CN102304470B - Multichannel automatic bacteria rapid inoculator and using method thereof - Google Patents

Abstract

The invention belongs to the technical field of medical test, and in particular relates to a multichannel automatic bacteria rapid inoculator and a using method thereof. Compared with all the existing automatic bacteria inoculators, a complete innovated inoculation principle is adopted for the invention, thus the bacteria inoculation is more simple, convenient and rapid; specimens can be unfolded in layers on a panel, the separating degree of the specimens on the panel is improved, single bacterial colony can be obtained sufficiently; simultaneously the multiple inoculation channel design improves working efficiency greatly, and ten specimens can be treated well within five minutes easily (30 panels are inoculated); and the inoculator is clean and is not polluted, and corresponds to the requirements of biosafety. Compared with the similar inoculators, the inoculator provided by the invention has the characteristics of high working efficiency and low inoculator production cost, is convenient to operate, and is suitable for popularization and using in each level of microbiological labs.

Description

A kind of Multichannel automatic bacteria rapid inoculator and using method thereof

Technical field

The invention belongs to medical test technical field, be specifically related to a kind of development and using method thereof of Multichannel automatic bacteria rapid inoculator.

Background technology

Bacterium separation and Culture is the basis of carrying out Bacteria Identification and drug sensitivity test.Utilizing transfering loop sectional streak to obtain single bacterium colony, is classical microbionation method.Owing to the working conditions such as instrument, equipment being required to promote compared with low being convenient to, the method is used till today always.But also there are some drawbacks in the method.First, the method is had relatively high expectations to individual operant level, must accomplish aseptic technique, by strict training and prolonged operation, puts into practice, and could obtain desired separated effect; Secondly, in the process of inoculation sample, need to light the naked lights such as spirit lamp, electric furnace, in order to transfering loop quick sterilization, and use naked light to exert an influence to the air-flow of Biohazard Safety Equipment, and when processing volatile one and inflammable articles, also easily cause danger; Again, Inoculation Method, overall efficiency is low, and affected by operator's individual's level and inoculation custom, is difficult to carry out omnidistance quality control, does not meet laboratory to automatization, standardized requirement; In addition, in seeded process, sample is exposed in air completely, operator and sample close contact, and self health is on the hazard, and does not meet the requirement that laboratory Biosafety is more and more higher.

The automatization of microbionation and stdn are one of main directions of Clinical microorganism laboratory study and development.At present, Clinical microbiology experiment chamber is seldom equipped with automation bacteria inoculation instrument.In the world, except a small amount of large-scale experiment chamber is equipped with automatic bacterial separation and Culture system, only have a few automation bacteria inoculation instrument to put on market, and majority is still in the gradual perfection stage.With regard to practical application, automation bacteria inoculation instrument be far from Biochemical Analyzer or blood-counter system ripe.Wherein, the most representative with the easySpiral Pro full automatic screw inoculation instrument of French interscience company development.This instrument has been released at least three generation products at present, has successfully realized the automation processes such as automatic sample, automatic vaccination, automatic disinfection, once inoculates whole process only tens of seconds.

Yet also there are some open defects in easySpiral Pro full automatic screw inoculation instrument.As, in seeded process, the plate opening upwards of opening is completely exposed in air, is easy to be subject to bacterial contamination in ambient air, can only in Biohazard Safety Equipment, operate; Secondly, higher to dull and stereotyped appearance requirement, must use dressing plate, self-control substratum possibly cannot be inoculated.The thickness of agar, planeness, smooth surface degree in culture dish, and have or not cavity etc., all very large on the impact of effect of inoculation, as, the flat board in inoculating needle contact high speed rotating, may roll slightly irregular agar in flat board, makes inoculation failure; Again, what in whole seeded process, use is same inoculating needle, although adopted new technology, and all clean, sterilize after each inoculation, but, because offseting toxic agent, part bacterium there is very strong resistibility, especially can form brood cell's bacterium, within very short time, thoroughly kill all bacteriums, be almost impossible; Simultaneously, feature due to sample self, when processing containing toughness samples such as the larger ight soil of miscellaneous bacteria amount, sputums, by Rapid Cleaning, sterilization, be difficult to stop completely the generation of crossed contamination, in addition, inoculating needle also likely carries remaining sterilizing agent, and the bacterial growth in follow-up sample is produced and suppressed.

Easy and simple to handle, working efficiency height is the outstanding advantages of self-reacting device.Although seeded process of easySpiral Pro full automatic screw inoculation instrument only tens of seconds, this only refers to the time that inoculation needs, bacterium sample is different from biochemistry, immune sample, most of sample will carry out pre-treatment, as phlegm, and because artificial replacing when dull and stereotyped needs to pause, in fact the process of a plurality of sample operate continuouslys is difficult to realize continuous full speed and carries out.In addition, this instrument is single armed (single passage) inoculation instrument, can only process a sample at every turn, and each working cycle (comprise cleaning and sterilizing, inhale sample, seeded process) also can only complete 1 plating, 1 part of sample may be inoculated 3 different flat boards, needs artificial 3 flat boards of changing.If process 100 parts of samples every day, will suspend and manually change dull and stereotyped 300 times, wherein every part of sample all requires to change not identical flat board 3 times, not only have a strong impact on the working efficiency of instrument, and, require to change correctly, fast and continually different flat boards, can increase staff's labour intensity.Although realized the automation processes such as automatic sample, automatic vaccination, automatic disinfection, in actual mechanical process, still require at least 1 staff to keep close and assist, the advantage of automatization cannot be given full play of.

Object of the present invention is a kind of Multichannel automatic bacteria rapid inoculator of design and introduce its using method.Different from existing all automation bacteria inoculation instrument, the present invention adopts the inoculation principle of innovation completely, makes microbionation more easy, quick.Be similar to bernoulli principle, high pressure gas are pressed into liquid sample after the tubule in special specimen cup, from micropore ejection, run into outside air and be atomized, and it is upper to be sprayed directly into flat board, thereby reach the object of inoculation.Specimen amount by the flat board in water smoke direct projection region (dull and stereotyped central zone) inoculation is large, and few away from the region inoculation specimen amount of direct projection, only vaporific inoculation, thus realize expansion that sample is had levels on flat board, improved the dispersity of sample on flat board.Appropriate sample has been inoculated in central zone, guarantees detection sensitivity; And dull and stereotyped peripheral region adopts vaporific dispersion, can obtain enough single bacterium colonies.Meanwhile, a plurality of inoculation channels designs, have improved working efficiency greatly, and 1 10 parts of sample (inoculate 3 kinds, totally 30 flat boards) can easily be handled well, and avoid the frequent dull and stereotyped trouble (only changing flat board 3 times) of bringing of changing in 5 minutes; Using cleaning compressed air as push agent, clean, nuisanceless, meet the requirement of Biosafety.And with existing same quasi-instrument comparison, that the present invention has is easy to operate, working efficiency is high, instrument low production cost, be adapted at the feature that each level Microbiological Lab promotes, uses.

Summary of the invention

The hyperchannel quick bacteria inoculation instrument of high pressure gas as motivating force usingd in the present invention.Host machine part mainly comprises special sample cup, sample cup bracket, plate bracket, plate aseptic cover and five parts of gas distributing system, and whole instrument outside is with stainless steel casing closure; Wherein be fixed on plate bracket on main frame directly over sample cup bracket, and make each plate of placing on plate bracket can be corresponding one by one with the sample cup on sample cup bracket; Every main frame is furnished with each 2 pairs, sample cup bracket and plate bracket, independently inoculates passage for 10, once can process 10 parts of samples.In addition, every main frame is furnished with 1,1 of small-sized electric exhaust fan (can match high-quality host computer radiating electric fan), 1 of miniature electric air lift pump and gaseous tension variable valve, and is built in main frame.In hundred grades of laminar flow hood of air lift pump inlet mouth configuration, to purify air; The pressurized air that air lift pump produces, through gas regulation valve regulation pressure, makes pressure maintain 0.25MPa; Pressurized gas is shunted by gas distributing system, the sample distributed power during as inoculation; Exhaust fan can dispel the heat and derive issuable waste gas in main frame by assist gas pressure pump, the discharge direction of guiding gas, and derive with rubber hose, Biohazard Safety Equipment waste gas outlet can be led in exit.

Composition and the design of first part's instrument major parts.

The design of special sample cup.Sample cup of the present invention comprises a tetrahedron cup of being made by PVC (long 40mm, wide 40mm, high 50mm); Groove at a diameter 8mm of tetrahedron cup one lateral extent rim of a cup upper limb 10mm place design, groove central authorities open a diameter 5mm aperture, for being connected and mating with gas service pipes end, aperture opening part communicates with in cup by conduit, and catheter tip approaches sample liquid level; On sample cup, there are lid and cup by helixseal, the fine duct that design diameter in lid center is 2.5mm leads in cup, conduit upper end is connected with lid and seals, lid is that 0.2mm aperture communicates with the external world over against 1 diameter of centre design of conduit, aperture place is a little less than plate lid 1mm left and right (Fig. 1), aperture place seals with aseptic waterproof self-adhesive paper, during inoculation sample, unties.

The design of sample cup bracket.Sample cup bracket is rectangular parallelepiped light aluminum alloy (long 950mm, wide 60mm, high 60mm) can, on its end face, design successively five rectangular parallelepiped grooves (long 40mm, wide 40mm, high 50mm), mate with sample cup shape, size, the 3rd groove is positioned at bracket central authorities, all the other each groove central points are successively at a distance of 170mm(Fig. 2), thus the position of fixed sample cup; On bracket, the same side at a five groove positions diameter of outside design is 10mm aperture, for gas service pipes end, passes and is connected with sample cup side aperture.The outside both wings of sample cup bracket bottom surface are designed with 2 groups of litter, respectively with main frame on the track of the sample cup bracket fixed position that designs match, the bracket of design can pull out and advance from main frame glibly like this, and, after being advanced main frame, its position is fixed, thereby the position of each sample cup in main frame also fixed.

The design of plating bracket.Plating bracket is rectangular parallelepiped light aluminum alloy (long 950mm, wide 180mm, high 90mm) can, on its end face, design successively five circular flat bottom grooves (dark 30mm), interior diameter and be divided into two kinds of 150mm and 90mm, being that 150mm and 90mm are dull and stereotyped with diameter mates; Groove central authorities sink, and forming respectively diameter is 140mm (corresponding to 150mm groove) and 80mm (corresponding to 90mm groove) U-shaped groove, bottom opening, and diameter is 5mm; Flat bottomed recess has 1 circle to lower recess along circumference, and width is about 2mm, degree of depth 5mm (size is mated with plate); The 3rd groove is positioned at bracket central authorities, and all the other each groove central points are successively at a distance of 170mm, thus the position of fixed flat planar; The outside both wings of plating bracket bottom surface are designed with 2 groups of litter, respectively with main frame on the track of the plating bracket fixed position that designs match, plating bracket can pull out and advance from main frame glibly like this, and, after being advanced main frame, its position is fixed, thereby the position of each groove (flat board) in main frame also fixed, and corresponding with the position of each sample cup, and to make the 0.2mm opening on sample cup bowl cover be the central authorities (Fig. 3,4) of 5mm opening over against U-shaped recess base diameter.

The design of plate aseptic cover.Adopt plastics film press mold, the groove on designed size, shape and plating bracket matches, the opening of U-shaped bottom portion of groove, diameter is 5mm(Fig. 4), disposable use after ethane via epoxyethane sterilization.On plating bracket, the flat bottomed recess use of connecting with U-shaped groove, covers plate aseptic cover above, and dull and stereotyped left-hand thread in flat bottomed recess, forms " secret room " on plating bracket.Like this, when specimen inoculation, plate aseptic cover is in order to protect plating bracket not contaminated; Before inoculation next time, the aseptic cover of again changing can be isolated flat board and plating bracket, and protection flat board is not contaminated, solves the cross-contamination issue that may exist between sample.

The design of gas distributing system.With interior diameter 10mm high-quality plastics pipe, design the closed tube of 2 groups of mutual UNICOMs, every five 1 group, in the one end being connected with sample cup, be connected a segment length 100mm, interior diameter 5mm stainless steel metal pipe, metal tube end connecting length is about 5mm, has elastic rubber hose, rubber tubing outside diameter is 7mm, diameter of bore is 5mm, mates with the connecting portion on sample cup; 5 metal communicating pipe are one group (Fig. 5), are fixed on 1 pull bar in main frame, and closed tube terminal position is corresponding with each sample cup on sample cup bracket, and whole pull bar can move forward and backward, and can not move left and right.The control principle that adopts similar ballpoint pen nib to move up and down, when pressing " UNICOM/break key " (control pull bar), spring ejects, to before metal tube end, push away, make its rubber hose part tightly be pressed in the connecting portion on sample cup, rubber has plasticity-, the two is closely connected and air tight; Again press " UNICOM/break key ", spring is packed up, and the two separation is convenient to sample cup bracket and is moved freely; 2 groups of closed tubes are controlled through same gas distribution manifold, and house steward is controlled by the rubber piston in a middle tool hole on host panel.While pressing " inoculation " key, piston tool bore portion enters gas distribution manifold, and gas passage is connected; Decontrol " inoculation " key, spring is upspring piston, and piston tool bore portion leaves gas distribution manifold, and gas passage is by piston closes, and gas is closed.

Accompanying drawing explanation

Accompanying drawing provides composition and the structural representation of hyperchannel quick bacteria inoculation instrument major parts of the present invention

The side-looking two dimensional structure schematic diagram of Fig. 1 special sample cup of the present invention

The structural representation of Fig. 2 sample cup bracket of the present invention

Fig. 3 plating bracket structural representation of the present invention

Fig. 4 plate aseptic cover of the present invention structural representation

Fig. 5 gas distributing system structural representation of the present invention

Fig. 6 hyperchannel quick bacteria inoculation of the present invention instrument principle of work schematic diagram.

Second section, hyperchannel bacterium inoculates instrument using method fast.

Instrument is prepared.Open host power supply, open electric exhaust fan, air lift pump, and regulate pressure to 0.25MPa.

Inoculation.By aseptic collection in the sample number of sample cup, untie the aseptic waterproof self-adhesive paper in sample cup aperture place; 2. sample cup bracket is pulled out from main frame, put in order sample cup to corresponding position pusher and enter main frame; 3. plating bracket is pulled out from main frame, dull and stereotyped groove adds after 1 plate aseptic cover, opens wish and inoculates dull and stereotyped left-hand thread and push main frame at corresponding dull and stereotyped groove tail-bracket; 4.An“ UNICOM/break key " 1 time; 5. press " inoculation " key 1 time; 6.An“ UNICOM/break key " 1 time; 7. pull out plating bracket, take out and inoculated flat board, after adding a cover, put into incubator and cultivate.With a sample, inoculate other flat boards as need, as long as put corresponding flat board well and push main frame, An“ UNICOM/break key again " and " inoculation " key.

Advantage of the present invention.The special specimen cup of this instrument selection formed objects and bore, adding equal-volume sample, adopt constant air pressure, is quantitative at the liquid of same time ejection, thereby can realize sample, quantitatively inoculate, and more directly, more accurate; , by developing the specimen cup of different sizes and bore, and air pressure is regulated meanwhile, can change easily specimen inoculation amount, to meet the individual requirements such as different specimens type and different vaccination amount; Except the samples such as sputum, ight soil need pre-treatment, most liquid samples are (as urine, cerebrospinal fluid, hydrothorax, ascites etc.) not only can directly be collected in specimen cup, and this sample cup can be directly used in inoculation sample, crossed contamination between the sample of having avoided may causing in sample application of sample, transfer process.Abandon the inoculating needle (transfering loop) of continuing to use a century, the dispersion system of gas as power usingd in employing, success realizes the effective separation to bacterium in sample, this is the design of a complete novelty, simultaneously, avoided in Biohazard Safety Equipment, using naked light, stopped because inoculating needle carries crossed contamination between the sample that may cause, the sterilizing agent of having stopped because bringing with sterilizing agent sterilization, processing inoculating needle (transfering loop) is remaining; Another advantage of abandoning inoculating needle (transfering loop) is, dull and stereotyped appearance requirement is reduced greatly, agar thickness, planeness, smooth surface degree, and have or not cavity, on inoculation almost without any impact; The introduction of the design of U-shaped groove on plating bracket, plate aseptic cover, and treat dull and stereotyped Open Side Down " secret room " effect that left-hand thread forms of inoculation, solved the problem that may have pollution between sample; Using pressurized air as push agent, clean, nuisanceless, meet the requirement of Biosafety; The most important thing is, inoculate channels designs more, greatly improved working efficiency, within 5 minutes, can easily process 10 parts of samples, inoculate 30 flat boards, avoided the frequent dull and stereotyped trouble of bringing of changing.With existing same quasi-instrument comparison, high, the whole instrumentation of working efficiency of the present invention is with low cost, and instrument when work is very low to outside conditional request, except can be the application of large-scale experiment chamber, also be adapted at Microbiological Lab of basic unit and promote the use of, there are wide market outlook.

Claims (2)

1. a Multichannel automatic bacteria rapid inoculator, its host machine part primary structure comprises sample cup, sample cup bracket, plating bracket, plate aseptic cover and five parts of gas distributing system, it is characterized in that: every main frame is furnished with 10 and independently inoculates passage, once can process 10 increments originally; On tetrahedron sample cup cup body and sample cup bracket, the design of rectangular parallelepiped groove compatibility, for the position of fixed sample cup, avoids cup to move on bracket; Sample cup side is provided with conduit, for gas service pipes terminal matching; Sample cup bracket and plating bracket adopt organic matched-pair design, for hyperchannel inoculation, connect; Plating bracket is provided with flat bottomed recess and U-shaped groove, and both uses of connecting, cover plate aseptic cover above, and dull and stereotyped left-hand thread in flat bottomed recess, forms " secret room " on plating bracket; On sample cup bracket and plating bracket, design litter, and match with the track on main frame, make the two can pass in and out flexibly main frame; In seeded process, using laminar flow hood filtered air as power.

2. a kind of Multichannel automatic bacteria rapid inoculator according to claim 1, is characterized in that, inoculation instrument is furnished with automatization sample injector and/or automatic flat-plate transmission system simultaneously.

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