CN105651765A - Rapid protein test paper, and production method and detection method thereof - Google Patents
Rapid protein test paper, and production method and detection method thereof Download PDFInfo
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- CN105651765A CN105651765A CN201511003461.5A CN201511003461A CN105651765A CN 105651765 A CN105651765 A CN 105651765A CN 201511003461 A CN201511003461 A CN 201511003461A CN 105651765 A CN105651765 A CN 105651765A
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- test paper
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- detection test
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention discloses a rapid protein test paper. The test paper comprises a substrate and a chromogenic test paper fixedly arranged on the substrate, and the chromogenic test paper is produced through fully moistening a filter paper in a dipping liquid and through drying; and the dipping liquid includes, by mass, 0.5-10% of an alkali agent, 0.1-0.6% of copper sulfate, 0.3-1.2% of a complexing agent and 1.0-4.0% of a dispersant. The rapid protein test paper has the advantages of simple operation, no need of preparation of a reagent, fast detection speed, suitableness for online detection, solving of the disadvantages of time consumption and labor consumption in detection of proteins by using traditional large apparatuses, simple production, low cost, low detection cost, accuracy and good repeatability of a detection result, and good application prospect.
Description
Technical field
The invention belongs to the technical field analyzing detection, be specifically related to a kind of protein quick detection test paper and preparation method thereof and detection method.
Background technology
Protein, as the material base of life, is that human body carries out tissue renewal and the primary raw material repaired, is also most important nutrient in human body. The nitrogen element of needed by human body, obtains only by the protein in food, so protein has saccharide and the irreplaceable effect of fat for human body. Therefore, the detection of protein content, also become an important indicator in food inspection.
The method being currently performed protein detection has a variety of, e.g., and Kjeldahl's method, spectrophotography, ultraviolet absorption method etc. Wherein, Kjeldahl's method is the protein detection method of GB, but the method operation is excessively complicated, and whole testing process is time-consuming long, is not suitable for the quick detection of Protein in Food. Spectrophotography, ultraviolet absorption method detection speed, however it is necessary that preparation substantial amounts of detectable, not Portable belt, and requires that testing staff has certain professional standards.
Summary of the invention
In order to solve length consuming time, the defect such as complicated operation, not Portable belt that existing protein detection techniques exists, the invention provides a kind of detect rapidly, protein quick detection test paper that detection sensitivity is high and preparation method thereof and detection method.
The invention provides a kind of protein quick detection test paper, this Test paper includes substrate and the display reagent paper being fixed on substrate, and colour developing reagent paper is that filter paper is through impregnation with dry after liquid is sufficiently humidified so as to preparing; Impregnation liquid comprises the component of following mass percent: alkaline agent 0.5-10%, copper sulfate 0.1-0.6%, chelating agent 0.3-1.2%, dispersant 1.0-4.0%. Substrate is made up of PVC (polrvinyl chloride) material, and substrate is preferably white, it is simple to observe the color change of colour developing reagent paper. Impregnation liquid be a kind of can with the alkaline cupric test solution of protein solution generation biuret chromogenic reaction, in blueness, when containing peptide bond, copper in test solution and peptide ligands, coordination compound is purple. The depth of the color of purple is directly proportional to protein concentration, admittedly the content of protein can be determined. Dispersant makes the color of Test paper evenly, and color developing effect is better.
Preferably, alkaline agent is sodium hydroxide or potassium hydroxide.
Preferably, chelating agent is sodium potassium tartrate tetrahydrate or disodiumedetate.
Preferably, dispersant is polyvinylpyrrolidone.
Preferably, impregnation liquid also comprises the component of following mass percent: surfactant 1.0-3.0%, cosurfactant 0.5-1.5%. Surfactant and cosurfactant make the distribution of color of reagent paper after colour developing uniform, and color effects is good, and testing result is best.
Preferably, surfactant is any one in Tween 80, Triton X-100, dodecylbenzene sodium sulfonate.
Preferably, cosurfactant is any one in dehydrated alcohol, normal propyl alcohol, n-butyl alcohol, hexanol, n-octyl alcohol, ethylene glycol, propylene glycol, glycerol, polyglycerin ester.
The preparation method that present invention also offers a kind of protein quick detection test paper, the method comprises the following steps:
(1) filter paper is impregnated in impregnation liquid 1-10min, dry after taking-up, prepare colour developing reagent paper; Impregnation liquid comprises the component of following mass percent: alkaline agent 0.5-10%, copper sulfate 0.1-0.6%, chelating agent 0.3-1.2%, dispersant 1.0-4.0%;
(2) dried colour developing reagent paper is pasted onto on substrate.
Dry can employing is dried under lyophilizing, air-dry, room temperature or the method such as heat drying.
Preferably, the impregnation liquid of step (1) also comprises the component of following mass percent: surfactant 1.0-3.0%, cosurfactant 0.5-1.5%.
Invention further provides a kind of method utilizing Test paper quickly to detect protein content, Test paper is placed in each gradient sample of standard protein solution and testing sample solution 3-5s, keep flat after taking-up, its reflective value is measured after 3-10min, with the concentration of each gradient sample of standard protein solution and reflective value fit standard curve, the reflective value of testing sample solution is substituted into standard curve calculates protein content.
Reagent paper, when test, only need to be immersed testing protein liquid several seconds by the Test paper of the present invention, horizontal after taking-up, after colour developing reagent paper colour stable, can be undertaken detecting or visual colorimetry analysis by photoelectric color measurement instrument, thus the protein content measured in liquid. The detection method of the present invention can complete the protein content determination of testing sample within a few minutes, it is achieved the quick detection of protein.
Beneficial effects of the present invention: the Test paper of the present invention is simple to operate, without reagent preparation, detection speed is fast, it is suitable for on-line checking, overcomes the deficiency wasted time and energy when using traditional large-scale instrument to protein detection, and reagent paper makes simple, with low cost, testing cost is low, testing result is accurate, reproducible, has a good application prospect.
Accompanying drawing explanation
Fig. 1 is the structural representation of present protein quick detection test paper;
1, substrate; 2, colour developing reagent paper.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is carried out explanation explained in detail.
Embodiment 1
A kind of protein quick detection test paper, this Test paper includes substrate and the display reagent paper being fixed on substrate, and colour developing reagent paper is that filter paper is through impregnation with dry after liquid is sufficiently humidified so as to preparing; Impregnation liquid comprises the component of following mass percent: potassium hydroxide 0.5%, copper sulfate 0.1%, sodium potassium tartrate tetrahydrate 0.3%, polyvinylpyrrolidone 1.0%.
A kind of preparation method of protein quick detection test paper, the method comprises the following steps:
(1) weigh 0.5g potassium hydroxide, 0.1g copper sulfate, 0.3g sodium potassium tartrate tetrahydrate, dissolve with 50mL distilled water;Be subsequently adding the polyvinylpyrrolidone of 1.0g, stir, after with distilled water diluting to 100mL, prepare impregnation liquid.
(2) being placed in by filter paper in impregnation liquid and impregnate 1min, take out, in 55 DEG C of drying baker, dry 20min, is pasted onto on substrate by dried colour developing reagent paper, prepares described Test paper.
The method that described Test paper quickly detects protein content is utilized to be: Test paper is placed in each gradient sample of standard protein solution and testing sample solution 3s, keep flat after taking-up, its reflective value is measured after 10min, with the concentration of each gradient sample of standard protein solution and reflective value fit standard curve, the reflective value of testing sample solution is substituted into standard curve calculates protein content.
Embodiment 2
A kind of protein quick detection test paper, this Test paper includes substrate and the display reagent paper being fixed on substrate, and colour developing reagent paper is that filter paper is through impregnation with dry after liquid is sufficiently humidified so as to preparing; Impregnation liquid comprises the component of following mass percent: sodium hydroxide 4.0%, copper sulfate 0.4%, disodiumedetate 0.6%, glycine 1.8%, sodium chloride 0.14%, polyvinylpyrrolidone 3.0%, Tween 80 1.0%, dehydrated alcohol 0.5%.
A kind of preparation method of protein quick detection test paper, the method comprises the following steps:
(1) 4.0g sodium hydroxide, 0.4g copper sulfate, 0.6g disodiumedetate, 1.8g glycine, 0.14g sodium chloride are weighed, dissolve with 50mL distilled water, it is subsequently adding 3.0g polyvinylpyrrolidone, 1.0g Tween 80,0.5g dehydrated alcohol, mix homogeneously, afterwards with distilled water diluting to 100mL, prepare impregnation liquid.
(2) filter paper is placed in impregnation liquid and impregnates 5min, take out, dry 15min in 60 DEG C of drying baker; Dried colour developing reagent paper is pasted onto on substrate, prepares described Test paper.
The method that described Test paper quickly detects protein content is utilized to be: Test paper is placed in each gradient sample of standard protein solution and testing sample solution 4s, keep flat after taking-up, its reflective value is measured after 3min, with the concentration of each gradient sample of standard protein solution and reflective value fit standard curve, the reflective value of testing sample solution is substituted into standard curve calculates protein content.
Embodiment 3
A kind of protein quick detection test paper, this Test paper includes substrate and the display reagent paper being fixed on substrate, and colour developing reagent paper is that filter paper is through impregnation with dry after liquid is sufficiently humidified so as to preparing; Impregnation liquid comprises the component of following mass percent: sodium hydroxide 10%, copper sulfate 0.6%, sodium potassium tartrate tetrahydrate 1.2%, polyvinylpyrrolidone 4.0%, dodecylbenzene sodium sulfonate 3.0%, polyglycerin ester 1.5%.
A kind of preparation method of protein quick detection test paper, the method comprises the following steps:
(1) 10g sodium hydroxide, 0.6g copper sulfate, 1.2g sodium potassium tartrate tetrahydrate are weighed, dissolve with 50mL distilled water, be subsequently adding the polyvinylpyrrolidone of 4.0g, 3.0g dodecylbenzene sodium sulfonate, 1.5g polyglycerin ester, mix homogeneously, afterwards with distilled water diluting to 100mL, prepare impregnation liquid.
(2) filter paper is placed in impregnation liquid and impregnates 10min, take out, dry 20min in 55 DEG C of drying baker; Dried colour developing reagent paper is pasted onto on substrate, prepares described Test paper.
The method that described Test paper quickly detects protein content is utilized to be: Test paper is placed in each gradient sample of standard protein solution and testing sample solution 5s, keep flat after taking-up, its reflective value is measured after 5min, with the concentration of each gradient sample of standard protein solution and reflective value fit standard curve, the reflective value of testing sample solution is substituted into standard curve calculates protein content.
Comparative example 1
The detection method of existing protein detection reagent paper:
(1) preparation of biuret reagent: weigh 1.90g copper sulfate, 2.85gEDTA-Na2��2H2O, 8.75g glycine, 7.00g sodium chloride, use 350mL distilled water dissolve, and under agitation add 28.00g sodium hydroxide, with distilled water diluting to 500mL, store for future use.
(2) the solution 100mL of 1.5 times of biuret standard reagent concentration is prepared, add TritonX 1000.35g, polyvinylpyrrolidone 0.25g, after dissolving, reagent paper is placed in solution and fully soaks 10min, taking out, in 60 DEG C of drying baker, dry 15min, is pasted onto on PV hectograph by dried reagent paper, it is cut into the little bar of 2cm �� 0.5cm, puts in the bottle added with desiccant and preserve.
(3) each 20 �� L of protein standard solution taking variable concentrations drip on reagent paper, put into photoelectric detector, detect, with the concentration of each gradient sample of standard protein solution and reflective value fit standard curve under wavelength 560nm after reaction 10min. Take sample solution, respectively take 20 �� L and drip on reagent paper, put into photoelectric detector after reaction 10min, detect under wavelength 560nm, record result, standard curve is found protein concentration, calculates protein content (g/100g).
The detection of protein is limited to 0.5mg/mL by the Test paper method of the present invention, and the detection of protein is limited to 0.85mg/mL by existing Test paper method, and the repeatability of the Test paper method of the present invention is better than existing Test paper method.
Presently preferred embodiments of the present invention, not in order to limit the present invention, all any amendment, equivalent replacement and simple modifications etc. made in flesh and blood of the present invention, should be included within protection scope of the present invention.
Claims (10)
1. a protein quick detection test paper, it is characterised in that this Test paper includes substrate and the display reagent paper being fixed on substrate, colour developing reagent paper is that filter paper is through impregnation with dry after liquid is sufficiently humidified so as to preparing; Impregnation liquid comprises the component of following mass percent: alkaline agent 0.5-10%, copper sulfate 0.1-0.6%, chelating agent 0.3-1.2%, dispersant 1.0-4.0%.
2. protein quick detection test paper according to claim 1, it is characterised in that alkaline agent is sodium hydroxide or potassium hydroxide.
3. protein quick detection test paper according to claim 1, it is characterised in that chelating agent is sodium potassium tartrate tetrahydrate or disodiumedetate.
4. protein quick detection test paper according to claim 1, it is characterised in that dispersant is polyvinylpyrrolidone.
5. protein quick detection test paper according to claim 1, it is characterised in that impregnation liquid also comprises the component of following mass percent: surfactant 1.0-3.0%, cosurfactant 0.5-1.5%.
6. protein quick detection test paper according to claim 5, it is characterised in that surfactant is any one in Tween 80, Triton X-100, dodecylbenzene sodium sulfonate.
7. protein quick detection test paper according to claim 5, it is characterised in that cosurfactant is any one in dehydrated alcohol, normal propyl alcohol, n-butyl alcohol, hexanol, n-octyl alcohol, ethylene glycol, propylene glycol, glycerol, polyglycerin ester.
8. the method for the protein quick detection test paper that a kind is prepared described in claim 1, it is characterised in that the method comprises the following steps:
(1) filter paper is impregnated in impregnation liquid 1-10min, dry after taking-up, prepare colour developing reagent paper;Impregnation liquid comprises the component of following mass percent: alkaline agent 0.5-10%, copper sulfate 0.1-0.6%, chelating agent 0.3-1.2%, dispersant 1.0-4.0%;
(2) dried colour developing reagent paper is pasted onto on substrate.
9. the preparation method of protein quick detection test paper according to claim 8, it is characterised in that the impregnation liquid of step (1) also comprises the component of following mass percent: surfactant 1.0-3.0%, cosurfactant 0.5-1.5%.
10. the method for the Test paper detection protein content that a kind utilizes described in claim 1, it is characterized in that, Test paper is placed in each gradient sample of standard protein solution and testing sample solution 3-5s, keep flat after taking-up, its reflective value is measured after 3-10min, with the concentration of each gradient sample of standard protein solution and reflective value fit standard curve, the reflective value of testing sample solution is substituted into standard curve calculates protein content.
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| CN201511003461.5A CN105651765A (en) | 2015-12-28 | 2015-12-28 | Rapid protein test paper, and production method and detection method thereof |
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| CN201511003461.5A CN105651765A (en) | 2015-12-28 | 2015-12-28 | Rapid protein test paper, and production method and detection method thereof |
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Cited By (6)
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| CN106525829A (en) * | 2016-10-31 | 2017-03-22 | 百奥森（江苏）食品安全科技有限公司 | Test paper for protein rapid detection and preparation method thereof |
| CN108548814A (en) * | 2018-04-19 | 2018-09-18 | 长春万成生物电子工程有限公司 | The detection method of bromine content in Test paper of bromine content and preparation method thereof and water |
| CN109991220A (en) * | 2019-03-29 | 2019-07-09 | 迪瑞医疗科技股份有限公司 | A kind of preparation method of phlorose aldehydic acid enzyme Test paper |
| CN110108700A (en) * | 2019-04-18 | 2019-08-09 | 迪瑞医疗科技股份有限公司 | A kind of polyamines detection reagent, polyamines detection dry chemical scrip and preparation method thereof |
| CN110824174A (en) * | 2019-11-28 | 2020-02-21 | 南京迪安医学检验所有限公司 | Reagent for detecting total serum protein and preparation method thereof |
| KR20210043891A (en) * | 2019-10-14 | 2021-04-22 | 김원진 | Test paper for measuring protein concentration and measuring device for protein concentration using the same |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106525829A (en) * | 2016-10-31 | 2017-03-22 | 百奥森（江苏）食品安全科技有限公司 | Test paper for protein rapid detection and preparation method thereof |
| CN108548814A (en) * | 2018-04-19 | 2018-09-18 | 长春万成生物电子工程有限公司 | The detection method of bromine content in Test paper of bromine content and preparation method thereof and water |
| CN109991220A (en) * | 2019-03-29 | 2019-07-09 | 迪瑞医疗科技股份有限公司 | A kind of preparation method of phlorose aldehydic acid enzyme Test paper |
| CN110108700A (en) * | 2019-04-18 | 2019-08-09 | 迪瑞医疗科技股份有限公司 | A kind of polyamines detection reagent, polyamines detection dry chemical scrip and preparation method thereof |
| KR20210043891A (en) * | 2019-10-14 | 2021-04-22 | 김원진 | Test paper for measuring protein concentration and measuring device for protein concentration using the same |
| KR102248674B1 (en) * | 2019-10-14 | 2021-05-06 | 김원진 | Test paper for measuring protein concentration and measuring device for protein concentration using the same |
| CN110824174A (en) * | 2019-11-28 | 2020-02-21 | 南京迪安医学检验所有限公司 | Reagent for detecting total serum protein and preparation method thereof |
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