CN102311980A - Method for preparing natural gardenia blue pigment - Google Patents

Abstract

The invention discloses a method for preparing natural gardenia blue pigment. The method comprises the steps of preparation of bacterial suspension, preparation of a solid medium, solid fermentation of microbes, preparation of gardenia blue, refining of a reaction solution with a macroporous adsorption resin, condensation of the refined solution, disinfection, spray drying, etc. After the above-mentioned steps are finished, mazarine solid gardenia blue pigment powder is eventually obtained. The technology of solid fermentation is employed in the invention; the invention has the advantages of a simple production process, convenient operation, little investment, low cost and high yield, and the color value of the product prepared by the method is higher than 160, so the invention has a good application prospect.

Description

A kind of method for preparing the natural gardenia cyanine

Technical field

The invention belongs to natural pigment preparation technique field, specifically relating to a kind of is substrate with the jasminoidin, the method for utilizing the microbial solid fermentation technology to produce the natural gardenia cyanine.

Background technology

Rubiaceae (Rubiaceae) plant cape jasmine (Gardenia jasminoides Ellis) is a traditional Chinese medicine; First medicine-food two-purpose resource that belongs to Ministry of Health's promulgation; Effect with purging intense heat relieving restlessness, clearing away heat and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body; Be used for that vexed, the yellow subcutaneous ulcer of pyreticosis urine is red, blood strangury and dry pain, blood-head tell nosebleed, conjunctival congestion with pain and swelling of the eye, pathogenic fire,toxin and furuncles, the sprain and contusion pain is controlled in external application.Cape jasmine distributes extensively in China, resource is very abundant, mainly is distributed in ground such as Jiangsu, Anhui, Jiangxi, Guangdong, Guangxi, Yunnan, Henan, Guizhou, Sichuan, Hubei, Fujian, Taiwan.Cape jasmine mainly contains compositions such as iridoids, style and stigma of Saffron Crocus acids, organic acid ester, flavonoid, has cholagogic, anti-inflammatory, analgesia, step-down, effect such as antiviral.

Along with the development of China's foodstuffs industry and the raising of living standards of the people, also increasingly high to the health requirements of self, so food-safety problem also progressively becomes focus.Pigment is used very extensive in food-processing, and some synthetic food colors are because safety-problems is controversial, and many in the world countries ban use of some synthetic food color.Therefore, the natural pigment of development and utilization safety non-toxic receives global attention day by day.That mostly natural pigment is is red, yellow two types of colors, and in red, yellow, primary colors, natural blue pigment is but very rare, lacked cyanine and just allocate not go out green, purple, color such as brown, also with regard to satisfied not the color matching on the numerous food product, painted needs.

In the prior art; The microorganism culturing fermentation is main in the production of natural gardenia cyanine adopts the liquid submerged fermentation method, is higher than 140 product but this method is difficult to produce outstanding valency .For this reason, a kind of working method that can access high luminance relay valency natural gardenia cyanine and be convenient to suitability for industrialized production of research and development just becomes the technical problem that needs to be resolved hurrily in the prior art.

Summary of the invention

The objective of the invention is to deficiency, a kind of new natural gardenia cyanine preparation method is provided, adopt the microbial solid fermentation technology and combine macroporous adsorbent resin refining, to realize the large-scale production of high luminance relay valency natural gardenia cyanine to prior art.

The object of the invention is achieved through following technical proposals.

Except as otherwise noted, the percentage ratio that is adopted among the present invention is mass percent.

A kind of method for preparing the natural gardenia cyanine may further comprise the steps:

(1) preparation of bacteria suspension: the aspergillus oryzae bacterial classification is cultivated based on 28～33 ℃ of following activation with PDA, and is prepared into bacteria suspension;

(2) preparation of solid medium: add nutritive medium in the wheat bran with moistening wheat bran, the nutritive medium consumption is 1.5～2.0 times of wheat bran weight, and the back that stirs obtains required solid medium in 121 ℃ of 20min that sterilize down after the cooling, subsequent use; The proportion of composing of described nutritive medium is: 2.0～3.0% (NH ₄) ₂SO ₄, 2.8～3.8%KH ₂PO ₄, 0.008～0.010%MgSO ₄.7H ₂O, 0.008～0.010%ZnSO ₄.7H ₂O and 0.018～0.020%CaCl ₂, all the other are water;

(3) microbial solid fermentation: in the solid medium for preparing, add 4～5% step (1) gained bacteria suspension, fermentation culture 96～108 hours, culture temperature are 28～33 ℃;

(4) preparation of gardenia blue: it is 3.0～6.0% the aqueous solution that jasminoidin is mixed with content, and is heated to 46 ℃, adds 0.6～1.2% step (3) gained solid fermentation product; Under 100～180r/min stirring velocity, add monosodium glutamate after 6 hours in 40～50 ℃ of reactions; Addition is 50～70% of a jasminoidin, continues reaction and is warming up to 80～90 ℃ after 40～46 hours, is incubated 2 hours; Be cooled to room temperature then, filter;

(5) refining, dry: step (4) gained filtrating is made with extra care with macroporous adsorbent resin earlier; Refined liquid concentrates, and 90 ℃ of insulations 30 minutes down, promptly obtains required natural gardenia cyanine after spray-dried again; 160～190 ℃ of spray-dired inlet temperatures, 65～80 ℃ of temperature outs.

Wherein, the described macroporous adsorbent resin of step (5) is the DM-2 of the anti-upright section in Shandong, blue LSA-21, the LSA-40 that knows in XDA16, XDA16N or Xi'an of ROHM AND HAAS.

The present invention is a substrate with the jasminoidin, utilizes solid-fermented technique, and adopts macroporous adsorbent resin refining; Blue its production technique of plain color valency

of the natural gardenia for preparing is simple; Easy to operate, to invest for a short time, cost is low; Yield is high, has a good application prospect.

Description of drawings

Fig. 1 is preparing method's of the present invention process flow sheet

Embodiment

Below in conjunction with accompanying drawing and embodiment the present invention is done further detailed description.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in qualification the present invention.

Embodiment 1

Preparation natural gardenia cyanine, Production Flow Chart is as shown in Figure 1.

(1) preparation of bacterial classification and bacteria suspension

Aspergillus oryzae bacterial classification (available from the Yunnan Prov. Inst. of Microbiology, numbering ym3051) is inoculated on the PDA substratum, cultivates down, treated to deposit in 0～5 ℃ of refrigerator after a large amount of brown spores generate and preserved in 29～30 ℃.Process bacteria suspension with the SPSS bacterial strain that activation is good before using, making its concentration is 10 ⁷～10 ⁸Cfu/mL.

(2) solid fermentation medium preparation

30g wheat bran is placed in the petridish of 150mm, add the 45mL nutritive medium, in 121 ℃ of 20min that sterilize down, be cooled to about 30 ℃ behind the mixing, subsequent use.

Nutrient solution prescription: 2.3% (NH ₄) ₂SO ₄, 3.0%KH ₂PO ₄, 0.010%MgSO ₄.7H ₂O, 0.010%ZnSO ₄.7H ₂O, 0.020%CaCl ₂, all the other are water.

(3) microbial solid fermentation

Solid medium inserts bacteria suspension 3.0mL in Bechtop, stir with the glass stick of sterilizing in advance, in 30～31 ℃ of constant incubators, cultivates 96 hours.

(4) preparation of gardenia blue

With content is 40% jasminoidin 300g, is mixed with 3000g solution, and average mark is loaded in 20 250mL Erlenmeyer flasks, is heated to 46 ℃; In each bottle, add the solid fermentation product in the 1.5g step (3) respectively, place in the constant temperature shaking table, reaction is after 6 hours under 45 ℃, 125～130r/min, and each adds the 3.0g monosodium glutamate; Continue reaction 42 hours, reaction solution is heated to 90 ℃ with water-bath, and is incubated 2 hours; Be cooled to room temperature then, filter, filtrating merges.

(5) gardenia blue is refining

Filtrating is made with extra care with macroporous adsorbent resin LSA-21.

(6) spraying drying

Refined liquid concentrates, and carries out spraying drying in insulation under 90 ℃ after 30 minutes and obtains 80.1g mazarine pressed powder (160～190 ℃ of spray-dired inlet temperatures, 65～80 ℃ of temperature outs).

Embodiment 2

(1) preparation of bacterial classification and bacteria suspension

The aspergillus oryzae bacterial classification is inoculated on the PDA substratum, cultivates down, treated to deposit in 0～5 ℃ of refrigerator after a large amount of brown spores generate and preserved in 31～32 ℃.Process bacteria suspension with the SPSS bacterial strain that activation is good before using, making its concentration is 10 ⁷～10 ⁸Cfu/mL.

(2) preparation of solid medium

In 1kg wheat bran, add the 2kg nutritive medium and stir, make it wetting, then will wetting wheat bran pack in the Stainless Steel Disc, the about 1.5cm of bed thickness in the 121 ℃ times 20min that sterilize, is cooled to about 30 ℃, and is subsequent use.

Nutrient solution prescription: 2.0% (NH ₄) ₂SO ₄, 2.8%KH ₂PO ₄, 0.008%MgSO ₄.7H ₂O, 0.008%ZnSO ₄.7H ₂O, 0.018%CaCl ₂, all the other are water.

(3) microbial solid fermentation

Solid culture stirs with the glass stick of sterilizing in advance based on inserting bacteria suspension 150mL in the Bechtop, in 30～31 ℃ of constant incubators, cultivates 108 hours.

(4) preparation of gardenia blue

In the fermentor tank of 300L, be 40% jasminoidin 20kg with content, be mixed with 200kg solution; Be heated to 46 ℃, add the solid fermentation product in the 1.6kg step (3), in 42～45 ℃, 175～180r/min reaction after 6 hours; The monosodium glutamate that adds 4kg continues 45 hours post-heating to 80 of reaction ℃, and is incubated 2 hours; Be cooled to room temperature then, filter.

(5) gardenia blue is refining

Filtrating is made with extra care with macroporous adsorbent resin DM-2.

(6) spraying drying

Refined liquid concentrates, and carries out spraying drying in insulation under 90 ℃ after 30 minutes and obtains 6.1kg mazarine pressed powder (160～190 ℃ of the inlet temperatures of dusting, 65～80 ℃ of temperature outs).

Embodiment 1,2 gained gardenia blue pigment products are carried out physics and chemistry detect, its result sees table 1.

The physics and chemistry and the microbiological indicator of table 1 gardenia blue pigment product

Can know by table 1; The gardenia blue pigment product that adopts patent art to produce; Its look valency

all is not less than 160, and obviously the look valency than the gardenia blue pigment that has solution fermentation production now is high; Other physics and chemistry and microbiological indicator also reach the relevant requirement of foodstuff additive.

The above is merely preferred embodiment of the present invention, not in order to the restriction the present invention, all any modifications of within spirit of the present invention and principle, being done, be equal to the replacement and the improvement, all should be included within protection scope of the present invention.

Claims (2)

1. method for preparing the natural gardenia cyanine may further comprise the steps:

(1) preparation of bacteria suspension: the aspergillus oryzae bacterial classification is cultivated based on 28～33 ℃ of following activation with PDA, and is prepared into bacteria suspension;

(2) preparation of solid medium: add nutritive medium in the wheat bran with moistening wheat bran, the nutritive medium consumption is 1.5～2.0 times of wheat bran weight, and the back that stirs obtains required solid medium in 121 ℃ of 20min that sterilize down after the cooling, subsequent use; The proportion of composing of described nutritive medium is: 2.0～3.0% (NH ₄) ₂SO ₄, 2.8～3.8%KH ₂PO ₄, 0.008～0.010%MgSO ₄.7H ₂O, 0.008～0.010%ZnSO ₄.7H ₂O and 0.018～0.020%CaCl ₂, all the other are water;

(3) microbial solid fermentation: in the solid medium for preparing, add 4～5% step (1) gained bacteria suspension, fermentation culture 96～108 hours, culture temperature are 28～33 ℃;

(4) preparation of gardenia blue: it is 3.0～6.0% the aqueous solution that jasminoidin is mixed with content, and is heated to 46 ℃, adds 0.6～1.2% step (3) gained solid fermentation product; Under 100～180r/min stirring velocity, add monosodium glutamate after 6 hours in 40～50 ℃ of reactions; Addition is 50～70% of a jasminoidin, continues reaction and is warming up to 80～90 ℃ after 40～46 hours, is incubated 2 hours; Be cooled to room temperature then, filter;

(5) refining, dry: step (4) gained filtrating is made with extra care with macroporous adsorbent resin earlier; Refined liquid concentrates, and 90 ℃ of insulations 30 minutes down, promptly obtains required natural gardenia cyanine after spray-dried again; 160～190 ℃ of spray-dired inlet temperatures, 65～80 ℃ of temperature outs.

2. the method for preparing the natural gardenia cyanine according to claim 1 is characterized in that: the described macroporous adsorbent resin of step (5) is the DM-2 of the anti-upright section in Shandong, blue LSA-21, the LSA-40 that knows in XDA16, XDA16N or Xi'an of ROHM AND HAAS.

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