smell such as hydrogen sulfide, can not produce composite biological enzyme preparation for deweighting treatment of domestic sludge and the production of secondary pollution, using method.
Composite biological enzyme preparation for deweighting treatment of domestic sludge of the present invention is achieved in that and comprises:
Proteolytic enzyme 25-30 part;
A kind of or above 20-25 part in laccase, polyphenoloxidase, the manganese peroxidase;
A kind of or above 17-21 part in zytase, pentosanase, the mannase;
A kind of or above 20-25 part in cellulase, polygalacturonase, the hemicellulase;
Lipase 10-15 part;
Amylase 12-16 part.
Here, the physical aspect of preparation is solid or solution.
Here, in order to prevent the impact of various biological enzyme solution when storing and use, also be added with composite protectant, composite protectant comprises one or more in glycerine, propylene glycol, the N.F,USP MANNITOL;
In yellow collagen, gelatin, the Xylo-Mucine one or more;
In potassium sorbate, the Sodium Benzoate one or more;
Calcium chloride, sodium-chlor;
Sulfothiorine;
Wherein, the content of one or more in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 2-4wt% of the total amount of all enzymes, the content of one or more in yellow collagen, gelatin, the Xylo-Mucine is the 0.4-0.6wt% of the total amount of all enzymes, one or more content is the 0.01-0.03wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of one or more in calcium chloride, the sodium-chlor is the 1-3wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.01-0.02wt% of the total amount of all enzymes.
The using method of composite biological enzyme preparation for deweighting treatment of domestic sludge of the present invention is achieved in that
100,000 tons of city domestic sewages
Decrement treatment domestic sludge of the present invention compound biological enzyme 100-500 kilogram;
Composite biological enzyme preparation for deweighting treatment of domestic sludge comprises:
Proteolytic enzyme 25-30 part;
A kind of or above 20-25 part in laccase, polyphenoloxidase, the manganese peroxidase;
A kind of or above 17-21 part in zytase, pentosanase, the mannase;
A kind of or above 20-25 part in cellulase, polygalacturonase, the hemicellulase;
Lipase 10-15 part;
Amylase 12-16 part.
Above-mentioned biological enzyme is to throw in the city's sanitary sewage of going down town with the form that the form of mixing solutions preparation or solid list enzyme are thrown in successively.
Here, when above-mentioned biological enzyme is thrown in the city's sanitary sewage of going down town with the form of mixed preparation, contain composite protectant in the preparation, composite protectant comprises one or more in glycerine, propylene glycol, the N.F,USP MANNITOL;
In yellow collagen, gelatin, the Xylo-Mucine one or more;
In potassium sorbate, the Sodium Benzoate one or more;
In calcium chloride, the sodium-chlor one or more;
Sulfothiorine;
Wherein, the content of one or more in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 2-4wt% of the total amount of all enzymes, the content of one or more in yellow collagen, gelatin, the Xylo-Mucine is the 0.4-0.6 wt % of the total amount of all enzymes, one or more content is the 0.01-0.03wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of one or more in calcium chloride, the sodium-chlor is the 1-3wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.01-0.02wt% of the total amount of all enzymes.
The production method of above-mentioned biological enzyme comprises the collection leaching process of bacterial classification successively, the culturing process of bacterial classification, fermenting process, the separation and Extraction process of biological enzyme, the collection leaching process of bacterial classification is to adopt bacterium from domestic sludge, then adopt corresponding substratum to cultivate, to obtain to produce the required bacterial classification of corresponding biological enzyme, then corresponding bacterial classification is mixed with corresponding fermented product and ferment, the separation and Extraction process of biological enzyme is physical sepn leaching process or chemical separation leaching process, the physical sepn leaching process is by centrifugal method fermented product to be carried out centrifugation, the liquid of separating is exactly biological enzyme solution, the chemical separation leaching process is to add ammonium sulfate to saltout in fermented product, then filtering, is exactly the solid biologic enzyme after the fixed substance drying of acquisition.
Here, the solid waste that the physical sepn leaching process produces can be used as feed, and the liquid waste that the chemical separation leaching process produces can be used as fertilizer.
For the various biological enzyme solution that extract with physical method; it is placed to 20-25 ℃; then with laccase; polyphenoloxidase; a kind of in the manganese peroxidase or more than; zytase; pentosanase; a kind of in the mannase or more than; cellulase; polygalacturonase; a kind of in the hemicellulase or more than; lipase; the plurality of enzymes such as amylase; and glycerine; propylene glycol; in three kinds in the N.F,USP MANNITOL one or more; yellow collagen; gelatin; in the Xylo-Mucine one or more; potassium sorbate; in the Sodium Benzoate one or more; calcium chloride; in the sodium-chlor one or more; the composite protectants such as Sulfothiorine mix; and at the uniform velocity stirred 10-15 minute with 20-30 rev/min; add at last protein enzyme solution, at the uniform velocity stirred 5 minutes with 20-30 rev/min, the compound bio zymin can be finished.
And for the solid biologic enzyme that extracts with chemical process, its objective is the transportation for convenient way far away, so we store the various biological enzymes of different ratios respectively, render in the treating pond successively with single enzyme form to the scene.
The quantity discharged of urban industry waste water and sanitary sewage is increasing, and in the treating processes of municipal effluent, certainly leads to a large amount of mud.At present, China's sludge yield is huge, but most mud does not obtain appropriate disposal, be can not be ignored by the mud caused secondary pollution problem that is not effectively addressed, to a certain extent even offset the achievement of pollution emission reduction.The composition of mud is very complicated, zoogloea and the organism of absorption and the aggregate that inorganics forms that it is formed by multiple-microorganism, except containing a large amount of moisture, also contain the organism of difficult degradation and salt and a small amount of pathogenic micro-organism and parasitic ovum etc., heavy metal in addition, contains plurality of heavy metal in the municipal sludge, owing to can not be decomposed by microorganism, and in vivo enrichment, larger to the harm of ecotope.
The present invention is applied in the sewage disposal, purpose is to be degraded into small organic molecule by compound bio enzyme catalysis larger molecular organics, enhanced biological reactivity, thereby minimizing, innoxious, the stabilization of realization mud are for the resource utilization of mud is laid a good foundation.Small organic molecule and the residual body of microorganism that the emphasis of this project utilizes the material catalyzed degradations such as compound bio enzymatic degradation protein, carbohydrate, fat and PAH high pollution thing to become micromolecular monose, amino acid, lipid acid and acetic acid etc. easily to be decomposed by microorganism, thereby the decomposition of organic decrement, PAH high pollution material in the realization domestic sludge, so domestic sludge also reaches minimizing.In addition, the cracking of colloidal structure in the mud, last mud subtracts appearance " weight reducing " because of what the raising of its water separation capability realized mud.
The cardinal principle of present technique is as scalpel with one or more of laccase, polyphenoloxidase and manganese peroxidase, the macromole difficult degradation toxic substances such as catalyzed degradation polycyclic aromatic hydrocarbons, with proteolytic enzyme, amylase, lipase, one or more of Mierocrystalline cellulose, hemicellulase and polygalacturonase, protein, carbohydrate, fat in the enzymatic degradation mud such as one or more in zytase, pentosanase and the mannase.
In other words, it is exactly the katalysis by the coordination of various biological enzymes, macromolecule organic and the residual body of microorganism cells in the degraded mud, thereby the decomposition of the materials such as organic decrement, PAH in the realization domestic sludge, last mud subtract appearance " weight reducing " because of what the raising of its water separation capability realized mud.
Its composite protectant then is to select in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL one or more, one or more of yellow collagen, gelatin, Xylo-Mucine, in calcium chloride, the sodium-chlor one or more can make compound biological enzyme keep easily its rock steady structure in water molecules, and volatility does not lose activity; Select in potassium sorbate, the Sodium Benzoate one or more, effectively control colony number, prevent the rotten of enzyme, be convenient to simultaneously satisfy the requirement of food condition; Select Sulfothiorine, prevent that the oxidation of compound biological enzyme from losing activity.Each raw material among the present invention is beneficial to be obtained, and can keep at ambient temperature the compound bio enzyme activity more than 80% in 6 months, has saved as keeping enzyme activity need to build the cost of freezer, has made things convenient for preservation and the transportation of enzyme.
The present invention compared with the prior art, have the hydrogen sulfide of removing and stink thereof, eliminate simultaneously H2S to the restraining effect of nitrifying process, effectively reduce nitrate and ammonia, the organism such as decomposing protein, starch, fat, food oil, xylan and Mierocrystalline cellulose, soluble organic in suspended substance, accumulation refuse and the mud is converted into carbonic acid gas, nitrogen, oxygen, water and vitriol, reduce TSS, COD and BOD, greatly reduce the output of mud, and nontoxic, non-corrosiveness can not produce the advantage of secondary pollution.
Embodiment:
Now in conjunction with the embodiments the present invention is done and describes in further detail:
Composite biological enzyme preparation for deweighting treatment of domestic sludge of the present invention is achieved in that and comprises:
Proteolytic enzyme 25-30 part;
A kind of or above 20-25 part in laccase, polyphenoloxidase, the manganese peroxidase;
A kind of or above 17-21 part in zytase, pentosanase, the mannase;
A kind of or above 20-25 part in cellulase, polygalacturonase, the hemicellulase;
Lipase 10-15 part;
Amylase 12-16 part.
Here, the physical aspect of preparation is solid or solution.
Also be added with composite protectant in the solution, composite protectant comprises one or more in glycerine, propylene glycol, the N.F,USP MANNITOL;
In yellow collagen, gelatin, the Xylo-Mucine one or more;
In potassium sorbate, the Sodium Benzoate one or more;
In calcium chloride, the sodium-chlor one or more;
Sulfothiorine;
Wherein, the content of one or more in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 2-4wt% of the total amount of all enzymes, the content of one or more in yellow collagen, gelatin, the Xylo-Mucine is the 0.4-0.6 wt % of the total amount of all enzymes, one or more content is the 0.01-0.03wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of one or more in calcium chloride, the sodium-chlor is the 1-3wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.01-0.02wt% of the total amount of all enzymes.
The using method of composite biological enzyme preparation for deweighting treatment of domestic sludge of the present invention is achieved in that
100,000 tons of city domestic sewages
Decrement treatment domestic sludge biological enzyme 100-500 kilogram;
Decrement treatment domestic sludge biological enzyme comprises:
Proteolytic enzyme 25-30 part;
A kind of or above 20-25 part in laccase, polyphenoloxidase, the manganese peroxidase;
A kind of or above 17-21 part in zytase, pentosanase, the mannase;
A kind of or above 20-25 part in cellulase, polygalacturonase, the hemicellulase;
Lipase 10-15 part;
Amylase 12-16 part.
Here, when above-mentioned biological enzyme is thrown in the city's sanitary sewage of going down town with the form of mixed preparation, contain composite protectant in the preparation, composite protectant comprises one or more in glycerine, propylene glycol, the N.F,USP MANNITOL;
In yellow collagen, gelatin, the Xylo-Mucine one or more;
In potassium sorbate, the Sodium Benzoate one or more;
In calcium chloride, the sodium-chlor one or more;
Sulfothiorine;
Wherein, the content of one or more in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 2-4wt% of the total amount of all enzymes, the content of one or more in yellow collagen, gelatin, the Xylo-Mucine is the 0.4-0.6 wt % of the total amount of all enzymes, one or more content is the 0.01-0.03wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of one or more in calcium chloride, the sodium-chlor is the 1-3wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.01-0.02wt% of the total amount of all enzymes.
The concrete use as described in the following example:
Certain city domestic sewage factory treatment process is activated sludge process, process section comprises grid section, preliminary sedimentation tank, biochemistry pool (anaerobic pond, aeration tank), second pond, returned sluge section, the additive of compound enzymic preparation generally is to add in anaerobic pond, aeration tank, second pond one or more, and this is to decide according to the pond water treatment situation of each process section.
Embodiment 1:
Certain city domestic sewage factory treatment process is activated sludge process, process section comprises grid section, preliminary sedimentation tank, biochemistry pool (anaerobic pond, aeration tank), second pond, returned sluge section, the additive of compound enzymic preparation is to add in anaerobic pond, aeration tank, second pond, wherein
(1) in processing, adds anaerobic pond:
25 parts in proteolytic enzyme;
22 parts of laccases;
17 parts of zytase, pentosanases;
25 parts of cellulase, hemicellulases;
15 parts in lipase;
12 parts of amylase.
Composite protectant: the 5.025wt% of the total amount of all enzymes, wherein:
The 3wt% of the total amount of all enzymes of propylene glycol,
The 0.5wt% of the total amount of yellow all enzymes of collagen
The 0.01wt% of the total amount of all enzymes of potassium sorbate
The 1.5wt% of the total amount of calcium chloride, all enzymes of sodium-chlor
The 0.015wt% of the total amount of all enzymes of Sulfothiorine
(2) in processing, adds Aerobic Pond:
28 parts in proteolytic enzyme;
21 parts of laccase, polyphenoloxidase;
19 parts of zytase, pentosanases;
23 parts of cellulases;
10 parts in lipase;
12 parts of amylase.
Composite protectant: the 5.64wt% of the total amount of all enzymes, wherein:
The 4wt% of the total amount of all enzymes of glycerine
The 0.6wt% of the total amount of yellow all enzymes of collagen
The 0.02wt% of the total amount of all enzymes of Sodium Benzoate
The 1wt% of the total amount of all enzymes of calcium chloride
The 0.02wt% of the total amount of all enzymes of Sulfothiorine
(3) in processing, adds second pond::
0 part of protease 3;
20 parts of laccases;
21 parts of zytase, pentosanases;
21 parts of polygalacturonase, hemicellulases;
11 parts in lipase;
14 parts of amylase.
Composite protectant: the 5.75wt% of the total amount of all enzymes, wherein:
The 4wt% of the total amount of all enzymes of glycerine
0. 6wt% of the total amount of all enzymes of gelatin
The 0.03wt% of the total amount of all enzymes of potassium sorbate
The 2wt% of the total amount of calcium chloride, all enzymes of sodium-chlor
The 0.02wt% of the total amount of all enzymes of Sulfothiorine
Embodiment 2:
Certain city domestic sewage factory treatment process is activated sludge process, and process section comprises grid section, preliminary sedimentation tank, biochemistry pool (anaerobic pond, aeration tank), second pond, returned sluge section, and the additive of compound enzymic preparation is to add in anaerobic pond, wherein
(1) in processing, adds anaerobic pond:
0 part of protease 3;
Laccase, manganese peroxidase 25 part;
17 parts of zytase, pentosanases;
23 parts of polygalacturonase, hemicellulases;
13 parts in lipase;
14 parts of amylase
Composite protectant: the 6.44wt% of the total amount of all enzymes, wherein:
3 wt% of the total amount of all enzymes of propylene glycol
The 0.4wt% of the total amount of yellow all enzymes of collagen
The 0.02wt% of the total amount of all enzymes of potassium sorbate
The 2wt% of the total amount of calcium chloride, all enzymes of sodium-chlor
The 0.02wt% of the total amount of all enzymes of Sulfothiorine
Embodiment 3:
Certain city domestic sewage factory treatment process is activated sludge process, and process section comprises grid section, preliminary sedimentation tank, biochemistry pool (anaerobic pond, aeration tank), second pond, returned sluge section, and the additive of compound enzymic preparation is to add in the aeration tank, wherein
(1) in processing, adds Aerobic Pond:
25 parts in proteolytic enzyme;
Manganese peroxidase 25 part;
17 parts of zytase, mannases;
25 parts of cellulases;
13 parts in lipase;
Composite protectant: the 5.64wt% of the total amount of all enzymes, wherein:
The 4wt% of the total amount of all enzymes of glycerine
The 0.6wt% of the total amount of yellow all enzymes of collagen
The 0.03wt% of the total amount of all enzymes of Sodium Benzoate
The 3wt% of the total amount of all enzymes of sodium-chlor
The 0.02wt% of the total amount of all enzymes of Sulfothiorine
Embodiment 4:
Certain city domestic sewage factory treatment process is activated sludge process, and process section comprises grid section, preliminary sedimentation tank, biochemistry pool (anaerobic pond, aeration tank), second pond, returned sluge section, and the additive of compound enzymic preparation is to add in second pond, wherein
(1) in processing, adds second pond::
0 part of protease 3;
20 parts of laccases;
21 parts of zytase, mannases;
20 parts of cellulases;
16 parts in lipase;
15 parts of amylase
Composite protectant: the 7.22wt% of the total amount of all enzymes, wherein:
The 3.5wt% of the total amount of all enzymes of glycerine
The 0.6wt% of the total amount of all enzymes of gelatin
The 0.02wt% of the total amount of all enzymes of potassium sorbate
The 3wt% of the total amount of calcium chloride, all enzymes of sodium-chlor
The 0.015wt% of the total amount of all enzymes of Sulfothiorine
Embodiment 5
The compound biological enzyme prescription is set as the prescription of 3 large types, and the ratio of every kind of contained enzyme of prescription is specially:
Prescription 1: proteolytic enzyme: 25-27 part
A kind of or above 20-22 part in laccase, polyphenoloxidase, the manganese peroxidase;
A kind of or above 17-19 part in zytase, pentosanase, the mannase;
A kind of or above 20-22 part in cellulase, polygalacturonase, the hemicellulase;
Lipase 10-12 part;
Amylase 12-14 part.
Here, the physical aspect of preparation is solid or solution, also is added with composite protectant,
Wherein, the content of one or more in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 2-3wt% of the total amount of all enzymes, the content of one or more in yellow collagen, gelatin, the Xylo-Mucine is the 0.4-0.5 wt % of the total amount of all enzymes, one or more content is the 0.01-0.02wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of one or more in calcium chloride, the sodium-chlor is the 1-2wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.01-0.015wt% of the total amount of all enzymes.
Prescription 2: proteolytic enzyme: 26-28 part
A kind of or above 22-24 part in laccase, polyphenoloxidase, the manganese peroxidase;
A kind of or above 18-20 part in zytase, pentosanase, the mannase;
A kind of or above 22-24 part in cellulase, polygalacturonase, the hemicellulase;
Lipase 12-14 part;
Amylase 14-15 part.
Here, the physical aspect of preparation is solid or solution, also is added with composite protectant,
Wherein, the content of one or more in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 3-4wt% of the total amount of all enzymes, the content of one or more in yellow collagen, gelatin, the Xylo-Mucine is the 0.4-0.5 wt % of the total amount of all enzymes, one or more content is the 0.02-0.025wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of one or more in calcium chloride, the sodium-chlor is the 1.5-2.5wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.01-0.015wt% of the total amount of all enzymes.
Prescription 3: proteolytic enzyme: 28-30 part
A kind of or above 24-25 part in laccase, polyphenoloxidase, the manganese peroxidase;
A kind of or above 20-21 part in zytase, pentosanase, the mannase;
A kind of or above 24-25 part in cellulase, polygalacturonase, the hemicellulase;
Lipase 14-15 part;
Amylase 15-16 part.
Here, the physical aspect of preparation is solid or solution, also is added with composite protectant,
Wherein, the content of one or more in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 3-4wt% of the total amount of all enzymes, the content of one or more in yellow collagen, gelatin, the Xylo-Mucine is the 0.5-0.6 wt % of the total amount of all enzymes, one or more content is the 0.02-0.03wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of one or more in calcium chloride, the sodium-chlor is the 2-3wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.015-0.02wt% of the total amount of all enzymes.
Certain city domestic sewage factory treatment process is activated sludge process, and process section comprises grid section, preliminary sedimentation tank, biochemistry pool (anaerobic pond, aeration tank), second pond, returned sluge section, and the additive of compound enzymic preparation is to add in second pond, wherein
(1) in the Air Exposure pond, add:
Prescription 1: prescription 2: 3 1:2:3 fill a prescription
Be added in the aeration tank simultaneously respectively at different points
(2) in anaerobic treatment pool, add:
Prescription 1: prescription 2: 3 2:1:4 fill a prescription
Be added in the anaerobic pond simultaneously respectively at different points
(3) in two heavy treating ponds, add:
Prescription 1: prescription 2: 3 3:1:1 fill a prescription
Be added in the second pond simultaneously respectively at different points
The production method of above-mentioned biological enzyme comprises the collection leaching process of bacterial classification successively, the culturing process of bacterial classification, fermenting process, the separation and Extraction process of biological enzyme, the collection leaching process of bacterial classification is to adopt bacterium from domestic sludge, then adopt corresponding substratum to cultivate, to obtain to produce the required bacterial classification of corresponding biological enzyme, then corresponding bacterial classification is mixed with corresponding fermentation bed material and ferment, the separation and Extraction process of biological enzyme is physical sepn leaching process or chemical separation leaching process, the physical sepn leaching process is by centrifugal method fermented product to be carried out centrifugation, the liquid of separating is exactly biological enzyme solution, the chemical separation leaching process is to add ammonium sulfate to saltout in fermented product, then filtering, is exactly the solid biologic enzyme after the fixed substance drying of acquisition.
Here, the solid waste that the physical sepn leaching process produces can be used as feed, and the liquid waste that the chemical separation leaching process produces can be used as fertilizer.
Here; to extract the various biological enzyme solution of separating from physical method first and be placed to 20-25 ℃; then with laccase; polyphenoloxidase; a kind of in the manganese peroxidase or more than; zytase; pentosanase; a kind of in the mannase or more than; cellulase; polygalacturonase; a kind of in the hemicellulase or more than; lipase; the plurality of enzymes such as amylase; and glycerine; propylene glycol; in three kinds in the N.F,USP MANNITOL one or more; yellow collagen; gelatin; in the Xylo-Mucine one or more; potassium sorbate; in the Sodium Benzoate one or more; calcium chloride; in the sodium-chlor one or more; the composite protectants such as Sulfothiorine mix; and at the uniform velocity stirred 10-15 minute with 20-30 rev/min; add at last protein enzyme solution, at the uniform velocity stirred 5 minutes with 20-30 rev/min, the compound bio zymin can be finished.
And for the solid biologic enzyme that extracts with chemical process, its objective is the transportation for convenient way far away, so we store the various biological enzymes of different ratios respectively, render in the treating pond successively with single enzyme form to the scene.
Claims (6)
1. a composite biological enzyme preparation for deweighting treatment of domestic sludge is characterized in that, comprising:
Proteolytic enzyme 25-30 part;
More than one 20-25 parts in laccase, polyphenoloxidase, the manganese peroxidase;
More than one 17-21 parts in zytase, pentosanase, the mannase;
More than one 20-25 parts in cellulase, polygalacturonase, the hemicellulase;
Lipase 10-15 part;
Amylase 12-16 part.
2. composite biological enzyme preparation for deweighting treatment of domestic sludge according to claim 1, the physical aspect that it is characterized in that preparation is solid or solution.
3. composite biological enzyme preparation for deweighting treatment of domestic sludge according to claim 1 and 2 is characterized in that containing composite protectant, and composite protectant comprises more than one in glycerine, propylene glycol, the N.F,USP MANNITOL;
In yellow collagen, gelatin, the Xylo-Mucine more than one;
In potassium sorbate, the Sodium Benzoate more than one;
In calcium chloride, the sodium-chlor more than one;
Sulfothiorine;
Wherein, the content of more than one in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 2-4wt% of the total amount of all enzymes, the content of more than one in yellow collagen, gelatin, the Xylo-Mucine is the 0.4-0.6wt% of the total amount of all enzymes, more than one content is the 0.01-0.03wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of more than one in calcium chloride, the sodium-chlor is the 1-3wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.01-0.02wt% of the total amount of all enzymes.
4. the using method of a composite biological enzyme preparation for deweighting treatment of domestic sludge is characterized in that
100,000 tons of city domestic sewages
Decrement treatment domestic sludge compound biological enzyme 100-500 kilogram;
Decrement treatment domestic sludge compound biological enzyme comprises:
Proteolytic enzyme 25-30 part;
More than one 20-25 parts in laccase, polyphenoloxidase, the manganese peroxidase;
More than one 17-21 parts in zytase, pentosanase, the mannase;
More than one 20-25 parts in cellulase, polygalacturonase, the hemicellulase;
Lipase 10-15 part;
Amylase 12-16 part;
Above-mentioned biological enzyme is to throw in the city's sanitary sewage of going down town with the form of mixed preparation or with the form that single kind enzyme is thrown in successively.
5. the using method of composite biological enzyme preparation for deweighting treatment of domestic sludge according to claim 4, when it is characterized in that multiple biological enzyme is thrown in the city's sanitary sewage of going down town with the form of mixed preparation, contain composite protectant in the preparation, composite protectant comprises more than one in glycerine, propylene glycol, the N.F,USP MANNITOL;
In yellow collagen, gelatin, the Xylo-Mucine more than one;
In potassium sorbate, the Sodium Benzoate more than one;
In calcium chloride, the sodium-chlor more than one;
Sulfothiorine;
Wherein, the content of more than one in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 2-4wt% of the total amount of all enzymes, the content of more than one in yellow collagen, gelatin, the Xylo-Mucine is the 0.4-0.6wt% of the total amount of all enzymes, more than one content is the 0.01-0.03wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of more than one in calcium chloride, the sodium-chlor is the 1-3wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.01-0.02% wt of the total amount of all enzymes.
6. the using method of composite biological enzyme preparation for deweighting treatment of domestic sludge according to claim 4, it is characterized in that compound biological enzyme plants form that enzyme throws in successively when throwing in the city's sanitary sewage of going down town with single, throw in simultaneously composite protectant, composite protectant comprises more than one in glycerine, propylene glycol, the N.F,USP MANNITOL;
In yellow collagen, gelatin, the Xylo-Mucine more than one;
In potassium sorbate, the Sodium Benzoate more than one;
In calcium chloride, the sodium-chlor more than one;
Sulfothiorine;
Wherein, the content of more than one in three kinds of the glycerine, propylene glycol, N.F,USP MANNITOL is the 2-4wt% of the total amount of all enzymes, the content of more than one in yellow collagen, gelatin, the Xylo-Mucine is the 0.4-0.6wt% of the total amount of all enzymes, more than one content is the 0.01-0.03wt% of the total amount of all enzymes in potassium sorbate, the Sodium Benzoate, the content of more than one in calcium chloride, the sodium-chlor is the 1-3wt% of the total amount of all enzymes, and the content of Sulfothiorine is the 0.01-0.02% wt of the total amount of all enzymes.
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| CN109502764A (en) * | 2018-12-25 | 2019-03-22 | 山东华泰纸业股份有限公司 | A kind of biological enzyme formulation and the method using its raising paper-making industry composite waste treatment effeciency |
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| JP2003260491A (en) * | 2002-03-08 | 2003-09-16 | Ebara Corp | Biological treatment method for organic sewage and apparatus therefor |
| JP4034705B2 (en) * | 2003-08-27 | 2008-01-16 | 株式会社神鋼環境ソリューション | Novel microorganism and method for treating organic solid using the microorganism |
| CN101898830B (en) * | 2010-05-05 | 2011-12-28 | 麦广鸿 | Complex enzyme preparation for sewage treatment and application method thereof |
| CN102010109B (en) * | 2010-10-29 | 2012-09-19 | 孙祥章 | Application of bio-enzyme catalysis in residual sludge treatment |
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