CN102323258A - Detection test paper for detecting benzoyl peroxide in flour and preparation method of detection test paper - Google Patents
Detection test paper for detecting benzoyl peroxide in flour and preparation method of detection test paper Download PDFInfo
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- CN102323258A CN102323258A CN201110162397A CN201110162397A CN102323258A CN 102323258 A CN102323258 A CN 102323258A CN 201110162397 A CN201110162397 A CN 201110162397A CN 201110162397 A CN201110162397 A CN 201110162397A CN 102323258 A CN102323258 A CN 102323258A
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Abstract
The invention discloses detection test paper for detecting benzoyl peroxide in flour and preparation method of the detection test paper. The test paper comprises a test strip and a standard colourimetric card, wherein the standard colourimetric card comprises 10 color lumps with corresponding concentration gradients of benzoyl peroxide from shallow to deep respectively 0mg/L, 12 mg/L, 36 mg/L, 60 mg/L, 84 mg/L, 108 mg/L, 132 mg/L, 156 mg/L, 180 mg/L and 228 mg/L. The test strip comprises a substrate and a color-developing layer adhered on the front end of the substrate, the color-developing layer has a test function, the substrate is made of a PVC (polyvinyl chloride) sheet, and the color-developing layer is prepared by immersing a cellulose acetate film in a color-developing mixed solution consisting of 5-10 percent of potassium iodide, 0.2-0.8 percent of soluble starch and 2-8 percent of sodium carbonate and then drying in vacuum at a temperature of 40-80 DEG C for 20-50min. According to the comparison of the color-developing degree of a sample to be detected and the standard colourimetric card, the content range of the benzoyl peroxide can be rapidly judged. The detection test paper disclosed by the invention is simple, rapid and visual in operation when being used for detecting the benzoyl peroxide remained in the flour, and has high detection sensitivity and accuracy.
Description
Technical field
The present invention relates to a kind of fast, the detection test paper of benzoyl peroxide and residual quantity thereof in the half-quantitative detection flour, can be applicable to primary dcreening operation and the field quick detection and the supervision of benzoyl peroxide in the flour, it belongs to the food security field.
Background technology
(Benzoyl peroxide BPO) has strong oxidation to benzoyl peroxide, and is widely-used by domestic and international flour processing industry as the quality improvement agent and the whitening agent of wheat flour.Benzoyl peroxide content is one of important indicator of flour security, and with the relevant quality of flour relation is closely arranged.After wheat flour stored certain hour, the benzoyl peroxide of adding can impel its rapid aging, hydrolysis but takes place self generate active oxygen and benzoic acid; Pigments such as carotenoid in the active oxygen ability oxidation wheat flour and xenthophylls, thus reach the decolouring whitening effect; Benzoic acid meeting CKIs enzyme and growth of microorganism are avoided protein breakdown, improve the wheaten food storge quality.The consequence of bringing simultaneously is to have destroyed multivitamin nutritive value in the wheat flour, and after benzoic acid got into human body with flour product, the excess intake benzoic acid can influence the liver physiology function of human body, unfavorable health.Flour has self distinctive wheat fragrance, and is little to the smell influence of flour under the situation of normal addition, but excessive interpolation is prone to make flour to lose original wheat fragrance, and especially flour is stored in a humid environment, is easy to generate peculiar smell.In addition, undecomposed benzoyl peroxide forms phenyl ring base [C when heating
6H
5], combine then to form phenol with OH-, have special peculiar smell, toxic.
European Union formally forbade in 1997, and Australia and New Zealand also forbid subsequently.All there is the strict use standard of limiting the quantity of in many countries, and limiting the quantity of of the U.S. is 0.045g/kg.Among China standard GB 2760-1996 in the regulation flour limiting the quantity of of benzoyl peroxide be 0.06g/kg.In October, 2008, flour quality safety upgrade forum call prohibition added the chemical whitening agent such as benzoyl peroxide that flour nutrition and health had no benefit in flour; And required before the unmodified standard of national standard, hope that large and medium-sized key enterprise takes the lead the benzoyl peroxide of stopping using.The analytical approach of benzoyl peroxide has vapor-phase chromatography, liquid phase chromatography and AAS.The spectrophotometric analysis time is long, and liquid chromatography and vapor-phase chromatography are GBs, has the accuracy of detection height; Detectability is low; The advantage of good reproducibility, but also exist the sample pre-treatments complex steps, time-consuming, detecting instrument is expensive, the cycle is long, cost is high, and relies on problem such as professional and technical personnel; Be unfavorable for basic unit's quality testing department as the large tracts of land primary dcreening operation, be unfavorable for field quick detection and supervision, be unfavorable for that also consumption guidance person family is to the identification of the selectivity of flour.
Therefore, a kind of easy, quick, efficient, with low cost detection paper technology is provided, to the supervision of benzoyl peroxide in the flour with to detect be a problem of needing solution badly.
Summary of the invention
In order to overcome above-mentioned deficiency of the prior art, the invention provides a kind ofly detect fast, the test strip and preparation method thereof of benzoyl peroxide in the flour of accuracy in detection and highly sensitive, good stability, easy storing.
The objective of the invention is to realize like this:
A kind of detection test paper that detects benzoyl peroxide in the flour comprises benzoyl peroxide test strip and standard color comparison card;
Said benzoyl peroxide standard color comparison card is made up of 10 color lumps (1), is respectively 0mg/L, 12mg/L, 36mg/L, 60mg/ L, 84mg/ L, 108mg/ L, 132mg/ L, 156mg/ L, 180mg/ L, 228mg/ L from shallow to dark concentration gradient corresponding to benzoyl peroxide;
Test strips by substrate (2) be connected and be positioned at the color developing detection layer (3) that substrate (2) front end has test function with substrate (2) and form.
Described benzoyl peroxide standard color comparison card; Its preparation method is: the standard solution of the benzoyl peroxide of variable concentrations is developed the color on the test paper color development layer; According to the production standard color lump as a result that develops the color; The concentration of standard color block by benzoyl peroxide is arranged in order, pastes, make standard color comparison card.
Described substrate (2) is processed by the PVC thin slice, and substrate length is 6～10cm, and width is 0.3～1cm; Described color developing detection layer (3) is processed by CAM, and the area in colour developing check district is 0.01～1cm
2
Described test strips is sxemiquantitative or qualitative test strips.
The preparation method of described test strip is: color developing detection layer (3) is flooded 2～5min in the developer mixed liquor, behind 40～80 ℃ of vacuum drying 20～50min, obtain.
Described developer mixed liquor consists of: 5%～10% potassium iodide, 0.2%～0.8% soluble starch, 2%～8% sodium carbonate.
Described sodium carbonate is natrium carbonicum calcinatum.
Positive beneficial effect:
1. ultimate principle of the present invention is: benzoyl peroxide and potassium iodide reaction generate iodine and benzoic acid; Add starch solution; Color becomes bluish violet, in the 585nm wavelength absorption maximum is arranged, and in the concentration of benzoyl peroxide at 0 ~ 228mg/ L with interior linear.
2. be dissolved in benzoyl peroxide and the potassium iodide effect in the starch potassium iodide paper in the absolute ethyl alcohol; Produce a certain amount of iodine; Under the condition that with water is solvent, make starch become blue; Through the hepatic depth of starch potassium iodide paper, the standard color comparison card of contrast benzoyl peroxide, the content range of judgement benzoyl peroxide.
3. indicator of the present invention: the present invention selects for use potassium iodide as indicator, when it is formed iodide ion by the benzoyl peroxide oxidation, is reacted into blueness with soluble starch.
When iodine liquid contacts with starch; Iodine molecule can get into the spiral inside of starch molecule; Average per six glucose units (every circle spiral) can fetter an iodine molecule, and whole amylose molecule can fetter a large amount of iodine molecules, and this has just formed the compound of blue starch-iodine.
4. colour developing protective agent of the present invention
The present invention adds sodium carbonate can suppress separating out of iodine effectively, and this is because sodium carbonate hydrolytic reactions in the WS.
Description of drawings
The structural representation of Fig. 1 benzoyl peroxide standard color comparison card;
Fig. 2 test strip structural representation;
Among the figure color lump 1, substrate 2, color developing detection layer 3.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further described:
A kind of detection test paper that detects benzoyl peroxide in the flour comprises test strips and benzoyl peroxide standard color comparison card;
As shown in Figure 1; Said benzoyl peroxide standard color comparison card is made up of 10 color lumps (1), is respectively 0mg/L, 12mg/L, 36mg/L, 60mg/ L, 84mg/ L, 108mg/ L, 132mg/ L, 156mg/ L, 180mg/ L, 228mg/ L from shallow to dark concentration gradient corresponding to benzoyl peroxide;
As shown in Figure 2, test strips by substrate (2) be connected and be positioned at the color developing detection layer (3) that substrate (2) front end has test function with substrate (2) and form.
Described benzoyl peroxide standard color comparison card; Its preparation method is: the standard solution of the benzoyl peroxide of variable concentrations is developed the color on the test paper color development layer; According to the production standard color lump as a result that develops the color; The concentration of standard color block by benzoyl peroxide is arranged in order, pastes, make standard color comparison card.
Described substrate (2) is processed by the PVC thin slice, and substrate length is 6～10cm, and width is 0.3～1cm; Described color developing detection layer (3) is processed by CAM, and the area in colour developing check district is 0.01～1cm
2
Described test strips is sxemiquantitative or qualitative test strips.
The preparation method of described test strip is: color developing detection layer (3) is flooded 2～5min in the developer mixed liquor, behind 40～80 ℃ of vacuum drying 20～50min, obtain.
Described developer mixed liquor consists of: 5%～10% potassium iodide, 0.2%～0.8% soluble starch, 2%～8% sodium carbonate.
Described sodium carbonate is natrium carbonicum calcinatum.
1. the preparation of test paper: the preparation of mixed liquor: 5% potassium iodide, 0.2% soluble starch, 2% sodium carbonate mixes.Get and take out after the good water absorption CAM immerses developer mixed liquor 2min, be placed on the dry 30min of 40 ℃ of lucifuges of vacuum, the dry test paper that obtains is cut into 0.5cm
2Fritter be pasted on the substrate front end.Substrate is processed by the PVC thin slice, and substrate length is 8cm, and width is 0.5cm
2
2. the making of standard color comparison card:
(1) the benzoyl peroxide standard solution of preparation 0mg/L, 12mg/L, 36mg/L, 60mg/ L, 84mg/ L, 108mg/ L, 132mg/ L, 156mg/ L, 180mg/ L, 228mg/ L, the benzoyl peroxide solution of getting variable concentrations carries out chromogenic reaction on the detection test paper color development layer for preparing.
(2) utilize colour photometer to measure test paper L*, a* and b* Value Data, the input computing machine utilizes color module software that color data is simulated, the production standard comparator block.Press the height of the concentration of benzoyl peroxide solution, the standard comparator block is arranged, pasted, process the standard color comparison card of benzoyl peroxide.
(3) detection method of benzoyl peroxide in the flour
Get flour 100g, add 300mL distilled water, after mixing; The centrifugal 5min of 4000rpm; Get supernatant and be added on the above-mentioned test paper color development layer, when the content of the benzoyl peroxide in the flour met or exceeded 36mg/kg, test paper just demonstrated light blue in 10 ~ 30s; The contrast standard colorimetric card draws the content range of benzoyl peroxide.
1. the preparation of test paper: the preparation of mixed liquor: 10% potassium iodide, 0.8% soluble starch, 8% sodium carbonate mixes.Get and take out after the good water absorption CAM immerses developer mixed liquor 5min, be placed on the dry 20min of 50 ℃ of lucifuges of vacuum, the dry test paper that obtains is cut into 0.8cm
2Fritter be pasted on the substrate front end.Substrate is processed by the PVC thin slice, and substrate length is 8cm, and width is 0.8cm
2
2. the making of standard color comparison card:
(1) preparation contains the benzoyl peroxide standard solution of concentration gradient 0mg/L, 12mg/L, 36mg/L, 60mg/ L, 84mg/ L, 108mg/ L, 132mg/ L, 156mg/ L, 180mg/ L, 228mg/ L respectively, and the benzoyl peroxide solution of getting variable concentrations is added on the detection test paper color development layer for preparing and develops the color.
(2) utilize computer A dobe Photoshop to edit the rgb value of different colours; Compile versicolor color lump with rgb value; With the color contrast of the benzoyl peroxide test paper color development of normal concentration,, prepare standard color block again up to editing out the color that is complementary with standard benzoyl peroxide concentration; Press the colour developing of benzoyl peroxide concentration standard and arrange, paste, process the benzoyl peroxide standard color comparison card
(3) detection method of benzoyl peroxide in the flour
Get flour 100g and add 300mL distilled water; After mixing, the centrifugal 5min of 4000rpm gets supernatant and is added on the above-mentioned test paper color development layer; When the content of the benzoyl peroxide in the flour meets or exceeds 36mg/kg; Test paper just demonstrates light bluely in 10 ~ 30s, and the contrast standard colorimetric card draws the content range of benzoyl peroxide.
(1) test paper method:
Get flour 100g and add 300mL distilled water, after mixing, the centrifugal 5min of 4000rpm gets supernatant and is added on a small quantity on the above-mentioned test paper color development layer, and test paper just demonstrates light bluely in 10 ~ 30s, and the contrast standard colorimetric card draws the content range of benzoyl peroxide.
(2) AAS
The preparation of benzoyl peroxide standard solution: the concentration gradient of the standard solution of configuration benzoyl peroxide is respectively: 0mg/L, 30mg/L, 60mg/L, 90mg/ L, 120mg/ L, 150mg/ L, 180mg/ L, the typical curve of drafting benzoyl peroxide.
Get flour 100g and add 300mL distilled water, after mixing, the centrifugal 5min of 4000rpm gets supernatant 5mL, adds the 10%KI of 4.5mL, and the amount of 0.4% starch is 1.5mL, measures absorbance in the 585nm wavelength, calculates the content of benzoyl peroxide.
Utilize test paper method and AAS that the content of the benzoyl peroxide in the flour is measured, measure result's contrast and see table 1.Can find out that from table 1 test paper method and AAS testing result consistance are better, deviation is at zone of reasonableness.Test paper method is convenient, save time, and indicated the content of benzoyl peroxide in the flour preferably.
Table 1 test paper method and AAS detect contrast
Positive beneficial effect:
1. ultimate principle of the present invention is: benzoyl peroxide and potassium iodide reaction generate iodine and benzoic acid; Add starch solution; Color becomes bluish violet, in the 585nm wavelength absorption maximum is arranged, and in the concentration of benzoyl peroxide at 0 ~ 228mg/ L with interior linear.
2. be dissolved in benzoyl peroxide and the potassium iodide effect in the starch potassium iodide paper in the absolute ethyl alcohol; Produce a certain amount of iodine; Under the condition that with water is solvent, make starch become blue; Through the hepatic depth of starch potassium iodide paper, the standard color comparison card of contrast benzoyl peroxide, the content range of judgement benzoyl peroxide.
3. indicator of the present invention: the present invention selects for use potassium iodide as indicator, when it is formed iodide ion by the benzoyl peroxide oxidation, is reacted into blueness with soluble starch.
When iodine liquid contacts with starch; Iodine molecule can get into the spiral inside of starch molecule; Average per six glucose units (every circle spiral) can fetter an iodine molecule, and whole amylose molecule can fetter a large amount of iodine molecules, and this has just formed the compound of blue starch-iodine.
4. colour developing protective agent of the present invention
The present invention adds sodium carbonate can suppress separating out of iodine effectively, and this is because sodium carbonate hydrolytic reactions in the WS.
Above embodiment only is used to explain preferred implementation of the present invention; But the present invention is not limited to above-mentioned embodiment; In the ken that said field those of ordinary skill is possessed; Any modification of being done within spirit of the present invention and the principle, be equal to and substitute and improvement etc., it all should be encompassed within the technical scheme scope that the present invention asks for protection.
Claims (7)
1. a detection test paper that detects benzoyl peroxide in the flour is characterized in that: comprise test strips and benzoyl peroxide standard color comparison card;
Said benzoyl peroxide standard color comparison card has 10 color lumps (1) to form, and is respectively 0mg/L, 12mg/L, 36mg/L, 60mg/ L, 84mg/ L, 108mg/ L, 132mg/ L, 156mg/ L, 180mg/ L, 228mg/ L from shallow to dark concentration gradient corresponding to benzoyl peroxide;
Described test strips by substrate (2) be connected and be positioned at the color developing detection layer (3) that substrate (2) front end has test function with substrate (2) and form.
2. a kind of test strips that detects benzoyl peroxide in the flour according to claim 1 is characterized in that: described substrate (2) is to be processed by the PVC thin slice, and substrate length is 6～10cm, and width is 0.3～1cm; Described color developing detection layer (3) is processed by CAM, and the area in colour developing check district is 0.01～1cm
2
3. a kind of test strips that detects benzoyl peroxide in the flour according to claim 1; It is characterized in that; The preparation method of described test strips is: color developing detection layer (3) is flooded 2～5min in the developer mixed liquor, behind 40～80 ℃ of vacuum drying 20～50min, obtain.
4. a kind of test strips preparation method who detects benzoyl peroxide in the flour according to claim 3 is characterized in that: described developer mixed liquor consists of: 5%～10% potassium iodide, 0.2%～0.8% soluble starch, 2%～8% sodium carbonate.
5. a kind of test paper that detects benzoyl peroxide in the flour according to claim 1; It is characterized in that; The preparation method of described benzoyl peroxide standard color comparison card is: the solution of the benzoyl peroxide of variable concentrations is developed the color on the test paper color development layer, according to colour developing production standard color lump as a result, the concentration gradient of standard color block by benzoyl peroxide is arranged in order; Paste, make standard color comparison card.
6. a kind of test strips that detects benzoyl peroxide in the flour according to claim 1 is characterized in that: described test strips is sxemiquantitative or qualitative test strips.
7. a kind of test strips that detects benzoyl peroxide in the flour according to claim 4 is characterized in that: described sodium carbonate is natrium carbonicum calcinatum.
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| CN 201110162397 CN102323258B (en) | 2011-06-16 | 2011-06-16 | Detection test paper for detecting benzoyl peroxide in flour |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105203534A (en) * | 2015-09-17 | 2015-12-30 | 阜阳师范学院 | Testing identifying method for rapidly measuring content of amylose in flour |
| CN106872451A (en) * | 2016-12-28 | 2017-06-20 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Starch potassium iodide reagents and preparation method thereof, test paper, kit and in flour benzoyl peroxide semi-quantitative detection method |
| CN107688090A (en) * | 2017-07-17 | 2018-02-13 | 润和生物医药科技(汕头)有限公司 | A kind of NTx Test papers, kit and preparation method thereof |
| CN108802021A (en) * | 2018-04-13 | 2018-11-13 | 北京普赞生物技术有限公司 | A kind of benzoyl peroxide Test paper and preparation method thereof and detection method |
| CN113640284A (en) * | 2021-09-06 | 2021-11-12 | 武汉理工大学 | Method and device for rapidly detecting nickel ions |
| CN115028886A (en) * | 2022-06-21 | 2022-09-09 | 贺州学院 | Bisdemethoxycurcumin color development film for detecting lead ions, preparation method thereof and lead ion rapid detection method |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105203534A (en) * | 2015-09-17 | 2015-12-30 | 阜阳师范学院 | Testing identifying method for rapidly measuring content of amylose in flour |
| CN106872451A (en) * | 2016-12-28 | 2017-06-20 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Starch potassium iodide reagents and preparation method thereof, test paper, kit and in flour benzoyl peroxide semi-quantitative detection method |
| CN107688090A (en) * | 2017-07-17 | 2018-02-13 | 润和生物医药科技(汕头)有限公司 | A kind of NTx Test papers, kit and preparation method thereof |
| CN108802021A (en) * | 2018-04-13 | 2018-11-13 | 北京普赞生物技术有限公司 | A kind of benzoyl peroxide Test paper and preparation method thereof and detection method |
| CN108802021B (en) * | 2018-04-13 | 2020-08-04 | 北京普赞生物技术有限公司 | Benzoyl peroxide detection test paper and preparation method and detection method thereof |
| CN113640284A (en) * | 2021-09-06 | 2021-11-12 | 武汉理工大学 | Method and device for rapidly detecting nickel ions |
| CN115028886A (en) * | 2022-06-21 | 2022-09-09 | 贺州学院 | Bisdemethoxycurcumin color development film for detecting lead ions, preparation method thereof and lead ion rapid detection method |
| CN115028886B (en) * | 2022-06-21 | 2023-11-21 | 贺州学院 | Didemethoxycurcumin chromogenic film for detecting lead ions, preparation method thereof and rapid lead ion detection method |
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