EA201170381A1 - Получение сквалена из гиперпродуцирующих дрожжей - Google Patents

Получение сквалена из гиперпродуцирующих дрожжей

Info

Publication number
EA201170381A1
EA201170381A1 EA201170381A EA201170381A EA201170381A1 EA 201170381 A1 EA201170381 A1 EA 201170381A1 EA 201170381 A EA201170381 A EA 201170381A EA 201170381 A EA201170381 A EA 201170381A EA 201170381 A1 EA201170381 A1 EA 201170381A1
Authority
EA
Eurasian Patent Office
Prior art keywords
hyper
squal
getting
squalene
yeast
Prior art date
Application number
EA201170381A
Other languages
English (en)
Other versions
EA028898B1 (ru
Inventor
Михаэль Брекер
Original Assignee
Новартис Аг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41625158&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA201170381(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Новартис Аг filed Critical Новартис Аг
Publication of EA201170381A1 publication Critical patent/EA201170381A1/ru
Publication of EA028898B1 publication Critical patent/EA028898B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/113—Multiple emulsions, e.g. oil-in-water-in-oil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes

Abstract

Описан способ получения очищенных дрожжей, где источником сквалена являются дрожжи, которые гиперпродуцируют сквален. Сквален используют в фармацевтических целях. Например, его можно использовать для получения эмульсии вода-в-масле, и эмульсия является особенно подходящей для применения в качестве иммунологического адьюванта.
EA201170381A 2008-08-28 2009-08-28 Получение сквалена из гиперпродуцирующих дрожжей EA028898B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19048608P 2008-08-28 2008-08-28
PCT/IB2009/006825 WO2010023551A2 (en) 2008-08-28 2009-08-28 Production of squalene from hyper-producing yeasts

Publications (2)

Publication Number Publication Date
EA201170381A1 true EA201170381A1 (ru) 2011-08-30
EA028898B1 EA028898B1 (ru) 2018-01-31

Family

ID=41625158

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201170381A EA028898B1 (ru) 2008-08-28 2009-08-28 Получение сквалена из гиперпродуцирующих дрожжей

Country Status (24)

Country Link
US (2) US11236366B2 (ru)
EP (2) EP2369003B1 (ru)
JP (3) JP4938911B2 (ru)
KR (1) KR101443064B1 (ru)
CN (1) CN102257149B (ru)
AT (1) ATE513920T1 (ru)
AU (1) AU2009286426B2 (ru)
BR (1) BRPI0917716A2 (ru)
CA (1) CA2735404C (ru)
CY (1) CY1114687T1 (ru)
DK (1) DK2268823T3 (ru)
EA (1) EA028898B1 (ru)
ES (2) ES2761697T3 (ru)
HK (1) HK1152078A1 (ru)
HR (1) HRP20110671T1 (ru)
IL (1) IL211435A (ru)
MX (1) MX2011002279A (ru)
NZ (1) NZ591876A (ru)
PL (1) PL2268823T3 (ru)
PT (1) PT2268823E (ru)
RS (1) RS51999B (ru)
SI (1) SI2268823T1 (ru)
SM (1) SMT201100058B (ru)
WO (1) WO2010023551A2 (ru)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA201071338A1 (ru) 2008-05-23 2012-01-30 Сибас Ойлз, Ллс Получение сквалена с применением дрожжей
EP2292741A1 (en) * 2009-08-26 2011-03-09 OrganoBalance GmbH Genetically modified organisms for the production of lipids
WO2011063350A2 (en) 2009-11-23 2011-05-26 Cibus Oils, Llc Methods and compositions for producing squalene using yeast
NZ603918A (en) 2010-05-12 2015-04-24 Novartis Ag Improved methods for preparing squalene
CN102100260B (zh) * 2010-12-03 2013-07-31 滨州学院 酵母油脂及其制备方法和应用
CN102787140A (zh) 2011-05-20 2012-11-21 罗盖特兄弟公司 从微藻制备与提取角鲨烯的工艺方法
CN102787074B (zh) 2011-05-20 2016-06-08 罗盖特兄弟公司 生产角鲨烯的微藻新菌株
FR2975705B1 (fr) 2011-05-27 2014-12-26 Roquette Freres Procede d'extraction du squalene a partir de microalgues
RU2014140521A (ru) 2012-03-08 2016-04-27 Новартис Аг Адъювантные составы бустерных вакцин
IN2014DN08554A (ru) * 2012-04-16 2015-05-15 Roquette Freres
FR2989373B1 (fr) * 2012-04-16 2016-05-06 Roquette Freres Procede de raffinage du squalene produit par microalgues
CA2882619A1 (en) 2012-09-06 2014-03-13 Novartis Ag Combination vaccines with serogroup b meningococcus and d/t/p
AU2013328548A1 (en) 2012-10-12 2015-05-07 Glaxosmithkline Biologicals Sa Non-cross-linked acellular pertussis antigens for use in combination vaccines
CN105007935A (zh) 2012-12-18 2015-10-28 葛兰素史密丝克莱恩生物有限公司 用于保护免于白喉和/或破伤风的缀合物
EP2971049B1 (en) 2013-03-13 2017-03-01 Sanofi-Aventis Deutschland GmbH Production of squalene and/or sterol from cell suspensions of fermented yeast
GB2518927A (en) * 2013-05-21 2015-04-08 Univ Swansea The production of biomolecules using yeast
CN103849643A (zh) * 2014-03-11 2014-06-11 北京理工大学 一种利用蛋白支架提高酿酒酵母中鲨烯的合成方法
WO2015156369A1 (ja) * 2014-04-09 2015-10-15 株式会社Adeka 変異酵素及び前記変異酵素を用いたテルペノイドの製造方法
EP3225691B1 (en) * 2014-11-28 2020-10-28 Ajinomoto Co., Inc. Method for producing isoprenoid compound
ES2861806T3 (es) 2014-12-02 2021-10-06 Novartis Ag Fabricación de composiciones que contienen tensioactivo
CN104560731B (zh) * 2014-12-26 2018-10-09 中国科学院青岛生物能源与过程研究所 一种高产角鲨烯的类酵母菌及其应用
CN110373338B (zh) * 2019-08-15 2021-09-28 宜昌东阳光生化制药有限公司 酿酒酵母及其用途
CN111893049A (zh) * 2020-06-30 2020-11-06 厦门大学 一种过表达角鲨烯合成酶基因的裂殖壶菌基因工程菌株及其构建方法和应用

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0832638B2 (ja) 1989-05-25 1996-03-29 カイロン コーポレイション サブミクロン油滴乳剤を含んで成るアジュバント製剤
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
JP2785084B2 (ja) 1991-11-18 1998-08-13 三菱石油株式会社 スクアレンを生産する酵母の作製法及び作製された新規酵母
JPH06116171A (ja) 1992-10-06 1994-04-26 Nippon Fine Chem Co Ltd 天然スクワレンの精製方法
DE4316620A1 (de) 1993-05-18 1994-11-24 Mueller Extract Co Gmbh Verfahren und Vorrichtung zur Herstellung von Squalen aus Olivenölrückständen
GB9326253D0 (en) 1993-12-23 1994-02-23 Smithkline Beecham Biolog Vaccines
JPH10120548A (ja) 1996-10-23 1998-05-12 Shiseido Co Ltd 肌荒れ防止剤
US20040235088A1 (en) 1997-09-30 2004-11-25 Schering Aktiengesellschaft Process for the production of ergosterol and its intermediate products using recombinant yeasts
DE19744212B4 (de) * 1997-09-30 2006-01-19 Schering Ag Verfahren zur Herstellung von Ergosterol und dessen Zwischenprodukten mittels rekombinanter Hefen
DE10012370A1 (de) * 2000-03-14 2001-09-27 Chiron Behring Gmbh & Co Adjuvans für Vakzinen
US20020168389A1 (en) * 2000-12-28 2002-11-14 Prem Chandar Stable skin conditioning compositions containing retinoid boosters
WO2006096164A1 (en) * 2005-03-03 2006-09-14 Allergan, Inc. Media for clostridium bacterium and processes for obtaining a clostridial toxin
WO2005056773A1 (en) * 2003-12-12 2005-06-23 Sanofi Pasteur Limited Production of diphtheria toxin
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
US20060286046A1 (en) 2005-01-05 2006-12-21 Haber C Andrew Skin care compositions
US8703095B2 (en) 2005-07-07 2014-04-22 Sanofi Pasteur S.A. Immuno-adjuvant emulsion
US7375188B2 (en) * 2005-07-29 2008-05-20 Mallinckrodt Baker, Inc. Vegetarian protein a preparation and methods thereof
EA014314B1 (ru) 2005-08-02 2010-10-29 Новартис Вэксинес Энд Дайэгностикс Срл Пониженная интерференция между маслосодержащими адъювантами и антигенами, содержащими поверхностно-активные вещества
JP4210674B2 (ja) 2005-08-31 2009-01-21 全国農業協同組合連合会 マイコプラズマ・ガリセプティカム培養用培地
FR2896162B1 (fr) 2006-01-13 2008-02-15 Sanofi Pasteur Sa Emulsion huile dans eau thermoreversible
JP5053557B2 (ja) * 2006-03-24 2012-10-17 株式会社 資生堂 白濁状水中油型乳化化粧料の製造方法
SI2484375T1 (en) * 2006-09-26 2018-08-31 Infectious Disease Research Institute A vaccine composition comprising a synthetic adjuvant
WO2008130372A2 (en) 2006-09-28 2008-10-30 Microbia, Inc. Production of sterols in oleaginous yeast and fungi
BRPI0717219B8 (pt) 2006-10-12 2021-05-25 Glaxosmithkline Biologicals Sa composição imunogênica, e, uso de uma composição imunogênica
GB0622282D0 (en) * 2006-11-08 2006-12-20 Novartis Ag Quality control methods
US8092813B1 (en) * 2007-12-28 2012-01-10 Novartis Ag Polychlorinated biphenyls and squalene-containing adjuvants
EA201071338A1 (ru) * 2008-05-23 2012-01-30 Сибас Ойлз, Ллс Получение сквалена с применением дрожжей
EP2292741A1 (en) 2009-08-26 2011-03-09 OrganoBalance GmbH Genetically modified organisms for the production of lipids

Also Published As

Publication number Publication date
EP2268823B1 (en) 2011-06-22
MX2011002279A (es) 2011-04-21
US20220177928A1 (en) 2022-06-09
PT2268823E (pt) 2011-09-01
ES2761697T3 (es) 2020-05-20
WO2010023551A2 (en) 2010-03-04
CA2735404C (en) 2013-04-16
EA028898B1 (ru) 2018-01-31
US11236366B2 (en) 2022-02-01
SMT201100058B (it) 2012-01-18
HK1152078A1 (en) 2012-02-17
WO2010023551A3 (en) 2010-04-29
JP4938911B2 (ja) 2012-05-23
RS51999B (en) 2012-04-30
CA2735404A1 (en) 2010-03-04
KR101443064B1 (ko) 2014-09-19
SI2268823T1 (sl) 2011-10-28
IL211435A (en) 2014-03-31
EP2369003B1 (en) 2019-10-16
PL2268823T3 (pl) 2012-03-30
IL211435A0 (en) 2011-05-31
AU2009286426B2 (en) 2013-08-22
CN102257149B (zh) 2013-12-25
DK2268823T3 (da) 2011-10-03
US20110243969A1 (en) 2011-10-06
KR20110049887A (ko) 2011-05-12
JP5930766B2 (ja) 2016-06-08
NZ591876A (en) 2013-05-31
CN102257149A (zh) 2011-11-23
CY1114687T1 (el) 2016-10-05
ES2366941T3 (es) 2011-10-26
ATE513920T1 (de) 2011-07-15
EP2268823A2 (en) 2011-01-05
JP2012139225A (ja) 2012-07-26
HRP20110671T1 (hr) 2011-11-30
JP2015061546A (ja) 2015-04-02
EP2369003A1 (en) 2011-09-28
BRPI0917716A2 (pt) 2019-09-03
JP2012500648A (ja) 2012-01-12
AU2009286426A1 (en) 2010-03-04

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