CN111500291A - Enzyme catalysis conditioning agent for soil remediation and preparation method and application thereof - Google Patents
Enzyme catalysis conditioning agent for soil remediation and preparation method and application thereof Download PDFInfo
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- CN111500291A CN111500291A CN202010322001.3A CN202010322001A CN111500291A CN 111500291 A CN111500291 A CN 111500291A CN 202010322001 A CN202010322001 A CN 202010322001A CN 111500291 A CN111500291 A CN 111500291A
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- 239000002689 soil Substances 0.000 title claims abstract description 91
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 37
- 238000005067 remediation Methods 0.000 title claims abstract description 35
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 34
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 34
- 238000006555 catalytic reaction Methods 0.000 title claims abstract description 34
- 230000003750 conditioning effect Effects 0.000 title claims description 23
- 238000002360 preparation method Methods 0.000 title abstract description 14
- SOQBVABWOPYFQZ-UHFFFAOYSA-N oxygen(2-);titanium(4+) Chemical compound [O-2].[O-2].[Ti+4] SOQBVABWOPYFQZ-UHFFFAOYSA-N 0.000 claims abstract description 49
- 239000003337 fertilizer Substances 0.000 claims abstract description 45
- 150000002894 organic compounds Chemical class 0.000 claims abstract description 36
- 229940088598 enzyme Drugs 0.000 claims abstract description 33
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 27
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- 229930195733 hydrocarbon Natural products 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 16
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical group O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
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- 230000008439 repair process Effects 0.000 description 11
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- 229910052757 nitrogen Inorganic materials 0.000 description 9
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The invention is suitable for the technical field of soil remediation and provides an enzyme catalysis conditioner for soil remediation and a preparation method and application thereof, wherein the enzyme catalysis conditioner comprises the following components in percentage by mass: 0.2-2% of nano titanium dioxide, 2-20% of humic acid, 15-40% of organic compound fertilizer, 40-80% of straw powder and 1-5% of cellulase. The enzyme catalysis modifying agent can be used for repairing the soil polluted by hydrocarbon organic matters. The method revolutionarily changes the remediation mechanism of the soil polluted by the hydrocarbon organic matters through the nano titanium dioxide and enzyme catalysis remediation technology, accelerates the remediation time of the soil polluted by the hydrocarbon organic matters, quickly recovers the basic properties of the soil, is simple and convenient to implement, has low cost, avoids secondary pollution, and accords with the national environmental protection industry policy and development direction.
Description
Technical Field
The invention belongs to the technical field of soil remediation, and particularly relates to an enzyme catalysis conditioner for soil remediation, and a preparation method and application thereof.
Background
As a limited natural resource on which human beings rely to live, with the progress of global industrialization and rural urbanization in China, the pollution of hydrocarbon organic matters (petroleum) exceeds other pollutions such as heavy metals and the like to jump the top, the most serious pollution of the hydrocarbon organic matters in the soil is petroleum pollution, the oil content of shallow soil and underground water on the earth surface of most oil areas is seriously high, the environmental safety and ecological environment are seriously damaged, and the repair work is urgent.
In the face of soil pollution, although several dozen physicochemical and biological methods have been developed in the prior art for remediating organic-contaminated soil, each remediation method has limitations due to the diversity of the organic pollutants and the nature of the soil itself. Hydrocarbon contamination of soils (e.g., petroleum) has long been one of the major concerns in existing soil remediation.
The current main methods for repairing the soil polluted by hydrocarbon organic matters are physical repair, chemical repair and biological repair, wherein the physical repair of the soil is a method for separating the soil by physical means based on the physical and chemical properties of the soil and different characteristics of organic pollutants; the chemical remediation of soil is to utilize oxidant and reductant to chemically treat polluted soil in-situ injection or ectopic addition mode to convert the pollutant in soil into non-toxic or relatively less toxic matter. Soil bioremediation refers to absorption, degradation and transformation of pollutants in soil by soil organisms (including plants, animals and microorganisms acting alone or in combination) to reduce the content of soil pollutants or to transform toxic and harmful substances into harmless substances.
However, whether physical, chemical or biological, their actual treatment is affected by a number of factors and can also be harmful. Physical remediation is often high in energy consumption, complex and large in used equipment, high in investment and often unqualified in separation effect; different chemical agents are required to be added into the soil in the chemical remediation process, the chemical agent addition process is accompanied by the rapid change and heat release of the pH value of the soil, the soil is highly salinized, organic matters are lost, the remediated soil can form desertification, and the weeds cannot grow; the bioremediation is influenced by more factors, and the properties, permeability, pH value, pollutant types, states and environment of soil are changed, so that the bioremediation effect is influenced, the final remediation is greatly reduced, the remediation period is prolonged, and even repeated remediation is needed for many times, so that secondary pollution caused by organic matter enrichment is caused.
Therefore, a method for restoring soil polluted by hydrocarbon organic matters, which can reduce secondary pollution of soil, return original properties of soil and has a short restoration period, is urgently needed.
Disclosure of Invention
An object of an embodiment of the present invention is to provide an enzyme-catalyzed conditioner for soil remediation, which is directed to solving the problems set forth in the background art.
The embodiment of the invention is realized in such a way that the enzyme-catalyzed conditioning agent for repairing soil comprises the following components in percentage by mass: 0.2-2% of nano titanium dioxide, 2-20% of humic acid, 15-40% of organic compound fertilizer, 40-80% of straw powder and 1-5% of cellulase, wherein the sum of the mass fractions of the components is 100%.
As a preferable scheme of the embodiment of the invention, the enzyme catalysis modifying agent comprises the following components in percentage by mass: 0.5-1% of nano titanium dioxide, 5-15% of humic acid, 20-30% of organic compound fertilizer, 50-72.5% of straw powder and 2-4% of cellulase, wherein the sum of the mass fractions of the components is 100%.
As another preferable scheme of the embodiment of the present invention, the nano titanium dioxide is anatase-type nano titanium dioxide.
Another objective of the embodiments of the present invention is to provide a method for preparing the enzyme catalysis modifying agent, which comprises the following steps:
weighing nanometer titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase according to the mass fractions of the components for later use;
and mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioning agent.
The embodiment of the invention also aims to provide the enzyme catalysis modifying agent prepared by the preparation method.
Another object of an embodiment of the present invention is to provide an application of the above enzyme-catalyzed modifying agent in repairing soil and/or oil sludge contaminated by hydrocarbon organic matters.
As another preferable scheme of the embodiment of the invention, the method comprises the following steps:
after the soil and/or oil sludge to be repaired is subjected to impurity removal treatment, crushing treatment and homogenization treatment are carried out to obtain a sample to be repaired;
controlling the pH value of a sample to be repaired to be 5-9, and then mixing the sample with the enzyme catalysis modifying agent to obtain a sample to be maintained;
and adding water into the sample to be maintained to enable the saturated water holding capacity of the sample to be maintained to be not less than 60%, and maintaining to finish the repairing treatment of the soil and/or the oil sludge to be repaired.
As another preferable scheme of the embodiment of the invention, in the step, the mass of the enzyme catalysis tempering agent is 0.5-2.5% of the mass of the sample to be repaired.
As another preferable scheme of the embodiment of the invention, in the step, the curing temperature is not lower than 15 ℃.
In another preferable embodiment of the present invention, in the step, the curing time is 4 to 10 weeks.
The enzyme catalysis conditioner for repairing the soil provided by the embodiment of the invention revolutionarily changes the repairing mechanism of the soil polluted by hydrocarbon organic matters through the nano titanium dioxide and enzyme catalysis repairing technology, accelerates the repairing time of the soil polluted by the hydrocarbon organic matters, quickly recovers the basic attribute of the soil, is simple and convenient to implement, has low cost, avoids secondary pollution, and accords with the national environmental protection industry policy and development direction.
Drawings
FIG. 1 is a flow chart of a method for soil remediation by using an enzyme-catalyzed conditioning agent provided by an embodiment of the invention.
FIG. 2 is a graph showing the change in the total petroleum hydrocarbon amount of the samples to be cured at different curing times in example 10 of the present invention.
FIG. 3 is a graph showing the change in the total petroleum hydrocarbon amount of the samples to be cured at different curing times in example 11 of the present invention.
FIG. 4 is a graph showing the change in the total petroleum hydrocarbon amount of the samples to be cured at different curing times in example 12 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
The embodiment provides an enzyme-catalyzed conditioning agent for soil remediation, and the preparation method comprises the following steps:
s1, weighing 0.2kg of nano titanium dioxide, 2kg of humic acid, 15kg of organic compound fertilizer, 80kg of straw powder and 2.8kg of cellulase for later use; wherein, the nano titanium dioxide is anatase nano titanium dioxide; the organic compound fertilizer can adopt the existing common nitrogen, phosphorus and potassium compound fertilizers sold in the market, and can be selected according to the actual need for soil restoration.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 2
The embodiment provides an enzyme-catalyzed conditioning agent for soil remediation, and the preparation method comprises the following steps:
s1, weighing 2kg of nano titanium dioxide, 20kg of humic acid, 33kg of organic compound fertilizer, 40kg of straw powder and 5kg of cellulase for later use; wherein, the nano titanium dioxide is anatase nano titanium dioxide; the organic compound fertilizer can adopt the existing common nitrogen, phosphorus and potassium compound fertilizers sold in the market, and can be selected according to the actual need for soil restoration.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 3
The embodiment provides an enzyme-catalyzed conditioning agent for soil remediation, and the preparation method comprises the following steps:
s1, weighing 1kg of nano titanium dioxide, 8kg of humic acid, 40kg of organic compound fertilizer, 50kg of straw powder and 1kg of cellulase for later use; wherein, the nano titanium dioxide is anatase nano titanium dioxide; the organic compound fertilizer can adopt the existing common nitrogen, phosphorus and potassium compound fertilizers sold in the market, and can be selected according to the actual need for soil restoration.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 4
The embodiment provides an enzyme-catalyzed conditioning agent for soil remediation, and the preparation method comprises the following steps:
s1, weighing 0.5kg of nano titanium dioxide, 5kg of humic acid, 20kg of organic compound fertilizer, 72.5kg of straw powder and 2kg of cellulase for later use; wherein, the nano titanium dioxide is anatase nano titanium dioxide; the organic compound fertilizer can adopt the existing common nitrogen, phosphorus and potassium compound fertilizers sold in the market, and can be selected according to the actual need for soil restoration.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 5
The embodiment provides an enzyme-catalyzed conditioning agent for soil remediation, and the preparation method comprises the following steps:
s1, weighing 1kg of nano titanium dioxide, 15kg of humic acid, 30kg of organic compound fertilizer, 50kg of straw powder and 4kg of cellulase for later use; wherein, the nano titanium dioxide is anatase nano titanium dioxide; the organic compound fertilizer can adopt the existing common nitrogen, phosphorus and potassium compound fertilizers sold in the market, and can be selected according to the actual need for soil restoration.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 6
The embodiment provides an enzyme-catalyzed conditioning agent for soil remediation, and the preparation method comprises the following steps:
s1, weighing 0.8kg of nano titanium dioxide, 10kg of humic acid, 25kg of organic compound fertilizer, 61.2kg of straw powder and 3kg of cellulase for later use; wherein, the nano titanium dioxide is anatase nano titanium dioxide; the organic compound fertilizer can adopt the existing common nitrogen, phosphorus and potassium compound fertilizers sold in the market, and can be selected according to the actual need for soil restoration.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 7
The embodiment provides an enzyme-catalyzed conditioning agent for soil remediation, and the preparation method comprises the following steps:
s1, weighing 1kg of nano titanium dioxide, 8kg of humic acid, 30kg of organic compound fertilizer, 58.5kg of straw powder and 2.5kg of cellulase for later use; wherein, the nano titanium dioxide is anatase nano titanium dioxide; the organic compound fertilizer can adopt the existing common nitrogen, phosphorus and potassium compound fertilizers sold in the market, and can be selected according to the actual need for soil restoration.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 8
The embodiment provides an enzyme-catalyzed conditioning agent for soil remediation, and the preparation method comprises the following steps:
s1, weighing 0.5kg of nano titanium dioxide, 10kg of humic acid, 25kg of organic compound fertilizer, 61kg of straw powder and 3.5kg of cellulase for later use; wherein, the nano titanium dioxide is anatase nano titanium dioxide; the organic compound fertilizer can adopt the existing common nitrogen, phosphorus and potassium compound fertilizers sold in the market, and can be selected according to the actual need for soil restoration.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 9
The embodiment provides an enzyme-catalyzed conditioning agent for soil remediation, and the preparation method comprises the following steps:
s1, weighing 1.8kg of nano titanium dioxide, 16kg of humic acid, 35kg of organic compound fertilizer, 46kg of straw powder and 1.2kg of cellulase for later use; wherein, the nano titanium dioxide is anatase nano titanium dioxide; the organic compound fertilizer can adopt the existing common nitrogen, phosphorus and potassium compound fertilizers sold in the market, and can be selected according to the actual need for soil restoration.
S2, mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioner.
Example 10
The embodiment provides an application of the enzyme catalysis modifying agent provided by the embodiment 7 in repairing a hydrocarbon organic matter polluted site in northeast. Specifically, the hydrocarbon organic matter contaminated site is located in a certain northeast region, the contaminated soil contains a large amount of oil sludge mixture discharged from an oil exploitation process, the pollution concentration of the oil sludge mixture is very high, oil sludge is in a black viscous state, the total content of petroleum hydrocarbon contained in the oil sludge contaminated soil is reduced to be below 2% (20000mg/kg) of comprehensive utilization pollution control index of oil-containing sludge in an oil field, and the repairing method is shown as an attached drawing 1 and specifically comprises the following steps:
s1, firstly, taking the polluted soil, removing impurities to remove stones and plant residues, and then, crushing and homogenizing by using a mixer to obtain a sample to be repaired.
S2, carrying out pH measurement on the sample to be repaired, wherein the test result is 7.98, and the pH value does not need to be adjusted; then, 200 kg of sample to be repaired is taken, 4kg of the enzyme catalysis conditioning agent provided in the embodiment 7 is weighed according to the data of pollutant components, equivalent weight and the like detected by the original sample of the polluted soil, and is added into the sample to be repaired and uniformly mixed to obtain the sample to be maintained.
S3, preparing the sample to be maintained into a biological pile, wherein the size of the pile is about 1X 0.8X 0.3 (length, width and height, unit: meter), spraying 20 kilograms of water (properly adjusted according to the water content of polluted soil) to ensure that the saturated water holding capacity of the soil in the sample to be maintained reaches more than 80 percent of a saturated state, and maintaining; wherein, the curing environment temperature is more than 15 ℃, and the ploughing and planing are carried out twice every day; the curing time lasted 10 weeks.
And (3) sampling and detecting the sample to be maintained, wherein the sample to be maintained is maintained, and the specific detection data is shown in table 1 and attached drawing 2.
TABLE 1
As can be seen from the table 1 and the attached figure 2, after 6 weeks of maintenance, the total content of petroleum hydrocarbon in the polluted soil is reduced to be less than 20000mg/kg, which reaches the oilfield industry restoration standard; after 8 weeks of maintenance, the total content of petroleum hydrocarbon is reduced to below 3000mg/kg, which reaches the repair standard of agricultural industry; after curing for 10 weeks, the total content of petroleum hydrocarbon is reduced to below 826mg/kg, which reaches the national construction land screening value repair standard.
Example 11
The embodiment provides an application of the enzyme catalysis modifying agent provided by the embodiment 8 in repairing a hydrocarbon organic matter polluted site in North China. Specifically, the polluted site is located in a certain area in North China, and crude oil is leaked and overflowed to a certain extent due to aging of an oil pipeline, so that pollution to surrounding soil is generated for a certain period. The restoration method is shown as the attached figure 1 and specifically comprises the following steps:
s1, firstly, taking the polluted soil, removing impurities to remove stones and plant residues, and then, crushing and homogenizing by using a mixer to obtain a sample to be repaired.
S2, carrying out pH measurement on the sample to be repaired, wherein the test result is 8.3, and the pH value does not need to be adjusted; then, 300 kg of sample to be repaired is taken, and 4.5 kg of the enzyme catalysis modifying agent provided by the embodiment 8 is weighed according to the data of pollutant components, equivalent weight and the like detected by the original sample of the polluted soil, added into the sample to be repaired, and uniformly mixed to obtain the sample to be maintained.
S3, preparing the sample to be maintained into a biological pile, wherein the size of the pile is about 1X 0.8X 0.4 (length, width and height, unit: meter), spraying 30 kilograms of water (properly adjusted according to the water content of polluted soil) to ensure that the saturated water holding capacity of the soil in the sample to be maintained reaches more than 80 percent of a saturated state, and maintaining; wherein, the curing environment temperature is more than 15 ℃, and the ploughing and planing are carried out twice every day; the curing time lasted 10 weeks.
And (3) sampling and detecting the sample to be maintained, wherein the sample to be maintained is maintained, and the specific detection data is shown in table 2 and attached drawing 3.
TABLE 2
As can be seen from Table 2 and attached figure 3, after 4 weeks of maintenance, the total content of petroleum hydrocarbon has been reduced to be below 20000mg/kg, which reaches the oilfield industry restoration standard; after 7 weeks of maintenance, the total content of petroleum hydrocarbon is reduced to below 3000mg/kg, which reaches the repair standard of agricultural industry; after 9 weeks of maintenance, the total content of petroleum hydrocarbon is reduced to below 826mg/kg, which reaches the national construction land screening value repair standard.
Example 12
The embodiment provides an application of the enzyme catalysis modifying agent provided by the embodiment 9 in the remediation of a hydrocarbon organic polluted site in southwest. Specifically, the polluted site is located in a certain area in the southwest, the polluted soil contains a large amount of ground oil sludge, the total petroleum hydrocarbon content of the polluted soil is 93486mg/kg, the remediation target is to reduce the total petroleum hydrocarbon content of the polluted soil to be below 2% (20000mg/kg) of the comprehensive utilization pollution control index of the oil field oil-containing sludge, and the remediation method is shown as the attached drawing 1 and specifically comprises the following steps:
s1, firstly, taking the polluted soil, removing impurities to remove stones and plant residues, and then, crushing and homogenizing by using a mixer to obtain a sample to be repaired.
S2, carrying out pH measurement on the sample to be repaired, wherein the test result is 8.98, and the pH value does not need to be adjusted; then, 200 kg of a sample to be repaired is taken, and 5kg of the enzyme catalysis conditioning agent provided by the embodiment 9 is weighed according to the data of pollutant components, equivalent weight and the like detected by the original sample of the polluted soil, added into the sample to be repaired, and uniformly mixed to obtain the sample to be maintained.
S3, preparing the sample to be maintained into a biological pile, wherein the size of the pile is about 1X 0.6X 0.3 (length X width X height, unit: meter), spraying 18 kilograms of water (properly adjusted according to the water content of polluted soil) to ensure that the saturated water holding capacity of the soil in the sample to be maintained reaches more than 60 percent of a saturated state, and maintaining; wherein, the curing environment temperature is more than 15 ℃, and the ploughing and planing are carried out twice every day; the curing time lasted 10 weeks.
And (3) sampling and detecting the sample to be maintained, wherein the sample to be maintained is maintained, and the specific detection data is shown in table 3 and attached figure 4.
TABLE 3
As can be seen from Table 3 and attached figure 4, after 7 weeks of maintenance, the total content of petroleum hydrocarbon has been reduced to be below 20000mg/kg, which reaches the oilfield industry restoration standard; after 9 weeks of maintenance, the total content of petroleum hydrocarbon is reduced to below 3000mg/kg, which reaches the repair standard of agricultural industry; after curing for 10 weeks, the total content of petroleum hydrocarbon is reduced to below 826mg/kg, which reaches the national construction land screening value repair standard.
In conclusion, the enzyme catalysis conditioning agent provided by the embodiment of the invention can be used for rapidly repairing complex soil polluted by hydrocarbon organic matters (petroleum), and provides a low-cost green repairing material which is simple and reliable in construction, free of secondary environmental pollution, short in repairing and treating period and free of microbial culture for repairing the soil polluted by the hydrocarbon organic matters, so that the repairing time of the soil polluted by the hydrocarbon organic matters is efficiently shortened, a large amount of funds can be saved for repairing and treating units and owners, secondary pollution can not be generated to a repairing and treating area, the purposes of rapidly repairing and recovering the original property of the soil are really achieved, and the environmental benefit, the economic benefit and the social benefit are very obvious.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. An enzyme-catalyzed conditioning agent for soil remediation, which is characterized by comprising the following components in percentage by mass: 0.2-2% of nano titanium dioxide, 2-20% of humic acid, 15-40% of organic compound fertilizer, 40-80% of straw powder and 1-5% of cellulase, wherein the sum of the mass fractions of the components is 100%.
2. The enzyme-catalyzed conditioning agent for soil remediation of claim 1, wherein the enzyme-catalyzed conditioning agent comprises the following components in mass fraction: 0.5-1% of nano titanium dioxide, 5-15% of humic acid, 20-30% of organic compound fertilizer, 50-72.5% of straw powder and 2-4% of cellulase, wherein the sum of the mass fractions of the components is 100%.
3. The enzyme-catalyzed conditioning agent for soil remediation according to claim 1 or 2, wherein the nano titanium dioxide is anatase nano titanium dioxide.
4. A method for preparing the enzyme catalysis modifying agent according to any one of claims 1 to 3, which comprises the following steps:
weighing nanometer titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase according to the mass fractions of the components for later use;
and mixing the weighed nano titanium dioxide, humic acid, organic compound fertilizer, straw powder and cellulase, and homogenizing to obtain the enzyme catalysis conditioning agent.
5. An enzyme-catalyzed modifying agent prepared by the method of claim 4.
6. Use of an enzymatic conditioner as defined in any one of claims 1 to 3 and 5 for remediation of soil and/or sludge contaminated with hydrocarbon organic compounds.
7. A use according to claim 6, characterised in that it comprises the following steps:
after the soil and/or oil sludge to be repaired is subjected to impurity removal treatment, crushing treatment and homogenization treatment are carried out to obtain a sample to be repaired;
controlling the pH value of a sample to be repaired to be 5-9, and then mixing the sample with the enzyme catalysis modifying agent to obtain a sample to be maintained;
and adding water into the sample to be maintained to enable the saturated water holding capacity of the sample to be maintained to be not less than 60%, and maintaining to finish the repairing treatment of the soil and/or the oil sludge to be repaired.
8. A use according to claim 7, wherein in said step, the mass of the enzymatic conditioning agent is between 0.5% and 2.5% of the mass of the sample to be repaired.
9. A use according to claim 7, wherein in said step, the curing temperature is not lower than 15 ℃.
10. The use of claim 9, wherein in the step of curing, the curing time is 4-10 weeks.
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030129735A1 (en) * | 2002-01-08 | 2003-07-10 | Moorhead Elliot I. | Biocatalytic method for remediation of soil, water and air. |
| US6617150B1 (en) * | 1999-11-15 | 2003-09-09 | Geovation Technologies, Inc. | Solid-chemical composition for biodegradation comprising plant fiber-containing material and enzymes |
| CN101632900A (en) * | 2009-08-11 | 2010-01-27 | 孙祥章 | High-efficiency gas purification bioreactor based on complex enzyme immobilization technology and purification method thereof |
| CN102351321A (en) * | 2011-09-06 | 2012-02-15 | 佛山市合璟节能环保科技有限公司 | Composite biological enzyme preparation for deweighting treatment of domestic sludge, and production and application method thereof |
| US20140273150A1 (en) * | 2013-03-15 | 2014-09-18 | Janet Angel | Compositions and Methods of Use |
| CN105779528A (en) * | 2014-12-22 | 2016-07-20 | 中国石油化工股份有限公司 | Lignocellulose continuous enzymatic hydrolysis and synchronous saccharification-fermentation method |
| CN107470334A (en) * | 2017-07-03 | 2017-12-15 | 浙江海洋大学 | A kind of compounding renovation agent for being used to clear up petroleum-polluted soil |
| CN108003879A (en) * | 2017-10-31 | 2018-05-08 | 浦江县合洪园艺研发有限公司 | A kind of modifying agent for chemical pollution soil |
| CN108609812A (en) * | 2018-06-26 | 2018-10-02 | 浙江国邦药业有限公司 | A kind of method for processing organic wastewater |
| CN109134146A (en) * | 2018-09-11 | 2019-01-04 | 河北麦森钛白粉有限公司 | A kind of soil-repairing agent containing loading nano-titania |
| CN110387244A (en) * | 2019-07-29 | 2019-10-29 | 山东胜伟盐碱地科技有限公司 | A kind of organic polluted soil renovation agent and its production method and application |
-
2020
- 2020-04-22 CN CN202010322001.3A patent/CN111500291B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6617150B1 (en) * | 1999-11-15 | 2003-09-09 | Geovation Technologies, Inc. | Solid-chemical composition for biodegradation comprising plant fiber-containing material and enzymes |
| US20030129735A1 (en) * | 2002-01-08 | 2003-07-10 | Moorhead Elliot I. | Biocatalytic method for remediation of soil, water and air. |
| CN101632900A (en) * | 2009-08-11 | 2010-01-27 | 孙祥章 | High-efficiency gas purification bioreactor based on complex enzyme immobilization technology and purification method thereof |
| CN102351321A (en) * | 2011-09-06 | 2012-02-15 | 佛山市合璟节能环保科技有限公司 | Composite biological enzyme preparation for deweighting treatment of domestic sludge, and production and application method thereof |
| US20140273150A1 (en) * | 2013-03-15 | 2014-09-18 | Janet Angel | Compositions and Methods of Use |
| CN105779528A (en) * | 2014-12-22 | 2016-07-20 | 中国石油化工股份有限公司 | Lignocellulose continuous enzymatic hydrolysis and synchronous saccharification-fermentation method |
| CN107470334A (en) * | 2017-07-03 | 2017-12-15 | 浙江海洋大学 | A kind of compounding renovation agent for being used to clear up petroleum-polluted soil |
| CN108003879A (en) * | 2017-10-31 | 2018-05-08 | 浦江县合洪园艺研发有限公司 | A kind of modifying agent for chemical pollution soil |
| CN108609812A (en) * | 2018-06-26 | 2018-10-02 | 浙江国邦药业有限公司 | A kind of method for processing organic wastewater |
| CN109134146A (en) * | 2018-09-11 | 2019-01-04 | 河北麦森钛白粉有限公司 | A kind of soil-repairing agent containing loading nano-titania |
| CN110387244A (en) * | 2019-07-29 | 2019-10-29 | 山东胜伟盐碱地科技有限公司 | A kind of organic polluted soil renovation agent and its production method and application |
Non-Patent Citations (3)
| Title |
|---|
| 崔春: "《食物蛋白质控制酶解技术》", vol. 1, 30 June 2018, 中国轻工业出版社, pages: 26 * |
| 张矢: "《植物原料水解工艺学》", vol. 1, 30 June 1994, 中国林业出版社, pages: 40 * |
| 郑西来: "《土壤-地下水系统石油污染原理及应用研究》", vol. 1, 30 September 2004, 地质出版社, pages: 23 * |
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