CN102272334B - 新基因组测序策略 - Google Patents

新基因组测序策略 Download PDF

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Publication number
CN102272334B
CN102272334B CN201080004824.6A CN201080004824A CN102272334B CN 102272334 B CN102272334 B CN 102272334B CN 201080004824 A CN201080004824 A CN 201080004824A CN 102272334 B CN102272334 B CN 102272334B
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China
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sequence
fragment
joint
contig
dna
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CN201080004824.6A
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English (en)
Chinese (zh)
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CN102272334A (zh
Inventor
M·J·T·范艾杰克
A·J·范托恩
A·A·A·W·扬森
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Keygene NV
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Keygene NV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201080004824.6A 2009-01-13 2010-01-13 新基因组测序策略 Expired - Fee Related CN102272334B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US14428109P 2009-01-13 2009-01-13
US61/144,281 2009-01-13
US22646809P 2009-07-17 2009-07-17
NL2003235 2009-07-17
US61/226,468 2009-07-17
NL2003235 2009-07-17
PCT/NL2010/000003 WO2010082815A1 (en) 2009-01-13 2010-01-13 Novel genome sequencing strategies

Publications (2)

Publication Number Publication Date
CN102272334A CN102272334A (zh) 2011-12-07
CN102272334B true CN102272334B (zh) 2014-08-20

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ID=41277440

Family Applications (1)

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CN201080004824.6A Expired - Fee Related CN102272334B (zh) 2009-01-13 2010-01-13 新基因组测序策略

Country Status (6)

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US (1) US20130196859A1 (https=)
EP (1) EP2379751B1 (https=)
JP (1) JP2012514977A (https=)
CN (1) CN102272334B (https=)
ES (1) ES2403312T3 (https=)
WO (1) WO2010082815A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2513333B1 (en) * 2009-12-17 2013-10-02 Keygene N.V. Restriction enzyme based whole genome sequencing
FR2968671B1 (fr) * 2010-12-13 2015-05-01 Chaouki Miled Sequencage maspet
WO2013152505A1 (zh) * 2012-04-13 2013-10-17 深圳华大基因科技服务有限公司 一种转录组组装的方法及系统
CN104781421B (zh) * 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
JP2015530096A (ja) * 2012-09-24 2015-10-15 シービー バイオテクノロジーズ インコーポレイテッド 非干渉性、ノイズキャンセル性のポリヌクレオチド識別タグを用いたマルチプレックスパイロシーケンシング
CN102982252A (zh) * 2012-12-05 2013-03-20 北京诺禾致源生物信息科技有限公司 一种高杂合二倍体基因组支架序列组装策略
CA2908361C (en) * 2013-04-17 2023-09-26 Pioneer Hi-Bred International, Inc. Methods for characterizing dna sequence composition in a genome
CN103388025B (zh) * 2013-07-10 2015-04-29 华中农业大学 基于克隆dna混合池的全基因组测序方法
CN105874082B (zh) 2013-10-07 2020-06-02 塞昆纳姆股份有限公司 用于非侵入性评估染色体改变的方法和过程
JP6418800B2 (ja) * 2014-06-17 2018-11-07 国立大学法人 東京大学 外来性ｄｎａのゲノムへの導入位置の決定法
US9710296B2 (en) 2014-12-09 2017-07-18 Vmware, Inc. Allocating cost of disk usage to a linked clone virtual machine
US9582309B2 (en) 2014-12-09 2017-02-28 Vmware, Inc. Allocating cost of disk usage to a linked clone virtual machine based on a parameter of usage
WO2016134034A1 (en) 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
CN109817280B (zh) * 2016-04-06 2023-04-14 晶能生物技术（上海）有限公司 一种测序数据组装方法
CN106778060B (zh) * 2016-10-09 2019-05-21 南京双运生物技术有限公司 一种利用原核生物基因组高质量草图制作完成图的方法
CN108660197A (zh) * 2017-04-01 2018-10-16 深圳华大基因科技服务有限公司 一种二代序列基因组重叠群的组装方法和系统

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008007951A1 (en) * 2006-07-12 2008-01-17 Keygene N.V. High throughput physical mapping using aflp

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU672760B2 (en) 1991-09-24 1996-10-17 Keygene N.V. Selective restriction fragment amplification: a general method for DNA fingerprinting
WO2000006770A1 (en) 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
CA2345441A1 (en) 1998-10-27 2000-05-04 Affymetrix, Inc. Complexity management and analysis of genomic dna
EP1131153A1 (en) 1998-11-06 2001-09-12 Solexa Ltd. A method for reproducing molecular arrays
WO2000058507A1 (en) 1999-03-30 2000-10-05 Solexa Ltd. Polynucleotide sequencing
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
DE60222628T2 (de) 2001-01-30 2008-07-17 Solexa Ltd., Saffron Walden Herstellung von matrizen aus polynukleotiden
US7809509B2 (en) 2001-05-08 2010-10-05 Ip Genesis, Inc. Comparative mapping and assembly of nucleic acid sequences
WO2003004690A2 (en) 2001-07-06 2003-01-16 454$m(3) CORPORATION Method for isolation of independent, parallel chemical micro-reactions using a porous filter
GB0119719D0 (en) 2001-08-13 2001-10-03 Solexa Ltd DNA sequence analysis
US6975943B2 (en) 2001-09-24 2005-12-13 Seqwright, Inc. Clone-array pooled shotgun strategy for nucleic acid sequencing
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
PT3438116T (pt) 2002-08-23 2021-03-23 Illumina Cambridge Ltd Nucleótidos identificados
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
EP1567669B1 (en) 2002-12-02 2010-03-24 Illumina Cambridge Limited Determination of methylation of nucleic acid sequences
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
GB0304371D0 (en) 2003-02-26 2003-04-02 Solexa Ltd DNA Sequence analysis
GB0320059D0 (en) 2003-08-27 2003-10-01 Solexa Ltd A method of sequencing
GB0326073D0 (en) 2003-11-07 2003-12-10 Solexa Ltd Improvements in or relating to polynucleotide arrays
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
GB0400974D0 (en) 2004-01-16 2004-02-18 Solexa Ltd Multiple inexact matching
GB0402895D0 (en) 2004-02-10 2004-03-17 Solexa Ltd Arrayed polynucleotides
DE602006013831D1 (de) 2005-06-23 2010-06-02 Keygene Nv Verbesserte strategien zur sequenzierung komplexer genome unter verwendung von sequenziertechniken mit hohem durchsatz
US20070082358A1 (en) 2005-10-11 2007-04-12 Roderic Fuerst Sequencing by synthesis based ordered restriction mapping
WO2010036287A1 (en) * 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008007951A1 (en) * 2006-07-12 2008-01-17 Keygene N.V. High throughput physical mapping using aflp

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Shotgun sequencing, clone pooling, and comparative strategies for mapping and sequencing;Aleksandar Milosavljevic;《TARGETS》;20031231;第12卷(第6期);246-252 *
The Genome of the Sea Urchin Strongylocentrotus purpuratus;Sea Urchin Genome Sequencing Consortium;《SCIENCE》;20061110;第314卷;942 *

Also Published As

Publication number Publication date
WO2010082815A1 (en) 2010-07-22
US20130196859A1 (en) 2013-08-01
ES2403312T3 (es) 2013-05-17
CN102272334A (zh) 2011-12-07
EP2379751B1 (en) 2013-03-20
EP2379751A1 (en) 2011-10-26
JP2012514977A (ja) 2012-07-05

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