CN102282928A - Artificial method for physical dormancy release of Zunghar leaf-free bean seeds - Google Patents
Artificial method for physical dormancy release of Zunghar leaf-free bean seeds Download PDFInfo
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- CN102282928A CN102282928A CN2011101588360A CN201110158836A CN102282928A CN 102282928 A CN102282928 A CN 102282928A CN 2011101588360 A CN2011101588360 A CN 2011101588360A CN 201110158836 A CN201110158836 A CN 201110158836A CN 102282928 A CN102282928 A CN 102282928A
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Abstract
The invention relates to an artificial method for physical dormancy release of rare sand dune plant Zunghar leaf-free bean seeds. The method comprises the following steps of: soaking fresh and mature Zunghar leaf-free bean seeds into hot water or concentrated sulfuric acid, checking the imbibition rate and the water absorption curve of the seeds by a conventional method, observing the imbibition process and the histological change of the treated seeds in real time by using a scanning electronic microscope, and finally determining the weakest dormancy release part, namely the 'water gap' part. The concentrated sulfuric acid treatment method can efficiently and quickly break physical dormancy of the seeds, has the characteristics of time and labor saving, simplicity and convenience for operation and high efficiency, and can break the natural population update limiting bottleneck, namely low seed germination rate. The determined weakest dormancy release part of the seeds is hilum (namely 'water gap'); and the hilum is cracked, then the caruncle is broken, and a large amount of moisture enters the seeds, that the seeds are imbibed, hard husks are broken, and the physical dormancy release is realized. An economic, practical, simple and convenient method is provided for artificial recovery of wild Zunghar leaf-free bean populations.
Description
Technical field
The present invention relates to a kind of sand dune rare plant Zhunger Basin that is used for and do not have the manual method that the dormancy of leaf beans seed physical property discharges.
Background technology
Sand dune rare plant Zhunger Basin does not have the rational dormancy phenomenon of leaf beans seed existence, and its open-air population seedling is few, and rare seedling builds up, and the dormancy of seed physical property is to hinder the important step that population upgrades.Upgrade in order effectively to finish the manual reversion population, it is crucial abolishing its seed physical property dormancy.Yet at present, do not have the best manual method of leaf beans seed physical property dormancy, there is no fixing, effective ways about abolishing Zhunger Basin; And " mouth of a river " is that the weakest position is abolished in the dormancy of seed physical property, and the cracking at the mouth of a river and seed imbibition subsequently are the signs of physical property dormancy breaking.Position, this kind mouth of a river and histology thereof define does not still have any report but at present.The invention reside in screening and abolish the best approach that Zhunger Basin does not have the dormancy of leaf beans seed physical property, and define weak part-" mouth of a river " of abolishing the dormancy of seed physical property.
Summary of the invention
The objective of the invention is, provide a kind of sand dune rare plant Zhunger Basin that is used for not have the manual method that the dormancy of leaf beans seed physical property discharges, this method does not have leaf beans seed with the Zhunger Basin of fresh maturation, carry out immersion treatment with the hot water or the concentrated sulfuric acid, check the imbibition rate and the water suction curves of seed then according to a conventional method, and utilize ESEM Real Time Observation seed imbibition process and histology thereof after treatment to change, determine that finally dormancy abolishes weak part, the i.e. position at " mouth of a river ".This method can be broken the dormancy of seed physical property efficiently, fast, has the characteristics time saving and energy saving, easy and simple to handle, that efficient is high, and the restriction bottleneck-seed germination rate that can break the renewal of nature population is low.It is that Zhunger Basin does not have in the leaf beans seed imbibition process weak part (i.e. " mouth of a river ") that the present invention defines hilum, and hilum at first ftractures, and strophiole breaks subsequently, and moisture enters seed in a large number and finishes the imbibition process, realizes the release of physical property dormancy.
Zhunger Basin of the present invention does not have the manual method that the dormancy of leaf beans seed physical property discharges, and follows these steps to carry out:
A, the Zhunger Basin of fresh maturation is not had leaf beans seed, carry out immersion treatment, check the imbibition rate and the water suction curves of seed then according to a conventional method with the hot water or the concentrated sulfuric acid, wherein hot water temperature 90-100 ℃, soak time is 15-20min, and concentrated sulfuric acid concentration is 98%, soak time 28-32min;
B, the seed after step a soaked utilize seed imbibition process and histology thereof after the ESEM Real Time Observation hot water and the concentrated sulfuric acid are handled to change, and determine that finally dormancy abolishes weak part, the i.e. position at " mouth of a river ".
The imbibition rate of step a seed is respectively hot water 27.11 ± 5.4%, the concentrated sulfuric acid 100%.
Zhunger Basin of the present invention does not have the manual method that the dormancy of leaf beans seed physical property discharges, this method is by selecting running water, hot water, sodium hydroxide solution, manually scratch (using sand papering) until kind of a skin breakage, processing such as the concentrated sulfuric acid, the imbibition rate and the water suction curves of check seed, its imbibition rate is respectively 3.3 ± 1.2%, 27.11 ± 5.4%, 3.41 ± 1.0%, 100%, 100%, water suction curves shows, as time passes, manually scratch the seed imbibition curve of handling with the concentrated sulfuric acid and raise the soonest, the seed imbibition curve of hot water treatment is placed in the middle, running water and sodium hydroxide solution do not make significant difference to the seed imbibition, illustrate manually to scratch and thoroughly effectively to break the dormancy of seed physical property, but waste time and energy, handle so the best method of breaking the dormancy of seed physical property is the concentrated sulfuric acid because of manually scratching with the concentrated sulfuric acid.Utilize the ESEM Real Time Observation again, and define this kind seed dormancy dispose procedure weak part-" mouth of a river " from the histology angle and show: the hot water treatment and the concentrated sulfuric acid are handled and can be broken Zhunger Basin and do not have the dormancy of leaf beans seed physical property.Wherein, concentrated sulfuric acid processing method can be broken the dormancy of seed physical property efficiently, fast, has the characteristics time saving and energy saving, easy and simple to handle, that efficient is high, and the restriction bottleneck-seed germination rate that can break the renewal of nature population is low.It is that Zhunger Basin does not have in the leaf beans seed imbibition process weak part (i.e. " mouth of a river ") that the present invention defines hilum, and hilum at first ftractures, and strophiole breaks subsequently, and moisture enters seed in a large number and finishes the imbibition process, realizes the release of physical property dormancy.
Description of drawings
Fig. 1 is the imbibition figure of seed of the present invention, wherein
The people for scratch,
Running water,
The concentrated sulfuric acid,
Hot water,
Sodium hydroxide.
Fig. 2 is for after the concentrated sulfuric acid of the present invention handles, by the tissue morphology structural change figure of scanning electron microscopic observation the surface of the seed, wherein A: the whole seed of fresh maturation; B: the kind skin (300um) of fresh mature seed; C: kind skin (100um) D of fresh mature seed: the concentrated sulfuric acid is handled the hilum behind the 2min; E: the concentrated sulfuric acid is handled the strophiole behind the 2min; F: the concentrated sulfuric acid is handled the hilum behind the 5min; G: the concentrated sulfuric acid is handled the strophiole behind the 5min; H: the concentrated sulfuric acid is handled the hilum behind the 10min; I: the concentrated sulfuric acid is handled the strophiole of 10min; J: the concentrated sulfuric acid is handled the hilum of 15min; K: the concentrated sulfuric acid is handled the strophiole (300um) of 15min; L: the concentrated sulfuric acid is handled the strophiole (100um) of 15min; M: the concentrated sulfuric acid is handled the hilum of 20min; N: the concentrated sulfuric acid is handled the strophiole of 20min; O: the concentrated sulfuric acid is handled the whole seed of 30min; P: the concentrated sulfuric acid is handled the hilum (300um) of 30min; Q: the concentrated sulfuric acid is handled the hilum (100um) of 30min; R: the concentrated sulfuric acid is handled the kind skin of 30min
Fig. 3 is after hot water of the present invention (95 ℃) is handled, by the tissue morphology structural change figure of scanning electron microscopic observation the surface of the seed, the wherein hilum of A: hot water treatment 5min; The strophiole of B: hot water treatment 5min; The hilum of C: hot water treatment 10min; The strophiole of D: hot water treatment 10min; The hilum of E: hot water treatment 20min; The strophiole of F: hot water treatment 20min.
Embodiment
Further describe and provide embodiment below in conjunction with accompanying drawing
Embodiment 1
A, the Zhunger Basin of fresh maturation is not had leaf beans seed, carry out immersion treatment with hot water, check the imbibition rate and the water suction curves of seed then according to a conventional method, wherein the hot water temperature is 90 ℃, and soak time is 15min, and the imbibition rate of seed is 27.11 ± 5.4%;
B, the seed after step a soaked utilize seed imbibition process and histology thereof after the ESEM Real Time Observation hot water treatment to change, and determine that finally dormancy abolishes weak part, the i.e. position at " mouth of a river ".
Embodiment 2
A, the Zhunger Basin of fresh maturation is not had leaf beans seed, carry out immersion treatment with the concentrated sulfuric acid, check the imbibition rate and the water suction curves of seed then according to a conventional method, wherein concentrated sulfuric acid concentration is 98%, soak time 28min, and the imbibition rate of seed is the concentrated sulfuric acid 100%;
B, the seed after step a soaked utilize seed imbibition process and histology thereof after the ESEM Real Time Observation concentrated sulfuric acid is handled to change, and determine that finally dormancy abolishes weak part, the i.e. position at " mouth of a river ".
Embodiment 3
A, the Zhunger Basin of fresh maturation is not had leaf beans seed, carry out immersion treatment with the concentrated sulfuric acid, check the imbibition rate and the water suction curves of seed then according to a conventional method, wherein concentrated sulfuric acid concentration is 98%, soak time 30min, and the imbibition rate of seed is the concentrated sulfuric acid 100%;
B, the seed after step a soaked utilize seed imbibition process and histology thereof after the ESEM Real Time Observation concentrated sulfuric acid is handled to change, and determine that finally dormancy abolishes weak part, the i.e. position at " mouth of a river ".
Embodiment 4
A, the Zhunger Basin of fresh maturation is not had leaf beans seed, carry out immersion treatment with hot water, check the imbibition rate and the water suction curves of seed then according to a conventional method, wherein the hot water temperature is 95 ℃, and soak time is 18min, and the imbibition rate of seed is a hot water 27.11 ± 5.4%;
B, the seed after step a soaked utilize seed imbibition process and histology thereof after the ESEM Real Time Observation hot water treatment to change, and determine that finally dormancy abolishes weak part, the i.e. position at " mouth of a river ".
Embodiment 5
A, the Zhunger Basin of fresh maturation there is not leaf beans seed, carry out immersion treatment with hot water, check the imbibition rate and the water suction curves of seed then according to a conventional method, wherein the hot water temperature is 100 ℃, soak time is 20min, and the imbibition rate of seed is respectively hot water 27.11 ± 5.4%;
B, the seed after step a soaked utilize seed imbibition process and histology thereof after the ESEM Real Time Observation hot water treatment to change, and determine that finally dormancy abolishes weak part, the i.e. position at " mouth of a river ".
Embodiment 6
A, the Zhunger Basin of fresh maturation is not had leaf beans seed, carry out immersion treatment with the concentrated sulfuric acid, check the imbibition rate and the water suction curves of seed then according to a conventional method, wherein concentrated sulfuric acid concentration is 98%, and the imbibition rate of soak time 32min seed is the concentrated sulfuric acid 100%;
B, the seed after step a soaked utilize seed imbibition process and histology thereof after the ESEM Real Time Observation concentrated sulfuric acid is handled to change, and determine that finally dormancy abolishes weak part, the i.e. position at " mouth of a river ".
By method of the present invention, the seed kind leatherware that shows fresh maturation has fluid-tight physical property dormancy phenomenon, plant skin densification (Fig. 2, A, B, C), after the concentrated sulfuric acid processing through 2min, narrow thin crack occurs in the hilarregion of seed, other positions of seed do not have marked change (Fig. 2, D, E); After handling through the concentrated sulfuric acid of 5min, it is big that the crack of hilarregion becomes, and unusual fluctuation appears in the epidermis at the strophiole position, epidermal tissue have slight protuberance (Fig. 2, F, G); After handling through the concentrated sulfuric acid of 10min, the histocyte of hilarregion breaks, and it is big that the crack becomes, epidermal tissue's protuberance at strophiole position obviously (Fig. 2, H, I); After handling through the concentrated sulfuric acid of 15min, the skin breakage around the hilarregion, the hilum crack further increases, epidermal tissue's protuberance at strophiole position obviously, and occur partial rupture (Fig. 2, J, K, L); After handling through the concentrated sulfuric acid of 20min, the skin breakage of hilarregion is serious, and slight crack is deepened, and it is big that the hilum crack becomes, the skin breakage of strophiole position, occur tangible slight crack (Fig. 2, M, N).The above time period removes outside hilum and the strophiole position, and other epidermal tissue does not change, and after handling through the concentrated sulfuric acid of 30min, hilum breaks fully, and strophiole breaks, other epidermal tissue break (Fig. 2, O, P, Q, R), this moment, moisture can enter seed rapidly.Therefore the concentrated sulfuric acid is handled 30min and is abolished the optimization process time that Zhunger Basin does not have leaf beans seed physical property;
Behind hot water treatment 5min, the hilarregion is some cracking slightly, strophiole also slightly some projection (Fig. 3, A, B); Behind the hot water treatment 10min, cracking around the hilum cracking, hilum, strophiole outstanding obviously (Fig. 3, C, D); Behind the hot water treatment 20min, hilum cracking obviously, hilum surrounding tissue slight crack is outstanding, hilum occur slight crack (Fig. 3, E, F).The above time period removes outside hilum and the strophiole position, and other epidermal tissue does not change, and illustrates that hot water treatment does not have the concentrated sulfuric acid strong to the effect of abolishing of seed physical property dormancy;
In addition, verify seed suction dynamic change further combined with seed imbibition curve (Fig. 1), select running water (10h), (95 ℃ of hot water, 20min), sodium hydroxide solution (1%, 30min), manually scratch or the concentrated sulfuric acid (98%, 30min) wait processing, the imbibition rate and the water suction curves of check seed, its imbibition rate is respectively 3.3 ± 1.2%, 27.11 ± 5.4%, 3.41 ± 1.0%, 100%, 100%, water suction curves shows, as time passes, manually scratch the seed imbibition curve of handling with the concentrated sulfuric acid and raise the soonest, the seed imbibition curve of hot water treatment is placed in the middle, running water and sodium hydroxide solution are to seed imbibition do not make significant difference (Fig. 1), illustrate manually to scratch and thoroughly effectively to break the dormancy of seed physical property, but waste time and energy, handle 30min so the best method of breaking the dormancy of seed physical property is the concentrated sulfuric acid because of manually scratching with the concentrated sulfuric acid;
By the imbibition response of ESEM Real Time Observation seed special construction (hilum, strophiole), determine that the weakest position " mouth of a river " is a hilum, behind the hilum cracking, strophiole breaks, and moisture enters seed in a large number and finishes the imbibition process.Learn intensity of variation from seed imbibition process organization, hot water treatment does not have the concentrated sulfuric acid strong to the effect of abolishing of seed physical property dormancy.
Claims (2)
1. a Zhunger Basin does not have the manual method that the dormancy of leaf beans seed physical property discharges, and it is characterized in that following these steps to carrying out:
A, the Zhunger Basin of fresh maturation is not had leaf beans seed, carry out immersion treatment, check the imbibition rate and the water suction curves of seed then according to a conventional method with the hot water or the concentrated sulfuric acid, wherein hot water temperature 90-100 ℃, soak time is 15-20min, and concentrated sulfuric acid concentration is 98%, soak time 28-32min;
B, the seed after step a soaked utilize seed imbibition process and histology thereof after the ESEM Real Time Observation hot water and the concentrated sulfuric acid are handled to change, and determine that finally dormancy abolishes weak part, the i.e. position at " mouth of a river ".
2. method according to claim 1 is characterized in that the imbibition rate of step a seed is respectively hot water 27.11 ± 5.4%, the concentrated sulfuric acid 100%.
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| CN2011101588360A CN102282928A (en) | 2011-06-14 | 2011-06-14 | Artificial method for physical dormancy release of Zunghar leaf-free bean seeds |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0549309A (en) * | 1991-08-27 | 1993-03-02 | Sumitomo Chem Co Ltd | Method for improving germination of seed |
| JPH0870625A (en) * | 1994-09-02 | 1996-03-19 | Sumitomo Chem Co Ltd | Improvement of germination of hard seed |
| JP2004129614A (en) * | 2002-10-11 | 2004-04-30 | Takii Shubyo Kk | Method for improving germination of seed, germination improved seed and coated seed |
| CN101422111A (en) * | 2008-11-04 | 2009-05-06 | 中国科学院新疆生态与地理研究所 | Artificial breeding technique of perennial herb |
| CN101518179A (en) * | 2009-04-01 | 2009-09-02 | 兰州大学 | Method for breaking hard crust of legume seeds |
-
2011
- 2011-06-14 CN CN2011101588360A patent/CN102282928A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0549309A (en) * | 1991-08-27 | 1993-03-02 | Sumitomo Chem Co Ltd | Method for improving germination of seed |
| JPH0870625A (en) * | 1994-09-02 | 1996-03-19 | Sumitomo Chem Co Ltd | Improvement of germination of hard seed |
| JP2004129614A (en) * | 2002-10-11 | 2004-04-30 | Takii Shubyo Kk | Method for improving germination of seed, germination improved seed and coated seed |
| CN101422111A (en) * | 2008-11-04 | 2009-05-06 | 中国科学院新疆生态与地理研究所 | Artificial breeding technique of perennial herb |
| CN101518179A (en) * | 2009-04-01 | 2009-09-02 | 兰州大学 | Method for breaking hard crust of legume seeds |
Non-Patent Citations (3)
| Title |
|---|
| 张道远 等: "准噶尔无叶豆的保护生物学研究", 《干旱区研究》, vol. 28, no. 1, 31 January 2011 (2011-01-31), pages 104 - 110 * |
| 王烨: "14种荒漠珍稀濒危植物的种子特性", 《种子》, no. 3, 30 June 1991 (1991-06-30), pages 23 - 26 * |
| 马文宝 等: "准噶尔无叶豆地理种群种子萌发特性", 《干旱区地理》, vol. 30, no. 5, 30 September 2007 (2007-09-30), pages 674 - 679 * |
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Application publication date: 20111221 |