CN105606796A - Nitrocellulose membrane pretreatment method applied to gold immunochromatography assay - Google Patents
Nitrocellulose membrane pretreatment method applied to gold immunochromatography assay Download PDFInfo
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- CN105606796A CN105606796A CN201610066693.3A CN201610066693A CN105606796A CN 105606796 A CN105606796 A CN 105606796A CN 201610066693 A CN201610066693 A CN 201610066693A CN 105606796 A CN105606796 A CN 105606796A
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- 239000000020 Nitrocellulose Substances 0.000 title claims abstract description 40
- 229920001220 nitrocellulos Polymers 0.000 title claims abstract description 40
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 239000012528 membrane Substances 0.000 title claims abstract description 17
- 238000002203 pretreatment Methods 0.000 title claims abstract description 11
- 239000010931 gold Substances 0.000 title abstract description 6
- 229910052737 gold Inorganic materials 0.000 title abstract description 6
- 238000003556 assay Methods 0.000 title abstract 2
- 238000003317 immunochromatography Methods 0.000 title abstract 2
- 238000001035 drying Methods 0.000 claims abstract description 6
- 210000004379 membrane Anatomy 0.000 claims description 9
- 229920002160 Celluloid Polymers 0.000 claims description 8
- 238000004026 adhesive bonding Methods 0.000 claims description 3
- 210000004400 mucous membrane Anatomy 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 10
- 238000002360 preparation method Methods 0.000 description 17
- 239000000243 solution Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 9
- 238000001514 detection method Methods 0.000 description 7
- 230000002209 hydrophobic effect Effects 0.000 description 6
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 6
- 239000012224 working solution Substances 0.000 description 6
- 108010048233 Procalcitonin Proteins 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- CWCXERYKLSEGEZ-KDKHKZEGSA-N procalcitonin Chemical compound C([C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)NCC(O)=O)[C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCSC)NC(=O)[C@H]1NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@@H](N)CSSC1)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 CWCXERYKLSEGEZ-KDKHKZEGSA-N 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 239000005030 aluminium foil Substances 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 239000000084 colloidal system Substances 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 239000001509 sodium citrate Substances 0.000 description 3
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 3
- 229940038773 trisodium citrate Drugs 0.000 description 3
- 241001494479 Pecora Species 0.000 description 2
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920006267 polyester film Polymers 0.000 description 2
- 229940072033 potash Drugs 0.000 description 2
- 235000015320 potassium carbonate Nutrition 0.000 description 2
- 229910000027 potassium carbonate Inorganic materials 0.000 description 2
- 235000011181 potassium carbonates Nutrition 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000011241 protective layer Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000012502 diagnostic product Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000003325 tomography Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a nitrocellulose membrane pretreatment method applied to gold immunochromatography assay. The method comprises the following steps at the time: 1, sticking a nitrocellulose membrane with a backing and a PVC plate; 2, putting the adhered nitrocellulose membrane into an air dry oven for drying under the temperature of 20 to 37 DEG C for 4 to 10 hours; 3, placing the dried nitrocellulose membrane under the environment with the temperature of 18 to 28 DEG C and the humidity of 60 to 70 percent, and performing shading balance for 1 to 2 hours; 4, specking the membrane. According to the nitrocellulose membrane pretreatment method, on the one hand, the quality of a membrane specking line is improved, and on the other hand, the repetitiveness and the lot tolerance of products are enhanced.
Description
Technical field
The invention belongs to colloidal gold immunochromatographimethod technology, be specifically related to a kind of celluloid that is applied to colloidal gold immunochromatographimethodMembrane pretreatment method.
Background technology
Colloidal gold immunochromatographimethod technology is with its high specificity, the feature such as highly sensitive, simple to operation, medical science, farming and animal husbandry,The field such as environment and food inspection is widely used. The course of reaction of collaurum skill tomography be one by nanogold particle, anti-Former and antibody are in conjunction with the course of reaction of chromatography on test paper, and in this process, the quality of each link directly affect to detect and tiesThe accuracy of fruit.
Celluloid membrane material is because have that low cost, capillary flow are stable, high protein binding ability, process relatively and holdEasily (there is the film of polyester backing) and have the feature of different rate of water absorption and surface active composition, thereby by most of immunity layersAnalysing diagnostic products system selects.
Although nitrocellulose filter has these positive characteristics, nitrocellulose filter also has many defects. These defectsComprise: function is subject to environmental change (for example humidity) impact performance and poor repeatability between criticizing in batch.
In existing colloidal gold immunochromatographimethod method, the film of harsh output generally contains the moisture of 5-10%. Old about filmChange mechanism, have theoretical a support, but dispute is larger. Theory is thought: the aging of film is because the moisture evaporation on film makesIt is hydrophobic that film becomes, electrically charged and become fragile. Storage films General Requirements is lucifuge, sealing. Overdrying or excessively wet all unfavorable. In this preservationUnder condition, generally can place 2 years. Humidity is too low, easy static electricity gathered lotus on film, and easily there is loose point in some film, causes testingThere will be hydrophobic spot. Humidity is too high, and on film, capillarity is strengthened, and some film easily causes the diffusion that broadens even of CT line. In order to ensureThe homogeneity of film humidity when point sample, improve batch in repeatability and batch between repeatability, this patent has been invented a kind of new cellulose nitrateElement film pretreatment side method.
Summary of the invention
1, the object of the invention is the above-mentioned deficiency for prior art, a kind of colloidal gold immunochromatographimethod that is applied to is providedCelluloid membrane pretreatment method.
2, a celluloid membrane pretreatment method for colloidal gold immunochromatographimethod, in turn includes the following steps: (1) is stickyFilm: nitrocellulose filter and backing, PVC plate stick together (2) dry: the nitrocellulose filter gluing is put into air dry oven20 ° of dry 4-10 hour (3) balances of C-37 ° of C: it is 18 DEG C-28 DEG C that dried nitrocellulose filter is placed on temperature, humidity 60%-Under 70% state, 1-2 hour (4) some film of lucifuge balance.
3, preferably 20～37 DEG C of described baking temperatures, preferably 4～10 hours drying time.
4, further preferably 20 DEG C of described baking temperatures, further preferably 4 hours drying time.
5,18 DEG C-28 DEG C of described equilibrium temperatures, under humidity 60%-70% state, lucifuge balance 1-2 hour.
6, more preferably 20 DEG C of described equilibrium temperatures, under humidity 65% state, lucifuge balance 1 hour.
7, described the desk-top or continuity point film machine point film that film is standard.
Beneficial effect of the present invention: nitrocellulose filter, by pretreatment, is criticized interior nitrocellulose filter in stroke membrane process,Draw film liquid and occur that hydrophobic spot rate and diffusivity obviously decline; The colloid gold test paper that nitrocellulose filter is made after by pretreatmentCard, add sample survey, in its batch repeatability and batch between repeatability further optimized, can obviously improve colloid gold test paperThe accuracy and the detection efficiency that detect.
Detailed description of the invention
Embodiment 1
1, mucous membrane: the protective layer of the diaphragm position on PVC base plate is taken off, exposed binder, at the bottom of nitrocellulose filter and PVCThe alignment of plate relevant position is pasted.
2, dry: the PVC base plate that glues nitrocellulose filter is put into air dry oven inner drying, select 20 DEG C of temperature,Dry 4 hours, dried nitrocellulose filter is put into the aluminium foil bag sealing preservation that drier is housed, the term of validity is 3Month.
3, balance: take out the nitrocellulose filter that was dried 20 DEG C of temperature, humidity 65%, places 1 little in the environment of lucifugeShi Pingheng.
4, the preparation of nature controlling line working solution: the sheep anti mouse that is solvent preparation 0.9mg/ml with the PBS solution of 3% trehalose is manyClonal antibody solution.
5, the preparation of detection line working solution: the Procalcitonin that is solvent preparation 0.7mg/ml with the PBS solution of 3% trehaloseMonoclonal antibody solution.
6, some film: utilize desk-top some film metal spraying machine that the nature controlling line preparing in advance and detection line working solution are crossed in balanceOn nitrocellulose filter, put film, some film speed is 1ul/cm, and nature controlling line is parallel with detection line, and the two lines 6mm of being separated by is careful even,Put into 37 DEG C of forced air dryings of air dry oven 20 hours.
Embodiment 2
1, in order to further illustrate the pretreated superiority of the nitrocellulose filter being used in colloidal gold immunity chromatography, IAdopted same colloidal gold immunity chromatography, nitrocellulose filter pretreatment is made into nitrocellulose filter not carry outPreprocess method, applies in the practical application of Procalcitonin detection reagent card,
The concrete preparation method of colloidal gold strip is as follows:
1) preparation of coated film:
A) mucous membrane: the protective layer of the diaphragm position on PVC base plate is taken off, exposed binder, at the bottom of nitrocellulose filter and PVCThe alignment of plate relevant position is pasted, and the nitrocellulose filter gluing is divided into two parts, and portion is directly put into aluminium foil bag sealing and preserved,Another part carries out pretreatment according to embodiment 1 method to nitrocellulose filter;
B) preparation of nature controlling line working solution: the sheep anti mouse polyclone that is solvent preparation 0.9mg/ml with the PBS solution of 3% trehaloseAntibody-solutions;
C) preparation of detection line working solution: the Procalcitonin Dan Ke that is solvent preparation 0.7mg/ml with the PBS solution of 3% trehaloseGrand antibody-solutions;
D) some film: utilize desk-top some film metal spraying machine by the nature controlling line preparing in advance and detection line working solution respectively pretreatedNitrocellulose filter and not pretreated nitrocellulose filter on put film, some film speed is 1ul/cm, nature controlling line and detectionLine parallel, the two lines 6mm of being separated by, careful evenly, put into 37 DEG C of forced air dryings of air dry oven 20 hours;
E) the dried nitrocellulose filter of appeal is placed in the aluminium foil bag that drier is housed, sealing is preserved.
2) preparation of the polyester film of coated with gold labeling antibody:
A) preparation of aqueous solution of chloraurate: the tri-distilled water of 10g gold chloride 1000ml is dissolved, be made into the aqueous solution of l%, be placed in 4DEG C for subsequent use, the term of validity 3 months;
B) preparation of trisodium citrate: dissolve trisodium citrate with tri-distilled water, be made into the aqueous solution of l%, be placed in 4 DEG C for subsequent use, have7 days effect phases;
C) preparation of l% wet chemical: 1g potash is dissolved with 100ml tri-distilled water, be placed in 4 DEG C for subsequent use, the term of validityIt is 7 days;
D) golden labeling antibody is preserved the preparation of liquid: by the sucrose of 15g, and the Sodium azide of 20ul, molten at the 1%BSA of the pH=7.4 of 100mlSeparate, be placed in 4 DEG C for subsequent use, the term of validity is 7 days;
E) preparation of the particle of collaurum: 1% gold chloride is diluted to 0.01% with tri-distilled water, is placed in 95 DEG C of reactions 10
Minute, add 1ml trisodium citrate, continue reaction 15 minutes, until colloidal gold solution color by indigo plant after purple stain is red, coolingAfter add 2ml1% solution of potassium carbonate for subsequent use. Outward appearance corridor is pure, bright, without precipitation and floating thing;
F) preparation of golden labeling antibody: by 1% solution of potassium carbonate adjusting collaurum pH value to 7.5, add applicable Procalcitonin listClonal antibody solution fully mixes, regulate pH to 5.0, react after 10～30 minutes, cooling after, use while stirring 1% potash waterSolution slowly drips, regulator solution PH to 7.5, and stable stirring 10 minutes, is then placed in 25 DEG C of water-baths and adds after 30 minutes againEnter 5%BSA, seal after 20 minutes, centrifuging and taking precipitation, recovers its final concentration with BSA and is 1%, 4 DEG C and saves backup;
G) good mark antibody colloidal gold is layered on polyester film uniformly, discharge rate is 1.0ul/cm, dry, and envelope is for subsequent use.
3) processing of sample pad
Sample pad is soaked to a few hours with 100mMPBs buffer solution, after taking-up is dry.
4) assembling of colloidal gold strip:
End liner 1, sample pad 2 and blotting paper 5 are the general parts in this area. By above-mentioned sample pad 2, be coated with the poly-of golden labeling antibodyEster film 3, coated film 4 and blotting paper 5 in turn mutually overlap joint paste and obtain test paper plate, finally test paper plate is cut into different in widthTest strips.
The test strips that nitrocellulose filter through pretreated and untreated nitrocellulose filter are prepared is carried outThe contrast of repeatability. By contrast, we can see hydrophobic spot rate and the diffusivity ratio of the nitrocellulose filter of treated mistakeUntreated nitrocellulose filter is low; Meanwhile, the test card of making through pretreated nitrocellulose filter batch in repetitionProperty and I criticize between repeatability be starkly lower than the test card that untreated nitrocellulose filter is made, we therefrom draw nitric acid fibreTie up the colloid gold test paper that plain film is made after treatment, the more untreated nitrocellulose filter of its quality and the degree of accuracy has very greatly to be carriedHigh. Correction data is as follows:
Table one, nitrocellulose filter are without pretreatment
Table two, nitrocellulose filter have pretreatment
Remarks: nitrocellulose filter part is too dry will produce hydrophobic spot, and too moistening stroke of film line will spread and come, with30cm length is that the nitrocellulose filter of calculates, and the total length that occurs breakpoint obtains percentile divided by 30 and is hydrophobic spotRate, drawing film width is 1mm, the total length that exceedes 1.5mm region obtains hundredths element divided by 30 and is diffusivity.
In above embodiment, various processes and the method do not described in detail are conventional methods as known in the art.
The above, be only preferred embodiment of the present invention and oneself, is not the restriction of the present invention being made to other form, appointsWhat those skilled in the art may utilize the technology contents of above-mentioned announcement changed or be modified as equivalent variations etc.Effect embodiment. But every technical solution of the present invention content that do not depart from, according to technical spirit of the present invention to above embodiment instituteAny simple modification, equivalent variations and the remodeling done, still belong to the protection domain of technical solution of the present invention.
Claims (4)
1. a celluloid membrane pretreatment method that is applied to colloidal gold immunochromatographimethod, is characterized in that: comprise successively asLower step: mucous membrane: nitrocellulose filter and backing, PVC plate stick together; (2) dry: the nitrocellulose filter gluing is put intoDry in air dry oven; (3) balance; (4) some film.
2. according to a kind of celluloid membrane pretreatment method that is applied to colloidal gold immunochromatographimethod described in claim l, itsBe characterised in that: described drying condition is in blast drier, temperature is 20 DEG C-37 DEG C, and be 4-10 minute drying time.
3. a kind of celluloid membrane pretreatment method that is applied to colloidal gold immunochromatographimethod according to claim 1, itsBe characterised in that: described equilibrium condition is: lucifuge or ruddiness condition, temperature is 18 DEG C-28 DEG C, humidity is 60%-70%, balanceTime is 1-2 hour.
4. according to a kind of celluloid membrane pretreatment method that is applied to colloidal gold immunochromatographimethod described in claim l, itsBe characterised in that: described some film can be used continuity point film machine, desk-top some film machine equipment point film.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226487A (en) * | 2016-12-22 | 2018-06-29 | 中粮集团有限公司 | Zearalenone detection card and the zearalenone detection method blocked using the detection |
| CN108241059A (en) * | 2016-12-23 | 2018-07-03 | 中粮集团有限公司 | Fumonisin detects colloidal gold quick measuring card and kit and the method being detected to fumonisin |
| CN108241061A (en) * | 2016-12-23 | 2018-07-03 | 中粮集团有限公司 | Vomitoxin detects colloidal gold quick measuring card and kit and the method being detected to vomitoxin |
| CN109541234A (en) * | 2018-11-23 | 2019-03-29 | 东莞东阳光科研发有限公司 | A kind of preparation method of accurate quantification detection immuno-chromatographic test paper strip |
| CN112730823A (en) * | 2020-12-31 | 2021-04-30 | 广州安诺科技股份有限公司 | Method for treating nitrocellulose membrane and colloidal gold detection card |
| CN113085304A (en) * | 2021-05-10 | 2021-07-09 | 苏州天硕健康科技有限公司 | NC membrane with strong protein binding capacity for colloidal gold immunoassay |
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