CN102319059B - Near-infrared fluorescence imaging surgery guide device and application thereof - Google Patents
Near-infrared fluorescence imaging surgery guide device and application thereof Download PDFInfo
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- CN102319059B CN102319059B CN 201110332712 CN201110332712A CN102319059B CN 102319059 B CN102319059 B CN 102319059B CN 201110332712 CN201110332712 CN 201110332712 CN 201110332712 A CN201110332712 A CN 201110332712A CN 102319059 B CN102319059 B CN 102319059B
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Abstract
The invention relates to a near-infrared fluorescence imaging surgery guide device and an application thereof. The device comprises a near-infrared laser light source, a light collection optical system, a visual light filter, a near-infrared light filter, a color video camera, a near-infrared video camera and a terminal display. The device can conveniently perform position guide and morphic display functions in the process of a lymph excision or inactivation surgery, and meanwhile can be used for analyzing and evaluating the effect of the lymph excision or inactivation surgery. The device is favorable to improving surgery efficiency and accuracy and accumulating surgery treatment experience.
Description
Technical field
The present invention relates to a kind of clinical operation guiding device and application thereof of near-infrared fluorescence imaging, specifically, the present invention relates to a kind of device and the application thereof that the position is guided be carried out in the excision such as lymph or deactivation position.
Background technology
Come the method for labelling biomolecule in the biomedical research field, successfully to use the several years with radioactive tracer and fluorescent dye, but in the body clinical medicine imaging field of reality, the application of fluorescent probe in clinical research but is confined to the inspection of external dyeing tissue slice mostly.Until the later stage eighties 20th century, some researcheres are attempted the as a comparison agent of fluorescent dye of injection of exogenous in the organism, by Noninvasive or in the optical measurement means of peeping, in lesion detection, distinguish morbid state and normal structure.In recent years, obtained great success in the method for carrying out in the tumor imaging in vivo using fluorescence indicator as exogenous contrast medium with near-infrared.
In the prior art; when carrying out the inadvertent closure of live body lymph (human body or animal body); surgical staff often can be injected fluorescent contrast agent in blood, contrast agent by Lymphatic, can be discharged along with the circulation of blood again after after a while in the blood circulation process simultaneously.Contrast agent can send the near infrared light with the near infrared light different-waveband that shines when being subject to the near infrared ray irradiation, then by gathering the superelevation sensitivity camera of near infrared light, observe with monitor, with the position of correct grasp lymph body, correctly carry out the resection operation of lymph with the guided surgery personnel.
Figure 1 shows that carrying out the lymph position of sending fluorescence and the device that form is observed as the assisted surgery personnel of having emerged at present.This device comprises near-infrared laser luminous source, video camera, optical filter, display.Near infrared light is behind the lymph body that has absorbed fluorescent contrast agent, the fluorescent contrast agent excitation-emission has gone out the near infrared light different from the irradiation optical band, get access to the near infrared light that fluorescent contrast agent is launched by optical filter, then the near infrared light image that obtains by camera acquisition shows that by display the near-infrared image that camera acquisition arrives supplies surgical staff to observe.But there is following shortcoming in described device:
(1), this device only can be observed and show the area image of the blood that contains fluorescent contrast agent and can not show the image that does not excite the position that does not contain fluorescent contrast agent blood.Can not see like this situation of the operative region of the integral body that comprises the lymph body with regard to causing surgical staff can only see the lymph body situation of irradiated site, if watch the overall condition of whole operative region, just need to close the LASER Light Source of irradiation, but so just can't see again and wish cut portion bit position and form, surgical staff needs repeatedly shining near infrared light and closing repeatedly switching between the near infrared light for this reason, it is very inconvenient to use, and causes the puzzlement in operation process.
(2), this device provides by display direct observation operative region and has absorbed the lymph body means of fluorescent contrast agent, but the size and geometric that can not excise front and back to position and form, the operative region endolymph body of lymph body etc. carries out quantitative record and analysis, evaluation between resection operation and final therapeutic effect can not quantitatively be provided, be unfavorable for the surgical staff summing up experience, mutually exchange, improve treatment level.
Summary of the invention
In order to solve existing above-mentioned technical problem in the prior art, the invention provides a kind of guide device of near-infrared fluorescence angiography surgery, device of the present invention can be realized carrying out easily the function that location guide and form show in the process that the lymph body excises or deactivation is performed the operation; Also can be used in simultaneously assay is carried out in lymph excision or deactivation surgical effect.Device of the present invention is very helpful for raising operation efficient, accuracy, accumulation experience of surgical tool.
A first aspect of the present invention relates to a kind of guide device of near-infrared fluorescence angiography surgery, comprising: near-infrared laser luminous source, light are collected optical system, visible light light filter, near infrared filter device, color video camera, near-infrared video camera and terminal display; It is characterized in that described near-infrared laser luminous source is used for the lymph body that indocyanine green ICG was injected in irradiation, excites to make it send near-infrared fluorescent; Described light is collected optical system and is comprised light collector and beam splitter, blood was stimulated and other shine the light of lymph body back reflection under the fluorescence launched and the surgical environments when described light collector be used for to be collected excitation source and shone the lymph body that has absorbed ICG, and the light that described beam splitter is collected light collector is divided into the identical light of two-way; Described visible light light filter filters the visible light that obtains visible wavelength range; Described near infrared filter device filters the fluorescence of the near-infrared wavelength that obtains near infrared wavelength region; Described color video camera reception visible light light filter filters the visible light that obtains and obtains near-infrared image or video; Described near-infrared video camera reception near infrared filter device filters the fluorescence that obtains and obtains visible images or video.
In this guide device of near-infrared fluorescence angiography surgery, also comprise video capture card and analytical system.This video capture card is used for and will be converted into the digital signal that can carry out stored digital and processing from image or the video signal of video camera; This analytical system can realize the digital signal of video capture card collection is carried out the recording and multiple morphological parameters analysis for clinical and research and teaching, calculate and report of storage, multiple format of various ways.
In this guide device of near-infrared fluorescence angiography surgery, described terminal display is used for directly showing near-infrared image and the visible images from camera acquisition, and two kinds of images can show respectively, show side by side simultaneously or Overlapping display.
In this guide device of near-infrared fluorescence angiography surgery, described terminal display is used for showing system storage by analysis or the digital signal of recording, perhaps directly show near-infrared image and visible images from camera acquisition, two kinds of images can show respectively, show side by side simultaneously or Overlapping display.
In this guide device of near-infrared fluorescence angiography surgery, described near-infrared laser luminous source is near-infrared luminous source or the adjustable near-infrared laser luminous source of wavelength of fixed wave length.Preferably, the wavelength of described near-infrared laser luminous source is 805nm.
In this guide device of near-infrared fluorescence angiography surgery, the visible light that described visible light light filter permission wave-length coverage is 400-700nm passes through; The infrared ray that described near infrared filter device permission wave-length coverage is 820-850nm passes through.
In this guide device of near-infrared fluorescence angiography surgery, described video capture card is integrated in the computer of analytical system or is integrated in the video camera.
A second aspect of the present invention relates to the application of above-mentioned guide device of near-infrared fluorescence angiography surgery, is used for excision or deactivation operation process, and location guide and effect assessment are carried out in the excision such as lymph body or deactivation position.
The operating principle of guide device of near-infrared fluorescence angiography surgery of the present invention: described near-infrared laser luminous source is near-infrared luminous source or the adjustable near-infrared laser luminous source of wavelength of fixed wave length; Blood was stimulated and other shine the reflected light behind the lymph body under the fluorescence launched and the surgical environments when described light collector be used for to be collected excitation source and shone the lymph body that has absorbed ICG; The light that described beam splitter is collected light collector is divided into the identical light of two-way; The visible light that described visible light light filter permission wave-length coverage is 400-700nm passes through; The infrared ray that described near infrared filter device permission wave-length coverage is 820-850nm passes through; Then have the optical filter of different filtering characteristics through two respectively and carried out the collection of optical signal by video camera, obtain respectively under the visible light and near infrared light under video image, two kinds of video images that obtain can be directly at display device difference or Overlapping display, intuitively observe for surgical staff, carry out digitized by the data collection and analysis system simultaneously, the demonstration of carrying out respectively on computers or superposeing, record and carry out analysis and the report of different morphological data.
Compared with prior art, the present invention has following useful technique effect:
(1) adopted the two-way video camera to gather simultaneously visible light coloured image and near-infrared fluorescent image from operative region, and directly with behind the fluorescence image processing with specific color addition on the coloured image of visible light, especially we have broken through usual thinking and have adopted the beam splitter that light is divided into the identical light of two-way, rather than light are divided in advance the beam splitter of near infrared light and visible light; Thereby can guarantee that visible light coloured image and near-infrared fluorescent image can directly superpose and can not produce position deviation, also not have the irradiation blind area; And if according to common operational approach, use is divided into light the beam splitter of near infrared light and visible light in advance, the visible light coloured image that obtains and near-infrared fluorescent image can produce position deviation when directly superposeing, thereby bring significant error for follow-up operation; Also can there be the blind area in the image that obtains that superposes in addition.
(2) we find by a large amount of simulation tests, adopting the wavelength of near-infrared laser luminous source is the fluorescence of 805nm, can be so that absorbing the wavelength overwhelming majority of the near-infrared fluorescent light that the lymph body of ICG excites concentrate in the scope of 825-840nm nanometer, thus utilize wave-length coverage can record to greatest extent the positional information of the lymph body that absorbs ICG for the near infrared filter device of 820-850nm; Accuracy and the resolution of the image that obtains have been improved.
(3) adopt device of the present invention to get situation so that surgical staff can be seen the integral body of field of operation and lymph body wherein simultaneously, farthest adapt to watching and thinking habit of surgical staff, promote operation efficient and correctness.But also the size and geometric that can excise front and back to position and form, the operative region endolymph body of lymph body etc. carry out quantitative record and analysis, evaluation between resection operation and final therapeutic effect quantitatively is provided, be unfavorable for the surgical staff summing up experience, mutually exchange, improve treatment level.
Description of drawings
Fig. 1: near-infrared fluorescent operation guiding device block chart of the prior art;
Fig. 2: the sketch map of the near-infrared fluorescent operation guiding device of one embodiment of the invention;
Fig. 3: the near-infrared absorption rate of the indocyanine green ICG of use, fluorescent radiation rate and wavelength curve;
Fig. 4: wavelength and the transmittance curve of the near infrared filter mirror that one embodiment of the invention is used;
Fig. 5: wavelength and the transmittance curve of the visible light filter lens that one embodiment of the invention is used.
The implication of Reference numeral representative is respectively among the figure: 0-comprises the lymph body of injecting ICG blood; 1-near-infrared laser light source; 2-visible illumination light source; 3-light is collected optical system; The 4-beam splitter; 5-visible light light filter; 6-near infrared filter device; The 7-color video camera; 8-near-infrared video camera; The 9-terminal display; The 10-analytical system.
The specific embodiment
Below in conjunction with accompanying drawing and specific embodiment technical scheme of the present invention is described in detail, but accompanying drawing and specific embodiment be not as the restriction to patent of the present invention.
As shown in Figure 2, the present invention proposes a kind of for excision or deactivation operation process, the guide device of near-infrared fluorescence angiography surgery that the position is guided and effect is estimated is carried out at the excision positions such as lymph, it comprises a near-infrared laser luminous source, can launch the near-infrared laser that wavelength is 805nm, 1500mW; Near-infrared laser shines and has absorbed the surgical object surface of containing indocyanine green ICG blood, and ICG is stimulated and penetrates the near infrared light of 835nm.The radiation of visible light that exists in the surgical environments produces reflected light and scattered light behind the operation body, the light that has mixed 805nm near infrared light, 835nm near infrared light, visible light is divided into the identical light of two bundles by spectroscope and arrives visible light light filter and near infrared filter device, the transmitance of optical filter and wavelength curve are shown in Fig. 4,5, it is that the 835nm bandwidth is that the near infrared light of 20nm wave band passes through that near infrared filter allows centre wavelength, and it is that the visible light of 400nm-700nm passes through that visible filter allows wave-length coverage; After filtration the mating plate removal of filtering the near infrared light of the following wavelength of 820nm (805nm and visible light) and the visible light that only comprises the 400-700nm wave band enter respectively near-infrared video camera and color video camera; Video camera is connected to terminal display after optical signal is converted into video electrical signal, is connected to simultaneously the video signal input terminal of analytical system, the video signal of simulation is converted into the view data of numeral for its built-in video capture card.Method by software realize to digitized view data store, record, calculate, analyze, the processing such as report.
The model of near infrared laser is: DH-NIR805,1500mW, 805nm; Spectroscope customizes to optical coating company separately according to the dimensional requirement of industrial design; Near-infrared and color video camera CCD all adopt SONY low-light (level) ICX639AK chip; Monitor is general LCD TV; Image pick-up card model in the data collection and analysis system is DH321.
By adopting this experimental program, can see simultaneously during operation that the lymph body that needs to excise on the true colored operation volume image that is added to gets vision-mix, for determining that accurate location and the form of lymph body on the operation body has intuitively effect, easily guided surgery personnel high efficiency, finish excision or deactivation operation exactly, and can record perioperatively and operation process, and finish quantitatively the evaluation analysis of surgical effect.
The above; only be the preferred embodiments of the present invention; can not be interpreted as limiting scope of the present invention with this, the embodiment of all distortion that is equal to of making in the claimed scope of claims of the present invention and change is all in the present invention's scope required for protection.
Claims (4)
1. a guide device of near-infrared fluorescence angiography surgery is collected optical system, visible light light filter, near infrared filter device, color video camera, near-infrared video camera, terminal display, video capture card and analytical system by near-infrared laser luminous source, light and is formed; It is characterized in that described near-infrared laser luminous source is used for the lymph body that indocyanine green was injected in irradiation, excites to make it send near-infrared fluorescent; Described light is collected optical system and is comprised light collector and beam splitter, blood was stimulated and other shine the light of lymph body back reflection under the fluorescence launched and the surgical environments when described light collector be used for to be collected excitation source and shone the lymph body that has absorbed indocyanine green, and the light that described beam splitter is collected light collector is divided into the identical light of two-way; Described visible light light filter filters the visible light that obtains visible wavelength range; Described near infrared filter device filters the fluorescence of the near-infrared wavelength that obtains near infrared wavelength region; Described color video camera reception visible light light filter filters the visible light that obtains and obtains visible images or video; Described near-infrared video camera reception near infrared filter device filters the fluorescence that obtains and obtains near-infrared image or video; Described video capture card is used for and will be converted into the digital signal that can carry out stored digital and processing from image or the video signal of video camera; This analytical system can realize the digital signal of video capture card collection is carried out the recording and multiple morphological parameters analysis for clinical and research and teaching, calculate and report of storage, multiple format of various ways; Described terminal display is used for showing system storage by analysis or the digital signal of recording; Perhaps directly show near-infrared image and visible images from camera acquisition, two kinds of images can show respectively, show side by side simultaneously or Overlapping display.
2. guide device of near-infrared fluorescence angiography surgery according to claim 1, the wavelength that it is characterized in that described near-infrared laser luminous source is 805nm; The infrared ray that described near infrared filter device permission wave-length coverage is 820-850nm passes through.
3. guide device of near-infrared fluorescence angiography surgery according to claim 1 is characterized in that the visible light that described visible light light filter permission wave-length coverage is 400-700nm passes through.
4. guide device of near-infrared fluorescence angiography surgery according to claim 1 is characterized in that described video capture card is integrated in the computer of analytical system or is integrated in color video camera and the near-infrared video camera.
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| JP5930531B2 (en) * | 2012-05-24 | 2016-06-08 | 三菱電機エンジニアリング株式会社 | Imaging apparatus and imaging method |
| WO2014044220A1 (en) * | 2012-09-21 | 2014-03-27 | Wang Hao | Measurement mode selection device and measurement mode selection method |
| CN102871651A (en) * | 2012-10-26 | 2013-01-16 | 哈尔滨海鸿基业科技发展有限公司 | Near infrared lymphatic detector |
| WO2017117710A1 (en) * | 2016-01-05 | 2017-07-13 | 秀传医疗社团法人秀传纪念医院 | Imaging system and method for endoscopy |
| CN105962891B (en) * | 2016-04-21 | 2018-07-24 | 莱博泰克（大连）科技有限公司 | A kind of contact excitation light source of fluorescence imaging detecting system |
| CN106236265B (en) * | 2016-08-31 | 2018-08-21 | 北京数字精准医疗科技有限公司 | A kind of hand-held molecular image navigation apparatus and system |
| CN106943193A (en) * | 2017-04-27 | 2017-07-14 | 华中科技大学 | Common location operation guiding system and camera head |
| TWI667994B (en) * | 2017-12-29 | 2019-08-11 | 高雄榮民總醫院 | Imaging device and displaying method by using indocyanine green |
| CN108209878A (en) * | 2018-03-06 | 2018-06-29 | 南京生命源医药实业有限公司 | Visualization system and method |
| WO2019225693A1 (en) * | 2018-05-24 | 2019-11-28 | 学校法人 慶應義塾 | Surgical observation system |
| CN109171661A (en) * | 2018-10-10 | 2019-01-11 | 南京诺源医疗器械有限公司 | Fluoroscopic visualization checks system excitation light source |
| CN109363768A (en) * | 2018-10-10 | 2019-02-22 | 南京诺源医疗器械有限公司 | 785nm wavelength light source near-infrared fluorescence imaging surgery guides system |
| CN111887826A (en) * | 2020-08-10 | 2020-11-06 | 嘉兴市第二医院 | Fluorescent camera based on indocyanine green and application thereof |
| CN112235595A (en) * | 2020-11-04 | 2021-01-15 | 无锡蓝软智能医疗科技有限公司 | Operation scene dynamic presenting method, system and medium based on mixed reality technology |
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