CN116355882B - Biological enzyme preparation for controlling filamentous fungus expanded sludge and preparation method and application thereof - Google Patents
Biological enzyme preparation for controlling filamentous fungus expanded sludge and preparation method and application thereof Download PDFInfo
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- CN116355882B CN116355882B CN202310370265.XA CN202310370265A CN116355882B CN 116355882 B CN116355882 B CN 116355882B CN 202310370265 A CN202310370265 A CN 202310370265A CN 116355882 B CN116355882 B CN 116355882B
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- 239000010802 sludge Substances 0.000 title claims abstract description 105
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 86
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 86
- 238000002360 preparation method Methods 0.000 title claims abstract description 68
- 241000233866 Fungi Species 0.000 title claims abstract description 35
- 229940088598 enzyme Drugs 0.000 claims abstract description 90
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims abstract description 34
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 34
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 34
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910001629 magnesium chloride Inorganic materials 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 17
- 229910000160 potassium phosphate Inorganic materials 0.000 claims abstract description 17
- 235000011009 potassium phosphates Nutrition 0.000 claims abstract description 17
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 17
- 108010002430 hemicellulase Proteins 0.000 claims abstract description 12
- 235000012239 silicon dioxide Nutrition 0.000 claims abstract description 12
- 108010065511 Amylases Proteins 0.000 claims abstract description 11
- 102000013142 Amylases Human genes 0.000 claims abstract description 11
- 108010059892 Cellulase Proteins 0.000 claims abstract description 11
- 235000019418 amylase Nutrition 0.000 claims abstract description 11
- 239000004382 Amylase Substances 0.000 claims abstract description 10
- 108091005804 Peptidases Proteins 0.000 claims abstract description 10
- 239000004365 Protease Substances 0.000 claims abstract description 10
- 229940106157 cellulase Drugs 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 9
- 229940059442 hemicellulase Drugs 0.000 claims abstract description 9
- 238000004108 freeze drying Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000002994 raw material Substances 0.000 claims description 10
- 238000005303 weighing Methods 0.000 claims description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 108010084185 Cellulases Proteins 0.000 claims description 5
- 102000005575 Cellulases Human genes 0.000 claims description 5
- 239000011812 mixed powder Substances 0.000 claims description 5
- 239000003223 protective agent Substances 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- 239000007983 Tris buffer Substances 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 4
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 238000004806 packaging method and process Methods 0.000 claims description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
- 239000008118 PEG 6000 Substances 0.000 claims description 2
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 claims description 2
- 239000002202 Polyethylene glycol Substances 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 claims description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims description 2
- 230000002538 fungal effect Effects 0.000 claims description 2
- 239000008101 lactose Substances 0.000 claims description 2
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 2
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 2
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 2
- 229920001223 polyethylene glycol Polymers 0.000 claims description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 2
- 229940073490 sodium glutamate Drugs 0.000 claims description 2
- 239000000600 sorbitol Substances 0.000 claims description 2
- 235000010356 sorbitol Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000010865 sewage Substances 0.000 abstract description 24
- 150000001875 compounds Chemical class 0.000 abstract description 13
- 241000894006 Bacteria Species 0.000 abstract description 8
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- 230000004071 biological effect Effects 0.000 abstract description 6
- 238000011084 recovery Methods 0.000 abstract description 3
- 238000004904 shortening Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 7
- 238000004062 sedimentation Methods 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000003197 catalytic effect Effects 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000012868 Overgrowth Diseases 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000008394 flocculating agent Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 101710112457 Exoglucanase Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000004103 aerobic respiration Effects 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000011545 laboratory measurement Methods 0.000 description 1
- 238000012792 lyophilization process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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Abstract
The invention relates to a filamentous fungus expanded sludge control biological enzyme preparation, a preparation method and application thereof, wherein the biological enzyme preparation comprises a biological enzyme compound, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride, and the biological enzyme compound is a mixture of cellulase, hemicellulase, amylase and protease. The biological enzyme preparation is used for treating the expanded sludge in the sewage treatment process, and realizes the purpose of effectively reducing the generation of sludge expansion, shortening the sludge expansion time, protecting the biological activity of dominant bacteria and realizing the rapid recovery of the function of activated sludge on the premise of not damaging the biological activity of filamentous bacteria.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and particularly relates to a filamentous fungus expanded sludge control biological enzyme preparation, a preparation method and application thereof.
Background
Sludge bulking is a common condition in the operation of sewage treatment plants, with high development, prevalence and hazard, and is no exception in anaerobic ammonia oxidation systems. Research shows that about 45% of the existing 100 anaerobic ammonia oxidation projects worldwide face sludge bulking problems. Sludge expansion occurs in the sewage treatment process, so that sludge loss is caused, and the treatment capacity of the whole system can be obviously weakened.
Sludge bulking is mostly caused by filamentous fungus bulking and affects many factors such as environmental conditions, operating conditions and extracellular polymers. Sludge bulking can have a great impact on the biochemical treatment of sewage treatment plants. Most of the sludge bulking phenomenon occurring in sewage treatment plants is caused by overgrowth of filamentous fungi. The expansion caused by the filamentous bacteria can cause unbalance of microorganism population in the activated sludge, thereby reducing the removal efficiency of the activated sludge on organic pollutants, ammonia nitrogen, total nitrogen and phosphorus.
At present, the filamentous fungus expansion control generally adopts a method of a biological selector, adding disinfectant and the like. The essence of these methods is to reduce the abundance of filamentous bacteria, thereby restoring sludge settling properties. However, this approach to impair the ecological balance of filamentous fungi often disrupts the structure of the activated sludge flora, making sludge activity difficult to build up over a long period of time. Most sewage treatment plants optimize sewage treatment effects by changing the water quality of inflow water, adjusting the running mode and other factors, and the sewage expansion is extremely easy to be induced by the water quality characteristics of the sewage in actual running, temperature change caused by seasonal change, low dissolved oxygen and the like in running, so that the difficulty of preventing and controlling the sludge expansion is increased.
Therefore, how to effectively reduce the generation of sludge bulking and shorten the sludge bulking time without damaging the bioactivity of the filamentous fungi is a problem to be solved.
Disclosure of Invention
In order to solve the problem of sludge expansion commonly existing in sewage plants, and simultaneously avoid excessive damage to the main active strain filamentous fungi, so that the treatment capacity is reduced, the use of chemical agents is reduced, the sludge expansion time is shortened, and the sewage treatment effect and degradation efficiency are improved, the invention provides a filamentous fungi expanded sludge control biological enzyme preparation, and a preparation method and application thereof. The biological enzyme preparation is used for treating the expanded sludge in the sewage treatment process, can effectively reduce the generation of sludge expansion, shorten the sludge expansion time, protect the biological activity of dominant bacteria and realize the rapid recovery of the function of activated sludge.
The biological activity of the filamentous fungi can be damaged by adopting a biological selector or a disinfectant adding method in the prior art for controlling the expansion of the filamentous fungi, and the functions of the activated sludge can be influenced. Therefore, the inventor researches the technology, and discovers that the fermentation state and the biological activity of the filamentous bacteria in the biochemical pond are adjusted, so that the microbial sewage treatment capacity is increased, and the technology is an efficient and energy-saving sludge expansion control technology. Over 90% of the activated sludge bulking was found during the study to be caused by overgrowth of the filamentous fungus, and it was necessary to control the fermentation behavior and population numbers that caused the filamentous fungus in the biochemical pond. The composition and structure of the activated sludge microbial community have great influence on the effect of the treatment system and the sedimentation performance of the sludge. With the development and application of molecular biology technology, the research on the overall diversity change of microbial flora and competition relationship among microorganisms in an expanded sludge system becomes more and more abundant, and the possibility is provided for further controlling the occurrence of sludge expansion. Based on this, the inventors have found in research that the addition of biological enzymes to a sludge system can control sludge bulking more effectively than existing flocculants or formulated agents. When further researching what biological enzyme system has the best effect on controlling sludge expansion, the inventor discovers that the biological enzyme compound consisting of four enzymes of cellulase, hemicellulase, amylase and protease is applied to sludge treatment, so that the generation of sludge expansion can be effectively reduced, and the sludge expansion time can be shortened. In view of this, the inventors have provided the following scheme of the present invention.
In order to achieve the above object, a first aspect of the present invention provides a filamentous fungus expanded sludge control biological enzyme preparation comprising a biological enzyme complex, silica, potassium phosphate, ammonium nitrate and magnesium chloride;
the weight portions of the raw materials are as follows:
the biological enzyme complex is a mixture of cellulases, hemicellulases, amylases and proteases.
Further, the biological enzyme compound comprises the following raw materials in parts by weight:
further, the cellulases and hemicellulases are of fungal, bacterial or plant origin. Among them, cellulase is a generic term for enzymes that degrade cellulose to produce glucose. Most cellulases contain a specific structure: the catalytic region and the cellulose binding region are typically joined by a peptide segment. The catalytic region has catalytic sites to assist in cellulose binding. Cellulases can be classified into endoglucanases, exoglucanases and beta-glucosidase.
The second aspect of the invention provides a preparation method of a filamentous fungus expanded sludge control biological enzyme preparation, which comprises the following steps:
step 1, dissolving: dissolving the biological enzyme complex in a solvent, wherein the solvent is one of water, citric acid buffer solution, phosphoric acid buffer solution, tris (Tris) hydrochloric acid solution, polyethylene glycol and polyvinylpyrrolidone in different proportions to obtain an enzyme preparation mixed solution;
step 2, freeze-drying: adding a freeze-drying protective agent into the enzyme preparation mixed solution, and performing freeze-drying treatment to obtain freeze-drying enzyme preparation mixed powder;
and 3, respectively weighing the mixed powder of the freeze-dried enzyme preparation, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride according to the weight parts, and independently packaging and storing to obtain the filamentous fungus expanded sludge controlled biological enzyme preparation.
In step 2, the addition amount of the lyoprotectant is 1% -10% of the mass of the enzyme preparation mixed solution, and the lyoprotectant is one of sucrose, starch, trehalose, lactose, maltose, PEG2000, PEG6000, sodium dihydrogen phosphate, sodium glutamate and sorbitol.
Further, in step 2, the conditions of the lyophilization process are: vacuum degree is 0-10 Pa, temperature is-30 ℃ to-50 ℃, and freeze-drying time is 24-72 h.
The third aspect of the present invention provides a treatment method for controlling filamentous fungi expanded sludge using the biological enzyme preparation, comprising the steps of:
weighing the biological enzyme compound, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride according to the weight parts; adding the obtained biological enzyme preparation into sludge to be treated according to the adding amount of 10 mg/L-30 mg/L.
Compared with the prior art, the invention has the advantages that:
1. the biological enzyme preparation takes a compound composed of four enzymes of cellulase, hemicellulase, amylase and protease as main functional components, and plays roles of degrading complex organic matters and hydrolyzing fiber components in water; and simultaneously, the added silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride respectively play a role in sedimentation.
2. The biological enzyme preparation is used for treating the expanded sludge in the sewage treatment process, can effectively reduce the generation of sludge expansion, shorten the sludge expansion time, protect the biological activity of dominant bacteria and realize the rapid recovery of the function of activated sludge.
Drawings
The invention is further described below with reference to the drawings and examples.
FIG. 1 is a graph showing the SVI value change of activated sludge after adding a biological enzyme preparation in example 5 of the present invention.
Detailed Description
In the following examples, unless otherwise specified, the materials and experimental equipment involved are commercially available.
The raw materials are shown in Table 1.
TABLE 1
The experimental apparatus is shown in Table 2.
TABLE 2
Example 1 preparation of biological enzyme preparation
The embodiment provides a filamentous fungus expanded sludge control biological enzyme preparation, which comprises a biological enzyme compound, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride, wherein the biological enzyme compound is a mixture of cellulase, hemicellulase, amylase and protease. The weight portions of the raw materials are as follows:
firstly, respectively weighing cellulase, hemicellulase, amylase and protease according to the weight parts, mixing to form a biological enzyme compound, weighing 1.0g of the biological enzyme compound, adding 100ml of distilled water, stirring for 30min, adding 3% trehalose as a freeze-drying protective agent, centrifuging at 4 ℃, and storing supernatant in a refrigerator. And adopting a Thermo Scientific freeze dryer to freeze-dry the biological enzyme complex. The conditions of the freeze-drying treatment are as follows: vacuum degree is 0-10 Pa, temperature is-40 ℃, and freeze-drying time is 24h.
And (3) after the freeze-drying is finished, obtaining freeze-dried powder (namely the biological enzyme compound after freeze-drying) of the white solid enzyme mixed preparation, and freezing and preserving at 20 ℃.
And respectively weighing the freeze-dried biological enzyme compound, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride according to the weight parts, and independently packaging and storing to obtain the filamentous fungus expanded sludge controlled biological enzyme preparation. When the biological enzyme preparation is used, all raw materials are shaken and uniformly shaken for use.
Example 2
The filamentous fungus expanded sludge control biological enzyme preparation is prepared by the same method as in the embodiment 1, except that the raw materials are used in different amounts, and the weight parts ratio of the raw materials in the embodiment is as follows:
example 3
The filamentous fungus expanded sludge control biological enzyme preparation is prepared by the same method as in the embodiment 1, except that the raw materials are used in different amounts, and the weight parts ratio of the raw materials in the embodiment is as follows:
EXAMPLE 4 laboratory measurement of control of the swelling of filamentous activated sludge by biological enzyme preparations
In the embodiment, for detecting the control effect of the biological enzyme preparation on the expansion of the filamentous fungi activated sludge, which is obtained in the embodiment 1, an SBR reactor is selected, a water sample is domestic sewage, the test is carried out in a low-temperature control chamber, the water temperature is controlled to be 0-10 ℃, seed sludge is respectively added into each SBR reactor, and the sludge concentration is about 6500 mg/L. After the reactor is operated for 6 days, the sludge begins to expand, the volume index SVI of the sludge is changed from 100-122mL/g to 160-180mL/g, the sludge is kept in an expanded state for a period of time, and the activated sludge is expanded by the filamentous bacteria. As the system is run to 23d, SVI is raised to 225-254mL/g.
Flocculant (10 mg/L), amylase (5-10U/g), lyophilized biological enzyme complex (5-10U/g) as described in example 1, and biological enzyme preparation (10 mg/L) as described in example 1 were added to each SBR reactor. After continuous operation for 5d, the working condition NH is measured 4+ The removal rates of N are 33%, 27%, 30% and 56%, respectively, and the NH of the effluent 4+ N was 1.9, 2.5, 2.1, 0.9mg/L, and the average removal of COD by the 4 SBR reactors was 58.0%, 19.7%, 47.6% and 73.4%, respectively. SVI values were 208, 235, 221, 135mL/g, respectively.
Illustrating that the bio-enzyme formulation of example 1 is effective in controlling sludge bulking over other flocculants or formulary agents.
Example 5 application of biological enzyme preparation to wastewater treatment plant 1
To examine the effect of the biological enzyme preparation prepared in example 2 on the control of the expansion of filamentous fungi activated sludge, the biological enzyme preparation prepared in example 2 was applied to the sludge treatment of municipal sewage plants.
In the running process of the three-section A/O technology of the urban sewage treatment plant, the quality of the forward effluent water generated by expansion is shown in the following table 3. And when the 8 th step is operated, the sludge is expanded, the SVI is 180mL/g, and the sludge sedimentation performance is poor. At this time, the biological enzyme complex, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride are weighed according to the weight portion ratio described in example 2, the mixture is added into the sludge, the adding amount of the biological enzyme preparation is 10mg/L of the sludge, the sedimentation performance of the sludge in 9 th to 12d is gradually recovered, the sedimentation performance of the sludge in 15d is stable, and the SVI is stabilized at 56mL/g. The biological enzyme preparation described in example 2 is added to effectively control sludge bulking.
TABLE 3 quality of the forward effluent after expansion
| Project | COD | NH 4 + -N | PO 4 3- -P |
| Inlet water (mg/L) | 201.32±23.13 | 23.16±7.84 | 1.28±0.74 |
| Effluent (mg/L) | 30.53±9.71 | 1.43±0.14 | 0.13±0.04 |
| Removal rate (%) | 85.01±7.26 | 93.82±2.34 | 89.8±2.93 |
Example 5 application of biological enzyme preparation to wastewater treatment plant 2
To further examine the effect of the biological enzyme preparation prepared in example 2 on the control of the expansion of the filamentous fungi activated sludge, the biological enzyme preparation prepared in example 2 was applied to the sludge treatment of municipal sewage plants.
In the running process of the three-section A/O technology of the urban sewage treatment plant, the SVI value of the activated sludge of the biological reaction tank reaches 181.6mL/g, and the sludge expansion occurs. At this time, the bio-enzyme complex, silica, potassium phosphate, ammonium nitrate and magnesium chloride were weighed according to the weight ratio described in example 2, the mixture was added to the sludge, the amount of the bio-enzyme preparation added was 30mg/L of sludge, and the SVI value of the activated sludge after the bio-enzyme preparation was added was monitored, and the result was shown in FIG. 1. In the operation of 6d, the SV of the sludge is reduced from 67% to 32%, the MLSS is 3.6g/L, the sedimentation performance is obviously improved, the aerobic respiration rate of the sludge is reduced by 20.13%, and the SVI is 125.38mL/g.
The biological enzyme preparation described in example 2 is added to effectively control sludge bulking.
TABLE 4 sludge bulking application example parameters for sewage plants
| Parameters (parameters) | Expansion of | 6d after the biological enzyme preparation is added |
| MLSS(g/L) | 5.9 | 3.6 |
| SVI(mL/g) | 181.6 | 125.38 |
| SV(％) | 67 | 32 |
Example 6 application of biological enzyme preparation to wastewater treatment plant 3
To examine the control effect of the biological enzyme preparation prepared in example 3 on the expansion of filamentous fungi activated sludge, the biological enzyme preparation prepared in example 3 was applied to sludge treatment of municipal sewage plants.
In the running process of the three-section A/O technology of the urban sewage treatment plant, the running condition of the sewage treatment plant is that water is fed for 10min, aeration is carried out for 135min, sedimentation is carried out for 10min, water is discharged for 10min, and the operation is left for 10min and stirred for 600min. The initial sludge is expanded, the sludge settling performance is 200mL/g, the sludge mass concentration (MLSS) is about 1.5g/L, and the activated sludge mass concentration (MLVSS) is about 0.75g/L. 15d is operated, the SVI reaches 225mL/g, the biological enzyme compound, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride are weighed according to the weight part ratio described in the example 3, the mixture is added into the sludge, the adding amount of the biological enzyme preparation is 20mg/L of the sludge, 25d is reached, and the SVI is stabilized at 130mL/g. Illustration the bio-enzyme formulation of example 3 was added to effectively control sludge bulking.
TABLE 5 sludge bulking process control parameters
| Run time (d) | MLSS(g/L) | DO(mg/L) | SVI(mL/g) |
| 0～10 | 4.89±0.37 | 0.25～0.33 | 200.75±20.93 |
| 11～15 | 4.67±0.46 | 0.33 | 225.13±18.00 |
| 25 | 3.88±0.84 | 0.30～0.34 | 130.00±20.30 |
Finally, it should be noted that the above only illustrates the technical solution of the present invention and is not limiting, and although the present invention has been described in detail with reference to the preferred arrangement, it should be understood by those skilled in the art that modifications and equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention.
Claims (6)
1. A filamentous fungus expanded sludge control biological enzyme preparation, characterized in that the biological enzyme preparation comprises a biological enzyme complex, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride, wherein the biological enzyme complex is a mixture of cellulase, hemicellulase, amylase and protease;
the weight portions of the raw materials are as follows:
cellulase 10
Hemicellulase 5
Amylase 5
Protease 10
Silica 30
Potassium phosphate 10
Ammonium nitrate 20
Magnesium chloride 10;
or (b)
Cellulase 10
Hemicellulase 5
Amylase 5
Protease 15
Silica 25
Potassium phosphate 10
Ammonium nitrate 20
Magnesium chloride 10;
or (b)
Cellulase 10
Hemicellulase 10
Amylase 10
Protease 10
Silica 20
Potassium phosphate 10
Ammonium nitrate 20
Magnesium chloride 10.
2. The filamentous fungus expanded sludge control biological enzyme preparation according to claim 1, wherein the cellulases and hemicellulases are of fungal, bacterial or plant origin.
3. A method of preparing a filamentous fungus expanded sludge control biological enzyme preparation as claimed in claim 1 or 2, comprising the steps of:
step 1, dissolving: dissolving the biological enzyme complex in a solvent, wherein the solvent is one of water, citric acid buffer solution, phosphoric acid buffer solution, tris (Tris) hydrochloric acid solution, polyethylene glycol and polyvinylpyrrolidone in different proportions to obtain an enzyme preparation mixed solution;
step 2, freeze-drying: adding a freeze-drying protective agent into the enzyme preparation mixed solution, and performing freeze-drying treatment to obtain freeze-drying enzyme preparation mixed powder;
and 3, respectively weighing the mixed powder of the freeze-dried enzyme preparation, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride according to the weight part ratio of the filamentous fungus expanded sludge control biological enzyme preparation, and independently packaging and storing the mixed powder, the silicon dioxide, the potassium phosphate, the ammonium nitrate and the magnesium chloride to obtain the filamentous fungus expanded sludge control biological enzyme preparation.
4. The method for preparing a filamentous fungus expanded sludge controlled biological enzyme preparation according to claim 3, wherein in the step 2, the addition amount of the freeze-drying protective agent is 1% -10% of the mass of the enzyme preparation mixed solution, and the freeze-drying protective agent is one of sucrose, starch, trehalose, lactose, maltose, PEG2000, PEG6000, sodium dihydrogen phosphate, sodium glutamate and sorbitol.
5. The method for producing a filamentous fungus expanded sludge control biological enzyme preparation according to claim 3, wherein in the step 2, the condition of the lyophilization treatment is: vacuum degree is 0-10 Pa, temperature is-30 ℃ to-50 ℃, and freeze-drying time is 24-72 h.
6. A method of treatment for filamentous fungus expanded sludge control using the biological enzyme preparation according to claim 1 or 2, characterized in that the method comprises the steps of:
weighing the biological enzyme complex, silicon dioxide, potassium phosphate, ammonium nitrate and magnesium chloride according to the weight part ratio of claim 1; and adding the obtained biological enzyme preparation into sludge to be treated according to the addition amount of 10 mg/L-30 mg/L.
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