CN102253037A - Test paper slip for testing adulterated milk rapidly - Google Patents
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- CN102253037A CN102253037A CN2011100905325A CN201110090532A CN102253037A CN 102253037 A CN102253037 A CN 102253037A CN 2011100905325 A CN2011100905325 A CN 2011100905325A CN 201110090532 A CN201110090532 A CN 201110090532A CN 102253037 A CN102253037 A CN 102253037A
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Abstract
The invention discloses a test paper slip for testing adulterated milk rapidly. The test paper slip is characterized in that a starch test paper slip for composing the test paper slip is produced by the steps of taking 10 ml of an iodine-potassium iodide solution with an I2 concentration of 0.032 mol/mL and a KI concentration of 0.126 mol/mL, diluting it with equal amount of a solvent, immersing completely quantitative test paper into the diluted iodine-potassium iodide solution for 5 minutes, airing the test paper obtained from the previous step at a temperature of 5 to 6 DEG C in darkness, clipping, and preserving it in a dark place; a carbamide test paper slip for composing the test paper slip is produced by the steps of taking 6 ml of a sodium nitrite hydrochloric acid solution with a sodium nitrite content of 0.04%, adding 0.4 g of a Griess reagent into the sodium nitrite hydrochloric acid solution, mixing well, immersing completely quantitative test paper into the mixture for 10 minutes, taking out the immersed test paper, airing it in the shade, clipping, and preserving it in a dark place; an edible alkali test paper slip for composing the test paper slip is produced by the steps of taking 10 ml of a prepared Bromothymol Blue ethanol indicator with a Bromothymol Blue content of 0.04%, placing the indicator on a disk, immersing completely quantitative test paper into the indicator for 10 to 15 minutes, taking out it, airing it, clipping, and preserving it in a dry place; and a salt test paper slip for composing the test paper slip is produced by the steps of mixing well 15 ml of a silver nitrate solution with a silver nitrate concentration of 0.01 mol/mL and six drops of a potassium chromate solution with a potassium chromate content of 10% quickly, immersing completely quantitative test paper into the mixture for about 20 minutes, taking out it, airing the immersed test paper, clipping, and preserving it in a dry place. The test paper slip provided by the invention can carry out an on-site test on raw milk, has a high sensitivity of test and can produce an accurate test result.
Description
Technical field
The present invention relates to the food inspection field, particularly a kind of milk quick detection test paper bar of mingling.
Background technology
At present, China's food-safety problem becomes increasingly conspicuous, and wherein dairy products are mingled problem and received much attention.The milk source of China most of Milk Production manufacturer all belongs to and is distributed to the family, therefore how to control, check the raw milk quality just to become cow's milk production firm to guarantee the link of its product quality most critical.
Food adulteration is illegally to mix outward appearance, physical behavior or plesiomorphic non-people with the kind material in the sensing food to be behavior.According to investigations, the mode of mingling in the food, mixing mainly contains in food and sneaks into foreign matter, promptly sneaks into the intrinsic material of non-this product in normal food, with the content of the intrinsic material that increases former food; Another kind is palmed certified products off as for inferior goods, palms new fresh milk off as recovery milk.
Nowadays on the market, it is a very ubiquitous problem that fresh milk is mingled, and because of much there not be the fix the price according to the quality milk sheds of milk collection of implementation, is that the proportion according to milk carries out an acceptance inspection.The modal material of mingling mainly contains water, thin rice gruel (starch), urea, dietary alkali, salt, fatty wet goods.A lot of milk sheds or milk plant must purchase at short notice in the face of a large amount of raw material milks, the check of mingling material in the milk need be sampled and be carried out in the laboratory, the degree of online detection is not high, the time that not only needs is longer, process is loaded down with trivial details, and in the process of transportation, influence the cow's milk quality easily, the quality of raw milk can't be ensured.
2008, Sichuan University works out in the cow's milk starch and mingles quick detection test paper, mainly utilization is that starch is met the principle that iodine becomes indigo plant, soak time, immersion reagent concentration and test paper susceptibility to this detection test paper carry out system thinking and optimization, finally obtain in the cow's milk starch and mingle quick detection test paper, and determine that the lowest detection that detects test paper is limited to 0.8mg/L, erroneous judgement property is little.Experimental result shows that this detection detection paper speed is fast, is quick on the draw, and is easy to use, suits to operate in purchase scene, plant and field.
In April, 2009, chemical institute of Central South University has carried out at the modernization of cmm center applied research of near infrared spectrum aspect the milk product component fast detecting.If can accurately check composition in the cow's milk also just can judge naturally whether to be mixed with other in the raw milk and mingle material.Analyze in the research, milk constituents detects still based on chemical analysis method at present, though its assay is accurate, and process time and effort consuming, destruction sample, and be offline inspection.Near infrared spectrum (NIRS) is meant the electromagnetic wave of wavelength in the 780-2526nm scope.Near-infrared spectral analysis technology, it is the combination of spectral measurement methods and Chemical Measurement subject, this technology have cost low, pollution-free, do not have destructive, test favorable reproducibility, sample need not pre-service, can online detection and advantage such as simultaneous determination of multiponents, be successfully applied to agricultural and food industry.
The electric intelligent ultrasound wave milk detecting instrument with the Institute Of Electrical Engineering development of Shaanxi Tech Univ can be analyzed the composition in the milk.The low-yield ultrasonic detecting technology of high frequency (High Frequency Low2intensity Ultrasonic Measurement) is to utilize low-yield (1W/cm2) and frequency is higher than the measuring technique that the ultrasound wave of 100kHz obtains information such as detected material internal structure and physicochemical characteristic.But this techniqueflow complexity can only be measured the part composition in the milk, and the high complicated operation of cost.
Shaanxi Tech Univ's life science and engineering college have the mid-infrared light spectral technology to be widely used in the urea detection, though its analytical performance is worse than other conventional method of analysis, but because its cost performance advantage such as ornamental that have easy logistics progress, batch samples processing power and analysis itself are had, be suitable for very much practical application, so various in a few years infrared analytical instrument floods the market rapidly.But this technology can't be mingled material to other and carry out qualitative or half semiquantitative detection just at Determination of Urea in the cow's milk.
Chinese patent 201010034233.5 discloses " a kind of pick-up unit of milk constituents and method ", utilizes ultrasound wave and near-infrared spectral analysis technology to detect milk constituents exactly.
In March, 2006, mix two kinds of method for quick of urea in the milk by Hebei Prov. Product Quality Monitor and Inspection Inst. research and development, two kinds of methods are respectively two kinds of paradime thylaminobenzaldehyde method and Griess reagent methods.Wherein, the experimental principle of paradime thylaminobenzaldehyde method is, what paradime thylaminobenzaldehyde generated with urea reaction under acid condition is the lemon yellow compound to dimethylamino formaldehyde urea, the milk sample displaing yellow that for this reason adds urea, the fresh milk of not mixing urea is colourless, with the increase of urea amount, color deepens thereupon.According to change in color, but whether mix urea in the qualitative detection cow's milk.The Griess reagent ratio juris then is, urea and nitrite react in acid solution and generate the nitrogen effusion, Griess reagent can react with nitrite generation azo, generate the aubergine compound, the cow's milk that mixes urea can not develop the color owing to the consumption of nitrite, and normal milk is displaing amaranth then.
Whether contain and mingle material though above several method can detect cow's milk effectively, all can not purchase the scene or plant measures institute's raw milk, and some operation steps is complicated, be difficult to realize that the scene of raw milk detects so cost is higher at milking station.
Summary of the invention
The objective of the invention is to overcome above-mentioned shortcoming, provide a kind of and can carry out the test strips that detection is mingled at the scene raw milk, whether this test strips can accurately detect to contain in the raw milk simultaneously mingles material, highly sensitive, accuracy is high and carrying out half half-quantitative detection to mingling material on the basis qualitatively, make the source quality safety of Dairy Production secure.
Mingling described in the present invention belongs to sneaks into foreign matter in food, emphasis is at carrying out qualitative or half-quantitative detection through materials such as common starch, urea, alkali, salt in the raw material milk.
Experimental principle:
Its principle is to adopt different chemical reagent can be respectively to manifest different colours with material generation chemical reaction such as starch, urea, alkali, salt, carry out qualitative and sxemiquantitative research, make test strip, make the above-mentioned material that mixes be determined out within the specific limits.
1. starch test experiment: starch is white amorphous powder, is made up of amylose (accounting for 10-30%) and amylopectin (accounting for 70-90%).Amylose can be dissolved in hot water and not in the pasty state, amylopectin is water insoluble, and hot water acts on it then expanding and forms pasty state.Amylose wherein soluble in water is curve form, and borrows intramolecular hydrogen bond to be curled into spiral fashion.At this moment add the tincture of iodine, wherein iodine molecule just pierces the central space of spiral, and links together by Fan Dehuali and amylose, thereby forms complex compound.This complex compound can absorb other visible light (wavelength coverage is a 400-750 sodium rice) except that blue light more equably, thereby makes starch become mazarine, shown in the content of starch that mixed in the blue raw milk that dark explanation is tested more many more.
2. urea test experience: its experimental principle is nitrite-Griess reagent method, urea and nitrite react in acid solution and generate the nitrogen effusion, Griess reagent can generate the aubergine dyestuff with the reaction of nitrite generation azo, the cow's milk that mixes urea can not develop the color owing to the consumption of nitrite, normal milk is displaing amaranth then, and the urea content that aubergine is mixed in the bright raw milk of elementary introduction more is many more.
3. dietary alkali test experience: its experimental principle is the bromothymol blue indicator, can color be changed by Huang Zhilan in the alkaline solution of pH6.0-7.6.If mix alkaline matter (as sodium carbonate, sodium bicarbonate etc.) in the milk.PH changes, and causes that therefore bromothymol blue shows different change color, and the dietary alkali that mixes in the blue dark more explanation raw milk is many more.
4. salt test experience: experimental principle is silver nitrate and potassium chromate effect, generates brown siliver chromate, and siliver chromate can generate white silver chloride and yellow sodium chromate with the sodium chloride reaction.Therefore the normal newborn nondiscolouring dairy products that are mixed with salt then become yellow by peony, and the salt that is mixed in the yellow dark more explanation raw milk is many more.
The milk quick detection test paper bar of mingling of the present invention is formed a packing by four kinds of test strips that detect starch, urea, alkali, salt respectively, can detect four kinds simultaneously during use and mingle material.Wherein, the manufacture craft of starch test strip is with I
2Concentration be that the concentration of 0.032mol/mL, KI is that the Wagner's reagent of 0.126mol/mL respectively takes out behind the 10ml mixing 1 times of dilution, the quantitative test paper of not cutting is fully immersed in the disk that fills the Wagner's reagent that diluted, after soaking 5min its place that is positioned over 5～6 ℃ of lucifuges is dried, with the test paper cutting, and place the lucifuge place to preserve after drying; The manufacture craft of carbamide detection test paper bar is to get 0.04% sodium nitrite hydrochloric acid solution 6ml, in solution, add 0.4g Griess reagent mixing, the color of solution is royal purple behind the mixing, quantitative test paper is soaked in the mixed solution to soak to take out behind the 10min dries in the cool, treat test paper dry after with the test paper cutting, and place the lucifuge place to preserve; The manufacture craft of dietary alkali test strip is that the ethanolic solution of 0.04% bromothymol blue that will configure takes out 10ml and is positioned in the disk, be soaked in the quantitative test paper of not cutting in the reagent fully, after soaking 10～15min the test paper taking-up is dried, treat test paper dry fully after with the test paper cutting, place in the dry box and deposit; The manufacture craft of salt test strip is that the potassium chromate solution with the liquor argenti nitratis ophthalmicus 15ml of 0.01mol/mL and 6 10% mixes rapidly, and the quantitative test paper rapid soaking of not cutting is in mixed solution, soak time is controlled at about 10min, take out after immersion finishes and dry in the place of natural ventilation, the test paper cutting that to dry at last is positioned in the dry box and preserves.
Above-mentioned test strips can also be made into other carriers or forms such as cloth, cotton swab, reagent bottle or reaction small test tube according to its principle.
Four kinds of test strips are placed in the box of drying, lucifuge and preserve, during use, take out these four kinds of test strips respectively and just can carry out the scene and detect, judge wherein whether have starch, urea, alkali, salt raw milk.Therefore note during use,, produce and not directly touch test strip when using the salt test strip, should take to wear safeguard procedures such as gloves with hand because of potassium chromate has carcinogenicity.
The present invention compared with prior art, as can be known from the above technical solutions, the milk quick detection test paper bar of mingling of the present invention has following characteristics: the present invention will detect various milk and mingle the experiment of material and be transformed on the test strips, the needs that adapt to dairy products manufacturer, emphasis detects simultaneously to materials such as the starch that often mixes in the raw material milk, urea, alkali, salt, realize the quick online detection of raw material milk, guarantee dairy food quality.Have easy to carry, simple to operate, detect rapidly, (accuracy of starch test strip is 87% to the accuracy of measurement height, the accuracy of carbamide detection test paper bar is 87%, the accuracy of dietary alkali test strip is 95.7, the accuracy of salt test strip is 90.3%), it is highly sensitive that (sensitivity of starch test strip is 100%, the sensitivity of carbamide detection test paper bar is 94.7%, the sensitivity of dietary alkali test strip is 100%, the sensitivity of salt test strip is 92%), detectability is low, and (lowest detection of starch test strip is limited to 0.07mg/mL, and the lowest detection of carbamide detection test paper bar is limited to 0.16mg/mL, the lowest detection of dietary alkali test strip is limited to 0.006mg/mL, the lowest detection of salt test strip is limited to 0.008mg/mL) etc. characteristics, can carry out the scene to raw milk to detect the detection sensitivity height, testing result is accurate, hold first of Dairy Production and close, shortened the time of Dairy Production, Dairy Production is had certain practical significance.
Description of drawings
Fig. 1 is that starch test strip lowest detectable limit is measured curve map;
Fig. 2 is starch test strip color developing effect figure;
Fig. 3 is that carbamide detection test paper bar lowest detectable limit is measured curve map;
Fig. 4 is carbamide detection test paper bar color developing effect figure;
Fig. 5 is that dietary alkali test strip lowest detectable limit is measured curve map;
Fig. 6 is the dietary alkali test strip;
Fig. 7 is dietary alkali test strip color developing effect figure;
Fig. 8 is that salt test strip lowest detectable limit is measured curve map;
Fig. 9 is salt test strip color developing effect figure.
Embodiment
Below in conjunction with embodiment, a kind of milk quick detection test paper bar of mingling that foundation the present invention is proposed is described in detail as follows:
The present invention proposes following explained hereafter flow process by experiment:
(1) starch test strip: 1. test strips is made: with I
2Concentration be that the concentration of 0.032mol/mL, KI is that the Wagner's reagent of 0.126mol/mL respectively takes out behind the 10ml mixing 1 times of dilution.The quantitative test paper of not cutting is fully immersed in the disk that fills the Wagner's reagent that diluted, behind the immersion 5min its place that is positioned over lucifuge 5-6 ℃ is dried.After drying test paper is cut to the test strips of long 3cm, wide 1cm, and places the lucifuge place to preserve.
2. the test strips lowest detectable limit is measured: prepare 10 test tubes and number from 1-10 respectively, in each test tube, add the 5ml raw milk respectively, No. 1 pipe is as blank, the starch that adds different amounts in all the other test tubes respectively, be positioned on the spirit lamp and heat slightly adding raw milk behind the starch after stirring, pick inferior slightly milk with glass bar after cooling and drip its change color of observation on the test strips of making.("-" expression does not have color reaction, and "+" expression has blue reaction)
Table 1. starch test strip lowest detectable limit is measured
Starch test strip lowest detectable limit measurement result such as Fig. 1
Above experimental data is three experiment gained data mean values, can find out that from data presentation the lowest detection of this quick starch test strip is limited to 0.07mg/mL.
3. the half-quantitative detection scope is definite: get 20 test tubes labels respectively, inject the 5ml raw milk respectively in each test tube, No. 1 pipe adds the different starch of measuring respectively as the blank pipe in all the other pipes, be positioned on the spirit lamp after mixing and heat slightly.The sample of waiting to suckle cooling back picks a little with glass bar and drops in its change color of observation on the test strips of making.
Table 2 starch test strip semiquantitative determination
Above experimental data is three repeated experiments gained data mean values, from above-mentioned data can find out this starch quick detection test paper bar content of starch for 0.07mg/mL-0.75mg/mL time colour developing for light blue, content of starch for 0.92mg/mL-1.77mg/mL time colour developing for blue, time colour developing is darker blueness for 1.94mg/mL-2.62mg/mL at content of starch, content of starch during for 2.79mg/mL-3.50mg/mL colour developing be mazarine.
4. the mensuration of test strips recall rate: get 15 test tubes labels respectively, inject the 5ml raw milk respectively from 1-15, and the starch that adding difference is respectively measured in raw milk, the sample of will suckling behind the mixing heats on spirit lamp slightly, sample to be suckled picks a little milk sample dropping on the test strips made from glass bar after being chilled to room temperature, observes its change color.
Table 3 starch quick detection test paper bar recall rate is measured
Above experimental data is the mean value of gained data after the repeated experiments three times, have above data can show the demonstration of each starch test strip remove 10, No. 14 pipes show wrong all the other all in the scope of half half-quantitative detection, from then on can find out that this starch quick detection test paper bar recall rate reaches 100%, accuracy reaches 87%.
5. the term of validity of starch test strip is measured: the test strips of made is divided into 4 groups, self-control is finished and was counted the same day, took out one group of test strips in per 10 days and do and detect the test of starch milk sample, measure it and preserve the term of validity, and detect salt simultaneously, whether white sugar influential to its testing result.
The table 4. starch quick detection test paper bar pot-life is measured
Through experiment, adding salt, white sugar etc. in the dairy products does not all influence its detection effect.
Starch test strip color developing effect is seen Fig. 2, and content of starch successively decreases in the raw milk from left to right.
(2) carbamide detection test paper bar:
1. the making of test strips: get 0.04% sodium nitrite hydrochloric acid solution 6ml, add 0.4g Griess reagent mixing in solution, the color of solution is royal purple behind the mixing.Quantitative test paper is soaked in the mixed solution to soak to take out behind the 10min dries in the cool.Treat after test paper dries test paper to be cut to the test strips of long 3.5cm, wide 1.5cm, and place the lucifuge place to preserve.
2. the mensuration of test strips lowest detectable limit: get 10 test tubes labels respectively, in each test tube, inject the 5ml raw milk, No. 1 test tube is as blank, all the other test tubes are put into the urea of different amounts respectively, shake up the back and pick a little dropping on the test strips of making, observe the time that its change color and record reflection produce with glass bar.
Table 5. carbamide detection test paper bar lowest detectable limit is measured
Carbamide detection test paper bar lowest detectable limit measurement result such as Fig. 3.
The above-mentioned experiment of triplicate, the lowest detection of the test strip of this experiment of averaging of income data presentation made is limited to 0.16mg/mL, and the time of colour developing is 4min.
3. the mensuration of test strips half-quantitative detection: get 20 test tubes labels respectively, in each test tube, inject the 5ml raw milk, No. 1 pipe is as blank, all add different urea in all the other test tubes and shake up the back and pick a little with glass bar and drip on the test strips of making, observe the time that its change color and record reflection produce.
The half-quantitative detection of table 6. carbamide detection test paper bar
The above-mentioned experiment of triplicate, the half-quantitative detection scope of the test strip of this experiment of averaging of income data presentation made is 0.16mg/mL-2.5mg/mL.The colour developing of blank is a royal purple, carbamide detection test paper bar mensuration urea content is that the milk sample colour developing of 0.16mg/mL-0.78mg/mL is aubergine, urea content is that the milk sample colour developing of 0.78mg/mL-1.56mg/mL is light violet magenta, urea content is that the milk sample colour developing of 1.56mg/mL-2.17mg/mL is pink, and urea content is that the milk sample colour developing of 2.17mg/mL-2.50mg/mL is milky.
4. the mensuration of test strips recall rate: get 15 test tubes, number respectively, in every test tube, inject the 5ml raw milk respectively and add the different urea of measuring, pick a little milk sample with glass bar after shaking up and drip on the test strips of making, observe its change color to every test tube.
Table 7. carbamide detection test paper bar recall rate is measured
The above-mentioned experiment of triplicate, the recall rate of this urea quick detection test paper bar of averaging of income data presentation is 94.7%, accuracy is 87%.
5. the carbamide detection test paper bar term of validity is measured: the test strips of made is divided into 4 groups, self-control is finished and was counted the same day, take out one group of test strips in per 10 days and do the test of detection urea milk sample, measure it and preserve the term of validity, and detection simultaneously thinks except that urea whether other materials detect effect to it influential.The result shows that the 4th group test strips testing result is undesirable, the recall rate of first group of test strips (10 days) is 93.2%, accuracy 87%, the recall rate of second group of test strips (20 days) is 89.3%, accuracy 85.7%, the recall rate of the 3rd group of test strips (30 days) is 77.3%, and accuracy is 80.1%.And experiment shows that other materials do not have influence to experiment effect.
The table 8. carbamide detection test paper bar term of validity is measured
Carbamide detection test paper bar color developing effect such as Fig. 4, the content of urea in the raw milk reduces gradually from left to right.
(3) dietary alkali test strip
1. the making of test strips: the 0.04% bromothymol blue ethanol indicator that will configure takes out 10ml and is positioned in the disk, be soaked in the quantitative test paper of not cutting in the reagent fully, after soaking 10-15min the test paper taking-up is dried, treat after test paper dries fully test paper to be cut to long 3cm, the test strips of wide 1cm places in the dry box and deposits.
2. the mensuration of test strips lowest detectable limit: get 10 test tubes and number respectively, inject the 5ml raw milk in every test tube, No. 1 pipe adds the sodium bicarbonate of different amounts respectively as blank in all the other test tubes.Shake up the back and pick a little milk sample dropping on the test strips of making, observe its change color, write down the sodium bicarbonate consumption and the reaction time of each test tube with glass bar.
The mensuration of table 9. dietary alkali test strip lowest detectable limit
(the blue color depth of "+" expression)
Dietary alkali test strip lowest detectable limit is measured, result such as Fig. 5
The above-mentioned experiment of triplicate, the lowest detection of the dietary alkali test strip of this experiment of averaging of income data presentation made is limited to 0.006mg/mL, and developing time is 5S.
3. the mensuration of test strips half-quantitative detection: get 20 test tubes and number respectively, inject the 5ml raw milk in every test tube, No. 1 pipe adds the sodium bicarbonate of different amounts respectively as blank in all the other test tubes.Shake up the back and pick a little milk sample dropping on the test strips of making, observe its change color, write down the sodium bicarbonate consumption and the reaction time of each test tube with glass bar.
Table 10. dietary alkali test strip half-quantitative detection
The above-mentioned experiment of triplicate, the mean value of gained data shows that the half-quantitative detection scope of the dietary alkali test strip that this experiment is researched and developed is, the colour developing of the content of sodium bicarbonate test strips when 0.006mg/mL-0.024mg/mL is light blue, the colour developing of content test strips when 0.024mg/mL-0.058mg/mL is light blue, the colour developing of content test strips when 0.058mg/mL-0.104mg/mL is blue, and the colour developing of content test strips when 0.104mg/mL-0.15mg/mL is a mazarine.
4. the mensuration of test strips recall rate: get 15 test tubes, number respectively, in every test tube, inject the 5ml raw milk respectively and add the different sodium bicarbonate of measuring, pick a little milk sample with glass bar after shaking up and drip on the test strips of making, observe its change color to every test tube.
Table 11. dietary alkali test strip recall rate is measured
The above-mentioned experiment of triplicate, the mean value of the data of gained show that the recall rate of the dietary alkali quick detection test paper bar that this experiment is developed is 100%, and accuracy is 95.7%.
5. the dietary alkali test strip term of validity is measured: the test strips of made is divided into 4 groups, self-control is finished and was counted the same day, take out one group of test strips in per 10 days and do the test of detection sodium bicarbonate milk sample, measure it and preserve the term of validity, and whether other materials that detect simultaneously except that sodium bicarbonate are influential to its detection effect.
The table 12. dietary alkali test strip term of validity is measured
Experimental result shows that the detection term of validity of this dietary alkali test strip is 30-40 days.And other materials do not have influence to experimental result.
Dietary alkali test strip such as Fig. 6, dietary alkali test strip color developing effect such as Fig. 7, the dietary alkali content in the raw milk successively decreases from left to right.
(4) salt test strip
1. the making of test strips: the liquor argenti nitratis ophthalmicus 15ml of 0.01mol/mL and 6 10% potassium chromate solution are mixed rapidly, and the quantitative test paper rapid soaking of not cutting is in mixed solution, soak time is controlled at about 10min, soaks the back taking-up that finishes and dries in the place of natural ventilation.The test paper that will dry is cut to long 3.5cm at last, and the test strips of wide 1.5cm is positioned in the dry box and preserves.
2. the mensuration of test strips lowest detectable limit: get 10 test tubes and number respectively, in every test tube, inject the raw milk of 5ml, No. 1 pipe is as blank, put into the salt of different amounts in all the other test tubes respectively, mix the back and pick a little milk sample dropping on the test strips of making, observe its change color, if the test strips flavescence illustrates that then there is salt the side in the milk sample with glass bar, salt content when experimental record produces change color in the milk sample to be measured, and record produces the required time of change color.
Table 13. salt test strip lowest detectable limit is measured
Salt test strip lowest detectable limit measurement result such as Fig. 8.
The above-mentioned experiment of triplicate can draw from the mean value of gained data, and the lowest detection of the salt test strip that this experiment is developed is limited to 0.008mg/mL, and the required time is 1.5min.
3. the mensuration of test strips half-quantitative detection: get 20 test tubes and number respectively, to inject the 5ml raw milk in every test tube, No. 1 pipe adds the salt of different amounts respectively as blank in all the other test tubes.Shake up the back and pick a little milk sample dropping on the test strips of making, observe its change color, write down the salt content and the reaction time of each test tube with glass bar.
Table 14. salt test strip semiquantitative determination
The above-mentioned experiment of triplicate, the mean value of gained data shows that the half-quantitative detection scope of the salt test strip that this experiment is researched and developed is, salt content test strips colour developing when 0.008mg/mL-0.191mg/mL is bright yellow, content test strips colour developing when 0.191mg/mL-0.310mg/mL is faint yellow, the test strips colour developing is for light yellow when 0.310mg/mL-0.475mg/mL for content, and content test strips colour developing when 0.475mg/mL-0.76mg/mL is yellow.
4. the mensuration of test strips verification and measurement ratio: get 15 test tubes, number respectively, in every test tube, inject the 5ml raw milk respectively and add the different salt of measuring, pick a little milk sample with glass bar after shaking up and drip on the test strips of making, observe its change color to every test tube.
Table 15. salt test strip recall rate is measured
The above-mentioned experiment of triplicate, the mean value of the data of gained show that the recall rate of the salt quick detection test paper bar that this experiment is developed is 92%, and accuracy is 90.3%.
5. the salt test strip term of validity is measured: the test strips of made is divided into 4 groups, self-control is finished and was counted the same day, take out one group of test strips in per 5 days and do the test of detection sodium bicarbonate milk sample, measure it and preserve the term of validity, and whether detect other materials of thinking except that salt simultaneously influential to its detection effect.
The table 16. salt test strip term of validity is measured
Experimental result shows that the detection term of validity of this salt test strip is 15-20 days.And other materials do not have influence to experimental result.
Salt test strip color developing effect such as Fig. 9, rightmost is blank, all the other pictures size of experiment in the raw milk from left to right successively decrease.
The milk quick detection test paper bar of mingling of the present invention has following characteristics:
It is (1) of the present invention that to mingle milk quick detection test paper bar easy and simple to handle, the accuracy in detection height, and the scene that can be used for multiple actual production scene is detected, and is time saving and energy saving.
(2) the present invention is that four kinds of test strips are formed a packing, guarantees to detect simultaneously 4 kinds of adulterants.
(3) four kinds of quick detection test paper bars of the present invention are to invent at several materials of mingling common on the present market, have practical value.
(4) of the present invention to mingle milk quick detection test paper bar detectability low, detection sensitivity height, accuracy height.
(5) the milk quick detection test paper bar of mingling of the present invention can be made simple half-quantitative detection to the adulterant mass-energy in the milk sample to be measured.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, any technical solution of the present invention content that do not break away from,, all still belong in the scope of technical solution of the present invention any simple modification, equivalent variations and modification that above embodiment did according to technical spirit of the present invention.
Claims (3)
1. mingle milk quick detection test paper bar for one kind, be made up of four kinds of test strips that detect starch, urea, alkali, salt respectively, it is characterized in that: the manufacture craft of starch test strip is with I
2Concentration be that the concentration of 0.032mol/mL, KI is that the Wagner's reagent of 0.126mol/mL respectively takes out behind the 10ml mixing 1 times of dilution, the quantitative test paper of not cutting is fully immersed in the disk that fills the Wagner's reagent that diluted, after soaking 5min its place that is positioned over lucifuge 5-6 ℃ is dried, with the test paper cutting, and place the lucifuge place to preserve after drying; The manufacture craft of carbamide detection test paper bar is to get 0.04% sodium nitrite hydrochloric acid solution 6ml, in solution, add 0.4g Griess reagent mixing, the color of solution is royal purple behind the mixing, quantitative test paper is soaked in the mixed solution to soak to take out behind the 10min dries in the cool, treat test paper dry after with the test paper cutting, and place the lucifuge place to preserve; The manufacture craft of dietary alkali test strip is that the ethanolic solution of 0.04% bromothymol blue that will configure takes out 10ml and is positioned in the disk, be soaked in the quantitative test paper of not cutting in the reagent fully, after soaking 10-15min the test paper taking-up is dried, treat test paper dry fully after with the test paper cutting, place in the dry box and deposit; The manufacture craft of salt test strip is that the potassium chromate solution with the liquor argenti nitratis ophthalmicus 15ml of 0.01mol/mL and 6 10% mixes rapidly, and the quantitative test paper rapid soaking of not cutting is in mixed solution, soak time is controlled at about 10min, take out after immersion finishes and dry in the place of natural ventilation, the test paper cutting that to dry at last is positioned in the dry box and preserves.
2. the milk quick detection test paper bar of mingling as claimed in claim 1 is characterized in that: test paper can also be cloth, cotton swab, reagent bottle or reaction small test tube.
3. the milk quick detection test paper bar of mingling as claimed in claim 1 or 2 is characterized in that: four kinds of test strips are formed a packing, guarantee to detect simultaneously four kinds of adulterants.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100905325A CN102253037A (en) | 2011-04-12 | 2011-04-12 | Test paper slip for testing adulterated milk rapidly |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100905325A CN102253037A (en) | 2011-04-12 | 2011-04-12 | Test paper slip for testing adulterated milk rapidly |
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| CN102253037A true CN102253037A (en) | 2011-11-23 |
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| CN2011100905325A Pending CN102253037A (en) | 2011-04-12 | 2011-04-12 | Test paper slip for testing adulterated milk rapidly |
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| CN104280387A (en) * | 2014-09-20 | 2015-01-14 | 中山鼎晟生物科技有限公司 | Kit and method for rapidly detecting urea in milk product |
| CN104849270A (en) * | 2015-05-22 | 2015-08-19 | 汇征联合（北京）医疗器械有限公司 | Reagent for determining sodium chloride content in urine, test paper strip thereof, preparation method thereof and purpose thereof |
| CN108872224A (en) * | 2018-07-13 | 2018-11-23 | 云南商测质量检验技术服务有限公司 | A kind of Fast Determination of Pesticide Residue ball and preparation method thereof |
| WO2023180910A1 (en) * | 2022-03-21 | 2023-09-28 | M-Lense Research Private Limited | A paper-based rapid test card for the detection of adulterants in milk |
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|---|---|---|---|---|
| CN104280387A (en) * | 2014-09-20 | 2015-01-14 | 中山鼎晟生物科技有限公司 | Kit and method for rapidly detecting urea in milk product |
| CN104849270A (en) * | 2015-05-22 | 2015-08-19 | 汇征联合（北京）医疗器械有限公司 | Reagent for determining sodium chloride content in urine, test paper strip thereof, preparation method thereof and purpose thereof |
| CN108872224A (en) * | 2018-07-13 | 2018-11-23 | 云南商测质量检验技术服务有限公司 | A kind of Fast Determination of Pesticide Residue ball and preparation method thereof |
| WO2023180910A1 (en) * | 2022-03-21 | 2023-09-28 | M-Lense Research Private Limited | A paper-based rapid test card for the detection of adulterants in milk |
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Application publication date: 20111123 |