CN102257149B - 由超产酵母生产角鲨烯 - Google Patents

由超产酵母生产角鲨烯 Download PDF

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Publication number
CN102257149B
CN102257149B CN2009801391118A CN200980139111A CN102257149B CN 102257149 B CN102257149 B CN 102257149B CN 2009801391118 A CN2009801391118 A CN 2009801391118A CN 200980139111 A CN200980139111 A CN 200980139111A CN 102257149 B CN102257149 B CN 102257149B
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China
Prior art keywords
squalene
yeast
emulsion
oil
described yeast
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Chinese (zh)
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CN102257149A (zh
Inventor
M·布鲁克
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Si Qile
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Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/113—Multiple emulsions, e.g. oil-in-water-in-oil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Dispersion Chemistry (AREA)
  • Epidemiology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Enzymes And Modification Thereof (AREA)
CN2009801391118A 2008-08-28 2009-08-28 由超产酵母生产角鲨烯 Active CN102257149B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19048608P 2008-08-28 2008-08-28
US61/190,486 2008-08-28
PCT/IB2009/006825 WO2010023551A2 (en) 2008-08-28 2009-08-28 Production of squalene from hyper-producing yeasts

Publications (2)

Publication Number Publication Date
CN102257149A CN102257149A (zh) 2011-11-23
CN102257149B true CN102257149B (zh) 2013-12-25

Family

ID=41625158

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801391118A Active CN102257149B (zh) 2008-08-28 2009-08-28 由超产酵母生产角鲨烯

Country Status (23)

Country Link
US (2) US11236366B2 (enExample)
EP (2) EP2369003B1 (enExample)
JP (3) JP4938911B2 (enExample)
KR (1) KR101443064B1 (enExample)
CN (1) CN102257149B (enExample)
AT (1) ATE513920T1 (enExample)
AU (1) AU2009286426B2 (enExample)
BR (1) BRPI0917716A2 (enExample)
CA (1) CA2735404C (enExample)
CY (1) CY1114687T1 (enExample)
DK (1) DK2268823T3 (enExample)
EA (1) EA028898B1 (enExample)
ES (2) ES2761697T3 (enExample)
HR (1) HRP20110671T1 (enExample)
IL (1) IL211435A (enExample)
MX (1) MX2011002279A (enExample)
NZ (1) NZ591876A (enExample)
PL (1) PL2268823T3 (enExample)
PT (1) PT2268823E (enExample)
RS (1) RS51999B (enExample)
SI (1) SI2268823T1 (enExample)
SM (1) SMT201100058B (enExample)
WO (1) WO2010023551A2 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
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US10100334B2 (en) 2008-05-23 2018-10-16 Nucelis Inc. Production of squalene using yeast
EP2292741A1 (en) * 2009-08-26 2011-03-09 OrganoBalance GmbH Genetically modified organisms for the production of lipids
JP5978132B2 (ja) 2009-11-23 2016-08-24 ニューセリス インコーポレイテッド 酵母を用いるスクアレン生成のための方法および組成物
JP5946188B2 (ja) 2010-05-12 2016-07-05 ノバルティス アーゲー スクアレンを調製するための改良された方法
CN102100260B (zh) * 2010-12-03 2013-07-31 滨州学院 酵母油脂及其制备方法和应用
CN102787140A (zh) 2011-05-20 2012-11-21 罗盖特兄弟公司 从微藻制备与提取角鲨烯的工艺方法
CN102787074B (zh) 2011-05-20 2016-06-08 罗盖特兄弟公司 生产角鲨烯的微藻新菌株
FR2975705B1 (fr) 2011-05-27 2014-12-26 Roquette Freres Procede d'extraction du squalene a partir de microalgues
US9375471B2 (en) 2012-03-08 2016-06-28 Glaxosmithkline Biologicals Sa Adjuvanted formulations of booster vaccines
IN2014DN08554A (enExample) * 2012-04-16 2015-05-15 Roquette Freres
FR2989373B1 (fr) * 2012-04-16 2016-05-06 Roquette Freres Procede de raffinage du squalene produit par microalgues
RU2015106930A (ru) 2012-09-06 2016-10-20 Новартис Аг Комбинированные вакцины с менингококком серогруппы в и к/д/с
EP2906239A1 (en) 2012-10-12 2015-08-19 GlaxoSmithKline Biologicals SA Non-cross-linked acellular pertussis antigens for use in combination vaccines
US20150320852A1 (en) 2012-12-18 2015-11-12 Glaxosmithkline Biologicals Sa Conjugates for protecting against diphtheria and/or tetanus
EP2971049B1 (en) 2013-03-13 2017-03-01 Sanofi-Aventis Deutschland GmbH Production of squalene and/or sterol from cell suspensions of fermented yeast
GB2518927A (en) * 2013-05-21 2015-04-08 Univ Swansea The production of biomolecules using yeast
CN103849643A (zh) * 2014-03-11 2014-06-11 北京理工大学 一种利用蛋白支架提高酿酒酵母中鲨烯的合成方法
CA2948122C (en) 2014-04-09 2024-05-14 Adeka Corporation Mutant enzyme and production method for terpenoid using said mutant enzyme
EP3225691B1 (en) * 2014-11-28 2020-10-28 Ajinomoto Co., Inc. Method for producing isoprenoid compound
ES2861806T3 (es) 2014-12-02 2021-10-06 Novartis Ag Fabricación de composiciones que contienen tensioactivo
CN104560731B (zh) * 2014-12-26 2018-10-09 中国科学院青岛生物能源与过程研究所 一种高产角鲨烯的类酵母菌及其应用
CN110373338B (zh) * 2019-08-15 2021-09-28 宜昌东阳光生化制药有限公司 酿酒酵母及其用途
CN111893049A (zh) * 2020-06-30 2020-11-06 厦门大学 一种过表达角鲨烯合成酶基因的裂殖壶菌基因工程菌株及其构建方法和应用
WO2025053947A1 (en) * 2023-09-07 2025-03-13 Avantor Performance Materials, Llc Synthetic triterpenes composition, method of production and applications thereof

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HU212924B (en) 1989-05-25 1996-12-30 Chiron Corp Adjuvant formulation comprising a submicron oil droplet emulsion
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
JP2785084B2 (ja) 1991-11-18 1998-08-13 三菱石油株式会社 スクアレンを生産する酵母の作製法及び作製された新規酵母
JPH06116171A (ja) 1992-10-06 1994-04-26 Nippon Fine Chem Co Ltd 天然スクワレンの精製方法
DE4316620A1 (de) 1993-05-18 1994-11-24 Mueller Extract Co Gmbh Verfahren und Vorrichtung zur Herstellung von Squalen aus Olivenölrückständen
GB9326253D0 (en) 1993-12-23 1994-02-23 Smithkline Beecham Biolog Vaccines
JPH10120548A (ja) 1996-10-23 1998-05-12 Shiseido Co Ltd 肌荒れ防止剤
DE19744212B4 (de) * 1997-09-30 2006-01-19 Schering Ag Verfahren zur Herstellung von Ergosterol und dessen Zwischenprodukten mittels rekombinanter Hefen
US20040235088A1 (en) 1997-09-30 2004-11-25 Schering Aktiengesellschaft Process for the production of ergosterol and its intermediate products using recombinant yeasts
DE10012370A1 (de) * 2000-03-14 2001-09-27 Chiron Behring Gmbh & Co Adjuvans für Vakzinen
US20020168389A1 (en) * 2000-12-28 2002-11-14 Prem Chandar Stable skin conditioning compositions containing retinoid boosters
AU2004297299A1 (en) * 2003-12-12 2005-06-23 Sanofi Pasteur Limited Production of diphtheria toxin
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
US20060286046A1 (en) 2005-01-05 2006-12-21 Haber C Andrew Skin care compositions
CN1930186A (zh) * 2005-03-03 2007-03-14 阿勒根公司 为获得梭菌毒素的梭菌细菌培养基及方法
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DK1909830T3 (da) 2005-08-02 2011-12-19 Novartis Vaccines & Diagnostic Formindskelse af interferens mellem olieholdige adjuvanser og antigener indeholdende overfladeaktivt middel
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US10100334B2 (en) * 2008-05-23 2018-10-16 Nucelis Inc. Production of squalene using yeast
EP2292741A1 (en) 2009-08-26 2011-03-09 OrganoBalance GmbH Genetically modified organisms for the production of lipids

Also Published As

Publication number Publication date
ATE513920T1 (de) 2011-07-15
JP2012500648A (ja) 2012-01-12
ES2761697T3 (es) 2020-05-20
JP5930766B2 (ja) 2016-06-08
JP2015061546A (ja) 2015-04-02
BRPI0917716A2 (pt) 2019-09-03
PT2268823E (pt) 2011-09-01
HK1152078A1 (en) 2012-02-17
IL211435A0 (en) 2011-05-31
CY1114687T1 (el) 2016-10-05
JP2012139225A (ja) 2012-07-26
PL2268823T3 (pl) 2012-03-30
EP2369003B1 (en) 2019-10-16
EP2268823B1 (en) 2011-06-22
SI2268823T1 (sl) 2011-10-28
US11236366B2 (en) 2022-02-01
SMT201100058B (it) 2012-01-18
DK2268823T3 (da) 2011-10-03
CA2735404C (en) 2013-04-16
MX2011002279A (es) 2011-04-21
IL211435A (en) 2014-03-31
NZ591876A (en) 2013-05-31
WO2010023551A3 (en) 2010-04-29
AU2009286426A1 (en) 2010-03-04
KR20110049887A (ko) 2011-05-12
HRP20110671T1 (hr) 2011-11-30
EP2369003A1 (en) 2011-09-28
EA201170381A1 (ru) 2011-08-30
RS51999B (sr) 2012-04-30
EP2268823A2 (en) 2011-01-05
JP4938911B2 (ja) 2012-05-23
KR101443064B1 (ko) 2014-09-19
ES2366941T3 (es) 2011-10-26
AU2009286426B2 (en) 2013-08-22
US20220177928A1 (en) 2022-06-09
EA028898B1 (ru) 2018-01-31
CA2735404A1 (en) 2010-03-04
CN102257149A (zh) 2011-11-23
US20110243969A1 (en) 2011-10-06
WO2010023551A2 (en) 2010-03-04

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Legal Events

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C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent of invention or patent application
CB02 Change of applicant information

Address after: Basel, Switzerland

Applicant after: NOVARTIS AG

Address before: Basel, Switzerland

Applicant before: NOVARTIS AG

COR Change of bibliographic data

Free format text: CORRECT: APPLICANT; FROM: NOVARTIS VACCINES + DIAGNOSTIC TO: NOVARTIS CO., LTD.

C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20241120

Address after: Burke County, England

Patentee after: Si Qile

Country or region after: Britain

Address before: Basel, SUI

Patentee before: NOVARTIS AG

Country or region before: Switzerland

TR01 Transfer of patent right