CN102338806A - Novel random multi-category full-automatic immunoassay system - Google Patents
Novel random multi-category full-automatic immunoassay system Download PDFInfo
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- CN102338806A CN102338806A CN2010102349095A CN201010234909A CN102338806A CN 102338806 A CN102338806 A CN 102338806A CN 2010102349095 A CN2010102349095 A CN 2010102349095A CN 201010234909 A CN201010234909 A CN 201010234909A CN 102338806 A CN102338806 A CN 102338806A
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- immunobead
- reaction
- detection
- reaction cup
- cup
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- 238000003018 immunoassay Methods 0.000 title abstract 6
- 238000006243 chemical reaction Methods 0.000 claims abstract description 58
- 238000001514 detection method Methods 0.000 claims abstract description 30
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 19
- 238000004140 cleaning Methods 0.000 claims abstract description 15
- 230000035945 sensitivity Effects 0.000 claims abstract description 9
- 230000000694 effects Effects 0.000 claims abstract description 3
- 238000012360 testing method Methods 0.000 claims description 20
- 230000036039 immunity Effects 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 239000000427 antigen Substances 0.000 claims description 6
- 102000036639 antigens Human genes 0.000 claims description 6
- 108091007433 antigens Proteins 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 239000007769 metal material Substances 0.000 claims 2
- 239000012295 chemical reaction liquid Substances 0.000 claims 1
- 238000009434 installation Methods 0.000 claims 1
- 239000000696 magnetic material Substances 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 229920003023 plastic Polymers 0.000 claims 1
- 239000004033 plastic Substances 0.000 claims 1
- 239000011435 rock Substances 0.000 claims 1
- 239000011324 bead Substances 0.000 abstract description 8
- 239000000376 reactant Substances 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 2
- 229940127121 immunoconjugate Drugs 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 9
- 230000008859 change Effects 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002796 luminescence method Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Abstract
The invention discloses a novel random multi-category full-automatic immunoassay system. The novel random multi-category full-automatic immunoassay system comprises immunomagnetic beads, reagents and an automatic analyzer. A detection flow adopting the novel random multi-category full-automatic immunoassay system comprises the following steps of putting needed immunomagnetic beads into a reaction cup according to detection categories, adding respectively a sample, reagents and the like into the reaction cup according to a detection program, wherein before addition of each one of the reagents, the immunomagnetic beads and the reaction cup all are cleaned thoroughly, and detecting signals such as light and the like produced immunoconjugates so that a detection purpose is realized. A reaction disk can control a temperature according to detection temperature requirements. During cleaning, the novel random multi-category full-automatic immunoassay system can produce a magnetic field to enable the immunomagnetic beads to suspend or to float up and down, so that good cleaning effects are obtained and a cleaning needle can reach directly and easily to the bottom of the reaction cup to remove residual liquid by suction. After the detection flow is finished, the immunomagnetic beads are taken out by an immunomagnetic beads a removal arm and are abandoned, and the reaction cup is cleaned up and is utilized for the next detection flow. The novel random multi-category full-automatic immunoassay system can push forward immunomagnetic beads or a shakeable reaction disk by a magnetic force to promote full contact between reactants so that reaction sensitivity is improved.
Description
One, technical field
A kind of novel optional at random multinomial order automatic immunity of formula system is that a kind of can optionally at random synchronously the detection reaches dozens of immunity inspection project and can accomplish a kind of full-automatic instrument of measuring, and belongs to medical science and immunology detection self-reacting device field.
Two, background technology
Immunology detection is very important aspect medical diagnosis on disease, is being widely used aspect the medicals diagnosis on disease such as infectious disease, tumour, endocrine, and a plurality of fields such as this external agricultural, pharmacy, food, environment and life science also are widely used.In actual immunology detection, often need carry out a plurality of even reach the detection of tens of immune projects same sample.And the immune appearance project organization of existing full-automation bulky complex, complex operation, online test item are few, and speed is slow, are generally batch type and detect the test item very flexible.Though little magnetic bead of employing is also arranged at present or moulds the immune appearance of ball formula; Compare with a kind of novel optional at random multinomial order automatic immunity of formula system of the present invention: exist testing process different; Online project is difficult to a large amount of expansions; Reagent is preserved inconvenience, the problem more than institute's consumptive material/reagent that consumes, so have certain defective.A kind of novel optional at random multinomial order automatic immunity of the formula system that the present invention designed adopts a kind of new project organization; Is characteristics with simple and direct testing process with novel carrier format; This system has: simple in structure, required reagent/consumptive material is of less types, and reagent is preserved more convenient, reliable; The test item expansion is easy, and system also has the accelerated reaction process function and plurality of advantages such as more flexible are used in free control detection sensitivity.
Three, summary of the invention
A kind of novel optional at random multinomial order automatic immunity of the formula system of the present invention's design, the immunobead with magnetic, reagent parts such as (labelled antibody, antiantibody, washing agent) and full-automatic analyser two parts that comprise with antigen or antibody sandwich are formed jointly.Instrument part primary structure has: add reagent arm (1), reagent dish (2), immunobead taking-up arm (3), detecting device (4), cleaning arm (5), sample disc (6), add sample arm (7), immunobead dish (8), reaction tray primary structure parts such as (9).System's testing process is the testing process of the design according to the present invention: at first according to required test item needed immunobead is put into and detected cup; Under instrument control, add sample, reagent etc. according to trace routine respectively successively then, after each reaction is accomplished, reaction cup and immunobead are thoroughly cleaned.Reach testing goal through detecting the immune conjugate luminous signal at last.Reaction tray requires regulating and controlling temperature according to detected temperatures; Instrument fluctuates the immunobead suspension to reaction cup generation magnetic field in cleaning process, to reach excellent cleaning effect and to be convenient to the through reaction cup of cleaning needle bottom exhaustion debris; Detect the end back and the immunobead taking-up is abandoned, can begin new detection circulation after again reaction cup being cleaned up again by immunobead taking-up arm.System in detection, promotes immunobead through magnetic force or the vibrations reaction tray promotes that reactant fully contacts, and improves reaction sensitivity.Used immunobead quantity when system can also require selection change immunobead to detect according to the required sensitivity of test item, or size or surface structure raising coated antibody/antigen amount through changing immunobead, and the increase surface in contact is long-pending to improve detection sensitivity.
Four, description of drawings
Accompanying drawing is the structural representation of the multinomial order automatic immunity of a kind of novel optional at random formula system.
Among the figure, 1, add the reagent arm, 2, the reagent dish, 3, immunobead takes out arm, 4, reaction tray, 5, reaction cup, 6, detecting device, 7, cleaning arm, 8, clean-out system, 9, sample disc, 10, add sample arm, 11, change the Jian Zui district, 12, the immunobead dish.
Five, embodiment
Below in conjunction with accompanying drawing the present invention is done further description:
A kind of novel optional at random multinomial order automatic immunity of the formula system of the present invention's design; This system adopts the baton round with magnetic as solid phase carrier; This baton round is made not synantigen or antibody sandwich is treated as immunobead according to the testing goal needs; Utilize the antigen-antibody reaction principle that antibody to be detected or antigen are combined immunobead surface so far; Through biotin and Avidin amplification system, detect luminous signal and reach testing goal with enzyme-catalyzed chemical luminescence method or direct mark chemiluminescent substance.Whole process is full-automatic to be accomplished.Prepare various immunobeads and be placed on respectively in the jar special (bottle), Open Side Down during use is placed on the immunobead dish (12) ad hoc on the instrument, and an immunobead dish can be placed 20 above immunobead jars.The may command opening is arranged under the immunobead jar; Open immunobead jar mouth according to system's detection needs; Immunobead is from top to bottom slipped in the reaction cup (5) on the reaction tray (4) automatically, add sample arm (10) subsequently and in reaction cup (5), add test sample and carry out immune association reaction; Cleaning arm (7) to reaction cup (5), immunobead cleans and the residual liquid that exhausts; Add reagent arm (1) by system then and in reaction cup (5), add reagent in order according to detecting needs.After the reaction of each interpolation novel agent is accomplished; Cleaning arm will clean and blot and just add a kind of reagent down in the clean cup after the debris reaction cup (5); In cleaning process, be suspension owing to the action of a magnetic field immunobead; Can make cleaning more thorough, and also be convenient at the bottom of the through reaction cup of aspirating needle raffinate thoroughly exhausted.The luminous intensity that is read in the reaction cup (5) by detecting device (6) is at last made testing result.Detection finishes the back and abandons it by the magnetic force probe with the immunobead taking-up and with it by immunobead taking-up arm (3).Reaction cup through cleaning, can begin new testing process again again.System in detection, promotes immunobead through magnetic force or the vibrations reaction tray promotes that reactant fully contacts, and improves reaction sensitivity.Used immunobead quantity when system can also require to select to change the immunobead detection according to the required sensitivity of test item.
Claims (9)
1. the novel optional at random multinomial order automatic immunity of formula system; It is characterized in that: the carrier that this system detects employed envelope antigen or antibody is the sphere (immunobead) that diameter 1～15mm pearl has magnetic, and antibody, antigen coated plastics or other materials are processed, its heart is equipped with or mictomagnetism or metal material by being convenient on the immunobead surface.The various immunobeads that prepare are contained in respectively in the immunobead jar of standard; The immunobead jar mouth that immunobead will be housed when prepare detecting is to being placed down on the immunobead dish on the custom-designed position; Each immunobead jar mouth respectively with the immunobead dish on immunobead discharge the control gear close installation and combine; Can control immunobead jar opening automatically through this mechanism's instrument, realize that immunobead has falling of control.And the immunobead dish is arranged on the position that is higher than reaction cup on the reaction tray, and two local intersecting of dish, the immunobead that therefore falls can directly get in the reaction cup of appointment.Instrument is put into reaction cup according to the needs of test item with immunobead, under the main testing process of this system (reaction is hatched, washs, added reagent, removes reaction reagent, detection etc.) is assisted by the corresponding mechanism that is provided with around the circular reaction tray, accomplish in the reaction cup of on reaction tray, loading.
2. a kind of novel optional at random multinomial order automatic immunity of formula system according to claim 1; It is characterized in that: this system detects employed antigen or its surface of antibody sandwich used carrier immunobead can be smooth; Also can be equally distributed projection to be arranged to increase surface area; And immunobead contains metal or magnetic material, makes it have magnetic.
3. a kind of novel optional at random multinomial order automatic immunity of formula system according to claim 1; It is characterized in that: before the instrument work not the immunobead of isolabeling leave in the different immunobead jars respectively and mode is placed in the immunobead dish that is higher than reaction cup so that Open Side Down; The immunobead dish is designed with immunobead release control gear for each immunobead jar mouth, requires each control to emit one or more immunobeads according to test item and directly falls in the reaction cup.
4. a kind of novel optional at random multinomial order automatic immunity of formula system according to claim 1; It is characterized in that: the employed reaction cup of this system is conformance to standard, no project specificity, and do not have special biology in the reaction cup in advance, biochemical substances encapsulates or mark.
5. a kind of novel optional at random multinomial order automatic immunity of formula system according to claim 1; It is characterized in that: after detecting completion; Used immunobead by one have magnetic immunobead take out and to abandon after arm takes out; Can put into new immunobead again after the reaction cup that instrument will be accomplished the detection task cleans up and the detection background optical signal value meets the requirements, begin new detection and circulate, or the detection cup that also can more renew begin new detection.
6. a kind of novel optional at random multinomial order automatic immunity of formula system according to claim 1; It is characterized in that: when reaction cup is washed; Instrument is opened and is arranged on the magnetic force generating means under the reaction cup; Immunobead boosted be suspended state, reach and make cleaning more thorough, also be convenient to the through reaction cup of cleaning needle (aspirating needle) bottom simultaneously reaction cup bottom debris is drained clean purpose.
7. a kind of novel optional at random multinomial order automatic immunity of formula system according to claim 1; It is characterized in that: instrument also utilizes magnetic force, reaction tray means such as rock controlledly to make sample, reagent and immunobead relative motion; It is fully contacted; Reach and add fast response (immunity combination, biochemistry, chemical reaction etc.) speed, or make the more slap-up effect of reaction.
8. a kind of novel optional at random multinomial order automatic immunity of formula system according to claim 1; It is characterized in that: for improving detection sensitivity; Can be according to the difference of institute's test item sensitivity requirement; Use the immunobead of different sizes, or select to use one or more immunobeads to carry out reaction detection.
9. a kind of novel optional at random multinomial order automatic immunity of formula system according to claim 1; It is characterized in that: its detection can directly be accomplished in reaction cup, also can reaction sphere or end reaction liquid be transferred to completion detection in the other detection cup through special arrangement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010234909 CN102338806B (en) | 2010-07-23 | 2010-07-23 | Novel random multi-category full-automatic immunoassay system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010234909 CN102338806B (en) | 2010-07-23 | 2010-07-23 | Novel random multi-category full-automatic immunoassay system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102338806A true CN102338806A (en) | 2012-02-01 |
| CN102338806B CN102338806B (en) | 2013-09-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010234909 Expired - Fee Related CN102338806B (en) | 2010-07-23 | 2010-07-23 | Novel random multi-category full-automatic immunoassay system |
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| Country | Link |
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| CN (1) | CN102338806B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102375058A (en) * | 2010-08-26 | 2012-03-14 | 南京神州英诺华医疗科技有限公司 | Novel random optional multi-item full-automatic immunoassay system |
| CN103808923A (en) * | 2013-11-25 | 2014-05-21 | 珠海迪尔生物工程有限公司 | Movable type magnetic separation fluorescence immunoassay detection and analysis method and movable type magnetic separation fluorescence immunoassay detection and analysis device |
| CN104345158A (en) * | 2013-07-30 | 2015-02-11 | 苏州浩欧博生物医药有限公司 | Automatic analysis apparatus and automatic analysis method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5828450A (en) * | 1995-07-19 | 1998-10-27 | Kyoto Dai-Ichi Kagaku Co., Ltd. | Spectral measuring apparatus and automatic analyzer |
| CN1563989A (en) * | 2004-03-26 | 2005-01-12 | 北京博奥生物芯片有限责任公司 | Method of detecting small molecule compound and its special biochip |
| CN1763532A (en) * | 2004-10-19 | 2006-04-26 | 深圳依诺金生物科技有限公司 | Randomly combined multi-item parallel immune detection method and kit therefor |
| CN1781020A (en) * | 2003-04-25 | 2006-05-31 | 捷时雅株式会社 | Biochip and biochip kit, and method of producing the same and method of using the same |
| CN201237594Y (en) * | 2008-04-16 | 2009-05-13 | 北京科美东雅生物技术有限公司 | Chemical luminescence immunity analysis reagent box for combined detection of multiple infectious disease items |
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2010
- 2010-07-23 CN CN 201010234909 patent/CN102338806B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5828450A (en) * | 1995-07-19 | 1998-10-27 | Kyoto Dai-Ichi Kagaku Co., Ltd. | Spectral measuring apparatus and automatic analyzer |
| CN1781020A (en) * | 2003-04-25 | 2006-05-31 | 捷时雅株式会社 | Biochip and biochip kit, and method of producing the same and method of using the same |
| CN1563989A (en) * | 2004-03-26 | 2005-01-12 | 北京博奥生物芯片有限责任公司 | Method of detecting small molecule compound and its special biochip |
| WO2005093419A1 (en) * | 2004-03-26 | 2005-10-06 | Capitalbio Corporation | Methods and biochips for detecting small molecule compounds |
| CN1763532A (en) * | 2004-10-19 | 2006-04-26 | 深圳依诺金生物科技有限公司 | Randomly combined multi-item parallel immune detection method and kit therefor |
| CN201237594Y (en) * | 2008-04-16 | 2009-05-13 | 北京科美东雅生物技术有限公司 | Chemical luminescence immunity analysis reagent box for combined detection of multiple infectious disease items |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102375058A (en) * | 2010-08-26 | 2012-03-14 | 南京神州英诺华医疗科技有限公司 | Novel random optional multi-item full-automatic immunoassay system |
| CN104345158A (en) * | 2013-07-30 | 2015-02-11 | 苏州浩欧博生物医药有限公司 | Automatic analysis apparatus and automatic analysis method |
| CN103808923A (en) * | 2013-11-25 | 2014-05-21 | 珠海迪尔生物工程有限公司 | Movable type magnetic separation fluorescence immunoassay detection and analysis method and movable type magnetic separation fluorescence immunoassay detection and analysis device |
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| Publication number | Publication date |
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| CN102338806B (en) | 2013-09-04 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20120224 Address after: 211135 Nanjing, Jiangning, Qilin street, Po Shan Road, No. 7 Applicant after: Nanjing Sinnowa Medical Science & Technology Co., Ltd. Address before: 211135, Baoshan Road, Tangshan District, Jiangsu, Nanjing, No. 7, Jiangning Applicant before: Nanjing Sinnowa Medical Science & Technology Co., Ltd. |
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| CB02 | Change of applicant information |
Address after: 211135 Nanjing, Jiangning, Qilin street, Po Shan Road, No. 7 Applicant after: SINNOWA MEDICAL SCIENCE & TECHNOLOGY CO., LTD. Address before: 211135 Nanjing, Jiangning, Qilin street, Po Shan Road, No. 7 Applicant before: Nanjing Sinnowa Medical Science & Technology Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: NANJING SINNOWA MEDICAL SCIENCE + TECHNOLOGY CO., LTD. TO: JIANGSU SINNOWA MEDICAL SCIENCE + TECHNOLOGY CO., LTD. |
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Granted publication date: 20130904 Termination date: 20200723 |
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| CF01 | Termination of patent right due to non-payment of annual fee |