CN102520182B - Visible blood glucose test strip and preparation method thereof - Google Patents
Visible blood glucose test strip and preparation method thereof Download PDFInfo
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- CN102520182B CN102520182B CN201110442697.4A CN201110442697A CN102520182B CN 102520182 B CN102520182 B CN 102520182B CN 201110442697 A CN201110442697 A CN 201110442697A CN 102520182 B CN102520182 B CN 102520182B
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Abstract
The invention discloses a visible blood glucose test strip and a preparation method thereof. The visible blood glucose test strip is structurally formed by compounding saturating paper base materials and Millipore nano filter membrane with the bottom face being coated with adhesive. The preparation method comprises the following steps: preparing impregnant solution, preparing the saturating paper base materials, and compounding the saturating paper base materials and the Millipore nano filter membrane. When the test strip is used for blood glucose detection, blood is filtered by the membrane, color development reaction of chromogen reagent, assistant color development reagent and H2O2 is utilized, a special electrochemical signal detection device has no need to be used, so that the effect that blood glucose concentration is quickly and conveniently detected can be achieved. Compared with the test strip with polypropylene, polyethylene, polrvinyl chloride and polycarbonate base materials in the prior art, plant fiber paper base materials of the test strip disclosed by the invention are renewable natural resources, and have the advantages of favorable biodegradation and low price, and the test strip disclosed by the invention can be stored for at least three months at room temperature.
Description
Technical field
The invention belongs to speciality paper product technical field, relate to a kind of blood sugar test paper and preparation method thereof, relate in particular to a kind of visible blood glucose test strip and preparation method thereof.
Background technology
Diabetes are a kind of common metabolic secretion diseases, are one group of clinical syndromes being caused by h and E factor interaction.Due to insulin secretion definitely or relative deficiency, and target tissue cell reduces insulin sensitivity, causes a series of metabolic disorders such as sugar, protein, fat, power and water Xie Zhi.Diabetes can be divided into: type 1 diabetes, diabetes B, Idiotype diabetes and gestational diabetes mellitus.Although the various onset diabetes causes of disease, pathogenesis are different, all take hyperglycaemia as main common trait.
Human normal blood sugar concentration is at 0.60～0.95mg/ml.Fast blood sugar monitoring is very important for diabetic easily.Use that insulin treats diabetic because need to determine insulin dose, need every day the blood sugar concentration of 3～10 times to detect.
In market, there is at present a multiple blood sugar test paper, for example Roche Holding Ag<img TranNum="72" file="GDA0000385233170000011.GIF" he="80" img-content="drawing" img-format="GIF" inline="yes" orientation="portrait" wi="332"/>aviva, Johson & Johnson<img TranNum="73" file="GDA0000385233170000012.GIF" he="78" img-content="drawing" img-format="GIF" inline="yes" orientation="portrait" wi="278"/>ultra2, Abbott Laboratories company limited<img TranNum="74" file="GDA0000385233170000013.GIF" he="78" img-content="drawing" img-format="GIF" inline="yes" orientation="portrait" wi="272"/>freedom, Beyer Co., Ltd<img TranNum="75" file="GDA0000385233170000014.GIF" he="76" img-content="drawing" img-format="GIF" inline="yes" orientation="portrait" wi="252"/>contou test paper, while selecting above-mentioned blood sugar test paper to detect blood sugar concentration, need to be equipped with special electrochemical signals pick-up unit, namely blood glucose meter.Conventionally, before detecting blood sugar concentration, also need blood glucose meter to proofread and correct.Owing to detecting the principle of blood sugar concentration, be electrochemical principle, by detecting the H generating in glucose oxidase process<sub TranNum="76">2</sub>o<sub TranNum="77">2</sub>electrochemical signals determine the concentration of glucose in blood, the base material of test paper is polypropylene, tygon, Polyvinylchloride and polycarbonate plastic, its preparation method need to be on plastic basis material test paper surface layer designing printed circuit.Due to P.e.c. complex manufacturing technology, the blood sugar test paper that the plastics of therefore take are base material has preparation technology and test paper structure is complicated and expensive defect.
Summary of the invention
The object of the invention is to overcome the weak point that prior art exists, and a kind of visible blood glucose test strip is provided.This visible blood glucose test strip is a kind ofly without using special electrochemical signals pick-up unit, only need change by test paper surface color, with naked eyes, judges blood sugar concentration and carries out the quick detection of blood sugar.This visible blood glucose test strip stable performance, inexpensive.
The preparation method who another object of the present invention is to provide a kind of visual blood sugar examination, the method technique is simple, and operation easy to implement, thereby has solved the defect that prior art exists.
For realizing object of the present invention, the technical scheme adopting is:
, it is characterized in that: its structure is scribbled compound composition of Millipore nanofiltration membrane of bonding agent by impregnation processability paper base material and bottom surface, wherein:
Described impregnation processability paper base material, its mixed pulp that optional wherein a kind of paper pulp in bleach chemical pulp, bleached chemi-mechanical pulp and bleached mechanical pulp or optional wherein two kinds of paper pulp mix system with body paper copy system, be quantitatively 20g/m
2～250g/m
2paper;
Described bottom surface scribbles the Millipore nanofiltration membrane of bonding agent, wherein: described bonding agent is 3g/m
2～10g/m
2poly (vinyl alcohol) binder, or 3g/m
2～10g/m
2gelatin adhesive, or 2g/m
2～6g/m
2vinyl acetate binder, optionally wherein a kind of;
Described Millipore nanofiltration membrane ，Wei aperture is less than the Millipore150nm filter membrane of the low protein adsorption of 500nm, or Millipore250nm filter membrane, optionally wherein a kind of.
A kind of preparation method of visible blood glucose test strip, it is characterized in that: preparation method comprises one), the preparation of impregnant solution and the preparation and two of impregnation processability paper base material), that impregnation processability paper base material and bottom surface scribble the Millipore nanofiltration membrane of bonding agent is compound, concrete operations are as follows:
One), the preparation of the preparation of impregnant solution and impregnation processability paper base material
1), the preparation of impregnant solution
1. the component of impregnant solution:
Impregnant solution contains enzyme, chromogen, auxiliary developer, the agent of liquid diffusion control and for regulating the buffer solution of impregnant pH, wherein:
Described enzyme is glucose oxidase and horseradish peroxidase;
Described chromogen, be 2,4, the bromo-3-hydroxybenzoic acid of 6-tri-, 2,4, the bromo-3-hydroxy benzene sulfonic acid of 6-tri-, 2, the bromo-3-hydroxy-benzoic acid of the chloro-6-of 4-bis-, 2, the bromo-3-hydroxy-benzoic acid of the fluoro-6-of 4-bis-, 2, the optional wherein a kind of chromogen in the bromo-3-hydroxyl-benzene sulfonic acid of the chloro-6-of 4-bis-and the bromo-3-hydroxyl-benzene sulfonic acid of the fluoro-6-of 2,4-bis-or the optional wherein chromogen of two kinds of chromogen preparations;
Described auxiliary developer, be 1,5-dimethyl-2-phenyl-4-amino-3-pyrazolone, 1, optionally a kind of auxiliary developer wherein in 5-diethyl-2-phenyl-4-amino-3-pyrazolone and 1-methyl-5-ethyl-2-phenyl-4-amino-3-pyrazolone, or the optional wherein auxiliary developers of two kinds of auxiliary developer preparations;
The agent of described liquid diffusion control, is the optionally wherein a kind of liquid diffusion control agent at the shitosan below 10000, guar gum and gelatin of polyvinyl alcohol (PVA), molecular weight, or the optional wherein liquid diffusion control agent of two kinds of liquid diffusion control agent preparations; The massfraction of described liquid diffusion control agent aqueous solution is 0.2%～10%;
In described impregnant solution, the material proportion of the auxiliary developer of chromogen and auxiliary developer is 1:0.1～3.0, g/g;
In described impregnant solution, the material proportion of chromogen and the agent of liquid diffusion control is: 1:1～10, g/g;
Described for regulating the buffer solution of impregnant pH, for the optionally a kind of buffer solution wherein in citric acid/citric acid, acetic acid/sodium acetate, tartrate/sodium tartrate, malic acid/natrium malicum, or the optional wherein buffer solution of two kinds of buffer preparation;
2. the preparation of impregnant solution
Configure respectively the aqueous solution of aqueous solution, chromogen aqueous solution, auxiliary developer aqueous solution and the agent of liquid diffusion control of enzyme, above solution is mixed, add for regulating the buffer solution of impregnant pH to regulate pH between 3～5, obtain impregnant solution, keep in Dark Place;
The massfraction of described liquid diffusion control agent aqueous solution is 0.2%～10%;
2), the preparation of impregnation processability paper base material
The body paper that impregnation processability paper base material is used cuts into paper slip, getting impregnant solution is contained in a container, paper slip is placed in and in container, immerses impregnant solution, make impregnant solution fully immerse page inside, dip treating temperature range is 3 ℃～60 ℃, and preferred condition is at 15 ℃～40 ℃, soak time is 1.0min～20min, after immersion, take out, dry at 5 ℃～40 ℃; Make impregnation processability paper base material;
Two) it is compound that, impregnation processability paper base material and bottom surface scribble the Millipore nanofiltration membrane of bonding agent
The Millipore nanofiltration membrane that impregnation processability paper base material and bottom surface scribble bonding agent is complex as a whole by the adhesive effect of bonding agent, makes visible blood glucose test strip of the present invention.
] advantage and beneficial effect
Compare with the blood sugar test paper of polypropylene, tygon, Polyvinylchloride and polycarbonate substrate, the advantage of visible blood glucose test strip of the present invention and beneficial effect, as follows respectively:
Advantage
1), in blood sugar test, without use, be equipped with special electrochemical signals pick-up unit, only need change by test paper surface color, with naked eyes, judge blood sugar concentration, there is the effect of quick detection blood sugar.
2), stable performance, at room temperature at least 3 months holding times.
3), inexpensive: approximately 3 yuan～5 yuan/bar of commercially available plastic base blood sugar test paper price, visible blood glucose test strip price of the present invention is only 5% of commercially available plastic base blood sugar test paper price.
Beneficial effect
1), the present invention is usingd plant fiber paper as the body paper of impregnation processability paper base material, is a kind of natural resources of reproducibility, has good biodegradability.
2), compare with plastic basis material, the low price of impregnation processability paper base material, has clear superiority.
3), impregnation processability paper base material has exclusive vegetable fibre structure, is a kind of desirable carrier in glucose detection.By membrane filtration blood, utilize chromogen reagent, help developer and H
2o
2chromogenic reaction, there is the beneficial effect that Quick Measuring goes out blood glucose value.
Accompanying drawing explanation
Fig. 1 is the structural representation of visible blood glucose test strip of the present invention;
Fig. 2 is that after the colour developing of impregnation processability paper base material, its surface reflectivity changes schematic diagram;
Fig. 3 is that after impregnation processability paper base material colour developing, its surface reflectivity changes the relation curve with concentration of glucose (log[C]);
Fig. 4 for dripping respectively the glucose standard solution colour developing of 5.0mL variable concentrations, picture after colour developing on impregnation processability paper base material;
In Fig. 1: 1-Millipore nanofiltration membrane; 2-bonding agent; 3-impregnation processability paper base material.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with accompanying drawing, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
, by impregnation processability paper base material 3 and bottom surface, scribbled compound composition of Millipore nanofiltration membrane 1 of bonding agent 2, wherein:
Described impregnation processability paper base material 3, its mixed pulp that optional wherein a kind of paper pulp in bleach chemical pulp, bleached chemi-mechanical pulp and bleached mechanical pulp or optional wherein two kinds of paper pulp mix system with body paper copy system, be quantitatively 20g/m
2～250g/m
2paper, it is quantitatively 40g/m that the present embodiment is chosen what by bleach chemical pulp, copy system
2paper;
Described bottom surface scribbles the Millipore nanofiltration membrane 1 of bonding agent 2, wherein: described bonding agent 2 is 3g/m
2～10g/m
2poly (vinyl alcohol) binder, or 3g/m
2～10g/m
2gelatin adhesive, or 2g/m
2～6g/m
2vinyl acetate binder, the present embodiment is chosen 3g/m
2poly (vinyl alcohol) binder, described Millipore nanofiltration membrane 1 ，Wei aperture is less than the Millipore150nm filter membrane of the low protein adsorption of 500nm, or Millipore250nm filter membrane, the present embodiment design Millipore150nm filter membrane is Mi Libo (Shanghai) trade Co., Ltd product.
A preparation method for visible blood glucose test strip, comprises one), the preparation of impregnant solution and the preparation and two of impregnation processability paper base material 3), impregnation processability paper base material 3 is compound with the Millipore nanofiltration membrane 1 that scribbles bonding agent 2, concrete operations are as follows:
One), the preparation of the preparation of impregnant solution and impregnation processability paper base material 3
1), the preparation of impregnant solution
1. the component of impregnant solution:
Impregnant solution contains enzyme, chromogen, auxiliary developer, the agent of liquid diffusion control and for regulating the buffer solution of maceration extract pH value, wherein:
Described enzyme is glucose oxidase and peroxidase;
Described chromogen is 2,4, the bromo-3-hydroxybenzoic acid of 6-tri-, 2,4, the bromo-3-hydroxy benzene sulfonic acid of 6-tri-, 2, the bromo-3-hydroxy-benzoic acid of the chloro-6-of 4-bis-, 2, the bromo-3-hydroxy-benzoic acid of the fluoro-6-of 4-bis-, 2, the bromo-3-hydroxyl-benzene sulfonic acid of the chloro-6-of 4-bis-and 2, optional wherein a kind of chromogen in the bromo-3-hydroxyl-benzene sulfonic acid of the fluoro-6-of 4-bis-or the optional wherein chromogen of two kinds of chromogen preparations, the present embodiment is chosen TBHBA and 2, the bromo-3-hydroxy benzene sulfonic acid of 4,6-tri-aqueous solution;
Described auxiliary developer, be 1,5-dimethyl-2-phenyl-4-amino-3-pyrazolone, 1, optionally a kind of auxiliary developer wherein in 5-diethyl-2-phenyl-4-amino-3-pyrazolone and 1-methyl-5-ethyl-2-phenyl-4-amino-3-pyrazolone, or the optional wherein auxiliary developer of two kinds of auxiliary developer preparations, the present embodiment is chosen 1,5-dimethyl-2-phenyl-4-amino-3-pyrazolone aqueous solution;
In described impregnant solution, the material proportion of chromogen and auxiliary developer is 1:0.1～3.0, g/g;
The agent of described liquid diffusion control, for the optionally wherein a kind of liquid diffusion control agent in the shitosan below 10000, guar gum and gelatin of polyvinyl alcohol (PVA), molecular weight, or the optional wherein liquid diffusion control agent of two kinds of liquid diffusion control agent preparations, the present embodiment is chosen polyvinyl alcohol water solution, and the massfraction of described liquid diffusion control agent aqueous solution is 0.2%～10%;
In described impregnant solution, the material proportion of chromogen and the agent of liquid diffusion control is: 1:1～10, g/g;
Described for regulating the buffer solution of maceration extract pH value, for the optionally a kind of buffer solution wherein in citric acid/citric acid, acetic acid/sodium acetate, tartrate/sodium tartrate, malic acid/natrium malicum, or the optional wherein buffer solution of two kinds of buffer preparation, the present embodiment is chosen citric acid/sodium citrate;
2. the preparation of impregnant solution
Measure respectively the 2.5ml enzyme mixed solution that contains 16.2mg glucose oxidase and 1.2mg horseradish peroxidase in the polyvinyl alcohol water solution of 12.0ml5% and every 10ml enzyme mixed solution, and 20.0ml5mg/ml 2,4, the bromo-3-hydroxybenzoic acid of 6-tri-aqueous solution, 1 of 5.0ml20mg/ml, 5-dimethyl-2-phenyl-4-amino-3-pyrazolone aqueous solution, above solution mixed preparing is obtained to impregnant solution, the pH value of modulating impregnant solution with 10.5ml citric acid/sodium citrate buffer solution is between 3-5, standing, keep in Dark Place, stand-by;
2), the preparation of impregnation processability paper base material 3
The body paper of impregnation processability paper base material 3 use is cut to 10 of the paper slips of 4.0cm * 2.5cm specification, getting maceration extract 5ml is contained in a container, paper slip is placed in and in container, immerses impregnant solution, dip treating temperature range is 15 ℃～60 ℃, the present embodiment is designed to 15 ℃, soak time is 1.0min～20min, the present embodiment dip treating temperature design is 5.0min, make impregnant solution fully immerse page inside, after soaking, take out, dry at 5 ℃～40 ℃, the present embodiment baking temperature is designed to 10 ℃, makes impregnation processability paper base material 3;
Two), impregnation processability paper base material 3 is compound with the Millipore nanofiltration membrane 1 that bottom surface scribbles bonding agent 2
The Millipore nanofiltration membrane 1 that impregnation processability paper base material 3 and bottom surface scribble bonding agent 2 is complex as a whole by the adhesive effect of bonding agent, makes visible blood glucose test strip of the present invention.
Described Millipore nanofiltration membrane 1 is Mi Libo (Shanghai) trade Co., Ltd product.
By described same steps as, repeat embodiment 1, just difference is the liquid diffusion control agent concentration of using in impregnant solution preparation, buffer solution and the consumption of enzyme dosage and adjusting maceration extract pH:
The agent of liquid diffusion control is the aqueous guar glue solution of 2.0ml3%;
1.5ml enzyme (contains 16.2mg glucose oxidase and 1.2mg horseradish peroxidase in every 10ml enzyme mixed solution;
The buffer solution that regulates impregnant pH value of solution is 11.5ml citric acid/sodium citrate buffer solution.
By described same steps as, repeat embodiment 2, just difference is buffer solution and the consumption of the adjusting impregnant pH value of solution used in impregnant solution preparation:
The buffer solution that regulates impregnant pH value of solution is 11.5ml malic acid/natrium malicum buffer solution.
Embodiment 4
By described same steps as, repeat embodiment 1, just difference is the liquid diffusion control agent concentration of using in impregnant solution preparation, the buffer solution of enzyme dosage and adjusting impregnant pH value of solution
The agent of liquid diffusion control is the polyvinyl alcohol water solution of 12.0ml6%;
1.5ml enzyme (containing 16.2mg glucose oxidase and 1.2mg horseradish peroxidase in every 10ml enzyme mixed solution), the buffer solution of adjusting impregnant pH value of solution, is 11.5ml acetic acid/sodium acetate buffer solution.
Saturating paper substrate surface Show Color performance of the present invention:
1), the mensuration of Show Color performance
Adopt Datacplor ElREPHO4230 spectrophotometric color measurement instrument to measure the reflectivity on impregnation processability paper base material 3 surfaces, observe visible blood glucose test strip surface Show Color performance change, concrete operations are as follows:
On dried impregnation processability paper base material 3, dripping respectively concentration is 0.72,1.80, the glucose solution of the 5 μ l of 6.30mg/ml, after colour developing 1min, with Datacolor ELREPHO4230 spectrophotometric color measurement instrument, measure the reflectivity on impregnation processability paper base material 3 surfaces, the variation of reflectivity before and after impregnation processability paper base material 3 shows, as shown in Figure 1.
Impregnation processability paper base material 3 shows that back reflection rates change the relation curve with concentration of glucose (log[C]), as shown in Figure 2.
2), after impregnation processability paper base material 3 colour developing, the stability of change color response
Get the impregnation processability paper base material 3 that embodiment 4 obtains, drip the glucose solution colour developing of the 4.5mg/ml of 5.0mL, with Datacolor ELREPHO4230 spectrophotometric color measurement instrument, measure its surperficial reflectance varies, the affect result of response time on impregnation processability paper base material 3 colour developing rear surface reflectance varies, as shown in table 1.As can be seen from Table 1, impregnation processability paper base material 3 is between colour developing 1min～10min, and change color response is stable.
Table 1
| Response time | ΔR | Response time | ΔR |
| 30second | -13.29 | 20min | -18.35 |
| 1min | -18.33 | 40min | -18.36 |
| 2min | -18.33 | 60min | -18.38 |
| 3min | -18.33 | 80min | -18.42 |
| 10min | -18.33 | 100min | -18.45 |
3), test paper stability
Get the impregnation processability paper base material 3 ，Bing Yu bottom surfaces that embodiment 4 obtains and scribble 10g/m
2the Millipore150nm filter membrane 1 of gelatin is compound, obtains test paper, preserve, and by standard glucose solution (4.5mg/ml) Test paper performance, testing result is as shown in table 2 under different condition.As can be seen from Table 2, gained test paper has good stability, at room temperature can preserve at least 3 months.
Table 2.
4), impregnation processability paper base material Show Color effect
Get the impregnation processability paper base material 3 that embodiment 4 obtains, drip respectively the glucose standard solution colour developing of 5.0mL variable concentrations thereon, picture after colour developing, is shown in Fig. 4.
The principle of visible blood glucose test strip of the present invention and application thereof
Principle:
The principle of visible blood glucose test strip test blood sugar of the present invention is by filter membrane 1 filtering blood, utilizes chromogen reagent, helps developer and H
2o
2chromogenic reaction, its course of reaction is as follows:
Application:
In adopting the process of test paper test blood sugar of the present invention, test paper surface demonstrates deep mixed red color blood concentration and indicates, and each red color differing from shallow to deep indicates the height extent value that shows tested blood sugar concentration.
The diabetes that the hyperglycaemia of take is principal character, its incidence of disease is remarkable ascendant trend in recent years, and the whole world approximately has 10% adult to suffer from this disease at present.Nearly 9,000 ten thousand people of 2011 Nian， China diabetic, wherein 18 years old and above resident's diabetes prevalence is about 9.7% ，Er China 18 years old and above resident's diabetes awareness is only 36.1%.China diabetes patient also increases in the speed with annual 1.5%.The visible blood glucose test strip of the blood sugar test of a kind of cheap, easy to use, the dependable performance of the present invention exploitation, for China of diabetes high incidence, detects in diabetes, and diagnosis and therapeutic process have important practical significance.
According to ADA statistics, it is that the sales volume to 2009 year of main all Related products reaches 97.32 hundred million dollars that diabetes test articles for use be take in the U.S., approximately increases progressively 11.2% every year.The total sales volume of the blood sugar test articles for use of China in 2010 surpasses 500,000,000 dollars, and therefore visible blood glucose test strip of the present invention, can be China's diabetes test articles for use market and create considerable economic benefit.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of design concept of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. a visible blood glucose test strip, is characterized in that: its structure is scribbled compound composition of Millipore nanofiltration membrane (1) of bonding agent (2) by impregnation processability paper base material (3) and bottom surface, wherein:
Described impregnation processability paper base material (3), the mixed pulp that the optional wherein a kind of paper pulp of its body paper in bleach chemical pulp, bleached chemi-mechanical pulp and bleached mechanical pulp or optional wherein two kinds of paper pulp mix system copy system, be quantitatively 20g/m
2～250g/m
2paper;
Described bottom surface scribbles the Millipore nanofiltration membrane (1) of bonding agent (2), wherein: described bonding agent (2) is 3～10g/m
2poly (vinyl alcohol) binder, or 3～10g/m
2gelatin adhesive, or 2g/m
2～6g/m
2vinyl acetate binder, optionally wherein a kind of;
Described Millipore nanofiltration membrane (1) ，Wei aperture is less than the Millipore150nm filter membrane of the low protein adsorption of 500nm, or Millipore250nm filter membrane, optionally wherein a kind of.
2. for realizing the preparation method of a kind of visible blood glucose test strip described in claim 1, it is characterized in that: preparation method comprises one), the preparation of impregnant solution and the preparation and two of impregnation processability paper base material (3)), that impregnation processability paper base material (3) ,Yu bottom surface scribbles the Millipore nanofiltration membrane (1) of bonding agent (2) is compound, concrete operations are as follows:
One), the preparation of the preparation of impregnant solution and impregnation processability paper base material (3)
1), the preparation of impregnant solution
1. the component of impregnant solution:
Impregnant solution contains enzyme, chromogen, auxiliary developer, the agent of liquid diffusion control and for regulating the buffer solution of impregnant pH, wherein:
Described enzyme is glucose oxidase and horseradish peroxidase;
Described chromogen, be 2,4, the bromo-3-hydroxybenzoic acid of 6-tri-, 2,4, the bromo-3-hydroxy benzene sulfonic acid of 6-tri-, 2, the bromo-3-hydroxy-benzoic acid of the chloro-6-of 4-bis-, 2, the bromo-3-hydroxy-benzoic acid of the fluoro-6-of 4-bis-, 2, the optional wherein a kind of chromogen in the bromo-3-hydroxyl-benzene sulfonic acid of the chloro-6-of 4-bis-and the bromo-3-hydroxyl-benzene sulfonic acid of the fluoro-6-of 2,4-bis-or the optional wherein chromogen of two kinds of chromogen preparations;
Described auxiliary developer, be 1,5-dimethyl-2-phenyl-4-amino-3-pyrazolone, 1, optionally a kind of auxiliary developer wherein in 5-diethyl-2-phenyl-4-amino-3-pyrazolone and 1-methyl-5-ethyl-2-phenyl-4-amino-3-pyrazolone, or the optional wherein auxiliary developers of two kinds of auxiliary developer preparations;
The agent of described liquid diffusion control, for the optionally wherein a kind of liquid diffusion control agent in the shitosan below 10000, guar gum and gelatin of polyvinyl alcohol (PVA), molecular weight, or the optional wherein liquid diffusion control agent of two kinds of liquid diffusion control agent preparations, the massfraction of described liquid diffusion control agent aqueous solution is 0.2%～10%;
In described impregnant solution, the material proportion of chromogen and auxiliary developer is 1:0.1～3.0, g/g;
In described impregnant solution, the material proportion of chromogen and the agent of liquid diffusion control is: 1:1～10, g/g;
Described for regulating the buffer solution of impregnant pH, for the optionally a kind of buffer solution wherein in citric acid/citric acid, acetic acid/sodium acetate, tartrate/sodium tartrate, malic acid/natrium malicum, or the optional wherein buffer solution of two kinds of buffer preparation;
2. the preparation of impregnant solution
Configure respectively the aqueous solution of aqueous solution, chromogen aqueous solution, auxiliary developer aqueous solution and the agent of liquid diffusion control of enzyme, above solution is mixed, add for regulating the buffer solution of impregnant pH to regulate pH between 3～5, obtain impregnant solution, keep in Dark Place;
2), the preparation of impregnation processability paper base material (3)
The body paper of impregnation processability paper base material (3) use is cut into paper slip, getting maceration extract is contained in a container, paper slip is placed in and in container, immerses maceration extract, dip treating temperature range is 3 ℃～60 ℃, soak time is 1.0min～20min, after immersion, takes out, dry at 5 ℃～40 ℃, make impregnated paper base material (3);
Two), impregnation processability paper base material (3) and bottom surface scribble Millipore filter membrane (1) that the compound impregnation processability paper base material of bonding agent (2) Millipore nanofiltration membrane (1) (3) and bottom surface scribble bonding agent (2) adhesive effect by bonding agent and be complex as a whole, and makes visible blood glucose test strip of the present invention.
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| CN104749171B (en) * | 2013-12-31 | 2018-06-26 | 比亚迪股份有限公司 | Blood sugar detection apparatus and preparation method thereof |
| CN104749169B (en) * | 2013-12-31 | 2018-04-20 | 比亚迪股份有限公司 | Blood sugar detection apparatus and blood sugar test kit |
| CN105353116B (en) * | 2015-11-09 | 2017-07-04 | 国家纳米科学中心 | A kind of method and its application that immunoassay is carried out based on hydrogen peroxide test strips |
| CN111595839A (en) * | 2020-05-19 | 2020-08-28 | 中科康磁医疗科技（苏州）有限公司 | Saliva glucose detection test paper, preparation method and application thereof |
| CN114107436B (en) * | 2021-11-26 | 2024-06-25 | 华南理工大学 | Nanocellulose/lignocellulose composite paper-based saliva glucose detection chip |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3092465A (en) * | 1960-03-25 | 1963-06-04 | Miles Lab | Diagnostic test device for blood sugar |
| US3607093A (en) * | 1968-02-15 | 1971-09-21 | Schering Corp | Devices for testing biological liquids |
| US4144306A (en) * | 1977-01-14 | 1979-03-13 | Eastman Kodak Company | Element for analysis of liquids |
| CN1116307A (en) * | 1986-08-13 | 1996-02-07 | 生命扫描有限公司 | Minimum procedure system for the decermination of analytes |
| CN1156251A (en) * | 1995-08-03 | 1997-08-06 | 生命扫描有限公司 | Test paper for directly reading result |
| CN1761877A (en) * | 2003-03-27 | 2006-04-19 | 泰尔茂株式会社 | Test paper and porous membrane |
| CN101023354A (en) * | 2004-09-20 | 2007-08-22 | 拜尔保健有限公司 | Biological test strip |
-
2011
- 2011-12-27 CN CN201110442697.4A patent/CN102520182B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3092465A (en) * | 1960-03-25 | 1963-06-04 | Miles Lab | Diagnostic test device for blood sugar |
| US3607093A (en) * | 1968-02-15 | 1971-09-21 | Schering Corp | Devices for testing biological liquids |
| US4144306A (en) * | 1977-01-14 | 1979-03-13 | Eastman Kodak Company | Element for analysis of liquids |
| CN1116307A (en) * | 1986-08-13 | 1996-02-07 | 生命扫描有限公司 | Minimum procedure system for the decermination of analytes |
| CN1156251A (en) * | 1995-08-03 | 1997-08-06 | 生命扫描有限公司 | Test paper for directly reading result |
| CN1761877A (en) * | 2003-03-27 | 2006-04-19 | 泰尔茂株式会社 | Test paper and porous membrane |
| CN101023354A (en) * | 2004-09-20 | 2007-08-22 | 拜尔保健有限公司 | Biological test strip |
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| Publication number | Publication date |
|---|---|
| CN102520182A (en) | 2012-06-27 |
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