CN206573587U - immune lateral chromatography detecting system - Google Patents
immune lateral chromatography detecting system Download PDFInfo
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- CN206573587U CN206573587U CN201621147170.3U CN201621147170U CN206573587U CN 206573587 U CN206573587 U CN 206573587U CN 201621147170 U CN201621147170 U CN 201621147170U CN 206573587 U CN206573587 U CN 206573587U
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- label
- lateral chromatography
- nitrocellulose filter
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Abstract
The utility model discloses a kind of immune lateral chromatography detecting system, wherein, detecting system includes：Bottom plate, it includes nearly sample end and remote sample end；On described bottom plate sample pad, nitrocellulose filter and adsorptive pads are provided with by nearly sample end with being sequentially connected to remote sample end；Label band is provided with described nitrocellulose filter, detection band and quality control band are provided between described label band and adsorptive pads.Immune lateral chromatography detecting system of the present utility model has that cost is low, detect accurate advantage.
Description
Technical field
The utility model is related to medical domain, and in particular to a kind of immune lateral chromatography detecting system.
Background technology
Immune lateral chromatography diagnostic techniques is adapted in various real-time test as a kind of stabilization and practical technology
Or onsite application (POCT).Being divided into mainly by mark part has colloid gold immune lateral chromatography method, common fluorescent immune lateral
Chromatography, time-resolved fluoroimmunoassay lateral chromatography method, on turn electrochemiluminescent immunoassay lateral chromatography method, quantum dot fluorescence lateral layer be immunized
Analysis method etc., mainly has nitrocellulose filter, composite (fusion5), micro-fluidic etc. by coating part.
With the development of immunochromatography technique (immunochromatography) and colloidal gold technique, the especially nineties
Afterwards, diagnose the illness colloid gold immune lateral chromatography method (Gold immunochromatography assay, GICA) inspection in vitro
It is widely applied in survey.But quantitatively detected now, more and more higher is required to project precision and repeatability, passed through
The development in many years, nearest all kinds of fluorescence immunoassay lateral chromatography methods emerge in an endless stream.Main flow advanced technology is turned into by many public affairs
Department praises highly.
However, existing immune lateral chromatography method it is general all by label soak or be sprayed in special labeling pad or
On, the shortcomings of doing so is as follows：Component is numerous, influences the release for climbing water and label of product；Because component is numerous, produce
To bother, take artificial, the cost of product, the significant wastage of social resources are improved indirectly.Also label is put into fluorescence by some
In pipette tips, the method operation requires high, causes not easy.Label consumption is big, causes production cost high.
Utility model content
The purpose of this utility model is to provide a kind of immune lateral chromatography detecting system, detecting system of the present utility model
The component of detecting system is reduced by the way that label is arranged on nitrocellulose filter, so that it is aqueous to improve climbing for detecting system
Can, reduction operation difficulty and production cost.
The purpose of this utility model is realized in the following way：
A kind of immune lateral chromatography detecting system, including：
Bottom plate, it includes nearly sample end and remote sample end；Connected successively to remote sample end by nearly sample end on described bottom plate
Ground connection is provided with sample pad, nitrocellulose filter and adsorptive pads；Label band is provided with described nitrocellulose filter, it is described
Label band and adsorptive pads between be provided with detection band and quality control band.
Further, label is contained on described label band, described label is latex fluorescence, time resolution is glimmering
Light, luminous upper transfer, quantum dot fluorescence, fluorescent dye or collaurum.
Further, containing detection antibody in described detection band, described detection antibody is anti-for c reactive protein detection
Body.
Further, control antibodies are contained on described quality control band, described control antibodies are goat-anti chicken IgY.
Further, the length of described detecting system is 6-8cm, and width is 3-6mm.
On the other hand, the utility model additionally provides a kind of preparation method of immune lateral chromatography system, including following step
Suddenly：
(1) antibody or antigen coat are detected to nitrocellulose filter as detection band using first, by the first contrast agents
It is coated with nitrocellulose filter as quality control band；
(2) described nitrocellulose filter is subjected to Seal treatment with film process liquid；
(3) labelled reagent is prepared：Secondary antibody or antigen, the second contrast agents are marked and obtain described mark examination
Agent；
(4) label band is prepared：Described labelled reagent is coated on described nitrocellulose filter and forms label
Band；
(5) sample pad and adsorptive pads are prepared；
(6) assemble：Described sample pad, nitrocellulose filter and adsorptive pads are fixed on bottom plate and cut out with being sequentially connected
Described detecting system is produced into required size, described distance of the label band away from sample pad is less than described detection band and matter
Control distance of the band away from sample pad.
Further, described Seal treatment method specifically includes following steps：The described nitric acid of film process liquid A immersions is fine
The plain film 50-70min of dimension, then again with dry after film process liquid B immersions 10-30min；
Wherein, the molar concentration of carbonic acid buffer is 10-100mMol/l in described film process liquid A, bovine serum albumin
Weight/mass percentage composition is 0.01-2.0%, and the weight/mass percentage composition of surfactant is 0.1-2.0%；
The molar concentration of phosphate buffer is 10-100mMol/l in described film process liquid B, and the quality percentage of sucrose contains
Measure as 0.1-1.0%, the weight/mass percentage composition of surfactant is 1-20%.
Here the molar concentration of carbonic acid buffer refers to the molar concentration of sodium acid carbonate and sodium carbonate；Phosphate buffer density refers to
The molar concentration of sodium dihydrogen phosphate, disodium hydrogen phosphate and sodium chloride.
Further, described Seal treatment specifically includes following steps：It is that 1-10% ox bloods are pure with mass percent
On albumen spray film process nitrocellulose filter, dry.
Further, mark the label used for latex fluorescence, time-resolved fluorescence in described step (3), above turn
Move luminous, quantum dot fluorescence, fluorescent dye or collaurum.
Compared with prior art, the utility model scheme at least has the following advantages that：
Label is marked directly on nitrocellulose filter by the scheme of the present utility model application, which reduces detecting system
Composition, be conducive to the water of climbing of sample, improve the efficiency and accuracy of detection.
The reduction of component can also reduce production process, production efficiency be improved, so as to reduce the production cost of detecting system.
Label is conducive to sample to be also beneficial to the release of label while climbing water, so on nitrocellulose filter
Make Detection results more obvious, accurate.
The utility model method has carried out Seal treatment to nitrocellulose filter, changes the hydrophobic of nitrocellulose filter
Property, realize and label is coated with onto nitrocellulose filter, and existing is all directly to use nitrocellulose filter, that will mark
Note thing, which is coated with onto nitrocellulose filter, can be such that label combines on nitrocellulose filter, it is impossible to discharge.
Brief description of the drawings
Fig. 1 is that existing immune lateral chromatography system architecture signal in lateral chromatography detecting system is immunized in the utility model
Figure；
Fig. 2 is that a kind of immune lateral chromatography system structure diagram in lateral chromatography detecting system is immunized in the utility model.
Embodiment
For convenience of skilled artisan understands that technical solutions of the utility model, below in conjunction with the accompanying drawings and preferred embodiment pair
The technical solution of the utility model is further elaborated, it will be appreciated that preferred embodiment is the understanding of this programme for convenience, and
Limited not as of the present utility model.
Existing immune lateral chromatography system is all in the presence of (labeling pad or fluorescence pipette tips by label with single component
Etc. form), Fig. 1 is existing immune lateral chromatography system structure diagram (by taking labeling pad as an example), as shown in figure 1, existing
Immune lateral chromatography system include bottom plate 1, sample pad 2 is disposed with by nearly sample end to remote sample end on bottom plate 1, marked
Detection band 5 and quality control band 6 are provided with thing pad 7, nitrocellulose filter 3 and adsorptive pads 4, nitrocellulose filter 3；The utility model
Label is coated with onto nitrocellulose filter by scheme, reduces component；If existing nitrocellulose filter directly will mark
Substance markers mark poor effect to above, influence testing result, it is that nitrocellulose filter has hydrophobicity to analyze its reason, therefore
This programme has carried out Seal treatment to nitrocellulose filter, changes its hydrophobicity, so as to which label coating is arrived into nitric acid
On cellulose membrane, production cost is reduced, manpower and materials have been saved.The utility model scheme is described in detail below.
Fig. 2 is that lateral chromatography system structure diagram is immunized in the utility model, as shown in Fig. 2 a kind of immune lateral chromatography
Detecting system, including：
Bottom plate 1, it includes nearly sample end and remote sample end；Connected successively to remote sample end by nearly sample end on described bottom plate
Ground connection is provided with sample pad 2, nitrocellulose filter 3 and adsorptive pads 4；Label band 8 is provided with described nitrocellulose filter,
Detection band 5 and quality control band 6 are provided between described label band 8 and adsorptive pads 4；The hydrophobicity of nitrocellulose filter 3 is it
The basis combined with the protein such as antibody, while can also play inhibition to the release of label band 8, the utility model is to institute
The surface of nitrocellulose filter 3 stated is handled, and changes its hydrophobicity, reaches and label band 8 is placed on to described nitric acid fibre
The purpose of the plain film of dimension.
Above scheme can realize the mesh that label is coated with onto nitrocellulose filter and reaches reduction testing cost
, herein below on the basis of provide prioritization scheme：
Preferably, containing label on described label band 8, described label is latex fluorescence, time resolution
Fluorescence, luminous upper transfer, quantum dot fluorescence, fluorescent dye or collaurum.
Preferably, containing detection antibody in described detection band 5, described detection antibody is anti-for c reactive protein detection
Body.
Preferably, containing control antibodies on described quality control band 6, described control antibodies are goat-anti chicken IgY.
Preferably, the length of described detecting system is 6-8cm, width is 3-6mm.
Embodiment 1
A kind of preparation method of immune lateral chromatography system, comprises the following steps：
(1) CRP detection antibody 1 is diluted to 2.0mg/ml, another control antibodies 1 (goat-anti chicken IgY) with coating buffer solution
2.0mg/ml is diluted to, above-mentioned two liquid coating is arrived by Sai Duolisi companies (Sartorius) using special coating equipment
Nitrocellulose filter (NC) on form detection band and quality control band respectively, 37 DEG C of drying boxes are dried 4 hours.Being coated with buffer solution is
0.01Mol/l phosphate buffer (PBs) plus mass fraction is used as protective agent for 3% sucrose.
(2) nitrocellulose filter is subjected to Seal treatment：With film process liquid A immersion treatments 1 hour, then with film process liquid B
Immersion treatment 20 minutes, is dried.
Wherein, described film process liquid A includes following component：Wherein CB is molar concentration, and remaining is mass percent, tool
Body formula is as follows：50mMol/l CB, 0.01% BSA, 0.4% S3 (Shanghai is prompt peaceful)；
Film process liquid B is that wherein PBs is molar concentration, and remaining is mass percent, and specific formula is as follows：10mMol/l's
PBs, 0.32% sucrose, 4% CNB5000 (Shanghai is prompt peaceful)；
Abbreviation：S3：One kind of surfactant；CNB5000：One kind of surfactant；BSA：Bovine serum albumin(BSA)；
CB：Carbonic acid buffer, containing sodium acid carbonate and sodium carbonate；PBs：Phosphate buffer, includes sodium dihydrogen phosphate, disodium hydrogen phosphate and chlorine
Change sodium；
(3) labelled reagent is prepared：CRP detects the latex fluorescence labeling of antibody 2, and control antibodies (chicken IgY) are also glimmering with latex
Signal, be stored in storing liquid (wherein Tris-cl be molar concentration, remaining is mass percent, and specific formula is as follows：
50mMol/l Tris-cl, 0.5%BSA, pH 7.8) it is standby.
(4) label band is prepared：By label by 5.0mg/ml prepare after, with BIODOT XYZ3060 spray film device by its
It is sprayed on nitrocellulose filter, dries (evaporation, vacuum, lyophilized) formation label band.Note：Label band, detection band and Quality Control
Putting in order for band is followed successively by label band, detection band and quality control band to climb water direction along sample.
(5) sample pad is prepared：Sample pad, in sample pad, is pre-processed with sample treatment liquid specking, is dried and (is evaporated, very
It is empty, lyophilized) it is standby.
Prescription for the treatment of liquid is：Wherein Tri-cl is molar concentration, and remaining is mass percent, and specific formula is as follows
50mMol/l Tris-CL, 0.5% Casein, 0.5% BSA, 0.1% Tween-20,0.05% PEG, 0.1%
Tween-80,0.05% PVP, 0.3% the sour sodium of two citric acid monohydrates, 2% sucrose.
Abbreviation：BSA：Bovine serum albumin(BSA), Casein：Casein, Tris-CL：Trishydroxymethylaminomethane hydrochloric acid, PEG：
Polyethylene glycol, Tween-20：Polysorbas20, Tween-80：Tween 80, PVP：Polyvinylpyrrolidone
Get adsorptive pads ready.
(6) assemble：Described sample pad, nitrocellulose filter and adsorptive pads are fixed on bottom plate and cut out with being sequentially connected
It is that 3-5mm produces described detecting system into width, described label band, detection band and quality control band is by sample pad to adsorptive pads
Direction is arranged in order.
Described detecting system is put into observation window (data being used on acquisition testing band and quality control band) and sample-adding
In the jam in hole (be used for testing sample to be added dropwise), by well to being loaded in sample pad, sample through climb water by label band,
Detection band and quality control band, it is observed that window collects detection band and data on quality control band and analyzes testing result.Draw 5 microlitres of samples
Product, with physiological saline as dilution (1000 microlitres), piping and druming repeatedly is mixed, is then added in jam well, label is contained in
On nitrocellulose filter.Due to the addition of sample, label dissolving, release, and measured object reacts in sample.Due to capillary
Lateral chromatography forward is acted on, data are read in 5 minutes.
Table 1
As can be seen from Table 1, when this method label consumption is less than 3 times of original recipe, detection line and matter in CRP projects
The fluorescent value of control line is both greater than original recipe, so label consumption saves a lot.100 times of this method Sample Dilution multiple, it is former
First 300 times of method.
The not most part of the utility model, those skilled in the art can as needed be selected according to existing knowledge, than
Such as, as needed can by latex fluorescence of selectable marker, time-resolved fluorescence, upper transfer be luminous, quantum dot fluorescence, fluorescence dye
Material or collaurum；Such as sequencing of mark detection band and quality control band etc..
It is described above, embodiment only of the present utility model, but protection domain of the present utility model do not limit to
In this, any one skilled in the art can readily occur in change in the technical scope that the utility model is disclosed
Or replace, it should all cover within protection domain of the present utility model.Therefore, protection domain of the present utility model should be with the power
The protection domain that profit is required is defined.
Claims (5)
1. a kind of immune lateral chromatography detecting system, it is characterised in that including：
Bottom plate, it includes nearly sample end and remote sample end；It is sequentially connected ground to remote sample end by nearly sample end on described bottom plate
It is provided with sample pad, nitrocellulose filter and adsorptive pads；Label band, described nitre are provided with described nitrocellulose filter
Acid cellulose film is the nitrocellulose filter by Seal treatment；Detection band is provided between described label band and adsorptive pads
And quality control band.
2. immune lateral chromatography detecting system according to claim 1, it is characterised in that contain on described label band
Label, described label is latex fluorescence, time-resolved fluorescence, luminous upper transfer, quantum dot fluorescence, fluorescent dye or glue
Body gold.
3. immune lateral chromatography detecting system according to claim 1, it is characterised in that contain inspection in described detection band
Antibody is surveyed, described detection antibody detects antibody for c reactive protein.
4. immune lateral chromatography detecting system according to claim 1, it is characterised in that on described quality control band containing pair
According to antibody, described control antibodies are goat-anti chicken IgY.
5. immune lateral chromatography detecting system according to claim 1, it is characterised in that the length of described detecting system
For 6-8cm, width is 3-6mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621147170.3U CN206573587U (en) | 2016-10-21 | 2016-10-21 | immune lateral chromatography detecting system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621147170.3U CN206573587U (en) | 2016-10-21 | 2016-10-21 | immune lateral chromatography detecting system |
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| Publication Number | Publication Date |
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| CN206573587U true CN206573587U (en) | 2017-10-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108918884A (en) * | 2018-06-08 | 2018-11-30 | 广州海孚医疗科技有限公司 | The immuno-chromatographic test paper strip and preparation method thereof of quantitative detection dog c reactive protein |
-
2016
- 2016-10-21 CN CN201621147170.3U patent/CN206573587U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108918884A (en) * | 2018-06-08 | 2018-11-30 | 广州海孚医疗科技有限公司 | The immuno-chromatographic test paper strip and preparation method thereof of quantitative detection dog c reactive protein |
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