CN108398554A - A kind of kit of anti-torch-IgM types antibody spectrum chip and preparation method thereof and TORCH detections - Google Patents
A kind of kit of anti-torch-IgM types antibody spectrum chip and preparation method thereof and TORCH detections Download PDFInfo
- Publication number
- CN108398554A CN108398554A CN201810188202.1A CN201810188202A CN108398554A CN 108398554 A CN108398554 A CN 108398554A CN 201810188202 A CN201810188202 A CN 201810188202A CN 108398554 A CN108398554 A CN 108398554A
- Authority
- CN
- China
- Prior art keywords
- torch
- igm
- chip
- quality control
- control point
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56905—Protozoa
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56927—Chlamydia
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56994—Herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5761—Hepatitis B
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/18—Togaviridae; Flaviviridae
- G01N2333/19—Rubella virus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/44—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from protozoa
- G01N2333/45—Toxoplasma
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
Abstract
The invention belongs to technical field of biological, a kind of anti-torch IgM types antibody spectrum chip and preparation method thereof and the TORCH kits detected and detection method are disclosed.Anti- torch IgM type antibody spectrum chip of the present invention can not only the related pathogenic microorganisms of more kinds of TORCH of simultaneous quantitative detection infection, and coating buffer and coating condition, closed stablity agent by optimizing chip, keep anti-torch IgM types antibody spectrum chip stability more preferable, service life is longer.The kit of TORCH detections of the present invention, can not only effectively reduce the usage amount of related antigen, reduce antigen cost, while keeping its stationary phase longer, and service life is longer, reduces transportation cost, improves the convenience of storage, transport.The infection of more kinds of related pathogenic microorganisms of TORCH detection methods energy simultaneous quantitative detection TORCH of the present invention, high sensitivity, specificity are good.
Description
Technical field
The invention belongs to technical field of biological, and in particular to a kind of anti-torch-IgM types antibody spectrum chip and its system
The kit of Preparation Method and TORCH detections.
Background technology
TORCH is the pathogen for leading to congenital intrauterine infection and perinatal infection and causing peri-natal infant deformity, it is one
The English name abbreviation of group pathogenic microorganism, wherein T (Toxoplasma) is toxoplasma, and O (Others) is the micro- life of other cause of diseases
Object, such as microspironema pallidum, herpes zoster virus, assays for parvovirus B 19, Coxsackie virus, R (Rubella.Virus) is rubella
Virus, C (Cytomegalo.Virus) are cytomegaloviruses, and H (Herpes.Virus) is herpe simplex I/II types.TORCH
One of an important factor for infection is serious harm Neonatal Health, is known as TORCH syndromes in perinatology, and infection is in generation
Criticality is distributed.In population of China, torch infection is widely present, and pregnant woman is after torch infection occurs for gestation without apparent clinical
Symptom, but after fetal infection, multiple organs such as fetus or neonatal liver, kidney, the heart, brain may be caused developmental defect and work(occur
Energy obstacle, it is possible to cause embryo/fetal abortion, premature labor, intrauterine growth retardation, deformity, stillborn foetus and neonatal death etc. bad
Consequence constitutes prenatal and postnatal care and population quality and greatly threatens.Therefore, the birth rate and raising to reduce Disabled children go out stranger
Mouthful quality, should pole carry out the serological screening of torch infection to find Disadvantage pregnancy and timely processing early.To newborn
TORCH detections should routinely be carried out, neonatal TORCH infection situation is understood, so as to early intervention, early treatment.
Four kinds of toxoplasma, rubella virus, cytomegalovirus, herpe simplex I/II types pathogenic microorganisms in the detection of TORCH
Infection account for about 90%, remaining 10% is other pathogenic microorganisms, including assays for parvovirus B 19, Coxsackie virus, treponemal
The infection such as body, hepatitis B, Chlamydia.At present to the detection method of TORCH mainly have ELISA, immunofluorescent test (IFT),
Colloidal gold and genetic chip etc..In China, most convenient, most common methods for screening are to use ELISA diagnostic techniques.
ELISA be detect human serum in specific IgM, IgM antibody, due to IgM be early infection index, on fetus influence it is huge
Greatly, so the detection of IgM is concerned, the detection of specific IgM is the reliable basis of diagnosing fetal intrauterine infection in placenta.
ELISA reagents are widely used in common lab due to its stabilization, high sensitivity, high specificity, the advantages that at low cost, but one
As be used for doing qualitative, cannot quantify.And IFT is generally just for the detection of single index, the method for colloidal gold is generally also only fixed
Property or sxemiquantitative, of high cost although biochip technology effect is good, price general charged is expensive.
Invention content
In view of this, the present invention for the defects in the prior art, provides a kind of anti-torch-IgM types antibody spectrum chip
And preparation method thereof with TORCH detection kit.
To achieve the purpose of the present invention, the present invention adopts the following technical scheme that.
A kind of anti-torch-IgM types antibody spectrum chip, is coated with the egg that TORCH detects the antigen of relevant pathogenic microorganism
White chip.
The present invention uses protein biochip technology, and the multiple pathogens of TORCH are integrated on protein chip and are prepared for height
The TORCH associated antibodies IgM chips that flux, more Testing index, performance are stable, reproducible, accuracy is high.
Wherein, preferably, it is toxoplasma (TOX), cytomegalovirus that the TORCH, which detects relevant pathogenic microorganism,
(CMV), rubella virus (RV), herpes simplex virus (HSV), Human parvovirus B19, Coxsackie virus, microspironema pallidum, second
At least one of hepatovirus (HBV), Chlamydia.
In some embodiments, it is toxoplasma (TOX), giant cell disease that the TORCH, which detects relevant pathogenic microorganism,
Malicious (CMV), rubella virus (RV), herpes simplex virus (HSV), Human parvovirus B19, Coxsackie virus, microspironema pallidum,
Hepatitis B (HBV) and Chlamydia, totally 9 kinds of the relevant pathogenic microorganisms of TORCH.Wherein herpes simplex virus (HSV) includes single
Pure herpesviral-I types, herpes simplex virus-II types.
Anti- torch-IgM types antibody spectrum chip of the present invention, the protein chip also include Quality Control point and/or reference
Point.
Wherein, the Quality Control point includes at least one positive quality control point (PC), at least one negative Quality Control point (NC), at least
One sample Quality Control point (SC) and/or at least one enzyme mark Quality Control point (EC).The reference point includes that the reference of various concentration is bent
Line point (S1-S5) and/or at least one chip position reference point (Loc).
In some embodiments, chip of the present invention includes a positive quality control point (PC), a negative Quality Control point
(NC), a sample Quality Control point (SC), an enzyme mark Quality Control point (EC), 5 reference curve points (S1-S5) and a chip position
Reference point (Loc).
In some embodiments of the invention, the positive quality control point can be people IgM, the enzyme linked immunological marker used
It is the enzyme mark of anti-human IgM.In some other embodiments, the positive quality control point can also be coated with BSA-DNP conjugates, make
Enzyme linked immunological marker is exactly the mixed liquor that enzyme marks the enzymic-labelled antibody of anti-human IgM and anti-DNP-BSA.
In some embodiments of the invention, the negative Quality Control point can be less than the micro-concentrations of reaction signal value
People IgM.It can also be substituted with other unrelated proteins in some other embodiments.
In some embodiments of the invention, sample Quality Control point can be the anti-human IgM of mouse.In some other embodiments
In other anti-human IgM can also be used.
In some embodiments of the invention, the enzyme mark Quality Control point can be people IgM.In some other embodiments
The antibody (enzyme mark is rabbit-anti people IgM) of other anti-rabbit, such as goat-anti rabbit IgM antibody can also be used.
In some embodiments of the present invention, the reference curve point is the people IgM of five kinds of various concentrations.Such as 0.5 μ g/ml, 1
The human IgG of μ g/ml, 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml.
In the present invention, it is people's IgM solution of 2 μ g/ml that the position reference point of described chip itself is coated, and main is exactly pair
Positioning action when array value.
The present invention also provides the preparation methods of the anti-torch-IgM types antibody spectrum chip, with coating buffer solution dilution
TORCH detects the antigen of relevant pathogenic microorganism, Quality Control point albumen and/or reference point albumen, by point sample instrument with lattice array
Form is coated in chip matrix.
TORCH is detected antigen and the institute of relevant 10 kinds of pathogenic microorganisms by the point sample instrument of high-precision by the present invention
Quality Control point albumen, the reference point albumen needed is coated in the form of 4 × 5 lattice array in chip matrix
Wherein, the coating buffer solution is the buffer solution containing PEG, water soluble Beta-cyclodextrin, trehalose, preservative and glycerine.
In some embodiments, the caching liquid is the Tris buffer solutions or pH7.4- of the CB buffer solutions of pH9.6, pH8.5
7.6 PBS buffer solution.
Contain PEG in coating buffer solution of the present invention.In some embodiments, the PEG is PEG4000.It is described
The content of PEG is preferably 0.5%~1%, and more preferably 0.5%.
Contain water soluble Beta-cyclodextrin in coating buffer solution of the present invention so that more stable, uniform, the coated point of coating is more
It is regular, more round, CV smallers.Preferably, the water soluble Beta-cyclodextrin is 0.01%~0.02%Captisol, 0.02%-
0.04% 2- hydroxy-beta-cyclodextrins or carboxymethyl-beta-cyclodextrin;
Contain trehalose in coating buffer solution of the present invention.Preferably, the content of the trehalose is 5%~8%;
Contain preservative in coating buffer solution of the present invention.In some embodiments, the preservative is
Proclin300.Preferably, the content of the preservative is 0.05%.
Contain glycerine in coating buffer solution of the present invention.Preferably, the content of the glycerine is 15%.
Preferably, coating described in the preparation method of chip of the present invention is 2-8 DEG C, coating 16-24h is stood.
Chip matrix of the present invention includes but not limited to for enzyme reaction plate, sheet glass, chemical films, Bio-sil.Its
It is suitble to the carrier of protein attachment to can also be used as chip matrix.
Further, the preparation method of chip of the present invention further includes the steps that being closed with closed stablity agent.
Preferably, the closed stablity agent is mannitol containing 1%-4%, PVP40000,1%-3% of 2%-5%
The 0.01M disodium phosphate solns of the pH7.4 of NH2-PEG, 0.05%NaN3.In some embodiments, the closed stablity agent
For 1% mannitol, 2% PVP40000,1%NH2The 0.01M disodium phosphate solns of the pH7.4 of-PEG, 0.05%NaN3.
The closing is preferably that room temperature closes 1h.
The present invention also provides a kind of kits of TORCH detections, including the anti-torch-IgM types antibody repertoire core
Piece.
Further, the kit of TORCH detection further include enzyme marker, enzyme mark dilution stabilizer, sample diluting liquid,
At least one of cleaning solution, developing solution.In some embodiments, the kit of TORCH detection further include enzyme marker,
Enzyme mark dilutes stabilizer, sample diluting liquid, cleaning solution and developing solution.
Preferably, the enzyme marker is the anti-human IgM antibodies of horseradish peroxidase-labeled, as goat-anti people IgM is anti-
Body.
Preferably, the enzyme mark dilution stabilizer is the cow's serum γ ball eggs of the citric acid comprising 0.05M, 3%-5%
In vain, the glycine betaine of the gum arabic 1%-2% of PEG10000,0.05%-0.2% of 2%-4%, 0.05%
The Tris solution of the 100mM of the pH7.4 of Proclin3000.It is furthermore preferred that the enzyme mark dilution stabilizer is to include 0.05M's
Citric acid, 3% cow's serum gamma Globulin, 2% PEG10000,0.05% gum arabic 1% glycine betaine, 0.05%
Proclin3000 pH7.4 100mM Tris solution.
Preferably, the sample diluting liquid is comprising 0.15M NaCl, 0.05%Tween20,0.01% casein
The 0.02M Tris solution of pH7.4.
Preferably, the cleaning solution is the 0.02M Tris of the pH7.4 comprising 0.15M NaCl, 0.05%Tween20
Solution.
Preferably, the developing solution is sedimentation type TMB.
The present invention also provides a kind of TORCH detection methods, sample to be tested is added described after being diluted with sample diluting liquid
On anti-torch-IgM types antibody spectrum chip, enzyme marker is added, developing solution is added and is protected from light colour developing, fluorescence detection device detection knot
Fruit calculates the anti-torch-IgM types antibody signal value in sample to be tested.
Further, cleaning solution is washed after the detection method includes incubating before enzyme marker is added and developing solution is added
The step of washing.
Detection method of the present invention detects TORCH in subject's sample by the immunology principle of antigen-antibody reaction
The level of the associated antibodies IgM of infection.The signal detection system of detection method of the present invention is chemiluminescent mode, can be used
Substrate is the chemiluminescent substrate of such as luminol, and the reading of result is carried out by fluorescence detection device or instrument.
As shown from the above technical solution, the present invention provides a kind of anti-torch-IgM types antibody spectrum chip and its preparation sides
The kit and detection method of method and TORCH detections.Anti- torch-IgM types antibody spectrum chip of the present invention, is coated with TORCH
Detect the protein chip of the antigen of relevant pathogenic microorganism.Also include further Quality Control point and/or reference point.It is described anti-
Torch-IgM type antibody spectrum chips detect the antigen of relevant pathogenic microorganism with coating buffer solution dilution TORCH, egg is ordered in Quality Control
White and/or reference point albumen, is coated in the form of lattice array in chip matrix by point sample instrument, further uses closed stablity agent
Closing.It can not only determine simultaneously compared to traditional enzyme linked immunological, colloidal gold detection and existing biochip technology, the present invention
The infection of amount detection more kinds of related pathogenic microorganisms of TORCH, and coating buffer and coating condition, closing by optimizing chip are steady
Determine agent, keeps anti-torch-IgM types antibody spectrum chip stability more preferable (2-8 DEG C refrigerates 2 years, and room temperature can store 8 months), use week
Phase is longer.The kit of TORCH detections of the present invention, including the anti-torch-IgM types antibody spectrum chip and enzyme mark
Remember at least one of object, enzyme mark dilution stabilizer, sample diluting liquid, cleaning solution, developing solution.The kit can not only effectively drop
The usage amount of low related antigen reduces antigen cost, while optimizing kit, keeps its stationary phase longer, 2-8 DEG C can place two
Year, room temperature can place 8 months, and the overall performance of kit is more preferable, and service life is longer, can also further decrease transportation cost,
Improve the convenience of storage, transport.More kinds of related cause of diseases of TORCH detection methods energy simultaneous quantitative detection TORCH of the present invention
The infection of microorganism, high sensitivity, specificity is good, and a kind of efficient, efficiently detection means newly is provided for prenatal and postnatal care.
Specific implementation mode
The invention discloses the reagents that a kind of anti-torch-IgM types antibody spectrum chip and preparation method thereof is detected with TORCH
Box.Those skilled in the art can use for reference present disclosure, be suitably modified technological parameter realization.In particular, it should be pointed out that all
Similar replacement and change is apparent to those skilled in the art, they are considered as being included in the present invention.This
The method and product of invention are described by preferred embodiment, and related personnel can obviously not depart from the present invention
Hold, method described herein is modified or is suitably changed and is combined in spirit and scope, to realize and apply skill of the present invention
Art.
For a further understanding of the present invention, below in conjunction with the embodiment of the present invention, to the technical side in the embodiment of the present invention
Case is clearly and completely described, it is clear that and described embodiments are only a part of the embodiments of the present invention, rather than all
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art institute without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
Unless otherwise specified, reagent involved in the embodiment of the present invention is commercial product, can pass through business canal
Road purchase obtains.Wherein, the content of BSA is mass volume ratio, based on g/ml, if 5%BSA is to contain 5gBSA in 100ml PBS;
The content of Tween-20 is volume ratio, and such as 0.05% Tween-20 is that 1ml PBS contain 0.5 μ l Tween-20s;The content matter of PEG4000
Volume ratio is measured, based on g/ml, if 0.5%PEG is PEG4000 containing 0.5g in 100ml buffer solutions;Trehalose is mass volume ratio,
Based on g/ml, if 5% trehalose is trehalose containing 5g in 100ml buffer solutions;Captisol is mass volume ratio, based on g/ml,
Captisol such as 0.02% is to contain 0.02gCaptisol in 100ml buffer solutions;Proclin300 is mass volume ratio, by g/
Ml is counted, and the Proclin300 such as 0.05% is Proclin300 containing 0.05g in 100ml buffer solutions.In addition, PVA, glycine betaine,
NaN3, P-hydroxybenzoic acid sodium, gum arabic, casein are mass volume ratio, based on g/ml.
The preparation of embodiment 1, anti-torch-IgM types antibody repertoire chip agent box
1, the coating of antigen:
1) the specific distribution of the Array Design of chip and antigen and reference point (is not limited only to following arrangement to set as follows
Meter):
2), specifically it is coated with process:
First press the dilution antigen and coherent reference point albumen:
PC, NC, S1, S2, S3, S4, S5, EC point in array coated respectively is 2 μ g/ml, 0.01 μ g/ml, 0.5 μ g/
The people IgM of ml, 1 μ g/ml, 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 2.5 μ g/ml, dilution buffer are the CB buffer solutions of pH9.6
(wherein contain 0.5% PEG4000,5% trehalose, 0.02% Captisol, 0.05% Proclin300, and
15% glycerine).
It is the goat-anti IgM antibody of 2 μ g/ml that SC points are coated, and dilution buffer is the CB buffer solutions of pH9.6.
It is the people IgM of 2 μ g/ml that Loc points are coated, and dilution buffer is the CB buffer solutions of pH9.6.
The dilution of envelope antigen:50 μ g/ml, HSV-I recombinant antigen of Tox purified antigens, 20 μ g/ml, HSV-II recombinant antigens
20 μ g/ml, RV recombinant antigen, 15 μ g/ml, CMV recombinant antigen, 10 μ g/ml, HBV antigen, 8 μ g/ml, TP recombinant antigen, 40 μ g/ml,
25 μ g/ml, CV recombinant antigen of B19 recombinant antigens, 30 μ g/ml, CT recombinant antigen, 25 μ g/ml.
By the antigen diluted respectively with 0.22 μm of membrane filtration, then array is carried out with BioDot precisions point sample instrument
Coating.After whole arrays complete point sample, chip is placed in 2-8 DEG C, is coated with 16-24h.
2, it closes:
Coated chip is taken out, is cleaned 3 times with the PBST cleaning solutions of pH7.4, the closed stablity of 150 μ l is then added per hole
Agent (1% mannitol, 2% PVA40000,1%NH2-PEG, 0.05%NaN3 preservative pH7.4 disodium phosphate soln),
Room temperature closes 1h, then pats dry, and in humidity 15% hereinafter, being placed at room temperature for, is sealed after dry 4h, 2-8 DEG C of preservation.
3, enzyme marking reagent is prepared
With enzyme mark dilution stabilizer (Tris of 100mM, wherein the citric acid containing 50mM, 3% cow's serum gamma Globulin,
2% PEG10000,0.05% gum arabic 1% glycine betaine and 0.05% Proclin300) by horseradish peroxide
The rabbit-anti human IgM antibody of compound enzyme label is diluted to 6000 times.
4, the foundation of the reaction system of kit
Take out chip reagent, balance to room temperature;
Sample-adding:By negative and positive control serum and with sample diluting liquid (0.02M Tris, 0.15M NaCl,
0.05%Tween20,0.01% casein, pH7.4) 101 times of sample to be tested is diluted, chip to be measured is added per 100 μ l of hole
It is reacted in hole.
It incubates:React at room temperature 30min.Add 300 μ l cleaning solutions (0.02M Tris, 0.15M NaCl, 0.05%Tween20,
PH7.4), wash 3 times, each 1min.
Add ELIAS secondary antibody (enzyme marker):50 μ l enzyme labelled antibodies are added per hole.
It incubates:React at room temperature 30min.Add 300 μ l cleaning solutions, washs 3 times, each 1min.
Colour developing:50 μ l of TMB color developing agents are added per hole, room temperature is protected from light 30min.
It measures:In 30min, the signal value that each reacting hole corresponds to antibody is read and calculated with detector.
Embodiment 2, anti-torch-IgM types antibody repertoire chip agent box evaluation of the accuracy
1, anti-with the 10 determining resisting toxoplasmosis IgM positive serums and 10 of Diasorin companies ELISA kit screening
Toxoplasma Gondi IgM negative serum, the anti-torch-IgM types antibody spectrum chip prepared with embodiment 1 are tested, as a result such as the following table 1
(+indicate is positive ,-indicate negative).
Anti- torch-IgM types antibody repertoire chip testing resisting toxoplasmosis IgM serum results prepared by 1 embodiment 1 of table
2, with 10 determining rubella virus IgM positive serums of Diasorin companies ELISA kit screening and 10
Rubella virus IgM negative serums, the anti-torch-IgM types antibody repertoire chip testing prepared with embodiment 1, as a result such as the following table 2
(+indicate is positive ,-indicate negative).
Anti- torch-IgM types antibody repertoire chip testing rubella virus IgM serum results prepared by 2 embodiment 1 of table
3, with 10 determining anti-cytomegalovirus IgM positive serums and 10 of Diasorin companies ELISA kit screening
Example anti-cytomegalovirus IgM negative serums, anti-torch-IgM types antibody spectrum chip prepared by embodiment 1 are tested, as a result such as
The following table 3 (+indicate positive ,-indicate negative).
Anti- torch-IgM types antibody repertoire chip testing anti-cytomegalovirus IgM serum results prepared by 3 embodiment 1 of table
4, with 10 determining anti-herpes simplex virus-I IgM positive serums of Diasorin companies ELISA kit screening
With 10 herpes simplex virus-I IgM negative serums, the anti-torch-IgM types antibody repertoire chip testing prepared with embodiment 1,
As a result such as the following table 4 (+expression is positive, and-expression is negative).
Anti- torch-IgM types antibody repertoire chip testing anti-herpes simplex virus-I IgM serum knots prepared by 4 embodiment 1 of table
Fruit
5, with 10 determining anti-herpes simplex virus-II IgM positive bloods of Diasorin companies ELISA kit screening
Cleer and peaceful 10 anti-herpes simplex virus-II IgM negative serums, embodiment 1 prepare anti-torch-IgM types antibody spectrum chip into
It goes and tests, as a result such as the following table 5 (+expression is positive ,-expression feminine gender).
Anti- torch-IgM types antibody repertoire chip testing anti-herpes simplex virus-II IgM serum prepared by 5 embodiment 1 of table
As a result
6, determine that anti-parvovirus B1 IgM sun is infected in infection with 10 of EUROIMMUN companies ELISA kit screening
Property serum and 10 anti-parvovirus B1 IgM negative serums, the anti-torch-IgM types antibody spectrum chip prepared with embodiment 1 into
It goes and tests, as a result such as the following table 6 (+expression is positive ,-expression feminine gender).
The anti-parvovirus B1IgM serum results of anti-torch-IgM types antibody repertoire chip testing prepared by 6 embodiment 1 of table
7, with the anti-Coxsackie virus IgM positive serums of 10 determinations and 10 of EUROIMMUN companies ELISA kit screening
The anti-Coxsackie virus IgM negative serums of example, the anti-torch-IgM types antibody spectrum chip prepared with embodiment 1 are tested, as a result
Such as the following table 7 (+expression is positive, and-expression is negative).
The anti-Coxsackie virus IgM serum results of anti-torch-IgM types antibody repertoire chip testing prepared by 7 embodiment 1 of table
8, with anti-microspironema pallidum (TP) the IgM positive serums of 10 determinations of EUROIMMUN companies ELISA kit screening
With 10 anti-microspironema pallidum (TP) IgM negative serums, the anti-torch-IgM types antibody spectrum chip prepared with embodiment 1 carries out
Test, as a result such as the following table 8 (+expression is positive, and-expression is negative).Anti- torch-IgM types antibody spectrum chip prepared by 8 embodiment 1 of table
Test anti-microspironema pallidum IgM serum results
9, with 10 determining anti-hepatitis virus (HBV) core antibody IgM sun of Diasorin companies ELISA kit screening
Property serum and 10 anti-HBcIgM negative serums, the anti-torch-IgM types antibody repertoire prepared with embodiment 1
Chip is tested, as a result such as the following table 9 (+expression is positive, and-expression is negative).Anti- torch-IgM types prepared by 9 embodiment 1 of table are anti-
Body spectrum chip tests anti-HBcIgM serum result
10, with 10 determining Against Chlamydia Trachomatis (CT) IgM positive serums and 10 of IBL companies ELISA kit screening
Example Against Chlamydia Trachomatis (CT) IgM negative serums, the anti-torch-IgM types antibody spectrum chip prepared with embodiment 1 are tested,
As a result such as the following table 10 (+expression is positive, and-expression is negative).
Anti- torch-IgM types antibody repertoire chip testing Against Chlamydia Trachomatis IgM serum results prepared by 10 embodiment 1 of table
In conclusion anti-torch-IgM types antibody repertoire chip testing items antipathogen IgM's prepared by the present invention is accurate
Property meet the requirements, accuracy is high.
Embodiment 3, anti-torch-IgM types antibody repertoire chip agent box Evaluation on specificity
Anti- torch-IgM types antibody repertoire chip agent box and traditional ELISA kits more of the present invention are special.With bow
For shape worm project, 20 resisting toxoplasmosis IgM antibody clinic negative serums are chosen, the anti-torch-IgM types prepared with embodiment 1
Antibody spectrum chip and Diasorin companies ELISA kit are carried out at the same time test, test data such as the following table 11 ("+" indicates the positive,
" ± " table is that weak positive "-" indicates negative).
Anti- torch-IgM types antibody spectrum chip specificity comparison result prepared by 11 embodiment 1 of table
The results show that anti-20 negative serums of torch-IgM types antibody repertoire chip testing prepared by embodiment 1 do not occur
False sun, and Diasorin companies ELISA kit in the market tests 20 negative serums and 2 official holidays sun occurs.Show the present invention
The anti-torch-IgM types antibody spectrum chip specificity is more preferable compared with Diasorin companies ELISA kit.
The estimation of stability of embodiment 4, anti-torch-IgM types antibody repertoire chip agent box
Embodiment 1 is prepared into anti-torch-IgM types antibody repertoire chip agent box and is individually positioned in 2-8 DEG C after 0 month, 6
After month, after 12 months, after 18 months, 24 months, be placed on 18-28 DEG C after 0 month, after 2 months, after 4 months, after 6 months, 8
Test signal Value Data is distinguished with anti-corresponding antigens IgM quality controlled serums after a month, as a result such as table 12 and 13.
12 embodiment 1 of table prepares anti-2-8 DEG C of estimation of stability result of torch-IgM types antibody repertoire chip agent box
13 embodiment 1 of table prepares anti-18-28 DEG C of estimation of stability result of torch-IgM types antibody repertoire chip agent box
The results show that anti-torch-IgM types antibody repertoire chip agent box of the present invention is 2 years in 2-8 DEG C of stationary phase,
It it is 1 year in 18-28 DEG C of stationary phase.
Closed stablity agent and enzyme mark dilution are to stability in embodiment 5, anti-torch-IgM types antibody repertoire chip agent box
Influence
It is closed with using general in the anti-torch-IgM types antibody repertoire chip agent box prepared using the embodiment of the present invention 1
Liquid (pH=7.4 0.01M PBS contain 5%BSA), (pH=7.4 0.01M PBS are containing 0.05% Tween-20,1% for enzyme mark dilution
BSA) the anti-torch-IgM types antibody spectrum chip contrast agent box (kit other methods step is with embodiment 1) formed, respectively
Be placed on 2-8 DEG C after 6 months, after 12 months, after 18 months, 24 months, after being placed on 18-28 DEG C, 2 months, after 4 months, 6
Test signal Value Data is distinguished after month, after 8 months with anti-corresponding antigens IgM quality controlled serums, as a result such as table 14 and 15.
The 2-8 DEG C of closed stablity agent of table 14, enzyme mark dilute stabilizer estimation of stability result
The 18-28 DEG C of closed stablity agent of table 15, enzyme mark dilute stabilizer estimation of stability result
The results show that preparing anti-torch-IgM types antibody spectrum chip detection examination using general confining liquid and enzyme mark dilution
After 2-8 DEG C of agent box is placed 12 months or 18-28 DEG C place 2 months after using corresponding quality controlled serum test signal intensity it is apparent under
Drop, and the anti-torch-IgM types antibody repertoire chip agent box of the present invention places 2-8 DEG C and places placement 2 in 12 months or 18-28 DEG C
After month good performance is also shown with the test of corresponding quality controlled serum.Show the anti-torch-IgM types antibody spectrum chip of the present invention
Closed stablity agent, enzyme mark dilution stabilizer in detection kit is better than general confining liquid and enzyme mark dilution, can be largely
Improve the stability of product.
Embodiment 6, anti-torch-IgM types antibody repertoire chip agent box and antigen cost used in colloidal gold kit
Compare
Compared with colloidal gold fast detecting test paper disclosed in the Chinese patent application No. is 201110220541 antigen at
This:
Antigen dosage used in fluorescence immune chromatography kit calculates:
By taking toxoplasma antigen is coated with as an example:Toxoplasma antigen peridium concentration 1.5mg/ is mentioned in CN201110220541 patents
Ml, by 1 μ l/cm coatings, each wide 0.4cm of test strips, therefore the average dosage per person-portion antigen is about 0.6 μ g.
Antigen dosage used in kit of the present invention calculates:
The present invention uses BioDot precision point sample instruments to carry out the coating of antigen, and the coating volume each put is only
10nL, therefore the amount for being coated with antigen used in every person-portion is:Toxoplasma purified antigen dosage:50 μ g/ml × 10nL × 10-6=
0.005μg。
It follows that the usage amount of toxoplasma antigen will obviously be less than 2 numbers of colloidal gold fast detecting test paper in the present invention
Magnitude.Other antigen dosage situations are similarly.In conclusion showing anti-torch-IgM types antibody spectrum chip of the present invention
Kit can effectively reduce the usage amount of related antigen, while the sensitivity and specificity for ensureing kit, reduce phase
The antigen use cost of pass.
Claims (13)
1. a kind of anti-torch-IgM types antibody spectrum chip, is coated with the albumen that TORCH detects the antigen of relevant pathogenic microorganism
Chip.
2. chip according to claim 1, the TORCH detect relevant pathogenic microorganism be toxoplasma (TOX), it is big and small
Cellular virus (CMV), rubella virus (RV), herpes simplex virus (HSV), Human parvovirus B19, Coxsackie virus, syphilis spiral shell
Revolve at least one of body, hepatitis B (HBV), Chlamydia.
3. chip according to claim 1 or 2, the protein chip also includes Quality Control point and/or reference point;The Quality Control
Point comprising at least one positive quality control point (PC), at least one negative Quality Control point (NC), at least one sample Quality Control point (SC) and/
Or at least one enzyme mark Quality Control point (EC);The reference point includes the reference curve point (S1-S5) and/or at least one of various concentration
A chip position reference point (Loc).
4. chip according to claim 3, the positive quality control point coating people IgM or coating BSA-DNP conjugates;It is described
Negative Quality Control point coating less than the people IgM of the micro-concentrations of reaction signal value or other and TORCH detection pathogenic microorganisms without
The albumen of pass;The sample Quality Control point is to be coated with anti-human IgM;The antibody of the enzyme mark Quality Control point coating people IgM or anti-rabbit;It is described
Reference curve point is coated with the people IgM of various concentration;People's IgM solution of the chip position reference point coating known concentration.
5. the preparation method of the chip described in claim 1-4 any one, relevant with coating buffer solution dilution TORCH detections
Antigen, Quality Control point albumen and/or the reference point albumen of pathogenic microorganism, chip base is coated in by point sample instrument in the form of lattice array
In matter.
6. preparation method according to claim 5, the coating buffer solution be containing PEG, water soluble Beta-cyclodextrin, trehalose,
The buffer solution of preservative and glycerine;The coating is 2-8 DEG C, stands coating 16-24h;The chip matrix be enzyme reaction plate,
Sheet glass, chemical films, Bio-sil.
7. preparation method according to claim 6, the caching liquid is the Tris bufferings of the CB buffer solutions of pH9.6, pH8.5
The PBS buffer solution of liquid or pH7.4-7.6;The PEG is PEG4000;The content of the PEG is 0.5%~1%;It is described water-soluble
Property cyclodextrin be 0.01%~0.02%Captisol, the 2- hydroxy-beta-cyclodextrins of 0.02%-0.04% or carboxymethyl-β-ring
Dextrin;The content of the trehalose is 5%~8%;The preservative is Proclin300;The content of the preservative is
0.05%;The content of the glycerine is 15%~20%.
Further include the steps that being closed with closed stablity agent 8. according to the preparation method described in claim 5-7 any one.
9. preparation method according to claim 8, the closed stablity agent is mannitol containing 1%-4%, 2%-5%'s
PVP40000,1%-3%NH2The 0.01M disodium phosphate solns of the pH7.4 of-PEG, 0.05%NaN3.
10. a kind of kit of TORCH detections, including the anti-torch-IgM types antibody repertoire described in claim 1-4 any one
Chip.
11. kit according to claim 10 further includes enzyme marker, enzyme mark dilution stabilizer, sample diluting liquid, washes
Wash at least one of liquid, developing solution.
12. kit according to claim 11, the enzyme marker is that the anti-human IgM of horseradish peroxidase-labeled is anti-
Body;It is the citric acid comprising 0.05M, the cow's serum gamma Globulin of 3%-5%, 2%-4% that enzyme mark, which dilutes stabilizer,
The pH7.4 of the glycine betaine of the gum arabic 1%-2% of PEG10000,0.05%-0.2%, 0.05% Proclin3000
The Tris solution of 100mM;Sample diluting liquid is the pH7.4 comprising 0.15M NaCl, 0.05%Tween20,0.01% casein
0.02M Tris solution;Cleaning solution is the 0.02M Tris solution of the pH7.4 comprising 0.15M NaCl, 0.05%Tween20;
The developing solution is sedimentation type TMB.
13. a kind of TORCH detection methods, claim 1-4 any one institute is added after being diluted with sample diluting liquid in sample to be tested
On the anti-torch-IgM types antibody spectrum chip stated, enzyme marker is added, developing solution is added and is protected from light colour developing, fluorescence detection device inspection
It surveys as a result, calculating the anti-torch-IgM types antibody signal value in sample to be tested.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810188202.1A CN108398554B (en) | 2018-03-07 | 2018-03-07 | anti-TORCH-IgM antibody spectrum chip, preparation method thereof and TORCH detection kit |
PCT/CN2018/092768 WO2019169792A1 (en) | 2018-03-07 | 2018-06-26 | Anti-torch-igm antibody repertoire chip, preparation method therefor and torch detection kit |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810188202.1A CN108398554B (en) | 2018-03-07 | 2018-03-07 | anti-TORCH-IgM antibody spectrum chip, preparation method thereof and TORCH detection kit |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108398554A true CN108398554A (en) | 2018-08-14 |
CN108398554B CN108398554B (en) | 2020-08-07 |
Family
ID=63092558
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810188202.1A Active CN108398554B (en) | 2018-03-07 | 2018-03-07 | anti-TORCH-IgM antibody spectrum chip, preparation method thereof and TORCH detection kit |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN108398554B (en) |
WO (1) | WO2019169792A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111208298A (en) * | 2020-02-11 | 2020-05-29 | 潍坊市康华生物技术有限公司 | 2019 novel coronavirus antibody detection kit and preparation method thereof |
CN112213483A (en) * | 2020-09-18 | 2021-01-12 | 迪瑞医疗科技股份有限公司 | Chemiluminescence immunoassay kit for detecting herpes simplex virus 1+2 IgM antibody and preparation method thereof |
CN112748242A (en) * | 2020-12-21 | 2021-05-04 | 珠海碳云智能科技有限公司 | Secondary antibody buffer confining liquid for polypeptide chip technology platform detection, kit comprising same and application thereof |
WO2021134304A1 (en) * | 2019-12-30 | 2021-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | Kit, method and immunoassay analyzer for screening torch infection |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1330271A (en) * | 2001-07-12 | 2002-01-09 | 上海晶泰生物技术有限公司 | Protein-chip for prenated diagnosis and its preparing process |
CN1595158A (en) * | 2003-09-12 | 2005-03-16 | 上海前沿生物技术有限公司 | Kit for detecting four monstrum causing diseases simultaneously and method for preparing same |
US20100022407A1 (en) * | 1998-02-04 | 2010-01-28 | Life Technologies Corporation | Microarrays and uses therefor |
CN101769926A (en) * | 2008-12-30 | 2010-07-07 | 上海裕隆生物科技有限公司 | Integrated test reaction plate of five indicators of prenatal and postnatal care and kit |
CN103616507A (en) * | 2013-12-11 | 2014-03-05 | 山东博科生物产业有限公司 | Protein-free coated plate confining liquid |
CN103642781A (en) * | 2013-12-12 | 2014-03-19 | 山东博科生物产业有限公司 | Horse radish peroxidase protective agent |
CN105393119A (en) * | 2013-07-30 | 2016-03-09 | 生物辐射实验室股份有限公司 | Multiplex blocker beads for immunoassays |
CN105606826A (en) * | 2016-02-05 | 2016-05-25 | 中国农业大学 | Kit for detecting avian chlamydia psittaci by enzyme linked immunosorbent assay |
CN107615070A (en) * | 2015-05-29 | 2018-01-19 | 京瓷株式会社 | Detection method and detection means |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1412557A (en) * | 2001-10-11 | 2003-04-23 | 南京集慧科技开发有限责任公司 | ToRCH protein chip and its preparation method |
CN1414389A (en) * | 2002-08-19 | 2003-04-30 | 上海华冠生物芯片有限公司 | HCV and TORCH protein chip and its preparation and application method |
CN103808928A (en) * | 2014-02-12 | 2014-05-21 | 严银芳 | Method for preparing ToRCH-ELISA (Enzyme Linked Immunosorbent Assay Kit) diagnosis reagent |
-
2018
- 2018-03-07 CN CN201810188202.1A patent/CN108398554B/en active Active
- 2018-06-26 WO PCT/CN2018/092768 patent/WO2019169792A1/en active Application Filing
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100022407A1 (en) * | 1998-02-04 | 2010-01-28 | Life Technologies Corporation | Microarrays and uses therefor |
CN1330271A (en) * | 2001-07-12 | 2002-01-09 | 上海晶泰生物技术有限公司 | Protein-chip for prenated diagnosis and its preparing process |
CN1595158A (en) * | 2003-09-12 | 2005-03-16 | 上海前沿生物技术有限公司 | Kit for detecting four monstrum causing diseases simultaneously and method for preparing same |
CN101769926A (en) * | 2008-12-30 | 2010-07-07 | 上海裕隆生物科技有限公司 | Integrated test reaction plate of five indicators of prenatal and postnatal care and kit |
CN105393119A (en) * | 2013-07-30 | 2016-03-09 | 生物辐射实验室股份有限公司 | Multiplex blocker beads for immunoassays |
CN103616507A (en) * | 2013-12-11 | 2014-03-05 | 山东博科生物产业有限公司 | Protein-free coated plate confining liquid |
CN103642781A (en) * | 2013-12-12 | 2014-03-19 | 山东博科生物产业有限公司 | Horse radish peroxidase protective agent |
CN107615070A (en) * | 2015-05-29 | 2018-01-19 | 京瓷株式会社 | Detection method and detection means |
CN105606826A (en) * | 2016-02-05 | 2016-05-25 | 中国农业大学 | Kit for detecting avian chlamydia psittaci by enzyme linked immunosorbent assay |
Non-Patent Citations (4)
Title |
---|
乐爱平 等: "生物蛋白芯片技术及其在ToRCH综合症诊治中的应用于展望", 《江西医学检验》 * |
何文军 等: "反向蛋白质微点阵对怀孕女性TORCH感染的检测", 《国际检验医学杂志》 * |
印莉萍 等: "《分子细胞生物学实验技术》", 31 December 2001 * |
吴兴安 等: "《医学微生物学实验教程》", 31 December 2013 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021134304A1 (en) * | 2019-12-30 | 2021-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | Kit, method and immunoassay analyzer for screening torch infection |
CN111208298A (en) * | 2020-02-11 | 2020-05-29 | 潍坊市康华生物技术有限公司 | 2019 novel coronavirus antibody detection kit and preparation method thereof |
CN111208298B (en) * | 2020-02-11 | 2023-10-27 | 山东康华生物医疗科技股份有限公司 | 2019 novel coronavirus antibody detection kit and preparation method thereof |
CN112213483A (en) * | 2020-09-18 | 2021-01-12 | 迪瑞医疗科技股份有限公司 | Chemiluminescence immunoassay kit for detecting herpes simplex virus 1+2 IgM antibody and preparation method thereof |
CN112748242A (en) * | 2020-12-21 | 2021-05-04 | 珠海碳云智能科技有限公司 | Secondary antibody buffer confining liquid for polypeptide chip technology platform detection, kit comprising same and application thereof |
Also Published As
Publication number | Publication date |
---|---|
CN108398554B (en) | 2020-08-07 |
WO2019169792A1 (en) | 2019-09-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108398554A (en) | A kind of kit of anti-torch-IgM types antibody spectrum chip and preparation method thereof and TORCH detections | |
CN108398564A (en) | A kind of kit of anti-torch-IgG types antibody spectrum chip and preparation method thereof and TORCH detections | |
CN111413496B (en) | Novel coronavirus IgM/IgG antibody chemiluminescence method detection kit | |
CN111337682A (en) | Novel coronavirus IgM/IgG magnetic particle chemiluminescence immunoassay kit | |
JPH02503029A (en) | Use of polyoxyethylene ethers to improve the performance of immunoassays using peroxidase conjugates | |
CN101377504A (en) | Chemiluminescence immune analysis determination reagent kit for detecting Toxoplasma Gondi IgM antibody | |
Li et al. | Prevalence of serum antibodies to TORCH among women before pregnancy or in the early period of pregnancy in Beijing | |
CN108195815A (en) | Time-resolved fluoroimmunoassay chromatography detects test strips, kit and the method for AMH | |
AU621310B2 (en) | Immunometric assay kit and method applicable to whole cells | |
CN108519487A (en) | A kind of quantitative detecting method and detection kit of interleukin-6 | |
CN110346557A (en) | A kind of detection kit | |
CN109470862A (en) | Method of immunity, the system for identifying immunoassays and kit | |
CN108037283B (en) | A kind of antibody diluent and its preparation method and application for enzyme linked immunosorbent detection | |
NO149864B (en) | IMMUNOLOGICAL REAGENT. | |
CN206038685U (en) | Immunity side direction chromatography detecting system | |
CN109470856A (en) | Method of immunity, the system for identifying immunoassays and kit | |
CN108089005A (en) | A kind of magnetic particle alpha-fetoprotein chemiluminescence immune detection reagent kit and preparation method thereof | |
CN107490695B (en) | The board-like chemoluminescence method detection kit and preparation method of Carbohydrate Antigen 50 | |
CN104849455B (en) | A kind of based on Syphilis serum test protein chip preparation and application | |
CN109613261A (en) | The quickly kit of detection neutrophil gelatinase-associated lipocalin | |
CN106645715B (en) | It is a kind of for the protein-chip of 1 protein antibodies joint-detection of Epstein-Barr virus capsid antigen and nuclear antigen and its preparation and application | |
CN109212196A (en) | A kind of kit of quick detection free estriol | |
CN113588960A (en) | Immunochromatography detection test strip by ratio fluorescence method and detection method thereof | |
CN206573587U (en) | immune lateral chromatography detecting system | |
CN111089961A (en) | Toxoplasma IgM antibody detection kit and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |