CN111500291B - Enzyme-catalyzed conditioner for restoring soil and preparation method and application thereof - Google Patents
Enzyme-catalyzed conditioner for restoring soil and preparation method and application thereof Download PDFInfo
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- CN111500291B CN111500291B CN202010322001.3A CN202010322001A CN111500291B CN 111500291 B CN111500291 B CN 111500291B CN 202010322001 A CN202010322001 A CN 202010322001A CN 111500291 B CN111500291 B CN 111500291B
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- 239000002689 soil Substances 0.000 title claims abstract description 98
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- SOQBVABWOPYFQZ-UHFFFAOYSA-N oxygen(2-);titanium(4+) Chemical compound [O-2].[O-2].[Ti+4] SOQBVABWOPYFQZ-UHFFFAOYSA-N 0.000 claims abstract description 51
- 239000003337 fertilizer Substances 0.000 claims abstract description 45
- 108090000790 Enzymes Proteins 0.000 claims abstract description 37
- 102000004190 Enzymes Human genes 0.000 claims abstract description 37
- 229940088598 enzyme Drugs 0.000 claims abstract description 37
- 238000006555 catalytic reaction Methods 0.000 claims abstract description 36
- 150000002894 organic compounds Chemical class 0.000 claims abstract description 36
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000004021 humic acid Substances 0.000 claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 27
- 239000010902 straw Substances 0.000 claims abstract description 27
- 108010059892 Cellulase Proteins 0.000 claims abstract description 25
- 229940106157 cellulase Drugs 0.000 claims abstract description 25
- 239000004215 Carbon black (E152) Substances 0.000 claims abstract description 19
- 229930195733 hydrocarbon Natural products 0.000 claims abstract description 19
- 150000002430 hydrocarbons Chemical class 0.000 claims abstract description 19
- 238000005067 remediation Methods 0.000 claims abstract description 19
- 239000010802 sludge Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical group O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 238000005303 weighing Methods 0.000 claims description 11
- 239000003607 modifier Substances 0.000 claims description 10
- 229920006395 saturated elastomer Polymers 0.000 claims description 8
- 239000012535 impurity Substances 0.000 claims description 5
- 239000001913 cellulose Substances 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 230000003750 conditioning effect Effects 0.000 claims 4
- 230000008439 repair process Effects 0.000 abstract description 16
- 238000000034 method Methods 0.000 abstract description 15
- 239000005416 organic matter Substances 0.000 abstract description 10
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000003197 catalytic effect Effects 0.000 abstract 1
- 230000008263 repair mechanism Effects 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 18
- 239000003209 petroleum derivative Substances 0.000 description 16
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 9
- 238000012423 maintenance Methods 0.000 description 9
- 229910052757 nitrogen Inorganic materials 0.000 description 9
- 229910052698 phosphorus Inorganic materials 0.000 description 9
- 239000011574 phosphorus Substances 0.000 description 9
- 150000003112 potassium compounds Chemical class 0.000 description 9
- 239000000126 substance Substances 0.000 description 8
- 238000005496 tempering Methods 0.000 description 7
- 239000003344 environmental pollutant Substances 0.000 description 6
- 231100000719 pollutant Toxicity 0.000 description 6
- 239000003208 petroleum Substances 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000010276 construction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 238000001139 pH measurement Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000011895 specific detection Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000013043 chemical agent Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000010779 crude oil Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011066 ex-situ storage Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000003802 soil pollutant Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Inorganic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials Engineering (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention is applicable to the technical field of soil remediation, and provides an enzyme catalysis conditioner for soil remediation, a preparation method and application thereof, wherein the enzyme catalysis conditioner comprises the following components in percentage by mass: 0.2 to 2 percent of nano titanium dioxide, 2 to 20 percent of humic acid, 15 to 40 percent of organic compound fertilizer, 40 to 80 percent of straw powder and 1 to 5 percent of cellulase. The enzyme catalysis conditioner can be used for repairing hydrocarbon organic matter polluted soil. According to the invention, the repair mechanism of the soil polluted by hydrocarbon organic matters is revolutionarily changed through the nano titanium dioxide and enzyme catalytic repair technology, the repair time of the soil polluted by hydrocarbon organic matters is quickened, the basic properties of the soil are quickly recovered, the method is simple and convenient to implement, the cost is low, secondary pollution is avoided, and the method accords with the national environmental protection industry policy and development direction.
Description
Technical Field
The invention belongs to the technical field of soil remediation, and particularly relates to an enzyme catalysis conditioner for soil remediation, a preparation method and application thereof.
Background
With the global industrialization and countryside urbanization progress of China, the hydrocarbon organic matter (petroleum) pollution is advanced over other pollution such as heavy metal, the most serious hydrocarbon organic matter pollution in the soil is petroleum pollution, the oil content of the shallow soil and the underground water on the surface of most oil areas is seriously higher, the safety and the ecological environment of surrounding people are seriously endangered, and the repair work is urgent.
In the face of soil pollution, although several tens of physicochemical and biological methods have been developed in the prior art for treating organic-polluted soil, each treatment method has limitations due to the diversity of organic pollutants and the nature of soil itself. Hydrocarbon organic contamination of soil (e.g., petroleum) has long been one of the focus of existing soil remediation.
The main methods of the current hydrocarbon organic matter polluted soil restoration are physical restoration, chemical restoration and biological restoration, and the soil physical restoration is a method for separating by physical means based on the physical and chemical properties of the soil and the different characteristics of organic pollutants; the soil chemical restoration is to utilize oxidant and reducer to carry out chemical treatment on the polluted soil by means of in-situ injection or ex-situ addition, etc., so that the pollutants in the soil are converted into non-toxic or relatively less toxic substances. Soil bioremediation refers to the reduction of soil pollutant content or the conversion of toxic and harmful substances into harmless substances by the absorption, degradation and conversion of pollutants in soil by soil organisms (including plants, animals and microorganisms acting alone or in combination).
However, physical repair, chemical repair or biological repair have many factors that affect the actual treatment effect, and also cause different hazards. Physical repair is often high in energy consumption, complicated and huge in equipment, high in investment and often not up to the standard in separation effect; in the chemical restoration process, different chemical agents are required to be added into the soil, and the rapid change of the pH value and heat release of the soil are accompanied in the chemical agent addition process, so that the soil is highly salinized, the organic matters are lost, desertification is formed in the restored soil, and the soil is not grown; the bioremediation is more affected factors, the property, permeability, pH value, pollutant type, state and environment of the soil are changed, the bioremediation effect is affected, the final restoration is greatly reduced, the restoration period is prolonged, and even repeated restoration is needed for many times, so that secondary pollution is caused to organic matter enrichment.
Therefore, a method for restoring hydrocarbon organic matter polluted soil, which can reduce secondary pollution of soil and return original properties of soil and has a short restoration period, is needed to be sought.
Disclosure of Invention
The embodiment of the invention aims to provide an enzyme catalysis conditioner for soil remediation, and aims to solve the problems in the background technology.
The embodiment of the invention is realized in such a way that the enzyme catalysis conditioner for soil remediation comprises the following components in percentage by mass: 0.2 to 2 percent of nano titanium dioxide, 2 to 20 percent of humic acid, 15 to 40 percent of organic compound fertilizer, 40 to 80 percent of straw powder and 1 to 5 percent of cellulase, wherein the sum of mass fractions of the components is 100 percent.
As a preferred scheme of the embodiment of the invention, the enzyme catalysis modifier comprises the following components in percentage by mass: 0.5 to 1 percent of nano titanium dioxide, 5 to 15 percent of humic acid, 20 to 30 percent of organic compound fertilizer, 50 to 72.5 percent of straw powder and 2 to 4 percent of cellulose, wherein the sum of the mass fractions of the components is 100 percent.
As another preferable aspect of the embodiment of the invention, the nano titanium dioxide is anatase nano titanium dioxide.
Another object of the embodiment of the present invention is to provide a preparation method of the above enzyme-catalyzed tempering agent, which includes the following steps:
weighing nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase according to the mass fractions of the components for standby;
mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme-catalyzed modifier.
Another object of the embodiment of the invention is to provide an enzyme-catalyzed tempering agent prepared by the preparation method.
Another object of an embodiment of the present invention is to provide an application of the above enzyme-catalyzed tempering agent in repairing soil and/or sludge contaminated with hydrocarbon organics.
As another preferable mode of the embodiment of the invention, the method comprises the following steps:
removing impurities from the soil and/or oil sludge to be restored, and then crushing and homogenizing to obtain a sample to be restored;
controlling the pH value of the sample to be repaired to be 5-9, and then mixing the sample with the enzyme catalysis modifier to obtain the sample to be maintained;
and adding water into the sample to be maintained to ensure that the saturated water holding capacity is not less than 60%, and maintaining to finish the restoration treatment of the soil and/or the oil sludge to be restored.
As another preferable scheme of the embodiment of the invention, in the step, the mass of the enzyme catalysis modifier is 0.5-2.5% of the mass of the sample to be repaired.
As another preferable mode of the embodiment of the invention, in the step, the curing temperature is not lower than 15 ℃.
As another preferable scheme of the embodiment of the invention, in the step, the curing time is 4-10 weeks.
According to the enzyme catalysis conditioner for repairing soil, provided by the embodiment of the invention, the repairing mechanism of the soil polluted by hydrocarbon organic matters is revolutionarily changed through the nano titanium dioxide and enzyme catalysis repairing technology, the repairing time of the soil polluted by hydrocarbon organic matters is shortened, the basic properties of the soil are quickly recovered, the enzyme catalysis conditioner is simple and convenient to implement, the cost is low, secondary pollution is avoided, and the environment-friendly industrial policy and development direction of China are met.
Drawings
FIG. 1 is a flow chart of a method for soil remediation by using the enzyme-catalyzed conditioner provided by the embodiment of the invention.
FIG. 2 is a graph showing the variation of total petroleum hydrocarbon amounts of the sample to be cured at various curing times in example 10 of the present invention.
FIG. 3 is a graph showing the variation of total petroleum hydrocarbon amounts of the sample to be cured at various curing times in example 11 of the present invention.
FIG. 4 is a graph showing the variation of total petroleum hydrocarbon amounts of the sample to be cured at various curing times in example 12 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Example 1
The embodiment provides an enzyme catalysis conditioner for soil remediation, which comprises the following steps:
s1, weighing 0.2kg of nano titanium dioxide, 2kg of humic acid, 15kg of organic compound fertilizer, 80kg of straw powder and 2.8kg of cellulase for standby; wherein the nano titanium dioxide is anatase type nano titanium dioxide; the organic compound fertilizer can be a common nitrogen, phosphorus and potassium compound fertilizer sold in the prior market, and can be specifically selected according to the soil quality of the soil to be repaired according to actual needs.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 2
The embodiment provides an enzyme catalysis conditioner for soil remediation, which comprises the following steps:
s1, weighing 2kg of nano titanium dioxide, 20kg of humic acid, 33kg of organic compound fertilizer, 40kg of straw powder and 5kg of cellulase for later use; wherein the nano titanium dioxide is anatase type nano titanium dioxide; the organic compound fertilizer can be a common nitrogen, phosphorus and potassium compound fertilizer sold in the prior market, and can be specifically selected according to the soil quality of the soil to be repaired according to actual needs.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 3
The embodiment provides an enzyme catalysis conditioner for soil remediation, which comprises the following steps:
s1, weighing 1kg of nano titanium dioxide, 8kg of humic acid, 40kg of organic compound fertilizer, 50kg of straw powder and 1kg of cellulase for later use; wherein the nano titanium dioxide is anatase type nano titanium dioxide; the organic compound fertilizer can be a common nitrogen, phosphorus and potassium compound fertilizer sold in the prior market, and can be specifically selected according to the soil quality of the soil to be repaired according to actual needs.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 4
The embodiment provides an enzyme catalysis conditioner for soil remediation, which comprises the following steps:
s1, weighing 0.5kg of nano titanium dioxide, 5kg of humic acid, 20kg of organic compound fertilizer, 72.5kg of straw powder and 2kg of cellulase for standby; wherein the nano titanium dioxide is anatase type nano titanium dioxide; the organic compound fertilizer can be a common nitrogen, phosphorus and potassium compound fertilizer sold in the prior market, and can be specifically selected according to the soil quality of the soil to be repaired according to actual needs.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 5
The embodiment provides an enzyme catalysis conditioner for soil remediation, which comprises the following steps:
s1, weighing 1kg of nano titanium dioxide, 15kg of humic acid, 30kg of organic compound fertilizer, 50kg of straw powder and 4kg of cellulase for later use; wherein the nano titanium dioxide is anatase type nano titanium dioxide; the organic compound fertilizer can be a common nitrogen, phosphorus and potassium compound fertilizer sold in the prior market, and can be specifically selected according to the soil quality of the soil to be repaired according to actual needs.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 6
The embodiment provides an enzyme catalysis conditioner for soil remediation, which comprises the following steps:
s1, weighing 0.8kg of nano titanium dioxide, 10kg of humic acid, 25kg of organic compound fertilizer, 61.2kg of straw powder and 3kg of cellulase for standby; wherein the nano titanium dioxide is anatase type nano titanium dioxide; the organic compound fertilizer can be a common nitrogen, phosphorus and potassium compound fertilizer sold in the prior market, and can be specifically selected according to the soil quality of the soil to be repaired according to actual needs.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 7
The embodiment provides an enzyme catalysis conditioner for soil remediation, which comprises the following steps:
s1, weighing 1kg of nano titanium dioxide, 8kg of humic acid, 30kg of organic compound fertilizer, 58.5kg of straw powder and 2.5kg of cellulase for standby; wherein the nano titanium dioxide is anatase type nano titanium dioxide; the organic compound fertilizer can be a common nitrogen, phosphorus and potassium compound fertilizer sold in the prior market, and can be specifically selected according to the soil quality of the soil to be repaired according to actual needs.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 8
The embodiment provides an enzyme catalysis conditioner for soil remediation, which comprises the following steps:
s1, weighing 0.5kg of nano titanium dioxide, 10kg of humic acid, 25kg of organic compound fertilizer, 61kg of straw powder and 3.5kg of cellulase for standby; wherein the nano titanium dioxide is anatase type nano titanium dioxide; the organic compound fertilizer can be a common nitrogen, phosphorus and potassium compound fertilizer sold in the prior market, and can be specifically selected according to the soil quality of the soil to be repaired according to actual needs.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 9
The embodiment provides an enzyme catalysis conditioner for soil remediation, which comprises the following steps:
s1, weighing 1.8kg of nano titanium dioxide, 16kg of humic acid, 35kg of organic compound fertilizer, 46kg of straw powder and 1.2kg of cellulase for standby; wherein the nano titanium dioxide is anatase type nano titanium dioxide; the organic compound fertilizer can be a common nitrogen, phosphorus and potassium compound fertilizer sold in the prior market, and can be specifically selected according to the soil quality of the soil to be repaired according to actual needs.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 10
The embodiment provides the application of the enzyme-catalyzed tempering agent provided in the embodiment 7 in the remediation of certain hydrocarbon organic pollution sites in the northeast. Specifically, the hydrocarbon organic matter pollution site is located in a certain northeast area, the polluted soil contains a large amount of oil sludge mixture discharged from the petroleum exploitation process, the oil sludge mixture is high in pollution concentration, the oil sludge is in a black viscous state, the restoration target is to reduce the total content of petroleum hydrocarbon contained in the oil sludge polluted soil to below 2% of comprehensive utilization pollution control index (20000 mg/kg) of oil field oil-containing sludge, and the restoration method is shown in an attached figure 1 and specifically comprises the following steps:
s1, firstly taking contaminated soil, carrying out impurity removal treatment to remove stones and plant residues, and then crushing and homogenizing by using a mixer to obtain a sample to be repaired.
S2, carrying out pH measurement on the sample to be repaired, wherein the test result is 7.98, and the pH value does not need to be regulated; then, 200 kg of a sample to be repaired is taken, 4kg of the enzyme catalysis modifier provided in the embodiment 7 is weighed according to the pollutant components, equivalent and other data detected by the original sample of the polluted soil, and is added into the sample to be repaired, and the sample to be maintained is obtained after uniform mixing.
S3, preparing the sample to be maintained into a biological pile, wherein the pile size is about 1 x 0.8 x 0.3 (length is greater than width is higher than width is unit is meter), spraying 20kg of water (moderately adjusting according to the water content of the polluted soil), so that the saturated water holding capacity of the soil in the sample to be maintained reaches a saturated state of more than 80%, and maintaining; wherein the maintenance environment temperature is more than 15 ℃, and the maintenance environment temperature is twice turned and planed every day; the curing time was continued for 10 weeks.
And (3) sampling and detecting the sample to be cured, which is cured, wherein specific detection data are shown in table 1 and figure 2.
TABLE 1
As can be seen from the table 1 and the attached figure 2, after curing for 6 weeks, the total content of petroleum hydrocarbon in the polluted soil is reduced to below 20000mg/kg, and the oil field industry repair standard is reached; after maintenance for 8 weeks, the total content of petroleum hydrocarbon is reduced to less than 3000mg/kg, and the restoration standard of the agricultural industry is reached; after maintenance for 10 weeks, the total content of petroleum hydrocarbon is reduced to less than 826mg/kg, and the standard of repairing the screening value of the national construction land is reached.
Example 11
The embodiment provides the application of the enzyme-catalyzed tempering agent provided in the embodiment 8 in the restoration of a hydrocarbon organic polluted site in North China. Specifically, the pollution site is located in a certain area of North China, and crude oil generates certain leakage and overflow due to long-term aging of an oil pipeline, so that peripheral soil is polluted for a certain period. The restoration target is to reduce the total content of petroleum hydrocarbon contained in the soil polluted by the oil sludge from 38200mg/kg to less than 3000mg/kg of pollution control index of agricultural industry for comprehensively utilizing the oil sludge in the oil field, and the restoration method is shown in the attached figure 1, and specifically comprises the following steps:
s1, firstly taking contaminated soil, carrying out impurity removal treatment to remove stones and plant residues, and then crushing and homogenizing by using a mixer to obtain a sample to be repaired.
S2, carrying out pH measurement on the sample to be repaired, wherein the test result is 8.3, and the pH value does not need to be regulated; then, 300 kg of a sample to be repaired is taken, 4.5 kg of the enzyme catalysis modifier provided in the embodiment 8 is weighed according to the data of pollutant components, equivalent and the like detected by the original sample of the polluted soil, and is added into the sample to be repaired, and the sample to be maintained is obtained by uniformly mixing.
S3, preparing the sample to be maintained into a biological pile, wherein the pile size is about 1 x 0.8 x 0.4 (length is greater than width is higher than width is unit is meter), spraying 30kg of water (moderately adjusted according to the water content of the polluted soil), so that the saturated water holding capacity of the soil in the sample to be maintained reaches a saturated state of more than 80%, and maintaining; wherein the maintenance environment temperature is more than 15 ℃, and the maintenance environment temperature is twice turned and planed every day; the curing time was continued for 10 weeks.
And (3) sampling and detecting the sample to be cured, which is cured, wherein specific detection data are shown in table 2 and figure 3.
TABLE 2
As can be seen from the table 2 and the attached figure 3, after curing for 4 weeks, the total content of petroleum hydrocarbon is reduced to below 20000mg/kg, and the repairing standard of the oil field industry is reached; after curing for 7 weeks, the total content of petroleum hydrocarbon is reduced to less than 3000mg/kg, and the repairing standard of the agricultural industry is reached; after curing for 9 weeks, the total content of petroleum hydrocarbon is reduced to less than 826mg/kg, and the method reaches the national land screening value restoration standard.
Example 12
The embodiment provides the application of the enzyme-catalyzed tempering agent provided in the embodiment 9 in the repair of a hydrocarbon organic matter polluted site in the southwest. Specifically, the contaminated site is located in a region in the southwest, the contaminated soil contains a large amount of oil sludge falling to the ground, the total petroleum hydrocarbon content of the contaminated soil is 93486mg/kg, the repairing target is to reduce the total petroleum hydrocarbon content of the contaminated soil to below 2% of the comprehensive utilization pollution control index (20000 mg/kg) of the oil-containing sludge of the oil field, and the repairing method is shown in the attached figure 1 and specifically comprises the following steps:
s1, firstly taking contaminated soil, carrying out impurity removal treatment to remove stones and plant residues, and then crushing and homogenizing by using a mixer to obtain a sample to be repaired.
S2, carrying out pH measurement on the sample to be repaired, wherein the test result is 8.98, and the pH value does not need to be regulated; then, 200 kg of sample to be repaired is taken, 5kg of the enzyme catalysis modifier provided in the embodiment 9 is weighed according to the data of pollutant components, equivalent and the like detected by the original sample of the polluted soil, and is added into the sample to be repaired, and the sample to be maintained is obtained after uniform mixing.
S3, preparing the sample to be cured into a biological pile, wherein the pile size is about 1 x 0.6 x 0.3 (length is greater than width is higher than width is unit is meter), spraying 18 kg of water (moderately adjusting according to the water content of the polluted soil), so that the saturated water holding capacity of the soil in the sample to be cured reaches a saturated state of more than 60%, and curing; wherein the maintenance environment temperature is more than 15 ℃, and the maintenance environment temperature is twice turned and planed every day; the curing time was continued for 10 weeks.
And (3) sampling and detecting the sample to be cured, which is cured, wherein specific detection data are shown in table 3 and fig. 4.
TABLE 3 Table 3
As can be seen from Table 3 and figure 4, after curing for 7 weeks, the total content of petroleum hydrocarbon has been reduced to below 20000mg/kg, which reaches the oil field industry repair standard; after curing for 9 weeks, the total content of petroleum hydrocarbon is reduced to less than 3000mg/kg, and the repairing standard of the agricultural industry is reached; after maintenance for 10 weeks, the total content of petroleum hydrocarbon is reduced to less than 826mg/kg, and the standard of repairing the screening value of the national construction land is reached.
In conclusion, the enzyme-catalyzed tempering agent provided by the embodiment of the invention can rapidly repair complex hydrocarbon organic matter (petroleum) polluted soil, provides a low-cost green repair material which is simple and reliable in construction, free of secondary environmental pollution, short in repair treatment period and free of microorganism culture, not only effectively shortens the repair time of hydrocarbon organic matter polluted soil, but also saves a large amount of funds for repair treatment units and owners, and meanwhile, secondary pollution is not generated in the repair treatment areas, the purpose of rapidly repairing and recovering the original properties of soil is truly achieved, and the environmental benefit, economic benefit and social benefit are very remarkable.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (2)
1. Use of an enzyme-catalyzed conditioning agent for repairing soil and/or sludge contaminated with hydrocarbon organics, characterized in that the enzyme-catalyzed conditioning agent comprises the following components in mass fraction: 0.2 to 2 percent of nano titanium dioxide, 2 to 20 percent of humic acid, 15 to 40 percent of organic compound fertilizer, 40 to 80 percent of straw powder and 1 to 5 percent of cellulase, wherein the sum of the mass fractions of the components is 100 percent;
the application comprises the following steps:
removing impurities from the soil and/or oil sludge to be restored, and then crushing and homogenizing to obtain a sample to be restored;
controlling the pH value of the sample to be repaired to be 5-9, and then mixing the sample with the enzyme catalysis modifier to obtain the sample to be maintained;
adding water into the sample to be maintained to ensure that the saturated water holding capacity is not less than 60%, and maintaining to finish the restoration treatment of the soil and/or the oil sludge to be restored;
wherein, in the step, the mass of the enzyme catalysis modifier is 0.5-2.5% of the mass of the sample to be repaired; maintaining at a temperature not lower than 15 ℃; the curing time is 4-10 weeks; the nano titanium dioxide is anatase type nano titanium dioxide; the preparation method of the enzyme catalysis conditioning agent comprises the following steps:
weighing nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase according to the mass fractions of the components for standby;
mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme-catalyzed modifier.
2. Use of an enzyme-catalyzed conditioning agent according to claim 1 for the remediation of soils and/or sludge contaminated with hydrocarbon organics, characterized in that it comprises the following components in mass fraction: 0.5 to 1 percent of nano titanium dioxide, 5 to 15 percent of humic acid, 20 to 30 percent of organic compound fertilizer, 50 to 72.5 percent of straw powder and 2 to 4 percent of cellulose, wherein the sum of the mass fractions of the components is 100 percent.
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